AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066112


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066112.t1 MTR_3g108600 96.386 83 3 0 1 83 39 121 5.90e-50 163
MS.gene066112.t1 MTR_1g010120 84.146 82 13 0 2 83 35 116 6.30e-42 142
MS.gene066112.t1 MTR_2g027560 58.537 82 34 0 2 83 37 118 1.23e-25 98.6
MS.gene066112.t1 MTR_4g124440 57.317 82 35 0 2 83 37 118 5.22e-24 94.0
MS.gene066112.t1 MTR_8g092070 54.878 82 37 0 2 83 43 124 4.83e-23 91.3
MS.gene066112.t1 MTR_1g053320 56.098 82 36 0 2 83 39 120 3.18e-22 89.0
MS.gene066112.t1 MTR_7g085390 56.250 80 34 1 2 81 35 113 3.66e-21 85.9
MS.gene066112.t1 MTR_3g095050 54.430 79 35 1 5 83 46 123 3.67e-21 85.9
MS.gene066112.t1 MTR_6g032820 52.381 84 38 2 1 83 34 116 6.76e-20 82.0
MS.gene066112.t1 MTR_4g112025 45.122 82 45 0 2 83 40 121 1.46e-18 78.6
MS.gene066112.t1 MTR_3g031770 50.649 77 38 0 1 77 35 111 4.66e-17 74.3
MS.gene066112.t1 MTR_1g031900 49.351 77 39 0 2 78 33 109 1.43e-16 72.8
MS.gene066112.t1 MTR_1g031900 51.351 74 36 0 2 75 33 106 1.71e-16 72.8
MS.gene066112.t1 MTR_5g078200 48.485 66 34 0 7 72 37 102 4.94e-14 65.9
MS.gene066112.t1 MTR_5g078200 48.485 66 34 0 7 72 4 69 4.97e-14 65.9
MS.gene066112.t1 MTR_3g080410 44.928 69 38 0 1 69 34 102 1.19e-12 61.6
MS.gene066112.t1 MTR_3g065460 43.939 66 37 0 7 72 38 103 1.38e-12 61.6
MS.gene066112.t1 MTR_2g034440 39.706 68 41 0 2 69 31 98 1.71e-12 61.2
MS.gene066112.t1 MTR_5g085580 41.429 70 41 0 3 72 35 104 1.79e-12 61.2
MS.gene066112.t1 MTR_8g085720 43.243 74 42 0 2 75 35 108 2.25e-12 60.8
MS.gene066112.t1 MTR_7g081370 41.772 79 44 1 3 81 38 114 9.20e-12 58.9
MS.gene066112.t1 MTR_7g081350 40.506 79 45 1 3 81 37 113 1.66e-11 58.5
MS.gene066112.t1 MTR_3g083580 37.500 72 45 0 2 73 35 106 2.93e-11 57.8
MS.gene066112.t1 MTR_1g056640 37.143 70 44 0 3 72 48 117 3.88e-11 57.4
MS.gene066112.t1 MTR_1g077990 38.667 75 45 1 1 75 39 112 5.74e-11 57.0
MS.gene066112.t1 MTR_8g012400 37.500 72 45 0 3 74 39 110 6.74e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066112.t1 AT4G31140 69.136 81 25 0 3 83 37 117 9.53e-32 115
MS.gene066112.t1 AT5G58090 65.000 80 28 0 2 81 31 110 1.54e-29 108
MS.gene066112.t1 AT5G20870 60.000 80 32 0 2 81 39 118 1.13e-26 101
MS.gene066112.t1 AT5G64790 53.012 83 39 0 1 83 36 118 2.42e-23 92.0
MS.gene066112.t1 AT3G04010 52.500 80 37 1 2 81 42 120 2.27e-20 83.6
MS.gene066112.t1 AT3G24330 48.780 82 42 0 2 83 48 129 3.78e-20 83.2
MS.gene066112.t1 AT1G64760 53.750 80 36 1 1 80 33 111 3.47e-19 80.5
MS.gene066112.t1 AT1G64760 53.750 80 36 1 1 80 33 111 3.47e-19 80.5
MS.gene066112.t1 AT4G17180 51.899 79 33 1 3 81 35 108 5.35e-19 79.7
MS.gene066112.t1 AT5G58480 48.193 83 42 1 2 84 35 116 6.03e-19 79.7
MS.gene066112.t1 AT2G19440 52.500 80 37 1 1 80 29 107 7.72e-19 79.3
MS.gene066112.t1 AT2G19440 52.500 80 37 1 1 80 29 107 7.72e-19 79.3
MS.gene066112.t1 AT5G18220 55.072 69 30 1 2 70 39 106 2.25e-17 75.1
MS.gene066112.t1 AT2G05790 43.421 76 43 0 4 79 36 111 8.64e-16 70.9
MS.gene066112.t1 AT2G01630 43.662 71 40 0 2 72 32 102 9.40e-15 67.8
MS.gene066112.t1 AT2G01630 43.662 71 40 0 2 72 32 102 9.99e-15 67.8
MS.gene066112.t1 AT2G01630 43.662 71 40 0 2 72 32 102 1.01e-14 67.8
MS.gene066112.t1 AT1G11820 40.845 71 42 0 2 72 53 123 5.90e-14 65.5
MS.gene066112.t1 AT1G11820 40.845 71 42 0 2 72 53 123 7.41e-14 65.1
MS.gene066112.t1 AT1G11820 40.278 72 43 0 1 72 52 123 1.03e-13 64.7
MS.gene066112.t1 AT1G66250 46.970 66 35 0 7 72 46 111 1.88e-13 63.9
MS.gene066112.t1 AT3G13560 36.364 77 49 0 2 78 35 111 1.11e-11 58.9
MS.gene066112.t1 AT3G13560 36.364 77 49 0 2 78 35 111 1.11e-11 58.9
MS.gene066112.t1 AT3G13560 36.364 77 49 0 2 78 35 111 1.11e-11 58.9
MS.gene066112.t1 AT3G13560 36.364 77 49 0 2 78 35 111 1.11e-11 58.9
MS.gene066112.t1 AT3G13560 36.364 77 49 0 2 78 35 111 1.11e-11 58.9
MS.gene066112.t1 AT4G26830 38.806 67 41 0 3 69 35 101 3.81e-11 57.4
MS.gene066112.t1 AT4G26830 38.806 67 41 0 3 69 39 105 3.96e-11 57.4
MS.gene066112.t1 AT5G24318 37.805 82 48 3 3 83 40 119 5.31e-11 57.0
MS.gene066112.t1 AT5G24318 37.805 82 48 3 3 83 40 119 7.39e-11 56.6

Find 13 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGAGCATTTAAGATATT+TGG 0.103415 3.3:+88667361 None:intergenic
GTTTCTGCTCATGTCTCTTC+TGG 0.203329 3.3:-88667241 MS.gene066112:CDS
GTGAAAATGCTGAAAGATAA+TGG 0.333803 3.3:-88667415 MS.gene066112:CDS
GACATGAGCAGAAACATTCT+TGG 0.361876 3.3:+88667249 None:intergenic
CTTCTGGAGGTGTTGACATC+AGG 0.401083 3.3:-88667225 MS.gene066112:intron
AGTGTATAAAGCATGTCATT+TGG 0.415994 3.3:+88667307 None:intergenic
CTGGTATTCAAGTCATGGTT+GGG 0.445896 3.3:-88667333 MS.gene066112:CDS
TGAAAATGCTGAAAGATAAT+GGG 0.469982 3.3:-88667414 MS.gene066112:CDS
TTAAATGCTCTCAAGAAGAC+TGG 0.540820 3.3:-88667352 MS.gene066112:CDS
ACTGGTATTCAAGTCATGGT+TGG 0.565992 3.3:-88667334 MS.gene066112:CDS
TCTGCTCATGTCTCTTCTGG+AGG 0.579116 3.3:-88667238 MS.gene066112:CDS
GAAGACTGGTATTCAAGTCA+TGG 0.586341 3.3:-88667338 MS.gene066112:CDS
GAAAGATAATGGGATTCTGA+AGG 0.642239 3.3:-88667404 MS.gene066112:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATAAATTCAAATAG+AGG + chr3.3:88667433-88667452 None:intergenic 10.0%
!! AAAAAATAAATTCAAATAGA+GGG + chr3.3:88667432-88667451 None:intergenic 10.0%
!!! CTATTTGAATTTATTTTTTT+AGG - chr3.3:88667433-88667452 MS.gene066112:CDS 10.0%
! TGAAAATGCTGAAAGATAAT+GGG - chr3.3:88667213-88667232 MS.gene066112:intron 25.0%
!!! TTGAGAGCATTTAAGATATT+TGG + chr3.3:88667269-88667288 None:intergenic 25.0%
AGTGTATAAAGCATGTCATT+TGG + chr3.3:88667323-88667342 None:intergenic 30.0%
GTGAAAATGCTGAAAGATAA+TGG - chr3.3:88667212-88667231 MS.gene066112:intron 30.0%
TTAAATGCTCTCAAGAAGAC+TGG - chr3.3:88667275-88667294 MS.gene066112:CDS 35.0%
!! GAAAGATAATGGGATTCTGA+AGG - chr3.3:88667223-88667242 MS.gene066112:intron 35.0%
ACTGGTATTCAAGTCATGGT+TGG - chr3.3:88667293-88667312 MS.gene066112:CDS 40.0%
CTGGTATTCAAGTCATGGTT+GGG - chr3.3:88667294-88667313 MS.gene066112:CDS 40.0%
GAAGACTGGTATTCAAGTCA+TGG - chr3.3:88667289-88667308 MS.gene066112:CDS 40.0%
GACATGAGCAGAAACATTCT+TGG + chr3.3:88667381-88667400 None:intergenic 40.0%
TCTTCCAGCTGCTGAAAAAT+GGG - chr3.3:88667352-88667371 MS.gene066112:CDS 40.0%
! TTTCAGCATTTTCACCACTG+TGG + chr3.3:88667207-88667226 None:intergenic 40.0%
GTCTTCCAGCTGCTGAAAAA+TGG - chr3.3:88667351-88667370 MS.gene066112:CDS 45.0%
GTTTCTGCTCATGTCTCTTC+TGG - chr3.3:88667386-88667405 MS.gene066112:CDS 45.0%
! AGCATTTTCACCACTGTGGT+TGG + chr3.3:88667203-88667222 None:intergenic 45.0%
! TCTTTTGTCACCAACCACAG+TGG - chr3.3:88667190-88667209 MS.gene066112:intron 45.0%
!! GAAACCCATTTTTCAGCAGC+TGG + chr3.3:88667359-88667378 None:intergenic 45.0%
!!! TATTTGAATTTATTTTTTTA+GGG - chr3.3:88667434-88667453 MS.gene066112:CDS 5.0%
TCTGCTCATGTCTCTTCTGG+AGG - chr3.3:88667389-88667408 MS.gene066112:CDS 50.0%
!! CTTCTGGAGGTGTTGACATC+AGG - chr3.3:88667402-88667421 MS.gene066112:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 88667182 88667467 88667182 ID=MS.gene066112
chr3.3 mRNA 88667182 88667467 88667182 ID=MS.gene066112.t1;Parent=MS.gene066112
chr3.3 exon 88667226 88667467 88667226 ID=MS.gene066112.t1.exon1;Parent=MS.gene066112.t1
chr3.3 CDS 88667226 88667467 88667226 ID=cds.MS.gene066112.t1;Parent=MS.gene066112.t1
chr3.3 exon 88667182 88667194 88667182 ID=MS.gene066112.t1.exon2;Parent=MS.gene066112.t1
chr3.3 CDS 88667182 88667194 88667182 ID=cds.MS.gene066112.t1;Parent=MS.gene066112.t1
Gene Sequence

>MS.gene066112

TGTACACATCTTTTGTCACCAACCACAGTGGTGAAAATGCTGAAAGATAATGGGATTCTGAAGGTGAAACTGTTTGATGCTGATCCAAATATCTTAAATGCTCTCAAGAAGACTGGTATTCAAGTCATGGTTGGGATTCCAAATGACATGCTTTATACACTTGCTAATAGTCTTCCAGCTGCTGAAAAATGGGTTTCCAAGAATGTTTCTGCTCATGTCTCTTCTGGAGGTGTTGACATCAGGTATACCCTCTATTTGAATTTATTTTTTTAGGGTTTTGTTCTGA

Protein sequence

>MS.gene066112.t1

CTHLLSPTTVVKMLKDNGILKVKLFDADPNILNALKKTGIQVMVGIPNDMLYTLANSLPAAEKWVSKNVSAHVSSGGVDIRVLF