AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07082


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07082.t1 MTR_7g109850 95.872 218 7 1 1 218 1 216 8.48e-152 420
MS.gene07082.t1 MTR_8g038785 87.313 134 15 1 1 134 1 132 1.12e-74 236
MS.gene07082.t1 MTR_2g005900 42.308 156 84 3 66 216 68 222 1.06e-29 110
MS.gene07082.t1 MTR_2g036690 37.267 161 99 2 51 210 71 230 7.38e-24 95.5
MS.gene07082.t1 MTR_2g036690 44.248 113 59 2 51 160 71 182 7.57e-20 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07082.t1 AT4G01940 67.822 202 55 3 25 218 32 231 4.89e-89 262
MS.gene07082.t1 AT5G49940 35.897 195 109 5 27 216 51 234 3.88e-26 101
MS.gene07082.t1 AT4G25910 33.180 217 128 6 12 212 11 226 9.38e-24 95.1
MS.gene07082.t1 AT5G49940 53.165 79 35 1 71 149 85 161 1.71e-21 87.8

Find 44 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACCACAACTATGAAAA+TGG 0.150095 7.2:+6191222 MS.gene07082:CDS
CACTTGCACGATATCTTTAA+TGG 0.162531 7.2:-6191281 None:intergenic
CGTGTTCTTAAGGAGAAATT+TGG 0.227866 7.2:+6191253 MS.gene07082:CDS
TTCGTTGGTATTGAACAAAT+TGG 0.356289 7.2:-6190887 None:intergenic
ATCCCGGAGAAGACTGGTTC+TGG 0.389633 7.2:-6191036 None:intergenic
AAGGTAACATTGAGAATATC+TGG 0.389748 7.2:-6192331 None:intergenic
CTGGTTCTGGTTAGCGGTAG+AGG 0.412467 7.2:-6191023 None:intergenic
GGGAATCGAACGTGTTCTTA+AGG 0.422078 7.2:+6191243 MS.gene07082:CDS
TAACCAGAACCAGTCTTCTC+CGG 0.444988 7.2:+6191033 MS.gene07082:CDS
TCAACCACAACTATGAAAAT+GGG 0.480712 7.2:+6191223 MS.gene07082:CDS
AGTATAATCCCGGAGAAGAC+TGG 0.483617 7.2:-6191042 None:intergenic
GGACCTGAGTCCATTGGATC+AGG 0.493608 7.2:+6192280 MS.gene07082:CDS
AACGAGATCGACGTTTGAAA+CGG 0.497051 7.2:-6191086 None:intergenic
AAATTCAAATTCAGTTTCGT+TGG 0.499228 7.2:-6190902 None:intergenic
AGGTGTTAAAGCTGCGATCA+AGG 0.501612 7.2:+6192300 MS.gene07082:CDS
GATGTTGTATCCGTTGAAAA+CGG 0.506210 7.2:+6191154 MS.gene07082:CDS
TTCATAGTTGTGGTTGAGCT+AGG 0.507201 7.2:-6191217 None:intergenic
AACGGTGAGGTCGAATTTCG+TGG 0.510902 7.2:-6191068 None:intergenic
CCTCCGTCAGAGATGAGGTA+AGG 0.518971 7.2:-6191124 None:intergenic
TATGTTGGACCTGAGTCCAT+TGG 0.522225 7.2:+6192274 MS.gene07082:CDS
CGCCCTTACCTCATCTCTGA+CGG 0.525777 7.2:+6191121 MS.gene07082:CDS
AGAAGACTGGTTCTGGTTAG+CGG 0.534911 7.2:-6191029 None:intergenic
AACCAGAACCAGTCTTCTCC+GGG 0.537520 7.2:+6191034 MS.gene07082:CDS
TTCGTGGCGGAGTATAATCC+CGG 0.541925 7.2:-6191052 None:intergenic
GTCGTTTCTCTTAAGCTACA+AGG 0.546334 7.2:+6191178 MS.gene07082:CDS
AATTGCTACCTAGGTGGAGA+AGG 0.556964 7.2:-6192350 None:intergenic
GACTGTGTTGTGGAATATGT+TGG 0.562518 7.2:+6192259 MS.gene07082:CDS
AGCAAGGGAAACAACGGTTG+AGG 0.563035 7.2:+6191318 MS.gene07082:CDS
TGAAGGTGGTGACTGTGTTG+TGG 0.573198 7.2:+6192249 MS.gene07082:CDS
AGCTTTAACACCTGATCCAA+TGG 0.583998 7.2:-6192290 None:intergenic
CTCCGTCAGAGATGAGGTAA+GGG 0.586257 7.2:-6191123 None:intergenic
CGTTTCCTCCGTCAGAGATG+AGG 0.588496 7.2:-6191129 None:intergenic
CAATGTTACCTTCTCCACCT+AGG 0.589162 7.2:+6192342 MS.gene07082:CDS
GGTTAGCGGTAGAGGATGAA+CGG 0.599940 7.2:-6191015 None:intergenic
TGTTTGATGATGATCAAGCA+AGG 0.620834 7.2:+6191302 MS.gene07082:CDS
GTTTGATGATGATCAAGCAA+GGG 0.625912 7.2:+6191303 MS.gene07082:CDS
CCTTACCTCATCTCTGACGG+AGG 0.627247 7.2:+6191124 MS.gene07082:CDS
TCATCTAGAGATACTGAGAC+CGG 0.630070 7.2:+6192186 MS.gene07082:CDS
ACACCTGATCCAATGGACTC+AGG 0.634250 7.2:-6192283 None:intergenic
GAATTGAATTGCTACCTAGG+TGG 0.663541 7.2:-6192356 None:intergenic
TGATCAAGCAAGGGAAACAA+CGG 0.689446 7.2:+6191312 MS.gene07082:CDS
GATCGACGTTTGAAACGGTG+AGG 0.719915 7.2:-6191081 None:intergenic
AGAGGATGAACGGATTACTG+TGG 0.730832 7.2:-6191005 None:intergenic
GGTGAGGTCGAATTTCGTGG+CGG 0.777998 7.2:-6191065 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTATTATTTATCTTTTA+TGG + chr7.2:6191369-6191388 MS.gene07082:intron 10.0%
!!! AATGTTTTTATAACCTATAT+TGG + chr7.2:6191650-6191669 MS.gene07082:intron 15.0%
!! AATAGTAAATGCAAAAAGTA+AGG - chr7.2:6191357-6191376 None:intergenic 20.0%
!! ATAGTAAATGCAAAAAGTAA+GGG - chr7.2:6191356-6191375 None:intergenic 20.0%
!! ATATTAGTTAACATCATGAA+AGG - chr7.2:6191515-6191534 None:intergenic 20.0%
!! TTGGATAAATAACTTATTTG+CGG + chr7.2:6191550-6191569 MS.gene07082:intron 20.0%
!!! TGATATTGTGAAATTGATTT+TGG - chr7.2:6192017-6192036 None:intergenic 20.0%
! AAATTCAAATTCAGTTTCGT+TGG - chr7.2:6190905-6190924 None:intergenic 25.0%
! TAGTAAATGCAAAAAGTAAG+GGG - chr7.2:6191355-6191374 None:intergenic 25.0%
! TGAAACCAAACATAAACTTA+GGG - chr7.2:6191772-6191791 None:intergenic 25.0%
! TGTTAACTAATATAGCAACT+AGG + chr7.2:6191522-6191541 MS.gene07082:intron 25.0%
! TTAGAATCAGTGTATGTTAT+TGG - chr7.2:6192073-6192092 None:intergenic 25.0%
!! ATGAATGTTAGTTGATGTTT+GGG + chr7.2:6192138-6192157 MS.gene07082:intron 25.0%
!! CTTTTATGGTTGTTAATGAT+TGG + chr7.2:6191383-6191402 MS.gene07082:intron 25.0%
!!! TTCCTTTTTAAGCTAGAAAT+TGG + chr7.2:6191887-6191906 MS.gene07082:intron 25.0%
AAGGTAACATTGAGAATATC+TGG - chr7.2:6192334-6192353 None:intergenic 30.0%
AGACCGGCAATTAAAAATTA+CGG + chr7.2:6192202-6192221 MS.gene07082:CDS 30.0%
CCTATATTGGAGAACTTATA+AGG + chr7.2:6191663-6191682 MS.gene07082:intron 30.0%
CCTTATAAGTTCTCCAATAT+AGG - chr7.2:6191666-6191685 None:intergenic 30.0%
GCTTAAAAAGGAATCAATTC+TGG - chr7.2:6191880-6191899 None:intergenic 30.0%
GTGAAACCAAACATAAACTT+AGG - chr7.2:6191773-6191792 None:intergenic 30.0%
TAAAAAGGAATCAATTCTGG+AGG - chr7.2:6191877-6191896 None:intergenic 30.0%
TCAACCACAACTATGAAAAT+GGG + chr7.2:6191223-6191242 MS.gene07082:CDS 30.0%
! GAAGTTCTTGAATTTTGCTT+TGG - chr7.2:6190983-6191002 None:intergenic 30.0%
! GATGAATGTTAGTTGATGTT+TGG + chr7.2:6192137-6192156 MS.gene07082:intron 30.0%
! TTCGTTGGTATTGAACAAAT+TGG - chr7.2:6190890-6190909 None:intergenic 30.0%
! TTTGGTTAAAAGTGAGTTGA+AGG - chr7.2:6191999-6192018 None:intergenic 30.0%
!! CAAAAGTGATTTTAAAGGTG+TGG + chr7.2:6191805-6191824 MS.gene07082:intron 30.0%
!! CTCCAATTTCTAGCTTAAAA+AGG - chr7.2:6191892-6191911 None:intergenic 30.0%
!!! CACACCTTTAAAATCACTTT+TGG - chr7.2:6191807-6191826 None:intergenic 30.0%
!!! TGAGATTGTGTTGTTTTTGT+TGG - chr7.2:6190934-6190953 None:intergenic 30.0%
ATATAGCAACTAGGTCTGTT+TGG + chr7.2:6191531-6191550 MS.gene07082:intron 35.0%
CACTTGCACGATATCTTTAA+TGG - chr7.2:6191284-6191303 None:intergenic 35.0%
CGTGTTCTTAAGGAGAAATT+TGG + chr7.2:6191253-6191272 MS.gene07082:CDS 35.0%
CTCAACCACAACTATGAAAA+TGG + chr7.2:6191222-6191241 MS.gene07082:CDS 35.0%
GATGTTGTATCCGTTGAAAA+CGG + chr7.2:6191154-6191173 MS.gene07082:CDS 35.0%
GTTGAAGGTAAACTGAATGA+TGG - chr7.2:6191984-6192003 None:intergenic 35.0%
! ATGTTTGGTTTCACTTCTAG+AGG + chr7.2:6191779-6191798 MS.gene07082:intron 35.0%
! GATTCCCATTTTCATAGTTG+TGG - chr7.2:6191230-6191249 None:intergenic 35.0%
! TGTTTGGTTTCACTTCTAGA+GGG + chr7.2:6191780-6191799 MS.gene07082:intron 35.0%
!! CAAGTTTTATCTGTTGAAGG+TGG + chr7.2:6192235-6192254 MS.gene07082:CDS 35.0%
!! CCTCCGTAATTTTTAATTGC+CGG - chr7.2:6192208-6192227 None:intergenic 35.0%
!! GTGCAAGTTTTATCTGTTGA+AGG + chr7.2:6192232-6192251 MS.gene07082:CDS 35.0%
!! GTTTGATGATGATCAAGCAA+GGG + chr7.2:6191303-6191322 MS.gene07082:CDS 35.0%
!! TGTTTGATGATGATCAAGCA+AGG + chr7.2:6191302-6191321 MS.gene07082:CDS 35.0%
AACGAGATCGACGTTTGAAA+CGG - chr7.2:6191089-6191108 None:intergenic 40.0%
AACTTAGGGCCAGTTTGAAT+TGG - chr7.2:6191758-6191777 None:intergenic 40.0%
ACTGGCCCTAAGTTTATGTT+TGG + chr7.2:6191764-6191783 MS.gene07082:intron 40.0%
CCGGCAATTAAAAATTACGG+AGG + chr7.2:6192205-6192224 MS.gene07082:CDS 40.0%
GACTGTGTTGTGGAATATGT+TGG + chr7.2:6192259-6192278 MS.gene07082:CDS 40.0%
GTCGTTTCTCTTAAGCTACA+AGG + chr7.2:6191178-6191197 MS.gene07082:CDS 40.0%
TCAGATACGCCAATTCAAAC+TGG + chr7.2:6191746-6191765 MS.gene07082:intron 40.0%
TCATCTAGAGATACTGAGAC+CGG + chr7.2:6192186-6192205 MS.gene07082:CDS 40.0%
TGATCAAGCAAGGGAAACAA+CGG + chr7.2:6191312-6191331 MS.gene07082:CDS 40.0%
TTCATAGTTGTGGTTGAGCT+AGG - chr7.2:6191220-6191239 None:intergenic 40.0%
! AGCTTTAACACCTGATCCAA+TGG - chr7.2:6192293-6192312 None:intergenic 40.0%
! GGGGCCAAAAGTGATTTTAA+AGG + chr7.2:6191800-6191819 MS.gene07082:intron 40.0%
! GTTAGTTGATGTTTGGGTGT+GGG + chr7.2:6192144-6192163 MS.gene07082:intron 40.0%
! GTTTGGTTTCACTTCTAGAG+GGG + chr7.2:6191781-6191800 MS.gene07082:intron 40.0%
! TGTTAGTTGATGTTTGGGTG+TGG + chr7.2:6192143-6192162 MS.gene07082:intron 40.0%
!!! TGTTTGGGTGTGGGTTTTTT+AGG + chr7.2:6192153-6192172 MS.gene07082:intron 40.0%
AGGTGTTAAAGCTGCGATCA+AGG + chr7.2:6192300-6192319 MS.gene07082:CDS 45.0%
AGTATAATCCCGGAGAAGAC+TGG - chr7.2:6191045-6191064 None:intergenic 45.0%
GAGCTCATCAATTGGCGTTT+GGG - chr7.2:6191728-6191747 None:intergenic 45.0%
GCGTATCTGAGCTCATCAAT+TGG - chr7.2:6191736-6191755 None:intergenic 45.0%
GGGAATCGAACGTGTTCTTA+AGG + chr7.2:6191243-6191262 MS.gene07082:CDS 45.0%
TAACCAGAACCAGTCTTCTC+CGG + chr7.2:6191033-6191052 MS.gene07082:CDS 45.0%
TATGTTGGACCTGAGTCCAT+TGG + chr7.2:6192274-6192293 MS.gene07082:CDS 45.0%
TGAGCTCATCAATTGGCGTT+TGG - chr7.2:6191729-6191748 None:intergenic 45.0%
!! AGAAACGACGCCGTTTTCAA+CGG - chr7.2:6191167-6191186 None:intergenic 45.0%
!! AGAAGACTGGTTCTGGTTAG+CGG - chr7.2:6191032-6191051 None:intergenic 45.0%
!! AGAGGATGAACGGATTACTG+TGG - chr7.2:6191008-6191027 None:intergenic 45.0%
!!! TTGGGTGTGGGTTTTTTAGG+CGG + chr7.2:6192156-6192175 MS.gene07082:intron 45.0%
AACCAGAACCAGTCTTCTCC+GGG + chr7.2:6191034-6191053 MS.gene07082:CDS 50.0%
AACGGTGAGGTCGAATTTCG+TGG - chr7.2:6191071-6191090 None:intergenic 50.0%
AGCAAGGGAAACAACGGTTG+AGG + chr7.2:6191318-6191337 MS.gene07082:CDS 50.0%
CTCCGTCAGAGATGAGGTAA+GGG - chr7.2:6191126-6191145 None:intergenic 50.0%
GGTTAGCGGTAGAGGATGAA+CGG - chr7.2:6191018-6191037 None:intergenic 50.0%
TGAAGGTGGTGACTGTGTTG+TGG + chr7.2:6192249-6192268 MS.gene07082:CDS 50.0%
! ACACCTGATCCAATGGACTC+AGG - chr7.2:6192286-6192305 None:intergenic 50.0%
! GATCGACGTTTGAAACGGTG+AGG - chr7.2:6191084-6191103 None:intergenic 50.0%
! TTCGTGGCGGAGTATAATCC+CGG - chr7.2:6191055-6191074 None:intergenic 50.0%
CCTCCGTCAGAGATGAGGTA+AGG - chr7.2:6191127-6191146 None:intergenic 55.0%
CCTTACCTCATCTCTGACGG+AGG + chr7.2:6191124-6191143 MS.gene07082:CDS 55.0%
CGCCCTTACCTCATCTCTGA+CGG + chr7.2:6191121-6191140 MS.gene07082:CDS 55.0%
CGTTTCCTCCGTCAGAGATG+AGG - chr7.2:6191132-6191151 None:intergenic 55.0%
GGACCTGAGTCCATTGGATC+AGG + chr7.2:6192280-6192299 MS.gene07082:CDS 55.0%
GGTGAGGTCGAATTTCGTGG+CGG - chr7.2:6191068-6191087 None:intergenic 55.0%
! ATCCCGGAGAAGACTGGTTC+TGG - chr7.2:6191039-6191058 None:intergenic 55.0%
!! CTGGTTCTGGTTAGCGGTAG+AGG - chr7.2:6191026-6191045 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 6190872 6192363 6190872 ID=MS.gene07082
chr7.2 mRNA 6190872 6192363 6190872 ID=MS.gene07082.t1;Parent=MS.gene07082
chr7.2 exon 6190872 6191339 6190872 ID=MS.gene07082.t1.exon1;Parent=MS.gene07082.t1
chr7.2 CDS 6190872 6191339 6190872 ID=cds.MS.gene07082.t1;Parent=MS.gene07082.t1
chr7.2 exon 6192175 6192363 6192175 ID=MS.gene07082.t1.exon2;Parent=MS.gene07082.t1
chr7.2 CDS 6192175 6192363 6192175 ID=cds.MS.gene07082.t1;Parent=MS.gene07082.t1
Gene Sequence

>MS.gene07082

ATGGCGACTCTAACTCCAATTTGTTCAATACCAACGAAACTGAATTTGAATTTGAGTATCCAACAAAAACAACACAATCTCAACTTCGCACTTTTCAATTTCAAAAATCCAAAGCAAAATTCAAGAACTTCAACCACAGTAATCCGTTCATCCTCTACCGCTAACCAGAACCAGTCTTCTCCGGGATTATACTCCGCCACGAAATTCGACCTCACCGTTTCAAACGTCGATCTCGTTCTCGAAGATGTTCGCCCTTACCTCATCTCTGACGGAGGAAACGTCGATGTTGTATCCGTTGAAAACGGCGTCGTTTCTCTTAAGCTACAAGGTGCATGCGAAAGTTGTCCTAGCTCAACCACAACTATGAAAATGGGAATCGAACGTGTTCTTAAGGAGAAATTTGGAGATTCCATTAAAGATATCGTGCAAGTGTTTGATGATGATCAAGCAAGGGAAACAACGGTTGAGGTCAGTGATTTTCCCCTTACTTTTTGCATTTACTATTATTTATCTTTTATGGTTGTTAATGATTGGATATTAATTTTAGTTAGTCTTATAGAAGTAGTTAGAAGTTAGATGTGATTAGTTGTTGTTAGTTAATTAGTTACATCACATATGAAGTGATCAATTTCAAAATCAACCTTTCATGATGTTAACTAATATAGCAACTAGGTCTGTTTGGATAAATAACTTATTTGCGGTTTATAGCACAATCACTTATAAGCTATTTTTGTAGCAAAAGATAAAATGAAGTTAAATTGTATTTGTATATGCTATAAATGTTTTTATAACCTATATTGGAGAACTTATAAGGTTAAGTTGTAAAAAGCTTATGAAAATTGTCGTAAGTTTTCCCAAACGCCAATTGATGAGCTCAGATACGCCAATTCAAACTGGCCCTAAGTTTATGTTTGGTTTCACTTCTAGAGGGGCCAAAAGTGATTTTAAAGGTGTGGAATTGATTTTGACATGTTTGATTACTCTCTACTATAATTGATTTTGCCTCCAGAATTGATTCCTTTTTAAGCTAGAAATTGGAGTTATAATTATAGAATTGATTTTAGCACTCAAATCTACTGTTCAACTCACTTTTACTTAAATGTAACAAACCATCATTCAGTTTACCTTCAACTCACTTTTAACCAAAATCAATTTCACAATATCAATTCATGCACAATCAATTTATGTCAATGCAAAACCAATAACATACACTGATTCTAATTAATATTCAATTTATCTAATGTGCATAGAAATGAAATGAAAATGATGAATGTTAGTTGATGTTTGGGTGTGGGTTTTTTAGGCGGTGAACAATCATCTAGAGATACTGAGACCGGCAATTAAAAATTACGGAGGTAGTGTGCAAGTTTTATCTGTTGAAGGTGGTGACTGTGTTGTGGAATATGTTGGACCTGAGTCCATTGGATCAGGTGTTAAAGCTGCGATCAAGGAGAAGTTTCCAGATATTCTCAATGTTACCTTCTCCACCTAG

Protein sequence

>MS.gene07082.t1

MATLTPICSIPTKLNLNLSIQQKQHNLNFALFNFKNPKQNSRTSTTVIRSSSTANQNQSSPGLYSATKFDLTVSNVDLVLEDVRPYLISDGGNVDVVSVENGVVSLKLQGACESCPSSTTTMKMGIERVLKEKFGDSIKDIVQVFDDDQARETTVEAVNNHLEILRPAIKNYGGSVQVLSVEGGDCVVEYVGPESIGSGVKAAIKEKFPDILNVTFST