AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76248


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76248.t1 MTR_1g111840 96.104 77 3 0 1 77 1 77 1.08e-49 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76248.t1 AT5G04750 58.730 63 23 3 13 73 25 86 4.37e-13 60.1

Find 31 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTGGTACCTTGTTAT+CGG 0.335217 1.1:+78957042 None:intergenic
ATCTGTAGGTTTCTCCGCCT+TGG 0.365908 1.1:+78957069 None:intergenic
CACAACTGCGCACCATGGTA+AGG 0.400797 1.1:+78955818 None:intergenic
CAAAGAAAACGTCTACATTC+AGG 0.413338 1.1:-78957219 MS.gene76248:intron
GAAATGGGAGAGGGAAAGGT+TGG 0.423182 1.1:-78957119 MS.gene76248:CDS
ACAGAAGCTAGCGGCTGATA+AGG 0.427012 1.1:-78957092 MS.gene76248:CDS
ATGCGCCTTCCAAGCACAGC+AGG 0.431050 1.1:-78955853 MS.gene76248:CDS
AGGGATCGTGACTCGTTCAA+TGG 0.456515 1.1:-78957315 MS.gene76248:CDS
TTGTTATCGGCATTATCTGT+AGG 0.488471 1.1:+78957055 None:intergenic
TGTCCATTGCAAATTGTTTG+TGG 0.522812 1.1:-78955877 MS.gene76248:CDS
TCTCGCTTCTTCAGCGGCAA+AGG 0.525127 1.1:-78957269 MS.gene76248:CDS
TTTGTGTGTAGAAATGGGAG+AGG 0.537501 1.1:-78957129 MS.gene76248:intron
TTGTGTGTAGAAATGGGAGA+GGG 0.546268 1.1:-78957128 MS.gene76248:intron
TGTAGAAATGGGAGAGGGAA+AGG 0.549281 1.1:-78957123 MS.gene76248:intron
TGCGCCTTCCAAGCACAGCA+GGG 0.549426 1.1:-78955852 MS.gene76248:CDS
ACAACTGCGCACCATGGTAA+GGG 0.550073 1.1:+78955819 None:intergenic
CGCCTTCCAAGCACAGCAGG+GGG 0.563933 1.1:-78955850 MS.gene76248:CDS
GCGCCTTCCAAGCACAGCAG+GGG 0.568690 1.1:-78955851 MS.gene76248:CDS
GGGATGATTGCCCCTTACCA+TGG 0.579577 1.1:-78955830 MS.gene76248:CDS
CAACTGCGCACCATGGTAAG+GGG 0.587511 1.1:+78955820 None:intergenic
TACAGATAATGCCGATAACA+AGG 0.591322 1.1:-78957053 MS.gene76248:intron
TCAATGGATTCAACATCTCG+TGG 0.600776 1.1:-78957299 MS.gene76248:CDS
TGAGATCAGCACTGAGTCGA+GGG 0.604557 1.1:-78957334 MS.gene76248:CDS
ATGAGATCAGCACTGAGTCG+AGG 0.608842 1.1:-78957335 MS.gene76248:CDS
GCTTCTTCTCGCTTCTTCAG+CGG 0.618308 1.1:-78957275 MS.gene76248:CDS
ATGCCACAAACAATTTGCAA+TGG 0.640316 1.1:+78955874 None:intergenic
GCTAGCGGCTGATAAGGCCA+AGG 0.640541 1.1:-78957086 MS.gene76248:CDS
CAAAGGTAAGATTCTTAGCG+AGG 0.644514 1.1:-78957252 MS.gene76248:CDS
AGCGGCTGATAAGGCCAAGG+CGG 0.664107 1.1:-78957083 MS.gene76248:CDS
AAGCGCACAACTGCGCACCA+TGG 0.674306 1.1:+78955813 None:intergenic
GTTGGAGAAACAGAAGCTAG+CGG 0.726434 1.1:-78957101 MS.gene76248:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCTAAAAACAAAATAATT+TGG + chr1.1:78956127-78956146 None:intergenic 15.0%
!!! AATTTTAAAAGTGAAGAATT+TGG + chr1.1:78957165-78957184 None:intergenic 15.0%
!!! AATTTTAAGATATGAATCAT+AGG + chr1.1:78956355-78956374 None:intergenic 15.0%
!!! TACCAAATTATTTTGTTTTT+AGG - chr1.1:78956122-78956141 MS.gene76248:intron 15.0%
!! ATATCAAAGTTACAGAATAT+TGG + chr1.1:78956437-78956456 None:intergenic 20.0%
!! ATATTAATAGATCACAAATC+TGG + chr1.1:78956195-78956214 None:intergenic 20.0%
!! TAAAAAAGGATGAAGAAAAA+GGG + chr1.1:78955971-78955990 None:intergenic 20.0%
!! TAACTTTGATATGAATATGA+TGG - chr1.1:78956445-78956464 MS.gene76248:intron 20.0%
!! TATCAAAGTTACAGAATATT+GGG + chr1.1:78956436-78956455 None:intergenic 20.0%
!!! ATATTTCTTTCTGCATATTT+TGG - chr1.1:78956157-78956176 MS.gene76248:intron 20.0%
!!! TATTTCTTTCTGCATATTTT+GGG - chr1.1:78956158-78956177 MS.gene76248:intron 20.0%
!!! TTTGAAATTGTATCATATGA+TGG - chr1.1:78956327-78956346 MS.gene76248:intron 20.0%
!!! TTTTTGATTTGGTGTATTTT+TGG - chr1.1:78956248-78956267 MS.gene76248:intron 20.0%
! AAAAAAGGATGAAGAAAAAG+GGG + chr1.1:78955970-78955989 None:intergenic 25.0%
! AATTCGATTTCATTGAAACT+TGG + chr1.1:78956781-78956800 None:intergenic 25.0%
! AGAATTTAAGAAGCTAAAAG+TGG + chr1.1:78956489-78956508 None:intergenic 25.0%
! ATTCGATTTCATTGAAACTT+GGG + chr1.1:78956780-78956799 None:intergenic 25.0%
! CAAGAAATTGGATCTAAAAA+AGG + chr1.1:78955985-78956004 None:intergenic 25.0%
! CAGAAAGAAAAAAAAAGGAA+GGG + chr1.1:78956989-78957008 None:intergenic 25.0%
! CTAAAAAAGGATGAAGAAAA+AGG + chr1.1:78955972-78955991 None:intergenic 25.0%
! TCAAAAAATCATCTCAGAAT+TGG + chr1.1:78956235-78956254 None:intergenic 25.0%
!! ATAATTTGGTACCTTGTTAT+CGG + chr1.1:78956113-78956132 None:intergenic 25.0%
!! TGATTTTTGTGTGTAGAAAT+GGG - chr1.1:78956018-78956037 MS.gene76248:intron 25.0%
!! TTGATTTTTGTGTGTAGAAA+TGG - chr1.1:78956017-78956036 MS.gene76248:intron 25.0%
!!! CTTCCTTTTTTTTTCTTTCT+GGG - chr1.1:78956988-78957007 MS.gene76248:intron 25.0%
!!! CTTTTAGCTTCTTAAATTCT+TGG - chr1.1:78956489-78956508 MS.gene76248:intron 25.0%
!!! GAACATATTTTTGCTTAATG+TGG - chr1.1:78956543-78956562 MS.gene76248:intron 25.0%
!!! GAATTTTGTTTCTTTTACCA+AGG - chr1.1:78956517-78956536 MS.gene76248:intron 25.0%
!!! TCTGAGATGATTTTTTGATT+TGG - chr1.1:78956237-78956256 MS.gene76248:intron 25.0%
!!! TTCCTTTTTTTTTCTTTCTG+GGG - chr1.1:78956989-78957008 MS.gene76248:intron 25.0%
!!! TTTAGTTGATAGCAAAATTG+TGG + chr1.1:78957102-78957121 None:intergenic 25.0%
AAAAAGGATGAAGAAAAAGG+GGG + chr1.1:78955969-78955988 None:intergenic 30.0%
AAATCGAGTGAAACTAGAAA+TGG - chr1.1:78956810-78956829 MS.gene76248:intron 30.0%
AATTCGATTTCACTGTCATA+TGG - chr1.1:78956617-78956636 MS.gene76248:intron 30.0%
AGTTGTGTATAGTGTAAAAG+AGG + chr1.1:78957038-78957057 None:intergenic 30.0%
ATTGGTTACTCTTTCCAAAA+AGG + chr1.1:78956940-78956959 None:intergenic 30.0%
CCAGAAAGAAAAAAAAAGGA+AGG + chr1.1:78956990-78957009 None:intergenic 30.0%
GCAAAAATATGTTCAAACCT+TGG + chr1.1:78956537-78956556 None:intergenic 30.0%
TAGTTAATGACTAAACAGTG+AGG + chr1.1:78956700-78956719 None:intergenic 30.0%
TAGTTTCACTCGATTTCTAA+AGG + chr1.1:78956806-78956825 None:intergenic 30.0%
TTGGTTACTCTTTCCAAAAA+GGG + chr1.1:78956939-78956958 None:intergenic 30.0%
! AAGCAACACACTCTTTTTAA+AGG - chr1.1:78956668-78956687 MS.gene76248:intron 30.0%
! AATTTATGCTGTACCCTTTT+TGG - chr1.1:78956923-78956942 MS.gene76248:intron 30.0%
! AGCAACACACTCTTTTTAAA+GGG - chr1.1:78956669-78956688 MS.gene76248:intron 30.0%
! TCTTTTACACTATACACAAC+TGG - chr1.1:78957037-78957056 MS.gene76248:intron 30.0%
!!! CCTTCCTTTTTTTTTCTTTC+TGG - chr1.1:78956987-78957006 MS.gene76248:intron 30.0%
!!! GTATGCTTTAGCACATTTTT+TGG - chr1.1:78957223-78957242 MS.gene76248:CDS 30.0%
AACTAGAAATGGAGTTGGAA+TGG - chr1.1:78956821-78956840 MS.gene76248:intron 35.0%
AATTTGGTTGCAGTCGTTTA+AGG + chr1.1:78957149-78957168 None:intergenic 35.0%
AGAATATTGGGAGCTACAAA+AGG + chr1.1:78956424-78956443 None:intergenic 35.0%
AGAGTAACCAATGTTAGACT+TGG - chr1.1:78956948-78956967 MS.gene76248:intron 35.0%
AGTGAAACTAGAAATGGAGT+TGG - chr1.1:78956816-78956835 MS.gene76248:intron 35.0%
ATGCCACAAACAATTTGCAA+TGG + chr1.1:78957281-78957300 None:intergenic 35.0%
ATTGTGGAGAGATGAATGTA+AGG + chr1.1:78957086-78957105 None:intergenic 35.0%
ATTTGGTTGCAGTCGTTTAA+GGG + chr1.1:78957148-78957167 None:intergenic 35.0%
CAAAGAAAACGTCTACATTC+AGG - chr1.1:78955933-78955952 MS.gene76248:intron 35.0%
CTCCCCAGAAAGAAAAAAAA+AGG + chr1.1:78956994-78957013 None:intergenic 35.0%
GTTAGTACCAAGTCTAACAT+TGG + chr1.1:78956958-78956977 None:intergenic 35.0%
TACAGATAATGCCGATAACA+AGG - chr1.1:78956099-78956118 MS.gene76248:intron 35.0%
TGTCCATTGCAAATTGTTTG+TGG - chr1.1:78957275-78957294 MS.gene76248:CDS 35.0%
TGTGTATAGTGTAAAAGAGG+TGG + chr1.1:78957035-78957054 None:intergenic 35.0%
TTGTGGAGAGATGAATGTAA+GGG + chr1.1:78957085-78957104 None:intergenic 35.0%
TTGTTATCGGCATTATCTGT+AGG + chr1.1:78956100-78956119 None:intergenic 35.0%
! CCAATTTCTTGCTTCTGAAA+CGG - chr1.1:78955994-78956013 MS.gene76248:intron 35.0%
! CTGAATGTAGACGTTTTCTT+TGG + chr1.1:78955935-78955954 None:intergenic 35.0%
!! CAACACACTCTTTTTAAAGG+GGG - chr1.1:78956671-78956690 MS.gene76248:intron 35.0%
!! GCAACACACTCTTTTTAAAG+GGG - chr1.1:78956670-78956689 MS.gene76248:intron 35.0%
!!! TTTTTTTTCTTTCTGGGGAG+TGG - chr1.1:78956994-78957013 MS.gene76248:intron 35.0%
ATTCAATGAAACACGACCAC+AGG + chr1.1:78956600-78956619 None:intergenic 40.0%
CAAAGGTAAGATTCTTAGCG+AGG - chr1.1:78955900-78955919 MS.gene76248:intron 40.0%
CCGTTTCAGAAGCAAGAAAT+TGG + chr1.1:78955997-78956016 None:intergenic 40.0%
GAAATGGAGTTGGAATGGTA+AGG - chr1.1:78956826-78956845 MS.gene76248:intron 40.0%
TCGATTTCACTGTCATATGG+TGG - chr1.1:78956620-78956639 MS.gene76248:intron 40.0%
TTCCATGTTGCTGTGAATCA+AGG - chr1.1:78957252-78957271 MS.gene76248:CDS 40.0%
TTGTGTGTAGAAATGGGAGA+GGG - chr1.1:78956024-78956043 MS.gene76248:intron 40.0%
TTTGTGTGTAGAAATGGGAG+AGG - chr1.1:78956023-78956042 MS.gene76248:intron 40.0%
! CTACGAACACACTCTTTTGA+AGG - chr1.1:78956721-78956740 MS.gene76248:intron 40.0%
! TCAATGGATTCAACATCTCG+TGG - chr1.1:78955853-78955872 MS.gene76248:CDS 40.0%
!! AAAAGAGTGTGTTGCTTGCT+AGG + chr1.1:78956665-78956684 None:intergenic 40.0%
!! CACTCTTTTGAAGGTGGATT+TGG - chr1.1:78956730-78956749 MS.gene76248:intron 40.0%
!! TTGAAGGTGGATTTGGCTTT+CGG - chr1.1:78956737-78956756 MS.gene76248:intron 40.0%
CACCTTGATTCACAGCAACA+TGG + chr1.1:78957257-78957276 None:intergenic 45.0%
GCTTAATGTGGTATTGCACG+TGG - chr1.1:78956555-78956574 MS.gene76248:intron 45.0%
GTTGGAGAAACAGAAGCTAG+CGG - chr1.1:78956051-78956070 MS.gene76248:intron 45.0%
TGTAGAAATGGGAGAGGGAA+AGG - chr1.1:78956029-78956048 MS.gene76248:intron 45.0%
! ACGTGGTGATGACATGATTC+TGG - chr1.1:78956572-78956591 MS.gene76248:intron 45.0%
!! CGAACACACTCTTTTGAAGG+TGG - chr1.1:78956724-78956743 MS.gene76248:intron 45.0%
!! TGACATGATTCTGGAACCTG+TGG - chr1.1:78956581-78956600 MS.gene76248:intron 45.0%
ACAACTGCGCACCATGGTAA+GGG + chr1.1:78957336-78957355 None:intergenic 50.0%
ACAGAAGCTAGCGGCTGATA+AGG - chr1.1:78956060-78956079 MS.gene76248:intron 50.0%
AGGGATCGTGACTCGTTCAA+TGG - chr1.1:78955837-78955856 MS.gene76248:CDS 50.0%
ATCTGTAGGTTTCTCCGCCT+TGG + chr1.1:78956086-78956105 None:intergenic 50.0%
GAAATGGGAGAGGGAAAGGT+TGG - chr1.1:78956033-78956052 MS.gene76248:intron 50.0%
GCTTCTTCTCGCTTCTTCAG+CGG - chr1.1:78955877-78955896 MS.gene76248:CDS 50.0%
TGGAGTTGGAATGGTAAGGC+AGG - chr1.1:78956830-78956849 MS.gene76248:intron 50.0%
! ATGAGATCAGCACTGAGTCG+AGG - chr1.1:78955817-78955836 MS.gene76248:CDS 50.0%
! TGAGATCAGCACTGAGTCGA+GGG - chr1.1:78955818-78955837 MS.gene76248:CDS 50.0%
AATCATCCCCCTGCTGTGCT+TGG + chr1.1:78957311-78957330 None:intergenic 55.0%
CAACTGCGCACCATGGTAAG+GGG + chr1.1:78957335-78957354 None:intergenic 55.0%
CACAACTGCGCACCATGGTA+AGG + chr1.1:78957337-78957356 None:intergenic 55.0%
GGGATGATTGCCCCTTACCA+TGG - chr1.1:78957322-78957341 MS.gene76248:CDS 55.0%
TCTCGCTTCTTCAGCGGCAA+AGG - chr1.1:78955883-78955902 MS.gene76248:intron 55.0%
AAGCGCACAACTGCGCACCA+TGG + chr1.1:78957342-78957361 None:intergenic 60.0%
AGCGGCTGATAAGGCCAAGG+CGG - chr1.1:78956069-78956088 MS.gene76248:intron 60.0%
ATCCCCCTGCTGTGCTTGGA+AGG + chr1.1:78957307-78957326 None:intergenic 60.0%
ATGCGCCTTCCAAGCACAGC+AGG - chr1.1:78957299-78957318 MS.gene76248:CDS 60.0%
GCTAGCGGCTGATAAGGCCA+AGG - chr1.1:78956066-78956085 MS.gene76248:intron 60.0%
TGCGCCTTCCAAGCACAGCA+GGG - chr1.1:78957300-78957319 MS.gene76248:CDS 60.0%
CGCCTTCCAAGCACAGCAGG+GGG - chr1.1:78957302-78957321 MS.gene76248:CDS 65.0%
GCGCCTTCCAAGCACAGCAG+GGG - chr1.1:78957301-78957320 MS.gene76248:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 78955811 78957363 78955811 ID=MS.gene76248
chr1.1 mRNA 78955811 78957363 78955811 ID=MS.gene76248.t1;Parent=MS.gene76248
chr1.1 exon 78957220 78957363 78957220 ID=MS.gene76248.t1.exon1;Parent=MS.gene76248.t1
chr1.1 CDS 78957220 78957363 78957220 ID=cds.MS.gene76248.t1;Parent=MS.gene76248.t1
chr1.1 exon 78957054 78957140 78957054 ID=MS.gene76248.t1.exon2;Parent=MS.gene76248.t1
chr1.1 CDS 78957054 78957140 78957054 ID=cds.MS.gene76248.t1;Parent=MS.gene76248.t1
chr1.1 exon 78955811 78955900 78955811 ID=MS.gene76248.t1.exon3;Parent=MS.gene76248.t1
chr1.1 CDS 78955811 78955900 78955811 ID=cds.MS.gene76248.t1;Parent=MS.gene76248.t1
Gene Sequence

>MS.gene76248

ATGGCAATGAGATCAGCACTGAGTCGAGGGATCGTGACTCGTTCAATGGATTCAACATCTCGTGGAGCTTCTTCTCGCTTCTTCAGCGGCAAAGGTAAGATTCTTAGCGAGGAAGAACAAGCCAAAGAAAACGTCTACATTCAGGTACTTCATTTCCCCCTTTTTCTTCATCCTTTTTTAGATCCAATTTCTTGCTTCTGAAACGGTTGATTTTTGTGTGTAGAAATGGGAGAGGGAAAGGTTGGAGAAACAGAAGCTAGCGGCTGATAAGGCCAAGGCGGAGAAACCTACAGATAATGCCGATAACAAGGTACCAAATTATTTTGTTTTTAGGTTATTGATTTCAATATTTCTTTCTGCATATTTTGGGTCTGATTAAAACCAGATTTGTGATCTATTAATATTATTATTGTGTTAGTTACCAATTCTGAGATGATTTTTTGATTTGGTGTATTTTTGGATTTGTTACTTTCTGTTTAGTGCAAATTTTAATTTCATTTTTTAGTTTTGAAGCTATTTGAAATTGTATCATATGATGGTTCCTATGATTCATATCTTAAAATTTATTCAATTTTTTTTACGCATTAAGATGTTCAGCTAAATGACAATTCCTTTTGTAGCTCCCAATATTCTGTAACTTTGATATGAATATGATGGATGTTATTTTTTGTTTCACCACTTTTAGCTTCTTAAATTCTTGGACATCGAATTTTGTTTCTTTTACCAAGGTTTGAACATATTTTTGCTTAATGTGGTATTGCACGTGGTGATGACATGATTCTGGAACCTGTGGTCGTGTTTCATTGAATTCGATTTCACTGTCATATGGTGGATTCATTGTTTAGATATTACCTAGCAAGCAACACACTCTTTTTAAAGGGGGAAGCCTCACTGTTTAGTCATTAACTAGCTACGAACACACTCTTTTGAAGGTGGATTTGGCTTTCGGTGAAACTTGTAATTTGTCCCAAGTTTCAATGAAATCGAATTATCCTTTAGAAATCGAGTGAAACTAGAAATGGAGTTGGAATGGTAAGGCAGGAGTAGTAATAAATTATAATAGCATTCTTCAAAACAAACACGTTATTTTGTAATTTGTCACATTTTTTAGAAATTTATGCTGTACCCTTTTTGGAAAGAGTAACCAATGTTAGACTTGGTACTAACTAGCTAGACCCTTCCTTTTTTTTTCTTTCTGGGGAGTGGATTGCAAGTTGTCTACCACCTCTTTTACACTATACACAACTGGAATATTTACAGAATGAGTCAAACCCTTACATTCATCTCTCCACAATTTTGCTATCAACTAAATGCTATGTTTAATGAAATGATATCCCTTAAACGACTGCAACCAAATTCTTCACTTTTAAAATTCTGATGTTTGACACAGATTTTTTCTTGCTAAATATTTTGTATGCTTTAGCACATTTTTTGGTTATAATTCCATGTTGCTGTGAATCAAGGTGTCCATTGCAAATTGTTTGTGGCATGCGCCTTCCAAGCACAGCAGGGGGATGATTGCCCCTTACCATGGTGCGCAGTTGTGCGCTTAA

Protein sequence

>MS.gene76248.t1

MAMRSALSRGIVTRSMDSTSRGASSRFFSGKGKILSEEEQAKENVYIQKWERERLEKQKLAADKAKAEKPTDNADNKVSIANCLWHAPSKHSRGMIAPYHGAQLCA