AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065072


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065072.t1 MTR_1g111840 96.104 77 3 0 1 77 1 77 1.08e-49 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065072.t1 AT5G04750 58.730 63 23 3 13 73 25 86 4.37e-13 60.1

Find 31 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTGGTACCTTGTTAT+CGG 0.335217 1.2:+82806173 None:intergenic
ATCTGTAGGTTTCTCCGCCT+TGG 0.365908 1.2:+82806200 None:intergenic
CACAACTGCGCACCATGGTA+AGG 0.400797 1.2:+82804945 None:intergenic
CAAAGAAAACGTCTACATTC+AGG 0.413338 1.2:-82806350 MS.gene065072:intron
GAAATGGGAGAGGGAAAGGT+TGG 0.423182 1.2:-82806250 MS.gene065072:CDS
ACAGAAGCTAGCGGCTGATA+AGG 0.427012 1.2:-82806223 MS.gene065072:CDS
ATGCGCCTTCCAAGCACAGC+AGG 0.431050 1.2:-82804980 MS.gene065072:CDS
AGGGATCGTGACTCGTTCAA+TGG 0.456515 1.2:-82806446 MS.gene065072:CDS
TTGTTATCGGCATTATCTGT+AGG 0.488471 1.2:+82806186 None:intergenic
TGTCCATTGCAAATTGTTTG+TGG 0.522812 1.2:-82805004 MS.gene065072:CDS
TCTCGCTTCTTCAGCGGCAA+AGG 0.525127 1.2:-82806400 MS.gene065072:CDS
TTTGTGTGTAGAAATGGGAG+AGG 0.537501 1.2:-82806260 MS.gene065072:intron
TTGTGTGTAGAAATGGGAGA+GGG 0.546268 1.2:-82806259 MS.gene065072:intron
TGTAGAAATGGGAGAGGGAA+AGG 0.549281 1.2:-82806254 MS.gene065072:intron
TGCGCCTTCCAAGCACAGCA+GGG 0.549426 1.2:-82804979 MS.gene065072:CDS
ACAACTGCGCACCATGGTAA+GGG 0.550073 1.2:+82804946 None:intergenic
CGCCTTCCAAGCACAGCAGG+GGG 0.563933 1.2:-82804977 MS.gene065072:CDS
GCGCCTTCCAAGCACAGCAG+GGG 0.568690 1.2:-82804978 MS.gene065072:CDS
GGGATGATTGCCCCTTACCA+TGG 0.579577 1.2:-82804957 MS.gene065072:CDS
CAACTGCGCACCATGGTAAG+GGG 0.587511 1.2:+82804947 None:intergenic
TACAGATAATGCCGATAACA+AGG 0.591322 1.2:-82806184 MS.gene065072:intron
TCAATGGATTCAACATCTCG+TGG 0.600776 1.2:-82806430 MS.gene065072:CDS
TGAGATCAGCACTGAGTCGA+GGG 0.604557 1.2:-82806465 MS.gene065072:CDS
ATGAGATCAGCACTGAGTCG+AGG 0.608842 1.2:-82806466 MS.gene065072:CDS
GCTTCTTCTCGCTTCTTCAG+CGG 0.618308 1.2:-82806406 MS.gene065072:CDS
ATGCCACAAACAATTTGCAA+TGG 0.640316 1.2:+82805001 None:intergenic
GCTAGCGGCTGATAAGGCCA+AGG 0.640541 1.2:-82806217 MS.gene065072:CDS
CAAAGGTAAGATTCTTAGCG+AGG 0.644514 1.2:-82806383 MS.gene065072:CDS
AGCGGCTGATAAGGCCAAGG+CGG 0.664107 1.2:-82806214 MS.gene065072:CDS
AAGCGCACAACTGCGCACCA+TGG 0.674306 1.2:+82804940 None:intergenic
GTTGGAGAAACAGAAGCTAG+CGG 0.726434 1.2:-82806232 MS.gene065072:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCTAAAAACAAAATAATT+TGG + chr1.2:82805254-82805273 None:intergenic 15.0%
!!! AATTTTAAAAGTGAAGAATT+AGG + chr1.2:82806296-82806315 None:intergenic 15.0%
!!! AATTTTAAGATATGAATCAT+AGG + chr1.2:82805482-82805501 None:intergenic 15.0%
!!! TACCAAATTATTTTGTTTTT+AGG - chr1.2:82805249-82805268 MS.gene065072:intron 15.0%
!! ATATCAAAGTTACAGAATAT+TGG + chr1.2:82805564-82805583 None:intergenic 20.0%
!! ATATTAATAGATCACAAATC+TGG + chr1.2:82805322-82805341 None:intergenic 20.0%
!! TAAAAAAGGATGAAGAAAAA+GGG + chr1.2:82805098-82805117 None:intergenic 20.0%
!! TAACTTTGATATGAATATGA+TGG - chr1.2:82805572-82805591 MS.gene065072:intron 20.0%
!! TATCAAAGTTACAGAATATT+GGG + chr1.2:82805563-82805582 None:intergenic 20.0%
!!! ATATTTCTTTCTGCATATTT+TGG - chr1.2:82805284-82805303 MS.gene065072:intron 20.0%
!!! TATTTCTTTCTGCATATTTT+GGG - chr1.2:82805285-82805304 MS.gene065072:intron 20.0%
!!! TTTGAAATTGTATCATATGA+TGG - chr1.2:82805454-82805473 MS.gene065072:intron 20.0%
!!! TTTTTGATTTGGTGTATTTT+TGG - chr1.2:82805375-82805394 MS.gene065072:intron 20.0%
! AAAAAAGGATGAAGAAAAAG+GGG + chr1.2:82805097-82805116 None:intergenic 25.0%
! AATTCGATTTCATTGAAACT+TGG + chr1.2:82805910-82805929 None:intergenic 25.0%
! AGAAAGAAAGAAAAAAAGGA+AGG + chr1.2:82806119-82806138 None:intergenic 25.0%
! AGAATTTAAGAAGCTAAAAG+TGG + chr1.2:82805618-82805637 None:intergenic 25.0%
! ATTCGATTTCATTGAAACTT+GGG + chr1.2:82805909-82805928 None:intergenic 25.0%
! CAAGAAATTGGATCTAAAAA+AGG + chr1.2:82805112-82805131 None:intergenic 25.0%
! CTAAAAAAGGATGAAGAAAA+AGG + chr1.2:82805099-82805118 None:intergenic 25.0%
! GAAAGAAAGAAAAAAAGGAA+GGG + chr1.2:82806118-82806137 None:intergenic 25.0%
! TCAAAAAATCATCTCAGAAT+TGG + chr1.2:82805362-82805381 None:intergenic 25.0%
!! ATAATTTGGTACCTTGTTAT+CGG + chr1.2:82805240-82805259 None:intergenic 25.0%
!! TGATTTTTGTGTGTAGAAAT+GGG - chr1.2:82805145-82805164 MS.gene065072:intron 25.0%
!! TTGATTTTTGTGTGTAGAAA+TGG - chr1.2:82805144-82805163 MS.gene065072:intron 25.0%
!!! CTTTTAGCTTCTTAAATTCT+TGG - chr1.2:82805618-82805637 MS.gene065072:intron 25.0%
!!! GAATTTTGTTTCTTTTACCA+AGG - chr1.2:82805646-82805665 MS.gene065072:intron 25.0%
!!! TCCTTTTTTTCTTTCTTTCT+GGG - chr1.2:82806119-82806138 MS.gene065072:intron 25.0%
!!! TCTGAGATGATTTTTTGATT+TGG - chr1.2:82805364-82805383 MS.gene065072:intron 25.0%
!!! TTCCTTTTTTTCTTTCTTTC+TGG - chr1.2:82806118-82806137 MS.gene065072:intron 25.0%
!!! TTTAGTTGATAGCAAAATTG+TGG + chr1.2:82806233-82806252 None:intergenic 25.0%
AAAAAGGATGAAGAAAAAGG+GGG + chr1.2:82805096-82805115 None:intergenic 30.0%
AAATCGAGTGAAACTAGAAA+TGG - chr1.2:82805939-82805958 MS.gene065072:intron 30.0%
AATTCGATTTCACTGTCATA+TGG - chr1.2:82805746-82805765 MS.gene065072:intron 30.0%
AGTTGTGTATAGTGTAAAAG+AGG + chr1.2:82806169-82806188 None:intergenic 30.0%
ATTGGTTACTCTTTCCAAAA+AGG + chr1.2:82806069-82806088 None:intergenic 30.0%
CCAAAAATATGTTCAAACCT+TGG + chr1.2:82805666-82805685 None:intergenic 30.0%
TAGTTAATGACTAAACAGTG+AGG + chr1.2:82805829-82805848 None:intergenic 30.0%
TAGTTTCACTCGATTTCTAA+AGG + chr1.2:82805935-82805954 None:intergenic 30.0%
TTGGTTACTCTTTCCAAAAA+GGG + chr1.2:82806068-82806087 None:intergenic 30.0%
! AAGCAACACACTCTTTTTAA+AGG - chr1.2:82805797-82805816 MS.gene065072:intron 30.0%
! AATTTATGCTGTACCCTTTT+TGG - chr1.2:82806052-82806071 MS.gene065072:intron 30.0%
! AGCAACACACTCTTTTTAAA+GGG - chr1.2:82805798-82805817 MS.gene065072:intron 30.0%
! CCAAGGTTTGAACATATTTT+TGG - chr1.2:82805663-82805682 MS.gene065072:intron 30.0%
! TCTTTTACACTATACACAAC+TGG - chr1.2:82806168-82806187 MS.gene065072:intron 30.0%
!!! CCTTTTTTTCTTTCTTTCTG+GGG - chr1.2:82806120-82806139 MS.gene065072:intron 30.0%
!!! GAACATATTTTTGGTTAACG+TGG - chr1.2:82805672-82805691 MS.gene065072:intron 30.0%
!!! GTATGCTTTAGCACATTTTT+TGG - chr1.2:82806354-82806373 MS.gene065072:CDS 30.0%
AACTAGAAATGGAGTTGGAA+TGG - chr1.2:82805950-82805969 MS.gene065072:intron 35.0%
AGAATATTGGGAGCTACAAA+AGG + chr1.2:82805551-82805570 None:intergenic 35.0%
AGAGTAACCAATGTTAGACT+TGG - chr1.2:82806077-82806096 MS.gene065072:intron 35.0%
AGTGAAACTAGAAATGGAGT+TGG - chr1.2:82805945-82805964 MS.gene065072:intron 35.0%
ATGCCACAAACAATTTGCAA+TGG + chr1.2:82806412-82806431 None:intergenic 35.0%
CAAAGAAAACGTCTACATTC+AGG - chr1.2:82805060-82805079 MS.gene065072:intron 35.0%
CCCCAGAAAGAAAGAAAAAA+AGG + chr1.2:82806123-82806142 None:intergenic 35.0%
GTTAGTACCAAGTCTAACAT+TGG + chr1.2:82806087-82806106 None:intergenic 35.0%
TACAGATAATGCCGATAACA+AGG - chr1.2:82805226-82805245 MS.gene065072:intron 35.0%
TGTCCATTGCAAATTGTTTG+TGG - chr1.2:82806406-82806425 MS.gene065072:CDS 35.0%
TGTGTATAGTGTAAAAGAGG+TGG + chr1.2:82806166-82806185 None:intergenic 35.0%
TTGTGGAGAGATGAATGTAA+AGG + chr1.2:82806216-82806235 None:intergenic 35.0%
TTGTTATCGGCATTATCTGT+AGG + chr1.2:82805227-82805246 None:intergenic 35.0%
! AATTAGGTTGCAGTCGTTTA+AGG + chr1.2:82806280-82806299 None:intergenic 35.0%
! ATTAGGTTGCAGTCGTTTAA+GGG + chr1.2:82806279-82806298 None:intergenic 35.0%
! CCAATTTCTTGCTTCTGAAA+CGG - chr1.2:82805121-82805140 MS.gene065072:intron 35.0%
! CTGAATGTAGACGTTTTCTT+TGG + chr1.2:82805062-82805081 None:intergenic 35.0%
!! CAACACACTCTTTTTAAAGG+GGG - chr1.2:82805800-82805819 MS.gene065072:intron 35.0%
!! GCAACACACTCTTTTTAAAG+GGG - chr1.2:82805799-82805818 MS.gene065072:intron 35.0%
ATTCAATGAAACACGACCAC+AGG + chr1.2:82805729-82805748 None:intergenic 40.0%
CAAAGGTAAGATTCTTAGCG+AGG - chr1.2:82805027-82805046 MS.gene065072:intron 40.0%
CCGTTTCAGAAGCAAGAAAT+TGG + chr1.2:82805124-82805143 None:intergenic 40.0%
GAAATGGAGTTGGAATGGTA+AGG - chr1.2:82805955-82805974 MS.gene065072:intron 40.0%
TCGATTTCACTGTCATATGG+TGG - chr1.2:82805749-82805768 MS.gene065072:intron 40.0%
TTCCATGTTGCTGTGAATCA+AGG - chr1.2:82806383-82806402 MS.gene065072:CDS 40.0%
TTGTGTGTAGAAATGGGAGA+GGG - chr1.2:82805151-82805170 MS.gene065072:intron 40.0%
TTTGTGTGTAGAAATGGGAG+AGG - chr1.2:82805150-82805169 MS.gene065072:intron 40.0%
! CTACGAACACACTCTTTTGA+AGG - chr1.2:82805850-82805869 MS.gene065072:intron 40.0%
! TCAATGGATTCAACATCTCG+TGG - chr1.2:82804980-82804999 MS.gene065072:CDS 40.0%
!! AAAAGAGTGTGTTGCTTGCT+AGG + chr1.2:82805794-82805813 None:intergenic 40.0%
!! CACTCTTTTGAAGGTGGATT+TGG - chr1.2:82805859-82805878 MS.gene065072:intron 40.0%
!! TTGAAGGTGGATTTGGCTTT+CGG - chr1.2:82805866-82805885 MS.gene065072:intron 40.0%
!! TTTTCTTTCTTTCTGGGGAG+TGG - chr1.2:82806125-82806144 MS.gene065072:intron 40.0%
CACCTTGATTCACAGCAACA+TGG + chr1.2:82806388-82806407 None:intergenic 45.0%
GTTGGAGAAACAGAAGCTAG+CGG - chr1.2:82805178-82805197 MS.gene065072:intron 45.0%
TGTAGAAATGGGAGAGGGAA+AGG - chr1.2:82805156-82805175 MS.gene065072:intron 45.0%
! ACGTGGTGATGACATGATTC+TGG - chr1.2:82805701-82805720 MS.gene065072:intron 45.0%
!! CGAACACACTCTTTTGAAGG+TGG - chr1.2:82805853-82805872 MS.gene065072:intron 45.0%
!! TGACATGATTCTGGAACCTG+TGG - chr1.2:82805710-82805729 MS.gene065072:intron 45.0%
ACAACTGCGCACCATGGTAA+GGG + chr1.2:82806467-82806486 None:intergenic 50.0%
ACAGAAGCTAGCGGCTGATA+AGG - chr1.2:82805187-82805206 MS.gene065072:intron 50.0%
AGGGATCGTGACTCGTTCAA+TGG - chr1.2:82804964-82804983 MS.gene065072:CDS 50.0%
ATCTGTAGGTTTCTCCGCCT+TGG + chr1.2:82805213-82805232 None:intergenic 50.0%
GAAATGGGAGAGGGAAAGGT+TGG - chr1.2:82805160-82805179 MS.gene065072:intron 50.0%
GCTTCTTCTCGCTTCTTCAG+CGG - chr1.2:82805004-82805023 MS.gene065072:CDS 50.0%
GGTTAACGTGGTATTGCACG+TGG - chr1.2:82805684-82805703 MS.gene065072:intron 50.0%
TGGAGTTGGAATGGTAAGGC+AGG - chr1.2:82805959-82805978 MS.gene065072:intron 50.0%
! ATGAGATCAGCACTGAGTCG+AGG - chr1.2:82804944-82804963 MS.gene065072:CDS 50.0%
! TGAGATCAGCACTGAGTCGA+GGG - chr1.2:82804945-82804964 MS.gene065072:CDS 50.0%
AATCATCCCCCTGCTGTGCT+TGG + chr1.2:82806442-82806461 None:intergenic 55.0%
CAACTGCGCACCATGGTAAG+GGG + chr1.2:82806466-82806485 None:intergenic 55.0%
CACAACTGCGCACCATGGTA+AGG + chr1.2:82806468-82806487 None:intergenic 55.0%
GGGATGATTGCCCCTTACCA+TGG - chr1.2:82806453-82806472 MS.gene065072:CDS 55.0%
TCTCGCTTCTTCAGCGGCAA+AGG - chr1.2:82805010-82805029 MS.gene065072:intron 55.0%
AAGCGCACAACTGCGCACCA+TGG + chr1.2:82806473-82806492 None:intergenic 60.0%
AGCGGCTGATAAGGCCAAGG+CGG - chr1.2:82805196-82805215 MS.gene065072:intron 60.0%
ATCCCCCTGCTGTGCTTGGA+AGG + chr1.2:82806438-82806457 None:intergenic 60.0%
ATGCGCCTTCCAAGCACAGC+AGG - chr1.2:82806430-82806449 MS.gene065072:CDS 60.0%
GCTAGCGGCTGATAAGGCCA+AGG - chr1.2:82805193-82805212 MS.gene065072:intron 60.0%
TGCGCCTTCCAAGCACAGCA+GGG - chr1.2:82806431-82806450 MS.gene065072:CDS 60.0%
CGCCTTCCAAGCACAGCAGG+GGG - chr1.2:82806433-82806452 MS.gene065072:CDS 65.0%
GCGCCTTCCAAGCACAGCAG+GGG - chr1.2:82806432-82806451 MS.gene065072:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 82804938 82806494 82804938 ID=MS.gene065072
chr1.2 mRNA 82804938 82806494 82804938 ID=MS.gene065072.t1;Parent=MS.gene065072
chr1.2 exon 82806351 82806494 82806351 ID=MS.gene065072.t1.exon1;Parent=MS.gene065072.t1
chr1.2 CDS 82806351 82806494 82806351 ID=cds.MS.gene065072.t1;Parent=MS.gene065072.t1
chr1.2 exon 82806185 82806271 82806185 ID=MS.gene065072.t1.exon2;Parent=MS.gene065072.t1
chr1.2 CDS 82806185 82806271 82806185 ID=cds.MS.gene065072.t1;Parent=MS.gene065072.t1
chr1.2 exon 82804938 82805027 82804938 ID=MS.gene065072.t1.exon3;Parent=MS.gene065072.t1
chr1.2 CDS 82804938 82805027 82804938 ID=cds.MS.gene065072.t1;Parent=MS.gene065072.t1
Gene Sequence

>MS.gene065072

ATGGCAATGAGATCAGCACTGAGTCGAGGGATCGTGACTCGTTCAATGGATTCAACATCTCGTGGAGCTTCTTCTCGCTTCTTCAGCGGCAAAGGTAAGATTCTTAGCGAGGAAGAACAAGCCAAAGAAAACGTCTACATTCAGGTACTTCATTTCCCCCTTTTTCTTCATCCTTTTTTAGATCCAATTTCTTGCTTCTGAAACGGTTGATTTTTGTGTGTAGAAATGGGAGAGGGAAAGGTTGGAGAAACAGAAGCTAGCGGCTGATAAGGCCAAGGCGGAGAAACCTACAGATAATGCCGATAACAAGGTACCAAATTATTTTGTTTTTAGGTTATTGATTTCAATATTTCTTTCTGCATATTTTGGGTCTGATTAAAACCAGATTTGTGATCTATTAATATTATTATTGTGTTAGTTACCAATTCTGAGATGATTTTTTGATTTGGTGTATTTTTGGATTTGTTACTTTCTGTTTAGTGCAAATTTTAATTTCATTTTTTAGTTTTGAAGCTATTTGAAATTGTATCATATGATGGTTCCTATGATTCATATCTTAAAATTTATTCAATTTTTTTTACGCATTAAGATGTTCAGCTAAATGACAATTCCTTTTGTAGCTCCCAATATTCTGTAACTTTGATATGAATATGATGGATGTTATTTTTTTTGTTTCACCACTTTTAGCTTCTTAAATTCTTGGATATCGAATTTTGTTTCTTTTACCAAGGTTTGAACATATTTTTGGTTAACGTGGTATTGCACGTGGTGATGACATGATTCTGGAACCTGTGGTCGTGTTTCATTGAATTCGATTTCACTGTCATATGGTGGATTCATTGTTTAGATATTACCTAGCAAGCAACACACTCTTTTTAAAGGGGGAAGCCTCACTGTTTAGTCATTAACTAGCTACGAACACACTCTTTTGAAGGTGGATTTGGCTTTCGGTGAAACTTGTAATTTGTCCCAAGTTTCAATGAAATCGAATTATCCTTTAGAAATCGAGTGAAACTAGAAATGGAGTTGGAATGGTAAGGCAGGAGTAGTAATAAATTATAATAGCATTCTTCAAAACAAACACGTTATTTTGTAATTTGTCACATTTTTTAGAAATTTATGCTGTACCCTTTTTGGAAAGAGTAACCAATGTTAGACTTGGTACTAACTAGCTAGACCCTTCCTTTTTTTCTTTCTTTCTGGGGAGTGGATTGCAAGTTGTCTACCACCTCTTTTACACTATACACAACTGGAATATTTACAGAATGAGTCAAACCTTTACATTCATCTCTCCACAATTTTGCTATCAACTAAATGCTATGTTTAATGAAATGATATCCCTTAAACGACTGCAACCTAATTCTTCACTTTTAAAATTCTGATGTTTGACACAGATTTTTTCTTGCTAAATAATTTGTATGCTTTAGCACATTTTTTGGTTATAATTCCATGTTGCTGTGAATCAAGGTGTCCATTGCAAATTGTTTGTGGCATGCGCCTTCCAAGCACAGCAGGGGGATGATTGCCCCTTACCATGGTGCGCAGTTGTGCGCTTAA

Protein sequence

>MS.gene065072.t1

MAMRSALSRGIVTRSMDSTSRGASSRFFSGKGKILSEEEQAKENVYIQKWERERLEKQKLAADKAKAEKPTDNADNKVSIANCLWHAPSKHSRGMIAPYHGAQLCA