AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54347


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54347.t1 MTR_7g405740 95.152 165 5 1 1 165 1 162 8.84e-116 325
MS.gene54347.t1 MTR_3g098970 49.612 129 54 4 7 135 1 118 1.31e-31 111
MS.gene54347.t1 MTR_8g096440 41.406 128 59 3 24 144 5 123 2.10e-26 97.8
MS.gene54347.t1 MTR_8g096500 53.165 79 33 1 70 144 49 127 6.47e-24 91.7
MS.gene54347.t1 MTR_8g096470 53.125 64 30 0 71 134 47 110 1.34e-20 82.0
MS.gene54347.t1 MTR_4g072160 53.125 64 30 0 70 133 37 100 1.29e-17 75.1
MS.gene54347.t1 MTR_2g066830 43.478 92 39 1 39 130 40 118 8.78e-17 72.8
MS.gene54347.t1 MTR_3g092220 42.169 83 46 1 56 136 19 101 1.51e-16 71.6
MS.gene54347.t1 MTR_4g124750 45.455 66 36 0 70 135 69 134 1.54e-16 72.8
MS.gene54347.t1 MTR_8g461210 50.769 65 32 0 66 130 31 95 2.80e-16 70.9
MS.gene54347.t1 MTR_3g109160 35.000 140 74 4 15 145 15 146 3.13e-16 72.4
MS.gene54347.t1 MTR_2g043960 40.845 71 42 0 70 140 76 146 3.42e-16 72.0
MS.gene54347.t1 MTR_1g024085 41.026 78 46 0 64 141 31 108 1.03e-15 70.1
MS.gene54347.t1 MTR_7g104950 43.056 72 37 1 73 144 53 120 1.40e-15 69.3
MS.gene54347.t1 MTR_3g117630 42.857 84 42 3 48 129 6 85 2.93e-15 67.8
MS.gene54347.t1 MTR_3g084250 40.476 84 45 2 46 128 5 84 3.16e-15 67.4
MS.gene54347.t1 MTR_1g059770 42.466 73 42 0 62 134 41 113 6.44e-15 67.8
MS.gene54347.t1 MTR_7g118010 44.928 69 36 1 70 136 23 91 1.99e-14 65.9
MS.gene54347.t1 MTR_7g104940 45.161 62 34 0 73 134 53 114 6.67e-14 65.1
MS.gene54347.t1 MTR_4g005320 35.211 71 46 0 69 139 69 139 7.47e-14 65.9
MS.gene54347.t1 MTR_4g072770 41.667 72 41 1 65 135 10 81 1.47e-13 62.8
MS.gene54347.t1 MTR_3g084240 47.541 61 31 1 70 129 25 85 3.74e-13 62.0
MS.gene54347.t1 MTR_1g009150 39.394 66 40 0 70 135 71 136 3.91e-13 64.3
MS.gene54347.t1 MTR_4g072600 38.158 76 46 1 55 129 5 80 4.04e-13 62.0
MS.gene54347.t1 MTR_3g084170 38.824 85 47 2 46 129 5 85 5.20e-13 61.6
MS.gene54347.t1 MTR_4g072220 41.667 72 41 1 56 126 12 83 6.18e-13 61.6
MS.gene54347.t1 MTR_8g026730 43.210 81 40 3 68 142 25 105 6.39e-13 62.0
MS.gene54347.t1 MTR_3g084150 36.471 85 49 2 46 129 5 85 6.43e-13 61.6
MS.gene54347.t1 MTR_3g084180 36.471 85 49 2 46 129 5 85 6.43e-13 61.6
MS.gene54347.t1 MTR_1g061570 37.500 64 40 0 70 133 19 82 1.20e-12 61.6
MS.gene54347.t1 MTR_3g097120 38.824 85 43 2 62 138 2 85 1.44e-12 60.8
MS.gene54347.t1 MTR_4g072300 38.750 80 48 1 56 134 12 91 1.45e-12 60.5
MS.gene54347.t1 MTR_4g072570 40.909 66 38 1 70 134 25 90 1.62e-12 60.5
MS.gene54347.t1 MTR_4g072500 47.541 61 31 1 70 129 25 85 1.82e-12 60.5
MS.gene54347.t1 MTR_7g101740 45.902 61 33 0 70 130 40 100 1.93e-12 60.8
MS.gene54347.t1 MTR_4g050700 49.231 65 30 2 68 129 22 86 2.15e-12 60.1
MS.gene54347.t1 MTR_2g012410 49.231 65 30 2 68 129 22 86 2.15e-12 60.1
MS.gene54347.t1 MTR_8g461410 36.471 85 49 2 46 129 5 85 2.19e-12 60.1
MS.gene54347.t1 MTR_1g094750 44.444 63 35 0 68 130 48 110 2.60e-12 60.5
MS.gene54347.t1 MTR_3g084210 47.692 65 30 3 64 126 20 82 2.65e-12 60.1
MS.gene54347.t1 MTR_4g072820 36.842 76 47 1 55 129 5 80 3.35e-12 59.3
MS.gene54347.t1 MTR_4g072260 37.333 75 46 1 56 129 12 86 3.89e-12 59.7
MS.gene54347.t1 MTR_4g072330 40.909 66 38 1 70 134 23 88 5.14e-12 59.3
MS.gene54347.t1 MTR_4g072640 45.902 61 32 1 70 129 25 85 5.22e-12 59.3
MS.gene54347.t1 MTR_4g025830 38.961 77 44 1 70 143 79 155 6.26e-12 60.8
MS.gene54347.t1 MTR_1g063950 31.624 117 58 2 14 130 14 108 8.46e-12 59.3
MS.gene54347.t1 MTR_3g110045 47.458 59 28 2 70 126 84 141 8.60e-12 60.1
MS.gene54347.t1 MTR_4g072910 46.552 58 30 1 70 126 35 92 1.12e-11 58.5
MS.gene54347.t1 MTR_4g072870 39.394 66 39 1 70 134 26 91 1.37e-11 58.2
MS.gene54347.t1 MTR_4g072240 36.250 80 50 1 56 134 12 91 1.46e-11 58.2
MS.gene54347.t1 MTR_7g101890 38.889 72 42 1 61 130 43 114 1.56e-11 58.5
MS.gene54347.t1 MTR_4g072890 37.333 75 46 1 56 129 12 86 1.82e-11 57.8
MS.gene54347.t1 MTR_3g110038 44.262 61 33 1 70 129 25 85 2.28e-11 57.4
MS.gene54347.t1 MTR_4g072530 47.541 61 31 1 70 129 25 85 2.77e-11 57.4
MS.gene54347.t1 MTR_3g084230 45.763 59 29 2 70 126 25 82 3.23e-11 57.0
MS.gene54347.t1 MTR_8g461420 45.763 59 29 2 70 126 25 82 3.41e-11 57.0
MS.gene54347.t1 MTR_3g110032 35.294 85 51 2 46 129 5 86 3.45e-11 57.0
MS.gene54347.t1 MTR_4g072620 47.458 59 30 1 72 129 27 85 4.94e-11 56.6
MS.gene54347.t1 MTR_4g072250 42.424 66 36 2 70 134 26 90 5.43e-11 56.6
MS.gene54347.t1 MTR_4g072860 44.262 61 33 1 70 129 26 86 5.50e-11 56.6
MS.gene54347.t1 MTR_3g084190 44.068 59 30 2 70 126 25 82 8.47e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54347.t1 AT5G50760 44.828 116 61 2 19 133 5 118 2.05e-23 91.3
MS.gene54347.t1 AT1G56150 47.887 71 37 0 60 130 28 98 4.67e-18 75.5
MS.gene54347.t1 AT5G20810 46.970 66 35 0 70 135 79 144 2.75e-17 74.7
MS.gene54347.t1 AT5G20810 46.970 66 35 0 70 135 79 144 4.02e-17 75.1
MS.gene54347.t1 AT3G43120 45.455 66 36 0 70 135 79 144 8.02e-17 73.6
MS.gene54347.t1 AT3G12830 44.048 84 44 2 49 130 28 110 3.38e-16 71.2
MS.gene54347.t1 AT2G46690 45.833 72 36 1 68 136 22 93 1.35e-15 69.3
MS.gene54347.t1 AT3G61900 41.379 87 39 2 68 142 28 114 1.72e-15 69.3
MS.gene54347.t1 AT1G16510 46.970 66 35 0 65 130 49 114 4.15e-15 68.9
MS.gene54347.t1 AT4G00880 40.964 83 45 2 56 136 16 96 1.10e-14 67.0
MS.gene54347.t1 AT4G34750 44.262 61 34 0 70 130 37 97 1.24e-14 67.4
MS.gene54347.t1 AT4G34750 44.262 61 34 0 70 130 37 97 1.24e-14 67.4
MS.gene54347.t1 AT1G75590 49.123 57 29 0 70 126 42 98 7.04e-14 65.9
MS.gene54347.t1 AT1G79130 36.111 108 56 2 26 133 17 111 1.31e-13 64.3
MS.gene54347.t1 AT5G10990 49.123 57 29 0 70 126 41 97 1.34e-13 64.7
MS.gene54347.t1 AT5G53590 42.466 73 37 1 70 137 46 118 1.68e-13 64.3
MS.gene54347.t1 AT1G19840 44.262 61 34 0 70 130 41 101 1.39e-12 62.4
MS.gene54347.t1 AT2G24400 40.230 87 50 1 52 136 46 132 1.98e-12 62.4
MS.gene54347.t1 AT4G31320 40.000 80 43 2 71 145 81 160 5.47e-12 61.2
MS.gene54347.t1 AT3G20220 38.235 68 41 1 65 131 42 109 9.40e-12 59.3
MS.gene54347.t1 AT2G37030 40.984 61 36 0 70 130 49 109 2.76e-11 58.2
MS.gene54347.t1 AT3G60690 38.542 96 52 3 58 148 74 167 3.85e-11 58.9
MS.gene54347.t1 AT3G51200 36.923 65 41 0 66 130 36 100 6.17e-11 56.6
MS.gene54347.t1 AT5G66260 36.364 66 41 1 70 135 34 98 6.42e-11 56.6
MS.gene54347.t1 AT2G36210 31.707 123 67 4 14 130 4 115 8.37e-11 57.0

Find 40 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCTTCTTAGCCCTTCC+AGG 0.273002 7.4:-93980940 MS.gene54347:CDS
CCTTCCCAAGGTTGTACTTT+TGG 0.273068 7.4:-93980980 MS.gene54347:CDS
ACCACAAACCTTTGCATTTG+AGG 0.340829 7.4:+93981151 None:intergenic
GGAAAAGGGAACAAGAAAAT+AGG 0.347522 7.4:-93981364 MS.gene54347:CDS
GAAGAAGCTGAATCAGAATA+TGG 0.364921 7.4:-93981085 MS.gene54347:CDS
TTACAAAGTGTTGATGGAAA+TGG 0.371199 7.4:-93981014 MS.gene54347:CDS
TTATAGCTGCCAAGGACCTT+TGG 0.404368 7.4:-93981062 MS.gene54347:CDS
AAGAACATGAAGCAAGGAAA+AGG 0.428717 7.4:-93981379 MS.gene54347:CDS
ACCTCAAATGCAAAGGTTTG+TGG 0.451149 7.4:-93981152 MS.gene54347:CDS
GTTGTTTAAGACAATCATCC+TGG 0.454314 7.4:+93980922 None:intergenic
GATTTGCTCCTTCTTGTCGT+TGG 0.461730 7.4:+93981253 None:intergenic
TCTTCGCCCCGCGGAAGACT+AGG 0.501811 7.4:+93981226 None:intergenic
TAAAAGACATCAACATTACA+AGG 0.510903 7.4:+93981034 None:intergenic
AGAACATGAAGCAAGGAAAA+GGG 0.512113 7.4:-93981378 MS.gene54347:CDS
TGCAAGTCCATAGGAGGAGG+AGG 0.512745 7.4:-93981316 MS.gene54347:CDS
ATCATGGCCTAGTCTTCCGC+GGG 0.516084 7.4:-93981233 MS.gene54347:CDS
AAGGAAGAGCCAAAGGTCCT+TGG 0.517712 7.4:+93981053 None:intergenic
CGACAAGAAGGAGCAAATCA+TGG 0.519386 7.4:-93981249 MS.gene54347:CDS
TAGACAAAAGAAAGTGGCCC+CGG 0.522285 7.4:-93981200 MS.gene54347:CDS
TCAAGCAACATCTTGAACAA+AGG 0.532123 7.4:+93981106 None:intergenic
ACGACCAAAAGTACAACCTT+GGG 0.533998 7.4:+93980976 None:intergenic
AACGACCAAAAGTACAACCT+TGG 0.538821 7.4:+93980975 None:intergenic
AGATGCAAGTCCATAGGAGG+AGG 0.541895 7.4:-93981319 MS.gene54347:CDS
AAGTCCATAGGAGGAGGAGG+AGG 0.549753 7.4:-93981313 MS.gene54347:CDS
AGAAAATAGACAAAAGAAAG+TGG 0.550670 7.4:-93981206 MS.gene54347:CDS
GAATATGGTTATAGCTGCCA+AGG 0.552741 7.4:-93981070 MS.gene54347:CDS
CAAAAGAAAGTGGCCCCGGA+GGG 0.557663 7.4:-93981196 MS.gene54347:CDS
CAGTGATTCCAACGACAAGA+AGG 0.563887 7.4:-93981261 MS.gene54347:CDS
TTTAAGACAATCATCCTGGA+AGG 0.580942 7.4:+93980926 None:intergenic
TGGGAGAGATGCAAGTCCAT+AGG 0.597578 7.4:-93981325 MS.gene54347:CDS
AATCATGGCCTAGTCTTCCG+CGG 0.600149 7.4:-93981234 MS.gene54347:CDS
GAGAGATGCAAGTCCATAGG+AGG 0.601664 7.4:-93981322 MS.gene54347:CDS
TTAAGACAATCATCCTGGAA+GGG 0.611880 7.4:+93980927 None:intergenic
ACATTACAAGGAAGAGCCAA+AGG 0.626356 7.4:+93981046 None:intergenic
ACGTTGGACCTCAAATGCAA+AGG 0.634178 7.4:-93981159 MS.gene54347:CDS
ATTGGCAAGAACATGAAGCA+AGG 0.634830 7.4:-93981385 MS.gene54347:CDS
GATAACGAAGCTCCTTCCCA+AGG 0.635029 7.4:-93980992 MS.gene54347:CDS
ACAAAAGAAAGTGGCCCCGG+AGG 0.644682 7.4:-93981197 MS.gene54347:CDS
CCAAAAGTACAACCTTGGGA+AGG 0.674764 7.4:+93980980 None:intergenic
TCATGGCCTAGTCTTCCGCG+GGG 0.775767 7.4:-93981232 MS.gene54347:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAATAGACAAAAGAAAG+TGG - chr7.4:93981101-93981120 MS.gene54347:CDS 25.0%
! TAAAAGACATCAACATTACA+AGG + chr7.4:93981276-93981295 None:intergenic 25.0%
!! TTACAAAGTGTTGATGGAAA+TGG - chr7.4:93981293-93981312 MS.gene54347:CDS 30.0%
!!! TGTCTTTTACAAAGTGTTGA+TGG - chr7.4:93981287-93981306 MS.gene54347:CDS 30.0%
AAGAACATGAAGCAAGGAAA+AGG - chr7.4:93980928-93980947 MS.gene54347:CDS 35.0%
AGAACATGAAGCAAGGAAAA+GGG - chr7.4:93980929-93980948 MS.gene54347:CDS 35.0%
GAAGAAGCTGAATCAGAATA+TGG - chr7.4:93981222-93981241 MS.gene54347:CDS 35.0%
GGAAAAGGGAACAAGAAAAT+AGG - chr7.4:93980943-93980962 MS.gene54347:CDS 35.0%
TCAAGCAACATCTTGAACAA+AGG + chr7.4:93981204-93981223 None:intergenic 35.0%
TTAAGACAATCATCCTGGAA+GGG + chr7.4:93981383-93981402 None:intergenic 35.0%
! GTTGTTTAAGACAATCATCC+TGG + chr7.4:93981388-93981407 None:intergenic 35.0%
! TTTAAGACAATCATCCTGGA+AGG + chr7.4:93981384-93981403 None:intergenic 35.0%
ACATTACAAGGAAGAGCCAA+AGG + chr7.4:93981264-93981283 None:intergenic 40.0%
ACCACAAACCTTTGCATTTG+AGG + chr7.4:93981159-93981178 None:intergenic 40.0%
ACCTCAAATGCAAAGGTTTG+TGG - chr7.4:93981155-93981174 MS.gene54347:CDS 40.0%
AGGCCTCATAACAAAAACGT+GGG - chr7.4:93980963-93980982 MS.gene54347:CDS 40.0%
ATTGGCAAGAACATGAAGCA+AGG - chr7.4:93980922-93980941 MS.gene54347:CDS 40.0%
GAATATGGTTATAGCTGCCA+AGG - chr7.4:93981237-93981256 MS.gene54347:CDS 40.0%
TAGGCCTCATAACAAAAACG+TGG - chr7.4:93980962-93980981 MS.gene54347:CDS 40.0%
! AACGACCAAAAGTACAACCT+TGG + chr7.4:93981335-93981354 None:intergenic 40.0%
! ACGACCAAAAGTACAACCTT+GGG + chr7.4:93981334-93981353 None:intergenic 40.0%
!! TCTCCCACGTTTTTGTTATG+AGG + chr7.4:93980969-93980988 None:intergenic 40.0%
ACGTTGGACCTCAAATGCAA+AGG - chr7.4:93981148-93981167 MS.gene54347:CDS 45.0%
ATCATCTTCTTAGCCCTTCC+AGG - chr7.4:93981367-93981386 MS.gene54347:CDS 45.0%
CAGTGATTCCAACGACAAGA+AGG - chr7.4:93981046-93981065 MS.gene54347:CDS 45.0%
CGACAAGAAGGAGCAAATCA+TGG - chr7.4:93981058-93981077 MS.gene54347:CDS 45.0%
TACACCGAAAAACATCCCTC+CGG + chr7.4:93981129-93981148 None:intergenic 45.0%
TAGACAAAAGAAAGTGGCCC+CGG - chr7.4:93981107-93981126 MS.gene54347:CDS 45.0%
TTATAGCTGCCAAGGACCTT+TGG - chr7.4:93981245-93981264 MS.gene54347:CDS 45.0%
! CCAAAAGTACAACCTTGGGA+AGG + chr7.4:93981330-93981349 None:intergenic 45.0%
! CCTTCCCAAGGTTGTACTTT+TGG - chr7.4:93981327-93981346 MS.gene54347:CDS 45.0%
! GATTTGCTCCTTCTTGTCGT+TGG + chr7.4:93981057-93981076 None:intergenic 45.0%
!! GGATGTTTTTCGGTGTACGT+TGG - chr7.4:93981132-93981151 MS.gene54347:CDS 45.0%
AAGGAAGAGCCAAAGGTCCT+TGG + chr7.4:93981257-93981276 None:intergenic 50.0%
ACACCGAAAAACATCCCTCC+GGG + chr7.4:93981128-93981147 None:intergenic 50.0%
AGATGCAAGTCCATAGGAGG+AGG - chr7.4:93980988-93981007 MS.gene54347:CDS 50.0%
GAGAGATGCAAGTCCATAGG+AGG - chr7.4:93980985-93981004 MS.gene54347:CDS 50.0%
GATAACGAAGCTCCTTCCCA+AGG - chr7.4:93981315-93981334 MS.gene54347:CDS 50.0%
TGGGAGAGATGCAAGTCCAT+AGG - chr7.4:93980982-93981001 MS.gene54347:CDS 50.0%
! AATCATGGCCTAGTCTTCCG+CGG - chr7.4:93981073-93981092 MS.gene54347:CDS 50.0%
! TGTCTATTTTCTTCGCCCCG+CGG + chr7.4:93981093-93981112 None:intergenic 50.0%
AAGTCCATAGGAGGAGGAGG+AGG - chr7.4:93980994-93981013 MS.gene54347:CDS 55.0%
ACAAAAGAAAGTGGCCCCGG+AGG - chr7.4:93981110-93981129 MS.gene54347:CDS 55.0%
CAAAAGAAAGTGGCCCCGGA+GGG - chr7.4:93981111-93981130 MS.gene54347:CDS 55.0%
CACCGAAAAACATCCCTCCG+GGG + chr7.4:93981127-93981146 None:intergenic 55.0%
TGCAAGTCCATAGGAGGAGG+AGG - chr7.4:93980991-93981010 MS.gene54347:CDS 55.0%
! ATCATGGCCTAGTCTTCCGC+GGG - chr7.4:93981074-93981093 MS.gene54347:CDS 55.0%
TCCATAGGAGGAGGAGGAGG+AGG - chr7.4:93980997-93981016 MS.gene54347:CDS 60.0%
TCCTCCTCCTCCTCCTCCTA+TGG + chr7.4:93981001-93981020 None:intergenic 60.0%
! GGCCCCGGAGGGATGTTTTT+CGG - chr7.4:93981122-93981141 MS.gene54347:CDS 60.0%
! TCATGGCCTAGTCTTCCGCG+GGG - chr7.4:93981075-93981094 MS.gene54347:CDS 60.0%
TCTTCGCCCCGCGGAAGACT+AGG + chr7.4:93981084-93981103 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 93980916 93981413 93980916 ID=MS.gene54347
chr7.4 mRNA 93980916 93981413 93980916 ID=MS.gene54347.t1;Parent=MS.gene54347
chr7.4 exon 93980916 93981413 93980916 ID=MS.gene54347.t1.exon1;Parent=MS.gene54347.t1
chr7.4 CDS 93980916 93981413 93980916 ID=cds.MS.gene54347.t1;Parent=MS.gene54347.t1
Gene Sequence

>MS.gene54347

ATGGATATTGGCAAGAACATGAAGCAAGGAAAAGGGAACAAGAAAATAGGCCTCATAACAAAAACGTGGGAGAGATGCAAGTCCATAGGAGGAGGAGGAGGAGGACACAAGAGTAAATCATATTCATCATCAGTGATTCCAACGACAAGAAGGAGCAAATCATGGCCTAGTCTTCCGCGGGGCGAAGAAAATAGACAAAAGAAAGTGGCCCCGGAGGGATGTTTTTCGGTGTACGTTGGACCTCAAATGCAAAGGTTTGTGGTCAAAACTGAGTATGCTAATCATCCTTTGTTCAAGATGTTGCTTGAAGAAGCTGAATCAGAATATGGTTATAGCTGCCAAGGACCTTTGGCTCTTCCTTGTAATGTTGATGTCTTTTACAAAGTGTTGATGGAAATGGATAACGAAGCTCCTTCCCAAGGTTGTACTTTTGGTCGTTCTCGTTCTTCTTATCATCTTCTTAGCCCTTCCAGGATGATTGTCTTAAACAACTTCTAA

Protein sequence

>MS.gene54347.t1

MDIGKNMKQGKGNKKIGLITKTWERCKSIGGGGGGHKSKSYSSSVIPTTRRSKSWPSLPRGEENRQKKVAPEGCFSVYVGPQMQRFVVKTEYANHPLFKMLLEEAESEYGYSCQGPLALPCNVDVFYKVLMEMDNEAPSQGCTFGRSRSSYHLLSPSRMIVLNNF