AlfalfaGEDB | MS.gene062849

M. sativa cultivar XinJiangDaYe / MS.gene062849

KEGG: K00799
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF02798.23 PF13410.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062849.t1	RHN74659.1	96.8	219	7	0	1	219	1	219	1.20E-122	449.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062849.t1	Q03666	72.9	218	58	1	3	219	4	221	1.9e-94	346.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062849.t1	A0A396J8T0	96.8	219	7	0	1	219	1	219	8.3e-123	449.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049351	MS.gene062849	0.83777	4.27E-57	-1.69E-46
MS.gene049491	MS.gene062849	0.855427	6.35E-62	-1.69E-46
MS.gene049614	MS.gene062849	0.804702	1.90E-49	-1.69E-46
MS.gene049661	MS.gene062849	0.845072	5.10E-59	-1.69E-46
MS.gene049662	MS.gene062849	0.84227	2.87E-58	-1.69E-46
MS.gene049681	MS.gene062849	0.832222	1.07E-55	-1.69E-46
MS.gene049758	MS.gene062849	0.840576	8.00E-58	-1.69E-46
MS.gene050076	MS.gene062849	0.821979	3.03E-53	-1.69E-46
MS.gene050128	MS.gene062849	0.812251	4.66E-51	-1.69E-46
MS.gene050301	MS.gene062849	0.869843	2.28E-66	-1.69E-46
MS.gene050397	MS.gene062849	0.827261	1.73E-54	-1.69E-46
MS.gene050432	MS.gene062849	0.810196	1.30E-50	-1.69E-46
MS.gene050767	MS.gene062849	0.804011	2.65E-49	-1.69E-46
MS.gene050770	MS.gene062849	0.806844	6.75E-50	-1.69E-46
MS.gene050971	MS.gene062849	0.808668	2.77E-50	-1.69E-46
MS.gene05101	MS.gene062849	0.858086	1.05E-62	-1.69E-46
MS.gene05105	MS.gene062849	0.808751	2.65E-50	-1.69E-46
MS.gene051255	MS.gene062849	0.822073	2.88E-53	-1.69E-46
MS.gene051360	MS.gene062849	0.809869	1.53E-50	-1.69E-46
MS.gene051471	MS.gene062849	0.816563	5.18E-52	-1.69E-46
MS.gene052078	MS.gene062849	0.842203	2.98E-58	-1.69E-46
MS.gene052178	MS.gene062849	0.835657	1.48E-56	-1.69E-46
MS.gene052188	MS.gene062849	0.854948	8.76E-62	-1.69E-46
MS.gene052207	MS.gene062849	0.800511	1.39E-48	-1.69E-46
MS.gene052253	MS.gene062849	0.831578	1.54E-55	-1.69E-46
MS.gene052287	MS.gene062849	0.822367	2.46E-53	-1.69E-46
MS.gene052290	MS.gene062849	0.80679	6.93E-50	-1.69E-46
MS.gene052310	MS.gene062849	0.83653	8.87E-57	-1.69E-46
MS.gene052322	MS.gene062849	0.806353	8.57E-50	-1.69E-46
MS.gene052326	MS.gene062849	0.838435	2.88E-57	-1.69E-46
MS.gene052340	MS.gene062849	0.84371	1.19E-58	-1.69E-46
MS.gene052421	MS.gene062849	0.800136	1.66E-48	-1.69E-46
MS.gene052485	MS.gene062849	0.817711	2.86E-52	-1.69E-46
MS.gene05296	MS.gene062849	0.802209	6.25E-49	-1.69E-46
MS.gene053209	MS.gene062849	0.859209	4.84E-63	-1.69E-46
MS.gene053557	MS.gene062849	0.814387	1.58E-51	-1.69E-46
MS.gene053758	MS.gene062849	0.839715	1.34E-57	-1.69E-46
MS.gene053813	MS.gene062849	0.822669	2.09E-53	-1.69E-46
MS.gene053836	MS.gene062849	0.843502	1.35E-58	-1.69E-46
MS.gene054010	MS.gene062849	0.811908	5.53E-51	-1.69E-46
MS.gene054011	MS.gene062849	0.834597	2.73E-56	-1.69E-46
MS.gene054012	MS.gene062849	0.827325	1.67E-54	-1.69E-46
MS.gene054013	MS.gene062849	0.826255	3.00E-54	-1.69E-46
MS.gene054123	MS.gene062849	0.82696	2.04E-54	-1.69E-46
MS.gene054376	MS.gene062849	0.818378	2.02E-52	-1.69E-46
MS.gene055116	MS.gene062849	0.818297	2.11E-52	-1.69E-46
MS.gene055277	MS.gene062849	0.885564	7.29E-72	-1.69E-46
MS.gene055390	MS.gene062849	0.834116	3.61E-56	-1.69E-46
MS.gene055412	MS.gene062849	0.80932	2.01E-50	-1.69E-46
MS.gene055422	MS.gene062849	0.849187	3.80E-60	-1.69E-46
MS.gene055428	MS.gene062849	0.842514	2.47E-58	-1.69E-46
MS.gene055604	MS.gene062849	0.832543	8.90E-56	-1.69E-46
MS.gene055666	MS.gene062849	0.800816	1.21E-48	-1.69E-46
MS.gene056191	MS.gene062849	0.868332	7.06E-66	-1.69E-46
MS.gene056314	MS.gene062849	0.807966	3.90E-50	-1.69E-46
MS.gene056410	MS.gene062849	0.840295	9.48E-58	-1.69E-46
MS.gene056609	MS.gene062849	0.864076	1.58E-64	-1.69E-46
MS.gene056612	MS.gene062849	0.814705	1.34E-51	-1.69E-46
MS.gene056659	MS.gene062849	0.81959	1.07E-52	-1.69E-46
MS.gene056931	MS.gene062849	0.842792	2.08E-58	-1.69E-46
MS.gene056933	MS.gene062849	0.837852	4.07E-57	-1.69E-46
MS.gene056935	MS.gene062849	0.829336	5.45E-55	-1.69E-46
MS.gene05702	MS.gene062849	0.840961	6.34E-58	-1.69E-46
MS.gene057123	MS.gene062849	0.817926	2.56E-52	-1.69E-46
MS.gene057245	MS.gene062849	0.80232	5.93E-49	-1.69E-46
MS.gene05733	MS.gene062849	0.86442	1.23E-64	-1.69E-46
MS.gene057450	MS.gene062849	0.800534	1.38E-48	-1.69E-46
MS.gene057749	MS.gene062849	0.819133	1.36E-52	-1.69E-46
MS.gene057782	MS.gene062849	0.802919	4.46E-49	-1.69E-46
MS.gene057796	MS.gene062849	0.822113	2.82E-53	-1.69E-46
MS.gene057823	MS.gene062849	0.814685	1.36E-51	-1.69E-46
MS.gene058002	MS.gene062849	0.832393	9.70E-56	-1.69E-46
MS.gene058082	MS.gene062849	0.802491	5.47E-49	-1.69E-46
MS.gene05811	MS.gene062849	0.844706	6.40E-59	-1.69E-46
MS.gene058233	MS.gene062849	0.800739	1.25E-48	-1.69E-46
MS.gene05842	MS.gene062849	0.808453	3.07E-50	-1.69E-46
MS.gene058660	MS.gene062849	0.865467	5.78E-65	-1.69E-46
MS.gene05886	MS.gene062849	0.836253	1.04E-56	-1.69E-46
MS.gene058928	MS.gene062849	0.847401	1.18E-59	-1.69E-46
MS.gene05907	MS.gene062849	0.847795	9.23E-60	-1.69E-46
MS.gene059294	MS.gene062849	0.816045	6.77E-52	-1.69E-46
MS.gene059518	MS.gene062849	0.826673	2.38E-54	-1.69E-46
MS.gene059536	MS.gene062849	0.801829	7.48E-49	-1.69E-46
MS.gene059597	MS.gene062849	0.858699	6.89E-63	-1.69E-46
MS.gene059676	MS.gene062849	0.830449	2.92E-55	-1.69E-46
MS.gene059688	MS.gene062849	0.860474	2.01E-63	-1.69E-46
MS.gene059692	MS.gene062849	0.865122	7.42E-65	-1.69E-46
MS.gene059695	MS.gene062849	0.862717	4.16E-64	-1.69E-46
MS.gene059711	MS.gene062849	0.836837	7.40E-57	-1.69E-46
MS.gene05984	MS.gene062849	0.840361	9.11E-58	-1.69E-46
MS.gene059894	MS.gene062849	0.851933	6.42E-61	-1.69E-46
MS.gene059974	MS.gene062849	0.864077	1.58E-64	-1.69E-46
MS.gene060005	MS.gene062849	0.816731	4.75E-52	-1.69E-46
MS.gene06037	MS.gene062849	0.858096	1.04E-62	-1.69E-46
MS.gene060559	MS.gene062849	0.841375	4.94E-58	-1.69E-46
MS.gene061049	MS.gene062849	0.861459	1.01E-63	-1.69E-46
MS.gene061051	MS.gene062849	-0.818217	2.20E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062849	MS.gene062844	PPI
MS.gene063437	MS.gene062849	PPI
MS.gene065586	MS.gene062849	PPI
MS.gene062849	MS.gene039180	PPI
MS.gene062849	MS.gene068678	PPI
MS.gene062849	MS.gene039179	PPI
MS.gene062849	MS.gene20711	PPI
MS.gene068681	MS.gene062849	PPI
MS.gene062849	MS.gene005621	PPI
MS.gene062849	MS.gene062846	PPI
MS.gene062849	MS.gene068679	PPI
MS.gene012776	MS.gene062849	PPI
MS.gene062849	MS.gene012777	PPI
MS.gene062848	MS.gene062849	PPI
MS.gene062847	MS.gene062849	PPI
MS.gene062849	MS.gene43462	PPI
MS.gene065582	MS.gene062849	PPI
MS.gene062849	MS.gene95066	PPI
MS.gene062849	MS.gene43463	PPI
MS.gene062849	MS.gene063436	PPI
MS.gene062849	MS.gene065585	PPI
MS.gene062849	MS.gene062845	PPI
MS.gene062849	MS.gene039178	PPI
MS.gene062849	MS.gene063432	PPI
MS.gene062849	MS.gene039175	PPI
MS.gene042063	MS.gene062849	PPI
MS.gene062849	MS.gene03034	PPI
MS.gene062849	MS.gene039177	PPI
MS.gene062849	MS.gene95067	PPI
MS.gene062850	MS.gene062849	PPI
MS.gene062849	MS.gene063430	PPI
MS.gene062849	MS.gene063433	PPI
MS.gene062849	MS.gene065582	PPI
MS.gene062849	MS.gene063431	PPI
MS.gene062849	MS.gene039174	PPI
MS.gene065587	MS.gene062849	PPI
MS.gene062849	MS.gene039181	PPI
MS.gene062849	MS.gene30529	PPI
MS.gene062849	MS.gene042064	PPI
MS.gene062849	MS.gene065587	PPI
MS.gene062849	MS.gene07192	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062849.t1	MTR_2g070150	93.627	204	13	0	1	204	1	204	1.95e-145	404
MS.gene062849.t1	MTR_2g070140	94.581	203	11	0	17	219	1	203	1.24e-144	401
MS.gene062849.t1	MTR_2g070180	85.047	214	32	0	1	214	1	214	6.88e-141	392
MS.gene062849.t1	MTR_2g070130	77.169	219	50	0	1	219	1	219	1.94e-133	374
MS.gene062849.t1	MTR_2g070210	75.113	221	53	1	1	219	1	221	2.83e-127	358
MS.gene062849.t1	MTR_2g070200	70.136	221	64	2	1	219	1	221	2.96e-117	333
MS.gene062849.t1	MTR_2g070060	65.753	219	75	0	1	219	1	219	5.26e-111	317
MS.gene062849.t1	MTR_3g467420	63.721	215	78	0	5	219	10	224	1.42e-106	306
MS.gene062849.t1	MTR_2g070070	64.840	219	77	0	1	219	1	219	3.58e-106	305
MS.gene062849.t1	MTR_8g056940	64.840	219	77	0	1	219	1	219	2.21e-102	295
MS.gene062849.t1	MTR_3g467430	59.908	217	87	0	3	219	8	224	1.52e-100	291
MS.gene062849.t1	MTR_2g070110	62.100	219	82	1	1	219	1	218	1.09e-99	288
MS.gene062849.t1	MTR_2g070120	61.972	213	81	0	1	213	1	213	3.18e-95	277
MS.gene062849.t1	MTR_4g124130	57.727	220	92	1	1	219	1	220	5.75e-93	271
MS.gene062849.t1	MTR_5g076900	52.511	219	102	2	2	219	4	221	2.31e-81	242
MS.gene062849.t1	MTR_0186s0030	66.667	141	47	0	1	141	1	141	7.02e-64	195
MS.gene062849.t1	MTR_1g090060	44.762	210	111	3	4	209	3	211	4.35e-56	178
MS.gene062849.t1	MTR_8g087425	41.475	217	124	2	2	216	3	218	3.61e-52	168
MS.gene062849.t1	MTR_4g059730	42.056	214	117	4	1	209	1	212	1.14e-50	164
MS.gene062849.t1	MTR_7g065740	44.340	212	110	5	3	209	6	214	1.23e-50	164
MS.gene062849.t1	MTR_4g019790	43.062	209	114	4	4	209	7	213	1.24e-49	161
MS.gene062849.t1	MTR_7g065720	42.453	212	114	6	3	209	6	214	5.54e-49	160
MS.gene062849.t1	MTR_1g090100	44.712	208	110	3	5	208	3	209	5.55e-49	160
MS.gene062849.t1	MTR_7g065750	43.062	209	113	5	3	207	6	212	6.56e-49	159
MS.gene062849.t1	MTR_7g065660	40.476	210	120	4	3	209	6	213	4.83e-48	157
MS.gene062849.t1	MTR_3g099757	40.488	205	119	2	3	205	4	207	4.92e-48	157
MS.gene062849.t1	MTR_5g037380	40.455	220	122	5	2	212	3	222	7.69e-48	157
MS.gene062849.t1	MTR_4g019780	38.756	209	125	2	3	209	7	214	6.47e-47	154
MS.gene062849.t1	MTR_5g040430	36.493	211	127	2	2	205	5	215	6.63e-47	154
MS.gene062849.t1	MTR_8g087410	41.667	204	116	2	4	205	6	208	1.11e-46	154
MS.gene062849.t1	MTR_1g090070	39.810	211	121	4	4	209	9	218	1.77e-46	154
MS.gene062849.t1	MTR_7g065630	39.524	210	118	5	3	209	24	227	2.41e-45	151
MS.gene062849.t1	MTR_7g065270	40.191	209	120	5	3	208	6	212	2.55e-45	150
MS.gene062849.t1	MTR_1g090150	38.288	222	123	6	3	216	2	217	5.16e-45	150
MS.gene062849.t1	MTR_7g065710	40.476	210	120	5	1	209	1	206	1.95e-44	148
MS.gene062849.t1	MTR_1g090090	42.105	209	116	4	4	208	3	210	5.01e-44	147
MS.gene062849.t1	MTR_7g065590	37.143	210	127	4	3	209	6	213	1.13e-43	146
MS.gene062849.t1	MTR_8g061950	37.736	212	123	3	3	205	4	215	1.64e-43	146
MS.gene062849.t1	MTR_7g065600	38.249	217	126	5	3	216	6	217	7.24e-43	144
MS.gene062849.t1	MTR_6g080440	39.791	191	110	3	18	205	17	205	9.63e-43	144
MS.gene062849.t1	MTR_7g065290	39.713	209	121	5	3	208	6	212	9.80e-43	144
MS.gene062849.t1	MTR_7g065265	38.756	209	123	5	3	208	6	212	9.65e-42	141
MS.gene062849.t1	MTR_7g065700	41.429	210	118	5	1	209	4	209	1.11e-41	141
MS.gene062849.t1	MTR_1g115195	39.336	211	119	5	1	205	1	208	2.40e-41	140
MS.gene062849.t1	MTR_7g065680	40.000	210	117	5	3	209	6	209	5.44e-41	139
MS.gene062849.t1	MTR_7g065230	38.462	208	120	5	3	208	6	207	9.42e-41	139
MS.gene062849.t1	MTR_7g065640	37.500	208	111	4	3	209	6	195	2.16e-40	137
MS.gene062849.t1	MTR_7g065260	40.957	188	106	5	3	187	13	198	1.11e-34	123
MS.gene062849.t1	MTR_2g072120	67.123	73	23	1	47	118	1	73	5.04e-31	109
MS.gene062849.t1	MTR_4g134370	29.545	176	97	5	3	159	14	181	3.29e-14	69.3
MS.gene062849.t1	MTR_4g134380	30.409	171	100	5	4	159	15	181	8.64e-14	68.2
MS.gene062849.t1	MTR_4g134380	30.303	165	96	5	4	153	15	175	1.37e-12	63.9
MS.gene062849.t1	MTR_4g043640	41.176	68	33	1	129	189	18	85	3.24e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062849.t1	AT1G78380	67.130	216	71	0	1	216	1	216	1.99e-110	316
MS.gene062849.t1	AT1G17180	63.927	219	79	0	1	219	1	219	4.29e-110	315
MS.gene062849.t1	AT1G78370	64.953	214	75	0	1	214	1	214	7.29e-106	304
MS.gene062849.t1	AT1G78340	63.551	214	78	0	1	214	1	214	9.70e-106	304
MS.gene062849.t1	AT1G17170	62.557	219	81	1	1	219	1	218	3.73e-105	302
MS.gene062849.t1	AT1G78360	62.273	220	82	1	1	219	1	220	1.19e-103	298
MS.gene062849.t1	AT1G78320	63.551	214	78	0	1	214	1	214	6.07e-102	294
MS.gene062849.t1	AT1G17190	57.209	215	92	0	3	217	4	218	2.09e-94	275
MS.gene062849.t1	AT1G53680	55.708	219	95	2	3	219	6	224	1.39e-92	271
MS.gene062849.t1	AT3G43800	53.704	216	96	3	3	214	4	219	1.38e-80	240
MS.gene062849.t1	AT1G78320	54.673	214	70	1	1	214	1	187	4.49e-80	238
MS.gene062849.t1	AT2G29420	41.463	205	117	2	2	204	7	210	9.28e-55	174
MS.gene062849.t1	AT2G29450	46.377	207	104	5	3	204	5	209	4.50e-52	167
MS.gene062849.t1	AT3G09270	40.758	211	120	4	3	209	5	214	3.17e-51	166
MS.gene062849.t1	AT2G29440	44.175	206	109	4	3	204	5	208	1.36e-50	164
MS.gene062849.t1	AT2G29490	40.654	214	121	4	3	212	6	217	3.67e-50	163
MS.gene062849.t1	AT1G10360	41.860	215	117	4	2	208	3	217	1.94e-48	159
MS.gene062849.t1	AT2G29480	39.631	217	125	4	3	215	6	220	2.98e-48	158
MS.gene062849.t1	AT1G59700	41.441	222	121	5	3	215	5	226	2.08e-47	156
MS.gene062849.t1	AT2G29460	37.788	217	129	4	3	215	6	220	6.90e-47	154
MS.gene062849.t1	AT2G29470	39.171	217	125	5	5	216	8	222	1.15e-46	154
MS.gene062849.t1	AT1G59670	43.049	223	116	5	3	215	5	226	2.23e-46	153
MS.gene062849.t1	AT1G10370	39.269	219	125	4	2	212	3	221	1.22e-45	151
MS.gene062849.t1	AT1G74590	34.081	223	133	3	3	215	6	224	1.25e-44	149
MS.gene062849.t1	AT1G27130	36.744	215	127	4	3	208	5	219	9.44e-42	141
MS.gene062849.t1	AT5G62480	34.978	223	133	4	3	214	7	228	1.58e-40	139
MS.gene062849.t1	AT1G69930	37.441	211	125	3	5	208	13	223	1.76e-40	138
MS.gene062849.t1	AT1G27140	39.906	213	118	4	3	205	5	217	2.14e-40	138
MS.gene062849.t1	AT1G69920	36.283	226	126	6	5	218	35	254	5.42e-38	132
MS.gene062849.t1	AT5G62480	32.571	175	82	3	3	168	7	154	6.17e-24	95.1
MS.gene062849.t1	AT5G62480	32.571	175	81	3	3	168	7	153	6.30e-24	95.1

Find 56 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATTCTACACTTGGTTTAA+AGG	0.105597	2.4:+25186743	MS.gene062849:CDS
AGAGATCACAAGCTAGATTT+TGG	0.158738	2.4:+25185761	MS.gene062849:CDS
GTGAAATAGATCTATGAAAT+TGG	0.256148	2.4:+25186575	MS.gene062849:intron
TTCAGTATATTGATGAAGTT+TGG	0.281776	2.4:+25185701	MS.gene062849:CDS
TACTTTGGAGGAGACAAAAT+TGG	0.303292	2.4:+25186698	MS.gene062849:CDS
AAGTGATTCTGCTAGATTAC+TGG	0.343179	2.4:+25185500	MS.gene062849:CDS
ATGAAATTGGAAAGAACCTT+TGG	0.349218	2.4:+25186588	MS.gene062849:CDS
CTCAACCTGCATGCATCTCT+TGG	0.377767	2.4:-25186825	None:intergenic
TTTCCATTATGAATGAGAAC+AGG	0.382184	2.4:-25185652	None:intergenic
ATCCCTGTTCTCATTCATAA+TGG	0.385871	2.4:+25185649	MS.gene062849:CDS
CAGAATTGCCCTAGCTGAAA+AGG	0.386828	2.4:+25185546	MS.gene062849:CDS
GCAATTAAAGATTCACAGAT+TGG	0.390407	2.4:-25185676	None:intergenic
CACTTATTCCATTCTACACT+TGG	0.395366	2.4:+25186735	MS.gene062849:CDS
GATTACTGGGCAAGTCCATT+TGG	0.423782	2.4:+25185514	MS.gene062849:CDS
CCATACACCTTATCTTGATC+AGG	0.427123	2.4:-25186866	None:intergenic
GGCTGATTATGTTGATAAGA+AGG	0.427339	2.4:+25185783	MS.gene062849:CDS
TTTAAAGGCTATGAGACCTT+TGG	0.432290	2.4:+25186758	MS.gene062849:CDS
AGATAAGGTGTATGGCTTCA+TGG	0.436683	2.4:+25186874	MS.gene062849:CDS
TTGGCCCAAGCAATGAACTT+AGG	0.437475	2.4:-25186806	None:intergenic
CTGGGAGACAAGACTTACTT+TGG	0.447631	2.4:+25186683	MS.gene062849:CDS
CGTAGAAGCTCATAGATTGT+TGG	0.452212	2.4:+25186652	MS.gene062849:CDS
TAGATTGTTGGAGCAAGAGC+TGG	0.455234	2.4:+25186664	MS.gene062849:CDS
TGGCCCAAGCAATGAACTTA+GGG	0.464217	2.4:-25186805	None:intergenic
ATGCCCTAAGTTCATTGCTT+GGG	0.465024	2.4:+25186802	MS.gene062849:CDS
ATGGCTTCATGGTGGAGATC+AGG	0.466419	2.4:+25186885	MS.gene062849:CDS
CTAGCTTGTGATCTCTGATA+AGG	0.467136	2.4:-25185754	None:intergenic
AATGCCCTAAGTTCATTGCT+TGG	0.469149	2.4:+25186801	MS.gene062849:CDS
AGAACAAGAAGCTGCGAAGA+AGG	0.470283	2.4:+25186625	MS.gene062849:CDS
GTGGAGATCAGGAAGAGGTT+TGG	0.487397	2.4:+25186896	MS.gene062849:CDS
AAGAACCTTTGGACCAGAAA+AGG	0.491905	2.4:+25186599	MS.gene062849:CDS
CCTTTAAACCAAGTGTAGAA+TGG	0.500913	2.4:-25186743	None:intergenic
ATTCTGACCCTCATGCCAAA+TGG	0.501493	2.4:-25185529	None:intergenic
CCTGATCAAGATAAGGTGTA+TGG	0.506244	2.4:+25186866	MS.gene062849:CDS
GGAGACAAGACTTACTTTGG+AGG	0.510834	2.4:+25186686	MS.gene062849:CDS
GGTTCATTTGAAGTAACAAA+GGG	0.524302	2.4:-25185612	None:intergenic
GGCAAGTCCATTTGGCATGA+GGG	0.542919	2.4:+25185522	MS.gene062849:CDS
CTCTACATTGATGTTGCCAA+AGG	0.546992	2.4:-25186774	None:intergenic
AGAATTGCCCTAGCTGAAAA+GGG	0.551173	2.4:+25185547	MS.gene062849:CDS
AGTGATTCTGCTAGATTACT+GGG	0.559493	2.4:+25185501	MS.gene062849:CDS
GAAGAGGTTTGGCCTTGAGT+AGG	0.568829	2.4:+25186907	MS.gene062849:CDS
GTCGGTCCCTGATCAAGATA+AGG	0.571607	2.4:+25186859	MS.gene062849:CDS
TGGCAACATCAATGTAGAGA+AGG	0.574601	2.4:+25186778	MS.gene062849:CDS
GGGTTCATTTGAAGTAACAA+AGG	0.580169	2.4:-25185613	None:intergenic
CAGAATCACTTCATCAGCCA+TGG	0.584359	2.4:-25185488	None:intergenic
GGTTGAGAGTGTTTCCAAGT+CGG	0.584489	2.4:+25186841	MS.gene062849:CDS
GGGCAAGTCCATTTGGCATG+AGG	0.587485	2.4:+25185521	MS.gene062849:CDS
TTGGGCCAAGAGATGCATGC+AGG	0.591022	2.4:+25186820	MS.gene062849:CDS
TCATGGTGGAGATCAGGAAG+AGG	0.595602	2.4:+25186891	MS.gene062849:CDS
GATCTCTGATAAGGATCAGA+AGG	0.596783	2.4:-25185745	None:intergenic
TTCCATTATGAATGAGAACA+GGG	0.600232	2.4:-25185651	None:intergenic
AGATTGTTGGAGCAAGAGCT+GGG	0.600824	2.4:+25186665	MS.gene062849:CDS
TAAGGATCAGAAGGCAACAA+AGG	0.613337	2.4:-25185736	None:intergenic
ATCTTGATCAGGGACCGACT+TGG	0.617812	2.4:-25186855	None:intergenic
TAACATCTCCAACCTACTCA+AGG	0.623527	2.4:-25186919	None:intergenic
CATACACCTTATCTTGATCA+GGG	0.625348	2.4:-25186865	None:intergenic
TAAGGTGTATGGCTTCATGG+TGG	0.710103	2.4:+25186877	MS.gene062849:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTTATATAAACTATAAG+TGG	-	chr2.4:25186260-25186279	None:intergenic	15.0%
!!	AGAATAAAATTTCATCAAAA+AGG	-	chr2.4:25185898-25185917	None:intergenic	15.0%
!!!	CATATAATTGATTCATATTA+AGG	-	chr2.4:25186401-25186420	None:intergenic	15.0%
!!!	TATTTTGAACAATTTATTAG+AGG	+	chr2.4:25186099-25186118	MS.gene062849:intron	15.0%
!!	AATAACTTAATAGCTTAGTT+AGG	-	chr2.4:25186364-25186383	None:intergenic	20.0%
!!	TAAGTTATTTATCCAAACAA+GGG	+	chr2.4:25186375-25186394	MS.gene062849:intron	20.0%
!!	TTAAGTTATTTATCCAAACA+AGG	+	chr2.4:25186374-25186393	MS.gene062849:intron	20.0%
!!!	GTTTTTTCATGAGAAAATAT+AGG	+	chr2.4:25186021-25186040	MS.gene062849:intron	20.0%
!	ACATGTAATGAATCATACAA+AGG	-	chr2.4:25185826-25185845	None:intergenic	25.0%
!	AGCAAAAAGAAGAAGATTTA+AGG	+	chr2.4:25185581-25185600	MS.gene062849:CDS	25.0%
!	ATTTCATCAAAAAGGACAAA+AGG	-	chr2.4:25185890-25185909	None:intergenic	25.0%
!	ATTTGAAGATAGTTGAGATT+TGG	+	chr2.4:25186430-25186449	MS.gene062849:intron	25.0%
!	CATGTAATGAATCATACAAA+GGG	-	chr2.4:25185825-25185844	None:intergenic	25.0%
!	GTGAAATAGATCTATGAAAT+TGG	+	chr2.4:25186575-25186594	MS.gene062849:intron	25.0%
!	TTCAGTATATTGATGAAGTT+TGG	+	chr2.4:25185701-25185720	MS.gene062849:CDS	25.0%
!	TTTCATCAAAAAGGACAAAA+GGG	-	chr2.4:25185889-25185908	None:intergenic	25.0%
!	TTTCTCATGAAAAAACTTAC+AGG	-	chr2.4:25186017-25186036	None:intergenic	25.0%
!!	GCTTAGAATTTGTTTATCAT+TGG	+	chr2.4:25185854-25185873	MS.gene062849:intron	25.0%
!!!	CTAATTGAAATGTGCTTTTT+CGG	+	chr2.4:25186197-25186216	MS.gene062849:intron	25.0%
	AACTTAATAGCTTAGTTAGG+TGG	-	chr2.4:25186361-25186380	None:intergenic	30.0%
	ATGAAATTGGAAAGAACCTT+TGG	+	chr2.4:25186588-25186607	MS.gene062849:CDS	30.0%
	GCAATTAAAGATTCACAGAT+TGG	-	chr2.4:25185679-25185698	None:intergenic	30.0%
	TATTAGCAAGTATTAGTCCT+TGG	+	chr2.4:25186496-25186515	MS.gene062849:intron	30.0%
	TTCCATTATGAATGAGAACA+GGG	-	chr2.4:25185654-25185673	None:intergenic	30.0%
	TTTCCATTATGAATGAGAAC+AGG	-	chr2.4:25185655-25185674	None:intergenic	30.0%
!	GGTTCATTTGAAGTAACAAA+GGG	-	chr2.4:25185615-25185634	None:intergenic	30.0%
	AAGAAAAACAACAGTCACCA+AGG	-	chr2.4:25186516-25186535	None:intergenic	35.0%
	AAGTGATTCTGCTAGATTAC+TGG	+	chr2.4:25185500-25185519	MS.gene062849:CDS	35.0%
	AGTGATTCTGCTAGATTACT+GGG	+	chr2.4:25185501-25185520	MS.gene062849:CDS	35.0%
	ATAGGACTGTGCATTTAACT+TGG	+	chr2.4:25186039-25186058	MS.gene062849:intron	35.0%
	ATCCCTGTTCTCATTCATAA+TGG	+	chr2.4:25185649-25185668	MS.gene062849:CDS	35.0%
	CACTTATTCCATTCTACACT+TGG	+	chr2.4:25186735-25186754	MS.gene062849:CDS	35.0%
	CATACACCTTATCTTGATCA+GGG	-	chr2.4:25186868-25186887	None:intergenic	35.0%
	CCATTCTACACTTGGTTTAA+AGG	+	chr2.4:25186743-25186762	MS.gene062849:CDS	35.0%
	CCTTTAAACCAAGTGTAGAA+TGG	-	chr2.4:25186746-25186765	None:intergenic	35.0%
	TATGCTCTCAAAGATAGCTT+AGG	+	chr2.4:25186230-25186249	MS.gene062849:intron	35.0%
	TTCATATTAAGGCCCTTGTT+TGG	-	chr2.4:25186390-25186409	None:intergenic	35.0%
	TTTAAAGGCTATGAGACCTT+TGG	+	chr2.4:25186758-25186777	MS.gene062849:CDS	35.0%
!	ACAGGGATTTTCTTGTGAAT+AGG	-	chr2.4:25185637-25185656	None:intergenic	35.0%
!	AGAGATCACAAGCTAGATTT+TGG	+	chr2.4:25185761-25185780	MS.gene062849:CDS	35.0%
!	CAGGGATTTTCTTGTGAATA+GGG	-	chr2.4:25185636-25185655	None:intergenic	35.0%
!	GAGATCACAAGCTAGATTTT+GGG	+	chr2.4:25185762-25185781	MS.gene062849:CDS	35.0%
!	GGCTGATTATGTTGATAAGA+AGG	+	chr2.4:25185783-25185802	MS.gene062849:CDS	35.0%
!	GGGTTCATTTGAAGTAACAA+AGG	-	chr2.4:25185616-25185635	None:intergenic	35.0%
!	TACTTTGGAGGAGACAAAAT+TGG	+	chr2.4:25186698-25186717	MS.gene062849:CDS	35.0%
!	TTCTTGTTCTTCTCCTTTTC+TGG	-	chr2.4:25186615-25186634	None:intergenic	35.0%
!!	TGGATATTTTTCAGCTTGCA+TGG	+	chr2.4:25186469-25186488	MS.gene062849:intron	35.0%
!!!	AGGAGACAAAATTGGTTTTG+TGG	+	chr2.4:25186706-25186725	MS.gene062849:CDS	35.0%
	AAGAACCTTTGGACCAGAAA+AGG	+	chr2.4:25186599-25186618	MS.gene062849:CDS	40.0%
	AATGCCCTAAGTTCATTGCT+TGG	+	chr2.4:25186801-25186820	MS.gene062849:CDS	40.0%
	AGAATTGCCCTAGCTGAAAA+GGG	+	chr2.4:25185547-25185566	MS.gene062849:CDS	40.0%
	AGATAAGGTGTATGGCTTCA+TGG	+	chr2.4:25186874-25186893	MS.gene062849:CDS	40.0%
	ATGCCCTAAGTTCATTGCTT+GGG	+	chr2.4:25186802-25186821	MS.gene062849:CDS	40.0%
	CCATACACCTTATCTTGATC+AGG	-	chr2.4:25186869-25186888	None:intergenic	40.0%
	CCTGATCAAGATAAGGTGTA+TGG	+	chr2.4:25186866-25186885	MS.gene062849:CDS	40.0%
	CGTAGAAGCTCATAGATTGT+TGG	+	chr2.4:25186652-25186671	MS.gene062849:CDS	40.0%
	CTCTACATTGATGTTGCCAA+AGG	-	chr2.4:25186777-25186796	None:intergenic	40.0%
	GATCTCTGATAAGGATCAGA+AGG	-	chr2.4:25185748-25185767	None:intergenic	40.0%
	TAAGGATCAGAAGGCAACAA+AGG	-	chr2.4:25185739-25185758	None:intergenic	40.0%
	TGGCAACATCAATGTAGAGA+AGG	+	chr2.4:25186778-25186797	MS.gene062849:CDS	40.0%
	TTGGAACACTTACGAGCTAT+TGG	+	chr2.4:25186449-25186468	MS.gene062849:intron	40.0%
!	CTAGCTTGTGATCTCTGATA+AGG	-	chr2.4:25185757-25185776	None:intergenic	40.0%
!	CTTGATACCCTTTTCAGCTA+GGG	-	chr2.4:25185557-25185576	None:intergenic	40.0%
	AGAACAAGAAGCTGCGAAGA+AGG	+	chr2.4:25186625-25186644	MS.gene062849:CDS	45.0%
	AGATTGTTGGAGCAAGAGCT+GGG	+	chr2.4:25186665-25186684	MS.gene062849:CDS	45.0%
	ATTCTGACCCTCATGCCAAA+TGG	-	chr2.4:25185532-25185551	None:intergenic	45.0%
	CAGAATTGCCCTAGCTGAAA+AGG	+	chr2.4:25185546-25185565	MS.gene062849:CDS	45.0%
	CTGGGAGACAAGACTTACTT+TGG	+	chr2.4:25186683-25186702	MS.gene062849:CDS	45.0%
	GATTACTGGGCAAGTCCATT+TGG	+	chr2.4:25185514-25185533	MS.gene062849:CDS	45.0%
	GGAGACAAGACTTACTTTGG+AGG	+	chr2.4:25186686-25186705	MS.gene062849:CDS	45.0%
	TAAGGTGTATGGCTTCATGG+TGG	+	chr2.4:25186877-25186896	MS.gene062849:CDS	45.0%
	TAGATTGTTGGAGCAAGAGC+TGG	+	chr2.4:25186664-25186683	MS.gene062849:CDS	45.0%
	TGGCCCAAGCAATGAACTTA+GGG	-	chr2.4:25186808-25186827	None:intergenic	45.0%
	TTGGCCCAAGCAATGAACTT+AGG	-	chr2.4:25186809-25186828	None:intergenic	45.0%
!	GCTTGATACCCTTTTCAGCT+AGG	-	chr2.4:25185558-25185577	None:intergenic	45.0%
!!	CTTCTCCTTTTCTGGTCCAA+AGG	-	chr2.4:25186607-25186626	None:intergenic	45.0%
!!	GGTTGAGAGTGTTTCCAAGT+CGG	+	chr2.4:25186841-25186860	MS.gene062849:CDS	45.0%
	ATCTTGATCAGGGACCGACT+TGG	-	chr2.4:25186858-25186877	None:intergenic	50.0%
	CTCAACCTGCATGCATCTCT+TGG	-	chr2.4:25186828-25186847	None:intergenic	50.0%
	GGCAAGTCCATTTGGCATGA+GGG	+	chr2.4:25185522-25185541	MS.gene062849:CDS	50.0%
	GTCGGTCCCTGATCAAGATA+AGG	+	chr2.4:25186859-25186878	MS.gene062849:CDS	50.0%
	GTGGAGATCAGGAAGAGGTT+TGG	+	chr2.4:25186896-25186915	MS.gene062849:CDS	50.0%
!	ATGGCTTCATGGTGGAGATC+AGG	+	chr2.4:25186885-25186904	MS.gene062849:CDS	50.0%
!	TCATGGTGGAGATCAGGAAG+AGG	+	chr2.4:25186891-25186910	MS.gene062849:CDS	50.0%
	GGGCAAGTCCATTTGGCATG+AGG	+	chr2.4:25185521-25185540	MS.gene062849:CDS	55.0%
	TTGGGCCAAGAGATGCATGC+AGG	+	chr2.4:25186820-25186839	MS.gene062849:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	25185490	25186928	25185490	ID=MS.gene062849
chr2.4	mRNA	25185490	25186928	25185490	ID=MS.gene062849.t1;Parent=MS.gene062849
chr2.4	exon	25185490	25185804	25185490	ID=MS.gene062849.t1.exon1;Parent=MS.gene062849.t1
chr2.4	CDS	25185490	25185804	25185490	ID=cds.MS.gene062849.t1;Parent=MS.gene062849.t1
chr2.4	exon	25186584	25186928	25186584	ID=MS.gene062849.t1.exon2;Parent=MS.gene062849.t1
chr2.4	CDS	25186584	25186928	25186584	ID=cds.MS.gene062849.t1;Parent=MS.gene062849.t1

Gene Sequence

>MS.gene062849

ATGGCTGATGAAGTGATTCTGCTAGATTACTGGGCAAGTCCATTTGGCATGAGGGTCAGAATTGCCCTAGCTGAAAAGGGTATCAAGCATGAGCAAAAAGAAGAAGATTTAAGGAACAAGAGCCCTTTGTTACTTCAAATGAACCCTATTCACAAGAAAATCCCTGTTCTCATTCATAATGGAAAACCAATCTGTGAATCTTTAATTGCTGTTCAGTATATTGATGAAGTTTGGAATGAGAAATCTCCTTTGTTGCCTTCTGATCCTTATCAGAGATCACAAGCTAGATTTTGGGCTGATTATGTTGATAAGAAGGTATATAAATTTACATACCCTTTGTATGATTCATTACATGTTGTTTGTTGCTTAGAATTTGTTTATCATTGGTTTTGTTGACCCTTTTGTCCTTTTTGATGAAATTTTATTCTTTAATTAATTCTTTTGCATGTGATTGTTTGTGCTTCTTTTACATTTCTGAAATTTAAGTTTTATTTATTTAGTTTACATTTTTTTTTAAATTATTACCTGTAAGTTTTTTCATGAGAAAATATAGGACTGTGCATTTAACTTGGAGCTTATTTTTCTCTCAAAAAAAAAAAAACTTAAGCTTATTTTGAACAATTTATTAGAGGTTTGTAGCATTTGTGAGAATGTTTTCTGAGAGTTAAGCAAAACAGCTTATTCATATGTCTATAATCTGCTTTTAGCTAATTGAAATGTGCTTTTTCGGCTTAATTTCATATGCTCTCAAAGATAGCTTAGGAAAACCACTTATAGTTTATATAAACTTATAGATAAGTTAATCTATGACAAATAACGTTTGTTATAAAAATCATTTATATTTGAGCACTTCTATGATAACAAGTAGCCACCTAACTAAGCTATTAAGTTATTTATCCAAACAAGGGCCTTAATATGAATCAATTATATGTTTCTAAGTATTTGAAGATAGTTGAGATTTGGAACACTTACGAGCTATTGGATATTTTTCAGCTTGCATGGTCACTATTAGCAAGTATTAGTCCTTGGTGACTGTTGTTTTTCTTGTTATTACATTGATGATCTAATTGTGTGTGATTCTCATTGTGAAATAGATCTATGAAATTGGAAAGAACCTTTGGACCAGAAAAGGAGAAGAACAAGAAGCTGCGAAGAAGGAATTCGTAGAAGCTCATAGATTGTTGGAGCAAGAGCTGGGAGACAAGACTTACTTTGGAGGAGACAAAATTGGTTTTGTGGATGTAGCACTTATTCCATTCTACACTTGGTTTAAAGGCTATGAGACCTTTGGCAACATCAATGTAGAGAAGGAATGCCCTAAGTTCATTGCTTGGGCCAAGAGATGCATGCAGGTTGAGAGTGTTTCCAAGTCGGTCCCTGATCAAGATAAGGTGTATGGCTTCATGGTGGAGATCAGGAAGAGGTTTGGCCTTGAGTAG

Protein sequence

>MS.gene062849.t1

MADEVILLDYWASPFGMRVRIALAEKGIKHEQKEEDLRNKSPLLLQMNPIHKKIPVLIHNGKPICESLIAVQYIDEVWNEKSPLLPSDPYQRSQARFWADYVDKKIYEIGKNLWTRKGEEQEAAKKEFVEAHRLLEQELGDKTYFGGDKIGFVDVALIPFYTWFKGYETFGNINVEKECPKFIAWAKRCMQVESVSKSVPDQDKVYGFMVEIRKRFGLE