AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068679

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068679.t1 MTR_2g070200 97.285 221 5 1 1 220 1 221 1.26e-159 440
MS.gene068679.t1 MTR_2g070150 75.610 205 49 1 1 205 1 204 2.39e-118 335
MS.gene068679.t1 MTR_2g070210 70.721 222 62 2 1 220 1 221 3.01e-116 330
MS.gene068679.t1 MTR_2g070180 69.725 218 64 2 1 218 1 216 1.83e-114 326
MS.gene068679.t1 MTR_2g070140 72.549 204 55 1 17 220 1 203 5.15e-112 319
MS.gene068679.t1 MTR_2g070130 65.000 220 76 1 1 220 1 219 9.21e-109 311
MS.gene068679.t1 MTR_2g070060 59.817 219 87 1 1 219 1 218 1.31e-98 286
MS.gene068679.t1 MTR_2g070070 61.644 219 83 1 1 219 1 218 1.50e-97 283
MS.gene068679.t1 MTR_3g467420 61.574 216 82 1 5 220 10 224 2.29e-95 278
MS.gene068679.t1 MTR_8g056940 60.369 217 85 1 1 217 1 216 5.29e-93 271
MS.gene068679.t1 MTR_3g467430 62.802 207 76 1 3 209 8 213 1.67e-91 268
MS.gene068679.t1 MTR_4g124130 58.636 220 89 2 1 219 1 219 1.15e-90 266
MS.gene068679.t1 MTR_2g070110 57.534 219 91 1 1 219 1 217 5.86e-90 264
MS.gene068679.t1 MTR_2g070120 60.476 210 82 1 1 210 1 209 5.34e-89 261
MS.gene068679.t1 MTR_5g076900 48.182 220 111 3 2 220 4 221 8.00e-69 210
MS.gene068679.t1 MTR_0186s0030 61.972 142 53 1 1 142 1 141 5.63e-58 180
MS.gene068679.t1 MTR_1g090060 42.202 218 120 4 4 217 3 218 4.73e-52 167
MS.gene068679.t1 MTR_4g059730 42.081 221 120 5 1 216 1 218 1.30e-51 166
MS.gene068679.t1 MTR_8g087425 42.512 207 115 3 2 206 3 207 1.79e-50 164
MS.gene068679.t1 MTR_1g090070 39.726 219 125 5 4 217 9 225 1.46e-49 161
MS.gene068679.t1 MTR_7g065720 41.905 210 117 4 3 209 6 213 1.48e-49 161
MS.gene068679.t1 MTR_5g040430 37.736 212 124 3 2 206 5 215 2.86e-49 160
MS.gene068679.t1 MTR_5g037380 40.741 216 120 3 2 209 3 218 1.22e-48 159
MS.gene068679.t1 MTR_7g065660 41.429 210 119 3 3 210 6 213 4.20e-48 157
MS.gene068679.t1 MTR_7g065740 42.180 211 115 5 3 209 6 213 1.35e-47 156
MS.gene068679.t1 MTR_3g099757 42.788 208 115 4 3 208 4 209 3.93e-47 155
MS.gene068679.t1 MTR_4g019780 39.631 217 125 4 3 216 7 220 1.75e-46 153
MS.gene068679.t1 MTR_8g087410 42.000 200 112 3 4 201 6 203 7.48e-46 152
MS.gene068679.t1 MTR_1g090100 42.105 209 115 4 5 209 3 209 7.85e-46 152
MS.gene068679.t1 MTR_7g065750 41.584 202 113 4 3 201 6 205 9.15e-45 149
MS.gene068679.t1 MTR_7g065590 40.000 210 122 3 3 210 6 213 1.76e-44 148
MS.gene068679.t1 MTR_7g065600 41.294 201 114 3 3 201 6 204 1.94e-44 148
MS.gene068679.t1 MTR_1g090090 41.284 218 122 5 4 217 3 218 2.65e-44 148
MS.gene068679.t1 MTR_4g019790 39.423 208 122 3 4 209 7 212 7.72e-44 147
MS.gene068679.t1 MTR_7g065270 42.105 209 117 4 3 209 6 212 1.69e-43 145
MS.gene068679.t1 MTR_1g115195 41.706 211 115 6 1 206 1 208 1.97e-43 145
MS.gene068679.t1 MTR_7g065230 41.410 227 106 7 3 218 6 216 2.60e-43 145
MS.gene068679.t1 MTR_7g065265 42.105 209 117 4 3 209 6 212 6.64e-43 144
MS.gene068679.t1 MTR_1g090150 38.249 217 127 5 3 215 2 215 1.26e-42 144
MS.gene068679.t1 MTR_7g065290 42.857 210 114 6 3 209 6 212 1.45e-42 143
MS.gene068679.t1 MTR_6g080440 41.624 197 109 4 18 211 17 210 1.58e-42 143
MS.gene068679.t1 MTR_7g065710 44.848 165 87 3 1 163 1 163 5.30e-42 142
MS.gene068679.t1 MTR_7g065630 39.130 207 122 4 3 209 24 226 1.17e-41 141
MS.gene068679.t1 MTR_8g061950 39.252 214 118 5 3 206 4 215 1.82e-41 141
MS.gene068679.t1 MTR_7g065640 39.423 208 106 5 3 209 6 194 1.43e-39 135
MS.gene068679.t1 MTR_7g065700 38.756 209 124 4 1 209 4 208 4.53e-38 132
MS.gene068679.t1 MTR_7g065260 41.791 201 113 3 3 201 13 211 1.13e-37 131
MS.gene068679.t1 MTR_7g065680 44.444 162 86 3 3 162 6 165 8.58e-37 128
MS.gene068679.t1 MTR_2g072120 61.644 73 28 0 47 119 1 73 7.58e-31 108
MS.gene068679.t1 MTR_4g134380 30.588 170 102 4 4 160 15 181 2.25e-13 67.0
MS.gene068679.t1 MTR_4g134370 27.358 212 134 8 3 201 14 218 2.43e-12 64.3
MS.gene068679.t1 MTR_4g043640 57.143 42 18 0 149 190 44 85 3.77e-12 60.5
MS.gene068679.t1 MTR_4g134380 29.878 164 99 4 4 154 15 175 9.28e-12 61.6
MS.gene068679.t1 MTR_7g100320 28.571 203 131 8 11 208 33 226 1.14e-11 62.4
MS.gene068679.t1 MTR_1g067180 28.934 197 120 9 11 199 34 218 4.46e-11 60.8
MS.gene068679.t1 MTR_1g067180 28.934 197 120 9 11 199 31 215 4.91e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068679.t1 AT1G17180 63.182 220 80 1 1 220 1 219 2.98e-103 298
MS.gene068679.t1 AT1G78380 64.055 217 77 1 1 217 1 216 3.63e-101 292
MS.gene068679.t1 AT1G78340 63.636 209 75 1 1 209 1 208 8.54e-99 286
MS.gene068679.t1 AT1G17170 61.818 220 82 2 1 220 1 218 1.06e-97 283
MS.gene068679.t1 AT1G78370 61.395 215 82 1 1 215 1 214 1.02e-95 278
MS.gene068679.t1 AT1G78360 61.538 221 83 2 1 220 1 220 1.58e-95 278
MS.gene068679.t1 AT1G78320 60.000 220 86 2 1 219 1 219 4.94e-94 274
MS.gene068679.t1 AT1G53680 55.760 217 95 1 5 220 8 224 1.05e-87 258
MS.gene068679.t1 AT1G17190 55.556 216 95 1 3 218 4 218 3.57e-87 257
MS.gene068679.t1 AT3G43800 52.074 217 99 4 3 215 4 219 2.47e-78 234
MS.gene068679.t1 AT1G78320 51.818 220 77 3 1 219 1 192 2.06e-72 218
MS.gene068679.t1 AT2G29420 44.796 221 118 4 2 220 7 225 5.89e-58 183
MS.gene068679.t1 AT3G09270 42.995 207 114 3 3 206 5 210 3.50e-51 166
MS.gene068679.t1 AT1G10360 42.326 215 117 3 2 209 3 217 2.05e-50 164
MS.gene068679.t1 AT2G29490 39.810 211 118 6 3 208 6 212 2.31e-47 155
MS.gene068679.t1 AT1G59700 42.202 218 118 4 3 212 5 222 3.21e-47 155
MS.gene068679.t1 AT1G10370 41.395 215 119 3 2 209 3 217 8.11e-47 154
MS.gene068679.t1 AT2G29440 39.545 220 124 6 3 217 5 220 6.86e-46 152
MS.gene068679.t1 AT2G29480 39.336 211 119 6 3 208 6 212 2.87e-45 150
MS.gene068679.t1 AT1G27130 38.605 215 124 3 3 209 5 219 4.68e-45 150
MS.gene068679.t1 AT1G74590 37.000 200 119 3 3 196 6 204 3.80e-44 147
MS.gene068679.t1 AT2G29460 37.727 220 128 6 3 217 6 221 1.95e-43 145
MS.gene068679.t1 AT2G29450 39.409 203 117 4 3 201 5 205 2.29e-43 145
MS.gene068679.t1 AT1G27140 37.788 217 126 3 3 210 5 221 1.32e-42 144
MS.gene068679.t1 AT1G69930 38.389 211 124 3 5 209 13 223 2.22e-42 143
MS.gene068679.t1 AT1G59670 39.908 218 123 4 3 212 5 222 2.49e-42 143
MS.gene068679.t1 AT2G29470 36.986 219 128 7 5 217 8 222 8.37e-42 141
MS.gene068679.t1 AT5G62480 36.937 222 125 5 1 210 5 223 1.42e-41 141
MS.gene068679.t1 AT1G69920 34.529 223 135 4 5 219 35 254 3.69e-37 130
MS.gene068679.t1 AT5G62480 29.730 222 115 6 1 210 5 197 1.11e-23 94.4
MS.gene068679.t1 AT5G62480 29.730 222 114 6 1 210 5 196 1.27e-23 94.4
MS.gene068679.t1 AT5G02780 27.317 205 136 8 11 210 37 233 3.95e-11 61.2
MS.gene068679.t1 AT5G02780 27.317 205 136 8 11 210 37 233 3.95e-11 61.2
MS.gene068679.t1 AT5G02790 31.472 197 109 10 11 199 35 213 5.74e-11 60.5
MS.gene068679.t1 AT5G02780 27.317 205 134 8 11 210 37 231 7.06e-11 60.5

Find 59 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGAGACAAGGCTTATTT+TGG 0.162898 2.2:+21555369 MS.gene068679:CDS
AGAGAGCACAAGCTAGATTT+TGG 0.186476 2.2:+21554570 MS.gene068679:CDS
ACTTAATGCCTAATTTCTTT+AGG 0.190935 2.2:-21555590 None:intergenic
CTTAATGCCTAATTTCTTTA+GGG 0.237574 2.2:-21555589 None:intergenic
GGTTTCTGGGTAAGTCCTTA+TGG 0.256251 2.2:+21554323 MS.gene068679:CDS
CATATCTATGAATTCCTTCT+TGG 0.269924 2.2:-21555325 None:intergenic
TTTGAAACATATGAGAAGTT+TGG 0.284132 2.2:+21555444 MS.gene068679:CDS
ATTACAAGTGACTCACAAAT+AGG 0.297067 2.2:-21554485 None:intergenic
GTTTCTGGGTAAGTCCTTAT+GGG 0.344090 2.2:+21554324 MS.gene068679:CDS
CATAGATATGCTCAAATTGT+TGG 0.364566 2.2:+21555338 MS.gene068679:CDS
AAGCTGGAAGGAAAGTTTAT+AGG 0.375912 2.2:+21555274 MS.gene068679:CDS
GTGCCCTAAGTTCATTGCTT+GGG 0.394706 2.2:+21555488 MS.gene068679:CDS
AAGAATAGCCCTTGCTGAAA+AGG 0.396907 2.2:+21554355 MS.gene068679:CDS
ATAAAGATCCAAGGGAGAAT+TGG 0.398339 2.2:+21554399 MS.gene068679:CDS
GCAGATGCCCTAAAGAAATT+AGG 0.402688 2.2:+21555582 MS.gene068679:CDS
TTGCCATTGTGGATTAGAAC+CGG 0.405758 2.2:-21554461 None:intergenic
CACAAATAGGTTTGCCATTG+TGG 0.406278 2.2:-21554472 None:intergenic
AAAATCTAGCTTGTGCTCTC+TGG 0.411980 2.2:-21554568 None:intergenic
TCGTAGACCTTATGCTGGTT+AGG 0.419263 2.2:-21555552 None:intergenic
TTGAGTATATTGATGAGGTT+TGG 0.428745 2.2:+21554510 MS.gene068679:CDS
AGTGCCCTAAGTTCATTGCT+TGG 0.433594 2.2:+21555487 MS.gene068679:CDS
TTAGCCCAAGCAATGAACTT+AGG 0.435179 2.2:-21555492 None:intergenic
TGGAGGAGACAGACTTGGTT+TGG 0.440850 2.2:+21555389 MS.gene068679:CDS
GGCACTCAATCTCTATATTG+AGG 0.444351 2.2:-21555470 None:intergenic
GTTTCAAACCAACTGTAGAA+TGG 0.455904 2.2:-21555429 None:intergenic
TTTCAAACCAACTGTAGAAT+GGG 0.460044 2.2:-21555428 None:intergenic
GGCTGACTATGTTGATAAGA+AGG 0.461602 2.2:+21554592 MS.gene068679:CDS
CAAATTGTTGGAGAAACAGC+TGG 0.465628 2.2:+21555350 MS.gene068679:CDS
TAGCCCAAGCAATGAACTTA+GGG 0.471249 2.2:-21555491 None:intergenic
CCCTAGTCCCATTCTACAGT+TGG 0.473896 2.2:+21555421 MS.gene068679:CDS
GTCTCTTCCTAACCAGCATA+AGG 0.491160 2.2:+21555545 MS.gene068679:CDS
AGTTCATTGCTTGGGCTAAG+AGG 0.495968 2.2:+21555496 MS.gene068679:CDS
AGAATAGCCCTTGCTGAAAA+GGG 0.508196 2.2:+21554356 MS.gene068679:CDS
ATTCTTGCTCTCATCCCATA+AGG 0.517094 2.2:-21554338 None:intergenic
CTAGCTTGTGCTCTCTGGTA+GGG 0.530129 2.2:-21554563 None:intergenic
AGAACAAGAAGCTGCCAAGA+AGG 0.534444 2.2:+21555311 MS.gene068679:CDS
AGATGAGGCCAATTCTCCCT+TGG 0.542925 2.2:-21554407 None:intergenic
TGTCAATAGATATATGAAGC+TGG 0.543339 2.2:+21555258 MS.gene068679:intron
GTATATTGATGAGGTTTGGA+AGG 0.548215 2.2:+21554514 MS.gene068679:CDS
TGCCATTGTGGATTAGAACC+GGG 0.551246 2.2:-21554460 None:intergenic
TCTAGCTTGTGCTCTCTGGT+AGG 0.552026 2.2:-21554564 None:intergenic
CTAAGTCATGGCTGATGAGG+TGG 0.569512 2.2:+21554292 None:intergenic
CTACTAAGTCATGGCTGATG+AGG 0.574341 2.2:+21554289 None:intergenic
CCCGGTTCACAAGAAAATCC+CGG 0.589293 2.2:+21554442 MS.gene068679:CDS
GGAGAAACAGCTGGGAGACA+AGG 0.589733 2.2:+21555359 MS.gene068679:CDS
AATAGATATATGAAGCTGGA+AGG 0.589985 2.2:+21555262 MS.gene068679:intron
CCAACTGTAGAATGGGACTA+GGG 0.601552 2.2:-21555421 None:intergenic
GCTCTCTGGTAGGGATCAGA+AGG 0.603013 2.2:-21554554 None:intergenic
ATGCTGGTTAGGAAGAGACT+TGG 0.605348 2.2:-21555541 None:intergenic
GGGTTCATTTGTAGAAGATG+AGG 0.610571 2.2:-21554422 None:intergenic
ACCAACTGTAGAATGGGACT+AGG 0.615269 2.2:-21555422 None:intergenic
AGTTCATAGATAAAGATCCA+AGG 0.616881 2.2:+21554390 MS.gene068679:CDS
GTTCATAGATAAAGATCCAA+GGG 0.618886 2.2:+21554391 MS.gene068679:CDS
TGAACTCGTAGACCTTATGC+TGG 0.626524 2.2:-21555557 None:intergenic
AAATTGTTGGAGAAACAGCT+GGG 0.647590 2.2:+21555351 MS.gene068679:CDS
AATTGTTGAGTATATTGATG+AGG 0.670411 2.2:+21554505 MS.gene068679:CDS
TCATCTTCTACAAATGAACC+CGG 0.681998 2.2:+21554424 MS.gene068679:CDS
TAGGGATCAGAAGGCAACAA+CGG 0.683708 2.2:-21554545 None:intergenic
ATCCCGGTTCTAATCCACAA+TGG 0.688019 2.2:+21554458 MS.gene068679:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAACAAATAATAAATAT+TGG - chr2.2:21554864-21554883 None:intergenic 10.0%
!! AACAAATAATAAATATTGGA+TGG - chr2.2:21554860-21554879 None:intergenic 15.0%
!! GGATTAAAAATTTATTGAAT+GGG + chr2.2:21555029-21555048 MS.gene068679:intron 15.0%
!! TGGATTAAAAATTTATTGAA+TGG + chr2.2:21555028-21555047 MS.gene068679:intron 15.0%
!!! AATAAATTTTTAATCCAACT+AGG - chr2.2:21555025-21555044 None:intergenic 15.0%
!!! TTTCTTTAATTTTTTTAGAG+AGG + chr2.2:21554878-21554897 MS.gene068679:intron 15.0%
!! AATTTCACTAAAAGTTGTTA+AGG + chr2.2:21555160-21555179 MS.gene068679:intron 20.0%
!! GTCAAACAAGTAAAATTTAA+AGG - chr2.2:21554631-21554650 None:intergenic 20.0%
!!! ATATTTTTATCAGTTGAGTT+AGG + chr2.2:21554758-21554777 MS.gene068679:intron 20.0%
!!! TTAATTTTTTTAGAGAGGAA+GGG + chr2.2:21554883-21554902 MS.gene068679:intron 20.0%
!!! TTTAATTTTTTTAGAGAGGA+AGG + chr2.2:21554882-21554901 MS.gene068679:intron 20.0%
!!! TTTTGTTTCCAACTAATTTT+GGG + chr2.2:21555222-21555241 MS.gene068679:intron 20.0%
!!! TTTTTGTTTCCAACTAATTT+TGG + chr2.2:21555221-21555240 MS.gene068679:intron 20.0%
! AAAGTTTATAGGACCAAAAA+AGG + chr2.2:21555285-21555304 MS.gene068679:CDS 25.0%
! AATGTAATTTGATACTGAGT+AGG - chr2.2:21555066-21555085 None:intergenic 25.0%
! AATTAACACCCAAAATTAGT+TGG - chr2.2:21555233-21555252 None:intergenic 25.0%
! AATTGTTGAGTATATTGATG+AGG + chr2.2:21554505-21554524 MS.gene068679:CDS 25.0%
! ACTTAATGCCTAATTTCTTT+AGG - chr2.2:21555593-21555612 None:intergenic 25.0%
! CTTAATGCCTAATTTCTTTA+GGG - chr2.2:21555592-21555611 None:intergenic 25.0%
! GTAGGCAAAAATAAGTTAAT+TGG - chr2.2:21554987-21555006 None:intergenic 25.0%
! TACTCAGTATCAAATTACAT+TGG + chr2.2:21555065-21555084 MS.gene068679:intron 25.0%
!! CTTTCATCAATCTCTATTTT+AGG + chr2.2:21554822-21554841 MS.gene068679:intron 25.0%
!! TATTTTACAAAACTCCTAGT+TGG + chr2.2:21555008-21555027 MS.gene068679:intron 25.0%
!! TTTGAAACATATGAGAAGTT+TGG + chr2.2:21555444-21555463 MS.gene068679:CDS 25.0%
!!! AGGAGTTTTGTAAAATATGT+AGG - chr2.2:21555005-21555024 None:intergenic 25.0%
!!! TCAATCTCTATTTTAGGTTT+TGG + chr2.2:21554828-21554847 MS.gene068679:intron 25.0%
AATAGATATATGAAGCTGGA+AGG + chr2.2:21555262-21555281 MS.gene068679:intron 30.0%
AGTTCATAGATAAAGATCCA+AGG + chr2.2:21554390-21554409 MS.gene068679:CDS 30.0%
ATTACAAGTGACTCACAAAT+AGG - chr2.2:21554488-21554507 None:intergenic 30.0%
CATAGATATGCTCAAATTGT+TGG + chr2.2:21555338-21555357 MS.gene068679:CDS 30.0%
CATATCTATGAATTCCTTCT+TGG - chr2.2:21555328-21555347 None:intergenic 30.0%
GTTCATAGATAAAGATCCAA+GGG + chr2.2:21554391-21554410 MS.gene068679:CDS 30.0%
TGTCAATAGATATATGAAGC+TGG + chr2.2:21555258-21555277 MS.gene068679:intron 30.0%
TTGAGTATATTGATGAGGTT+TGG + chr2.2:21554510-21554529 MS.gene068679:CDS 30.0%
TTTCAAACCAACTGTAGAAT+GGG - chr2.2:21555431-21555450 None:intergenic 30.0%
!!! TTCTTGTTCTTCTCCTTTTT+TGG - chr2.2:21555301-21555320 None:intergenic 30.0%
AAATTGTTGGAGAAACAGCT+GGG + chr2.2:21555351-21555370 MS.gene068679:CDS 35.0%
AAGCTGGAAGGAAAGTTTAT+AGG + chr2.2:21555274-21555293 MS.gene068679:CDS 35.0%
ATAAAGATCCAAGGGAGAAT+TGG + chr2.2:21554399-21554418 MS.gene068679:CDS 35.0%
GAGAAGACATCAAACTACAT+TGG + chr2.2:21554939-21554958 MS.gene068679:intron 35.0%
GTATATTGATGAGGTTTGGA+AGG + chr2.2:21554514-21554533 MS.gene068679:CDS 35.0%
GTTTCAAACCAACTGTAGAA+TGG - chr2.2:21555432-21555451 None:intergenic 35.0%
TCATCTTCTACAAATGAACC+CGG + chr2.2:21554424-21554443 MS.gene068679:CDS 35.0%
!!! CTCTATTTTAGGTTTTGGTG+TGG + chr2.2:21554833-21554852 MS.gene068679:intron 35.0%
AAGAATAGCCCTTGCTGAAA+AGG + chr2.2:21554355-21554374 MS.gene068679:CDS 40.0%
AGAATAGCCCTTGCTGAAAA+GGG + chr2.2:21554356-21554375 MS.gene068679:CDS 40.0%
ATTCTTGCTCTCATCCCATA+AGG - chr2.2:21554341-21554360 None:intergenic 40.0%
CAAATTGTTGGAGAAACAGC+TGG + chr2.2:21555350-21555369 MS.gene068679:CDS 40.0%
CACAAATAGGTTTGCCATTG+TGG - chr2.2:21554475-21554494 None:intergenic 40.0%
GCAGATGCCCTAAAGAAATT+AGG + chr2.2:21555582-21555601 MS.gene068679:CDS 40.0%
GGCACTCAATCTCTATATTG+AGG - chr2.2:21555473-21555492 None:intergenic 40.0%
GGGTTCATTTGTAGAAGATG+AGG - chr2.2:21554425-21554444 None:intergenic 40.0%
TAGCCCAAGCAATGAACTTA+GGG - chr2.2:21555494-21555513 None:intergenic 40.0%
TTAGAACTGACACACACAAG+TGG + chr2.2:21554687-21554706 MS.gene068679:intron 40.0%
TTAGCCCAAGCAATGAACTT+AGG - chr2.2:21555495-21555514 None:intergenic 40.0%
! AAAATCTAGCTTGTGCTCTC+TGG - chr2.2:21554571-21554590 None:intergenic 40.0%
! GGCTGACTATGTTGATAAGA+AGG + chr2.2:21554592-21554611 MS.gene068679:CDS 40.0%
! GTTTCTGGGTAAGTCCTTAT+GGG + chr2.2:21554324-21554343 MS.gene068679:CDS 40.0%
!! AGAGAGCACAAGCTAGATTT+TGG + chr2.2:21554570-21554589 MS.gene068679:CDS 40.0%
!! GAGAGCACAAGCTAGATTTT+GGG + chr2.2:21554571-21554590 MS.gene068679:CDS 40.0%
!! TATTTTGGAGGAGACAGACT+TGG + chr2.2:21555384-21555403 MS.gene068679:CDS 40.0%
!! TTGCCATTGTGGATTAGAAC+CGG - chr2.2:21554464-21554483 None:intergenic 40.0%
AACTGACACACACAAGTGGA+GGG + chr2.2:21554691-21554710 MS.gene068679:intron 45.0%
ACCAACTGTAGAATGGGACT+AGG - chr2.2:21555425-21555444 None:intergenic 45.0%
AGAACAAGAAGCTGCCAAGA+AGG + chr2.2:21555311-21555330 MS.gene068679:CDS 45.0%
AGTGCCCTAAGTTCATTGCT+TGG + chr2.2:21555487-21555506 MS.gene068679:CDS 45.0%
AGTTCATTGCTTGGGCTAAG+AGG + chr2.2:21555496-21555515 MS.gene068679:CDS 45.0%
ATAAGTCAGACGCTTTGACC+AGG - chr2.2:21554731-21554750 None:intergenic 45.0%
CCAACTGTAGAATGGGACTA+GGG - chr2.2:21555424-21555443 None:intergenic 45.0%
GTCTCTTCCTAACCAGCATA+AGG + chr2.2:21555545-21555564 MS.gene068679:CDS 45.0%
GTGCCCTAAGTTCATTGCTT+GGG + chr2.2:21555488-21555507 MS.gene068679:CDS 45.0%
TAAGTCAGACGCTTTGACCA+GGG - chr2.2:21554730-21554749 None:intergenic 45.0%
TAGGGATCAGAAGGCAACAA+CGG - chr2.2:21554548-21554567 None:intergenic 45.0%
TGAACTCGTAGACCTTATGC+TGG - chr2.2:21555560-21555579 None:intergenic 45.0%
! ACCGGGATTTTCTTGTGAAC+CGG - chr2.2:21554446-21554465 None:intergenic 45.0%
! ACTTGACACCCTTTTCAGCA+AGG - chr2.2:21554367-21554386 None:intergenic 45.0%
! ATCCCGGTTCTAATCCACAA+TGG + chr2.2:21554458-21554477 MS.gene068679:CDS 45.0%
! ATGCTGGTTAGGAAGAGACT+TGG - chr2.2:21555544-21555563 None:intergenic 45.0%
! CTGGGAGACAAGGCTTATTT+TGG + chr2.2:21555369-21555388 MS.gene068679:CDS 45.0%
! CTTGACACCCTTTTCAGCAA+GGG - chr2.2:21554366-21554385 None:intergenic 45.0%
! GGTTTCTGGGTAAGTCCTTA+TGG + chr2.2:21554323-21554342 MS.gene068679:CDS 45.0%
! TCGTAGACCTTATGCTGGTT+AGG - chr2.2:21555555-21555574 None:intergenic 45.0%
!! AGGTGGTTTTGCTAGGTTTC+TGG + chr2.2:21554309-21554328 MS.gene068679:CDS 45.0%
!! GGTGGTTTTGCTAGGTTTCT+GGG + chr2.2:21554310-21554329 MS.gene068679:CDS 45.0%
!! TGCCATTGTGGATTAGAACC+GGG - chr2.2:21554463-21554482 None:intergenic 45.0%
!!! GGAGACAAGGCTTATTTTGG+AGG + chr2.2:21555372-21555391 MS.gene068679:CDS 45.0%
AGATGAGGCCAATTCTCCCT+TGG - chr2.2:21554410-21554429 None:intergenic 50.0%
CCCGGTTCACAAGAAAATCC+CGG + chr2.2:21554442-21554461 MS.gene068679:CDS 50.0%
CCCTAGTCCCATTCTACAGT+TGG + chr2.2:21555421-21555440 MS.gene068679:CDS 50.0%
GAACTGACACACACAAGTGG+AGG + chr2.2:21554690-21554709 MS.gene068679:intron 50.0%
! CCGGGATTTTCTTGTGAACC+GGG - chr2.2:21554445-21554464 None:intergenic 50.0%
! CTAGCTTGTGCTCTCTGGTA+GGG - chr2.2:21554566-21554585 None:intergenic 50.0%
! TCTAGCTTGTGCTCTCTGGT+AGG - chr2.2:21554567-21554586 None:intergenic 50.0%
! TGGAGGAGACAGACTTGGTT+TGG + chr2.2:21555389-21555408 MS.gene068679:CDS 50.0%
!! GCTGATGAGGTGGTTTTGCT+AGG + chr2.2:21554302-21554321 MS.gene068679:CDS 50.0%
ACACACAAGTGGAGGGACGA+GGG + chr2.2:21554698-21554717 MS.gene068679:intron 55.0%
GCTCTCTGGTAGGGATCAGA+AGG - chr2.2:21554557-21554576 None:intergenic 55.0%
GGAGAAACAGCTGGGAGACA+AGG + chr2.2:21555359-21555378 MS.gene068679:CDS 55.0%
CACACACAAGTGGAGGGACG+AGG + chr2.2:21554697-21554716 MS.gene068679:intron 60.0%
AGGGACGAGGGTTCGAACCC+TGG + chr2.2:21554710-21554729 MS.gene068679:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 21554299 21555614 21554299 ID=MS.gene068679
chr2.2 mRNA 21554299 21555614 21554299 ID=MS.gene068679.t1;Parent=MS.gene068679
chr2.2 exon 21554299 21554613 21554299 ID=MS.gene068679.t1.exon1;Parent=MS.gene068679.t1
chr2.2 CDS 21554299 21554613 21554299 ID=cds.MS.gene068679.t1;Parent=MS.gene068679.t1
chr2.2 exon 21555267 21555614 21555267 ID=MS.gene068679.t1.exon2;Parent=MS.gene068679.t1
chr2.2 CDS 21555267 21555614 21555267 ID=cds.MS.gene068679.t1;Parent=MS.gene068679.t1
Gene Sequence

>MS.gene068679

ATGGCTGATGAGGTGGTTTTGCTAGGTTTCTGGGTAAGTCCTTATGGGATGAGAGCAAGAATAGCCCTTGCTGAAAAGGGTGTCAAGTATGAGTTCATAGATAAAGATCCAAGGGAGAATTGGCCTCATCTTCTACAAATGAACCCGGTTCACAAGAAAATCCCGGTTCTAATCCACAATGGCAAACCTATTTGTGAGTCACTTGTAATTGTTGAGTATATTGATGAGGTTTGGAAGGATAGATCTCCGTTGTTGCCTTCTGATCCCTACCAGAGAGCACAAGCTAGATTTTGGGCTGACTATGTTGATAAGAAGGTATATAATAAAAGCCTTTAAATTTTACTTGTTTGACTCATATTTTTTAGATAGACGTAAAGACAAATAACTATTAGAACTGACACACACAAGTGGAGGGACGAGGGTTCGAACCCTGGTCAAAGCGTCTGACTTATAATTTTGATATTTTTATCAGTTGAGTTAGGACTTGTAAACTGACACGTATATTAAATCTTGCTAAATTATGCTTTCATCAATCTCTATTTTAGGTTTTGGTGTGGTCCATCCAATATTTATTATTTGTTTCTTTAATTTTTTTAGAGAGGAAGGGAATTTATTACTATTTTTAATGAAAATAGTTTATGAGAAGACATCAAACTACATTGGTGTAACTTAATAGTTACACTGTCCAATTAACTTATTTTTGCCTACATATTTTACAAAACTCCTAGTTGGATTAAAAATTTATTGAATGGGTCTAATATGAACCTACTCAGTATCAAATTACATTGGTGTAAACATTTTTAAAAGTTCTGATGCAAAAGTTTGTACATCATCAGTATTTTTTTGTATTATCAAATCTACAATTTCACTAAAAGTTGTTAAGGTCGATCTTTGACTCTTATGTACGTTTCTCATTCTTGCGTTTTTGTTTCCAACTAATTTTGGGTGTTAATTATCAATGTCAATAGATATATGAAGCTGGAAGGAAAGTTTATAGGACCAAAAAAGGAGAAGAACAAGAAGCTGCCAAGAAGGAATTCATAGATATGCTCAAATTGTTGGAGAAACAGCTGGGAGACAAGGCTTATTTTGGAGGAGACAGACTTGGTTTGGTAGATATAGCCCTAGTCCCATTCTACAGTTGGTTTGAAACATATGAGAAGTTTGGAAACCTCAATATAGAGATTGAGTGCCCTAAGTTCATTGCTTGGGCTAAGAGGTGCATGCAGATTGAGAGTGTTTCCAAGTCTCTTCCTAACCAGCATAAGGTCTACGAGTTCATTGCAGATGCCCTAAAGAAATTAGGCATTAAGTAG
Protein sequence

>MS.gene068679.t1

MADEVVLLGFWVSPYGMRARIALAEKGVKYEFIDKDPRENWPHLLQMNPVHKKIPVLIHNGKPICESLVIVEYIDEVWKDRSPLLPSDPYQRAQARFWADYVDKKIYEAGRKVYRTKKGEEQEAAKKEFIDMLKLLEKQLGDKAYFGGDRLGLVDIALVPFYSWFETYEKFGNLNIEIECPKFIAWAKRCMQIESVSKSLPNQHKVYEFIADALKKLGIK