Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92548.t1 | XP_013454941.1 | 98.4 | 255 | 4 | 0 | 1 | 255 | 1 | 255 | 2.80E-144 | 521.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92548.t1 | Q93YV9 | 32.1 | 109 | 70 | 2 | 134 | 242 | 107 | 211 | 3.7e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92548.t1 | A0A072UGV8 | 98.4 | 255 | 4 | 0 | 1 | 255 | 1 | 255 | 2.0e-144 | 521.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene92548 | 0.80249 | 5.47E-49 | -1.69E-46 |
| MS.gene049151 | MS.gene92548 | 0.820296 | 7.39E-53 | -1.69E-46 |
| MS.gene049195 | MS.gene92548 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene049230 | MS.gene92548 | 0.837917 | 3.92E-57 | -1.69E-46 |
| MS.gene049260 | MS.gene92548 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene049297 | MS.gene92548 | 0.872129 | 4.03E-67 | -1.69E-46 |
| MS.gene04933 | MS.gene92548 | 0.816853 | 4.46E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene92548 | 0.808507 | 2.99E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene92548 | 0.893883 | 4.15E-75 | -1.69E-46 |
| MS.gene049599 | MS.gene92548 | -0.830655 | 2.60E-55 | -1.69E-46 |
| MS.gene049629 | MS.gene92548 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene049669 | MS.gene92548 | 0.806455 | 8.16E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene92548 | 0.8872 | 1.76E-72 | -1.69E-46 |
| MS.gene04984 | MS.gene92548 | 0.825652 | 4.17E-54 | -1.69E-46 |
| MS.gene049910 | MS.gene92548 | 0.842786 | 2.09E-58 | -1.69E-46 |
| MS.gene049915 | MS.gene92548 | 0.850025 | 2.22E-60 | -1.69E-46 |
| MS.gene050226 | MS.gene92548 | 0.87551 | 2.91E-68 | -1.69E-46 |
| MS.gene050227 | MS.gene92548 | 0.897562 | 1.25E-76 | -1.69E-46 |
| MS.gene050228 | MS.gene92548 | 0.881279 | 2.74E-70 | -1.69E-46 |
| MS.gene050301 | MS.gene92548 | 0.835894 | 1.29E-56 | -1.69E-46 |
| MS.gene050303 | MS.gene92548 | 0.839336 | 1.68E-57 | -1.69E-46 |
| MS.gene050319 | MS.gene92548 | -0.824782 | 6.70E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene92548 | -0.886183 | 4.27E-72 | -1.69E-46 |
| MS.gene050438 | MS.gene92548 | 0.825226 | 5.26E-54 | -1.69E-46 |
| MS.gene050442 | MS.gene92548 | 0.841869 | 3.66E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene92548 | 0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene050529 | MS.gene92548 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene050558 | MS.gene92548 | 0.873899 | 1.03E-67 | -1.69E-46 |
| MS.gene050623 | MS.gene92548 | -0.891845 | 2.74E-74 | -1.69E-46 |
| MS.gene050642 | MS.gene92548 | 0.880663 | 4.56E-70 | -1.69E-46 |
| MS.gene050719 | MS.gene92548 | 0.853137 | 2.91E-61 | -1.69E-46 |
| MS.gene050721 | MS.gene92548 | 0.852353 | 4.88E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene92548 | 0.852509 | 4.41E-61 | -1.69E-46 |
| MS.gene050750 | MS.gene92548 | 0.816229 | 6.16E-52 | -1.69E-46 |
| MS.gene050762 | MS.gene92548 | 0.824174 | 9.31E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene92548 | 0.826579 | 2.51E-54 | -1.69E-46 |
| MS.gene050787 | MS.gene92548 | 0.818779 | 1.64E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene92548 | 0.802489 | 5.48E-49 | -1.69E-46 |
| MS.gene050820 | MS.gene92548 | 0.84432 | 8.13E-59 | -1.69E-46 |
| MS.gene050824 | MS.gene92548 | 0.841282 | 5.22E-58 | -1.69E-46 |
| MS.gene050953 | MS.gene92548 | 0.822628 | 2.14E-53 | -1.69E-46 |
| MS.gene050987 | MS.gene92548 | 0.809694 | 1.67E-50 | -1.69E-46 |
| MS.gene05119 | MS.gene92548 | -0.874292 | 7.57E-68 | -1.69E-46 |
| MS.gene051257 | MS.gene92548 | 0.843403 | 1.43E-58 | -1.69E-46 |
| MS.gene051349 | MS.gene92548 | 0.843178 | 1.64E-58 | -1.69E-46 |
| MS.gene051535 | MS.gene92548 | 0.81655 | 5.22E-52 | -1.69E-46 |
| MS.gene051633 | MS.gene92548 | 0.808931 | 2.43E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene92548 | 0.861385 | 1.06E-63 | -1.69E-46 |
| MS.gene051663 | MS.gene92548 | 0.857101 | 2.05E-62 | -1.69E-46 |
| MS.gene051860 | MS.gene92548 | 0.86067 | 1.76E-63 | -1.69E-46 |
| MS.gene051983 | MS.gene92548 | 0.878643 | 2.38E-69 | -1.69E-46 |
| MS.gene052048 | MS.gene92548 | -0.809061 | 2.28E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene92548 | 0.859164 | 5.00E-63 | -1.69E-46 |
| MS.gene052176 | MS.gene92548 | 0.892182 | 2.01E-74 | -1.69E-46 |
| MS.gene052178 | MS.gene92548 | 0.804855 | 1.77E-49 | -1.69E-46 |
| MS.gene052256 | MS.gene92548 | 0.811339 | 7.36E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene92548 | 0.806903 | 6.56E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene92548 | 0.869294 | 3.45E-66 | -1.69E-46 |
| MS.gene052309 | MS.gene92548 | -0.887285 | 1.63E-72 | -1.69E-46 |
| MS.gene052340 | MS.gene92548 | 0.811525 | 6.70E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene92548 | -0.816294 | 5.96E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene92548 | 0.882176 | 1.30E-70 | -1.69E-46 |
| MS.gene052389 | MS.gene92548 | 0.839631 | 1.41E-57 | -1.69E-46 |
| MS.gene052420 | MS.gene92548 | 0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene92548 | 0.81772 | 2.85E-52 | -1.69E-46 |
| MS.gene052600 | MS.gene92548 | 0.835379 | 1.74E-56 | -1.69E-46 |
| MS.gene052856 | MS.gene92548 | 0.835718 | 1.43E-56 | -1.69E-46 |
| MS.gene052858 | MS.gene92548 | 0.836593 | 8.55E-57 | -1.69E-46 |
| MS.gene052860 | MS.gene92548 | 0.838794 | 2.33E-57 | -1.69E-46 |
| MS.gene053054 | MS.gene92548 | -0.808503 | 3.00E-50 | -1.69E-46 |
| MS.gene053184 | MS.gene92548 | 0.809488 | 1.85E-50 | -1.69E-46 |
| MS.gene053216 | MS.gene92548 | 0.833508 | 5.12E-56 | -1.69E-46 |
| MS.gene053630 | MS.gene92548 | 0.829792 | 4.22E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene92548 | 0.918194 | 2.10E-86 | -1.69E-46 |
| MS.gene053907 | MS.gene92548 | 0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene054083 | MS.gene92548 | 0.806181 | 9.32E-50 | -1.69E-46 |
| MS.gene054379 | MS.gene92548 | 0.842895 | 1.96E-58 | -1.69E-46 |
| MS.gene054405 | MS.gene92548 | 0.80817 | 3.53E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene92548 | 0.815157 | 1.07E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene92548 | 0.848655 | 5.34E-60 | -1.69E-46 |
| MS.gene054542 | MS.gene92548 | 0.841147 | 5.67E-58 | -1.69E-46 |
| MS.gene054667 | MS.gene92548 | 0.835146 | 1.99E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene92548 | 0.91119 | 8.05E-83 | -1.69E-46 |
| MS.gene054942 | MS.gene92548 | 0.869503 | 2.95E-66 | -1.69E-46 |
| MS.gene054983 | MS.gene92548 | 0.870208 | 1.74E-66 | -1.69E-46 |
| MS.gene05510 | MS.gene92548 | 0.809319 | 2.01E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene92548 | 0.807773 | 4.29E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene92548 | 0.801105 | 1.05E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene92548 | 0.864512 | 1.15E-64 | -1.69E-46 |
| MS.gene055428 | MS.gene92548 | 0.85537 | 6.60E-62 | -1.69E-46 |
| MS.gene05553 | MS.gene92548 | 0.802406 | 5.69E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene92548 | 0.815094 | 1.10E-51 | -1.69E-46 |
| MS.gene055712 | MS.gene92548 | 0.81732 | 3.51E-52 | -1.69E-46 |
| MS.gene055816 | MS.gene92548 | -0.80116 | 1.03E-48 | -1.69E-46 |
| MS.gene055859 | MS.gene92548 | 0.80511 | 1.56E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene92548 | -0.848746 | 5.04E-60 | -1.69E-46 |
| MS.gene056087 | MS.gene92548 | 0.81527 | 1.01E-51 | -1.69E-46 |
| MS.gene056400 | MS.gene92548 | 0.801047 | 1.08E-48 | -1.69E-46 |
| MS.gene056447 | MS.gene92548 | 0.801035 | 1.09E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene92548 | 0.806534 | 7.85E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene92548 | 0.826584 | 2.50E-54 | -1.69E-46 |
| MS.gene056875 | MS.gene92548 | 0.857287 | 1.81E-62 | -1.69E-46 |
| MS.gene056979 | MS.gene92548 | 0.873233 | 1.72E-67 | -1.69E-46 |
| MS.gene05702 | MS.gene92548 | 0.815908 | 7.27E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene92548 | 0.800173 | 1.63E-48 | -1.69E-46 |
| MS.gene057079 | MS.gene92548 | 0.828561 | 8.40E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene92548 | 0.862709 | 4.18E-64 | -1.69E-46 |
| MS.gene057200 | MS.gene92548 | 0.806072 | 9.82E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene92548 | 0.837536 | 4.91E-57 | -1.69E-46 |
| MS.gene057260 | MS.gene92548 | 0.846684 | 1.86E-59 | -1.69E-46 |
| MS.gene057439 | MS.gene92548 | 0.846857 | 1.67E-59 | -1.69E-46 |
| MS.gene057469 | MS.gene92548 | 0.805374 | 1.38E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene92548 | 0.827002 | 1.99E-54 | -1.69E-46 |
| MS.gene057639 | MS.gene92548 | 0.809954 | 1.47E-50 | -1.69E-46 |
| MS.gene057750 | MS.gene92548 | 0.823392 | 1.42E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene92548 | 0.821791 | 3.35E-53 | -1.69E-46 |
| MS.gene05795 | MS.gene92548 | 0.808021 | 3.80E-50 | -1.69E-46 |
| MS.gene057967 | MS.gene92548 | 0.84524 | 4.60E-59 | -1.69E-46 |
| MS.gene058002 | MS.gene92548 | 0.84459 | 6.88E-59 | -1.69E-46 |
| MS.gene058084 | MS.gene92548 | 0.801834 | 7.47E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene92548 | 0.838983 | 2.08E-57 | -1.69E-46 |
| MS.gene058139 | MS.gene92548 | 0.825328 | 4.98E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene92548 | 0.852364 | 4.85E-61 | -1.69E-46 |
| MS.gene058146 | MS.gene92548 | 0.803135 | 4.03E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene92548 | 0.853841 | 1.83E-61 | -1.69E-46 |
| MS.gene058200 | MS.gene92548 | 0.814051 | 1.88E-51 | -1.69E-46 |
| MS.gene058399 | MS.gene92548 | 0.83082 | 2.37E-55 | -1.69E-46 |
| MS.gene058435 | MS.gene92548 | 0.821207 | 4.57E-53 | -1.69E-46 |
| MS.gene058661 | MS.gene92548 | -0.825097 | 5.64E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene92548 | 0.847603 | 1.04E-59 | -1.69E-46 |
| MS.gene058749 | MS.gene92548 | 0.874083 | 8.91E-68 | -1.69E-46 |
| MS.gene058833 | MS.gene92548 | 0.826008 | 3.43E-54 | -1.69E-46 |
| MS.gene058845 | MS.gene92548 | 0.850648 | 1.48E-60 | -1.69E-46 |
| MS.gene058906 | MS.gene92548 | 0.806763 | 7.02E-50 | -1.69E-46 |
| MS.gene059033 | MS.gene92548 | -0.843943 | 1.03E-58 | -1.69E-46 |
| MS.gene05907 | MS.gene92548 | 0.824676 | 7.09E-54 | -1.69E-46 |
| MS.gene059124 | MS.gene92548 | 0.807672 | 4.51E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene92548 | 0.823645 | 1.24E-53 | -1.69E-46 |
| MS.gene059247 | MS.gene92548 | 0.804126 | 2.51E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene92548 | 0.822904 | 1.85E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene92548 | 0.836084 | 1.15E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene92548 | 0.820043 | 8.45E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene92548 | 0.801284 | 9.68E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene92548 | 0.822747 | 2.01E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene92548 | 0.875742 | 2.42E-68 | -1.69E-46 |
| MS.gene059894 | MS.gene92548 | 0.851095 | 1.11E-60 | -1.69E-46 |
| MS.gene059899 | MS.gene92548 | 0.867214 | 1.61E-65 | -1.69E-46 |
| MS.gene060084 | MS.gene92548 | 0.838024 | 3.68E-57 | -1.69E-46 |
| MS.gene060137 | MS.gene92548 | -0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene92548 | 0.834697 | 2.58E-56 | -1.69E-46 |
| MS.gene060322 | MS.gene92548 | 0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene92548 | 0.803871 | 2.83E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene92548 | 0.842068 | 3.24E-58 | -1.69E-46 |
| MS.gene060480 | MS.gene92548 | 0.82176 | 3.40E-53 | -1.69E-46 |
| MS.gene060572 | MS.gene92548 | 0.806454 | 8.16E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene92548 | 0.81638 | 5.70E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene92548 | 0.809156 | 2.18E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene92548 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene92548 | 0.815545 | 8.75E-52 | -1.69E-46 |
| MS.gene060918 | MS.gene92548 | 0.800886 | 1.17E-48 | -1.69E-46 |
| MS.gene060940 | MS.gene92548 | 0.80157 | 8.46E-49 | -1.69E-46 |
| MS.gene060942 | MS.gene92548 | 0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene92548 | 0.822461 | 2.34E-53 | -1.69E-46 |
| MS.gene061002 | MS.gene92548 | 0.800312 | 1.53E-48 | -1.69E-46 |
| MS.gene061040 | MS.gene92548 | -0.867444 | 1.36E-65 | -1.69E-46 |
| MS.gene061049 | MS.gene92548 | 0.869687 | 2.57E-66 | -1.69E-46 |
| MS.gene061085 | MS.gene92548 | 0.814053 | 1.87E-51 | -1.69E-46 |
| MS.gene061113 | MS.gene92548 | 0.809441 | 1.89E-50 | -1.69E-46 |
| MS.gene061114 | MS.gene92548 | -0.831199 | 1.91E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92548.t1 | MTR_4g019910 | 98.431 | 255 | 4 | 0 | 1 | 255 | 1 | 255 | 0.0 | 529 |
| MS.gene92548.t1 | MTR_8g099610 | 25.610 | 246 | 152 | 6 | 5 | 250 | 57 | 271 | 5.20e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92548.t1 | AT3G09250 | 61.140 | 193 | 69 | 3 | 67 | 254 | 39 | 230 | 9.36e-85 | 253 |
| MS.gene92548.t1 | AT3G09250 | 61.140 | 193 | 69 | 3 | 67 | 254 | 53 | 244 | 1.42e-84 | 253 |
| MS.gene92548.t1 | AT3G09250 | 59.341 | 182 | 68 | 3 | 67 | 243 | 39 | 219 | 3.07e-75 | 229 |
| MS.gene92548.t1 | AT4G10925 | 31.667 | 120 | 78 | 2 | 131 | 250 | 104 | 219 | 5.46e-17 | 77.8 |
| MS.gene92548.t1 | AT4G10925 | 31.667 | 120 | 78 | 2 | 131 | 250 | 104 | 219 | 5.46e-17 | 77.8 |
| MS.gene92548.t1 | AT4G10925 | 31.667 | 120 | 78 | 2 | 131 | 250 | 104 | 219 | 5.46e-17 | 77.8 |
| MS.gene92548.t1 | AT4G10925 | 31.667 | 120 | 78 | 2 | 131 | 250 | 104 | 219 | 5.46e-17 | 77.8 |
| MS.gene92548.t1 | AT4G10925 | 31.667 | 120 | 78 | 2 | 131 | 250 | 104 | 219 | 5.46e-17 | 77.8 |
| MS.gene92548.t1 | AT4G23960 | 29.839 | 124 | 76 | 3 | 135 | 255 | 73 | 188 | 6.04e-12 | 63.2 |
Find 45 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTAACTTATTTGGATTAA+AGG | 0.134555 | 4.2:-82339309 | None:intergenic |
| TTGCCAAAAGCTAACTTATT+TGG | 0.143563 | 4.2:-82339317 | None:intergenic |
| ATGCTAAAGCAGCAGAAATA+AGG | 0.234285 | 4.2:+82339940 | MS.gene92548:CDS |
| GATAGCAACACAGCAGTATT+TGG | 0.251611 | 4.2:+82339987 | MS.gene92548:CDS |
| AATCCAAATAAGTTAGCTTT+TGG | 0.283724 | 4.2:+82339314 | MS.gene92548:CDS |
| CAAGGAGTTCAGTTTCTTGC+CGG | 0.285437 | 4.2:+82339238 | MS.gene92548:CDS |
| ATTAGATGAAAACACTTTAG+AGG | 0.343894 | 4.2:+82339884 | MS.gene92548:CDS |
| TCTTCTAGTTTGATTTGTAA+TGG | 0.367473 | 4.2:-82340179 | None:intergenic |
| GTTCAGTTTCTTGCCGGGTT+CGG | 0.410478 | 4.2:+82339244 | MS.gene92548:CDS |
| TTGAGAGCTGGGATTTCGTA+TGG | 0.419032 | 4.2:+82340141 | MS.gene92548:CDS |
| TGCTAAAGCAGCAGAAATAA+GGG | 0.421596 | 4.2:+82339941 | MS.gene92548:CDS |
| TGCAACTTGAGGCATTGATT+AGG | 0.431017 | 4.2:-82339374 | None:intergenic |
| CCGGATATTCCTTTCATACC+AGG | 0.479450 | 4.2:-82340104 | None:intergenic |
| ATCTTCTCATTTGCAACTTG+AGG | 0.480992 | 4.2:-82339385 | None:intergenic |
| ATGTATCAATTCATATCTAC+TGG | 0.481405 | 4.2:-82340350 | None:intergenic |
| CCTGGTATGAAAGGAATATC+CGG | 0.485068 | 4.2:+82340104 | MS.gene92548:CDS |
| AATGTGTTCGAGAGGATCAA+TGG | 0.487074 | 4.2:+82340296 | MS.gene92548:CDS |
| CTCAATAACATCATCATAGC+CGG | 0.487596 | 4.2:-82340123 | None:intergenic |
| GGCGACCTTGCAGCCATGCA+AGG | 0.495354 | 4.2:+82340044 | MS.gene92548:CDS |
| ATGATGATGTTATTGAGAGC+TGG | 0.502409 | 4.2:+82340129 | MS.gene92548:CDS |
| AAGGAGTTCAGTTTCTTGCC+GGG | 0.510954 | 4.2:+82339239 | MS.gene92548:CDS |
| ACATGCCTTGCATGGCTGCA+AGG | 0.515179 | 4.2:-82340049 | None:intergenic |
| GTTTCTGTCAGGGACACGAC+AGG | 0.525904 | 4.2:+82339812 | MS.gene92548:intron |
| AGTTCATGCTAGAGGGGACA+TGG | 0.526900 | 4.2:+82340208 | MS.gene92548:CDS |
| GACATTAAAGTTCATGCTAG+AGG | 0.527041 | 4.2:+82340200 | MS.gene92548:CDS |
| GGGACTTTCTTCTACTCTCA+AGG | 0.531322 | 4.2:+82339220 | MS.gene92548:intron |
| TTGCAGCCATGCAAGGCATG+TGG | 0.533455 | 4.2:+82340051 | MS.gene92548:CDS |
| TGATGATGTTATTGAGAGCT+GGG | 0.533980 | 4.2:+82340130 | MS.gene92548:CDS |
| ACATTAAAGTTCATGCTAGA+GGG | 0.562565 | 4.2:+82340201 | MS.gene92548:CDS |
| AAGCTATTGATGACCGAACC+CGG | 0.564308 | 4.2:-82339257 | None:intergenic |
| TGCAGCCATGCAAGGCATGT+GGG | 0.568080 | 4.2:+82340052 | MS.gene92548:CDS |
| TGAGAGCTGGGATTTCGTAT+GGG | 0.571314 | 4.2:+82340142 | MS.gene92548:CDS |
| GAGGTATGTTGTGTTCATCC+TGG | 0.582448 | 4.2:+82340086 | MS.gene92548:CDS |
| CATGGGGTATGTTACATGCA+TGG | 0.595261 | 4.2:+82340226 | MS.gene92548:CDS |
| GTTCATGCTAGAGGGGACAT+GGG | 0.595900 | 4.2:+82340209 | MS.gene92548:CDS |
| TTGTAACGAATGTGTTCGAG+AGG | 0.614427 | 4.2:+82340288 | MS.gene92548:CDS |
| TTTCTGTCAGGGACACGACA+GGG | 0.615152 | 4.2:+82339813 | MS.gene92548:intron |
| CATTAAAGTTCATGCTAGAG+GGG | 0.624713 | 4.2:+82340202 | MS.gene92548:CDS |
| TGTGTTCATCCTGGTATGAA+AGG | 0.628945 | 4.2:+82340095 | MS.gene92548:CDS |
| ACGAAAGGAGGAAGATGGGG+AGG | 0.630749 | 4.2:+82340260 | MS.gene92548:CDS |
| AAAACGAAAGGAGGAAGATG+GGG | 0.635971 | 4.2:+82340257 | MS.gene92548:CDS |
| TTCATGCTAGAGGGGACATG+GGG | 0.652035 | 4.2:+82340210 | MS.gene92548:CDS |
| TCGAGAGGATCAATGGCGAG+TGG | 0.664074 | 4.2:+82340303 | MS.gene92548:CDS |
| GCTACAGCATGCAATTGCTG+AGG | 0.668140 | 4.2:+82339911 | MS.gene92548:CDS |
| GGAGAGCATGTTGAGCAGTG+AGG | 0.712787 | 4.2:+82339854 | MS.gene92548:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTCTCTTTTAAGTTTTAA+GGG | + | chr4.2:82338853-82338872 | MS.gene92548:intron | 15.0% |
| !!! | TTTTTTACATAGAATTTCAA+TGG | + | chr4.2:82339024-82339043 | MS.gene92548:intron | 15.0% |
| !! | TAACTTTATAAAATTGCCAT+TGG | + | chr4.2:82339790-82339809 | MS.gene92548:intron | 20.0% |
| !!! | AATTTTGTGTTTTTTCATGA+AGG | + | chr4.2:82339199-82339218 | MS.gene92548:intron | 20.0% |
| !!! | ATTTTGTGTTTTTTCATGAA+GGG | + | chr4.2:82339200-82339219 | MS.gene92548:intron | 20.0% |
| !!! | GAATTCTCTTTTAAGTTTTA+AGG | + | chr4.2:82338852-82338871 | MS.gene92548:intron | 20.0% |
| !!! | TTTGATTTAACTACTTGAAA+TGG | + | chr4.2:82338957-82338976 | MS.gene92548:intron | 20.0% |
| ! | AAAAAAAAAAATCCAACTCC+CGG | - | chr4.2:82339008-82339027 | None:intergenic | 25.0% |
| ! | AAACTACATAATCCTTGAAA+AGG | - | chr4.2:82339142-82339161 | None:intergenic | 25.0% |
| ! | AACTACTTGAAATGGAAATT+TGG | + | chr4.2:82338965-82338984 | MS.gene92548:intron | 25.0% |
| ! | AGCTAACTTATTTGGATTAA+AGG | - | chr4.2:82339312-82339331 | None:intergenic | 25.0% |
| ! | AGTACCAATAAGAATCAAAA+GGG | - | chr4.2:82339434-82339453 | None:intergenic | 25.0% |
| ! | ATAGTTAGTACAGAAAACTT+AGG | - | chr4.2:82339570-82339589 | None:intergenic | 25.0% |
| ! | ATCAAGACTATAGAAAGAAA+TGG | - | chr4.2:82339513-82339532 | None:intergenic | 25.0% |
| ! | ATCTATCGAATTCAGAATTT+CGG | + | chr4.2:82338921-82338940 | MS.gene92548:intron | 25.0% |
| ! | ATGGAATTTGTAAAAACGAA+AGG | + | chr4.2:82340245-82340264 | MS.gene92548:CDS | 25.0% |
| ! | ATTAGATGAAAACACTTTAG+AGG | + | chr4.2:82339884-82339903 | MS.gene92548:CDS | 25.0% |
| ! | CTTCAGTGAAATTCATAAAA+AGG | - | chr4.2:82339070-82339089 | None:intergenic | 25.0% |
| ! | CTTTGTACTAAATAGTGTAT+CGG | - | chr4.2:82339683-82339702 | None:intergenic | 25.0% |
| ! | TACATAGAATTTCAATGGAA+TGG | + | chr4.2:82339029-82339048 | MS.gene92548:intron | 25.0% |
| ! | TAGTACCAATAAGAATCAAA+AGG | - | chr4.2:82339435-82339454 | None:intergenic | 25.0% |
| ! | TTTCTGCAAAATTATCACTT+AGG | + | chr4.2:82338876-82338895 | MS.gene92548:intron | 25.0% |
| ! | TTTGTACTAAATAGTGTATC+GGG | - | chr4.2:82339682-82339701 | None:intergenic | 25.0% |
| !! | AATTTAAAAATTTGCTCACG+TGG | + | chr4.2:82339164-82339183 | MS.gene92548:intron | 25.0% |
| !! | TTTTATGATTCATTCAGGAA+TGG | + | chr4.2:82340023-82340042 | MS.gene92548:CDS | 25.0% |
| !!! | AATCCAAATAAGTTAGCTTT+TGG | + | chr4.2:82339314-82339333 | MS.gene92548:CDS | 25.0% |
| !!! | ATAAGTGTAATTCTGAAACA+AGG | - | chr4.2:82338813-82338832 | None:intergenic | 25.0% |
| !!! | TAAGTGTAATTCTGAAACAA+GGG | - | chr4.2:82338812-82338831 | None:intergenic | 25.0% |
| !!! | TACAAAGTACTGTCAAATAA+CGG | + | chr4.2:82339696-82339715 | MS.gene92548:intron | 25.0% |
| !!! | TCTTCTAGTTTGATTTGTAA+TGG | - | chr4.2:82340182-82340201 | None:intergenic | 25.0% |
| !!! | TTCTTATTGGTACTACTTTT+TGG | + | chr4.2:82339440-82339459 | MS.gene92548:intron | 25.0% |
| ACATTAAAGTTCATGCTAGA+GGG | + | chr4.2:82340201-82340220 | MS.gene92548:CDS | 30.0% | |
| AGCCTAGATGCAAATATAAT+AGG | - | chr4.2:82339547-82339566 | None:intergenic | 30.0% | |
| CTTGAAATGGAAATTTGGAA+TGG | + | chr4.2:82338970-82338989 | MS.gene92548:intron | 30.0% | |
| GAATTTGTAAAAACGAAAGG+AGG | + | chr4.2:82340248-82340267 | MS.gene92548:CDS | 30.0% | |
| GTCCTATTATATTTGCATCT+AGG | + | chr4.2:82339542-82339561 | MS.gene92548:intron | 30.0% | |
| TAGAATTTCAATGGAATGGT+TGG | + | chr4.2:82339033-82339052 | MS.gene92548:intron | 30.0% | |
| TCTATCGAATTCAGAATTTC+GGG | + | chr4.2:82338922-82338941 | MS.gene92548:intron | 30.0% | |
| TTGCCAAAAGCTAACTTATT+TGG | - | chr4.2:82339320-82339339 | None:intergenic | 30.0% | |
| TTGTTAATTGCGAAGAATAG+AGG | - | chr4.2:82339767-82339786 | None:intergenic | 30.0% | |
| ! | CGATATGTTTTTCCTTTTCA+AGG | + | chr4.2:82339127-82339146 | MS.gene92548:intron | 30.0% |
| ! | TTATAAGTGTCGTCAAATAG+CGG | + | chr4.2:82339613-82339632 | MS.gene92548:intron | 30.0% |
| !! | GCAAGTTTTATGATTCATTC+AGG | + | chr4.2:82340018-82340037 | MS.gene92548:CDS | 30.0% |
| !!! | TTTGGCAAAAGTTTTGAAAG+AGG | + | chr4.2:82339332-82339351 | MS.gene92548:CDS | 30.0% |
| AAAAACGAAAGGAGGAAGAT+GGG | + | chr4.2:82340256-82340275 | MS.gene92548:CDS | 35.0% | |
| AAGAAGTCTCACTTCATGAA+TGG | + | chr4.2:82339087-82339106 | MS.gene92548:intron | 35.0% | |
| ATAGTGTATCGGGATAATAG+TGG | - | chr4.2:82339672-82339691 | None:intergenic | 35.0% | |
| ATCTTCTCATTTGCAACTTG+AGG | - | chr4.2:82339388-82339407 | None:intergenic | 35.0% | |
| ATGCTAAAGCAGCAGAAATA+AGG | + | chr4.2:82339940-82339959 | MS.gene92548:CDS | 35.0% | |
| CATTAAAGTTCATGCTAGAG+GGG | + | chr4.2:82340202-82340221 | MS.gene92548:CDS | 35.0% | |
| CTCAATAACATCATCATAGC+CGG | - | chr4.2:82340126-82340145 | None:intergenic | 35.0% | |
| GACATTAAAGTTCATGCTAG+AGG | + | chr4.2:82340200-82340219 | MS.gene92548:CDS | 35.0% | |
| TAAAAACGAAAGGAGGAAGA+TGG | + | chr4.2:82340255-82340274 | MS.gene92548:CDS | 35.0% | |
| TCATGAATGGTTGAGGAATA+AGG | + | chr4.2:82339100-82339119 | MS.gene92548:intron | 35.0% | |
| TGCTAAAGCAGCAGAAATAA+GGG | + | chr4.2:82339941-82339960 | MS.gene92548:CDS | 35.0% | |
| ! | ATGATGATGTTATTGAGAGC+TGG | + | chr4.2:82340129-82340148 | MS.gene92548:CDS | 35.0% |
| ! | TGATGATGTTATTGAGAGCT+GGG | + | chr4.2:82340130-82340149 | MS.gene92548:CDS | 35.0% |
| !! | CGAACCCTTTTGATTCTTAT+TGG | + | chr4.2:82339427-82339446 | MS.gene92548:intron | 35.0% |
| !! | CTGTGTTGCTATCTTTTTGT+AGG | - | chr4.2:82339980-82339999 | None:intergenic | 35.0% |
| AAAAAAAATCCAACTCCCGG+AGG | - | chr4.2:82339005-82339024 | None:intergenic | 40.0% | |
| AAAACGAAAGGAGGAAGATG+GGG | + | chr4.2:82340257-82340276 | MS.gene92548:CDS | 40.0% | |
| AATCAAAAGGGTTCGAGCAA+AGG | - | chr4.2:82339422-82339441 | None:intergenic | 40.0% | |
| AATGTGTTCGAGAGGATCAA+TGG | + | chr4.2:82340296-82340315 | MS.gene92548:CDS | 40.0% | |
| CCTGGTATGAAAGGAATATC+CGG | + | chr4.2:82340104-82340123 | MS.gene92548:CDS | 40.0% | |
| GAATGGTTTATAACTCCTCC+GGG | + | chr4.2:82338987-82339006 | MS.gene92548:intron | 40.0% | |
| GATAGCAACACAGCAGTATT+TGG | + | chr4.2:82339987-82340006 | MS.gene92548:CDS | 40.0% | |
| GGAATGGTTTATAACTCCTC+CGG | + | chr4.2:82338986-82339005 | MS.gene92548:intron | 40.0% | |
| TCTCACTTCATGAATGGTTG+AGG | + | chr4.2:82339093-82339112 | MS.gene92548:intron | 40.0% | |
| TGTGTTCATCCTGGTATGAA+AGG | + | chr4.2:82340095-82340114 | MS.gene92548:CDS | 40.0% | |
| TTGTAACGAATGTGTTCGAG+AGG | + | chr4.2:82340288-82340307 | MS.gene92548:CDS | 40.0% | |
| ! | AAATTTGCTCACGTGGTTCA+TGG | + | chr4.2:82339171-82339190 | MS.gene92548:intron | 40.0% |
| !! | AATTGCCATTGGTTTCTGTC+AGG | + | chr4.2:82339801-82339820 | MS.gene92548:intron | 40.0% |
| !! | ATTGCCATTGGTTTCTGTCA+GGG | + | chr4.2:82339802-82339821 | MS.gene92548:intron | 40.0% |
| !! | TGCAACTTGAGGCATTGATT+AGG | - | chr4.2:82339377-82339396 | None:intergenic | 40.0% |
| AAGGAGTTCAGTTTCTTGCC+GGG | + | chr4.2:82339239-82339258 | MS.gene92548:CDS | 45.0% | |
| ATTTCGCTATGCTACAGTGC+TGG | - | chr4.2:82339735-82339754 | None:intergenic | 45.0% | |
| CAAGGAGTTCAGTTTCTTGC+CGG | + | chr4.2:82339238-82339257 | MS.gene92548:CDS | 45.0% | |
| CATGGGGTATGTTACATGCA+TGG | + | chr4.2:82340226-82340245 | MS.gene92548:CDS | 45.0% | |
| CCGGATATTCCTTTCATACC+AGG | - | chr4.2:82340107-82340126 | None:intergenic | 45.0% | |
| GAGGTATGTTGTGTTCATCC+TGG | + | chr4.2:82340086-82340105 | MS.gene92548:CDS | 45.0% | |
| GGGACTTTCTTCTACTCTCA+AGG | + | chr4.2:82339220-82339239 | MS.gene92548:intron | 45.0% | |
| GGGTGTAAAAAGTGAAGACG+CGG | + | chr4.2:82339833-82339852 | MS.gene92548:CDS | 45.0% | |
| TGAGAGCTGGGATTTCGTAT+GGG | + | chr4.2:82340142-82340161 | MS.gene92548:CDS | 45.0% | |
| TTGAGAGCTGGGATTTCGTA+TGG | + | chr4.2:82340141-82340160 | MS.gene92548:CDS | 45.0% | |
| TTTATAACTCCTCCGGGAGT+TGG | + | chr4.2:82338993-82339012 | MS.gene92548:intron | 45.0% | |
| ! | AAGCTATTGATGACCGAACC+CGG | - | chr4.2:82339260-82339279 | None:intergenic | 45.0% |
| AGTTCATGCTAGAGGGGACA+TGG | + | chr4.2:82340208-82340227 | MS.gene92548:CDS | 50.0% | |
| GCAAGGCATGTGGGCAAAAA+CGG | + | chr4.2:82340061-82340080 | MS.gene92548:CDS | 50.0% | |
| GCTACAGCATGCAATTGCTG+AGG | + | chr4.2:82339911-82339930 | MS.gene92548:CDS | 50.0% | |
| GTGTCCCTGACAGAAACCAA+TGG | - | chr4.2:82339809-82339828 | None:intergenic | 50.0% | |
| GTTCAGTTTCTTGCCGGGTT+CGG | + | chr4.2:82339244-82339263 | MS.gene92548:CDS | 50.0% | |
| GTTCATGCTAGAGGGGACAT+GGG | + | chr4.2:82340209-82340228 | MS.gene92548:CDS | 50.0% | |
| TTCATGCTAGAGGGGACATG+GGG | + | chr4.2:82340210-82340229 | MS.gene92548:CDS | 50.0% | |
| TTTCTGTCAGGGACACGACA+GGG | + | chr4.2:82339813-82339832 | MS.gene92548:intron | 50.0% | |
| ! | TTTTGCCCACATGCCTTGCA+TGG | - | chr4.2:82340060-82340079 | None:intergenic | 50.0% |
| ACATGCCTTGCATGGCTGCA+AGG | - | chr4.2:82340052-82340071 | None:intergenic | 55.0% | |
| ACGAAAGGAGGAAGATGGGG+AGG | + | chr4.2:82340260-82340279 | MS.gene92548:CDS | 55.0% | |
| CATGTGGGCAAAAACGGACG+AGG | + | chr4.2:82340067-82340086 | MS.gene92548:CDS | 55.0% | |
| GTTTCTGTCAGGGACACGAC+AGG | + | chr4.2:82339812-82339831 | MS.gene92548:intron | 55.0% | |
| TCGAGAGGATCAATGGCGAG+TGG | + | chr4.2:82340303-82340322 | MS.gene92548:CDS | 55.0% | |
| TGCAGCCATGCAAGGCATGT+GGG | + | chr4.2:82340052-82340071 | MS.gene92548:CDS | 55.0% | |
| TTGCAGCCATGCAAGGCATG+TGG | + | chr4.2:82340051-82340070 | MS.gene92548:CDS | 55.0% | |
| ! | GGAGAGCATGTTGAGCAGTG+AGG | + | chr4.2:82339854-82339873 | MS.gene92548:CDS | 55.0% |
| GGCGACCTTGCAGCCATGCA+AGG | + | chr4.2:82340044-82340063 | MS.gene92548:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 82338768 | 82340367 | 82338768 | ID=MS.gene92548 |
| chr4.2 | mRNA | 82338768 | 82340367 | 82338768 | ID=MS.gene92548.t1;Parent=MS.gene92548 |
| chr4.2 | exon | 82338768 | 82338800 | 82338768 | ID=MS.gene92548.t1.exon1;Parent=MS.gene92548.t1 |
| chr4.2 | CDS | 82338768 | 82338800 | 82338768 | ID=cds.MS.gene92548.t1;Parent=MS.gene92548.t1 |
| chr4.2 | exon | 82339221 | 82339410 | 82339221 | ID=MS.gene92548.t1.exon2;Parent=MS.gene92548.t1 |
| chr4.2 | CDS | 82339221 | 82339410 | 82339221 | ID=cds.MS.gene92548.t1;Parent=MS.gene92548.t1 |
| chr4.2 | exon | 82339823 | 82340367 | 82339823 | ID=MS.gene92548.t1.exon3;Parent=MS.gene92548.t1 |
| chr4.2 | CDS | 82339823 | 82340367 | 82339823 | ID=cds.MS.gene92548.t1;Parent=MS.gene92548.t1 |
| Gene Sequence |
| Protein sequence |
