AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069110


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069110.t1 MTR_5g032070 97.110 173 5 0 1 173 1 173 2.98e-122 342
MS.gene069110.t1 MTR_8g471140 59.116 181 57 7 3 173 1 174 1.64e-48 155
MS.gene069110.t1 MTR_5g066330 50.568 176 73 8 1 169 1 169 1.71e-45 147
MS.gene069110.t1 MTR_8g471140 57.317 164 53 7 3 156 1 157 4.18e-38 128
MS.gene069110.t1 MTR_3g100200 38.983 177 94 6 1 169 1 171 5.55e-26 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069110.t1 AT4G38060 44.118 136 57 4 36 171 20 136 1.76e-25 95.5
MS.gene069110.t1 AT4G38060 44.118 136 57 4 36 171 20 136 1.76e-25 95.5
MS.gene069110.t1 AT4G38060 44.118 136 57 4 36 171 20 136 1.76e-25 95.5
MS.gene069110.t1 AT4G38060 40.323 124 55 4 36 159 20 124 3.96e-17 73.6
MS.gene069110.t1 AT5G65480 42.222 90 33 5 83 168 76 150 7.34e-12 60.5

Find 34 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGACACGTTTGTCTTGT+TGG 0.293279 5.4:-22217181 MS.gene069110:CDS
GAGAAAGAGCCTATTGTTAA+TGG 0.294492 5.4:-22217140 MS.gene069110:CDS
GATGACTCAGATTGGTCTAT+TGG 0.319310 5.4:-22216921 MS.gene069110:CDS
TTGGTTATGGAGAGGTAGTA+AGG 0.362202 5.4:-22217162 MS.gene069110:CDS
CACGTTTGTCTTGTTGGTTA+TGG 0.369107 5.4:-22217175 MS.gene069110:CDS
GATGGATTTCCATTAACAAT+AGG 0.374602 5.4:+22217131 None:intergenic
CAAATAATCAAAGGAAGGTT+AGG 0.383783 5.4:-22217046 MS.gene069110:CDS
GGTGGTGGTGGTTGTTTGTC+AGG 0.396455 5.4:-22216954 MS.gene069110:CDS
TTACCAGAAGAAAATTCATT+TGG 0.409882 5.4:+22216744 None:intergenic
TTAGGAAGAAGTGGCAGAGT+AGG 0.411049 5.4:-22217028 MS.gene069110:CDS
ATGACTCAGATTGGTCTATT+GGG 0.420028 5.4:-22216920 MS.gene069110:CDS
TTTGAAGTTTCCTTTGATGA+AGG 0.436540 5.4:-22217072 MS.gene069110:CDS
TAGGAAGAAGTGGCAGAGTA+GGG 0.441023 5.4:-22217027 MS.gene069110:CDS
CCTTCAACTTCCTTGCAACC+TGG 0.459031 5.4:+22216807 None:intergenic
AGTGGCAGAGTAGGGAAGAG+AGG 0.465355 5.4:-22217019 MS.gene069110:CDS
ATGGAAATCCATCTTCTGAA+TGG 0.490855 5.4:-22217121 MS.gene069110:CDS
CCAGGTTGCAAGGAAGTTGA+AGG 0.512670 5.4:-22216807 MS.gene069110:CDS
GTATCATCTGATGGTGGTGG+TGG 0.523059 5.4:-22216966 MS.gene069110:CDS
TTGGTTCCTTGTTATAGACC+AGG 0.523146 5.4:-22216825 MS.gene069110:CDS
CTGAATCTGATGACTCAGAT+TGG 0.527691 5.4:-22216929 MS.gene069110:CDS
TTGAAGTTTCCTTTGATGAA+GGG 0.535589 5.4:-22217071 MS.gene069110:CDS
TGCAGCTGGAAACAATTACA+TGG 0.536763 5.4:-22215487 MS.gene069110:intron
ATTGGGTGGTTAGAGCCTCA+TGG 0.551380 5.4:-22216903 MS.gene069110:CDS
TTATTTGTTCCCTTCATCAA+AGG 0.553314 5.4:+22217062 None:intergenic
AAAGGAAGGTTAGGAAGAAG+TGG 0.568851 5.4:-22217037 MS.gene069110:CDS
TTAGTATCATCTGATGGTGG+TGG 0.573261 5.4:-22216969 MS.gene069110:CDS
TTGTTATAGACCAGGTTGCA+AGG 0.574059 5.4:-22216817 MS.gene069110:CDS
GGGAACAAATAATCAAAGGA+AGG 0.591584 5.4:-22217051 MS.gene069110:CDS
GAAACAATTACATGGAACAA+TGG 0.593678 5.4:-22215479 MS.gene069110:CDS
TGAAGGGAACAAATAATCAA+AGG 0.593937 5.4:-22217055 MS.gene069110:CDS
TTGTCTTGTTGGTTATGGAG+AGG 0.611884 5.4:-22217170 MS.gene069110:CDS
ACTCAGATTGGTCTATTGGG+TGG 0.613034 5.4:-22216917 MS.gene069110:CDS
TTGCAACCTGGTCTATAACA+AGG 0.667827 5.4:+22216819 None:intergenic
CTCTGAAAATCAGAACCATG+AGG 0.713992 5.4:+22216888 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTTTTTTTATTCT+TGG - chr5.4:22216196-22216215 MS.gene069110:intron 10.0%
!! AATAGATATAAAAGATAAAC+TGG - chr5.4:22216733-22216752 MS.gene069110:intron 15.0%
!!! ATATCTATTTCTTATTTTGA+AGG + chr5.4:22216722-22216741 None:intergenic 15.0%
!!! TATCTATTTCTTATTTTGAA+GGG + chr5.4:22216721-22216740 None:intergenic 15.0%
!!! TCATATTTCTTTGTATATTT+TGG - chr5.4:22216477-22216496 MS.gene069110:intron 15.0%
!! ATCATTTCTCAACAATATAA+TGG + chr5.4:22216360-22216379 None:intergenic 20.0%
!! CTTTGTACAAATCAAAATTA+GGG - chr5.4:22217008-22217027 MS.gene069110:CDS 20.0%
!! TCATTTCTCAACAATATAAT+GGG + chr5.4:22216359-22216378 None:intergenic 20.0%
!! TCTTTGTACAAATCAAAATT+AGG - chr5.4:22217007-22217026 MS.gene069110:CDS 20.0%
!!! AACTGTTTTTTTCTTGTTTA+TGG - chr5.4:22216827-22216846 MS.gene069110:CDS 20.0%
!!! ATTTTGCTATATTTGTAGTT+TGG - chr5.4:22216088-22216107 MS.gene069110:intron 20.0%
! AACAAACAGCTCAATTAAAA+TGG + chr5.4:22216988-22217007 None:intergenic 25.0%
! AAGATCTATTAATACTACTC+AGG + chr5.4:22216676-22216695 None:intergenic 25.0%
! AATGTTGAATATAAGCTCTT+TGG - chr5.4:22216625-22216644 MS.gene069110:intron 25.0%
! ATTTCTCAACAATATAATGG+GGG + chr5.4:22216357-22216376 None:intergenic 25.0%
! CAAATTAAGTTCAATTCTCT+TGG + chr5.4:22216928-22216947 None:intergenic 25.0%
! CATTTCTCAACAATATAATG+GGG + chr5.4:22216358-22216377 None:intergenic 25.0%
! TAATCCAAAAACAATAAGCA+TGG + chr5.4:22217122-22217141 None:intergenic 25.0%
! TCCATCATATAAGATGATTT+AGG + chr5.4:22216905-22216924 None:intergenic 25.0%
! TCCCCTAAAAAAAAAATCTT+TGG - chr5.4:22216650-22216669 MS.gene069110:intron 25.0%
! TGAATGTATCAAATTAGTAC+AGG + chr5.4:22217079-22217098 None:intergenic 25.0%
! TTACCAGAAGAAAATTCATT+TGG + chr5.4:22215899-22215918 None:intergenic 25.0%
!! AATTTTCTTCTGGTAAGATT+TGG - chr5.4:22215903-22215922 MS.gene069110:intron 25.0%
!! AGTGGTAATGTAATTAATGA+TGG - chr5.4:22217033-22217052 MS.gene069110:CDS 25.0%
!! TTTGTCATGTATTATGCTTT+TGG + chr5.4:22216166-22216185 None:intergenic 25.0%
!!! AAAGATTTTTTTTTTAGGGG+AGG + chr5.4:22216651-22216670 None:intergenic 25.0%
!!! AGGCCAAAGATTTTTTTTTT+AGG + chr5.4:22216656-22216675 None:intergenic 25.0%
!!! GCCAAAGATTTTTTTTTTAG+GGG + chr5.4:22216654-22216673 None:intergenic 25.0%
!!! GGCCAAAGATTTTTTTTTTA+GGG + chr5.4:22216655-22216674 None:intergenic 25.0%
AAAACAATAAGCATGGATAC+AGG + chr5.4:22217115-22217134 None:intergenic 30.0%
ATATACTTCAAACTGCAAGT+TGG - chr5.4:22216956-22216975 MS.gene069110:CDS 30.0%
CAAATAATCAAAGGAAGGTT+AGG - chr5.4:22215594-22215613 MS.gene069110:intron 30.0%
CAAATCAAAATTAGGGAAAG+TGG - chr5.4:22217015-22217034 MS.gene069110:CDS 30.0%
GAAACAATTACATGGAACAA+TGG - chr5.4:22217161-22217180 MS.gene069110:CDS 30.0%
GCCTAAATCATCTTATATGA+TGG - chr5.4:22216901-22216920 MS.gene069110:CDS 30.0%
GTATAAGTATCTGTGCAAAT+TGG + chr5.4:22216573-22216592 None:intergenic 30.0%
TACAGGAAATAGAGTTAAGA+GGG + chr5.4:22217062-22217081 None:intergenic 30.0%
TATACTTCAAACTGCAAGTT+GGG - chr5.4:22216957-22216976 MS.gene069110:CDS 30.0%
TGAAGGGAACAAATAATCAA+AGG - chr5.4:22215585-22215604 MS.gene069110:intron 30.0%
TTATTTGTTCCCTTCATCAA+AGG + chr5.4:22215581-22215600 None:intergenic 30.0%
TTCAAATGCATCAATCTACT+AGG - chr5.4:22216226-22216245 MS.gene069110:intron 30.0%
TTGAAGTTTCCTTTGATGAA+GGG - chr5.4:22215569-22215588 MS.gene069110:intron 30.0%
! CTACCAAATGAATTTTCTTC+TGG - chr5.4:22215893-22215912 MS.gene069110:intron 30.0%
! GATGGATTTCCATTAACAAT+AGG + chr5.4:22215512-22215531 None:intergenic 30.0%
! GTATCCATGCTTATTGTTTT+TGG - chr5.4:22217115-22217134 MS.gene069110:CDS 30.0%
! TTTGAAGTTTCCTTTGATGA+AGG - chr5.4:22215568-22215587 MS.gene069110:intron 30.0%
!! TCTACTAGGTTTTTGTTTCA+TGG - chr5.4:22216240-22216259 MS.gene069110:intron 30.0%
!!! GTTGTTTTAGTATCATCTGA+TGG - chr5.4:22215665-22215684 MS.gene069110:intron 30.0%
!!! TGATAGTAGTTTTGCTGTTT+TGG - chr5.4:22215796-22215815 MS.gene069110:intron 30.0%
!!! TTCTAGTCTTGTGTGTTTTT+TGG - chr5.4:22216331-22216350 MS.gene069110:intron 30.0%
ACTTACTCTCTCTATTGCTT+TGG - chr5.4:22216439-22216458 MS.gene069110:intron 35.0%
ATGGAAATCCATCTTCTGAA+TGG - chr5.4:22215519-22215538 MS.gene069110:intron 35.0%
CTGTAAGTGCAATGATGATT+AGG - chr5.4:22216122-22216141 MS.gene069110:intron 35.0%
GAAAAACTCCATTCAGAAGA+TGG + chr5.4:22215530-22215549 None:intergenic 35.0%
GAGAAAGAGCCTATTGTTAA+TGG - chr5.4:22215500-22215519 MS.gene069110:intron 35.0%
GGGAACAAATAATCAAAGGA+AGG - chr5.4:22215589-22215608 MS.gene069110:intron 35.0%
GTACAGGAAATAGAGTTAAG+AGG + chr5.4:22217063-22217082 None:intergenic 35.0%
GTGTCTGTCATTATCATCAT+GGG - chr5.4:22216052-22216071 MS.gene069110:intron 35.0%
TGAAACTGTGGATACTATAG+AGG - chr5.4:22216301-22216320 MS.gene069110:intron 35.0%
TGACAACACTAACAACATAG+AGG + chr5.4:22216547-22216566 None:intergenic 35.0%
TGTAACTATGCATGAAACTG+TGG - chr5.4:22216289-22216308 MS.gene069110:intron 35.0%
TGTGTCTGTCATTATCATCA+TGG - chr5.4:22216051-22216070 MS.gene069110:intron 35.0%
TTGCACAGATACTTATACTC+TGG - chr5.4:22216575-22216594 MS.gene069110:intron 35.0%
TTTGAAGGGAACAAATTGCA+GGG + chr5.4:22216707-22216726 None:intergenic 35.0%
TTTGGATTTAGCTACCCAAA+AGG + chr5.4:22216148-22216167 None:intergenic 35.0%
! CAATGATGATTAGGCCTTTT+GGG - chr5.4:22216131-22216150 MS.gene069110:intron 35.0%
! CATTTTTGTTCACAGCTTGT+GGG + chr5.4:22216507-22216526 None:intergenic 35.0%
! TTACTGATTGAATTTGCAGC+TGG - chr5.4:22217139-22217158 MS.gene069110:CDS 35.0%
!! ATGACTCAGATTGGTCTATT+GGG - chr5.4:22215720-22215739 MS.gene069110:intron 35.0%
!! GTTTTAGTATCATCTGATGG+TGG - chr5.4:22215668-22215687 MS.gene069110:intron 35.0%
!! TTTTGAAGGGAACAAATTGC+AGG + chr5.4:22216708-22216727 None:intergenic 35.0%
AAAGGAAGGTTAGGAAGAAG+TGG - chr5.4:22215603-22215622 MS.gene069110:intron 40.0%
CCACAAGCTGTGAACAAAAA+TGG - chr5.4:22216505-22216524 MS.gene069110:intron 40.0%
CTGAATCTGATGACTCAGAT+TGG - chr5.4:22215711-22215730 MS.gene069110:intron 40.0%
CTTTGACACGTTTGTCTTGT+TGG - chr5.4:22215459-22215478 MS.gene069110:CDS 40.0%
GACACACACATTTCTTCATC+AGG + chr5.4:22216037-22216056 None:intergenic 40.0%
TGCAGCTGGAAACAATTACA+TGG - chr5.4:22217153-22217172 MS.gene069110:CDS 40.0%
TTGCAACCTGGTCTATAACA+AGG + chr5.4:22215824-22215843 None:intergenic 40.0%
TTGGTTATGGAGAGGTAGTA+AGG - chr5.4:22215478-22215497 MS.gene069110:CDS 40.0%
TTGTTATAGACCAGGTTGCA+AGG - chr5.4:22215823-22215842 MS.gene069110:intron 40.0%
! CACGTTTGTCTTGTTGGTTA+TGG - chr5.4:22215465-22215484 MS.gene069110:CDS 40.0%
! CTCTGAAAATCAGAACCATG+AGG + chr5.4:22215755-22215774 None:intergenic 40.0%
! GCAATGATGATTAGGCCTTT+TGG - chr5.4:22216130-22216149 MS.gene069110:intron 40.0%
! TTGGTTCCTTGTTATAGACC+AGG - chr5.4:22215815-22215834 MS.gene069110:intron 40.0%
! TTGTCTTGTTGGTTATGGAG+AGG - chr5.4:22215470-22215489 MS.gene069110:CDS 40.0%
!! CCATTTTTGTTCACAGCTTG+TGG + chr5.4:22216508-22216527 None:intergenic 40.0%
!! GATGACTCAGATTGGTCTAT+TGG - chr5.4:22215719-22215738 MS.gene069110:intron 40.0%
!! TTAGTATCATCTGATGGTGG+TGG - chr5.4:22215671-22215690 MS.gene069110:intron 40.0%
TAGGAAGAAGTGGCAGAGTA+GGG - chr5.4:22215613-22215632 MS.gene069110:intron 45.0%
TTAGGAAGAAGTGGCAGAGT+AGG - chr5.4:22215612-22215631 MS.gene069110:intron 45.0%
!! ACTCAGATTGGTCTATTGGG+TGG - chr5.4:22215723-22215742 MS.gene069110:intron 45.0%
ATTGGGTGGTTAGAGCCTCA+TGG - chr5.4:22215737-22215756 MS.gene069110:intron 50.0%
CCAGGTTGCAAGGAAGTTGA+AGG - chr5.4:22215833-22215852 MS.gene069110:intron 50.0%
CCTTCAACTTCCTTGCAACC+TGG + chr5.4:22215836-22215855 None:intergenic 50.0%
!! GTATCATCTGATGGTGGTGG+TGG - chr5.4:22215674-22215693 MS.gene069110:intron 50.0%
AGTGGCAGAGTAGGGAAGAG+AGG - chr5.4:22215621-22215640 MS.gene069110:intron 55.0%
!! GGTGGTGGTGGTTGTTTGTC+AGG - chr5.4:22215686-22215705 MS.gene069110:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 22215449 22217213 22215449 ID=MS.gene069110
chr5.4 mRNA 22215449 22217213 22215449 ID=MS.gene069110.t1;Parent=MS.gene069110
chr5.4 exon 22216748 22217213 22216748 ID=MS.gene069110.t1.exon1;Parent=MS.gene069110.t1
chr5.4 CDS 22216748 22217213 22216748 ID=cds.MS.gene069110.t1;Parent=MS.gene069110.t1
chr5.4 exon 22215449 22215504 22215449 ID=MS.gene069110.t1.exon2;Parent=MS.gene069110.t1
chr5.4 CDS 22215449 22215504 22215449 ID=cds.MS.gene069110.t1;Parent=MS.gene069110.t1
Gene Sequence

>MS.gene069110

ATGGCTGTGTCTTTGACACGTTTGTCTTGTTGGTTATGGAGAGGTAGTAAGGAGAAAGAGCCTATTGTTAATGGAAATCCATCTTCTGAATGGAGTTTTTCATTGAAAGAAAGAGAGTCTTTGAAGTTTCCTTTGATGAAGGGAACAAATAATCAAAGGAAGGTTAGGAAGAAGTGGCAGAGTAGGGAAGAGAGGAGAGTTGATAGAGAGTATGATGTTGTTTTAGTATCATCTGATGGTGGTGGTGGTTGTTTGTCAGGCTCTGAATCTGATGACTCAGATTGGTCTATTGGGTGGTTAGAGCCTCATGGTTCTGATTTTCAGAGTGATGATGATGAGTCTGAATCTGATAGTAGTTTTGCTGTTTTGGTTCCTTGTTATAGACCAGGTTGCAAGGAAGTTGAAGGCTCAAATAATCATCTTTTGAGTGCTATCAAGAAGAATCTACCAAATGAATTTTCTTCTGGTAAGATTTGGAACTTGTTATTGAAATTTGTAATATTTCTGAGCTGATTTACTTTTTACTTAGATGCTTGAATAATCTGTATTAAAATTGATTTGTTTGTTCTTGTTGACGATTTTGATCCTGATGAAGAAATGTGTGTGTCTGTCATTATCATCATGGGTATTGTGTTTGTAATTTTGCTATATTTGTAGTTTGGTAATAATTCAACTGTAAGTGCAATGATGATTAGGCCTTTTGGGTAGCTAAATCCAAAAGCATAATACATGACAAACAATTCTGTTTTTTTGTTTTTTTTTATTCTTGGAATTGTCTTCAAATGCATCAATCTACTAGGTTTTTGTTTCATGGTTTTTATATTTTAATGTTTTAGTGTGTGTAACTATGCATGAAACTGTGGATACTATAGAGGCATGCTATTCTAGTCTTGTGTGTTTTTTGGCCCCCATTATATTGTTGAGAAATGATATTTGAATTGAACAACTATTTTGTGACAACTTTATCTCTTATACACACATTGTGTTTTTACTTACTCTCTCTATTGCTTTGGTTTTTGTGTCAATACTCATATTTCTTTGTATATTTTGGTTGTCCCACAAGCTGTGAACAAAAATGGTTATTCAAATAACACTCCTCTATGTTGTTAGTGTTGTCATGTCCAATTTGCACAGATACTTATACTCTGGTTTTTCGTTACTTATACTCTGTTGAGTAATGTTGAATATAAGCTCTTTGGCCTCCCCTAAAAAAAAAATCTTTGGCCTGAGTAGTATTAATAGATCTTTACTTCGACCCTGCAATTTGTTCCCTTCAAAATAAGAAATAGATATAAAAGATAAACTGGTACTAAATGATATTATGTTAACATGCATGTTAAATAGTTACAGAACAAACTTTTATCTTTTCTAAGCATAGAACTGTTTTTTTCTTGTTTATGGAAAATAAGTTGTCTTTTATATCTTTGTTATTGAATATGAATGTGCGTTCATGCCTAAATCATCTTATATGATGGACCAAGAGAATTGAACTTAATTTGTAACAATTATATACTTCAAACTGCAAGTTGGGTAATTCCATTTTAATTGAGCTGTTTGTTCTTTGTACAAATCAAAATTAGGGAAAGTGGTAATGTAATTAATGATGGCAACCCTCTTAACTCTATTTCCTGTACTAATTTGATACATTCATCTCTATTCTCTACCTGTATCCATGCTTATTGTTTTTGGATTACTGATTGAATTTGCAGCTGGAAACAATTACATGGAACAATGGTTAGCTTCACTTCAGAGTTTTGAAGCGTAG

Protein sequence

>MS.gene069110.t1

MAVSLTRLSCWLWRGSKEKEPIVNGNPSSEWSFSLKERESLKFPLMKGTNNQRKVRKKWQSREERRVDREYDVVLVSSDGGGGCLSGSESDDSDWSIGWLEPHGSDFQSDDDESESDSSFAVLVPCYRPGCKEVEGSNNHLLSAIKKNLPNEFSSAGNNYMEQWLASLQSFEA