AlfalfaGEDB | MS.gene59876

M. sativa cultivar XinJiangDaYe / MS.gene59876

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016603 GO:0016740 GO:0016746 GO:0016755 GO:0140096
PFAM: PF12049.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59876.t1	XP_003628241.1	88.1	118	5	1	15	123	201	318	2.10E-49	204.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59876.t1	G7L8J3	88.1	118	5	1	15	123	201	318	1.5e-49	204.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene59876	0.829188	5.92E-55	-1.69E-46
MS.gene049378	MS.gene59876	0.817933	2.55E-52	-1.69E-46
MS.gene049382	MS.gene59876	0.803324	3.68E-49	-1.69E-46
MS.gene049756	MS.gene59876	0.82292	1.83E-53	-1.69E-46
MS.gene049758	MS.gene59876	0.836315	1.01E-56	-1.69E-46
MS.gene04984	MS.gene59876	0.81415	1.78E-51	-1.69E-46
MS.gene049915	MS.gene59876	0.84556	3.76E-59	-1.69E-46
MS.gene050226	MS.gene59876	0.839813	1.27E-57	-1.69E-46
MS.gene050227	MS.gene59876	0.849025	4.21E-60	-1.69E-46
MS.gene050228	MS.gene59876	0.84947	3.17E-60	-1.69E-46
MS.gene050277	MS.gene59876	0.81544	9.23E-52	-1.69E-46
MS.gene050303	MS.gene59876	0.833664	4.68E-56	-1.69E-46
MS.gene050319	MS.gene59876	-0.805883	1.08E-49	-1.69E-46
MS.gene050330	MS.gene59876	-0.831153	1.96E-55	-1.69E-46
MS.gene050438	MS.gene59876	0.833508	5.12E-56	-1.69E-46
MS.gene050442	MS.gene59876	0.809458	1.87E-50	-1.69E-46
MS.gene050558	MS.gene59876	0.814452	1.53E-51	-1.69E-46
MS.gene050623	MS.gene59876	-0.833386	5.49E-56	-1.69E-46
MS.gene050642	MS.gene59876	0.832966	6.99E-56	-1.69E-46
MS.gene050719	MS.gene59876	0.854789	9.74E-62	-1.69E-46
MS.gene050721	MS.gene59876	0.857946	1.15E-62	-1.69E-46
MS.gene050724	MS.gene59876	0.84735	1.22E-59	-1.69E-46
MS.gene050815	MS.gene59876	0.818345	2.06E-52	-1.69E-46
MS.gene050824	MS.gene59876	0.817642	2.97E-52	-1.69E-46
MS.gene051245	MS.gene59876	0.811142	8.12E-51	-1.69E-46
MS.gene051349	MS.gene59876	0.825689	4.09E-54	-1.69E-46
MS.gene051626	MS.gene59876	0.818552	1.85E-52	-1.69E-46
MS.gene051633	MS.gene59876	0.817509	3.18E-52	-1.69E-46
MS.gene051662	MS.gene59876	0.820786	5.70E-53	-1.69E-46
MS.gene051663	MS.gene59876	0.810949	8.94E-51	-1.69E-46
MS.gene051757	MS.gene59876	0.807193	5.70E-50	-1.69E-46
MS.gene051860	MS.gene59876	0.812123	4.97E-51	-1.69E-46
MS.gene051983	MS.gene59876	0.814341	1.62E-51	-1.69E-46
MS.gene052048	MS.gene59876	-0.800087	1.70E-48	-1.69E-46
MS.gene052176	MS.gene59876	0.842231	2.93E-58	-1.69E-46
MS.gene052256	MS.gene59876	0.805639	1.21E-49	-1.69E-46
MS.gene052286	MS.gene59876	0.820559	6.43E-53	-1.69E-46
MS.gene052302	MS.gene59876	0.85707	2.10E-62	-1.69E-46
MS.gene052309	MS.gene59876	-0.807548	4.79E-50	-1.69E-46
MS.gene052376	MS.gene59876	0.873564	1.33E-67	-1.69E-46
MS.gene052600	MS.gene59876	0.816095	6.60E-52	-1.69E-46
MS.gene052613	MS.gene59876	0.806603	7.59E-50	-1.69E-46
MS.gene052856	MS.gene59876	0.853064	3.06E-61	-1.69E-46
MS.gene052858	MS.gene59876	0.826203	3.09E-54	-1.69E-46
MS.gene052860	MS.gene59876	0.821908	3.14E-53	-1.69E-46
MS.gene053074	MS.gene59876	0.818651	1.75E-52	-1.69E-46
MS.gene053132	MS.gene59876	0.808589	2.88E-50	-1.69E-46
MS.gene053184	MS.gene59876	0.801997	6.91E-49	-1.69E-46
MS.gene053437	MS.gene59876	0.811709	6.11E-51	-1.69E-46
MS.gene053630	MS.gene59876	0.818291	2.12E-52	-1.69E-46
MS.gene053836	MS.gene59876	0.844141	9.09E-59	-1.69E-46
MS.gene054184	MS.gene59876	0.834424	3.02E-56	-1.69E-46
MS.gene054185	MS.gene59876	0.842851	2.01E-58	-1.69E-46
MS.gene054472	MS.gene59876	0.809683	1.68E-50	-1.69E-46
MS.gene054473	MS.gene59876	0.825375	4.85E-54	-1.69E-46
MS.gene054542	MS.gene59876	0.818319	2.09E-52	-1.69E-46
MS.gene054667	MS.gene59876	0.814347	1.61E-51	-1.69E-46
MS.gene054930	MS.gene59876	0.824893	6.31E-54	-1.69E-46
MS.gene054942	MS.gene59876	0.835478	1.64E-56	-1.69E-46
MS.gene054983	MS.gene59876	0.80807	3.71E-50	-1.69E-46
MS.gene055133	MS.gene59876	0.831848	1.32E-55	-1.69E-46
MS.gene056069	MS.gene59876	-0.836457	9.26E-57	-1.69E-46
MS.gene056432	MS.gene59876	0.810201	1.30E-50	-1.69E-46
MS.gene056552	MS.gene59876	0.813963	1.96E-51	-1.69E-46
MS.gene056631	MS.gene59876	0.818421	1.98E-52	-1.69E-46
MS.gene056760	MS.gene59876	0.802076	6.66E-49	-1.69E-46
MS.gene056875	MS.gene59876	0.832924	7.16E-56	-1.69E-46
MS.gene056979	MS.gene59876	0.811231	7.76E-51	-1.69E-46
MS.gene057057	MS.gene59876	0.802346	5.86E-49	-1.69E-46
MS.gene057121	MS.gene59876	0.804511	2.09E-49	-1.69E-46
MS.gene057140	MS.gene59876	0.827927	1.19E-54	-1.69E-46
MS.gene057260	MS.gene59876	0.832385	9.74E-56	-1.69E-46
MS.gene057439	MS.gene59876	0.808958	2.40E-50	-1.69E-46
MS.gene057754	MS.gene59876	0.830093	3.57E-55	-1.69E-46
MS.gene057967	MS.gene59876	0.802125	6.51E-49	-1.69E-46
MS.gene057980	MS.gene59876	0.808933	2.43E-50	-1.69E-46
MS.gene058084	MS.gene59876	0.836837	7.41E-57	-1.69E-46
MS.gene058168	MS.gene59876	0.823977	1.04E-53	-1.69E-46
MS.gene058182	MS.gene59876	0.854531	1.16E-61	-1.69E-46
MS.gene058399	MS.gene59876	0.823276	1.51E-53	-1.69E-46
MS.gene058749	MS.gene59876	0.832398	9.67E-56	-1.69E-46
MS.gene058851	MS.gene59876	0.818246	2.17E-52	-1.69E-46
MS.gene058906	MS.gene59876	0.867377	1.43E-65	-1.69E-46
MS.gene058982	MS.gene59876	0.809966	1.46E-50	-1.69E-46
MS.gene059124	MS.gene59876	0.817576	3.07E-52	-1.69E-46
MS.gene059249	MS.gene59876	0.843206	1.62E-58	-1.69E-46
MS.gene059421	MS.gene59876	0.800973	1.12E-48	-1.69E-46
MS.gene059569	MS.gene59876	0.8045	2.10E-49	-1.69E-46
MS.gene05984	MS.gene59876	0.807951	3.93E-50	-1.69E-46
MS.gene06001	MS.gene59876	0.823214	1.56E-53	-1.69E-46
MS.gene060165	MS.gene59876	0.823246	1.54E-53	-1.69E-46
MS.gene060297	MS.gene59876	0.80476	1.85E-49	-1.69E-46
MS.gene060404	MS.gene59876	0.816022	6.85E-52	-1.69E-46
MS.gene060476	MS.gene59876	0.841953	3.48E-58	-1.69E-46
MS.gene060586	MS.gene59876	0.806325	8.69E-50	-1.69E-46
MS.gene060619	MS.gene59876	0.830449	2.92E-55	-1.69E-46
MS.gene060998	MS.gene59876	0.805529	1.28E-49	-1.69E-46
MS.gene061040	MS.gene59876	-0.803508	3.37E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59876.t1	MTR_8g046180	88.136	118	5	1	15	123	201	318	1.42e-68	209
MS.gene59876.t1	MTR_8g046180	81.395	86	7	1	15	91	201	286	6.22e-42	140
MS.gene59876.t1	MTR_6g004030	38.462	117	63	1	16	123	185	301	6.59e-25	96.3
MS.gene59876.t1	MTR_8g446720	74.000	50	12	1	74	123	8	56	2.00e-18	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59876.t1	AT4G29400	68.376	117	28	1	16	123	186	302	2.15e-52	167
MS.gene59876.t1	AT5G08400	41.026	117	60	1	16	123	184	300	6.60e-26	99.0
MS.gene59876.t1	AT5G08400	41.026	117	60	1	16	123	217	333	7.04e-26	99.4

Find 25 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTGAATTTGGATCTTTGC+AGG	0.257367	8.4:+55085199	MS.gene59876:CDS
ACAACTTACTTGCATGTTCA+TGG	0.309285	8.4:-55085267	None:intergenic
CCAACTACATTTACAACATT+GGG	0.309887	8.4:+55085467	MS.gene59876:CDS
ACCAAGTTACGATTATATTC+AGG	0.316714	8.4:+55085430	MS.gene59876:CDS
GATTTAACATCAGAGGATTC+AGG	0.317872	8.4:+55085883	MS.gene59876:CDS
ACATCAGAGGATTCAGGTTA+TGG	0.330697	8.4:+55085889	MS.gene59876:CDS
GTGGAGAAGAATTTGAGAAT+TGG	0.339234	8.4:+55085854	MS.gene59876:CDS
TGATATAACAGGATAGATAT+TGG	0.346310	8.4:+55085620	MS.gene59876:intron
GATGCACACATGCTTCAATT+AGG	0.396292	8.4:+55085170	None:intergenic
ATCAAAACATCTAGAATCAA+TGG	0.409600	8.4:-55085653	None:intergenic
ACCAACTACATTTACAACAT+TGG	0.429357	8.4:+55085466	MS.gene59876:CDS
AGAATTTGAGAATTGGAAAG+AGG	0.431631	8.4:+55085861	MS.gene59876:CDS
CCCTGAATATAATCGTAACT+TGG	0.440268	8.4:-55085431	None:intergenic
GATATAACAGGATAGATATT+GGG	0.451457	8.4:+55085621	MS.gene59876:intron
CCAAGTTACGATTATATTCA+GGG	0.463616	8.4:+55085431	MS.gene59876:CDS
ATTCGAGCATGGCACATTGT+AGG	0.492114	8.4:+55085224	MS.gene59876:CDS
CCCAATGTTGTAAATGTAGT+TGG	0.509641	8.4:-55085467	None:intergenic
GGGATCAAGCAAGTAGTGTT+TGG	0.510754	8.4:+55085832	MS.gene59876:CDS
TTACAACATTGGGGATCTTG+AGG	0.542189	8.4:+55085477	MS.gene59876:CDS
ATCAAGCAAGTAGTGTTTGG+TGG	0.565132	8.4:+55085835	MS.gene59876:CDS
GAAAGAGGATTTAACATCAG+AGG	0.565258	8.4:+55085876	MS.gene59876:CDS
CTTTGCAGGCTATTCGAGCA+TGG	0.569213	8.4:+55085213	MS.gene59876:CDS
TGGCACATTGTAGGACGACT+TGG	0.571804	8.4:+55085233	MS.gene59876:CDS
CACATTGTAGGACGACTTGG+AGG	0.594434	8.4:+55085236	MS.gene59876:CDS
CAACTACATTTACAACATTG+GGG	0.624149	8.4:+55085468	MS.gene59876:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTCACTATAAATAGAA+AGG	-	chr8.4:55085339-55085358	None:intergenic	15.0%
!!!	ATTTATTTTTGTGATATAAC+AGG	+	chr8.4:55085609-55085628	MS.gene59876:intron	15.0%
!!	AAAAAACCAGATCAATTAAT+TGG	-	chr8.4:55085793-55085812	None:intergenic	20.0%
!!	AATTATATTGCTTATAGAAC+TGG	+	chr8.4:55085367-55085386	MS.gene59876:intron	20.0%
!!	ATTAATCCAATTAATTGATC+TGG	+	chr8.4:55085784-55085803	MS.gene59876:intron	20.0%
!	AATGAGAGAGAAAGAAAATT+TGG	-	chr8.4:55085313-55085332	None:intergenic	25.0%
!	GATATAACAGGATAGATATT+GGG	+	chr8.4:55085621-55085640	MS.gene59876:intron	25.0%
!	GTAGTTAATTAAACAAACAG+AGG	-	chr8.4:55085721-55085740	None:intergenic	25.0%
!	TGATATAACAGGATAGATAT+TGG	+	chr8.4:55085620-55085639	MS.gene59876:intron	25.0%
!!	ATCAAAACATCTAGAATCAA+TGG	-	chr8.4:55085656-55085675	None:intergenic	25.0%
!!	TAACAAAGCATTAAGAATGA+AGG	-	chr8.4:55085549-55085568	None:intergenic	25.0%
!!!	TGTTTTTTGATAGCTTTGTT+GGG	+	chr8.4:55085812-55085831	MS.gene59876:intron	25.0%
!!!	TTGTTTTTTGATAGCTTTGT+TGG	+	chr8.4:55085811-55085830	MS.gene59876:intron	25.0%
	AAACATGTAATGAGAGAGAA+AGG	-	chr8.4:55085762-55085781	None:intergenic	30.0%
	AACATGTAATGAGAGAGAAA+GGG	-	chr8.4:55085761-55085780	None:intergenic	30.0%
	AATTAAACAAACAGAGGAAG+TGG	-	chr8.4:55085715-55085734	None:intergenic	30.0%
	ACCAACTACATTTACAACAT+TGG	+	chr8.4:55085466-55085485	MS.gene59876:CDS	30.0%
	ACCAAGTTACGATTATATTC+AGG	+	chr8.4:55085430-55085449	MS.gene59876:CDS	30.0%
	AGAATTTGAGAATTGGAAAG+AGG	+	chr8.4:55085861-55085880	MS.gene59876:CDS	30.0%
	CAACTACATTTACAACATTG+GGG	+	chr8.4:55085468-55085487	MS.gene59876:CDS	30.0%
	CCAACTACATTTACAACATT+GGG	+	chr8.4:55085467-55085486	MS.gene59876:CDS	30.0%
	CCAAGTTACGATTATATTCA+GGG	+	chr8.4:55085431-55085450	MS.gene59876:CDS	30.0%
!	GAAAGAAAATTTGGTTTCCA+TGG	-	chr8.4:55085304-55085323	None:intergenic	30.0%
!	TTAGGAAGTTTTGCTGAATT+TGG	+	chr8.4:55085188-55085207	MS.gene59876:CDS	30.0%
	ACAACTTACTTGCATGTTCA+TGG	-	chr8.4:55085270-55085289	None:intergenic	35.0%
	CCCAATGTTGTAAATGTAGT+TGG	-	chr8.4:55085470-55085489	None:intergenic	35.0%
	CCCTGAATATAATCGTAACT+TGG	-	chr8.4:55085434-55085453	None:intergenic	35.0%
	GAAAGAGGATTTAACATCAG+AGG	+	chr8.4:55085876-55085895	MS.gene59876:CDS	35.0%
	GATTTAACATCAGAGGATTC+AGG	+	chr8.4:55085883-55085902	MS.gene59876:CDS	35.0%
	GTGGAGAAGAATTTGAGAAT+TGG	+	chr8.4:55085854-55085873	MS.gene59876:CDS	35.0%
	ATCAAGCAAGTAGTGTTTGG+TGG	+	chr8.4:55085835-55085854	MS.gene59876:CDS	40.0%
	TCTTGAGGTTCAAGACAACT+TGG	+	chr8.4:55085492-55085511	MS.gene59876:intron	40.0%
	TGCTGAATTTGGATCTTTGC+AGG	+	chr8.4:55085199-55085218	MS.gene59876:CDS	40.0%
	TTACAACATTGGGGATCTTG+AGG	+	chr8.4:55085477-55085496	MS.gene59876:CDS	40.0%
!	AAGACAACTTGGCTCGAATA+TGG	+	chr8.4:55085503-55085522	MS.gene59876:intron	40.0%
!	ACATCAGAGGATTCAGGTTA+TGG	+	chr8.4:55085889-55085908	MS.gene59876:CDS	40.0%
	AGTTGTTGTCTCTTCTGCCA+TGG	+	chr8.4:55085284-55085303	MS.gene59876:intron	45.0%
	ATTCGAGCATGGCACATTGT+AGG	+	chr8.4:55085224-55085243	MS.gene59876:CDS	45.0%
	GGGATCAAGCAAGTAGTGTT+TGG	+	chr8.4:55085832-55085851	MS.gene59876:CDS	45.0%
	CTTTGCAGGCTATTCGAGCA+TGG	+	chr8.4:55085213-55085232	MS.gene59876:CDS	50.0%
	GAGGAAGTGGAGATCGTACT+CGG	-	chr8.4:55085702-55085721	None:intergenic	50.0%
	TGGCACATTGTAGGACGACT+TGG	+	chr8.4:55085233-55085252	MS.gene59876:CDS	50.0%
!	CACATTGTAGGACGACTTGG+AGG	+	chr8.4:55085236-55085255	MS.gene59876:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	55085179	55085927	55085179	ID=MS.gene59876
chr8.4	mRNA	55085179	55085927	55085179	ID=MS.gene59876.t1;Parent=MS.gene59876
chr8.4	exon	55085179	55085280	55085179	ID=MS.gene59876.t1.exon1;Parent=MS.gene59876.t1
chr8.4	CDS	55085179	55085280	55085179	ID=cds.MS.gene59876.t1;Parent=MS.gene59876.t1
chr8.4	exon	55085404	55085498	55085404	ID=MS.gene59876.t1.exon2;Parent=MS.gene59876.t1
chr8.4	CDS	55085404	55085498	55085404	ID=cds.MS.gene59876.t1;Parent=MS.gene59876.t1
chr8.4	exon	55085631	55085702	55085631	ID=MS.gene59876.t1.exon3;Parent=MS.gene59876.t1
chr8.4	CDS	55085631	55085702	55085631	ID=cds.MS.gene59876.t1;Parent=MS.gene59876.t1
chr8.4	exon	55085825	55085927	55085825	ID=MS.gene59876.t1.exon4;Parent=MS.gene59876.t1
chr8.4	CDS	55085825	55085927	55085825	ID=cds.MS.gene59876.t1;Parent=MS.gene59876.t1

Gene Sequence

>MS.gene59876

ATGCTTCAATTAGGAAGTTTTGCTGAATTTGGATCTTTGCAGGCTATTCGAGCATGGCACATTGTAGGACGACTTGGAGGATGCAATGCCATGAACATGCAAGTAAGTTGTTGTCTCTTCTGCCATGGAAACCAAATTTTCTTTCTCTCTCATTTTTCCTTTCTATTTATAGTGAATTTTTAAAACATAATTATATTGCTTATAGAACTGGCATAATTTCTGCAGCTGTCACAGTCTGAGATAGAAAAACGACCAAGTTACGATTATATTCAGGGAGCGAATGTAACACCAACTACATTTACAACATTGGGGATCTTGAGGTTCAAGACAACTTGGCTCGAATATGGTGCAGAACTAATCGCATATTCCTTCATTCTTAATGCTTTGTTATGCATATTAGTTAATGTAATCTGAAGTGAAAATTCTAATAATTTATTTTTGTGATATAACAGGATAGATATTGGGACTAATGAACCATTGATTCTAGATGTTTTGATAAATGCATTGACACAGATAAGTTCCGAGTACGATCTCCACTTCCTCTGTTTGTTTAATTAACTACTGCAGTTCATTTATTTTCCCTTTCTCTCTCATTACATGTTTGTATTAATCCAATTAATTGATCTGGTTTTTTGTTTTTTGATAGCTTTGTTGGGATCAAGCAAGTAGTGTTTGGTGGAGAAGAATTTGAGAATTGGAAAGAGGATTTAACATCAGAGGATTCAGGTTATGGTGTTCATAAGATCTAG

Protein sequence

>MS.gene59876.t1

MLQLGSFAEFGSLQAIRAWHIVGRLGGCNAMNMQLSQSEIEKRPSYDYIQGANVTPTTFTTLGILRIDIGTNEPLILDVLINALTQISSDFVGIKQVVFGGEEFENWKEDLTSEDSGYGVHKI