AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062845

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062845.t1 MTR_2g070200 96.380 221 7 1 1 220 1 221 3.42e-157 434
MS.gene062845.t1 MTR_2g070150 74.146 205 52 1 1 205 1 204 2.90e-117 332
MS.gene062845.t1 MTR_2g070210 69.820 222 64 2 1 220 1 221 1.89e-114 326
MS.gene062845.t1 MTR_2g070180 68.349 218 67 2 1 218 1 216 3.86e-113 322
MS.gene062845.t1 MTR_2g070140 71.569 204 57 1 17 220 1 203 2.47e-111 317
MS.gene062845.t1 MTR_2g070130 64.091 220 78 1 1 220 1 219 6.19e-108 309
MS.gene062845.t1 MTR_2g070060 59.361 219 88 1 1 219 1 218 2.86e-97 282
MS.gene062845.t1 MTR_2g070070 61.187 219 84 1 1 219 1 218 8.45e-97 281
MS.gene062845.t1 MTR_3g467420 62.037 216 81 1 5 220 10 224 3.45e-96 280
MS.gene062845.t1 MTR_3g467430 63.285 207 75 1 3 209 8 213 5.17e-92 269
MS.gene062845.t1 MTR_8g056940 59.447 217 87 1 1 217 1 216 8.52e-92 268
MS.gene062845.t1 MTR_4g124130 57.727 220 91 2 1 219 1 219 8.61e-90 263
MS.gene062845.t1 MTR_2g070110 57.078 219 92 1 1 219 1 217 4.89e-89 261
MS.gene062845.t1 MTR_2g070120 59.524 210 84 1 1 210 1 209 6.75e-88 258
MS.gene062845.t1 MTR_5g076900 47.727 220 112 3 2 220 4 221 6.71e-68 208
MS.gene062845.t1 MTR_0186s0030 60.563 142 55 1 1 142 1 141 6.34e-57 177
MS.gene062845.t1 MTR_1g090060 42.202 218 120 4 4 217 3 218 1.74e-52 169
MS.gene062845.t1 MTR_4g059730 42.081 221 120 5 1 216 1 218 1.52e-51 166
MS.gene062845.t1 MTR_1g090070 39.726 219 125 5 4 217 9 225 6.84e-50 162
MS.gene062845.t1 MTR_8g087425 41.546 207 117 3 2 206 3 207 1.17e-49 161
MS.gene062845.t1 MTR_7g065720 40.952 210 119 4 3 209 6 213 1.22e-48 159
MS.gene062845.t1 MTR_5g040430 36.792 212 126 3 2 206 5 215 8.04e-48 157
MS.gene062845.t1 MTR_5g037380 40.278 216 121 3 2 209 3 218 1.02e-47 157
MS.gene062845.t1 MTR_7g065740 40.758 211 118 5 3 209 6 213 1.09e-46 154
MS.gene062845.t1 MTR_7g065660 40.476 210 121 3 3 210 6 213 2.46e-46 153
MS.gene062845.t1 MTR_1g090100 42.105 209 115 4 5 209 3 209 3.60e-46 152
MS.gene062845.t1 MTR_3g099757 42.308 208 116 4 3 208 4 209 5.22e-46 152
MS.gene062845.t1 MTR_8g087410 38.605 215 128 3 4 216 6 218 7.01e-45 149
MS.gene062845.t1 MTR_4g019780 39.171 217 126 4 3 216 7 220 7.89e-45 149
MS.gene062845.t1 MTR_1g090090 41.284 218 122 5 4 217 3 218 3.29e-44 147
MS.gene062845.t1 MTR_7g065590 39.524 210 123 3 3 210 6 213 1.18e-43 146
MS.gene062845.t1 MTR_7g065600 40.796 201 115 3 3 201 6 204 1.30e-43 146
MS.gene062845.t1 MTR_7g065750 40.594 202 115 4 3 201 6 205 1.45e-43 146
MS.gene062845.t1 MTR_1g115195 41.706 211 115 6 1 206 1 208 2.21e-43 145
MS.gene062845.t1 MTR_4g019790 40.000 200 116 3 4 201 7 204 3.90e-43 145
MS.gene062845.t1 MTR_1g090150 38.532 218 125 6 3 215 2 215 2.11e-42 143
MS.gene062845.t1 MTR_7g065265 41.627 209 118 4 3 209 6 212 2.82e-42 142
MS.gene062845.t1 MTR_6g080440 41.919 198 107 5 18 211 17 210 3.79e-42 142
MS.gene062845.t1 MTR_7g065270 41.148 209 119 4 3 209 6 212 4.59e-42 142
MS.gene062845.t1 MTR_7g065230 40.807 223 113 5 3 218 6 216 7.15e-42 141
MS.gene062845.t1 MTR_7g065290 41.148 209 119 4 3 209 6 212 6.30e-41 139
MS.gene062845.t1 MTR_7g065710 38.647 207 123 4 1 207 1 203 6.68e-41 139
MS.gene062845.t1 MTR_7g065630 39.423 208 120 6 3 209 24 226 9.86e-41 139
MS.gene062845.t1 MTR_8g061950 38.498 213 121 4 3 206 4 215 3.37e-40 137
MS.gene062845.t1 MTR_7g065640 38.462 208 108 5 3 209 6 194 2.16e-38 132
MS.gene062845.t1 MTR_7g065260 41.294 201 114 3 3 201 13 211 1.40e-36 128
MS.gene062845.t1 MTR_7g065700 37.799 209 126 4 1 209 4 208 3.15e-36 127
MS.gene062845.t1 MTR_7g065680 38.647 207 119 5 3 207 6 206 4.39e-36 127
MS.gene062845.t1 MTR_2g072120 60.274 73 29 0 47 119 1 73 1.43e-29 105
MS.gene062845.t1 MTR_4g134380 30.588 170 102 4 4 160 15 181 8.07e-13 65.5
MS.gene062845.t1 MTR_4g043640 57.143 42 18 0 149 190 44 85 3.70e-12 60.5
MS.gene062845.t1 MTR_7g100320 29.851 201 127 9 11 206 33 224 1.31e-11 62.4
MS.gene062845.t1 MTR_4g134370 26.887 212 135 7 3 201 14 218 1.49e-11 62.0
MS.gene062845.t1 MTR_1g067180 29.897 194 116 9 14 199 37 218 2.02e-11 62.0
MS.gene062845.t1 MTR_1g067180 29.442 197 119 9 11 199 31 215 2.53e-11 61.6
MS.gene062845.t1 MTR_4g134380 29.878 164 99 4 4 154 15 175 3.99e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062845.t1 AT1G78380 64.055 217 77 1 1 217 1 216 1.60e-102 296
MS.gene062845.t1 AT1G17180 62.273 220 82 1 1 220 1 219 4.03e-102 295
MS.gene062845.t1 AT1G78340 63.158 209 76 1 1 209 1 208 2.64e-98 285
MS.gene062845.t1 AT1G17170 61.364 220 83 2 1 220 1 218 6.51e-97 281
MS.gene062845.t1 AT1G78370 60.930 215 83 1 1 215 1 214 7.22e-96 279
MS.gene062845.t1 AT1G78360 61.086 221 84 2 1 220 1 220 5.31e-95 276
MS.gene062845.t1 AT1G78320 59.070 215 87 1 1 215 1 214 1.25e-92 271
MS.gene062845.t1 AT1G53680 56.221 217 94 1 5 220 8 224 6.06e-89 261
MS.gene062845.t1 AT1G17190 54.167 216 98 1 3 218 4 218 4.96e-84 249
MS.gene062845.t1 AT3G43800 51.152 217 101 4 3 215 4 219 8.98e-77 231
MS.gene062845.t1 AT1G78320 50.698 215 78 2 1 215 1 187 6.69e-71 214
MS.gene062845.t1 AT2G29420 44.796 221 118 4 2 220 7 225 1.27e-57 182
MS.gene062845.t1 AT3G09270 42.512 207 115 3 3 206 5 210 2.66e-50 163
MS.gene062845.t1 AT1G10360 42.326 215 117 3 2 209 3 217 5.87e-50 162
MS.gene062845.t1 AT2G29490 39.810 211 118 6 3 208 6 212 3.61e-47 155
MS.gene062845.t1 AT1G59700 41.743 218 119 4 3 212 5 222 2.53e-46 153
MS.gene062845.t1 AT1G10370 40.930 215 120 3 2 209 3 217 8.48e-46 152
MS.gene062845.t1 AT2G29480 39.810 211 118 6 3 208 6 212 3.56e-45 150
MS.gene062845.t1 AT2G29440 38.636 220 126 6 3 217 5 220 6.30e-45 149
MS.gene062845.t1 AT1G74590 37.981 208 121 4 3 204 6 211 4.98e-44 147
MS.gene062845.t1 AT2G29450 38.208 212 125 4 3 210 5 214 2.19e-43 145
MS.gene062845.t1 AT1G27130 37.674 215 126 3 3 209 5 219 2.38e-43 145
MS.gene062845.t1 AT2G29460 37.727 220 128 6 3 217 6 221 2.42e-43 145
MS.gene062845.t1 AT5G62480 36.937 222 125 5 1 210 5 223 1.48e-41 141
MS.gene062845.t1 AT1G69930 38.389 211 124 3 5 209 13 223 1.67e-41 141
MS.gene062845.t1 AT2G29470 36.986 219 128 7 5 217 8 222 1.84e-41 140
MS.gene062845.t1 AT1G59670 39.450 218 124 4 3 212 5 222 2.26e-41 140
MS.gene062845.t1 AT1G27140 36.866 217 128 3 3 210 5 221 3.56e-41 140
MS.gene062845.t1 AT1G69920 34.081 223 136 4 5 219 35 254 5.58e-36 127
MS.gene062845.t1 AT5G62480 29.730 222 115 6 1 210 5 197 1.05e-23 94.7
MS.gene062845.t1 AT5G62480 29.730 222 114 6 1 210 5 196 1.34e-23 94.4
MS.gene062845.t1 AT5G02780 27.941 204 134 8 11 209 37 232 2.18e-11 62.0
MS.gene062845.t1 AT5G02780 27.941 204 134 8 11 209 37 232 2.18e-11 62.0
MS.gene062845.t1 AT5G02780 28.866 194 123 8 11 199 37 220 3.57e-11 61.2
MS.gene062845.t1 AT5G02790 31.472 197 109 10 11 199 35 213 3.86e-11 61.2

Find 57 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGAGACAAGGCTTATTT+TGG 0.162898 2.4:+25136567 MS.gene062845:CDS
TGAGAGCACAAGCTAGATTT+TGG 0.184304 2.4:+25135772 MS.gene062845:CDS
ACTTAATGCCTAATTTCTTT+AGG 0.190935 2.4:-25136788 None:intergenic
CTTAATGCCTAATTTCTTTA+GGG 0.237574 2.4:-25136787 None:intergenic
CATATCTATGAATTCCTTCT+TGG 0.269924 2.4:-25136523 None:intergenic
TTTGAAACATATGAGAAGTT+TGG 0.284132 2.4:+25136642 MS.gene062845:CDS
ATTACAAGTGACTCACAAAT+AGG 0.297067 2.4:-25135687 None:intergenic
ATAAAGATGCAAGGGATAAT+TGG 0.310757 2.4:+25135601 MS.gene062845:CDS
GGTTTCTGGATAAGTCCTTA+TGG 0.328976 2.4:+25135525 MS.gene062845:CDS
CATAGATATGCTCAAATTGT+TGG 0.364566 2.4:+25136536 MS.gene062845:CDS
AAGCTGGAAGGAAAGTTTAT+AGG 0.375912 2.4:+25136472 MS.gene062845:CDS
GTGCCCTAAGTTCATTGCTT+GGG 0.394706 2.4:+25136686 MS.gene062845:CDS
AAGAATAGCCCTTGCTGAAA+AGG 0.396907 2.4:+25135557 MS.gene062845:CDS
GCAGATGCCCTAAAGAAATT+AGG 0.402688 2.4:+25136780 MS.gene062845:CDS
TTGCCATTGTGGATTAGAAC+CGG 0.405758 2.4:-25135663 None:intergenic
CACAAATAGGTTTGCCATTG+TGG 0.406278 2.4:-25135674 None:intergenic
TCGTAGACCTTATGCTGGTT+AGG 0.419263 2.4:-25136750 None:intergenic
TTGAGTATATTGATGAGGTT+TGG 0.428745 2.4:+25135712 MS.gene062845:CDS
AGTGCCCTAAGTTCATTGCT+TGG 0.433594 2.4:+25136685 MS.gene062845:CDS
TTAGCCCAAGCAATGAACTT+AGG 0.435179 2.4:-25136690 None:intergenic
AAAATCTAGCTTGTGCTCTC+AGG 0.436530 2.4:-25135770 None:intergenic
TGGAGGAGACAGACTTGGTT+TGG 0.440850 2.4:+25136587 MS.gene062845:CDS
GGCACTCAATCTCTATATTG+AGG 0.444351 2.4:-25136668 None:intergenic
GTTTCAAACCAACTGTAGAA+TGG 0.455904 2.4:-25136627 None:intergenic
TTTCAAACCAACTGTAGAAT+GGG 0.460044 2.4:-25136626 None:intergenic
ATTCTTGCTCTCATTCCATA+AGG 0.463260 2.4:-25135540 None:intergenic
GGCTGACTATGTTGATAAAA+CGG 0.464640 2.4:+25135794 MS.gene062845:CDS
CAAATTGTTGGAGAAACAGC+TGG 0.465628 2.4:+25136548 MS.gene062845:CDS
TAGCCCAAGCAATGAACTTA+GGG 0.471249 2.4:-25136689 None:intergenic
CCCTAGTCCCATTCTACAGT+TGG 0.473896 2.4:+25136619 MS.gene062845:CDS
GTCTCTTCCTAACCAGCATA+AGG 0.491160 2.4:+25136743 MS.gene062845:CDS
AGTTCATTGCTTGGGCTAAG+AGG 0.495968 2.4:+25136694 MS.gene062845:CDS
AGAATAGCCCTTGCTGAAAA+GGG 0.508196 2.4:+25135558 MS.gene062845:CDS
GGGTTCATTTGTAGAAGATA+AGG 0.510488 2.4:-25135624 None:intergenic
AGAACAAGAAGCTGCCAAGA+AGG 0.534444 2.4:+25136509 MS.gene062845:CDS
CCAATTCATGGCTGATGAGG+TGG 0.538382 2.4:+25135494 None:intergenic
TGTCAATAGATATATGAAGC+TGG 0.543339 2.4:+25136456 MS.gene062845:intron
GTATATTGATGAGGTTTGGA+AGG 0.548215 2.4:+25135716 MS.gene062845:CDS
TGCCATTGTGGATTAGAACC+GGG 0.551246 2.4:-25135662 None:intergenic
CTAGCTTGTGCTCTCAGGTA+GGG 0.551544 2.4:-25135765 None:intergenic
TCTAGCTTGTGCTCTCAGGT+AGG 0.573698 2.4:-25135766 None:intergenic
CTACCAATTCATGGCTGATG+AGG 0.581215 2.4:+25135491 None:intergenic
CCCGGTTCACAAGAAAATCC+CGG 0.589293 2.4:+25135644 MS.gene062845:CDS
GGAGAAACAGCTGGGAGACA+AGG 0.589733 2.4:+25136557 MS.gene062845:CDS
AATAGATATATGAAGCTGGA+AGG 0.589985 2.4:+25136460 MS.gene062845:intron
CCAACTGTAGAATGGGACTA+GGG 0.601552 2.4:-25136619 None:intergenic
GTTCATAGATAAAGATGCAA+GGG 0.604079 2.4:+25135593 MS.gene062845:CDS
ATGCTGGTTAGGAAGAGACT+TGG 0.605348 2.4:-25136739 None:intergenic
GCTCTCAGGTAGGGATCAGA+AGG 0.607625 2.4:-25135756 None:intergenic
ACCAACTGTAGAATGGGACT+AGG 0.615269 2.4:-25136620 None:intergenic
AGTTCATAGATAAAGATGCA+AGG 0.625022 2.4:+25135592 MS.gene062845:CDS
TGAACTCGTAGACCTTATGC+TGG 0.626524 2.4:-25136755 None:intergenic
AAATTGTTGGAGAAACAGCT+GGG 0.647590 2.4:+25136549 MS.gene062845:CDS
TTATCTTCTACAAATGAACC+CGG 0.663975 2.4:+25135626 MS.gene062845:CDS
TAGGGATCAGAAGGCAACAA+CGG 0.667696 2.4:-25135747 None:intergenic
AATTGTTGAGTATATTGATG+AGG 0.670411 2.4:+25135707 MS.gene062845:CDS
ATCCCGGTTCTAATCCACAA+TGG 0.688019 2.4:+25135660 MS.gene062845:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAACAAATAATAAATAT+TGG - chr2.4:25136062-25136081 None:intergenic 10.0%
!!! TTCTTTAATTTTTTTTAAAG+AGG + chr2.4:25136077-25136096 MS.gene062845:intron 10.0%
!! AACAAATAATAAATATTGGA+TGG - chr2.4:25136058-25136077 None:intergenic 15.0%
!! GGATTAAAAATTTATTGAAT+GGG + chr2.4:25136228-25136247 MS.gene062845:intron 15.0%
!! TGGATTAAAAATTTATTGAA+TGG + chr2.4:25136227-25136246 MS.gene062845:intron 15.0%
!!! TTAATTTTTTTTAAAGAGGA+AGG + chr2.4:25136081-25136100 MS.gene062845:intron 15.0%
!! AATGTAATTTGATATTGAGT+AGG - chr2.4:25136265-25136284 None:intergenic 20.0%
!! TACTCAATATCAAATTACAT+TGG + chr2.4:25136264-25136283 MS.gene062845:intron 20.0%
! AAAGTTTATAGGACCAAAAA+AGG + chr2.4:25136483-25136502 MS.gene062845:CDS 25.0%
! AATTAACACCCAAAATTAGT+TGG - chr2.4:25136431-25136450 None:intergenic 25.0%
! AATTGTTGAGTATATTGATG+AGG + chr2.4:25135707-25135726 MS.gene062845:CDS 25.0%
! ACTTAATGCCTAATTTCTTT+AGG - chr2.4:25136791-25136810 None:intergenic 25.0%
! CTTAATGCCTAATTTCTTTA+GGG - chr2.4:25136790-25136809 None:intergenic 25.0%
! GATATTTAATCAGTTGAGTT+AGG + chr2.4:25135956-25135975 MS.gene062845:intron 25.0%
! TGTAGGCAAAAATAAGTTAT+TGG - chr2.4:25136187-25136206 None:intergenic 25.0%
! TTTATTGAATGGGTCTAATA+TGG + chr2.4:25136238-25136257 MS.gene062845:intron 25.0%
!! ACGAATTTTGTAAAATGTGT+AGG - chr2.4:25136204-25136223 None:intergenic 25.0%
!! TTTGAAACATATGAGAAGTT+TGG + chr2.4:25136642-25136661 MS.gene062845:CDS 25.0%
!!! TGTTGTTTCCAACTAATTTT+GGG + chr2.4:25136420-25136439 MS.gene062845:intron 25.0%
AATAGATATATGAAGCTGGA+AGG + chr2.4:25136460-25136479 MS.gene062845:intron 30.0%
AGTTCATAGATAAAGATGCA+AGG + chr2.4:25135592-25135611 MS.gene062845:CDS 30.0%
ATAAAGATGCAAGGGATAAT+TGG + chr2.4:25135601-25135620 MS.gene062845:CDS 30.0%
ATTACAAGTGACTCACAAAT+AGG - chr2.4:25135690-25135709 None:intergenic 30.0%
CATAGATATGCTCAAATTGT+TGG + chr2.4:25136536-25136555 MS.gene062845:CDS 30.0%
CATATCTATGAATTCCTTCT+TGG - chr2.4:25136526-25136545 None:intergenic 30.0%
CCATTTCACTAAAAGTAGTA+AGG + chr2.4:25136357-25136376 MS.gene062845:intron 30.0%
GAGAAGACATCAAATTACAT+TGG + chr2.4:25136138-25136157 MS.gene062845:intron 30.0%
GTTCATAGATAAAGATGCAA+GGG + chr2.4:25135593-25135612 MS.gene062845:CDS 30.0%
TGTCAATAGATATATGAAGC+TGG + chr2.4:25136456-25136475 MS.gene062845:intron 30.0%
TTATCTTCTACAAATGAACC+CGG + chr2.4:25135626-25135645 MS.gene062845:CDS 30.0%
TTGAGTATATTGATGAGGTT+TGG + chr2.4:25135712-25135731 MS.gene062845:CDS 30.0%
TTTCAAACCAACTGTAGAAT+GGG - chr2.4:25136629-25136648 None:intergenic 30.0%
! CATTTTACAAAATTCGTCGT+TGG + chr2.4:25136207-25136226 MS.gene062845:intron 30.0%
! CTTTCATCGATCTCTATTTT+AGG + chr2.4:25136020-25136039 MS.gene062845:intron 30.0%
!! CCTTACTACTTTTAGTGAAA+TGG - chr2.4:25136360-25136379 None:intergenic 30.0%
!!! GTGTTGTTTCCAACTAATTT+TGG + chr2.4:25136419-25136438 MS.gene062845:intron 30.0%
!!! TCGATCTCTATTTTAGGTTT+TGG + chr2.4:25136026-25136045 MS.gene062845:intron 30.0%
!!! TTCTTGTTCTTCTCCTTTTT+TGG - chr2.4:25136499-25136518 None:intergenic 30.0%
AAATTGTTGGAGAAACAGCT+GGG + chr2.4:25136549-25136568 MS.gene062845:CDS 35.0%
AAGCTGGAAGGAAAGTTTAT+AGG + chr2.4:25136472-25136491 MS.gene062845:CDS 35.0%
ATTCTTGCTCTCATTCCATA+AGG - chr2.4:25135543-25135562 None:intergenic 35.0%
GGGTTCATTTGTAGAAGATA+AGG - chr2.4:25135627-25135646 None:intergenic 35.0%
GTATATTGATGAGGTTTGGA+AGG + chr2.4:25135716-25135735 MS.gene062845:CDS 35.0%
GTTTCAAACCAACTGTAGAA+TGG - chr2.4:25136630-25136649 None:intergenic 35.0%
TTGAAACTCACATACACAAG+TGG + chr2.4:25135896-25135915 MS.gene062845:intron 35.0%
! GGCTGACTATGTTGATAAAA+CGG + chr2.4:25135794-25135813 MS.gene062845:CDS 35.0%
!!! CTCTATTTTAGGTTTTGGTG+TGG + chr2.4:25136031-25136050 MS.gene062845:intron 35.0%
AACTCACATACACAAGTGGA+AGG + chr2.4:25135900-25135919 MS.gene062845:intron 40.0%
AAGAATAGCCCTTGCTGAAA+AGG + chr2.4:25135557-25135576 MS.gene062845:CDS 40.0%
AGAATAGCCCTTGCTGAAAA+GGG + chr2.4:25135558-25135577 MS.gene062845:CDS 40.0%
CAAATTGTTGGAGAAACAGC+TGG + chr2.4:25136548-25136567 MS.gene062845:CDS 40.0%
CACAAATAGGTTTGCCATTG+TGG - chr2.4:25135677-25135696 None:intergenic 40.0%
GCAGATGCCCTAAAGAAATT+AGG + chr2.4:25136780-25136799 MS.gene062845:CDS 40.0%
GGCACTCAATCTCTATATTG+AGG - chr2.4:25136671-25136690 None:intergenic 40.0%
TAGCCCAAGCAATGAACTTA+GGG - chr2.4:25136692-25136711 None:intergenic 40.0%
TTAGCCCAAGCAATGAACTT+AGG - chr2.4:25136693-25136712 None:intergenic 40.0%
! AAAATCTAGCTTGTGCTCTC+AGG - chr2.4:25135773-25135792 None:intergenic 40.0%
! GGTTTCTGGATAAGTCCTTA+TGG + chr2.4:25135525-25135544 MS.gene062845:CDS 40.0%
!! GAGAGCACAAGCTAGATTTT+GGG + chr2.4:25135773-25135792 MS.gene062845:CDS 40.0%
!! TATTTTGGAGGAGACAGACT+TGG + chr2.4:25136582-25136601 MS.gene062845:CDS 40.0%
!! TGAGAGCACAAGCTAGATTT+TGG + chr2.4:25135772-25135791 MS.gene062845:CDS 40.0%
!! TTGCCATTGTGGATTAGAAC+CGG - chr2.4:25135666-25135685 None:intergenic 40.0%
!!! AGGTGGTTTTGTTAGGTTTC+TGG + chr2.4:25135511-25135530 MS.gene062845:CDS 40.0%
ACATACACAAGTGGAAGGAC+GGG + chr2.4:25135905-25135924 MS.gene062845:intron 45.0%
ACCAACTGTAGAATGGGACT+AGG - chr2.4:25136623-25136642 None:intergenic 45.0%
AGAACAAGAAGCTGCCAAGA+AGG + chr2.4:25136509-25136528 MS.gene062845:CDS 45.0%
AGTGCCCTAAGTTCATTGCT+TGG + chr2.4:25136685-25136704 MS.gene062845:CDS 45.0%
AGTTCATTGCTTGGGCTAAG+AGG + chr2.4:25136694-25136713 MS.gene062845:CDS 45.0%
CACATACACAAGTGGAAGGA+CGG + chr2.4:25135904-25135923 MS.gene062845:intron 45.0%
CCAACTGTAGAATGGGACTA+GGG - chr2.4:25136622-25136641 None:intergenic 45.0%
GTCTCTTCCTAACCAGCATA+AGG + chr2.4:25136743-25136762 MS.gene062845:CDS 45.0%
GTGCCCTAAGTTCATTGCTT+GGG + chr2.4:25136686-25136705 MS.gene062845:CDS 45.0%
TAGGGATCAGAAGGCAACAA+CGG - chr2.4:25135750-25135769 None:intergenic 45.0%
TGAACTCGTAGACCTTATGC+TGG - chr2.4:25136758-25136777 None:intergenic 45.0%
! ACCGGGATTTTCTTGTGAAC+CGG - chr2.4:25135648-25135667 None:intergenic 45.0%
! ACTTGACACCCTTTTCAGCA+AGG - chr2.4:25135569-25135588 None:intergenic 45.0%
! ATCCCGGTTCTAATCCACAA+TGG + chr2.4:25135660-25135679 MS.gene062845:CDS 45.0%
! ATGCTGGTTAGGAAGAGACT+TGG - chr2.4:25136742-25136761 None:intergenic 45.0%
! CTGGGAGACAAGGCTTATTT+TGG + chr2.4:25136567-25136586 MS.gene062845:CDS 45.0%
! CTTGACACCCTTTTCAGCAA+GGG - chr2.4:25135568-25135587 None:intergenic 45.0%
! TCGTAGACCTTATGCTGGTT+AGG - chr2.4:25136753-25136772 None:intergenic 45.0%
!! TGCCATTGTGGATTAGAACC+GGG - chr2.4:25135665-25135684 None:intergenic 45.0%
!!! GCTGATGAGGTGGTTTTGTT+AGG + chr2.4:25135504-25135523 MS.gene062845:CDS 45.0%
!!! GGAGACAAGGCTTATTTTGG+AGG + chr2.4:25136570-25136589 MS.gene062845:CDS 45.0%
CCCGGTTCACAAGAAAATCC+CGG + chr2.4:25135644-25135663 MS.gene062845:CDS 50.0%
CCCTAGTCCCATTCTACAGT+TGG + chr2.4:25136619-25136638 MS.gene062845:CDS 50.0%
! CCGGGATTTTCTTGTGAACC+GGG - chr2.4:25135647-25135666 None:intergenic 50.0%
! CTAGCTTGTGCTCTCAGGTA+GGG - chr2.4:25135768-25135787 None:intergenic 50.0%
! TCTAGCTTGTGCTCTCAGGT+AGG - chr2.4:25135769-25135788 None:intergenic 50.0%
! TGGAGGAGACAGACTTGGTT+TGG + chr2.4:25136587-25136606 MS.gene062845:CDS 50.0%
GCTCTCAGGTAGGGATCAGA+AGG - chr2.4:25135759-25135778 None:intergenic 55.0%
GGAGAAACAGCTGGGAGACA+AGG + chr2.4:25136557-25136576 MS.gene062845:CDS 55.0%
TACACAAGTGGAAGGACGGG+TGG + chr2.4:25135908-25135927 MS.gene062845:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 25135501 25136812 25135501 ID=MS.gene062845
chr2.4 mRNA 25135501 25136812 25135501 ID=MS.gene062845.t1;Parent=MS.gene062845
chr2.4 exon 25135501 25135815 25135501 ID=MS.gene062845.t1.exon1;Parent=MS.gene062845.t1
chr2.4 CDS 25135501 25135815 25135501 ID=cds.MS.gene062845.t1;Parent=MS.gene062845.t1
chr2.4 exon 25136465 25136812 25136465 ID=MS.gene062845.t1.exon2;Parent=MS.gene062845.t1
chr2.4 CDS 25136465 25136812 25136465 ID=cds.MS.gene062845.t1;Parent=MS.gene062845.t1
Gene Sequence

>MS.gene062845

ATGGCTGATGAGGTGGTTTTGTTAGGTTTCTGGATAAGTCCTTATGGAATGAGAGCAAGAATAGCCCTTGCTGAAAAGGGTGTCAAGTATGAGTTCATAGATAAAGATGCAAGGGATAATTGGCCTTATCTTCTACAAATGAACCCGGTTCACAAGAAAATCCCGGTTCTAATCCACAATGGCAAACCTATTTGTGAGTCACTTGTAATTGTTGAGTATATTGATGAGGTTTGGAAGGATAGATCTCCGTTGTTGCCTTCTGATCCCTACCTGAGAGCACAAGCTAGATTTTGGGCTGACTATGTTGATAAAACGGTATATAATCATAAGAGTCTTCAATTTTTTTTTTTGACTCGTATATTTTTTTTGTACATAGACGAAATGACATATAACTATTGAAACTCACATACACAAGTGGAAGGACGGGTGGTCATAGCATCTGACTTATCAATTTTGATATTTAATCAGTTGAGTTAGGACATGTGCACTGACACGTATATTCAATTTTGCTAAATTATGCTTTCATCGATCTCTATTTTAGGTTTTGGTGTGGTCCATCCAATATTTATTATTTGTTTCTTTAATTTTTTTTAAAGAGGAAGGAAATTTATTACTATTTTTAATTAAAATAGTTTATGAGAAGACATCAAATTACATTGGTATAAACTTAATAGTTACACTGTCCAATAACTTATTTTTGCCTACACATTTTACAAAATTCGTCGTTGGATTAAAAATTTATTGAATGGGTCTAATATGGACCTACTCAATATCAAATTACATTGGTGTGAACATTTTTGTAAGTTCTGATGCAAAAGTTTGTATATCATCAGTATTTTTTGTATTATCAAATCTACCATTTCACTAAAAGTAGTAAGGTCGATCTTTGACTCTTGTGTACGTTTCTCATTTTCTTGCGTGTTGTTTCCAACTAATTTTGGGTGTTAATTATCAATGTCAATAGATATATGAAGCTGGAAGGAAAGTTTATAGGACCAAAAAAGGAGAAGAACAAGAAGCTGCCAAGAAGGAATTCATAGATATGCTCAAATTGTTGGAGAAACAGCTGGGAGACAAGGCTTATTTTGGAGGAGACAGACTTGGTTTGGTAGATATAGCCCTAGTCCCATTCTACAGTTGGTTTGAAACATATGAGAAGTTTGGAAACCTCAATATAGAGATTGAGTGCCCTAAGTTCATTGCTTGGGCTAAGAGGTGCATGCAGATTGAGAGTGTTTCCAAGTCTCTTCCTAACCAGCATAAGGTCTACGAGTTCATTGCAGATGCCCTAAAGAAATTAGGCATTAAGTAG
Protein sequence

>MS.gene062845.t1

MADEVVLLGFWISPYGMRARIALAEKGVKYEFIDKDARDNWPYLLQMNPVHKKIPVLIHNGKPICESLVIVEYIDEVWKDRSPLLPSDPYLRAQARFWADYVDKTIYEAGRKVYRTKKGEEQEAAKKEFIDMLKLLEKQLGDKAYFGGDRLGLVDIALVPFYSWFETYEKFGNLNIEIECPKFIAWAKRCMQIESVSKSLPNQHKVYEFIADALKKLGIK