Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40991.t1 | XP_024633118.1 | 92.9 | 593 | 30 | 3 | 1 | 593 | 1 | 581 | 2.70E-303 | 1051.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40991.t1 | Q8L7K4 | 58.7 | 213 | 81 | 2 | 674 | 886 | 47 | 252 | 2.5e-63 | 245.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40991.t1 | A0A072VIP0 | 92.6 | 593 | 30 | 4 | 1 | 593 | 1 | 579 | 2.0e-300 | 1041.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene40991 | 0.800091 | 1.70E-48 | -1.69E-46 |
| MS.gene049166 | MS.gene40991 | 0.804552 | 2.04E-49 | -1.69E-46 |
| MS.gene04918 | MS.gene40991 | 0.804459 | 2.14E-49 | -1.69E-46 |
| MS.gene04919 | MS.gene40991 | 0.817279 | 3.58E-52 | -1.69E-46 |
| MS.gene04920 | MS.gene40991 | 0.828546 | 8.47E-55 | -1.69E-46 |
| MS.gene049283 | MS.gene40991 | 0.875581 | 2.75E-68 | -1.69E-46 |
| MS.gene049499 | MS.gene40991 | 0.844302 | 8.23E-59 | -1.69E-46 |
| MS.gene049786 | MS.gene40991 | 0.811544 | 6.64E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene40991 | 0.839383 | 1.64E-57 | -1.69E-46 |
| MS.gene050085 | MS.gene40991 | 0.80492 | 1.71E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene40991 | 0.801339 | 9.43E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene40991 | 0.852421 | 4.67E-61 | -1.69E-46 |
| MS.gene050941 | MS.gene40991 | 0.825506 | 4.52E-54 | -1.69E-46 |
| MS.gene050945 | MS.gene40991 | 0.802372 | 5.79E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene40991 | 0.827986 | 1.16E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene40991 | 0.831514 | 1.60E-55 | -1.69E-46 |
| MS.gene051541 | MS.gene40991 | 0.810428 | 1.16E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene40991 | 0.825442 | 4.68E-54 | -1.69E-46 |
| MS.gene051831 | MS.gene40991 | 0.806532 | 7.86E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene40991 | 0.845205 | 4.70E-59 | -1.69E-46 |
| MS.gene052118 | MS.gene40991 | 0.803722 | 3.04E-49 | -1.69E-46 |
| MS.gene052434 | MS.gene40991 | 0.805049 | 1.61E-49 | -1.69E-46 |
| MS.gene052518 | MS.gene40991 | 0.811291 | 7.54E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene40991 | 0.810196 | 1.30E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene40991 | 0.803525 | 3.34E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene40991 | 0.817333 | 3.48E-52 | -1.69E-46 |
| MS.gene053456 | MS.gene40991 | 0.833303 | 5.76E-56 | -1.69E-46 |
| MS.gene053726 | MS.gene40991 | 0.857001 | 2.20E-62 | -1.69E-46 |
| MS.gene053963 | MS.gene40991 | 0.821847 | 3.25E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene40991 | 0.844481 | 7.36E-59 | -1.69E-46 |
| MS.gene054576 | MS.gene40991 | 0.803084 | 4.13E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene40991 | 0.831394 | 1.71E-55 | -1.69E-46 |
| MS.gene054893 | MS.gene40991 | 0.839439 | 1.58E-57 | -1.69E-46 |
| MS.gene054957 | MS.gene40991 | 0.835761 | 1.39E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene40991 | 0.826143 | 3.19E-54 | -1.69E-46 |
| MS.gene055603 | MS.gene40991 | 0.822781 | 1.97E-53 | -1.69E-46 |
| MS.gene056045 | MS.gene40991 | 0.834297 | 3.25E-56 | -1.69E-46 |
| MS.gene056181 | MS.gene40991 | 0.831936 | 1.26E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene40991 | 0.825756 | 3.94E-54 | -1.69E-46 |
| MS.gene056509 | MS.gene40991 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene40991 | 0.817864 | 2.64E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene40991 | 0.834714 | 2.55E-56 | -1.69E-46 |
| MS.gene056787 | MS.gene40991 | 0.819603 | 1.07E-52 | -1.69E-46 |
| MS.gene056831 | MS.gene40991 | 0.862872 | 3.73E-64 | -1.69E-46 |
| MS.gene056849 | MS.gene40991 | 0.817418 | 3.33E-52 | -1.69E-46 |
| MS.gene056901 | MS.gene40991 | 0.810863 | 9.33E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene40991 | 0.83448 | 2.93E-56 | -1.69E-46 |
| MS.gene057102 | MS.gene40991 | 0.812236 | 4.69E-51 | -1.69E-46 |
| MS.gene057155 | MS.gene40991 | 0.844046 | 9.63E-59 | -1.69E-46 |
| MS.gene057208 | MS.gene40991 | 0.823262 | 1.52E-53 | -1.69E-46 |
| MS.gene057622 | MS.gene40991 | 0.836727 | 7.90E-57 | -1.69E-46 |
| MS.gene05781 | MS.gene40991 | 0.827919 | 1.20E-54 | -1.69E-46 |
| MS.gene057890 | MS.gene40991 | -0.814689 | 1.36E-51 | -1.69E-46 |
| MS.gene057893 | MS.gene40991 | -0.802691 | 4.98E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene40991 | 0.849282 | 3.57E-60 | -1.69E-46 |
| MS.gene058429 | MS.gene40991 | 0.82386 | 1.10E-53 | -1.69E-46 |
| MS.gene058592 | MS.gene40991 | 0.826333 | 2.87E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene40991 | 0.812696 | 3.72E-51 | -1.69E-46 |
| MS.gene05878 | MS.gene40991 | 0.807856 | 4.12E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene40991 | 0.839082 | 1.96E-57 | -1.69E-46 |
| MS.gene059170 | MS.gene40991 | 0.81639 | 5.67E-52 | -1.69E-46 |
| MS.gene059283 | MS.gene40991 | 0.801938 | 7.11E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene40991 | 0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene40991 | 0.832193 | 1.09E-55 | -1.69E-46 |
| MS.gene059852 | MS.gene40991 | 0.805391 | 1.37E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene40991 | -0.81898 | 1.48E-52 | -1.69E-46 |
| MS.gene059901 | MS.gene40991 | -0.810609 | 1.06E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene40991 | 0.826597 | 2.49E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene40991 | 0.837757 | 4.31E-57 | -1.69E-46 |
| MS.gene060296 | MS.gene40991 | 0.814918 | 1.21E-51 | -1.69E-46 |
| MS.gene060527 | MS.gene40991 | 0.809454 | 1.88E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene40991 | 0.835556 | 1.57E-56 | -1.69E-46 |
| MS.gene060777 | MS.gene40991 | 0.855312 | 6.86E-62 | -1.69E-46 |
| MS.gene060850 | MS.gene40991 | 0.812628 | 3.85E-51 | -1.69E-46 |
| MS.gene060851 | MS.gene40991 | 0.816724 | 4.77E-52 | -1.69E-46 |
| MS.gene060852 | MS.gene40991 | 0.816536 | 5.26E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene40991 | 0.855806 | 4.93E-62 | -1.69E-46 |
| MS.gene060933 | MS.gene40991 | 0.846557 | 2.02E-59 | -1.69E-46 |
| MS.gene060935 | MS.gene40991 | 0.847895 | 8.65E-60 | -1.69E-46 |
| MS.gene060937 | MS.gene40991 | 0.863771 | 1.96E-64 | -1.69E-46 |
| MS.gene061100 | MS.gene40991 | 0.802028 | 6.81E-49 | -1.69E-46 |
| MS.gene061160 | MS.gene40991 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene06121 | MS.gene40991 | 0.809083 | 2.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40991.t1 | MTR_1g055315 | 92.424 | 594 | 31 | 4 | 1 | 594 | 1 | 580 | 0.0 | 1102 |
| MS.gene40991.t1 | MTR_1g055315 | 91.841 | 527 | 31 | 3 | 68 | 594 | 1 | 515 | 0.0 | 978 |
| MS.gene40991.t1 | MTR_1g055300 | 92.105 | 266 | 21 | 0 | 618 | 883 | 1 | 266 | 4.86e-180 | 521 |
| MS.gene40991.t1 | MTR_7g106640 | 60.934 | 407 | 139 | 5 | 1 | 406 | 1 | 388 | 2.93e-157 | 475 |
| MS.gene40991.t1 | MTR_7g106640 | 60.934 | 407 | 139 | 5 | 1 | 406 | 1 | 388 | 3.41e-156 | 475 |
| MS.gene40991.t1 | MTR_7g106640 | 35.841 | 226 | 128 | 6 | 361 | 576 | 478 | 696 | 2.38e-23 | 106 |
| MS.gene40991.t1 | MTR_1g055300 | 90.830 | 229 | 21 | 0 | 618 | 846 | 1 | 229 | 3.54e-151 | 444 |
| MS.gene40991.t1 | MTR_7g106640 | 59.547 | 309 | 107 | 3 | 98 | 406 | 1 | 291 | 3.06e-116 | 368 |
| MS.gene40991.t1 | MTR_7g106640 | 35.841 | 226 | 128 | 6 | 361 | 576 | 381 | 599 | 2.17e-23 | 106 |
| MS.gene40991.t1 | MTR_7g106640 | 56.067 | 239 | 87 | 3 | 168 | 406 | 1 | 221 | 9.83e-71 | 244 |
| MS.gene40991.t1 | MTR_7g106640 | 35.841 | 226 | 128 | 6 | 361 | 576 | 311 | 529 | 3.37e-23 | 105 |
| MS.gene40991.t1 | MTR_2g066850 | 46.383 | 235 | 100 | 5 | 123 | 356 | 732 | 941 | 8.99e-45 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40991.t1 | AT5G06440 | 45.721 | 444 | 189 | 11 | 1 | 439 | 1 | 397 | 1.00e-110 | 348 |
| MS.gene40991.t1 | AT5G06440 | 45.721 | 444 | 189 | 11 | 1 | 439 | 1 | 397 | 1.00e-110 | 348 |
| MS.gene40991.t1 | AT5G06440 | 57.491 | 287 | 107 | 5 | 1 | 285 | 1 | 274 | 7.24e-108 | 335 |
| MS.gene40991.t1 | AT5G06440 | 44.568 | 451 | 191 | 12 | 1 | 439 | 1 | 404 | 1.43e-106 | 338 |
| MS.gene40991.t1 | AT3G11720 | 40.659 | 455 | 215 | 11 | 1 | 442 | 1 | 413 | 1.09e-105 | 337 |
| MS.gene40991.t1 | AT3G11720 | 40.659 | 455 | 215 | 11 | 1 | 442 | 1 | 413 | 1.09e-105 | 337 |
| MS.gene40991.t1 | AT3G11720 | 40.659 | 455 | 215 | 11 | 1 | 442 | 55 | 467 | 5.45e-105 | 337 |
| MS.gene40991.t1 | AT5G06440 | 46.067 | 356 | 152 | 8 | 87 | 439 | 5 | 323 | 1.98e-89 | 290 |
| MS.gene40991.t1 | AT5G06440 | 45.797 | 345 | 147 | 8 | 98 | 439 | 1 | 308 | 1.11e-85 | 279 |
| MS.gene40991.t1 | AT3G11720 | 41.076 | 353 | 167 | 8 | 98 | 442 | 1 | 320 | 2.14e-84 | 278 |
| MS.gene40991.t1 | AT5G06410 | 58.685 | 213 | 81 | 2 | 674 | 886 | 47 | 252 | 6.21e-82 | 264 |
Find 188 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTCCAATATATCTTTATT+TGG | 0.153676 | 1.3:+40730115 | None:intergenic |
| CTATCAGAGCAGAGTCTTTC+AGG | 0.156087 | 1.3:-40729768 | MS.gene40991:CDS |
| AGCATCATGGGGACCCTTTC+TGG | 0.166923 | 1.3:-40732530 | MS.gene40991:CDS |
| CCAGGTTGGTTCATATTCTT+TGG | 0.225748 | 1.3:-40729730 | MS.gene40991:CDS |
| GTTACCTCTGCTAATAATTC+TGG | 0.226330 | 1.3:+40725104 | None:intergenic |
| TTTGGACAGGGGAGCGATTA+TGG | 0.248683 | 1.3:-40729712 | MS.gene40991:CDS |
| CAGTGTTTGGTTGCTTGTTT+TGG | 0.249795 | 1.3:+40729676 | None:intergenic |
| TGGAATAGATCCAAACTTAA+AGG | 0.262726 | 1.3:-40729820 | MS.gene40991:CDS |
| TTGTAGGACAATAGCGAATA+TGG | 0.275278 | 1.3:-40732188 | MS.gene40991:intron |
| AGCACAATCACCTTAAGTTT+TGG | 0.278051 | 1.3:+40730056 | None:intergenic |
| CATTGTTGAAGATGTAATTA+AGG | 0.282051 | 1.3:+40726970 | None:intergenic |
| GGATTTATTTCCTTTAAGTT+TGG | 0.290070 | 1.3:+40729810 | None:intergenic |
| ATAAGGCCATTTCAATGATT+AGG | 0.293228 | 1.3:-40730279 | MS.gene40991:CDS |
| AATTACGATTATTCTTCATT+TGG | 0.301008 | 1.3:-40726794 | MS.gene40991:CDS |
| TCCTGCAGATTATGGAAATC+AGG | 0.317640 | 1.3:-40724360 | MS.gene40991:intron |
| TATGGCGCCTTCAGGTATTC+TGG | 0.320375 | 1.3:-40729840 | MS.gene40991:intron |
| CTTCTTCAAAGTCCCGCTTT+TGG | 0.322648 | 1.3:+40724105 | None:intergenic |
| ATTTGTTAGTTGAAGCTTGA+TGG | 0.324638 | 1.3:-40725161 | MS.gene40991:intron |
| CTAACTTTCTTGATATGTTA+AGG | 0.325906 | 1.3:-40734208 | MS.gene40991:CDS |
| AATTGTTGTTCTTACGAATT+CGG | 0.343897 | 1.3:-40732670 | MS.gene40991:intron |
| ATTACGATTATTCTTCATTT+GGG | 0.344545 | 1.3:-40726793 | MS.gene40991:CDS |
| TAAGGCCATTTCAATGATTA+GGG | 0.346546 | 1.3:-40730278 | MS.gene40991:CDS |
| CATGCAAGGGGACTCTTCAT+TGG | 0.347603 | 1.3:+40730216 | None:intergenic |
| ATTGCGCCTAATTCCTCGTT+TGG | 0.356072 | 1.3:+40730367 | None:intergenic |
| CCCATGATGCTCTTTGACTC+AGG | 0.369252 | 1.3:+40732542 | None:intergenic |
| TCCCTTTCATACAATGTTAG+TGG | 0.371335 | 1.3:-40733729 | MS.gene40991:CDS |
| GAAGAAGGTTGAAAGAGGTT+TGG | 0.374076 | 1.3:+40726931 | None:intergenic |
| GGTACCAAATAAAGATATAT+TGG | 0.374923 | 1.3:-40730119 | MS.gene40991:CDS |
| AAAGAAACTACATCCTGATT+TGG | 0.379604 | 1.3:-40725718 | MS.gene40991:CDS |
| AGAAGAATTCAGCAAGGCCT+TGG | 0.384310 | 1.3:-40730701 | MS.gene40991:CDS |
| ACGAAAGATTGTGCAGTATA+GGG | 0.388133 | 1.3:-40734727 | MS.gene40991:CDS |
| AATAGCGAATATGGATATAA+AGG | 0.391283 | 1.3:-40732179 | MS.gene40991:CDS |
| CCTTGATAAACTTTATTTCA+AGG | 0.392531 | 1.3:-40732138 | MS.gene40991:CDS |
| CTCACGACATCCTTCACTTC+AGG | 0.396279 | 1.3:+40732488 | None:intergenic |
| CTTCTACCAATAGTTAAAAC+AGG | 0.403760 | 1.3:-40733798 | MS.gene40991:intron |
| CAAGCAACCAAACACTGATT+TGG | 0.407320 | 1.3:-40729670 | MS.gene40991:CDS |
| AGTACTGTTGCATCCATTGT+TGG | 0.410622 | 1.3:+40730481 | None:intergenic |
| GTTTACAGAAGGTTCAGATT+GGG | 0.414761 | 1.3:-40732890 | MS.gene40991:CDS |
| TTTCGTGGCCTCTGTCTTCA+AGG | 0.415130 | 1.3:-40732740 | MS.gene40991:CDS |
| CTAATACATCACAAAATGAT+TGG | 0.417293 | 1.3:-40734160 | MS.gene40991:CDS |
| CGCTGATGCTTTCCAAAAGC+GGG | 0.419435 | 1.3:-40724117 | MS.gene40991:CDS |
| AACGAAAGATTGTGCAGTAT+AGG | 0.423682 | 1.3:-40734728 | MS.gene40991:CDS |
| TTAGGCGCAATTGCACAAAA+AGG | 0.424519 | 1.3:-40730355 | MS.gene40991:CDS |
| GAAGAATTCAGCAAGGCCTT+GGG | 0.429545 | 1.3:-40730700 | MS.gene40991:CDS |
| ATATTCCCTAATCATTGAAA+TGG | 0.431197 | 1.3:+40730273 | None:intergenic |
| CTAGAAAATAGCAAAGCATT+TGG | 0.431782 | 1.3:-40730460 | MS.gene40991:CDS |
| ATCTCAACATTTGTAAGATC+AGG | 0.432552 | 1.3:+40734678 | None:intergenic |
| CATCAGAAAGAAGTTACTTT+CGG | 0.436473 | 1.3:-40732427 | MS.gene40991:intron |
| TTGGTTCATATTCTTTGGAC+AGG | 0.438402 | 1.3:-40729725 | MS.gene40991:CDS |
| GCATCAGAGGTTGCAAATAG+TGG | 0.439065 | 1.3:-40730625 | MS.gene40991:CDS |
| TTTGGTGCATTCAAAATCTC+AGG | 0.443830 | 1.3:-40725700 | MS.gene40991:intron |
| TAGTCATTTGAACAATCAAC+AGG | 0.448097 | 1.3:+40726641 | None:intergenic |
| TGTAACTTACAAACATCAAC+AGG | 0.449928 | 1.3:+40733689 | None:intergenic |
| GGATGTCGTGAGAGTTGATT+TGG | 0.451455 | 1.3:-40732477 | MS.gene40991:CDS |
| GGAGATTGAGGTTTAAGATA+AGG | 0.453740 | 1.3:+40726860 | None:intergenic |
| TGAATACGATACGGTGTAAA+GGG | 0.454165 | 1.3:+40726830 | None:intergenic |
| TGTTTACAGAAGGTTCAGAT+TGG | 0.455542 | 1.3:-40732891 | MS.gene40991:CDS |
| TATCCGCTCGGATGTCAAAC+AGG | 0.456615 | 1.3:-40730317 | MS.gene40991:CDS |
| GTCATTCCAAACGAGGAATT+AGG | 0.457332 | 1.3:-40730373 | MS.gene40991:CDS |
| TGATTTGGAGGGGAAGTACA+AGG | 0.458896 | 1.3:-40725748 | MS.gene40991:CDS |
| CTTTCAACAAGGTATTCAGC+AGG | 0.462678 | 1.3:+40730574 | None:intergenic |
| TCTTGGACCAAATCAGTGTT+TGG | 0.463895 | 1.3:+40729663 | None:intergenic |
| TCATCTTCTAATTCTGATGA+TGG | 0.467744 | 1.3:+40734624 | None:intergenic |
| TTTATTTCAAGGCAGCTCAT+TGG | 0.469289 | 1.3:-40732127 | MS.gene40991:CDS |
| CCTTGAAATAAAGTTTATCA+AGG | 0.469741 | 1.3:+40732138 | None:intergenic |
| ACTCTGGCATCCAAAACTTA+AGG | 0.476785 | 1.3:-40730066 | MS.gene40991:intron |
| TACCTTGTTGAAAGTAAGCC+AGG | 0.477288 | 1.3:-40730565 | MS.gene40991:CDS |
| TGCAGGAGAAAATGCAAAGT+TGG | 0.478471 | 1.3:-40724151 | MS.gene40991:CDS |
| AGGACCTACTGAGGCAAGCC+AGG | 0.483728 | 1.3:-40729748 | MS.gene40991:CDS |
| TGAGATTGTAGAATTAGATA+AGG | 0.486871 | 1.3:-40730437 | MS.gene40991:CDS |
| AATGTTAGTGGAAGGCTTTG+TGG | 0.487941 | 1.3:-40733717 | MS.gene40991:CDS |
| GGAAGAGATTATACAAAGTG+TGG | 0.489400 | 1.3:-40730416 | MS.gene40991:CDS |
| TGTTCGTATAAAGTTGAAGA+AGG | 0.490091 | 1.3:+40726916 | None:intergenic |
| TAAACCTTGTGATATAAATG+AGG | 0.496793 | 1.3:-40730152 | MS.gene40991:CDS |
| ACGCTGAGGAATCGAAGTTA+AGG | 0.496954 | 1.3:+40726889 | None:intergenic |
| TGTCATCTGAAGACATGTCT+TGG | 0.497622 | 1.3:+40729646 | None:intergenic |
| TTCCACTAACATTGTATGAA+AGG | 0.498448 | 1.3:+40733727 | None:intergenic |
| TTATTATACTCGTGTAATTG+AGG | 0.500529 | 1.3:-40724057 | MS.gene40991:CDS |
| CCTGAGTCAAAGAGCATCAT+GGG | 0.500756 | 1.3:-40732542 | MS.gene40991:CDS |
| CTATCAATTTCTTCCATGCA+AGG | 0.506110 | 1.3:+40730202 | None:intergenic |
| ATGAATACGATACGGTGTAA+AGG | 0.506971 | 1.3:+40726829 | None:intergenic |
| GAAATAAAGTTTATCAAGGA+TGG | 0.510256 | 1.3:+40732142 | None:intergenic |
| GGCTATAGAAACATTAGATA+AGG | 0.511449 | 1.3:-40730296 | MS.gene40991:CDS |
| CCCTGAGTCAAAGAGCATCA+TGG | 0.515376 | 1.3:-40732543 | MS.gene40991:CDS |
| TTTCAGGTTTATGTATCTCA+TGG | 0.519297 | 1.3:-40733568 | MS.gene40991:intron |
| CTTCTAATTCTGATGATGGT+AGG | 0.520149 | 1.3:+40734628 | None:intergenic |
| TCCACTAACATTGTATGAAA+GGG | 0.522752 | 1.3:+40733728 | None:intergenic |
| TACGGTGTAAAGGGTGGTGA+TGG | 0.528147 | 1.3:+40726839 | None:intergenic |
| AAGTTGAAGAAGGTTGAAAG+AGG | 0.529141 | 1.3:+40726926 | None:intergenic |
| AACAAGAAAGGTGCTGATTG+AGG | 0.532545 | 1.3:+40726689 | None:intergenic |
| CCTGCAGATTATGGAAATCA+GGG | 0.533197 | 1.3:-40724359 | MS.gene40991:intron |
| TTTCATACAATGTTAGTGGA+AGG | 0.533564 | 1.3:-40733725 | MS.gene40991:CDS |
| TTTACAGAAGGTTCAGATTG+GGG | 0.534340 | 1.3:-40732889 | MS.gene40991:CDS |
| AGACTCTGCTCTGATAGAGT+TGG | 0.537627 | 1.3:+40729774 | None:intergenic |
| AGAGTTAGTTGCTTCTTCAA+CGG | 0.538397 | 1.3:+40724333 | None:intergenic |
| AAGGGTGGTGATGGAGATTG+AGG | 0.538552 | 1.3:+40726848 | None:intergenic |
| CACGACTGGCAGAACAAGAA+AGG | 0.538953 | 1.3:+40726677 | None:intergenic |
| AGGAGTACTTCAGATGATCA+TGG | 0.539089 | 1.3:-40734188 | MS.gene40991:CDS |
| TCAAGGCAGCTCATTGGCAA+TGG | 0.540578 | 1.3:-40732121 | MS.gene40991:CDS |
| ATCAATTTCTTCCATGCAAG+GGG | 0.540811 | 1.3:+40730204 | None:intergenic |
| GTATGAAAGGGAGTTCCCTC+CGG | 0.540998 | 1.3:+40733740 | None:intergenic |
| GTTGAATGCAACGACACGAC+TGG | 0.541819 | 1.3:+40726663 | None:intergenic |
| ATCATGTGAATGTTTACAGA+AGG | 0.541962 | 1.3:-40732901 | MS.gene40991:CDS |
| ACCAAATAAAGATATATTGG+AGG | 0.544818 | 1.3:-40730116 | MS.gene40991:CDS |
| AAGAAAGGATGAAGGTTTCG+TGG | 0.545994 | 1.3:-40732755 | MS.gene40991:intron |
| ATGTAAATTGCTCTTGACAA+AGG | 0.547068 | 1.3:+40725437 | None:intergenic |
| CCATGATGCTCTTTGACTCA+GGG | 0.548096 | 1.3:+40732543 | None:intergenic |
| GGGAGGCTTTGCTTTGCAGA+AGG | 0.548882 | 1.3:-40732453 | MS.gene40991:CDS |
| CGTCATCGTTGAAACCAGAA+AGG | 0.550675 | 1.3:+40732516 | None:intergenic |
| TATCAATTTCTTCCATGCAA+GGG | 0.550940 | 1.3:+40730203 | None:intergenic |
| TAATGACATGTCACCAACAA+TGG | 0.552227 | 1.3:-40730494 | MS.gene40991:CDS |
| TTGTAAGATCAGGTGAGGCA+AGG | 0.553784 | 1.3:+40734688 | None:intergenic |
| TTCGTGGCCTCTGTCTTCAA+GGG | 0.555222 | 1.3:-40732739 | MS.gene40991:CDS |
| GGGAGAGGAAGTATGACGTA+GGG | 0.556684 | 1.3:-40725776 | MS.gene40991:CDS |
| GAAGCACCTTCGACAAACTC+TGG | 0.557116 | 1.3:-40730082 | MS.gene40991:CDS |
| CCTACTGAGGCAAGCCAGGT+TGG | 0.558302 | 1.3:-40729744 | MS.gene40991:CDS |
| TATAAATGAGGTTATTGTTG+AGG | 0.560611 | 1.3:-40730140 | MS.gene40991:CDS |
| ATGACAAAGTCAAGTCAAGA+AGG | 0.562383 | 1.3:-40729629 | MS.gene40991:CDS |
| CCAACCTGGCTTGCCTCAGT+AGG | 0.565505 | 1.3:+40729744 | None:intergenic |
| TGTAAGATCAGGTGAGGCAA+GGG | 0.572242 | 1.3:+40734689 | None:intergenic |
| CACGACTACTAGTGCATCAG+AGG | 0.576208 | 1.3:-40730638 | MS.gene40991:CDS |
| TCATCAGAATTAGAAGATGA+AGG | 0.578946 | 1.3:-40734621 | MS.gene40991:CDS |
| GGTTCATATTCTTTGGACAG+GGG | 0.582356 | 1.3:-40729723 | MS.gene40991:CDS |
| GTCGTGAGAGTTGATTTGGT+GGG | 0.584180 | 1.3:-40732473 | MS.gene40991:CDS |
| TGGATGCCAGAGTTTGTCGA+AGG | 0.584284 | 1.3:+40730076 | None:intergenic |
| GTTGGTTGAAAATTGTGCGC+AGG | 0.587669 | 1.3:+40726734 | None:intergenic |
| TCGCTGATGCTTTCCAAAAG+CGG | 0.587877 | 1.3:-40724118 | MS.gene40991:CDS |
| CCAAAGAATATGAACCAACC+TGG | 0.590279 | 1.3:+40729730 | None:intergenic |
| AGATCCAGAATTATTAGCAG+AGG | 0.590429 | 1.3:-40725108 | MS.gene40991:intron |
| CAATGAAGAGTCCCCTTGCA+TGG | 0.592248 | 1.3:-40730215 | MS.gene40991:CDS |
| TTTGTCAAGAGCAATTTACA+TGG | 0.593720 | 1.3:-40725435 | MS.gene40991:intron |
| TGGTTTCAGACTTTATCAGA+AGG | 0.594007 | 1.3:-40732101 | MS.gene40991:intron |
| TGTTATGTATCGTGAAGGAC+CGG | 0.595211 | 1.3:-40733759 | MS.gene40991:CDS |
| GAATTAGAAGATGAAGGTGG+TGG | 0.595677 | 1.3:-40734615 | MS.gene40991:intron |
| TACCAATAGTTAAAACAGGA+TGG | 0.596011 | 1.3:-40733794 | MS.gene40991:intron |
| TGGTTCATATTCTTTGGACA+GGG | 0.596871 | 1.3:-40729724 | MS.gene40991:CDS |
| CCAAATAAAGATATATTGGA+GGG | 0.596929 | 1.3:-40730115 | MS.gene40991:CDS |
| TTTCAACAAGGTATTCAGCA+GGG | 0.601536 | 1.3:+40730575 | None:intergenic |
| AAGAATTCAGCAAGGCCTTG+GGG | 0.602284 | 1.3:-40730699 | MS.gene40991:CDS |
| AAAGCTAGTCGAGGCCATCT+CGG | 0.603504 | 1.3:+40730240 | None:intergenic |
| GTGAGAGTTGATTTGGTGGG+AGG | 0.607591 | 1.3:-40732470 | MS.gene40991:CDS |
| GAGGGGTATAGTTATCCGCT+CGG | 0.607989 | 1.3:-40730329 | MS.gene40991:CDS |
| TTAGTTGAAGCTTGATGGAG+TGG | 0.608425 | 1.3:-40725156 | MS.gene40991:intron |
| CACCTGGCTTACTTTCAACA+AGG | 0.612895 | 1.3:+40730563 | None:intergenic |
| TGACGCAATTCCTGAAGTGA+AGG | 0.614940 | 1.3:-40732498 | MS.gene40991:CDS |
| TTCCGTGTTATGTATCGTGA+AGG | 0.616474 | 1.3:-40733764 | MS.gene40991:CDS |
| AGATTGTGCAGTATAGGGAG+AGG | 0.617001 | 1.3:-40734722 | MS.gene40991:CDS |
| AGAGATTATACAAAGTGTGG+AGG | 0.618599 | 1.3:-40730413 | MS.gene40991:CDS |
| TTAGTGGAAGGCTTTGTGGA+CGG | 0.620121 | 1.3:-40733713 | MS.gene40991:CDS |
| AACATTTGTAAGATCAGGTG+AGG | 0.620685 | 1.3:+40734683 | None:intergenic |
| TGTCGTGAGAGTTGATTTGG+TGG | 0.621107 | 1.3:-40732474 | MS.gene40991:CDS |
| TATAGATTATGAATACGATA+CGG | 0.621521 | 1.3:+40726821 | None:intergenic |
| AGCGAATATGGATATAAAGG+TGG | 0.622613 | 1.3:-40732176 | MS.gene40991:CDS |
| TTTGAATCAGATTCGCTCTG+AGG | 0.623474 | 1.3:-40724305 | MS.gene40991:intron |
| GCGTGACAATTCCAGCATGT+TGG | 0.624693 | 1.3:+40726716 | None:intergenic |
| ATAACCTCATTTATATCACA+AGG | 0.624766 | 1.3:+40730148 | None:intergenic |
| ATAAAGTTTATCAAGGATGG+AGG | 0.627020 | 1.3:+40732145 | None:intergenic |
| GGGAACAAATATCACTAGTG+AGG | 0.629788 | 1.3:-40732869 | MS.gene40991:intron |
| TTCATTGGCAAAGCTAGTCG+AGG | 0.630398 | 1.3:+40730231 | None:intergenic |
| TTGCGAATTCTAGCATACAA+TGG | 0.633231 | 1.3:+40730673 | None:intergenic |
| ATTGTATGCTAGAATTCGCA+AGG | 0.634051 | 1.3:-40730671 | MS.gene40991:CDS |
| GTACTTCAGATGATCATGGA+AGG | 0.636905 | 1.3:-40734184 | MS.gene40991:CDS |
| GTCATCGTTGAAACCAGAAA+GGG | 0.638872 | 1.3:+40732517 | None:intergenic |
| ATATCGATTACATTCCGAGA+TGG | 0.639455 | 1.3:-40730254 | MS.gene40991:CDS |
| TGGAGGGGAAGTACAAGGAG+TGG | 0.648704 | 1.3:-40725743 | MS.gene40991:CDS |
| TATGTATCGTGAAGGACCGG+AGG | 0.650001 | 1.3:-40733756 | MS.gene40991:CDS |
| GAGAGGAAGTATGACGTAGG+GGG | 0.650683 | 1.3:-40725774 | MS.gene40991:CDS |
| TGATGGAGTGGAAGTTGATG+AGG | 0.658748 | 1.3:-40725144 | MS.gene40991:CDS |
| ACTAACACCATTGTGACTCG+AGG | 0.660623 | 1.3:-40729597 | MS.gene40991:intron |
| AAAGTAGGAATTGTAGACTG+AGG | 0.661096 | 1.3:+40732933 | None:intergenic |
| AGACATACCTCGAGTCACAA+TGG | 0.669331 | 1.3:+40729590 | None:intergenic |
| ATGTATCGTGAAGGACCGGA+GGG | 0.674262 | 1.3:-40733755 | MS.gene40991:CDS |
| AGAATTCAGCAAGGCCTTGG+GGG | 0.674619 | 1.3:-40730698 | MS.gene40991:CDS |
| AGGGAGAGGAAGTATGACGT+AGG | 0.675106 | 1.3:-40725777 | MS.gene40991:CDS |
| AGTAAGTGTCATTCCAAACG+AGG | 0.677677 | 1.3:-40730380 | MS.gene40991:CDS |
| GAGTCTTTCAGGACCTACTG+AGG | 0.685532 | 1.3:-40729757 | MS.gene40991:CDS |
| GGAGAGGAAGTATGACGTAG+GGG | 0.690725 | 1.3:-40725775 | MS.gene40991:CDS |
| ACAGCTTCCCTTGAAGACAG+AGG | 0.696879 | 1.3:+40732732 | None:intergenic |
| ATACGATACGGTGTAAAGGG+TGG | 0.708926 | 1.3:+40726833 | None:intergenic |
| TGAAGAAGCAACTAACTCTG+AGG | 0.709047 | 1.3:-40724329 | MS.gene40991:CDS |
| TCAGAATTAGAAGATGAAGG+TGG | 0.714216 | 1.3:-40734618 | MS.gene40991:CDS |
| TGATAAAGAAGAATTCAGCA+AGG | 0.714264 | 1.3:-40730707 | MS.gene40991:CDS |
| CTTGTTGAAAGTAAGCCAGG+TGG | 0.717879 | 1.3:-40730562 | MS.gene40991:CDS |
| ATTCGTAGAACATAAAACTG+CGG | 0.718959 | 1.3:-40734237 | MS.gene40991:CDS |
| CTGAGTCAAAGAGCATCATG+GGG | 0.725417 | 1.3:-40732541 | MS.gene40991:CDS |
| AGATAAGGTGATACACGCTG+AGG | 0.726900 | 1.3:+40726875 | None:intergenic |
| GTCCTTCACGATACATAACA+CGG | 0.728304 | 1.3:+40733762 | None:intergenic |
| TAGCCTGTTTGACATCCGAG+CGG | 0.766889 | 1.3:+40730314 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 40724037 | 40734760 | 40724037 | ID=MS.gene40991 |
| chr1.3 | mRNA | 40724037 | 40734760 | 40724037 | ID=MS.gene40991.t1;Parent=MS.gene40991 |
| chr1.3 | exon | 40734616 | 40734760 | 40734616 | ID=MS.gene40991.t1.exon1;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40734616 | 40734760 | 40734616 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40734157 | 40734272 | 40734157 | ID=MS.gene40991.t1.exon2;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40734157 | 40734272 | 40734157 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40733699 | 40733807 | 40733699 | ID=MS.gene40991.t1.exon3;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40733699 | 40733807 | 40733699 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40733545 | 40733584 | 40733545 | ID=MS.gene40991.t1.exon4;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40733545 | 40733584 | 40733545 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40732870 | 40732959 | 40732870 | ID=MS.gene40991.t1.exon5;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40732870 | 40732959 | 40732870 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40732671 | 40732770 | 40732671 | ID=MS.gene40991.t1.exon6;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40732671 | 40732770 | 40732671 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40732428 | 40732567 | 40732428 | ID=MS.gene40991.t1.exon7;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40732428 | 40732567 | 40732428 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40732102 | 40732204 | 40732102 | ID=MS.gene40991.t1.exon8;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40732102 | 40732204 | 40732102 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40730067 | 40730752 | 40730067 | ID=MS.gene40991.t1.exon9;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40730067 | 40730752 | 40730067 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40729598 | 40729848 | 40729598 | ID=MS.gene40991.t1.exon10;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40729598 | 40729848 | 40729598 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40728371 | 40728417 | 40728371 | ID=MS.gene40991.t1.exon11;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40728371 | 40728417 | 40728371 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40726623 | 40726996 | 40726623 | ID=MS.gene40991.t1.exon12;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40726623 | 40726996 | 40726623 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40725701 | 40725797 | 40725701 | ID=MS.gene40991.t1.exon13;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40725701 | 40725797 | 40725701 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40725436 | 40725525 | 40725436 | ID=MS.gene40991.t1.exon14;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40725436 | 40725525 | 40725436 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40725109 | 40725174 | 40725109 | ID=MS.gene40991.t1.exon15;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40725109 | 40725174 | 40725109 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40724306 | 40724374 | 40724306 | ID=MS.gene40991.t1.exon16;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40724306 | 40724374 | 40724306 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| chr1.3 | exon | 40724037 | 40724174 | 40724037 | ID=MS.gene40991.t1.exon17;Parent=MS.gene40991.t1 |
| chr1.3 | CDS | 40724037 | 40724174 | 40724037 | ID=cds.MS.gene40991.t1;Parent=MS.gene40991.t1 |
| Gene Sequence |
| Protein sequence |
