Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62884.t1 | PNX79454.1 | 96.6 | 118 | 4 | 0 | 1 | 118 | 14 | 131 | 2.00E-44 | 188.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62884.t1 | P14009 | 65.5 | 84 | 29 | 0 | 35 | 118 | 54 | 137 | 1.9e-24 | 113.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62884.t1 | A0A2K3LLP0 | 96.6 | 118 | 4 | 0 | 1 | 118 | 14 | 131 | 1.4e-44 | 188.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene62884 | -0.829937 | 3.89E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene62884 | -0.838963 | 2.10E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene62884 | 0.817992 | 2.47E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene62884 | -0.800484 | 1.41E-48 | -1.69E-46 |
| MS.gene04932 | MS.gene62884 | 0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene049633 | MS.gene62884 | -0.828799 | 7.35E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene62884 | -0.8248 | 6.63E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene62884 | -0.812216 | 4.74E-51 | -1.69E-46 |
| MS.gene050338 | MS.gene62884 | 0.849921 | 2.37E-60 | -1.69E-46 |
| MS.gene050444 | MS.gene62884 | -0.820911 | 5.34E-53 | -1.69E-46 |
| MS.gene050504 | MS.gene62884 | -0.817621 | 3.00E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene62884 | -0.862409 | 5.17E-64 | -1.69E-46 |
| MS.gene050612 | MS.gene62884 | -0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene050710 | MS.gene62884 | 0.821376 | 4.17E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene62884 | -0.81817 | 2.25E-52 | -1.69E-46 |
| MS.gene05080 | MS.gene62884 | -0.827572 | 1.45E-54 | -1.69E-46 |
| MS.gene05081 | MS.gene62884 | -0.820992 | 5.12E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene62884 | -0.838826 | 2.28E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene62884 | -0.838664 | 2.51E-57 | -1.69E-46 |
| MS.gene050875 | MS.gene62884 | 0.803367 | 3.61E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene62884 | -0.833403 | 5.44E-56 | -1.69E-46 |
| MS.gene050950 | MS.gene62884 | -0.817031 | 4.07E-52 | -1.69E-46 |
| MS.gene051138 | MS.gene62884 | 0.820855 | 5.50E-53 | -1.69E-46 |
| MS.gene051317 | MS.gene62884 | -0.825343 | 4.94E-54 | -1.69E-46 |
| MS.gene051439 | MS.gene62884 | 0.832841 | 7.51E-56 | -1.69E-46 |
| MS.gene051651 | MS.gene62884 | 0.819037 | 1.43E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene62884 | -0.849482 | 3.14E-60 | -1.69E-46 |
| MS.gene051941 | MS.gene62884 | -0.804371 | 2.23E-49 | -1.69E-46 |
| MS.gene052100 | MS.gene62884 | -0.812692 | 3.73E-51 | -1.69E-46 |
| MS.gene052129 | MS.gene62884 | 0.801263 | 9.78E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene62884 | -0.840557 | 8.09E-58 | -1.69E-46 |
| MS.gene05240 | MS.gene62884 | 0.821684 | 3.54E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene62884 | -0.819376 | 1.20E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene62884 | -0.818399 | 2.00E-52 | -1.69E-46 |
| MS.gene052494 | MS.gene62884 | 0.800873 | 1.17E-48 | -1.69E-46 |
| MS.gene052503 | MS.gene62884 | -0.824325 | 8.58E-54 | -1.69E-46 |
| MS.gene052606 | MS.gene62884 | 0.825763 | 3.93E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene62884 | -0.827373 | 1.62E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene62884 | -0.825059 | 5.76E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene62884 | -0.803481 | 3.41E-49 | -1.69E-46 |
| MS.gene053108 | MS.gene62884 | 0.818587 | 1.81E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene62884 | -0.833469 | 5.24E-56 | -1.69E-46 |
| MS.gene053420 | MS.gene62884 | 0.803035 | 4.23E-49 | -1.69E-46 |
| MS.gene053421 | MS.gene62884 | 0.820189 | 7.82E-53 | -1.69E-46 |
| MS.gene053479 | MS.gene62884 | -0.82536 | 4.89E-54 | -1.69E-46 |
| MS.gene053480 | MS.gene62884 | -0.829499 | 4.98E-55 | -1.69E-46 |
| MS.gene053632 | MS.gene62884 | -0.804805 | 1.81E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene62884 | -0.821043 | 4.98E-53 | -1.69E-46 |
| MS.gene053920 | MS.gene62884 | 0.80233 | 5.91E-49 | -1.69E-46 |
| MS.gene054344 | MS.gene62884 | -0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene62884 | -0.817422 | 3.33E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene62884 | 0.828956 | 6.74E-55 | -1.69E-46 |
| MS.gene05470 | MS.gene62884 | -0.860719 | 1.70E-63 | -1.69E-46 |
| MS.gene054742 | MS.gene62884 | 0.830112 | 3.53E-55 | -1.69E-46 |
| MS.gene054884 | MS.gene62884 | 0.82999 | 3.78E-55 | -1.69E-46 |
| MS.gene054941 | MS.gene62884 | 0.824511 | 7.76E-54 | -1.69E-46 |
| MS.gene054965 | MS.gene62884 | 0.801096 | 1.06E-48 | -1.69E-46 |
| MS.gene055158 | MS.gene62884 | 0.826036 | 3.38E-54 | -1.69E-46 |
| MS.gene055206 | MS.gene62884 | -0.816279 | 6.00E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene62884 | -0.832853 | 7.46E-56 | -1.69E-46 |
| MS.gene055577 | MS.gene62884 | -0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene055729 | MS.gene62884 | 0.806829 | 6.80E-50 | -1.69E-46 |
| MS.gene055730 | MS.gene62884 | 0.802572 | 5.27E-49 | -1.69E-46 |
| MS.gene055731 | MS.gene62884 | 0.809271 | 2.06E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene62884 | -0.852334 | 4.94E-61 | -1.69E-46 |
| MS.gene055750 | MS.gene62884 | -0.83372 | 4.54E-56 | -1.69E-46 |
| MS.gene055751 | MS.gene62884 | -0.84685 | 1.68E-59 | -1.69E-46 |
| MS.gene05589 | MS.gene62884 | -0.865326 | 6.40E-65 | -1.69E-46 |
| MS.gene055941 | MS.gene62884 | -0.812999 | 3.20E-51 | -1.69E-46 |
| MS.gene056283 | MS.gene62884 | -0.802445 | 5.59E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene62884 | -0.848561 | 5.67E-60 | -1.69E-46 |
| MS.gene056504 | MS.gene62884 | -0.83665 | 8.26E-57 | -1.69E-46 |
| MS.gene056583 | MS.gene62884 | 0.802446 | 5.59E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene62884 | 0.824382 | 8.32E-54 | -1.69E-46 |
| MS.gene056591 | MS.gene62884 | 0.82326 | 1.52E-53 | -1.69E-46 |
| MS.gene056618 | MS.gene62884 | 0.826742 | 2.30E-54 | -1.69E-46 |
| MS.gene056678 | MS.gene62884 | 0.809432 | 1.90E-50 | -1.69E-46 |
| MS.gene056688 | MS.gene62884 | 0.823933 | 1.06E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene62884 | -0.832337 | 1.00E-55 | -1.69E-46 |
| MS.gene056694 | MS.gene62884 | 0.833998 | 3.86E-56 | -1.69E-46 |
| MS.gene056697 | MS.gene62884 | 0.851978 | 6.24E-61 | -1.69E-46 |
| MS.gene056748 | MS.gene62884 | 0.801448 | 8.96E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene62884 | 0.828585 | 8.28E-55 | -1.69E-46 |
| MS.gene057261 | MS.gene62884 | 0.80796 | 3.92E-50 | -1.69E-46 |
| MS.gene057332 | MS.gene62884 | -0.818146 | 2.28E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene62884 | -0.8485 | 5.89E-60 | -1.69E-46 |
| MS.gene057717 | MS.gene62884 | 0.825024 | 5.87E-54 | -1.69E-46 |
| MS.gene057768 | MS.gene62884 | 0.810003 | 1.43E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene62884 | -0.816505 | 5.34E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene62884 | -0.805594 | 1.24E-49 | -1.69E-46 |
| MS.gene057857 | MS.gene62884 | -0.814185 | 1.75E-51 | -1.69E-46 |
| MS.gene05787 | MS.gene62884 | -0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene058171 | MS.gene62884 | 0.800809 | 1.21E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene62884 | 0.806528 | 7.87E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene62884 | 0.814324 | 1.63E-51 | -1.69E-46 |
| MS.gene058338 | MS.gene62884 | -0.812074 | 5.09E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene62884 | -0.818923 | 1.52E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene62884 | 0.812846 | 3.45E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene62884 | -0.813775 | 2.16E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene62884 | -0.827891 | 1.22E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene62884 | -0.841404 | 4.85E-58 | -1.69E-46 |
| MS.gene059023 | MS.gene62884 | -0.835151 | 1.98E-56 | -1.69E-46 |
| MS.gene05914 | MS.gene62884 | -0.836703 | 8.01E-57 | -1.69E-46 |
| MS.gene05926 | MS.gene62884 | 0.856915 | 2.33E-62 | -1.69E-46 |
| MS.gene05934 | MS.gene62884 | -0.817617 | 3.01E-52 | -1.69E-46 |
| MS.gene05960 | MS.gene62884 | 0.845254 | 4.56E-59 | -1.69E-46 |
| MS.gene059667 | MS.gene62884 | -0.844986 | 5.38E-59 | -1.69E-46 |
| MS.gene059677 | MS.gene62884 | -0.800026 | 1.75E-48 | -1.69E-46 |
| MS.gene059718 | MS.gene62884 | -0.803285 | 3.75E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene62884 | -0.818098 | 2.34E-52 | -1.69E-46 |
| MS.gene06000 | MS.gene62884 | -0.863259 | 2.83E-64 | -1.69E-46 |
| MS.gene060087 | MS.gene62884 | 0.817449 | 3.28E-52 | -1.69E-46 |
| MS.gene060405 | MS.gene62884 | 0.828707 | 7.74E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene62884 | -0.842839 | 2.02E-58 | -1.69E-46 |
| MS.gene060778 | MS.gene62884 | -0.800781 | 1.23E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene62884 | -0.824178 | 9.29E-54 | -1.69E-46 |
| MS.gene06080 | MS.gene62884 | 0.800056 | 1.72E-48 | -1.69E-46 |
| MS.gene060823 | MS.gene62884 | 0.807251 | 5.54E-50 | -1.69E-46 |
| MS.gene06084 | MS.gene62884 | 0.803122 | 4.05E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene62884 | -0.833514 | 5.11E-56 | -1.69E-46 |
| MS.gene061044 | MS.gene62884 | 0.819881 | 9.20E-53 | -1.69E-46 |
| MS.gene061231 | MS.gene62884 | -0.802876 | 4.56E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62884.t1 | MTR_4g101310 | 96.610 | 118 | 4 | 0 | 1 | 118 | 17 | 134 | 3.59e-78 | 226 |
| MS.gene62884.t1 | MTR_4g101330 | 50.000 | 152 | 41 | 2 | 1 | 117 | 17 | 168 | 1.18e-39 | 130 |
| MS.gene62884.t1 | MTR_4g101280 | 67.045 | 88 | 29 | 0 | 31 | 118 | 56 | 143 | 8.13e-38 | 125 |
| MS.gene62884.t1 | MTR_1g012710 | 61.628 | 86 | 32 | 1 | 33 | 118 | 67 | 151 | 1.55e-34 | 117 |
| MS.gene62884.t1 | MTR_1g012690 | 56.190 | 105 | 37 | 3 | 14 | 118 | 59 | 154 | 1.60e-34 | 117 |
| MS.gene62884.t1 | MTR_1g012700 | 50.442 | 113 | 47 | 2 | 14 | 118 | 53 | 164 | 1.92e-32 | 112 |
| MS.gene62884.t1 | MTR_4g108150 | 48.739 | 119 | 52 | 3 | 1 | 118 | 13 | 123 | 1.93e-31 | 108 |
| MS.gene62884.t1 | MTR_2g026760 | 52.941 | 119 | 49 | 5 | 1 | 118 | 15 | 127 | 2.29e-31 | 108 |
| MS.gene62884.t1 | MTR_3g106740 | 62.353 | 85 | 30 | 2 | 34 | 117 | 46 | 129 | 3.35e-31 | 107 |
| MS.gene62884.t1 | MTR_1g012630 | 58.140 | 86 | 35 | 1 | 33 | 118 | 126 | 210 | 6.99e-31 | 109 |
| MS.gene62884.t1 | MTR_4g101260 | 49.587 | 121 | 57 | 3 | 1 | 118 | 13 | 132 | 6.44e-30 | 104 |
| MS.gene62884.t1 | MTR_5g012340 | 50.575 | 87 | 42 | 1 | 31 | 117 | 91 | 176 | 4.91e-26 | 96.3 |
| MS.gene62884.t1 | MTR_5g012380 | 50.575 | 87 | 42 | 1 | 31 | 117 | 84 | 169 | 6.80e-26 | 95.5 |
| MS.gene62884.t1 | MTR_3g106750 | 54.762 | 84 | 38 | 0 | 35 | 118 | 46 | 129 | 2.32e-25 | 92.8 |
| MS.gene62884.t1 | MTR_4g101270 | 51.765 | 85 | 40 | 1 | 33 | 117 | 103 | 186 | 3.25e-24 | 91.7 |
| MS.gene62884.t1 | MTR_2g026920 | 44.444 | 81 | 42 | 1 | 38 | 118 | 118 | 195 | 6.28e-17 | 72.8 |
| MS.gene62884.t1 | MTR_2g026930 | 44.444 | 81 | 42 | 1 | 38 | 118 | 122 | 199 | 8.43e-17 | 72.8 |
| MS.gene62884.t1 | MTR_3g082100 | 45.977 | 87 | 46 | 1 | 31 | 117 | 250 | 335 | 5.08e-16 | 72.4 |
| MS.gene62884.t1 | MTR_2g026775 | 48.101 | 79 | 35 | 2 | 42 | 118 | 686 | 760 | 4.34e-14 | 67.4 |
| MS.gene62884.t1 | MTR_2g026820 | 49.367 | 79 | 34 | 3 | 40 | 117 | 542 | 615 | 9.69e-14 | 66.2 |
| MS.gene62884.t1 | MTR_4g115360 | 44.444 | 90 | 49 | 1 | 29 | 118 | 145 | 233 | 1.40e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62884.t1 | AT1G12100 | 55.932 | 118 | 50 | 1 | 1 | 118 | 17 | 132 | 7.54e-39 | 127 |
| MS.gene62884.t1 | AT1G62510 | 66.667 | 84 | 28 | 0 | 34 | 117 | 66 | 149 | 5.10e-36 | 120 |
| MS.gene62884.t1 | AT4G12500 | 57.955 | 88 | 37 | 0 | 31 | 118 | 90 | 177 | 1.50e-34 | 117 |
| MS.gene62884.t1 | AT4G12520 | 50.000 | 118 | 54 | 1 | 1 | 118 | 17 | 129 | 3.40e-34 | 115 |
| MS.gene62884.t1 | AT4G12510 | 50.000 | 118 | 54 | 1 | 1 | 118 | 17 | 129 | 3.40e-34 | 115 |
| MS.gene62884.t1 | AT4G12490 | 56.989 | 93 | 40 | 0 | 26 | 118 | 90 | 182 | 9.28e-34 | 115 |
| MS.gene62884.t1 | AT2G45180 | 63.529 | 85 | 30 | 1 | 34 | 118 | 51 | 134 | 2.38e-33 | 113 |
| MS.gene62884.t1 | AT4G22460 | 53.571 | 112 | 51 | 1 | 5 | 116 | 21 | 131 | 2.91e-33 | 113 |
| MS.gene62884.t1 | AT1G12090 | 65.432 | 81 | 28 | 0 | 37 | 117 | 57 | 137 | 1.64e-32 | 111 |
| MS.gene62884.t1 | AT4G12480 | 55.682 | 88 | 39 | 0 | 31 | 118 | 81 | 168 | 2.03e-32 | 112 |
| MS.gene62884.t1 | AT4G12470 | 55.056 | 89 | 40 | 0 | 30 | 118 | 73 | 161 | 3.59e-32 | 111 |
| MS.gene62884.t1 | AT4G12550 | 49.495 | 99 | 48 | 2 | 21 | 118 | 14 | 111 | 2.54e-29 | 102 |
| MS.gene62884.t1 | AT5G46890 | 47.458 | 118 | 58 | 2 | 1 | 118 | 14 | 127 | 6.86e-28 | 99.4 |
| MS.gene62884.t1 | AT4G00165 | 48.544 | 103 | 51 | 2 | 16 | 117 | 27 | 128 | 1.37e-27 | 98.6 |
| MS.gene62884.t1 | AT4G00165 | 48.544 | 103 | 51 | 2 | 16 | 117 | 27 | 128 | 1.37e-27 | 98.6 |
| MS.gene62884.t1 | AT4G12530 | 54.945 | 91 | 40 | 1 | 27 | 117 | 26 | 115 | 1.03e-26 | 95.9 |
| MS.gene62884.t1 | AT5G46900 | 54.444 | 90 | 38 | 1 | 29 | 118 | 41 | 127 | 1.03e-26 | 96.3 |
| MS.gene62884.t1 | AT4G12545 | 49.495 | 99 | 45 | 3 | 21 | 118 | 14 | 108 | 6.29e-26 | 94.0 |
| MS.gene62884.t1 | AT3G22120 | 50.000 | 86 | 41 | 2 | 33 | 117 | 265 | 349 | 2.22e-19 | 81.6 |
| MS.gene62884.t1 | AT3G22120 | 50.000 | 86 | 41 | 2 | 33 | 117 | 248 | 332 | 3.34e-19 | 81.3 |
| MS.gene62884.t1 | AT3G22142 | 50.538 | 93 | 43 | 2 | 27 | 117 | 1387 | 1478 | 3.23e-18 | 79.3 |
| MS.gene62884.t1 | AT1G62500 | 46.429 | 84 | 44 | 1 | 34 | 117 | 211 | 293 | 9.61e-18 | 76.6 |
| MS.gene62884.t1 | AT2G10940 | 44.706 | 85 | 46 | 1 | 34 | 118 | 207 | 290 | 1.29e-17 | 76.3 |
| MS.gene62884.t1 | AT2G10940 | 44.706 | 85 | 46 | 1 | 34 | 118 | 207 | 290 | 1.29e-17 | 76.3 |
| MS.gene62884.t1 | AT4G15160 | 44.186 | 86 | 47 | 1 | 33 | 117 | 97 | 182 | 1.05e-16 | 72.4 |
| MS.gene62884.t1 | AT4G15160 | 44.186 | 86 | 47 | 1 | 33 | 117 | 179 | 264 | 3.10e-16 | 72.4 |
| MS.gene62884.t1 | AT4G22490 | 35.870 | 92 | 56 | 2 | 26 | 117 | 28 | 116 | 1.10e-11 | 57.8 |
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGATCAAACTTAGTGAAAT+AGG | 0.251033 | 8.3:+19984808 | None:intergenic |
| TTTGGATTAGGGTTTGGTTT+TGG | 0.251880 | 8.3:+19985057 | None:intergenic |
| GTGTGTGCTAATTTGCTTAA+TGG | 0.265220 | 8.3:-19984967 | MS.gene62884:CDS |
| AAGGGAATGGATTTGGATTA+GGG | 0.269618 | 8.3:+19985046 | None:intergenic |
| GCCAAGAATGTTAGCTTTGA+TGG | 0.271570 | 8.3:+19984846 | None:intergenic |
| CTTGGGTAAGGGAATGGATT+TGG | 0.286724 | 8.3:+19985039 | None:intergenic |
| TCCAAGAGATGCACTCAAAT+TGG | 0.300623 | 8.3:-19984992 | MS.gene62884:CDS |
| TAAGGGAATGGATTTGGATT+AGG | 0.323838 | 8.3:+19985045 | None:intergenic |
| GCCATCAAAGCTAACATTCT+TGG | 0.340537 | 8.3:-19984847 | MS.gene62884:CDS |
| ATAGAGCAACATGGATGTTC+TGG | 0.348478 | 8.3:+19984916 | None:intergenic |
| CCCAATTTGAGTGCATCTCT+TGG | 0.362878 | 8.3:+19984991 | None:intergenic |
| GAGTCTTCTCACAAGCATTA+AGG | 0.369143 | 8.3:+19984788 | None:intergenic |
| AATGGATTTGGATTAGGGTT+TGG | 0.380389 | 8.3:+19985051 | None:intergenic |
| GAAGGACTTGTGCTTGGGTA+AGG | 0.431525 | 8.3:+19985027 | None:intergenic |
| GGACCAATTGGTGCAGTGAT+TGG | 0.437596 | 8.3:-19984946 | MS.gene62884:CDS |
| CCAAGAGATGCACTCAAATT+GGG | 0.438012 | 8.3:-19984991 | MS.gene62884:CDS |
| GACCCAATCACTGCACCAAT+TGG | 0.459457 | 8.3:+19984943 | None:intergenic |
| TAGTGGAAGGACTTGTGCTT+GGG | 0.461750 | 8.3:+19985022 | None:intergenic |
| AATTTGCTTAATGGACCAAT+TGG | 0.477291 | 8.3:-19984958 | MS.gene62884:CDS |
| ATCTCTTGGACAACTCTTAG+TGG | 0.495008 | 8.3:+19985005 | None:intergenic |
| TTAGTGGAAGGACTTGTGCT+TGG | 0.502701 | 8.3:+19985021 | None:intergenic |
| CTTGTGCTTGGGTAAGGGAA+TGG | 0.504980 | 8.3:+19985033 | None:intergenic |
| GACCAATTGGTGCAGTGATT+GGG | 0.509536 | 8.3:-19984945 | MS.gene62884:CDS |
| TAACACTTAATTAAGAGCAC+TGG | 0.528966 | 8.3:+19984752 | None:intergenic |
| AAGGACTTGTGCTTGGGTAA+GGG | 0.591360 | 8.3:+19985028 | None:intergenic |
| TAAGAGCACTGGAAATCAGT+GGG | 0.619022 | 8.3:+19984763 | None:intergenic |
| CTTGGACAACTCTTAGTGGA+AGG | 0.619262 | 8.3:+19985009 | None:intergenic |
| CCTTCTAAAATAGAGCAACA+TGG | 0.620844 | 8.3:+19984907 | None:intergenic |
| TTAAGAGCACTGGAAATCAG+TGG | 0.653235 | 8.3:+19984762 | None:intergenic |
| GAGCACTGGAAATCAGTGGG+TGG | 0.693320 | 8.3:+19984766 | None:intergenic |
| GAGCAACATGGATGTTCTGG+TGG | 0.695269 | 8.3:+19984919 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGATCAAACTTAGTGAAAT+AGG | + | chr8.3:19985053-19985072 | None:intergenic | 30.0% | |
| ! | AATTTGCTTAATGGACCAAT+TGG | - | chr8.3:19984900-19984919 | MS.gene62884:CDS | 30.0% |
| ! | CTTTTCTTTACAATGGCAAA+TGG | - | chr8.3:19984765-19984784 | MS.gene62884:CDS | 30.0% |
| ! | AAGGGAATGGATTTGGATTA+GGG | + | chr8.3:19984815-19984834 | None:intergenic | 35.0% |
| ! | AATGGATTTGGATTAGGGTT+TGG | + | chr8.3:19984810-19984829 | None:intergenic | 35.0% |
| ! | TAAGGGAATGGATTTGGATT+AGG | + | chr8.3:19984816-19984835 | None:intergenic | 35.0% |
| !! | CCATGTTGCTCTATTTTAGA+AGG | - | chr8.3:19984951-19984970 | MS.gene62884:CDS | 35.0% |
| !! | CCTTCTAAAATAGAGCAACA+TGG | + | chr8.3:19984954-19984973 | None:intergenic | 35.0% |
| !! | GTGTGTGCTAATTTGCTTAA+TGG | - | chr8.3:19984891-19984910 | MS.gene62884:CDS | 35.0% |
| !! | TTTGGATTAGGGTTTGGTTT+TGG | + | chr8.3:19984804-19984823 | None:intergenic | 35.0% |
| ATAGAGCAACATGGATGTTC+TGG | + | chr8.3:19984945-19984964 | None:intergenic | 40.0% | |
| ATCTCTTGGACAACTCTTAG+TGG | + | chr8.3:19984856-19984875 | None:intergenic | 40.0% | |
| CCAAGAGATGCACTCAAATT+GGG | - | chr8.3:19984867-19984886 | MS.gene62884:CDS | 40.0% | |
| GAGTCTTCTCACAAGCATTA+AGG | + | chr8.3:19985073-19985092 | None:intergenic | 40.0% | |
| GCCATCAAAGCTAACATTCT+TGG | - | chr8.3:19985011-19985030 | MS.gene62884:CDS | 40.0% | |
| TCCAAGAGATGCACTCAAAT+TGG | - | chr8.3:19984866-19984885 | MS.gene62884:CDS | 40.0% | |
| !! | GCCAAGAATGTTAGCTTTGA+TGG | + | chr8.3:19985015-19985034 | None:intergenic | 40.0% |
| !! | TAAGAGCACTGGAAATCAGT+GGG | + | chr8.3:19985098-19985117 | None:intergenic | 40.0% |
| !! | TTAAGAGCACTGGAAATCAG+TGG | + | chr8.3:19985099-19985118 | None:intergenic | 40.0% |
| AAGGACTTGTGCTTGGGTAA+GGG | + | chr8.3:19984833-19984852 | None:intergenic | 45.0% | |
| CCCAATTTGAGTGCATCTCT+TGG | + | chr8.3:19984870-19984889 | None:intergenic | 45.0% | |
| CTTGGACAACTCTTAGTGGA+AGG | + | chr8.3:19984852-19984871 | None:intergenic | 45.0% | |
| GACCAATTGGTGCAGTGATT+GGG | - | chr8.3:19984913-19984932 | MS.gene62884:CDS | 45.0% | |
| TAGTGGAAGGACTTGTGCTT+GGG | + | chr8.3:19984839-19984858 | None:intergenic | 45.0% | |
| TTAGTGGAAGGACTTGTGCT+TGG | + | chr8.3:19984840-19984859 | None:intergenic | 45.0% | |
| ! | CTTGGGTAAGGGAATGGATT+TGG | + | chr8.3:19984822-19984841 | None:intergenic | 45.0% |
| CTTGTGCTTGGGTAAGGGAA+TGG | + | chr8.3:19984828-19984847 | None:intergenic | 50.0% | |
| GAAGGACTTGTGCTTGGGTA+AGG | + | chr8.3:19984834-19984853 | None:intergenic | 50.0% | |
| GACCCAATCACTGCACCAAT+TGG | + | chr8.3:19984918-19984937 | None:intergenic | 50.0% | |
| GAGCAACATGGATGTTCTGG+TGG | + | chr8.3:19984942-19984961 | None:intergenic | 50.0% | |
| GGACCAATTGGTGCAGTGAT+TGG | - | chr8.3:19984912-19984931 | MS.gene62884:CDS | 50.0% | |
| !! | GAGCACTGGAAATCAGTGGG+TGG | + | chr8.3:19985095-19985114 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19984762 | 19985118 | 19984762 | ID=MS.gene62884 |
| chr8.3 | mRNA | 19984762 | 19985118 | 19984762 | ID=MS.gene62884.t1;Parent=MS.gene62884 |
| chr8.3 | exon | 19984762 | 19985118 | 19984762 | ID=MS.gene62884.t1.exon1;Parent=MS.gene62884.t1 |
| chr8.3 | CDS | 19984762 | 19985118 | 19984762 | ID=cds.MS.gene62884.t1;Parent=MS.gene62884.t1 |
| Gene Sequence |
| Protein sequence |
