AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95478.t1 MTR_5g045160 87.838 666 74 3 1 661 1 664 0.0 1158
MS.gene95478.t1 MTR_6g445440 80.938 661 101 5 1 661 1 636 0.0 1072
MS.gene95478.t1 MTR_3g103410 69.410 729 128 7 3 661 4 707 0.0 994
MS.gene95478.t1 MTR_5g045130 96.192 499 18 1 163 661 2 499 0.0 990
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95478.t1 AT5G13210 59.012 688 240 6 1 661 1 673 0.0 814
MS.gene95478.t1 AT5G43400 56.585 691 234 7 1 661 1 655 0.0 776
MS.gene95478.t1 AT5G43390 57.692 650 223 7 38 661 20 643 0.0 741
MS.gene95478.t1 AT3G24780 58.472 602 209 3 61 626 107 703 0.0 728

Find 215 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATTCCACTCTTCTTTC+TGG 0.042224 5.2:-35413905 None:intergenic
AGAATACGGTATAGAATTTC+AGG 0.195254 5.2:-35413858 None:intergenic
ATCATGAAGGAACTTAAAAT+TGG 0.217763 5.2:-35414177 None:intergenic
GCAGCTTCCATGGCTTCTTC+AGG 0.228556 5.2:-35415286 None:intergenic
GCCTCAGCTTCATGTGATTA+AGG 0.239712 5.2:+35414880 MS.gene95478:CDS
GTGTTGGCGGTGGTAGGGTT+AGG 0.239976 5.2:-35413420 None:intergenic
TCATGAGATTATAGAGTCTT+TGG 0.250372 5.2:+35414643 MS.gene95478:CDS
GCCTTCTTTGTCGGACTTGC+CGG 0.280706 5.2:-35413739 None:intergenic
ATATGCGATCAAACACCTTC+TGG 0.280967 5.2:-35414967 None:intergenic
ATATTAGAGGTGGCTGTTAA+TGG 0.287226 5.2:+35414986 MS.gene95478:CDS
AAATCAGTCTTGCTGCTAAA+TGG 0.287484 5.2:+35414270 MS.gene95478:CDS
GCCATGGAAGCTGCCATTGC+TGG 0.290034 5.2:+35415295 MS.gene95478:CDS
CTTCTCCTTATAAAACTTCA+TGG 0.297463 5.2:-35414531 None:intergenic
CTTGCTGTGTGTGATGTGTC+TGG 0.301225 5.2:+35414752 MS.gene95478:CDS
GACACAGTTTGTGAGGGATA+TGG 0.307219 5.2:+35414922 MS.gene95478:CDS
GTGTGATGTGTCTGGGAGTA+TGG 0.308226 5.2:+35414760 MS.gene95478:CDS
GCTTCTACGCCGCTGCTCTA+TGG 0.315868 5.2:+35413760 MS.gene95478:CDS
AGGAGGATCAGATGATTAAA+AGG 0.344113 5.2:+35415017 MS.gene95478:CDS
TACCGTATTCTCGAAGGTTC+TGG 0.347617 5.2:+35413870 MS.gene95478:CDS
GAGTTGCCTGAGGTGTTTAT+TGG 0.349776 5.2:+35414461 MS.gene95478:CDS
TCAACCCCATCGGTGGGTTT+CGG 0.356177 5.2:-35413542 None:intergenic
ATTCTTCTTCTTAAAGCAAA+AGG 0.361117 5.2:-35413969 None:intergenic
CAGCTTCCATGGCTTCTTCA+GGG 0.364076 5.2:-35415285 None:intergenic
CCTTTCTGAGTAGCCGGCAC+TGG 0.364582 5.2:-35415193 None:intergenic
GGGGTTTGATTCCTTACAAC+AGG 0.365521 5.2:+35414495 MS.gene95478:CDS
GTCTGTGGCATTGGGATTGT+TGG 0.368352 5.2:+35414802 MS.gene95478:CDS
AGGAGGCGCATTATGCTTAC+AGG 0.371898 5.2:+35414387 MS.gene95478:CDS
TGTTTATTGGTGCTAATCAA+TGG 0.374653 5.2:+35414474 MS.gene95478:CDS
GTCAGCGAGGGAAGGAATAT+TGG 0.378142 5.2:-35413814 None:intergenic
TGTTGGCGGTGGTAGGGTTA+GGG 0.384302 5.2:-35413419 None:intergenic
GGTCGCCAACTTCAACTCTC+TGG 0.384418 5.2:+35413512 MS.gene95478:CDS
CCTAGTGATTGCTTGGTAAT+CGG 0.388642 5.2:-35415095 None:intergenic
GGAGAGGTTTGAGAAGTACT+TGG 0.389397 5.2:+35414571 MS.gene95478:CDS
GTTTGTGAGGGATATGGATT+GGG 0.394363 5.2:+35414928 MS.gene95478:CDS
CTTTCTGAGTAGCCGGCACT+GGG 0.400130 5.2:-35415192 None:intergenic
TATTAGAGGTGGCTGTTAAT+GGG 0.405918 5.2:+35414987 MS.gene95478:CDS
TTCCAGAACCTTCGAGAATA+CGG 0.407578 5.2:-35413872 None:intergenic
CTTTGTGCTCCCTGTGGTTG+TGG 0.408732 5.2:-35414007 None:intergenic
CGATGTCTTCGTCGATCAAA+TGG 0.409403 5.2:+35413491 MS.gene95478:CDS
CTCCGTGGTGTTCGCGGTAC+CGG 0.409938 5.2:+35413720 MS.gene95478:CDS
GTTTATTGGTGCTAATCAAT+GGG 0.412509 5.2:+35414475 MS.gene95478:CDS
CATGAGATTATAGAGTCTTT+GGG 0.415849 5.2:+35414644 MS.gene95478:CDS
AATGGAGGTGTCTGTGGCAT+TGG 0.422690 5.2:+35414793 MS.gene95478:CDS
GGCGTAGAAGCCTTCTTTGT+CGG 0.425450 5.2:-35413748 None:intergenic
GAGTGGAATGAAAGGAAGTC+TGG 0.426054 5.2:+35413915 MS.gene95478:CDS
TGCTTGGTAATCGGTCTCCC+AGG 0.427130 5.2:-35415086 None:intergenic
CTGTTGACTCTTCCTTTGAT+CGG 0.430572 5.2:+35414300 MS.gene95478:CDS
CCTCAGCTTCATGTGATTAA+GGG 0.437072 5.2:+35414881 MS.gene95478:CDS
AGTGTCTCAGAGGGAGTATC+AGG 0.440493 5.2:-35413633 None:intergenic
ACAACTAGTTTCTGATACTC+AGG 0.440569 5.2:-35415319 None:intergenic
GGGAAGGAATATTGGTAGCT+AGG 0.448466 5.2:-35413806 None:intergenic
ATTCCTTCCCTCGCTGACTT+TGG 0.449943 5.2:+35413819 MS.gene95478:CDS
TTACAACAGGGTTGCTTCTG+TGG 0.450007 5.2:+35414508 MS.gene95478:CDS
GCCAGCAATGGCAGCTTCCA+TGG 0.450547 5.2:-35415296 None:intergenic
ATGCCACAGACACCTCCATT+GGG 0.450733 5.2:-35414790 None:intergenic
AGTTTGTGAGGGATATGGAT+TGG 0.451983 5.2:+35414927 MS.gene95478:CDS
ACTAGGAAGTATAGCGAGAA+AGG 0.454566 5.2:+35415112 MS.gene95478:CDS
ACAGGGTTCGTGATCGTTTG+AGG 0.461088 5.2:+35414405 MS.gene95478:CDS
ATGGAGGTGTCTGTGGCATT+GGG 0.462595 5.2:+35414794 MS.gene95478:CDS
GACTGAAGAGGTGGCGGCAA+GGG 0.464801 5.2:+35414064 MS.gene95478:CDS
CGTTGATGATTTGCTCAAGA+AGG 0.468757 5.2:+35414712 MS.gene95478:CDS
CTCAAGGGCCACCCCAGTGC+CGG 0.469365 5.2:+35415180 MS.gene95478:CDS
ATTATAGAGTCTTTGGGTGA+TGG 0.472199 5.2:+35414650 MS.gene95478:CDS
ATCAAGCGTCTATGAACCCC+TGG 0.476435 5.2:+35415068 MS.gene95478:CDS
GAAATCGAGACAAGGGTTAC+CGG 0.477245 5.2:-35413595 None:intergenic
TATACTTCCTAGTGATTGCT+TGG 0.477986 5.2:-35415102 None:intergenic
TCGTTAGTAGTTGTGGTGGT+GGG 0.483495 5.2:-35413471 None:intergenic
TTGCTGTGTGTGATGTGTCT+GGG 0.483780 5.2:+35414753 MS.gene95478:CDS
TCATGAAGGAACTTAAAATT+GGG 0.485493 5.2:-35414176 None:intergenic
TTCTTCTTCTTAAAGCAAAA+GGG 0.486069 5.2:-35413968 None:intergenic
AGTAGTTGTGGTGGTGGGGA+CGG 0.486738 5.2:-35413466 None:intergenic
AGAGGTGGCGGCAAGGGCTA+AGG 0.487539 5.2:+35414070 MS.gene95478:CDS
GTAATAACTTTCCCTTTCCA+AGG 0.490314 5.2:-35414845 None:intergenic
AATGCCACAGACACCTCCAT+TGG 0.491442 5.2:-35414791 None:intergenic
GCGATGGTGTTGGCGGTGGT+AGG 0.492641 5.2:-35413426 None:intergenic
ATCCCTCACAAACTGTGTCT+TGG 0.493125 5.2:-35414918 None:intergenic
TTCTCTGTCAACCCCATCGG+TGG 0.494614 5.2:-35413549 None:intergenic
AGAAAGAAGAGTGGAATGAA+AGG 0.498076 5.2:+35413907 MS.gene95478:CDS
GAATTGAGAGAGTAGATCTC+AGG 0.499359 5.2:-35413393 None:intergenic
ACAGAAGCAACCCTGTTGTA+AGG 0.500382 5.2:-35414506 None:intergenic
AGTTGTGGTGGTGGGGACGG+TGG 0.500957 5.2:-35413463 None:intergenic
GCTTGGTAATCGGTCTCCCA+GGG 0.503236 5.2:-35415085 None:intergenic
ATCGTTAGTAGTTGTGGTGG+TGG 0.504301 5.2:-35413472 None:intergenic
TGAGTGATGAACCTTGGAAA+GGG 0.505977 5.2:+35414834 MS.gene95478:CDS
TACTTGGAGGATGTGAAGGC+GGG 0.513737 5.2:+35414587 MS.gene95478:CDS
GGAATATCAAGGTGTGGAGG+AGG 0.513832 5.2:+35414370 MS.gene95478:CDS
GATGTGTCTGGGAGTATGGA+TGG 0.515466 5.2:+35414764 MS.gene95478:CDS
CTCTTCCTTCAAAACGTGAG+CGG 0.515554 5.2:-35414105 None:intergenic
GTATCAGAAACTAGTTGTGT+TGG 0.516169 5.2:+35415324 MS.gene95478:CDS
GGAAGGAATATTGGTAGCTA+GGG 0.519851 5.2:-35413805 None:intergenic
GAGGTTTGAGAAGTACTTGG+AGG 0.521875 5.2:+35414574 MS.gene95478:CDS
TGTAACCTCCGTGGTGTTCG+CGG 0.523569 5.2:+35413714 MS.gene95478:CDS
TACTCAGAAAGGAGTGGCGC+TGG 0.526512 5.2:+35415204 MS.gene95478:CDS
CGATGGTGTTGGCGGTGGTA+GGG 0.529027 5.2:-35413425 None:intergenic
GTCGCCAACTTCAACTCTCT+GGG 0.529325 5.2:+35413513 MS.gene95478:CDS
TGCGAAGAGGATCTTCCCTC+GGG 0.529868 5.2:+35414346 MS.gene95478:CDS
AAGTATAGCGAGAAAGGGTA+TGG 0.530567 5.2:+35415118 MS.gene95478:CDS
GCCACTCCTTTCTGAGTAGC+CGG 0.534013 5.2:-35415199 None:intergenic
GGTGTTTGATCGCATATTAG+AGG 0.534245 5.2:+35414973 MS.gene95478:CDS
GTTGATGATTTGCTCAAGAA+GGG 0.536020 5.2:+35414713 MS.gene95478:CDS
CGTTAGTAGTTGTGGTGGTG+GGG 0.537828 5.2:-35413470 None:intergenic
CCTTCCCAGAGAGTTGAAGT+TGG 0.541466 5.2:-35413517 None:intergenic
TGATGGAGATGGTGGAGAAG+TGG 0.544230 5.2:+35414667 MS.gene95478:CDS
TCGAGAGGCTCAAACTAGCT+TGG 0.545154 5.2:+35413658 MS.gene95478:CDS
CTCAGCTTCATGTGATTAAG+GGG 0.546670 5.2:+35414882 MS.gene95478:CDS
CCAGTGCCGGCTACTCAGAA+AGG 0.547181 5.2:+35415193 MS.gene95478:CDS
CTGAGTAGCCGGCACTGGGG+TGG 0.550129 5.2:-35415188 None:intergenic
TTGCGAAGAGGATCTTCCCT+CGG 0.551799 5.2:+35414345 MS.gene95478:CDS
GAGTATGGATGGGACCCCAA+TGG 0.560137 5.2:+35414775 MS.gene95478:CDS
CTGAATTGAGTGATGAACCT+TGG 0.560842 5.2:+35414828 MS.gene95478:CDS
GATCAGAGATAGAATCATGA+AGG 0.561689 5.2:-35414190 None:intergenic
CCGATTACCAAGCAATCACT+AGG 0.565104 5.2:+35415095 MS.gene95478:CDS
CTAGGAAGTATAGCGAGAAA+GGG 0.566439 5.2:+35415113 MS.gene95478:CDS
TTGGAATCTGAGAGACTCAA+GGG 0.568157 5.2:+35415165 MS.gene95478:CDS
TTGAGCGATGGTGTTGGCGG+TGG 0.569277 5.2:-35413430 None:intergenic
CTCTTTGATGACTGAAGAGG+TGG 0.569328 5.2:+35414055 MS.gene95478:CDS
GAGTCTTTGGGTGATGGAGA+TGG 0.570025 5.2:+35414656 MS.gene95478:CDS
ACCGGCAAGTCCGACAAAGA+AGG 0.570442 5.2:+35413738 MS.gene95478:CDS
ATGTTCTCTGTCAACCCCAT+CGG 0.575793 5.2:-35413552 None:intergenic
AAGGAACCGAAACCCACCGA+TGG 0.576154 5.2:+35413536 MS.gene95478:CDS
TGAGGGATATGGATTGGGGA+GGG 0.577292 5.2:+35414933 MS.gene95478:CDS
AGGAACCGAAACCCACCGAT+GGG 0.578305 5.2:+35413537 MS.gene95478:CDS
TTTGATGACTGAAGAGGTGG+CGG 0.579918 5.2:+35414058 MS.gene95478:CDS
GGGAAGACTACTATTGCTGC+TGG 0.580951 5.2:+35414608 MS.gene95478:CDS
GGAAGAGAAGAGGATTGCAT+TGG 0.580987 5.2:+35414121 MS.gene95478:CDS
CCCTTAATCACATGAAGCTG+AGG 0.581364 5.2:-35414881 None:intergenic
TTCCCTCGGGAGGAATATCA+AGG 0.581507 5.2:+35414359 MS.gene95478:CDS
AAGAAGAGTTGACCGATCAA+AGG 0.583361 5.2:-35414312 None:intergenic
TCAAAGGAAGAGTCAACAGA+AGG 0.584563 5.2:-35414296 None:intergenic
AAAGGAGTGGCGCTGGTGAG+TGG 0.584807 5.2:+35415211 MS.gene95478:CDS
TAACCAAAGTCAGCGAGGGA+AGG 0.585924 5.2:-35413822 None:intergenic
GTGAGGGATATGGATTGGGG+AGG 0.587167 5.2:+35414932 MS.gene95478:CDS
CTGATACTCAGGGCCAGCAA+TGG 0.588428 5.2:-35415308 None:intergenic
TCTTTGGGTGATGGAGATGG+TGG 0.591090 5.2:+35414659 MS.gene95478:CDS
CTCTCGACGAGTGTCTCAGA+GGG 0.592004 5.2:-35413642 None:intergenic
ATTCTATACCGTATTCTCGA+AGG 0.592026 5.2:+35413864 MS.gene95478:CDS
TCACCGACATTTCTCTCCAC+CGG 0.592355 5.2:+35413576 MS.gene95478:CDS
TTGAGAGAGTAGATCTCAGG+TGG 0.593722 5.2:-35413390 None:intergenic
GAAGACATCGTTAGTAGTTG+TGG 0.598692 5.2:-35413478 None:intergenic
AATAAGCCCTGAAGAAGCCA+TGG 0.598991 5.2:+35415279 MS.gene95478:CDS
GAATACGGTATAGAATTTCA+GGG 0.599290 5.2:-35413857 None:intergenic
TTTATTGGTGCTAATCAATG+GGG 0.599749 5.2:+35414476 MS.gene95478:CDS
TTGAGTGATGAACCTTGGAA+AGG 0.599784 5.2:+35414833 MS.gene95478:CDS
GTTACAGACGAGTTTGAGAG+TGG 0.603374 5.2:-35413697 None:intergenic
TGACTGAAGAGGTGGCGGCA+AGG 0.603645 5.2:+35414063 MS.gene95478:CDS
TCTCTGTCAACCCCATCGGT+GGG 0.605392 5.2:-35413548 None:intergenic
TTTGGAATCTGAGAGACTCA+AGG 0.605497 5.2:+35415164 MS.gene95478:CDS
ACACTGTTCTTAGATAATGA+AGG 0.606413 5.2:+35415253 MS.gene95478:CDS
GAGAAGTGGCTGAGTTGCAG+TGG 0.607162 5.2:+35414681 MS.gene95478:CDS
GGCTGTTAATGGGAATCTCA+AGG 0.608020 5.2:+35414997 MS.gene95478:CDS
CGAGTTTGAGAGTGGTGAGT+GGG 0.609278 5.2:-35413689 None:intergenic
ATGGCCGCCGTCACTATCGT+TGG 0.610913 5.2:+35413366 MS.gene95478:CDS
CACCTTGATATTCCTCCCGA+GGG 0.610961 5.2:-35414361 None:intergenic
ATGTGTCTGGGAGTATGGAT+GGG 0.612295 5.2:+35414765 MS.gene95478:CDS
AAGATGTGTTAGTGCCGTTG+AGG 0.614181 5.2:+35414429 MS.gene95478:CDS
GAAGTACTTGGAGGATGTGA+AGG 0.615842 5.2:+35414583 MS.gene95478:CDS
AGTCCAAGACACAGTTTGTG+AGG 0.618235 5.2:+35414915 MS.gene95478:CDS
AGACTCTTTGATGACTGAAG+AGG 0.619882 5.2:+35414052 MS.gene95478:CDS
TTTGTGAGACAATTGCGAAG+AGG 0.621995 5.2:+35414333 MS.gene95478:CDS
GTTTGATCGCATATTAGAGG+TGG 0.624316 5.2:+35414976 MS.gene95478:CDS
TTTGTGAGGGATATGGATTG+GGG 0.624744 5.2:+35414929 MS.gene95478:CDS
TGTGTTAGTGCCGTTGAGGA+AGG 0.624791 5.2:+35414433 MS.gene95478:CDS
ACACCTTGATATTCCTCCCG+AGG 0.626089 5.2:-35414362 None:intergenic
TTAGCACCAATAAACACCTC+AGG 0.626398 5.2:-35414467 None:intergenic
CCAACTTCAACTCTCTGGGA+AGG 0.627220 5.2:+35413517 MS.gene95478:CDS
AGTGCCGTTGAGGAAGGTGT+TGG 0.627511 5.2:+35414439 MS.gene95478:CDS
GTACTTGGAGGATGTGAAGG+CGG 0.628790 5.2:+35414586 MS.gene95478:CDS
ACAGGGAGCACAAAGGTGCG+TGG 0.629027 5.2:+35414015 MS.gene95478:CDS
AACTCCAACACCTTCCTCAA+CGG 0.630137 5.2:-35414443 None:intergenic
GACATCGTTAGTAGTTGTGG+TGG 0.630685 5.2:-35413475 None:intergenic
TTACCGGTGGAGAGAAATGT+CGG 0.631186 5.2:-35413579 None:intergenic
GACCCCAATGGAGGTGTCTG+TGG 0.633142 5.2:+35414787 MS.gene95478:CDS
GATCTTCGTATTCAGTGACA+TGG 0.634847 5.2:+35415039 MS.gene95478:CDS
AAAACAACGCCACAACCACA+GGG 0.637769 5.2:+35413998 MS.gene95478:CDS
GTGAAATCCAGAAAGAAGAG+TGG 0.638332 5.2:+35413898 MS.gene95478:CDS
TATGGATGGGACCCCAATGG+AGG 0.639461 5.2:+35414778 MS.gene95478:CDS
ACGAGTTTGAGAGTGGTGAG+TGG 0.639671 5.2:-35413690 None:intergenic
ACTTGGAGGATGTGAAGGCG+GGG 0.639940 5.2:+35414588 MS.gene95478:CDS
CCTCTCGACGAGTGTCTCAG+AGG 0.640219 5.2:-35413643 None:intergenic
GAAGTAACCAAAGTCAGCGA+GGG 0.641029 5.2:-35413826 None:intergenic
GTCCAAGACACAGTTTGTGA+GGG 0.641648 5.2:+35414916 MS.gene95478:CDS
CAAAGGAAGAGTCAACAGAA+GGG 0.642577 5.2:-35414295 None:intergenic
AAAGACTCTATAATCTCATG+AGG 0.642725 5.2:-35414641 None:intergenic
GGAAGAAGAAATCGAGACAA+GGG 0.645821 5.2:-35413602 None:intergenic
GAAGGTGTTGGAGTTGCCTG+AGG 0.648237 5.2:+35414451 MS.gene95478:CDS
GGAGGCGCATTATGCTTACA+GGG 0.649659 5.2:+35414388 MS.gene95478:CDS
AGGTGGGCCAACGATAGTGA+CGG 0.650069 5.2:-35413373 None:intergenic
TTCATGAAACCATAGAGCAG+CGG 0.650549 5.2:-35413769 None:intergenic
CAACTAGTTTCTGATACTCA+GGG 0.653189 5.2:-35415318 None:intergenic
GCCGGCTACTCAGAAAGGAG+TGG 0.654091 5.2:+35415198 MS.gene95478:CDS
CCTCTGAGACACTCGTCGAG+AGG 0.656154 5.2:+35413643 MS.gene95478:CDS
TCGGGAGGAATATCAAGGTG+TGG 0.659458 5.2:+35414364 MS.gene95478:CDS
TCAAGCGTCTATGAACCCCT+GGG 0.661453 5.2:+35415069 MS.gene95478:CDS
TGGAAGAAGAAATCGAGACA+AGG 0.663800 5.2:-35413603 None:intergenic
AGGGAACACTGATTTCCAGA+AGG 0.666142 5.2:+35414952 MS.gene95478:CDS
AGGGAGTATCAGGAACAACA+TGG 0.666736 5.2:-35413623 None:intergenic
GGGTTTGATTCCTTACAACA+GGG 0.668689 5.2:+35414496 MS.gene95478:CDS
ACGCACCTTTGTGCTCCCTG+TGG 0.671174 5.2:-35414013 None:intergenic
ATCGAGACAAGGGTTACCGG+TGG 0.672928 5.2:-35413592 None:intergenic
GAAAGTTATTACTTTCAGCG+AGG 0.676634 5.2:+35414856 MS.gene95478:CDS
CACAACCACAGGGAGCACAA+AGG 0.677205 5.2:+35414008 MS.gene95478:CDS
CTTGGTAATCGGTCTCCCAG+GGG 0.678476 5.2:-35415084 None:intergenic
TGCCACAGACACCTCCATTG+GGG 0.683762 5.2:-35414789 None:intergenic
GGAGGAATATCAAGGTGTGG+AGG 0.686729 5.2:+35414367 MS.gene95478:CDS
TGAAGTAACCAAAGTCAGCG+AGG 0.688387 5.2:-35413827 None:intergenic
GAAGAGGATCTTCCCTCGGG+AGG 0.699485 5.2:+35414349 MS.gene95478:CDS
TGCCGGTACCGCGAACACCA+CGG 0.705237 5.2:-35413722 None:intergenic
TCAGCTTCATGTGATTAAGG+GGG 0.710103 5.2:+35414883 MS.gene95478:CDS
TGGGCCAACGATAGTGACGG+CGG 0.711312 5.2:-35413370 None:intergenic
TGAGAGAGTAGATCTCAGGT+GGG 0.716996 5.2:-35413389 None:intergenic
GGAACCGAAACCCACCGATG+GGG 0.722058 5.2:+35413538 MS.gene95478:CDS
AAACTCGTCTGTAACCTCCG+TGG 0.731806 5.2:+35413705 MS.gene95478:CDS
TGTTAATGGGAATCTCAAGG+AGG 0.769174 5.2:+35415000 MS.gene95478:CDS
TTTCTGAGTAGCCGGCACTG+GGG 0.795157 5.2:-35415191 None:intergenic
CGGTACCGCGAACACCACGG+AGG 0.799645 5.2:-35413719 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCATGAAGGAACTTAAAAT+TGG - chr5.2:35414180-35414199 None:intergenic 25.0%
! ATTCTTCTTCTTAAAGCAAA+AGG - chr5.2:35413972-35413991 None:intergenic 25.0%
! TCATGAAGGAACTTAAAATT+GGG - chr5.2:35414179-35414198 None:intergenic 25.0%
! TTCTTCTTCTTAAAGCAAAA+GGG - chr5.2:35413971-35413990 None:intergenic 25.0%
AAAGACTCTATAATCTCATG+AGG - chr5.2:35414644-35414663 None:intergenic 30.0%
ACACTGTTCTTAGATAATGA+AGG + chr5.2:35415253-35415272 MS.gene95478:CDS 30.0%
AGAATACGGTATAGAATTTC+AGG - chr5.2:35413861-35413880 None:intergenic 30.0%
AGATTCCAAAAAACTATCTG+AGG - chr5.2:35415154-35415173 None:intergenic 30.0%
CATGAGATTATAGAGTCTTT+GGG + chr5.2:35414644-35414663 MS.gene95478:CDS 30.0%
CTTCTCCTTATAAAACTTCA+TGG - chr5.2:35414534-35414553 None:intergenic 30.0%
GAATACGGTATAGAATTTCA+GGG - chr5.2:35413860-35413879 None:intergenic 30.0%
TCATGAGATTATAGAGTCTT+TGG + chr5.2:35414643-35414662 MS.gene95478:CDS 30.0%
!! GTTTATTGGTGCTAATCAAT+GGG + chr5.2:35414475-35414494 MS.gene95478:CDS 30.0%
!! TGTTTATTGGTGCTAATCAA+TGG + chr5.2:35414474-35414493 MS.gene95478:CDS 30.0%
!! TTTATTGGTGCTAATCAATG+GGG + chr5.2:35414476-35414495 MS.gene95478:CDS 30.0%
!! TTTTGCTGATTGTTTGAAGA+AGG + chr5.2:35414214-35414233 MS.gene95478:CDS 30.0%
!!! ATCTCGAGTTTTTGAAATCT+GGG + chr5.2:35414237-35414256 MS.gene95478:CDS 30.0%
!!! GAAGTTTTTGAAGCATGATA+AGG + chr5.2:35414550-35414569 MS.gene95478:CDS 30.0%
AAATCAGTCTTGCTGCTAAA+TGG + chr5.2:35414270-35414289 MS.gene95478:CDS 35.0%
AGAAAGAAGAGTGGAATGAA+AGG + chr5.2:35413907-35413926 MS.gene95478:CDS 35.0%
AGGAGGATCAGATGATTAAA+AGG + chr5.2:35415017-35415036 MS.gene95478:CDS 35.0%
ATATTAGAGGTGGCTGTTAA+TGG + chr5.2:35414986-35415005 MS.gene95478:CDS 35.0%
ATTATAGAGTCTTTGGGTGA+TGG + chr5.2:35414650-35414669 MS.gene95478:CDS 35.0%
ATTCTATACCGTATTCTCGA+AGG + chr5.2:35413864-35413883 MS.gene95478:CDS 35.0%
GAAAGTTATTACTTTCAGCG+AGG + chr5.2:35414856-35414875 MS.gene95478:CDS 35.0%
GATCAGAGATAGAATCATGA+AGG - chr5.2:35414193-35414212 None:intergenic 35.0%
GTAATAACTTTCCCTTTCCA+AGG - chr5.2:35414848-35414867 None:intergenic 35.0%
GTATCAGAAACTAGTTGTGT+TGG + chr5.2:35415324-35415343 MS.gene95478:CDS 35.0%
TATACTTCCTAGTGATTGCT+TGG - chr5.2:35415105-35415124 None:intergenic 35.0%
TATTAGAGGTGGCTGTTAAT+GGG + chr5.2:35414987-35415006 MS.gene95478:CDS 35.0%
TTTCATTCCACTCTTCTTTC+TGG - chr5.2:35413908-35413927 None:intergenic 35.0%
! ACAACTAGTTTCTGATACTC+AGG - chr5.2:35415322-35415341 None:intergenic 35.0%
! CAACTAGTTTCTGATACTCA+GGG - chr5.2:35415321-35415340 None:intergenic 35.0%
!! ATATTGGTAGCTAGGGTTTT+AGG - chr5.2:35413801-35413820 None:intergenic 35.0%
!! GTTGATGATTTGCTCAAGAA+GGG + chr5.2:35414713-35414732 MS.gene95478:CDS 35.0%
!! TATTGGTAGCTAGGGTTTTA+GGG - chr5.2:35413800-35413819 None:intergenic 35.0%
!! TGTGGCCATGAAGTTTTATA+AGG + chr5.2:35414526-35414545 MS.gene95478:CDS 35.0%
!! TTGTTGCAAGCGCTTATTTT+TGG - chr5.2:35413943-35413962 None:intergenic 35.0%
!!! GATCTCGAGTTTTTGAAATC+TGG + chr5.2:35414236-35414255 MS.gene95478:CDS 35.0%
!!! TTGTGCCTCAGATAGTTTTT+TGG + chr5.2:35415146-35415165 MS.gene95478:CDS 35.0%
!!! TTTTGAAGCATGATAAGGAG+AGG + chr5.2:35414555-35414574 MS.gene95478:CDS 35.0%
AAGAAGAGTTGACCGATCAA+AGG - chr5.2:35414315-35414334 None:intergenic 40.0%
AAGTATAGCGAGAAAGGGTA+TGG + chr5.2:35415118-35415137 MS.gene95478:CDS 40.0%
AGTTTGTGAGGGATATGGAT+TGG + chr5.2:35414927-35414946 MS.gene95478:CDS 40.0%
ATATGCGATCAAACACCTTC+TGG - chr5.2:35414970-35414989 None:intergenic 40.0%
CAAAGGAAGAGTCAACAGAA+GGG - chr5.2:35414298-35414317 None:intergenic 40.0%
CCTCAGCTTCATGTGATTAA+GGG + chr5.2:35414881-35414900 MS.gene95478:CDS 40.0%
CTCAGCTTCATGTGATTAAG+GGG + chr5.2:35414882-35414901 MS.gene95478:CDS 40.0%
CTGAATTGAGTGATGAACCT+TGG + chr5.2:35414828-35414847 MS.gene95478:CDS 40.0%
GAAGACATCGTTAGTAGTTG+TGG - chr5.2:35413481-35413500 None:intergenic 40.0%
GAATTGAGAGAGTAGATCTC+AGG - chr5.2:35413396-35413415 None:intergenic 40.0%
GATCTTCGTATTCAGTGACA+TGG + chr5.2:35415039-35415058 MS.gene95478:CDS 40.0%
GGAAGAAGAAATCGAGACAA+GGG - chr5.2:35413605-35413624 None:intergenic 40.0%
GGGTTTGATTCCTTACAACA+GGG + chr5.2:35414496-35414515 MS.gene95478:CDS 40.0%
GGTGTTTGATCGCATATTAG+AGG + chr5.2:35414973-35414992 MS.gene95478:CDS 40.0%
GTGAAATCCAGAAAGAAGAG+TGG + chr5.2:35413898-35413917 MS.gene95478:CDS 40.0%
GTTTGATCGCATATTAGAGG+TGG + chr5.2:35414976-35414995 MS.gene95478:CDS 40.0%
GTTTGTGAGGGATATGGATT+GGG + chr5.2:35414928-35414947 MS.gene95478:CDS 40.0%
TCAAAGGAAGAGTCAACAGA+AGG - chr5.2:35414299-35414318 None:intergenic 40.0%
TCAGCTTCATGTGATTAAGG+GGG + chr5.2:35414883-35414902 MS.gene95478:CDS 40.0%
TGAGTGATGAACCTTGGAAA+GGG + chr5.2:35414834-35414853 MS.gene95478:CDS 40.0%
TGGAAGAAGAAATCGAGACA+AGG - chr5.2:35413606-35413625 None:intergenic 40.0%
TGTTAATGGGAATCTCAAGG+AGG + chr5.2:35415000-35415019 MS.gene95478:CDS 40.0%
TTAGCACCAATAAACACCTC+AGG - chr5.2:35414470-35414489 None:intergenic 40.0%
TTCATGAAACCATAGAGCAG+CGG - chr5.2:35413772-35413791 None:intergenic 40.0%
TTCCAGAACCTTCGAGAATA+CGG - chr5.2:35413875-35413894 None:intergenic 40.0%
TTGAGTGATGAACCTTGGAA+AGG + chr5.2:35414833-35414852 MS.gene95478:CDS 40.0%
TTGGAATCTGAGAGACTCAA+GGG + chr5.2:35415165-35415184 MS.gene95478:CDS 40.0%
TTTGTGAGACAATTGCGAAG+AGG + chr5.2:35414333-35414352 MS.gene95478:CDS 40.0%
! ACTAGGAAGTATAGCGAGAA+AGG + chr5.2:35415112-35415131 MS.gene95478:CDS 40.0%
! AGACTCTTTGATGACTGAAG+AGG + chr5.2:35414052-35414071 MS.gene95478:CDS 40.0%
! CCTAGTGATTGCTTGGTAAT+CGG - chr5.2:35415098-35415117 None:intergenic 40.0%
! CTAGGAAGTATAGCGAGAAA+GGG + chr5.2:35415113-35415132 MS.gene95478:CDS 40.0%
! GGAAGGAATATTGGTAGCTA+GGG - chr5.2:35413808-35413827 None:intergenic 40.0%
! TTTGGAATCTGAGAGACTCA+AGG + chr5.2:35415164-35415183 MS.gene95478:CDS 40.0%
! TTTGTGAGGGATATGGATTG+GGG + chr5.2:35414929-35414948 MS.gene95478:CDS 40.0%
!! AGCAGCAAGACTGATTTTTG+TGG - chr5.2:35414267-35414286 None:intergenic 40.0%
!! CGTTGATGATTTGCTCAAGA+AGG + chr5.2:35414712-35414731 MS.gene95478:CDS 40.0%
!! CTGTTGACTCTTCCTTTGAT+CGG + chr5.2:35414300-35414319 MS.gene95478:CDS 40.0%
!! GCAGCAAGACTGATTTTTGT+GGG - chr5.2:35414266-35414285 None:intergenic 40.0%
!!! ACGTTTTGAAGGAAGAGAAG+AGG + chr5.2:35414111-35414130 MS.gene95478:CDS 40.0%
!!! AGTTTTTTGAGCGATGGTGT+TGG - chr5.2:35413439-35413458 None:intergenic 40.0%
!!! GGTTTCAGTTTTTTGAGCGA+TGG - chr5.2:35413445-35413464 None:intergenic 40.0%
AAAAACAACGCCACAACCAC+AGG + chr5.2:35413997-35414016 MS.gene95478:CDS 45.0%
AAAACAACGCCACAACCACA+GGG + chr5.2:35413998-35414017 MS.gene95478:CDS 45.0%
AACTCCAACACCTTCCTCAA+CGG - chr5.2:35414446-35414465 None:intergenic 45.0%
AATAAGCCCTGAAGAAGCCA+TGG + chr5.2:35415279-35415298 MS.gene95478:CDS 45.0%
ACAGAAGCAACCCTGTTGTA+AGG - chr5.2:35414509-35414528 None:intergenic 45.0%
AGTCCAAGACACAGTTTGTG+AGG + chr5.2:35414915-35414934 MS.gene95478:CDS 45.0%
ATCCCTCACAAACTGTGTCT+TGG - chr5.2:35414921-35414940 None:intergenic 45.0%
ATCGTTAGTAGTTGTGGTGG+TGG - chr5.2:35413475-35413494 None:intergenic 45.0%
ATGTTCTCTGTCAACCCCAT+CGG - chr5.2:35413555-35413574 None:intergenic 45.0%
CCCTTAATCACATGAAGCTG+AGG - chr5.2:35414884-35414903 None:intergenic 45.0%
CCGATTACCAAGCAATCACT+AGG + chr5.2:35415095-35415114 MS.gene95478:CDS 45.0%
CGATGTCTTCGTCGATCAAA+TGG + chr5.2:35413491-35413510 MS.gene95478:CDS 45.0%
CTCTTCCTTCAAAACGTGAG+CGG - chr5.2:35414108-35414127 None:intergenic 45.0%
GAAATCGAGACAAGGGTTAC+CGG - chr5.2:35413598-35413617 None:intergenic 45.0%
GAAGTAACCAAAGTCAGCGA+GGG - chr5.2:35413829-35413848 None:intergenic 45.0%
GACACAGTTTGTGAGGGATA+TGG + chr5.2:35414922-35414941 MS.gene95478:CDS 45.0%
GACATCGTTAGTAGTTGTGG+TGG - chr5.2:35413478-35413497 None:intergenic 45.0%
GAGTGGAATGAAAGGAAGTC+TGG + chr5.2:35413915-35413934 MS.gene95478:CDS 45.0%
GCCTCAGCTTCATGTGATTA+AGG + chr5.2:35414880-35414899 MS.gene95478:CDS 45.0%
GGAAGAGAAGAGGATTGCAT+TGG + chr5.2:35414121-35414140 MS.gene95478:CDS 45.0%
GGCTGTTAATGGGAATCTCA+AGG + chr5.2:35414997-35415016 MS.gene95478:CDS 45.0%
GGGGTTTGATTCCTTACAAC+AGG + chr5.2:35414495-35414514 MS.gene95478:CDS 45.0%
GTCCAAGACACAGTTTGTGA+GGG + chr5.2:35414916-35414935 MS.gene95478:CDS 45.0%
GTTACAGACGAGTTTGAGAG+TGG - chr5.2:35413700-35413719 None:intergenic 45.0%
TACCGTATTCTCGAAGGTTC+TGG + chr5.2:35413870-35413889 MS.gene95478:CDS 45.0%
TCGTTAGTAGTTGTGGTGGT+GGG - chr5.2:35413474-35413493 None:intergenic 45.0%
TGAAGTAACCAAAGTCAGCG+AGG - chr5.2:35413830-35413849 None:intergenic 45.0%
TGAGAGAGTAGATCTCAGGT+GGG - chr5.2:35413392-35413411 None:intergenic 45.0%
TTACAACAGGGTTGCTTCTG+TGG + chr5.2:35414508-35414527 MS.gene95478:CDS 45.0%
TTACCGGTGGAGAGAAATGT+CGG - chr5.2:35413582-35413601 None:intergenic 45.0%
TTGAGAGAGTAGATCTCAGG+TGG - chr5.2:35413393-35413412 None:intergenic 45.0%
TTGCTGTGTGTGATGTGTCT+GGG + chr5.2:35414753-35414772 MS.gene95478:CDS 45.0%
! AAGATGTGTTAGTGCCGTTG+AGG + chr5.2:35414429-35414448 MS.gene95478:CDS 45.0%
! ACAAACCGCTCACGTTTTGA+AGG + chr5.2:35414100-35414119 MS.gene95478:CDS 45.0%
! ATGTGTCTGGGAGTATGGAT+GGG + chr5.2:35414765-35414784 MS.gene95478:CDS 45.0%
! CTCTTTGATGACTGAAGAGG+TGG + chr5.2:35414055-35414074 MS.gene95478:CDS 45.0%
! GAAGTACTTGGAGGATGTGA+AGG + chr5.2:35414583-35414602 MS.gene95478:CDS 45.0%
! GAGTTGCCTGAGGTGTTTAT+TGG + chr5.2:35414461-35414480 MS.gene95478:CDS 45.0%
! GGGAAGGAATATTGGTAGCT+AGG - chr5.2:35413809-35413828 None:intergenic 45.0%
! TTTGATGACTGAAGAGGTGG+CGG + chr5.2:35414058-35414077 MS.gene95478:CDS 45.0%
!! AGGGAACACTGATTTCCAGA+AGG + chr5.2:35414952-35414971 MS.gene95478:CDS 45.0%
!! AGGGAGTATCAGGAACAACA+TGG - chr5.2:35413626-35413645 None:intergenic 45.0%
!! GAGGTTTGAGAAGTACTTGG+AGG + chr5.2:35414574-35414593 MS.gene95478:CDS 45.0%
!! GGAGAGGTTTGAGAAGTACT+TGG + chr5.2:35414571-35414590 MS.gene95478:CDS 45.0%
!! TTTTTGAGCGATGGTGTTGG+CGG - chr5.2:35413436-35413455 None:intergenic 45.0%
AAACTCGTCTGTAACCTCCG+TGG + chr5.2:35413705-35413724 MS.gene95478:CDS 50.0%
AATGCCACAGACACCTCCAT+TGG - chr5.2:35414794-35414813 None:intergenic 50.0%
ACACCTTGATATTCCTCCCG+AGG - chr5.2:35414365-35414384 None:intergenic 50.0%
ACAGGGTTCGTGATCGTTTG+AGG + chr5.2:35414405-35414424 MS.gene95478:CDS 50.0%
ACGAGTTTGAGAGTGGTGAG+TGG - chr5.2:35413693-35413712 None:intergenic 50.0%
ATCAAGCGTCTATGAACCCC+TGG + chr5.2:35415068-35415087 MS.gene95478:CDS 50.0%
ATGCCACAGACACCTCCATT+GGG - chr5.2:35414793-35414812 None:intergenic 50.0%
CACCTTGATATTCCTCCCGA+GGG - chr5.2:35414364-35414383 None:intergenic 50.0%
CAGCTTCCATGGCTTCTTCA+GGG - chr5.2:35415288-35415307 None:intergenic 50.0%
CCTTCCCAGAGAGTTGAAGT+TGG - chr5.2:35413520-35413539 None:intergenic 50.0%
CGAGTTTGAGAGTGGTGAGT+GGG - chr5.2:35413692-35413711 None:intergenic 50.0%
GGAATATCAAGGTGTGGAGG+AGG + chr5.2:35414370-35414389 MS.gene95478:CDS 50.0%
GGAGGAATATCAAGGTGTGG+AGG + chr5.2:35414367-35414386 MS.gene95478:CDS 50.0%
GGCGTAGAAGCCTTCTTTGT+CGG - chr5.2:35413751-35413770 None:intergenic 50.0%
GGGAAGACTACTATTGCTGC+TGG + chr5.2:35414608-35414627 MS.gene95478:CDS 50.0%
GTACTTGGAGGATGTGAAGG+CGG + chr5.2:35414586-35414605 MS.gene95478:CDS 50.0%
GTCAGCGAGGGAAGGAATAT+TGG - chr5.2:35413817-35413836 None:intergenic 50.0%
GTCGCCAACTTCAACTCTCT+GGG + chr5.2:35413513-35413532 MS.gene95478:CDS 50.0%
GTGTGATGTGTCTGGGAGTA+TGG + chr5.2:35414760-35414779 MS.gene95478:CDS 50.0%
TAACCAAAGTCAGCGAGGGA+AGG - chr5.2:35413825-35413844 None:intergenic 50.0%
TACTTGGAGGATGTGAAGGC+GGG + chr5.2:35414587-35414606 MS.gene95478:CDS 50.0%
TCAAGCGTCTATGAACCCCT+GGG + chr5.2:35415069-35415088 MS.gene95478:CDS 50.0%
TCACCGACATTTCTCTCCAC+CGG + chr5.2:35413576-35413595 MS.gene95478:CDS 50.0%
TCGAGAGGCTCAAACTAGCT+TGG + chr5.2:35413658-35413677 MS.gene95478:CDS 50.0%
TCGGGAGGAATATCAAGGTG+TGG + chr5.2:35414364-35414383 MS.gene95478:CDS 50.0%
TTCCCTCGGGAGGAATATCA+AGG + chr5.2:35414359-35414378 MS.gene95478:CDS 50.0%
TTGCGAAGAGGATCTTCCCT+CGG + chr5.2:35414345-35414364 MS.gene95478:CDS 50.0%
! AGGAGGCGCATTATGCTTAC+AGG + chr5.2:35414387-35414406 MS.gene95478:CDS 50.0%
! CCAACTTCAACTCTCTGGGA+AGG + chr5.2:35413517-35413536 MS.gene95478:CDS 50.0%
! CGTTAGTAGTTGTGGTGGTG+GGG - chr5.2:35413473-35413492 None:intergenic 50.0%
! CTTGCTGTGTGTGATGTGTC+TGG + chr5.2:35414752-35414771 MS.gene95478:CDS 50.0%
! GATGTGTCTGGGAGTATGGA+TGG + chr5.2:35414764-35414783 MS.gene95478:CDS 50.0%
! GGAGGCGCATTATGCTTACA+GGG + chr5.2:35414388-35414407 MS.gene95478:CDS 50.0%
! GTCTGTGGCATTGGGATTGT+TGG + chr5.2:35414802-35414821 MS.gene95478:CDS 50.0%
! TGTGTTAGTGCCGTTGAGGA+AGG + chr5.2:35414433-35414452 MS.gene95478:CDS 50.0%
!! AATGGAGGTGTCTGTGGCAT+TGG + chr5.2:35414793-35414812 MS.gene95478:CDS 50.0%
!! AGTGTCTCAGAGGGAGTATC+AGG - chr5.2:35413636-35413655 None:intergenic 50.0%
!! ATGGAGGTGTCTGTGGCATT+GGG + chr5.2:35414794-35414813 MS.gene95478:CDS 50.0%
!! ATTCCTTCCCTCGCTGACTT+TGG + chr5.2:35413819-35413838 MS.gene95478:CDS 50.0%
!! GAGTCTTTGGGTGATGGAGA+TGG + chr5.2:35414656-35414675 MS.gene95478:CDS 50.0%
!! TCTTTGGGTGATGGAGATGG+TGG + chr5.2:35414659-35414678 MS.gene95478:CDS 50.0%
!! TGAGGGATATGGATTGGGGA+GGG + chr5.2:35414933-35414952 MS.gene95478:CDS 50.0%
!! TGATGGAGATGGTGGAGAAG+TGG + chr5.2:35414667-35414686 MS.gene95478:CDS 50.0%
AAGGAACCGAAACCCACCGA+TGG + chr5.2:35413536-35413555 MS.gene95478:CDS 55.0%
ACCGGCAAGTCCGACAAAGA+AGG + chr5.2:35413738-35413757 MS.gene95478:CDS 55.0%
ACTTGGAGGATGTGAAGGCG+GGG + chr5.2:35414588-35414607 MS.gene95478:CDS 55.0%
AGGAACCGAAACCCACCGAT+GGG + chr5.2:35413537-35413556 MS.gene95478:CDS 55.0%
AGGTGGGCCAACGATAGTGA+CGG - chr5.2:35413376-35413395 None:intergenic 55.0%
ATCGAGACAAGGGTTACCGG+TGG - chr5.2:35413595-35413614 None:intergenic 55.0%
CTCTCGACGAGTGTCTCAGA+GGG - chr5.2:35413645-35413664 None:intergenic 55.0%
CTGATACTCAGGGCCAGCAA+TGG - chr5.2:35415311-35415330 None:intergenic 55.0%
CTTTCTGAGTAGCCGGCACT+GGG - chr5.2:35415195-35415214 None:intergenic 55.0%
CTTTGTGCTCCCTGTGGTTG+TGG - chr5.2:35414010-35414029 None:intergenic 55.0%
GAGAAGTGGCTGAGTTGCAG+TGG + chr5.2:35414681-35414700 MS.gene95478:CDS 55.0%
GAGTATGGATGGGACCCCAA+TGG + chr5.2:35414775-35414794 MS.gene95478:CDS 55.0%
GCAGCTTCCATGGCTTCTTC+AGG - chr5.2:35415289-35415308 None:intergenic 55.0%
GCCACTCCTTTCTGAGTAGC+CGG - chr5.2:35415202-35415221 None:intergenic 55.0%
GCCTTCTTTGTCGGACTTGC+CGG - chr5.2:35413742-35413761 None:intergenic 55.0%
GGTCGCCAACTTCAACTCTC+TGG + chr5.2:35413512-35413531 MS.gene95478:CDS 55.0%
TACTCAGAAAGGAGTGGCGC+TGG + chr5.2:35415204-35415223 MS.gene95478:CDS 55.0%
TATGGATGGGACCCCAATGG+AGG + chr5.2:35414778-35414797 MS.gene95478:CDS 55.0%
TCTCTGTCAACCCCATCGGT+GGG - chr5.2:35413551-35413570 None:intergenic 55.0%
TGCCACAGACACCTCCATTG+GGG - chr5.2:35414792-35414811 None:intergenic 55.0%
TGCGAAGAGGATCTTCCCTC+GGG + chr5.2:35414346-35414365 MS.gene95478:CDS 55.0%
TGTAACCTCCGTGGTGTTCG+CGG + chr5.2:35413714-35413733 MS.gene95478:CDS 55.0%
TGTTGGCGGTGGTAGGGTTA+GGG - chr5.2:35413422-35413441 None:intergenic 55.0%
TTCTCTGTCAACCCCATCGG+TGG - chr5.2:35413552-35413571 None:intergenic 55.0%
TTTCTGAGTAGCCGGCACTG+GGG - chr5.2:35415194-35415213 None:intergenic 55.0%
! AGTGCCGTTGAGGAAGGTGT+TGG + chr5.2:35414439-35414458 MS.gene95478:CDS 55.0%
! CACAACCACAGGGAGCACAA+AGG + chr5.2:35414008-35414027 MS.gene95478:CDS 55.0%
! CTTGGTAATCGGTCTCCCAG+GGG - chr5.2:35415087-35415106 None:intergenic 55.0%
! GCTTGGTAATCGGTCTCCCA+GGG - chr5.2:35415088-35415107 None:intergenic 55.0%
! TCAACCCCATCGGTGGGTTT+CGG - chr5.2:35413545-35413564 None:intergenic 55.0%
! TGCTTGGTAATCGGTCTCCC+AGG - chr5.2:35415089-35415108 None:intergenic 55.0%
!! AGTAGTTGTGGTGGTGGGGA+CGG - chr5.2:35413469-35413488 None:intergenic 55.0%
!! GAAGGTGTTGGAGTTGCCTG+AGG + chr5.2:35414451-35414470 MS.gene95478:CDS 55.0%
!! GTGAGGGATATGGATTGGGG+AGG + chr5.2:35414932-35414951 MS.gene95478:CDS 55.0%
ACGCACCTTTGTGCTCCCTG+TGG - chr5.2:35414016-35414035 None:intergenic 60.0%
ATGGCCGCCGTCACTATCGT+TGG + chr5.2:35413366-35413385 MS.gene95478:CDS 60.0%
CCTCTCGACGAGTGTCTCAG+AGG - chr5.2:35413646-35413665 None:intergenic 60.0%
CCTTTCTGAGTAGCCGGCAC+TGG - chr5.2:35415196-35415215 None:intergenic 60.0%
GAAGAGGATCTTCCCTCGGG+AGG + chr5.2:35414349-35414368 MS.gene95478:CDS 60.0%
GCCAGCAATGGCAGCTTCCA+TGG - chr5.2:35415299-35415318 None:intergenic 60.0%
GCCATGGAAGCTGCCATTGC+TGG + chr5.2:35415295-35415314 MS.gene95478:CDS 60.0%
GCCGGCTACTCAGAAAGGAG+TGG + chr5.2:35415198-35415217 MS.gene95478:CDS 60.0%
GCTTCTACGCCGCTGCTCTA+TGG + chr5.2:35413760-35413779 MS.gene95478:CDS 60.0%
GGAACCGAAACCCACCGATG+GGG + chr5.2:35413538-35413557 MS.gene95478:CDS 60.0%
TGGGCCAACGATAGTGACGG+CGG - chr5.2:35413373-35413392 None:intergenic 60.0%
! ACAGGGAGCACAAAGGTGCG+TGG + chr5.2:35414015-35414034 MS.gene95478:CDS 60.0%
! GACTGAAGAGGTGGCGGCAA+GGG + chr5.2:35414064-35414083 MS.gene95478:CDS 60.0%
! GTGTTGGCGGTGGTAGGGTT+AGG - chr5.2:35413423-35413442 None:intergenic 60.0%
! TGACTGAAGAGGTGGCGGCA+AGG + chr5.2:35414063-35414082 MS.gene95478:CDS 60.0%
!! AAAGGAGTGGCGCTGGTGAG+TGG + chr5.2:35415211-35415230 MS.gene95478:CDS 60.0%
!! CCAGTGCCGGCTACTCAGAA+AGG + chr5.2:35415193-35415212 MS.gene95478:CDS 60.0%
!! CCTCTGAGACACTCGTCGAG+AGG + chr5.2:35413643-35413662 MS.gene95478:CDS 60.0%
!! CGATGGTGTTGGCGGTGGTA+GGG - chr5.2:35413428-35413447 None:intergenic 60.0%
!! GACCCCAATGGAGGTGTCTG+TGG + chr5.2:35414787-35414806 MS.gene95478:CDS 60.0%
!! TTGAGCGATGGTGTTGGCGG+TGG - chr5.2:35413433-35413452 None:intergenic 60.0%
CTCCGTGGTGTTCGCGGTAC+CGG + chr5.2:35413720-35413739 MS.gene95478:CDS 65.0%
TGCCGGTACCGCGAACACCA+CGG - chr5.2:35413725-35413744 None:intergenic 65.0%
! AGAGGTGGCGGCAAGGGCTA+AGG + chr5.2:35414070-35414089 MS.gene95478:CDS 65.0%
!! AGTTGTGGTGGTGGGGACGG+TGG - chr5.2:35413466-35413485 None:intergenic 65.0%
!! GCGATGGTGTTGGCGGTGGT+AGG - chr5.2:35413429-35413448 None:intergenic 65.0%
CGGTACCGCGAACACCACGG+AGG - chr5.2:35413722-35413741 None:intergenic 70.0%
CTCAAGGGCCACCCCAGTGC+CGG + chr5.2:35415180-35415199 MS.gene95478:CDS 70.0%
! CTGAGTAGCCGGCACTGGGG+TGG - chr5.2:35415191-35415210 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 35413366 35415351 35413366 ID=MS.gene95478
chr5.2 mRNA 35413366 35415351 35413366 ID=MS.gene95478.t1;Parent=MS.gene95478
chr5.2 exon 35413366 35415351 35413366 ID=MS.gene95478.t1.exon1;Parent=MS.gene95478.t1
chr5.2 CDS 35413366 35415351 35413366 ID=cds.MS.gene95478.t1;Parent=MS.gene95478.t1
Gene Sequence

>MS.gene95478

ATGGCCGCCGTCACTATCGTTGGCCCACCTGAGATCTACTCTCTCAATTCAAACCCTAACCCTACCACCGCCAACACCATCGCTCAAAAAACTGAAACCACCGTCCCCACCACCACAACTACTAACGATGTCTTCGTCGATCAAATGGTCGCCAACTTCAACTCTCTGGGAAGGAACCGAAACCCACCGATGGGGTTGACAGAGAACATGTCACCGACATTTCTCTCCACCGGTAACCCTTGTCTCGATTTCTTCTTCCATGTTGTTCCTGATACTCCCTCTGAGACACTCGTCGAGAGGCTCAAACTAGCTTGGTCACACAACCCACTCACCACTCTCAAACTCGTCTGTAACCTCCGTGGTGTTCGCGGTACCGGCAAGTCCGACAAAGAAGGCTTCTACGCCGCTGCTCTATGGTTTCATGAAAATCACCCTAAAACCCTAGCTACCAATATTCCTTCCCTCGCTGACTTTGGTTACTTCAAAGATCTCCCTGAAATTCTATACCGTATTCTCGAAGGTTCTGGAATTCGTGAAATCCAGAAAGAAGAGTGGAATGAAAGGAAGTCTGGATCCAAAAATAAGCGCTTGCAACAATCAAACCCTTTTGCTTTAAGAAGAAGAATAAATAAAAAACAACGCCACAACCACAGGGAGCACAAAGGTGCGTGGAAACTCACTGACAAAGACTCTTTGATGACTGAAGAGGTGGCGGCAAGGGCTAAGGCTCAGAAACAAACCGCTCACGTTTTGAAGGAAGAGAAGAGGATTGCATTGGCTAAGAAGCTTGTTGATCGTTACACTAACGATCCCAATTTTAAGTTCCTTCATGATTCTATCTCTGATCATTTTGCTGATTGTTTGAAGAAGGATCTCGAGTTTTTGAAATCTGGGTCACCCACAAAAATCAGTCTTGCTGCTAAATGGTGCCCTTCTGTTGACTCTTCCTTTGATCGGTCAACTCTTCTTTGTGAGACAATTGCGAAGAGGATCTTCCCTCGGGAGGAATATCAAGGTGTGGAGGAGGCGCATTATGCTTACAGGGTTCGTGATCGTTTGAGGAAAGATGTGTTAGTGCCGTTGAGGAAGGTGTTGGAGTTGCCTGAGGTGTTTATTGGTGCTAATCAATGGGGTTTGATTCCTTACAACAGGGTTGCTTCTGTGGCCATGAAGTTTTATAAGGAGAAGTTTTTGAAGCATGATAAGGAGAGGTTTGAGAAGTACTTGGAGGATGTGAAGGCGGGGAAGACTACTATTGCTGCTGGTGCTTTGCTTCCTCATGAGATTATAGAGTCTTTGGGTGATGGAGATGGTGGAGAAGTGGCTGAGTTGCAGTGGAAGAGAATCGTTGATGATTTGCTCAAGAAGGGAAAGATGAAAAATTGTCTTGCTGTGTGTGATGTGTCTGGGAGTATGGATGGGACCCCAATGGAGGTGTCTGTGGCATTGGGATTGTTGGTGTCTGAATTGAGTGATGAACCTTGGAAAGGGAAAGTTATTACTTTCAGCGAGGAGCCTCAGCTTCATGTGATTAAGGGGGATAATCTCAAGTCCAAGACACAGTTTGTGAGGGATATGGATTGGGGAGGGAACACTGATTTCCAGAAGGTGTTTGATCGCATATTAGAGGTGGCTGTTAATGGGAATCTCAAGGAGGATCAGATGATTAAAAGGATCTTCGTATTCAGTGACATGGAGTTTGATCAAGCGTCTATGAACCCCTGGGAGACCGATTACCAAGCAATCACTAGGAAGTATAGCGAGAAAGGGTATGGTTCTGTTGTGCCTCAGATAGTTTTTTGGAATCTGAGAGACTCAAGGGCCACCCCAGTGCCGGCTACTCAGAAAGGAGTGGCGCTGGTGAGTGGTTTCTCTAAGAATCTGCTGACACTGTTCTTAGATAATGAAGGTGAAATAAGCCCTGAAGAAGCCATGGAAGCTGCCATTGCTGGCCCTGAGTATCAGAAACTAGTTGTGTTGGATTGA
Protein sequence

>MS.gene95478.t1

MAAVTIVGPPEIYSLNSNPNPTTANTIAQKTETTVPTTTTTNDVFVDQMVANFNSLGRNRNPPMGLTENMSPTFLSTGNPCLDFFFHVVPDTPSETLVERLKLAWSHNPLTTLKLVCNLRGVRGTGKSDKEGFYAAALWFHENHPKTLATNIPSLADFGYFKDLPEILYRILEGSGIREIQKEEWNERKSGSKNKRLQQSNPFALRRRINKKQRHNHREHKGAWKLTDKDSLMTEEVAARAKAQKQTAHVLKEEKRIALAKKLVDRYTNDPNFKFLHDSISDHFADCLKKDLEFLKSGSPTKISLAAKWCPSVDSSFDRSTLLCETIAKRIFPREEYQGVEEAHYAYRVRDRLRKDVLVPLRKVLELPEVFIGANQWGLIPYNRVASVAMKFYKEKFLKHDKERFEKYLEDVKAGKTTIAAGALLPHEIIESLGDGDGGEVAELQWKRIVDDLLKKGKMKNCLAVCDVSGSMDGTPMEVSVALGLLVSELSDEPWKGKVITFSEEPQLHVIKGDNLKSKTQFVRDMDWGGNTDFQKVFDRILEVAVNGNLKEDQMIKRIFVFSDMEFDQASMNPWETDYQAITRKYSEKGYGSVVPQIVFWNLRDSRATPVPATQKGVALVSGFSKNLLTLFLDNEGEISPEEAMEAAIAGPEYQKLVVLD