AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062848

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062848.t1 MTR_2g070150 98.529 204 3 0 1 204 1 204 1.98e-150 416
MS.gene062848.t1 MTR_2g070140 97.044 203 6 0 17 219 1 203 1.49e-147 409
MS.gene062848.t1 MTR_2g070180 88.318 214 25 0 1 214 1 214 3.64e-143 398
MS.gene062848.t1 MTR_2g070130 82.648 219 38 0 1 219 1 219 6.35e-139 388
MS.gene062848.t1 MTR_2g070210 77.376 221 48 1 1 219 1 221 1.17e-129 364
MS.gene062848.t1 MTR_2g070200 73.756 221 56 2 1 219 1 221 2.47e-122 346
MS.gene062848.t1 MTR_2g070060 66.210 219 74 0 1 219 1 219 1.15e-112 321
MS.gene062848.t1 MTR_2g070070 67.123 219 72 0 1 219 1 219 5.19e-110 315
MS.gene062848.t1 MTR_3g467420 66.047 215 73 0 5 219 10 224 1.25e-108 311
MS.gene062848.t1 MTR_8g056940 66.210 219 74 0 1 219 1 219 5.53e-105 302
MS.gene062848.t1 MTR_3g467430 63.134 217 80 0 3 219 8 224 1.57e-103 298
MS.gene062848.t1 MTR_2g070110 63.470 219 79 1 1 219 1 218 8.20e-102 294
MS.gene062848.t1 MTR_4g124130 61.364 220 84 1 1 219 1 220 7.33e-98 284
MS.gene062848.t1 MTR_2g070120 61.502 213 82 0 1 213 1 213 2.42e-95 277
MS.gene062848.t1 MTR_5g076900 53.881 219 99 2 2 219 4 221 5.57e-83 246
MS.gene062848.t1 MTR_0186s0030 69.504 141 43 0 1 141 1 141 2.33e-67 204
MS.gene062848.t1 MTR_1g090060 44.286 210 112 3 4 209 3 211 2.29e-55 176
MS.gene062848.t1 MTR_8g087425 43.318 217 120 2 2 216 3 218 9.53e-55 174
MS.gene062848.t1 MTR_4g059730 43.636 220 117 4 1 215 1 218 2.14e-52 168
MS.gene062848.t1 MTR_7g065740 45.024 211 108 5 3 208 6 213 1.13e-51 167
MS.gene062848.t1 MTR_7g065720 43.128 211 112 5 3 208 6 213 5.97e-51 165
MS.gene062848.t1 MTR_4g019790 42.788 208 114 4 4 208 7 212 1.48e-50 164
MS.gene062848.t1 MTR_3g099757 42.927 205 114 2 3 205 4 207 2.89e-50 163
MS.gene062848.t1 MTR_7g065750 43.541 209 112 4 3 207 6 212 5.55e-50 162
MS.gene062848.t1 MTR_5g037380 43.662 213 111 5 2 205 3 215 7.63e-50 162
MS.gene062848.t1 MTR_4g019780 40.865 208 120 2 3 208 7 213 8.45e-50 162
MS.gene062848.t1 MTR_8g087410 43.627 204 112 2 4 205 6 208 1.15e-49 161
MS.gene062848.t1 MTR_7g065660 41.429 210 118 4 3 209 6 213 1.76e-49 161
MS.gene062848.t1 MTR_5g040430 37.915 211 124 2 2 205 5 215 2.86e-49 160
MS.gene062848.t1 MTR_1g090100 44.712 208 110 3 5 208 3 209 5.32e-49 160
MS.gene062848.t1 MTR_7g065630 41.148 209 114 5 3 208 24 226 8.94e-48 157
MS.gene062848.t1 MTR_1g090070 40.376 213 117 5 4 209 9 218 7.68e-47 154
MS.gene062848.t1 MTR_1g090150 38.739 222 122 6 3 216 2 217 1.87e-46 153
MS.gene062848.t1 MTR_6g080440 42.408 191 105 3 18 205 17 205 4.20e-46 152
MS.gene062848.t1 MTR_7g065710 40.670 209 119 5 1 208 1 205 7.30e-46 152
MS.gene062848.t1 MTR_7g065270 41.148 209 118 5 3 208 6 212 3.52e-45 150
MS.gene062848.t1 MTR_7g065265 41.627 209 117 5 3 208 6 212 1.02e-44 149
MS.gene062848.t1 MTR_7g065290 41.627 209 117 5 3 208 6 212 1.02e-44 149
MS.gene062848.t1 MTR_7g065590 38.095 210 125 4 3 209 6 213 2.03e-44 148
MS.gene062848.t1 MTR_1g090090 42.105 209 116 4 4 208 3 210 6.85e-44 147
MS.gene062848.t1 MTR_7g065600 38.756 209 123 4 3 208 6 212 1.01e-43 146
MS.gene062848.t1 MTR_7g065700 42.584 209 115 5 1 208 4 208 1.21e-43 146
MS.gene062848.t1 MTR_7g065230 41.346 208 114 5 3 208 6 207 1.45e-43 145
MS.gene062848.t1 MTR_8g061950 38.208 212 122 3 3 205 4 215 3.23e-43 145
MS.gene062848.t1 MTR_1g115195 40.952 210 117 5 1 205 1 208 6.73e-43 144
MS.gene062848.t1 MTR_7g065680 41.148 209 114 5 3 208 6 208 6.81e-43 144
MS.gene062848.t1 MTR_7g065640 38.164 207 109 4 3 208 6 194 2.19e-41 140
MS.gene062848.t1 MTR_7g065260 39.303 201 117 5 3 200 13 211 3.38e-36 127
MS.gene062848.t1 MTR_2g072120 64.384 73 25 1 47 118 1 73 1.11e-30 108
MS.gene062848.t1 MTR_4g134370 30.114 176 96 5 3 159 14 181 3.53e-14 69.3
MS.gene062848.t1 MTR_4g134380 31.579 171 98 5 4 159 15 181 4.04e-14 69.3
MS.gene062848.t1 MTR_4g134380 31.515 165 94 5 4 153 15 175 6.26e-13 65.1
MS.gene062848.t1 MTR_4g043640 57.143 42 18 0 148 189 44 85 9.16e-12 59.7
MS.gene062848.t1 MTR_1g067180 27.363 201 131 7 11 205 31 222 7.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062848.t1 AT1G78380 70.370 216 64 0 1 216 1 216 5.68e-115 327
MS.gene062848.t1 AT1G17180 68.037 219 70 0 1 219 1 219 6.58e-115 327
MS.gene062848.t1 AT1G78340 67.290 214 70 0 1 214 1 214 4.22e-110 315
MS.gene062848.t1 AT1G17170 66.667 219 72 1 1 219 1 218 7.88e-110 314
MS.gene062848.t1 AT1G78370 66.822 214 71 0 1 214 1 214 8.20e-109 311
MS.gene062848.t1 AT1G78360 65.455 220 75 1 1 219 1 220 2.75e-108 310
MS.gene062848.t1 AT1G78320 65.421 214 74 0 1 214 1 214 1.21e-105 303
MS.gene062848.t1 AT1G17190 58.605 215 89 0 3 217 4 218 2.53e-97 283
MS.gene062848.t1 AT1G53680 58.447 219 89 2 3 219 6 224 3.22e-96 280
MS.gene062848.t1 AT3G43800 57.407 216 88 3 3 214 4 219 2.32e-85 253
MS.gene062848.t1 AT1G78320 56.075 214 67 1 1 214 1 187 6.63e-83 245
MS.gene062848.t1 AT2G29420 42.381 210 118 2 2 209 7 215 2.75e-57 181
MS.gene062848.t1 AT3G09270 42.180 211 117 4 3 209 5 214 2.00e-53 171
MS.gene062848.t1 AT2G29450 45.411 207 106 5 3 204 5 209 2.67e-52 168
MS.gene062848.t1 AT2G29440 44.660 206 108 4 3 204 5 208 5.37e-52 167
MS.gene062848.t1 AT2G29480 42.718 206 112 4 3 204 6 209 1.31e-51 166
MS.gene062848.t1 AT2G29490 42.233 206 113 4 3 204 6 209 1.60e-51 166
MS.gene062848.t1 AT1G10360 42.791 215 115 4 2 208 3 217 2.77e-50 163
MS.gene062848.t1 AT1G59700 42.922 219 116 5 3 212 5 223 3.03e-49 160
MS.gene062848.t1 AT2G29460 40.291 206 117 4 3 204 6 209 4.81e-48 157
MS.gene062848.t1 AT1G74590 36.323 223 128 3 3 215 6 224 1.29e-47 156
MS.gene062848.t1 AT2G29470 40.488 205 115 5 5 204 8 210 2.18e-47 155
MS.gene062848.t1 AT1G59670 42.152 223 118 5 3 215 5 226 1.07e-46 154
MS.gene062848.t1 AT1G10370 39.640 222 126 4 2 215 3 224 1.77e-46 154
MS.gene062848.t1 AT1G27130 37.168 226 130 5 3 219 5 227 2.10e-44 148
MS.gene062848.t1 AT1G27140 39.631 217 121 4 3 209 5 221 1.06e-42 144
MS.gene062848.t1 AT1G69930 38.389 211 123 3 5 208 13 223 1.59e-42 144
MS.gene062848.t1 AT5G62480 36.000 225 128 4 3 216 7 226 2.64e-42 143
MS.gene062848.t1 AT1G69920 37.168 226 124 6 5 218 35 254 1.50e-40 139
MS.gene062848.t1 AT5G62480 33.143 175 81 3 3 168 7 154 2.65e-25 98.6
MS.gene062848.t1 AT5G62480 33.143 175 80 3 3 168 7 153 2.85e-25 98.6

Find 59 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCTACACTTGGTTTAA+AGG 0.105597 2.4:+25183947 MS.gene062848:CDS
GCTCTTGCTCCAAGAGTTTA+AGG 0.122619 2.4:-25183865 None:intergenic
AGAGATCACAAGCTAGATTT+TGG 0.158738 2.4:+25182707 MS.gene062848:CDS
AGTACAGAGAAGAAGATTTA+AGG 0.220231 2.4:+25182527 MS.gene062848:CDS
ATCCCTGTTCTTATTCATAA+TGG 0.299948 2.4:+25182595 MS.gene062848:CDS
GGCTTCTATGAATTCCTTCT+TGG 0.303918 2.4:-25183843 None:intergenic
TTCAGTATATTGATGAGGTT+TGG 0.327749 2.4:+25182647 MS.gene062848:CDS
AAGTGATTCTGCTAGATTAC+TGG 0.343179 2.4:+25182446 MS.gene062848:CDS
CATAGAAGCCCTTAAACTCT+TGG 0.363135 2.4:+25183856 MS.gene062848:CDS
CCTGATCAAGATAAGGTCTA+TGG 0.369349 2.4:+25184070 MS.gene062848:CDS
CTCAACCTGCATGCATCTCT+TGG 0.377767 2.4:-25184029 None:intergenic
CCATAGACCTTATCTTGATC+AGG 0.389501 2.4:-25184070 None:intergenic
CACTTATTCCATTCTACACT+TGG 0.395366 2.4:+25183939 MS.gene062848:CDS
TTACCATTATGAATAAGAAC+AGG 0.399703 2.4:-25182598 None:intergenic
AAGAATAGCCCTAGCTGAAA+AGG 0.402786 2.4:+25182492 MS.gene062848:CDS
GTGGAGATCAGGAAGAAGTT+TGG 0.409480 2.4:+25184100 MS.gene062848:CDS
CTCTTGCTCCAAGAGTTTAA+GGG 0.414095 2.4:-25183864 None:intergenic
ATGAAGTTGGAAGGAACCTT+TGG 0.415645 2.4:+25183792 MS.gene062848:CDS
AGCAATTAAAGATTCACAAA+TGG 0.421376 2.4:-25182623 None:intergenic
GGCTGATTATGTTGATAAGA+AGG 0.427339 2.4:+25182729 MS.gene062848:CDS
GCAATTAAAGATTCACAAAT+GGG 0.429408 2.4:-25182622 None:intergenic
TTTAAAGGCTATGAGACCTT+TGG 0.432290 2.4:+25183962 MS.gene062848:CDS
GATTACTGGGTAAGTCCATT+TGG 0.433074 2.4:+25182460 MS.gene062848:CDS
TAAACTCTTGGAGCAAGAGC+TGG 0.443964 2.4:+25183868 MS.gene062848:CDS
CTGGGAGACAAGACTTACTT+TGG 0.447631 2.4:+25183887 MS.gene062848:CDS
CTTGGCCCAAGCAATGAACT+TGG 0.448713 2.4:-25184011 None:intergenic
TACTTTGGAGGAGACAAGAT+TGG 0.453564 2.4:+25183902 MS.gene062848:CDS
ATGCCCCAAGTTCATTGCTT+GGG 0.459033 2.4:+25184006 MS.gene062848:CDS
ATGGCTTCATTGTGGAGATC+AGG 0.465214 2.4:+25184089 MS.gene062848:CDS
CTAGCTTGTGATCTCTGATA+AGG 0.467136 2.4:-25182700 None:intergenic
AATGCCCCAAGTTCATTGCT+TGG 0.475316 2.4:+25184005 MS.gene062848:CDS
GTGAAATAGATCTATGAAGT+TGG 0.485653 2.4:+25183779 MS.gene062848:intron
GGTTCATTTGTAACAACAAA+GGG 0.491203 2.4:-25182558 None:intergenic
ATTCTTACCCTCATGCCAAA+TGG 0.499058 2.4:-25182475 None:intergenic
CCTTTAAACCAAGTGTAGAA+TGG 0.500913 2.4:-25183947 None:intergenic
TTGGCCCAAGCAATGAACTT+GGG 0.508586 2.4:-25184010 None:intergenic
GGAGACAAGACTTACTTTGG+AGG 0.510834 2.4:+25183890 MS.gene062848:CDS
AGAACAAGAAGCTGCCAAGA+AGG 0.534444 2.4:+25183829 MS.gene062848:CDS
CTCTACATTGATATTGCCAA+AGG 0.536889 2.4:-25183978 None:intergenic
AGAATAGCCCTAGCTGAAAA+GGG 0.541187 2.4:+25182493 MS.gene062848:CDS
GTCACTCCCTGATCAAGATA+AGG 0.545449 2.4:+25184063 MS.gene062848:CDS
AATAGATCTATGAAGTTGGA+AGG 0.560610 2.4:+25183783 MS.gene062848:intron
AGTGATTCTGCTAGATTACT+GGG 0.564225 2.4:+25182447 MS.gene062848:CDS
GGTAAGTCCATTTGGCATGA+GGG 0.564671 2.4:+25182468 MS.gene062848:CDS
AAACTCTTGGAGCAAGAGCT+GGG 0.566713 2.4:+25183869 MS.gene062848:CDS
CATAGACCTTATCTTGATCA+GGG 0.570459 2.4:-25184069 None:intergenic
TGCTGTTCAGTATATTGATG+AGG 0.571065 2.4:+25182642 MS.gene062848:CDS
GAAGAAGTTTGGCCTTGAGT+AGG 0.573969 2.4:+25184111 MS.gene062848:CDS
TGGCAATATCAATGTAGAGA+AGG 0.574601 2.4:+25183982 MS.gene062848:CDS
TGGCCCAAGCAATGAACTTG+GGG 0.582333 2.4:-25184009 None:intergenic
TTGGGCCAAGAGATGCATGC+AGG 0.591022 2.4:+25184024 MS.gene062848:CDS
GATCTCTGATAAGGATCAGA+AGG 0.596783 2.4:-25182691 None:intergenic
GGGTTCATTTGTAACAACAA+AGG 0.598423 2.4:-25182559 None:intergenic
GGGTAAGTCCATTTGGCATG+AGG 0.600109 2.4:+25182467 MS.gene062848:CDS
ATCTTGATCAGGGAGTGACT+TGG 0.607214 2.4:-25184059 None:intergenic
TAAGGATCAGAAGGCAACAA+AGG 0.613337 2.4:-25182682 None:intergenic
TGACATCTCCAACCTACTCA+AGG 0.625823 2.4:-25184123 None:intergenic
TAAGGTCTATGGCTTCATTG+TGG 0.637923 2.4:+25184081 MS.gene062848:CDS
TACCATTATGAATAAGAACA+GGG 0.645194 2.4:-25182597 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAGTTAATTATACAAATA+AGG + chr2.4:25183557-25183576 MS.gene062848:intron 15.0%
!! AGAGTTAATTATACAAATAA+GGG + chr2.4:25183558-25183577 MS.gene062848:intron 15.0%
!! AATAAAACTTTCATCAACAA+GGG - chr2.4:25182848-25182867 None:intergenic 20.0%
!! TAATTAACTCTTAGAAATAG+TGG - chr2.4:25183549-25183568 None:intergenic 20.0%
!! TATGTATCTTAACTATTTGA+AGG + chr2.4:25183600-25183619 MS.gene062848:intron 20.0%
!!! TATTTTGAATGACTTATTAG+AGG + chr2.4:25182967-25182986 MS.gene062848:intron 20.0%
!!! TTCAAAATAAGCTCTAAAAA+AGG - chr2.4:25182956-25182975 None:intergenic 20.0%
! AGCAATTAAAGATTCACAAA+TGG - chr2.4:25182626-25182645 None:intergenic 25.0%
! ATCATCTTAGAAATTGTGAT+TGG - chr2.4:25183336-25183355 None:intergenic 25.0%
! ATTTGAAGGTAGTTGAAATT+TGG + chr2.4:25183614-25183633 MS.gene062848:intron 25.0%
! GAATAAAACTTTCATCAACA+AGG - chr2.4:25182849-25182868 None:intergenic 25.0%
! GCAATTAAAGATTCACAAAT+GGG - chr2.4:25182625-25182644 None:intergenic 25.0%
! TACCATTATGAATAAGAACA+GGG - chr2.4:25182600-25182619 None:intergenic 25.0%
! TTACCATTATGAATAAGAAC+AGG - chr2.4:25182601-25182620 None:intergenic 25.0%
!! CATATAATTGATTCATGTGA+CGG - chr2.4:25183584-25183603 None:intergenic 25.0%
!! CTCTAAAAAAACGATTTATG+AGG - chr2.4:25183301-25183320 None:intergenic 25.0%
!! GCTTAGAATTTGTTTATCAT+TGG + chr2.4:25182804-25182823 MS.gene062848:intron 25.0%
!! GCTTATTAAAATGTGCTTTT+CGG + chr2.4:25183064-25183083 MS.gene062848:intron 25.0%
!!! CTCATAAATCGTTTTTTTAG+AGG + chr2.4:25183299-25183318 MS.gene062848:intron 25.0%
!!! GTTTTTTTAGAGGTTGAATT+AGG + chr2.4:25183309-25183328 MS.gene062848:intron 25.0%
AATAAGCTAAAAGCAGATCA+TGG - chr2.4:25183051-25183070 None:intergenic 30.0%
AATAGATCTATGAAGTTGGA+AGG + chr2.4:25183783-25183802 MS.gene062848:intron 30.0%
AGTACAGAGAAGAAGATTTA+AGG + chr2.4:25182527-25182546 MS.gene062848:CDS 30.0%
ATCCCTGTTCTTATTCATAA+TGG + chr2.4:25182595-25182614 MS.gene062848:CDS 30.0%
ATGGTTCATGTGTTTATAAG+TGG + chr2.4:25183473-25183492 MS.gene062848:intron 30.0%
ATTGTTAGAGAGTCTCAAAT+CGG + chr2.4:25183244-25183263 MS.gene062848:intron 30.0%
GAACATGTATTTATAAGTGG+AGG + chr2.4:25183266-25183285 MS.gene062848:intron 30.0%
GGTTCATTTGTAACAACAAA+GGG - chr2.4:25182561-25182580 None:intergenic 30.0%
GTGAAATAGATCTATGAAGT+TGG + chr2.4:25183779-25183798 MS.gene062848:intron 30.0%
TAACTCTTAGAAATAGTGGT+CGG - chr2.4:25183545-25183564 None:intergenic 30.0%
TCGGAACATGTATTTATAAG+TGG + chr2.4:25183263-25183282 MS.gene062848:intron 30.0%
TGGTTCATGTGTTTATAAGT+GGG + chr2.4:25183474-25183493 MS.gene062848:intron 30.0%
TTCAGTATATTGATGAGGTT+TGG + chr2.4:25182647-25182666 MS.gene062848:CDS 30.0%
!! TTGATATTTTTCAGCATGCA+CGG + chr2.4:25183667-25183686 MS.gene062848:intron 30.0%
!!! TTCTTGTTCTTCTCCTTTTT+TGG - chr2.4:25183819-25183838 None:intergenic 30.0%
AACTCTTAGAAATAGTGGTC+GGG - chr2.4:25183544-25183563 None:intergenic 35.0%
AAGTGATTCTGCTAGATTAC+TGG + chr2.4:25182446-25182465 MS.gene062848:CDS 35.0%
AGTGATTCTGCTAGATTACT+GGG + chr2.4:25182447-25182466 MS.gene062848:CDS 35.0%
ATAATTCTCTCCACACAAGT+CGG + chr2.4:25183498-25183517 MS.gene062848:intron 35.0%
ATGCAATGAATCGTACAAAC+GGG - chr2.4:25182771-25182790 None:intergenic 35.0%
CACTTATTCCATTCTACACT+TGG + chr2.4:25183939-25183958 MS.gene062848:CDS 35.0%
CATAGACCTTATCTTGATCA+GGG - chr2.4:25184072-25184091 None:intergenic 35.0%
CATGCAATGAATCGTACAAA+CGG - chr2.4:25182772-25182791 None:intergenic 35.0%
CCATTCTACACTTGGTTTAA+AGG + chr2.4:25183947-25183966 MS.gene062848:CDS 35.0%
CCTTTAAACCAAGTGTAGAA+TGG - chr2.4:25183950-25183969 None:intergenic 35.0%
CTCTACATTGATATTGCCAA+AGG - chr2.4:25183981-25184000 None:intergenic 35.0%
GACAAAAGATGACATGAACA+TGG + chr2.4:25183454-25183473 MS.gene062848:intron 35.0%
GCTATCATGTTTCCGTTATT+GGG + chr2.4:25183390-25183409 MS.gene062848:intron 35.0%
GGGTTCATTTGTAACAACAA+AGG - chr2.4:25182562-25182581 None:intergenic 35.0%
GGTTCATGTGTTTATAAGTG+GGG + chr2.4:25183475-25183494 MS.gene062848:intron 35.0%
TGCTATCATGTTTCCGTTAT+TGG + chr2.4:25183389-25183408 MS.gene062848:intron 35.0%
TGCTGTTCAGTATATTGATG+AGG + chr2.4:25182642-25182661 MS.gene062848:CDS 35.0%
TGGCAATATCAATGTAGAGA+AGG + chr2.4:25183982-25184001 MS.gene062848:CDS 35.0%
TGGTTCGTAGACTAAATGAT+GGG - chr2.4:25183419-25183438 None:intergenic 35.0%
TTCATCAACAAGGGACAAAA+GGG - chr2.4:25182839-25182858 None:intergenic 35.0%
TTTAAAGGCTATGAGACCTT+TGG + chr2.4:25183962-25183981 MS.gene062848:CDS 35.0%
TTTCATCAACAAGGGACAAA+AGG - chr2.4:25182840-25182859 None:intergenic 35.0%
! ACAGGGATTTTCTTATGAAC+AGG - chr2.4:25182583-25182602 None:intergenic 35.0%
! AGAGATCACAAGCTAGATTT+TGG + chr2.4:25182707-25182726 MS.gene062848:CDS 35.0%
! ATCTTTTGTCCGATGTGATT+GGG - chr2.4:25183444-25183463 None:intergenic 35.0%
! CAGGGATTTTCTTATGAACA+GGG - chr2.4:25182582-25182601 None:intergenic 35.0%
! GACATGTGAATAAGCTGTTT+TGG - chr2.4:25183029-25183048 None:intergenic 35.0%
! GAGATCACAAGCTAGATTTT+GGG + chr2.4:25182708-25182727 MS.gene062848:CDS 35.0%
! GGCTGATTATGTTGATAAGA+AGG + chr2.4:25182729-25182748 MS.gene062848:CDS 35.0%
! TTGGTTCGTAGACTAAATGA+TGG - chr2.4:25183420-25183439 None:intergenic 35.0%
! TTTGATACCCTTTTCAGCTA+GGG - chr2.4:25182503-25182522 None:intergenic 35.0%
AAGAATAGCCCTAGCTGAAA+AGG + chr2.4:25182492-25182511 MS.gene062848:CDS 40.0%
AGAATAGCCCTAGCTGAAAA+GGG + chr2.4:25182493-25182512 MS.gene062848:CDS 40.0%
AGGAACCTTTGGACCAAAAA+AGG + chr2.4:25183803-25183822 MS.gene062848:CDS 40.0%
ATGAAGTTGGAAGGAACCTT+TGG + chr2.4:25183792-25183811 MS.gene062848:CDS 40.0%
ATTCTTACCCTCATGCCAAA+TGG - chr2.4:25182478-25182497 None:intergenic 40.0%
CATAGAAGCCCTTAAACTCT+TGG + chr2.4:25183856-25183875 MS.gene062848:CDS 40.0%
CCATAGACCTTATCTTGATC+AGG - chr2.4:25184073-25184092 None:intergenic 40.0%
CCTGATCAAGATAAGGTCTA+TGG + chr2.4:25184070-25184089 MS.gene062848:CDS 40.0%
CTCTTGCTCCAAGAGTTTAA+GGG - chr2.4:25183867-25183886 None:intergenic 40.0%
GAGAAAAACAACAGCTACCT+AGG - chr2.4:25183719-25183738 None:intergenic 40.0%
GATCTCTGATAAGGATCAGA+AGG - chr2.4:25182694-25182713 None:intergenic 40.0%
GATTACTGGGTAAGTCCATT+TGG + chr2.4:25182460-25182479 MS.gene062848:CDS 40.0%
GGCTTCTATGAATTCCTTCT+TGG - chr2.4:25183846-25183865 None:intergenic 40.0%
TAAGGATCAGAAGGCAACAA+AGG - chr2.4:25182685-25182704 None:intergenic 40.0%
TAAGGTCTATGGCTTCATTG+TGG + chr2.4:25184081-25184100 MS.gene062848:CDS 40.0%
! CATCTTTTGTCCGATGTGAT+TGG - chr2.4:25183445-25183464 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG - chr2.4:25182703-25182722 None:intergenic 40.0%
! GTTTGATACCCTTTTCAGCT+AGG - chr2.4:25182504-25182523 None:intergenic 40.0%
! TACTTTGGAGGAGACAAGAT+TGG + chr2.4:25183902-25183921 MS.gene062848:CDS 40.0%
!! AATGATGGGTAGCCCAATAA+CGG - chr2.4:25183405-25183424 None:intergenic 40.0%
!!! AGGAGACAAGATTGGTTTTG+TGG + chr2.4:25183910-25183929 MS.gene062848:CDS 40.0%
!!! CTTCTCCTTTTTTGGTCCAA+AGG - chr2.4:25183811-25183830 None:intergenic 40.0%
AAACAACAGCTACCTAGGCT+AGG - chr2.4:25183714-25183733 None:intergenic 45.0%
AAACTCTTGGAGCAAGAGCT+GGG + chr2.4:25183869-25183888 MS.gene062848:CDS 45.0%
AATGCCCCAAGTTCATTGCT+TGG + chr2.4:25184005-25184024 MS.gene062848:CDS 45.0%
AGAACAAGAAGCTGCCAAGA+AGG + chr2.4:25183829-25183848 MS.gene062848:CDS 45.0%
ATCTTGATCAGGGAGTGACT+TGG - chr2.4:25184062-25184081 None:intergenic 45.0%
ATGCCCCAAGTTCATTGCTT+GGG + chr2.4:25184006-25184025 MS.gene062848:CDS 45.0%
ATGGCTTCATTGTGGAGATC+AGG + chr2.4:25184089-25184108 MS.gene062848:CDS 45.0%
CTGGGAGACAAGACTTACTT+TGG + chr2.4:25183887-25183906 MS.gene062848:CDS 45.0%
GCAAGTAATAGTCCTAGCCT+AGG + chr2.4:25183699-25183718 MS.gene062848:intron 45.0%
GCTCTTGCTCCAAGAGTTTA+AGG - chr2.4:25183868-25183887 None:intergenic 45.0%
GGAGACAAGACTTACTTTGG+AGG + chr2.4:25183890-25183909 MS.gene062848:CDS 45.0%
GGTAAGTCCATTTGGCATGA+GGG + chr2.4:25182468-25182487 MS.gene062848:CDS 45.0%
GTCACTCCCTGATCAAGATA+AGG + chr2.4:25184063-25184082 MS.gene062848:CDS 45.0%
GTGGAGATCAGGAAGAAGTT+TGG + chr2.4:25184100-25184119 MS.gene062848:CDS 45.0%
TAAACTCTTGGAGCAAGAGC+TGG + chr2.4:25183868-25183887 MS.gene062848:CDS 45.0%
TTGGCCCAAGCAATGAACTT+GGG - chr2.4:25184013-25184032 None:intergenic 45.0%
CGAACCAAGCCCAATCACAT+CGG + chr2.4:25183432-25183451 MS.gene062848:intron 50.0%
CTCAACCTGCATGCATCTCT+TGG - chr2.4:25184032-25184051 None:intergenic 50.0%
CTTGGCCCAAGCAATGAACT+TGG - chr2.4:25184014-25184033 None:intergenic 50.0%
GGGTAAGTCCATTTGGCATG+AGG + chr2.4:25182467-25182486 MS.gene062848:CDS 50.0%
TGGCCCAAGCAATGAACTTG+GGG - chr2.4:25184012-25184031 None:intergenic 50.0%
TTGTCCGATGTGATTGGGCT+TGG - chr2.4:25183439-25183458 None:intergenic 50.0%
!! CACCACAAAACCGACTTGTG+TGG - chr2.4:25183511-25183530 None:intergenic 50.0%
!! CTCCACACAAGTCGGTTTTG+TGG + chr2.4:25183506-25183525 MS.gene062848:intron 50.0%
TTGGGCCAAGAGATGCATGC+AGG + chr2.4:25184024-25184043 MS.gene062848:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 25182436 25184132 25182436 ID=MS.gene062848
chr2.4 mRNA 25182436 25184132 25182436 ID=MS.gene062848.t1;Parent=MS.gene062848
chr2.4 exon 25182436 25182750 25182436 ID=MS.gene062848.t1.exon1;Parent=MS.gene062848.t1
chr2.4 CDS 25182436 25182750 25182436 ID=cds.MS.gene062848.t1;Parent=MS.gene062848.t1
chr2.4 exon 25183788 25184132 25183788 ID=MS.gene062848.t1.exon2;Parent=MS.gene062848.t1
chr2.4 CDS 25183788 25184132 25183788 ID=cds.MS.gene062848.t1;Parent=MS.gene062848.t1
Gene Sequence

>MS.gene062848

ATGGCTGATGAAGTGATTCTGCTAGATTACTGGGTAAGTCCATTTGGCATGAGGGTAAGAATAGCCCTAGCTGAAAAGGGTATCAAACATGAGTACAGAGAAGAAGATTTAAGGAACAAGAGCCCTTTGTTGTTACAAATGAACCCTGTTCATAAGAAAATCCCTGTTCTTATTCATAATGGTAAACCCATTTGTGAATCTTTAATTGCTGTTCAGTATATTGATGAGGTTTGGAATGATAAGTCTCCTTTGTTGCCTTCTGATCCTTATCAGAGATCACAAGCTAGATTTTGGGCTGATTATGTTGATAAGAAGGTATATAAATTTACATACCCGTTTGTACGATTCATTGCATGTCATTGTTTGTTGCTTAGAATTTGTTTATCATTGGTTTTGTTGACCCTTTTGTCCCTTGTTGATGAAAGTTTTATTCTTTTGCATTTTTCAGTATTAATTCTTTAATTAATTCTTTTGCATGTGATTGTTTGTGTTTTTTTTTTACATTTAAGAAATTAAACCTTTTTTAGAGCTTATTTTGAATGACTTATTAGAGGTTTATAGCATATTTGAGACTGTTTTCTGAGAGTTTACCAAAACAGCTTATTCACATGTCCATGATCTGCTTTTAGCTTATTAAAATGTGCTTTTCGGCTTAGTTTCATAACTCAAAGATAGCTTGCTTATGAAAGCAACTTATAGTTTATATACACTTATAAATAAGTTTACATAAATTAATTTTTGCTTTAAAAATAGTTTGTATATGAGCACTTCTATGATAAAACTAGCTACTTAACTAAGCTATTAAGTTATTGTTAGAGAGTCTCAAATCGGAACATGTATTTATAAGTGGAGGCAGTTCTCGCCTCATAAATCGTTTTTTTAGAGGTTGAATTAGGTCCAATCACAATTTCTAAGATGATATCAAAGTCTCTGATAAGATCTATTGAGTCACATGCTATCATGTTTCCGTTATTGGGCTACCCATCATTTAGTCTACGAACCAAGCCCAATCACATCGGACAAAAGATGACATGAACATGGTTCATGTGTTTATAAGTGGGGATAATTCTCTCCACACAAGTCGGTTTTGTGGTGTTGTGTTAGACCCGACCACTATTTCTAAGAGTTAATTATACAAATAAGGGCCGTCACATGAATCAATTATATGTATCTTAACTATTTGAAGGTAGTTGAAATTTGGAACACTTTTCTTTTGAACAATACAAGCTATTTGATATTTTTCAGCATGCACGGTCACTATTAGCAAGTAATAGTCCTAGCCTAGGTAGCTGTTGTTTTTCTCGTTATTGCATTTGATGATCTAATTGTGTGTGATTCTCATTGTGAAATAGATCTATGAAGTTGGAAGGAACCTTTGGACCAAAAAAGGAGAAGAACAAGAAGCTGCCAAGAAGGAATTCATAGAAGCCCTTAAACTCTTGGAGCAAGAGCTGGGAGACAAGACTTACTTTGGAGGAGACAAGATTGGTTTTGTGGATGTAGCACTTATTCCATTCTACACTTGGTTTAAAGGCTATGAGACCTTTGGCAATATCAATGTAGAGAAGGAATGCCCCAAGTTCATTGCTTGGGCCAAGAGATGCATGCAGGTTGAGAGTGTTTCCAAGTCACTCCCTGATCAAGATAAGGTCTATGGCTTCATTGTGGAGATCAGGAAGAAGTTTGGCCTTGAGTAG
Protein sequence

>MS.gene062848.t1

MADEVILLDYWVSPFGMRVRIALAEKGIKHEYREEDLRNKSPLLLQMNPVHKKIPVLIHNGKPICESLIAVQYIDEVWNDKSPLLPSDPYQRSQARFWADYVDKKIYEVGRNLWTKKGEEQEAAKKEFIEALKLLEQELGDKTYFGGDKIGFVDVALIPFYTWFKGYETFGNINVEKECPKFIAWAKRCMQVESVSKSLPDQDKVYGFIVEIRKKFGLE