Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77584.t1 | XP_003601765.1 | 83.1 | 415 | 12 | 3 | 4 | 361 | 52 | 465 | 5.10E-184 | 653.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77584.t1 | Q9FZK1 | 56.5 | 416 | 123 | 5 | 4 | 361 | 52 | 467 | 7.3e-131 | 468.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77584.t1 | G7JBG1 | 83.1 | 415 | 12 | 3 | 4 | 361 | 52 | 465 | 3.7e-184 | 653.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049195 | MS.gene77584 | -0.843255 | 1.57E-58 | -1.69E-46 |
| MS.gene049297 | MS.gene77584 | -0.844422 | 7.64E-59 | -1.69E-46 |
| MS.gene04933 | MS.gene77584 | -0.847304 | 1.26E-59 | -1.69E-46 |
| MS.gene049378 | MS.gene77584 | -0.870388 | 1.52E-66 | -1.69E-46 |
| MS.gene049599 | MS.gene77584 | 0.81449 | 1.50E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene77584 | -0.851678 | 7.59E-61 | -1.69E-46 |
| MS.gene049910 | MS.gene77584 | -0.815238 | 1.02E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene77584 | -0.84834 | 6.52E-60 | -1.69E-46 |
| MS.gene050227 | MS.gene77584 | -0.867478 | 1.33E-65 | -1.69E-46 |
| MS.gene050228 | MS.gene77584 | -0.858083 | 1.05E-62 | -1.69E-46 |
| MS.gene050319 | MS.gene77584 | 0.807029 | 6.17E-50 | -1.69E-46 |
| MS.gene050330 | MS.gene77584 | 0.875129 | 3.93E-68 | -1.69E-46 |
| MS.gene050397 | MS.gene77584 | -0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene050558 | MS.gene77584 | -0.838638 | 2.55E-57 | -1.69E-46 |
| MS.gene050623 | MS.gene77584 | 0.873212 | 1.75E-67 | -1.69E-46 |
| MS.gene050642 | MS.gene77584 | -0.854699 | 1.03E-61 | -1.69E-46 |
| MS.gene050787 | MS.gene77584 | -0.827521 | 1.49E-54 | -1.69E-46 |
| MS.gene050820 | MS.gene77584 | -0.812254 | 4.65E-51 | -1.69E-46 |
| MS.gene05119 | MS.gene77584 | 0.842533 | 2.44E-58 | -1.69E-46 |
| MS.gene051257 | MS.gene77584 | -0.830428 | 2.95E-55 | -1.69E-46 |
| MS.gene051662 | MS.gene77584 | -0.830163 | 3.43E-55 | -1.69E-46 |
| MS.gene051663 | MS.gene77584 | -0.826418 | 2.74E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene77584 | -0.841401 | 4.86E-58 | -1.69E-46 |
| MS.gene051983 | MS.gene77584 | -0.838131 | 3.45E-57 | -1.69E-46 |
| MS.gene052011 | MS.gene77584 | 0.820768 | 5.76E-53 | -1.69E-46 |
| MS.gene052048 | MS.gene77584 | 0.80424 | 2.38E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene77584 | -0.848216 | 7.06E-60 | -1.69E-46 |
| MS.gene052176 | MS.gene77584 | -0.880445 | 5.46E-70 | -1.69E-46 |
| MS.gene052302 | MS.gene77584 | -0.813554 | 2.41E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene77584 | 0.861069 | 1.33E-63 | -1.69E-46 |
| MS.gene052358 | MS.gene77584 | 0.851822 | 6.91E-61 | -1.69E-46 |
| MS.gene052361 | MS.gene77584 | 0.807205 | 5.66E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene77584 | -0.824091 | 9.74E-54 | -1.69E-46 |
| MS.gene052389 | MS.gene77584 | -0.842808 | 2.06E-58 | -1.69E-46 |
| MS.gene052420 | MS.gene77584 | -0.823865 | 1.10E-53 | -1.69E-46 |
| MS.gene052600 | MS.gene77584 | -0.825698 | 4.07E-54 | -1.69E-46 |
| MS.gene053054 | MS.gene77584 | 0.83687 | 7.26E-57 | -1.69E-46 |
| MS.gene053811 | MS.gene77584 | -0.81252 | 4.07E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene77584 | -0.890355 | 1.06E-73 | -1.69E-46 |
| MS.gene053907 | MS.gene77584 | -0.801427 | 9.05E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene77584 | -0.802947 | 4.41E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene77584 | -0.813145 | 2.97E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene77584 | -0.828605 | 8.19E-55 | -1.69E-46 |
| MS.gene054667 | MS.gene77584 | -0.804329 | 2.28E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene77584 | -0.844456 | 7.48E-59 | -1.69E-46 |
| MS.gene054942 | MS.gene77584 | -0.857642 | 1.42E-62 | -1.69E-46 |
| MS.gene054983 | MS.gene77584 | -0.846808 | 1.72E-59 | -1.69E-46 |
| MS.gene055277 | MS.gene77584 | -0.825827 | 3.79E-54 | -1.69E-46 |
| MS.gene055422 | MS.gene77584 | -0.821012 | 5.06E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene77584 | -0.845183 | 4.76E-59 | -1.69E-46 |
| MS.gene05553 | MS.gene77584 | -0.804991 | 1.66E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene77584 | -0.80418 | 2.44E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene77584 | 0.832095 | 1.15E-55 | -1.69E-46 |
| MS.gene056087 | MS.gene77584 | -0.806109 | 9.65E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene77584 | -0.822149 | 2.76E-53 | -1.69E-46 |
| MS.gene056875 | MS.gene77584 | -0.815863 | 7.43E-52 | -1.69E-46 |
| MS.gene056979 | MS.gene77584 | -0.828861 | 7.10E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene77584 | -0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene77584 | -0.836035 | 1.18E-56 | -1.69E-46 |
| MS.gene057245 | MS.gene77584 | -0.814442 | 1.54E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene77584 | -0.827429 | 1.57E-54 | -1.69E-46 |
| MS.gene057967 | MS.gene77584 | -0.81991 | 9.06E-53 | -1.69E-46 |
| MS.gene05811 | MS.gene77584 | -0.824484 | 7.87E-54 | -1.69E-46 |
| MS.gene058139 | MS.gene77584 | -0.805289 | 1.43E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene77584 | -0.805477 | 1.31E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene77584 | -0.810469 | 1.13E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene77584 | -0.80561 | 1.23E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene77584 | -0.826984 | 2.01E-54 | -1.69E-46 |
| MS.gene058749 | MS.gene77584 | -0.812814 | 3.51E-51 | -1.69E-46 |
| MS.gene059033 | MS.gene77584 | 0.865965 | 4.03E-65 | -1.69E-46 |
| MS.gene05907 | MS.gene77584 | -0.818366 | 2.04E-52 | -1.69E-46 |
| MS.gene05984 | MS.gene77584 | -0.843593 | 1.27E-58 | -1.69E-46 |
| MS.gene059894 | MS.gene77584 | -0.810427 | 1.16E-50 | -1.69E-46 |
| MS.gene060137 | MS.gene77584 | 0.813068 | 3.09E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene77584 | -0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene77584 | -0.815533 | 8.81E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene77584 | 0.868227 | 7.64E-66 | -1.69E-46 |
| MS.gene061049 | MS.gene77584 | -0.845665 | 3.53E-59 | -1.69E-46 |
| MS.gene061114 | MS.gene77584 | 0.837699 | 4.46E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77584.t1 | MTR_3g085160 | 83.092 | 414 | 14 | 2 | 4 | 361 | 52 | 465 | 0.0 | 689 |
| MS.gene77584.t1 | MTR_3g085160 | 82.323 | 396 | 14 | 2 | 22 | 361 | 1 | 396 | 0.0 | 647 |
| MS.gene77584.t1 | MTR_1g033310 | 40.720 | 361 | 149 | 11 | 52 | 360 | 2 | 349 | 5.76e-74 | 233 |
| MS.gene77584.t1 | MTR_1g033280 | 38.544 | 371 | 149 | 10 | 57 | 360 | 18 | 376 | 6.21e-72 | 229 |
| MS.gene77584.t1 | MTR_7g017400 | 28.045 | 353 | 190 | 14 | 43 | 335 | 68 | 416 | 7.32e-24 | 102 |
| MS.gene77584.t1 | MTR_1g014700 | 36.800 | 125 | 74 | 3 | 69 | 190 | 20 | 142 | 5.64e-20 | 90.9 |
| MS.gene77584.t1 | MTR_8g028250 | 34.454 | 119 | 75 | 2 | 72 | 189 | 45 | 161 | 1.38e-17 | 84.0 |
| MS.gene77584.t1 | MTR_8g028220 | 33.333 | 120 | 75 | 3 | 71 | 189 | 44 | 159 | 1.48e-15 | 77.8 |
| MS.gene77584.t1 | MTR_4g094748 | 30.952 | 168 | 94 | 5 | 63 | 211 | 55 | 219 | 2.98e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77584.t1 | AT1G27340 | 58.252 | 412 | 112 | 7 | 9 | 361 | 57 | 467 | 1.84e-165 | 471 |
| MS.gene77584.t1 | AT5G15710 | 26.503 | 366 | 199 | 14 | 39 | 338 | 68 | 429 | 4.87e-21 | 94.4 |
| MS.gene77584.t1 | AT5G15710 | 26.503 | 366 | 199 | 14 | 39 | 338 | 68 | 429 | 4.87e-21 | 94.4 |
| MS.gene77584.t1 | AT4G33160 | 29.560 | 159 | 88 | 3 | 51 | 192 | 42 | 193 | 3.25e-18 | 85.9 |
| MS.gene77584.t1 | AT4G33160 | 29.560 | 159 | 88 | 3 | 51 | 192 | 103 | 254 | 5.50e-18 | 85.5 |
| MS.gene77584.t1 | AT3G61590 | 36.667 | 120 | 72 | 3 | 72 | 189 | 52 | 169 | 3.49e-17 | 82.8 |
| MS.gene77584.t1 | AT3G61590 | 36.667 | 120 | 72 | 3 | 72 | 189 | 52 | 169 | 3.49e-17 | 82.8 |
| MS.gene77584.t1 | AT3G61590 | 36.667 | 120 | 72 | 3 | 72 | 189 | 44 | 161 | 3.68e-17 | 82.8 |
| MS.gene77584.t1 | AT3G61590 | 36.667 | 120 | 72 | 3 | 72 | 189 | 80 | 197 | 4.53e-17 | 82.4 |
| MS.gene77584.t1 | AT1G30950 | 30.178 | 169 | 95 | 5 | 63 | 211 | 42 | 207 | 2.81e-13 | 71.2 |
Find 97 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTCCAAGCACCATATATT+TGG | 0.306854 | 3.4:+79470045 | None:intergenic |
| AAAGAATGGCTGAAGGTTCC+TGG | 0.339162 | 3.4:-79469916 | MS.gene77584:CDS |
| ACCCATGAAAATGTGAATTC+AGG | 0.349764 | 3.4:-79470691 | MS.gene77584:CDS |
| AAGTTATGGAACAAGAAATA+TGG | 0.365064 | 3.4:-79470882 | MS.gene77584:CDS |
| TTCCATAACTTCACTTGTAC+TGG | 0.365202 | 3.4:+79470894 | None:intergenic |
| CCGTCAATGGAATTCCTTGC+TGG | 0.368398 | 3.4:-79470779 | MS.gene77584:CDS |
| CACTGCCGTTTCTTCCCATT+CGG | 0.369756 | 3.4:+79469883 | None:intergenic |
| CCCCTGCTTCGATCTCTTCG+AGG | 0.376451 | 3.4:+79470934 | None:intergenic |
| TAGACAGAATGCCAAATATA+TGG | 0.389573 | 3.4:-79470056 | MS.gene77584:CDS |
| TGCCATTGTCCCTTAACTTC+AGG | 0.389987 | 3.4:-79470322 | MS.gene77584:intron |
| ACAGCCTCAAATAAATCTTC+AGG | 0.391468 | 3.4:+79470850 | None:intergenic |
| ATGATATGGCAACAGGGGTT+TGG | 0.394545 | 3.4:-79470232 | MS.gene77584:intron |
| CCCACTAACTCAATCCTTCA+AGG | 0.400080 | 3.4:-79470524 | MS.gene77584:CDS |
| CAGTTTGCGACCTGAAGTTA+AGG | 0.407146 | 3.4:+79470312 | None:intergenic |
| CGAAGCAGGGGCAAATCATC+AGG | 0.419820 | 3.4:-79470922 | MS.gene77584:CDS |
| AATGGCAGGTTCATACTTCC+AGG | 0.420328 | 3.4:+79470338 | None:intergenic |
| GTCAGTCAATGTAGTGGCGC+TGG | 0.428265 | 3.4:+79470194 | None:intergenic |
| ATCCAGTACAAGTGAAGTTA+TGG | 0.433150 | 3.4:-79470896 | MS.gene77584:CDS |
| TCCTTGAAGGATTGAGTTAG+TGG | 0.433327 | 3.4:+79470523 | None:intergenic |
| CCGAGCTGGCAACTCCTTGA+AGG | 0.435297 | 3.4:+79470510 | None:intergenic |
| CAGCCACGTGTGTATGCATA+TGG | 0.440067 | 3.4:-79470113 | MS.gene77584:CDS |
| CGAGGCTTCTTAGCGGGTGG+AGG | 0.447598 | 3.4:+79470952 | None:intergenic |
| TTAGTTTGCTTTCTTGACAT+TGG | 0.450160 | 3.4:-79470571 | MS.gene77584:CDS |
| GTCTGGTCTCGCGTTGCTGT+AGG | 0.453658 | 3.4:-79470478 | MS.gene77584:CDS |
| CCTGGGTGTGTGGTGCCGAA+TGG | 0.455358 | 3.4:-79469898 | MS.gene77584:CDS |
| TGGCAAAACAATCAACTTTG+TGG | 0.460094 | 3.4:+79470612 | None:intergenic |
| CTGGGTGTGTGGTGCCGAAT+GGG | 0.467430 | 3.4:-79469897 | MS.gene77584:CDS |
| GTTGCCAGCTCGGTCAGTTA+AGG | 0.467547 | 3.4:-79470500 | MS.gene77584:CDS |
| TCTGGTCTCGCGTTGCTGTA+GGG | 0.474671 | 3.4:-79470477 | MS.gene77584:CDS |
| TCTCTTCGAGGCTTCTTAGC+GGG | 0.476627 | 3.4:+79470946 | None:intergenic |
| GGCAAAACAATCAACTTTGT+GGG | 0.476939 | 3.4:+79470613 | None:intergenic |
| TCCGCTCTGTATGCCGTCAA+TGG | 0.480162 | 3.4:-79470792 | MS.gene77584:CDS |
| TTGCCAGTGGCTTCTGCTGG+GGG | 0.482095 | 3.4:-79470595 | MS.gene77584:CDS |
| ACTTTCCAGCTGCCATTACA+AGG | 0.493916 | 3.4:+79471007 | None:intergenic |
| TGAGATCGAAACAAATGAAT+CGG | 0.494220 | 3.4:-79469963 | MS.gene77584:CDS |
| TGAGCAAGCCTTTGTTCCCT+AGG | 0.496907 | 3.4:+79469995 | None:intergenic |
| AGCCTCGAAGAGATCGAAGC+AGG | 0.497044 | 3.4:-79470936 | MS.gene77584:CDS |
| TGTGGCCTTGTAATGGCAGC+TGG | 0.498116 | 3.4:-79471012 | MS.gene77584:CDS |
| GATGGTCAGATCATGCTTGT+GGG | 0.503093 | 3.4:-79470153 | MS.gene77584:CDS |
| ACAACATAGCAAGGAAAGAA+TGG | 0.504668 | 3.4:-79469930 | MS.gene77584:CDS |
| ACAATCAACTTTGTGGGCAG+TGG | 0.506047 | 3.4:+79470619 | None:intergenic |
| GACGGCATACAGAGCGGAAG+CGG | 0.516102 | 3.4:+79470797 | None:intergenic |
| ATCTCTTCGAGGCTTCTTAG+CGG | 0.517356 | 3.4:+79470945 | None:intergenic |
| TGAAGTTAAGGGACAATGGC+AGG | 0.518383 | 3.4:+79470324 | None:intergenic |
| GAGTCGTCAGGAAACATGCC+TGG | 0.522784 | 3.4:-79470356 | MS.gene77584:intron |
| TTTCAAAGAAGGGTCATACA+TGG | 0.524726 | 3.4:+79470666 | None:intergenic |
| CACGTCAGAATGACTTGCCT+AGG | 0.525727 | 3.4:-79470012 | MS.gene77584:CDS |
| TTTGCCAGTGGCTTCTGCTG+GGG | 0.528979 | 3.4:-79470596 | MS.gene77584:CDS |
| CAGCTCGGTCAGTTAAGGTC+TGG | 0.530478 | 3.4:-79470495 | MS.gene77584:CDS |
| CCTTCAAGGAGTTGCCAGCT+CGG | 0.530770 | 3.4:-79470510 | MS.gene77584:CDS |
| AATGCCAAATATATGGTGCT+TGG | 0.531763 | 3.4:-79470049 | MS.gene77584:CDS |
| AGTTTGCGACCTGAAGTTAA+GGG | 0.541286 | 3.4:+79470313 | None:intergenic |
| CAAGCCACCGCGGTCAAGCA+TGG | 0.544564 | 3.4:+79469835 | None:intergenic |
| GCCTCGAAGAGATCGAAGCA+GGG | 0.544882 | 3.4:-79470935 | MS.gene77584:CDS |
| AGAGCTATCTTCGAGGTCAA+AGG | 0.555308 | 3.4:+79471047 | None:intergenic |
| CCAGCAAGGAATTCCATTGA+CGG | 0.555971 | 3.4:+79470779 | None:intergenic |
| CATCTACAGAGCTATCTTCG+AGG | 0.556179 | 3.4:+79471040 | None:intergenic |
| TTAGCGGGTGGAGGCTTCAG+AGG | 0.556937 | 3.4:+79470961 | None:intergenic |
| AAGAATGGCTGAAGGTTCCT+GGG | 0.558151 | 3.4:-79469915 | MS.gene77584:CDS |
| CTTCGAGGCTTCTTAGCGGG+TGG | 0.561487 | 3.4:+79470949 | None:intergenic |
| TCAGTCAATGTAGTGGCGCT+GGG | 0.563553 | 3.4:+79470195 | None:intergenic |
| ATCGAAACAAATGAATCGGT+TGG | 0.564495 | 3.4:-79469959 | MS.gene77584:CDS |
| CCTTGAAGGATTGAGTTAGT+GGG | 0.570394 | 3.4:+79470524 | None:intergenic |
| CACACTCTAGCTGAGTATGA+TGG | 0.574409 | 3.4:-79470171 | MS.gene77584:CDS |
| TGACTGTTGTGGCCTTGTAA+TGG | 0.580674 | 3.4:-79471019 | MS.gene77584:CDS |
| TGGGTTATGGTGTAAAACCA+TGG | 0.586647 | 3.4:+79470709 | None:intergenic |
| GCTGTAGGGATGACAGTGAA+TGG | 0.589049 | 3.4:-79470463 | MS.gene77584:CDS |
| TGGTGCCGAATGGGAAGAAA+CGG | 0.591767 | 3.4:-79469888 | MS.gene77584:CDS |
| TGCAGAGAATGACACTCTTG+TGG | 0.591979 | 3.4:-79470086 | MS.gene77584:CDS |
| AAGTCCCGCAAGAAAATCCA+TGG | 0.594704 | 3.4:-79470726 | MS.gene77584:CDS |
| TCATCCATGCTTGACCGCGG+TGG | 0.597901 | 3.4:-79469839 | MS.gene77584:CDS |
| GCTGAAGGTTCCTGGGTGTG+TGG | 0.600893 | 3.4:-79469908 | MS.gene77584:CDS |
| TGGTTACTTACAACATAGCA+AGG | 0.602101 | 3.4:-79469939 | MS.gene77584:CDS |
| GAGAATGACACTCTTGTGGA+AGG | 0.607066 | 3.4:-79470082 | MS.gene77584:CDS |
| ATGACTTGCCTAGGGAACAA+AGG | 0.609468 | 3.4:-79470003 | MS.gene77584:CDS |
| AAAGCTTTGAGAAGCCAGCA+AGG | 0.612786 | 3.4:+79470765 | None:intergenic |
| TGATGGTCAGATCATGCTTG+TGG | 0.614839 | 3.4:-79470154 | MS.gene77584:CDS |
| CAGACCTTAACTGACCGAGC+TGG | 0.631524 | 3.4:+79470496 | None:intergenic |
| ACGTCAGAATGACTTGCCTA+GGG | 0.644663 | 3.4:-79470011 | MS.gene77584:CDS |
| AATGACACTCTTGTGGAAGG+AGG | 0.644741 | 3.4:-79470079 | MS.gene77584:CDS |
| AGCAAGGAAAGAATGGCTGA+AGG | 0.648548 | 3.4:-79469923 | MS.gene77584:CDS |
| CCATTCGGCACCACACACCC+AGG | 0.649604 | 3.4:+79469898 | None:intergenic |
| ATTTCATCCATGCTTGACCG+CGG | 0.649663 | 3.4:-79469842 | MS.gene77584:CDS |
| CGAATGGGAAGAAACGGCAG+TGG | 0.651054 | 3.4:-79469882 | MS.gene77584:CDS |
| GTGGGCAGTGGTGATATAGA+CGG | 0.653208 | 3.4:+79470631 | None:intergenic |
| AGCCACGTGTGTATGCATAT+GGG | 0.660040 | 3.4:-79470112 | MS.gene77584:CDS |
| GCTATCTTCGAGGTCAAAGG+AGG | 0.661871 | 3.4:+79471050 | None:intergenic |
| CCTCGAAGAGATCGAAGCAG+GGG | 0.663964 | 3.4:-79470934 | MS.gene77584:CDS |
| CTCCCATATGCATACACACG+TGG | 0.665284 | 3.4:+79470110 | None:intergenic |
| TACATACATAGAAGTTACGA+TGG | 0.667085 | 3.4:+79470548 | None:intergenic |
| AGCTCTGTAGATGACTGTTG+TGG | 0.670937 | 3.4:-79471030 | MS.gene77584:CDS |
| GACCTGAAGTTAAGGGACAA+TGG | 0.674409 | 3.4:+79470320 | None:intergenic |
| AGTGTGGTCAGTCAATGTAG+TGG | 0.674886 | 3.4:+79470188 | None:intergenic |
| TCCATTGACGGCATACAGAG+CGG | 0.675929 | 3.4:+79470791 | None:intergenic |
| CATCATACTCAGCTAGAGTG+TGG | 0.675947 | 3.4:+79470172 | None:intergenic |
| AAACGGCAGTGGATAGCATG+TGG | 0.678505 | 3.4:-79469871 | MS.gene77584:CDS |
| CAAGGAAAATCAAGCCACCG+CGG | 0.733913 | 3.4:+79469825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGGATAAACAAAAATATT+TGG | + | chr3.4:79469883-79469902 | None:intergenic | 15.0% |
| ! | AAGTTATGGAACAAGAAATA+TGG | - | chr3.4:79470082-79470101 | MS.gene77584:CDS | 25.0% |
| ! | AGAATATGAAGTTTATGACT+CGG | - | chr3.4:79470572-79470591 | MS.gene77584:CDS | 25.0% |
| ! | TAGAAAAACCAACATTTACA+GGG | + | chr3.4:79469841-79469860 | None:intergenic | 25.0% |
| !! | TTTTCCTGAAGATTTATTTG+AGG | - | chr3.4:79470110-79470129 | MS.gene77584:CDS | 25.0% |
| AATGGAAATTCAATCAGTTC+AGG | - | chr3.4:79470519-79470538 | MS.gene77584:CDS | 30.0% | |
| GTAGAAAAACCAACATTTAC+AGG | + | chr3.4:79469842-79469861 | None:intergenic | 30.0% | |
| TACATACATAGAAGTTACGA+TGG | + | chr3.4:79470419-79470438 | None:intergenic | 30.0% | |
| TAGACAGAATGCCAAATATA+TGG | - | chr3.4:79470908-79470927 | MS.gene77584:CDS | 30.0% | |
| TGAGATCGAAACAAATGAAT+CGG | - | chr3.4:79471001-79471020 | MS.gene77584:CDS | 30.0% | |
| ! | TAAAACCATGGATTTTCTTG+CGG | + | chr3.4:79470246-79470265 | None:intergenic | 30.0% |
| ! | TCCTGAATTCACATTTTCAT+GGG | + | chr3.4:79470277-79470296 | None:intergenic | 30.0% |
| ! | TTGTTTATCCATTTTGCAGA+TGG | - | chr3.4:79469890-79469909 | MS.gene77584:CDS | 30.0% |
| !! | ATTCACATTTTCATGGGTTA+TGG | + | chr3.4:79470271-79470290 | None:intergenic | 30.0% |
| !! | TTAGTTTGCTTTCTTGACAT+TGG | - | chr3.4:79470393-79470412 | MS.gene77584:intron | 30.0% |
| !! | TTGGTTTTTCTACTTGACAT+GGG | - | chr3.4:79469849-79469868 | MS.gene77584:CDS | 30.0% |
| AAGTCCAAGCACCATATATT+TGG | + | chr3.4:79470922-79470941 | None:intergenic | 35.0% | |
| AATGCCAAATATATGGTGCT+TGG | - | chr3.4:79470915-79470934 | MS.gene77584:CDS | 35.0% | |
| ACAACATAGCAAGGAAAGAA+TGG | - | chr3.4:79471034-79471053 | MS.gene77584:CDS | 35.0% | |
| ACAGCCTCAAATAAATCTTC+AGG | + | chr3.4:79470117-79470136 | None:intergenic | 35.0% | |
| ACCCATGAAAATGTGAATTC+AGG | - | chr3.4:79470273-79470292 | MS.gene77584:intron | 35.0% | |
| ATCCAGTACAAGTGAAGTTA+TGG | - | chr3.4:79470068-79470087 | MS.gene77584:CDS | 35.0% | |
| ATCGAAACAAATGAATCGGT+TGG | - | chr3.4:79471005-79471024 | MS.gene77584:CDS | 35.0% | |
| ATCTGAACGCATGAAGTATA+GGG | + | chr3.4:79470693-79470712 | None:intergenic | 35.0% | |
| GGCAAAACAATCAACTTTGT+GGG | + | chr3.4:79470354-79470373 | None:intergenic | 35.0% | |
| TGGCAAAACAATCAACTTTG+TGG | + | chr3.4:79470355-79470374 | None:intergenic | 35.0% | |
| TGGTTACTTACAACATAGCA+AGG | - | chr3.4:79471025-79471044 | MS.gene77584:CDS | 35.0% | |
| TTCCATAACTTCACTTGTAC+TGG | + | chr3.4:79470073-79470092 | None:intergenic | 35.0% | |
| TTTCAAAGAAGGGTCATACA+TGG | + | chr3.4:79470301-79470320 | None:intergenic | 35.0% | |
| TTTCCTATGATATGGCAACA+GGG | - | chr3.4:79470726-79470745 | MS.gene77584:CDS | 35.0% | |
| ! | AAAACCATGGATTTTCTTGC+GGG | + | chr3.4:79470245-79470264 | None:intergenic | 35.0% |
| ! | AAGCTTTTCTAAGCAATGTG+CGG | - | chr3.4:79470215-79470234 | MS.gene77584:CDS | 35.0% |
| ! | ATATGGTGCTTGGACTTTTA+TGG | - | chr3.4:79470925-79470944 | MS.gene77584:CDS | 35.0% |
| ! | ATGACCCTTCTTTGAAAAAG+TGG | - | chr3.4:79470304-79470323 | MS.gene77584:intron | 35.0% |
| ! | CTCCTGAATTCACATTTTCA+TGG | + | chr3.4:79470278-79470297 | None:intergenic | 35.0% |
| ! | CTGGAAAGTCTCATAGTTTT+AGG | - | chr3.4:79469971-79469990 | MS.gene77584:CDS | 35.0% |
| ! | TGGTTTTTCTACTTGACATG+GGG | - | chr3.4:79469850-79469869 | MS.gene77584:CDS | 35.0% |
| !! | AAAGTTGATTGTTTTGCCAG+TGG | - | chr3.4:79470356-79470375 | MS.gene77584:intron | 35.0% |
| !!! | GATGCCACTTTTTCAAAGAA+GGG | + | chr3.4:79470311-79470330 | None:intergenic | 35.0% |
| !!! | GTTGGTTTTTCTACTTGACA+TGG | - | chr3.4:79469848-79469867 | MS.gene77584:CDS | 35.0% |
| !!! | TGATGCCACTTTTTCAAAGA+AGG | + | chr3.4:79470312-79470331 | None:intergenic | 35.0% |
| AGTTTGCGACCTGAAGTTAA+GGG | + | chr3.4:79470654-79470673 | None:intergenic | 40.0% | |
| CCTTGAAGGATTGAGTTAGT+GGG | + | chr3.4:79470443-79470462 | None:intergenic | 40.0% | |
| GATCTGAACGCATGAAGTAT+AGG | + | chr3.4:79470694-79470713 | None:intergenic | 40.0% | |
| GTTTCCTATGATATGGCAAC+AGG | - | chr3.4:79470725-79470744 | MS.gene77584:CDS | 40.0% | |
| TCCTTGAAGGATTGAGTTAG+TGG | + | chr3.4:79470444-79470463 | None:intergenic | 40.0% | |
| TGGGTTATGGTGTAAAACCA+TGG | + | chr3.4:79470258-79470277 | None:intergenic | 40.0% | |
| TTCAGGCTACAAGATCCTAT+GGG | - | chr3.4:79470536-79470555 | MS.gene77584:CDS | 40.0% | |
| TTCCTATGATATGGCAACAG+GGG | - | chr3.4:79470727-79470746 | MS.gene77584:CDS | 40.0% | |
| ! | AGGGGTTGTTTCCTATGATA+TGG | - | chr3.4:79470718-79470737 | MS.gene77584:CDS | 40.0% |
| !! | ATGACTCGGTGAAAAACTCT+TGG | - | chr3.4:79470586-79470605 | MS.gene77584:CDS | 40.0% |
| AAAGAATGGCTGAAGGTTCC+TGG | - | chr3.4:79471048-79471067 | MS.gene77584:CDS | 45.0% | |
| AACCCCTGTTGCCATATCAT+AGG | + | chr3.4:79470732-79470751 | None:intergenic | 45.0% | |
| AAGAATGGCTGAAGGTTCCT+GGG | - | chr3.4:79471049-79471068 | MS.gene77584:CDS | 45.0% | |
| AAGGAGGTCCATCTGCAAAA+TGG | + | chr3.4:79469901-79469920 | None:intergenic | 45.0% | |
| AAGTCCCGCAAGAAAATCCA+TGG | - | chr3.4:79470238-79470257 | MS.gene77584:intron | 45.0% | |
| AATGACACTCTTGTGGAAGG+AGG | - | chr3.4:79470885-79470904 | MS.gene77584:CDS | 45.0% | |
| AATGGCAGGTTCATACTTCC+AGG | + | chr3.4:79470629-79470648 | None:intergenic | 45.0% | |
| ACAATCAACTTTGTGGGCAG+TGG | + | chr3.4:79470348-79470367 | None:intergenic | 45.0% | |
| ACTTTCCAGCTGCCATTACA+AGG | + | chr3.4:79469960-79469979 | None:intergenic | 45.0% | |
| AGAGCTATCTTCGAGGTCAA+AGG | + | chr3.4:79469920-79469939 | None:intergenic | 45.0% | |
| AGCAAGGAAAGAATGGCTGA+AGG | - | chr3.4:79471041-79471060 | MS.gene77584:CDS | 45.0% | |
| AGCCACGTGTGTATGCATAT+GGG | - | chr3.4:79470852-79470871 | MS.gene77584:CDS | 45.0% | |
| AGCTCTGTAGATGACTGTTG+TGG | - | chr3.4:79469934-79469953 | MS.gene77584:CDS | 45.0% | |
| AGTGTGGTCAGTCAATGTAG+TGG | + | chr3.4:79470779-79470798 | None:intergenic | 45.0% | |
| ATCTCTTCGAGGCTTCTTAG+CGG | + | chr3.4:79470022-79470041 | None:intergenic | 45.0% | |
| ATGATATGGCAACAGGGGTT+TGG | - | chr3.4:79470732-79470751 | MS.gene77584:CDS | 45.0% | |
| ATTTCATCCATGCTTGACCG+CGG | - | chr3.4:79471122-79471141 | MS.gene77584:intron | 45.0% | |
| CACACTCTAGCTGAGTATGA+TGG | - | chr3.4:79470793-79470812 | MS.gene77584:CDS | 45.0% | |
| CAGTTTGCGACCTGAAGTTA+AGG | + | chr3.4:79470655-79470674 | None:intergenic | 45.0% | |
| CATCATACTCAGCTAGAGTG+TGG | + | chr3.4:79470795-79470814 | None:intergenic | 45.0% | |
| CATCTACAGAGCTATCTTCG+AGG | + | chr3.4:79469927-79469946 | None:intergenic | 45.0% | |
| CCAGCAAGGAATTCCATTGA+CGG | + | chr3.4:79470188-79470207 | None:intergenic | 45.0% | |
| CCCACTAACTCAATCCTTCA+AGG | - | chr3.4:79470440-79470459 | MS.gene77584:intron | 45.0% | |
| GAAAAACTCTTGGAGTCGTC+AGG | - | chr3.4:79470596-79470615 | MS.gene77584:CDS | 45.0% | |
| GACCTGAAGTTAAGGGACAA+TGG | + | chr3.4:79470647-79470666 | None:intergenic | 45.0% | |
| GAGAATGACACTCTTGTGGA+AGG | - | chr3.4:79470882-79470901 | MS.gene77584:CDS | 45.0% | |
| GATGGTCAGATCATGCTTGT+GGG | - | chr3.4:79470811-79470830 | MS.gene77584:CDS | 45.0% | |
| GTTCAGGCTACAAGATCCTA+TGG | - | chr3.4:79470535-79470554 | MS.gene77584:CDS | 45.0% | |
| TCATAGGAAACAACCCCTTC+TGG | + | chr3.4:79470716-79470735 | None:intergenic | 45.0% | |
| TCATGCGTTCAGATCCAGAA+GGG | - | chr3.4:79470699-79470718 | MS.gene77584:CDS | 45.0% | |
| TGAAGTTAAGGGACAATGGC+AGG | + | chr3.4:79470643-79470662 | None:intergenic | 45.0% | |
| TGATGGTCAGATCATGCTTG+TGG | - | chr3.4:79470810-79470829 | MS.gene77584:CDS | 45.0% | |
| TGCAGAGAATGACACTCTTG+TGG | - | chr3.4:79470878-79470897 | MS.gene77584:CDS | 45.0% | |
| TGCCATTGTCCCTTAACTTC+AGG | - | chr3.4:79470642-79470661 | MS.gene77584:CDS | 45.0% | |
| TTCATGCGTTCAGATCCAGA+AGG | - | chr3.4:79470698-79470717 | MS.gene77584:CDS | 45.0% | |
| ! | ACGTCAGAATGACTTGCCTA+GGG | - | chr3.4:79470953-79470972 | MS.gene77584:CDS | 45.0% |
| ! | ATGACTTGCCTAGGGAACAA+AGG | - | chr3.4:79470961-79470980 | MS.gene77584:CDS | 45.0% |
| !! | AAAGCTTTGAGAAGCCAGCA+AGG | + | chr3.4:79470202-79470221 | None:intergenic | 45.0% |
| !! | TGACTGTTGTGGCCTTGTAA+TGG | - | chr3.4:79469945-79469964 | MS.gene77584:CDS | 45.0% |
| AAACGGCAGTGGATAGCATG+TGG | - | chr3.4:79471093-79471112 | MS.gene77584:intron | 50.0% | |
| AAGCGGAAAAACGCGGCAAT+GGG | + | chr3.4:79470153-79470172 | None:intergenic | 50.0% | |
| AGGCTACAAGATCCTATGGG+TGG | - | chr3.4:79470539-79470558 | MS.gene77584:CDS | 50.0% | |
| CAGCCACGTGTGTATGCATA+TGG | - | chr3.4:79470851-79470870 | MS.gene77584:CDS | 50.0% | |
| CATGCGTTCAGATCCAGAAG+GGG | - | chr3.4:79470700-79470719 | MS.gene77584:CDS | 50.0% | |
| CCGTCAATGGAATTCCTTGC+TGG | - | chr3.4:79470185-79470204 | MS.gene77584:CDS | 50.0% | |
| CTCCCATATGCATACACACG+TGG | + | chr3.4:79470857-79470876 | None:intergenic | 50.0% | |
| GCTATCTTCGAGGTCAAAGG+AGG | + | chr3.4:79469917-79469936 | None:intergenic | 50.0% | |
| GCTGTAGGGATGACAGTGAA+TGG | - | chr3.4:79470501-79470520 | MS.gene77584:CDS | 50.0% | |
| GGCTACAAGATCCTATGGGT+GGG | - | chr3.4:79470540-79470559 | MS.gene77584:CDS | 50.0% | |
| GTGGGCAGTGGTGATATAGA+CGG | + | chr3.4:79470336-79470355 | None:intergenic | 50.0% | |
| TCAGTCAATGTAGTGGCGCT+GGG | + | chr3.4:79470772-79470791 | None:intergenic | 50.0% | |
| TCCATTGACGGCATACAGAG+CGG | + | chr3.4:79470176-79470195 | None:intergenic | 50.0% | |
| TCTCTTCGAGGCTTCTTAGC+GGG | + | chr3.4:79470021-79470040 | None:intergenic | 50.0% | |
| TGAGCAAGCCTTTGTTCCCT+AGG | + | chr3.4:79470972-79470991 | None:intergenic | 50.0% | |
| ! | CACGTCAGAATGACTTGCCT+AGG | - | chr3.4:79470952-79470971 | MS.gene77584:CDS | 50.0% |
| ! | CACTGCCGTTTCTTCCCATT+CGG | + | chr3.4:79471084-79471103 | None:intergenic | 50.0% |
| ! | TGGTGCCGAATGGGAAGAAA+CGG | - | chr3.4:79471076-79471095 | MS.gene77584:intron | 50.0% |
| ! | TTTTGCCAGTGGCTTCTGCT+GGG | - | chr3.4:79470367-79470386 | MS.gene77584:intron | 50.0% |
| !! | ATCCTATGGGTGGGTTGTGA+TGG | - | chr3.4:79470549-79470568 | MS.gene77584:CDS | 50.0% |
| AGAGCGGAAGCGGAAAAACG+CGG | + | chr3.4:79470160-79470179 | None:intergenic | 55.0% | |
| AGCCTCGAAGAGATCGAAGC+AGG | - | chr3.4:79470028-79470047 | MS.gene77584:CDS | 55.0% | |
| CAGACCTTAACTGACCGAGC+TGG | + | chr3.4:79470471-79470490 | None:intergenic | 55.0% | |
| CAGCTCGGTCAGTTAAGGTC+TGG | - | chr3.4:79470469-79470488 | MS.gene77584:CDS | 55.0% | |
| CCTCGAAGAGATCGAAGCAG+GGG | - | chr3.4:79470030-79470049 | MS.gene77584:CDS | 55.0% | |
| CCTTCAAGGAGTTGCCAGCT+CGG | - | chr3.4:79470454-79470473 | MS.gene77584:CDS | 55.0% | |
| CGAAGCAGGGGCAAATCATC+AGG | - | chr3.4:79470042-79470061 | MS.gene77584:CDS | 55.0% | |
| CGAATGGGAAGAAACGGCAG+TGG | - | chr3.4:79471082-79471101 | MS.gene77584:intron | 55.0% | |
| CTCCATCACAACCCACCCAT+AGG | + | chr3.4:79470554-79470573 | None:intergenic | 55.0% | |
| GAAGCGGAAAAACGCGGCAA+TGG | + | chr3.4:79470154-79470173 | None:intergenic | 55.0% | |
| GAGTCGTCAGGAAACATGCC+TGG | - | chr3.4:79470608-79470627 | MS.gene77584:CDS | 55.0% | |
| GCCTCGAAGAGATCGAAGCA+GGG | - | chr3.4:79470029-79470048 | MS.gene77584:CDS | 55.0% | |
| GTCAGTCAATGTAGTGGCGC+TGG | + | chr3.4:79470773-79470792 | None:intergenic | 55.0% | |
| GTTGCCAGCTCGGTCAGTTA+AGG | - | chr3.4:79470464-79470483 | MS.gene77584:CDS | 55.0% | |
| TCCGCTCTGTATGCCGTCAA+TGG | - | chr3.4:79470172-79470191 | MS.gene77584:CDS | 55.0% | |
| TCTGGTCTCGCGTTGCTGTA+GGG | - | chr3.4:79470487-79470506 | MS.gene77584:CDS | 55.0% | |
| TTTGCCAGTGGCTTCTGCTG+GGG | - | chr3.4:79470368-79470387 | MS.gene77584:intron | 55.0% | |
| ! | GTTTTGCCAGTGGCTTCTGC+TGG | - | chr3.4:79470366-79470385 | MS.gene77584:intron | 55.0% |
| !! | TGTGGCCTTGTAATGGCAGC+TGG | - | chr3.4:79469952-79469971 | MS.gene77584:CDS | 55.0% |
| AAACCCCCAGCAGAAGCCAC+TGG | + | chr3.4:79470375-79470394 | None:intergenic | 60.0% | |
| CCCCTGCTTCGATCTCTTCG+AGG | + | chr3.4:79470033-79470052 | None:intergenic | 60.0% | |
| CCGAGCTGGCAACTCCTTGA+AGG | + | chr3.4:79470457-79470476 | None:intergenic | 60.0% | |
| CTTCGAGGCTTCTTAGCGGG+TGG | + | chr3.4:79470018-79470037 | None:intergenic | 60.0% | |
| GACGGCATACAGAGCGGAAG+CGG | + | chr3.4:79470170-79470189 | None:intergenic | 60.0% | |
| GCTGAAGGTTCCTGGGTGTG+TGG | - | chr3.4:79471056-79471075 | MS.gene77584:CDS | 60.0% | |
| GTCTGGTCTCGCGTTGCTGT+AGG | - | chr3.4:79470486-79470505 | MS.gene77584:CDS | 60.0% | |
| TCATCCATGCTTGACCGCGG+TGG | - | chr3.4:79471125-79471144 | MS.gene77584:intron | 60.0% | |
| TTGCCAGTGGCTTCTGCTGG+GGG | - | chr3.4:79470369-79470388 | MS.gene77584:intron | 60.0% | |
| ! | CTGGGTGTGTGGTGCCGAAT+GGG | - | chr3.4:79471067-79471086 | MS.gene77584:intron | 60.0% |
| ! | TTAGCGGGTGGAGGCTTCAG+AGG | + | chr3.4:79470006-79470025 | None:intergenic | 60.0% |
| CAAGCCACCGCGGTCAAGCA+TGG | + | chr3.4:79471132-79471151 | None:intergenic | 65.0% | |
| CCATTCGGCACCACACACCC+AGG | + | chr3.4:79471069-79471088 | None:intergenic | 65.0% | |
| ! | CCTGGGTGTGTGGTGCCGAA+TGG | - | chr3.4:79471066-79471085 | MS.gene77584:intron | 65.0% |
| ! | CGAGGCTTCTTAGCGGGTGG+AGG | + | chr3.4:79470015-79470034 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 79469834 | 79471152 | 79469834 | ID=MS.gene77584 |
| chr3.4 | mRNA | 79469834 | 79471152 | 79469834 | ID=MS.gene77584.t1;Parent=MS.gene77584 |
| chr3.4 | exon | 79471145 | 79471152 | 79471145 | ID=MS.gene77584.t1.exon1;Parent=MS.gene77584.t1 |
| chr3.4 | CDS | 79471145 | 79471152 | 79471145 | ID=cds.MS.gene77584.t1;Parent=MS.gene77584.t1 |
| chr3.4 | exon | 79470452 | 79471077 | 79470452 | ID=MS.gene77584.t1.exon2;Parent=MS.gene77584.t1 |
| chr3.4 | CDS | 79470452 | 79471077 | 79470452 | ID=cds.MS.gene77584.t1;Parent=MS.gene77584.t1 |
| chr3.4 | exon | 79470323 | 79470368 | 79470323 | ID=MS.gene77584.t1.exon3;Parent=MS.gene77584.t1 |
| chr3.4 | CDS | 79470323 | 79470368 | 79470323 | ID=cds.MS.gene77584.t1;Parent=MS.gene77584.t1 |
| chr3.4 | exon | 79469834 | 79470239 | 79469834 | ID=MS.gene77584.t1.exon4;Parent=MS.gene77584.t1 |
| chr3.4 | CDS | 79469834 | 79470239 | 79469834 | ID=cds.MS.gene77584.t1;Parent=MS.gene77584.t1 |
| Gene Sequence |
| Protein sequence |
