AlfalfaGEDB | MS.gene065582

M. sativa cultivar XinJiangDaYe / MS.gene065582

KEGG: K00799
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF02798.23 PF13410.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065582.t1	XP_013464343.1	96.3	219	8	0	1	219	1	219	3.70E-121	444.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065582.t1	P46417	82.4	216	38	0	1	216	1	216	1.4e-105	383.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065582.t1	B7FGX5	96.3	219	8	0	1	219	1	219	2.7e-121	444.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene012776	MS.gene065582	PPI
MS.gene042063	MS.gene065582	PPI
MS.gene062847	MS.gene065582	PPI
MS.gene062848	MS.gene065582	PPI
MS.gene062849	MS.gene065582	PPI
MS.gene062850	MS.gene065582	PPI
MS.gene063437	MS.gene065582	PPI
MS.gene065582	MS.gene012777	PPI
MS.gene065582	MS.gene43462	PPI
MS.gene065582	MS.gene95066	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065582.t1	MTR_2g070070	96.347	219	8	0	1	219	1	219	1.84e-160	442
MS.gene065582.t1	MTR_2g070060	87.215	219	28	0	1	219	1	219	2.11e-144	401
MS.gene065582.t1	MTR_2g070110	87.215	219	27	1	1	219	1	218	9.30e-134	375
MS.gene065582.t1	MTR_2g070180	66.355	214	72	0	1	214	1	214	7.80e-112	319
MS.gene065582.t1	MTR_2g070150	69.118	204	63	0	1	204	1	204	1.08e-110	316
MS.gene065582.t1	MTR_2g070140	66.502	203	68	0	17	219	1	203	1.33e-107	308
MS.gene065582.t1	MTR_2g070130	62.557	219	82	0	1	219	1	219	2.61e-107	308
MS.gene065582.t1	MTR_8g056940	65.753	219	75	0	1	219	1	219	6.34e-107	306
MS.gene065582.t1	MTR_2g070210	62.443	221	81	1	1	219	1	221	3.73e-104	300
MS.gene065582.t1	MTR_2g070200	61.818	220	82	2	1	218	1	220	3.50e-102	295

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065582.t1	AT1G78380	68.224	214	68	0	1	214	1	214	2.37e-110	315
MS.gene065582.t1	AT1G17180	64.840	219	77	0	1	219	1	219	9.54e-110	314
MS.gene065582.t1	AT1G17170	63.470	219	79	1	1	219	1	218	5.09e-103	297
MS.gene065582.t1	AT1G78340	63.551	214	78	0	1	214	1	214	1.17e-102	296
MS.gene065582.t1	AT1G78370	62.791	215	80	0	1	215	1	215	1.70e-102	295
MS.gene065582.t1	AT1G78360	62.727	220	81	1	1	219	1	220	4.73e-100	289
MS.gene065582.t1	AT1G78320	62.273	220	82	1	1	219	1	220	1.28e-98	286
MS.gene065582.t1	AT1G17190	60.748	214	84	0	4	217	5	218	3.62e-96	280
MS.gene065582.t1	AT1G53680	55.708	219	95	2	3	219	6	224	4.88e-90	264
MS.gene065582.t1	AT3G43800	53.241	216	97	3	4	215	5	220	1.89e-80	240

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Find 42 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTGAAGAGCATGAGGA+AGG	0.578570	2.2:-21900564	MS.gene065582:CDS
AAAGTGGTACCACCGTAAAA+AGG	0.389095	2.2:+21900486	None:intergenic
AACTCTTCCTGATCAAATGA+AGG	0.495473	2.2:-21900322	MS.gene065582:CDS
AAGACACTAGAGGAAACTTT+AGG	0.363941	2.2:-21900516	MS.gene065582:CDS
AAGGTGAAGAGCATGAGGAA+GGG	0.585110	2.2:-21900563	MS.gene065582:CDS
AATGTCAAGAAACCCAAAAG+TGG	0.577947	2.2:+21900470	None:intergenic
ACTGGAATCTTCTTATGAAT+TGG	0.280289	2.2:+21901369	None:intergenic
AGAGAGCTCAAGCTAGATTT+TGG	0.158333	2.2:-21901242	MS.gene065582:CDS
AGGAAAATGTGGCTTTCAAA+AGG	0.453535	2.2:-21900582	MS.gene065582:CDS
AGGATTGCATTAGCTGAAAA+GGG	0.508267	2.2:-21901456	MS.gene065582:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCACACAAATTACCAAA+AGG	+	chr2.2:21900732-21900751	None:intergenic	30.0%
ACTGGAATCTTCTTATGAAT+TGG	+	chr2.2:21900422-21900441	None:intergenic	30.0%
ATCAAAACCCACCAAAATTA+AGG	+	chr2.2:21900630-21900649	None:intergenic	30.0%
ATTCCAGTTCTTATCCATAA+TGG	-	chr2.2:21900434-21900453	MS.gene065582:CDS	30.0%
CATAAAACCAGCTGTAAAAA+GGG	+	chr2.2:21901352-21901371	None:intergenic	30.0%
TATTGTGCAGTATATAGATG+AGG	-	chr2.2:21900481-21900500	MS.gene065582:CDS	30.0%
TTTCCATTATGGATAAGAAC+TGG	+	chr2.2:21900440-21900459	None:intergenic	30.0%
AACTCTTCCTGATCAAATGA+AGG	-	chr2.2:21901466-21901485	MS.gene065582:CDS	35.0%
AAGACACTAGAGGAAACTTT+AGG	-	chr2.2:21901272-21901291	MS.gene065582:CDS	35.0%
AATGTCAAGAAACCCAAAAG+TGG	+	chr2.2:21901321-21901340	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	21900275	21901535	21900275	ID=MS.gene065582
chr2.2	mRNA	21900275	21901535	21900275	ID=MS.gene065582.t1;Parent=MS.gene065582
chr2.2	exon	21901221	21901535	21901221	ID=MS.gene065582.t1.exon1;Parent=MS.gene065582.t1
chr2.2	CDS	21901221	21901535	21901221	ID=cds.MS.gene065582.t1;Parent=MS.gene065582.t1
chr2.2	exon	21900275	21900619	21900275	ID=MS.gene065582.t1.exon2;Parent=MS.gene065582.t1
chr2.2	CDS	21900275	21900619	21900275	ID=cds.MS.gene065582.t1;Parent=MS.gene065582.t1

Gene Sequence

>MS.gene065582

ATGGCAACAGAGGTTGTTTTGTTGGATACATGGGTTAGCATGTTTGGTATGAGGGTTAGGATTGCATTAGCTGAAAAGGGAGTTAAATATGAGTACAAAGAAGAAAATCTTAGAAATAAGAGTCCTTTACTTCTTCAGATGAATCCAATTCATAAGAAGATTCCAGTTCTTATCCATAATGGAAAACCTATTTGTGAATCTGCTATTATTGTGCAGTATATAGATGAGGTTTGGAATGATAAAGCTCCAATCATGCCTTCTGATCCTTATGAGAGAGCTCAAGCTAGATTTTGGGTTGATTATATTGATAAAAAGGTAACTTACAATTGATTTGTTTTTCTTATATTTTCATCCTTAATTTTGGTGGGTTTTGATCAAAATTTTGAGATTCATGTTCTATTTTGTATATGTTAATTCTTCATGATTAACATTGGTAATTTTTGTGCTAGATTTACCTTTTGGTAATTTGTGTGCTTTTAAATCTCCTAGTACTTGAATCTTATTAAAAATCAAATTATTTGAAAGTACGAATGTAAATTATATAATTCAATTAGTACTTGAATCTTATTAAAAATCAAATTATTTGAAAGTACGAATGTAAATATAAATACTTGCGACACTTTCTTAGACAACTTATTAAAATAATTTATGACATGTTTATATGTTGTTTACGTCTTATTTTTATAAGACGGAAAAACTTTAAATCCTCTCATAACGGATGACTACCAAAGCTCCAAATATAACAATCTATATTCATAACTTCACAACTTTTTTTATCATTTTTTGTAGAAATAACAATCATGTACATAAGTGTTTATGCTATAAGCTTTTAATTAAATTGTTTATCCACATAATCATTCTTTGATCATATATATATAAAAAAAAAATTAAAAATCATTACTGATTTTGAATGCAGGTGTATGATACTTGGAGGAAAATGTGGCTTTCAAAAGGTGAAGAGCATGAGGAAGGGAAGAAAGAATTGATCTCTATTTTTAAGACACTAGAGGAAACTTTAGGTGACAAGCCTTTTTACGGTGGTACCACTTTTGGGTTTCTTGACATTGGTCTAATCCCTTTTTACAGCTGGTTTTATGCTTATGAGACATATGGTAACTTTAAAATGGAAGTGGAGTGTCCTAAACTCATGGCATGGGCCAAGAGATGCTTGGAGAAAGAGAGTGTTTCAAAAACTCTTCCTGATCAAATGAAGGTGTATGATTTTGTTGTGAGCATGAAGAAAACACTTGGTTTGGATTAG

Protein sequence

>MS.gene065582.t1

MATEVVLLDTWVSMFGMRVRIALAEKGVKYEYKEENLRNKSPLLLQMNPIHKKIPVLIHNGKPICESAIIVQYIDEVWNDKAPIMPSDPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEEGKKELISIFKTLEETLGDKPFYGGTTFGFLDIGLIPFYSWFYAYETYGNFKMEVECPKLMAWAKRCLEKESVSKTLPDQMKVYDFVVSMKKTLGLD