AlfalfaGEDB | MS.gene069206

M. sativa cultivar XinJiangDaYe / MS.gene069206

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069206.t1	PNX83819.1	81	42	7	1	23	64	1	41	2.00E-06	61.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069206.t1	A0A2K3LZ62	81.0	42	7	1	23	64	1	41	1.4e-06	61.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene069206	0.806597	7.61E-50	-1.69E-46
MS.gene049821	MS.gene069206	0.816354	5.78E-52	-1.69E-46
MS.gene049825	MS.gene069206	0.825177	5.40E-54	-1.69E-46
MS.gene049873	MS.gene069206	0.816346	5.80E-52	-1.69E-46
MS.gene050277	MS.gene069206	0.809742	1.63E-50	-1.69E-46
MS.gene050303	MS.gene069206	0.861768	8.13E-64	-1.69E-46
MS.gene050807	MS.gene069206	0.805817	1.11E-49	-1.69E-46
MS.gene050815	MS.gene069206	0.804128	2.51E-49	-1.69E-46
MS.gene051328	MS.gene069206	0.823841	1.11E-53	-1.69E-46
MS.gene051700	MS.gene069206	0.807248	5.55E-50	-1.69E-46
MS.gene05188	MS.gene069206	0.84336	1.47E-58	-1.69E-46
MS.gene051975	MS.gene069206	0.806081	9.78E-50	-1.69E-46
MS.gene052012	MS.gene069206	0.815863	7.44E-52	-1.69E-46
MS.gene052309	MS.gene069206	-0.814485	1.50E-51	-1.69E-46
MS.gene052376	MS.gene069206	0.803026	4.24E-49	-1.69E-46
MS.gene052519	MS.gene069206	0.807811	4.21E-50	-1.69E-46
MS.gene052561	MS.gene069206	0.800871	1.18E-48	-1.69E-46
MS.gene052947	MS.gene069206	0.840514	8.31E-58	-1.69E-46
MS.gene053080	MS.gene069206	0.832139	1.12E-55	-1.69E-46
MS.gene053226	MS.gene069206	0.817667	2.93E-52	-1.69E-46
MS.gene054657	MS.gene069206	0.812175	4.84E-51	-1.69E-46
MS.gene054658	MS.gene069206	0.822636	2.13E-53	-1.69E-46
MS.gene05517	MS.gene069206	0.815672	8.20E-52	-1.69E-46
MS.gene055778	MS.gene069206	0.809898	1.51E-50	-1.69E-46
MS.gene055808	MS.gene069206	0.864036	1.62E-64	-1.69E-46
MS.gene055841	MS.gene069206	0.819738	9.92E-53	-1.69E-46
MS.gene056091	MS.gene069206	0.825107	5.61E-54	-1.69E-46
MS.gene056215	MS.gene069206	0.84186	3.68E-58	-1.69E-46
MS.gene056580	MS.gene069206	0.815326	9.79E-52	-1.69E-46
MS.gene056631	MS.gene069206	0.830432	2.95E-55	-1.69E-46
MS.gene05689	MS.gene069206	0.809244	2.08E-50	-1.69E-46
MS.gene056986	MS.gene069206	0.809643	1.71E-50	-1.69E-46
MS.gene057060	MS.gene069206	0.834035	3.78E-56	-1.69E-46
MS.gene057079	MS.gene069206	0.818963	1.49E-52	-1.69E-46
MS.gene057140	MS.gene069206	0.806612	7.56E-50	-1.69E-46
MS.gene057693	MS.gene069206	0.819849	9.36E-53	-1.69E-46
MS.gene057705	MS.gene069206	0.838587	2.63E-57	-1.69E-46
MS.gene058084	MS.gene069206	0.81987	9.25E-53	-1.69E-46
MS.gene058548	MS.gene069206	0.808313	3.29E-50	-1.69E-46
MS.gene058549	MS.gene069206	0.811906	5.54E-51	-1.69E-46
MS.gene05954	MS.gene069206	0.85194	6.40E-61	-1.69E-46
MS.gene059569	MS.gene069206	0.81465	1.38E-51	-1.69E-46
MS.gene05979	MS.gene069206	0.818341	2.06E-52	-1.69E-46
MS.gene059830	MS.gene069206	0.817776	2.77E-52	-1.69E-46
MS.gene059842	MS.gene069206	0.8008	1.22E-48	-1.69E-46
MS.gene06001	MS.gene069206	0.823126	1.64E-53	-1.69E-46
MS.gene060429	MS.gene069206	0.868204	7.77E-66	-1.69E-46
MS.gene060572	MS.gene069206	0.810848	9.40E-51	-1.69E-46
MS.gene060586	MS.gene069206	0.836952	6.92E-57	-1.69E-46
MS.gene060587	MS.gene069206	0.801665	8.09E-49	-1.69E-46
MS.gene060803	MS.gene069206	0.803211	3.88E-49	-1.69E-46
MS.gene060830	MS.gene069206	0.807783	4.27E-50	-1.69E-46
MS.gene060907	MS.gene069206	0.816887	4.39E-52	-1.69E-46
MS.gene060917	MS.gene069206	0.827448	1.56E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTGGTGGAAGTGGATTTA+AGG	0.234381	8.2:-67187436	MS.gene069206:CDS
CCTTGTGCGAGGAGCCCTTT+GGG	0.355356	8.2:-67187729	MS.gene069206:CDS
GAGCTGACAACCTGGTCTTC+AGG	0.360332	8.2:+67187400	None:intergenic
GGAAAATTTGTTTAGGTTGA+TGG	0.363350	8.2:-67187477	MS.gene069206:intron
TCCTCGCACAAGGAATCATT+GGG	0.367121	8.2:+67187739	None:intergenic
CTCCTCGCACAAGGAATCAT+TGG	0.387963	8.2:+67187738	None:intergenic
GCAATGGAGCTCGACAAATA+AGG	0.389446	8.2:-67187354	None:intergenic
TCCTTGTGCGAGGAGCCCTT+TGG	0.431842	8.2:-67187730	MS.gene069206:CDS
AGCTAAAGCTGCGCAGGCAA+TGG	0.436809	8.2:-67187370	MS.gene069206:CDS
TTTGGGACATACTTGTGGTC+AGG	0.447135	8.2:-67187712	MS.gene069206:intron
ATCGAAAGCTAAAGCTGCGC+AGG	0.513460	8.2:-67187376	MS.gene069206:CDS
TGGTGGAAGTGGATTTAAGG+TGG	0.555596	8.2:-67187433	MS.gene069206:CDS
CCCAAAGGGCTCCTCGCACA+AGG	0.565511	8.2:+67187729	None:intergenic
GACCACAAGTATGTCCCAAA+GGG	0.572638	8.2:+67187715	None:intergenic
GGTGGAAGTGGATTTAAGGT+GGG	0.573086	8.2:-67187432	MS.gene069206:CDS
TTTCGATAGAGCTGACAACC+TGG	0.578012	8.2:+67187392	None:intergenic
AGCCCTTTGGGACATACTTG+TGG	0.585503	8.2:-67187717	MS.gene069206:CDS
TTTACGCTTGGCCTCATGTG+CGG	0.593568	8.2:-67187771	None:intergenic
GAAAGAAATACCTGAAGACC+AGG	0.620236	8.2:-67187410	MS.gene069206:CDS
TGACCACAAGTATGTCCCAA+AGG	0.624223	8.2:+67187714	None:intergenic
CCCCAATGATTCCTTGTGCG+AGG	0.625608	8.2:-67187740	MS.gene069206:CDS
CCTCGCACAAGGAATCATTG+GGG	0.735920	8.2:+67187740	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTAGATTTAAACTTTGGA+AGG	-	chr8.2:67187515-67187534	MS.gene069206:intron	20.0%
!!	TGGATATTAGATTTAAACTT+TGG	-	chr8.2:67187511-67187530	MS.gene069206:intron	20.0%
!!!	TTTTGCTGGAAAATTTGTTT+AGG	-	chr8.2:67187628-67187647	MS.gene069206:intron	25.0%
	AGAACACTTTCAATCTAAAG+TGG	-	chr8.2:67187437-67187456	MS.gene069206:CDS	30.0%
!!	AGATTGAAAGTGTTCTGATA+TGG	+	chr8.2:67187433-67187452	None:intergenic	30.0%
!!	GATTGAAAGTGTTCTGATAT+GGG	+	chr8.2:67187432-67187451	None:intergenic	30.0%
!!	GGAAAATTTGTTTAGGTTGA+TGG	-	chr8.2:67187635-67187654	MS.gene069206:intron	30.0%
!!!	TTGTATGGTTTTGTTTTTGC+TGG	-	chr8.2:67187614-67187633	MS.gene069206:intron	30.0%
	ACTGAAAGTTGAGATTAGAG+AGG	-	chr8.2:67187541-67187560	MS.gene069206:intron	35.0%
	CAACCACACACATAATATGT+AGG	+	chr8.2:67187479-67187498	None:intergenic	35.0%
	CTTGAGGATTAATGGTTGTA+TGG	-	chr8.2:67187599-67187618	MS.gene069206:intron	35.0%
	GGTAGTGACAAGAAAAAGAA+GGG	-	chr8.2:67187656-67187675	MS.gene069206:intron	35.0%
	GTGTGTATCTTGAGGATTAA+TGG	-	chr8.2:67187591-67187610	MS.gene069206:intron	35.0%
!	ACACTTTCAATCTAAAGTGG+CGG	-	chr8.2:67187440-67187459	MS.gene069206:CDS	35.0%
!	TGGTAGTGACAAGAAAAAGA+AGG	-	chr8.2:67187655-67187674	MS.gene069206:intron	35.0%
	AGTGACAAGAAAAAGAAGGG+TGG	-	chr8.2:67187659-67187678	MS.gene069206:intron	40.0%
	GAAAGAAATACCTGAAGACC+AGG	-	chr8.2:67187702-67187721	MS.gene069206:intron	40.0%
	GAGTGTTTGTGTGTATCTTG+AGG	-	chr8.2:67187583-67187602	MS.gene069206:intron	40.0%
	GTGCCTACATATTATGTGTG+TGG	-	chr8.2:67187473-67187492	MS.gene069206:intron	40.0%
	TGTGGTTGTGCAATAAAAGC+TGG	-	chr8.2:67187491-67187510	MS.gene069206:intron	40.0%
	ACATACTTGTGGTCAGGTGT+CGG	-	chr8.2:67187406-67187425	MS.gene069206:CDS	45.0%
	CTAAAGTGGCGGTGATTCAT+AGG	-	chr8.2:67187451-67187470	MS.gene069206:CDS	45.0%
	GACAAGAAAAAGAAGGGTGG+TGG	-	chr8.2:67187662-67187681	MS.gene069206:intron	45.0%
	GACCACAAGTATGTCCCAAA+GGG	+	chr8.2:67187400-67187419	None:intergenic	45.0%
	TCCTCGCACAAGGAATCATT+GGG	+	chr8.2:67187376-67187395	None:intergenic	45.0%
	TGACCACAAGTATGTCCCAA+AGG	+	chr8.2:67187401-67187420	None:intergenic	45.0%
	TTTCGATAGAGCTGACAACC+TGG	+	chr8.2:67187723-67187742	None:intergenic	45.0%
!	GGTGGAAGTGGATTTAAGGT+GGG	-	chr8.2:67187680-67187699	MS.gene069206:intron	45.0%
!	TGGTGGAAGTGGATTTAAGG+TGG	-	chr8.2:67187679-67187698	MS.gene069206:intron	45.0%
!	TTTGGGACATACTTGTGGTC+AGG	-	chr8.2:67187400-67187419	MS.gene069206:CDS	45.0%
!!	AAAAAGAAGGGTGGTGGAAG+TGG	-	chr8.2:67187668-67187687	MS.gene069206:intron	45.0%
	ATCGAAAGCTAAAGCTGCGC+AGG	-	chr8.2:67187736-67187755	MS.gene069206:CDS	50.0%
	CCTCGCACAAGGAATCATTG+GGG	+	chr8.2:67187375-67187394	None:intergenic	50.0%
	CTCCTCGCACAAGGAATCAT+TGG	+	chr8.2:67187377-67187396	None:intergenic	50.0%
!!	AGCCCTTTGGGACATACTTG+TGG	-	chr8.2:67187395-67187414	MS.gene069206:CDS	50.0%
!!	GGGTGGTGGAAGTGGATTTA+AGG	-	chr8.2:67187676-67187695	MS.gene069206:intron	50.0%
	AGCTAAAGCTGCGCAGGCAA+TGG	-	chr8.2:67187742-67187761	MS.gene069206:CDS	55.0%
	CCCCAATGATTCCTTGTGCG+AGG	-	chr8.2:67187372-67187391	MS.gene069206:CDS	55.0%
!	GAGCTGACAACCTGGTCTTC+AGG	+	chr8.2:67187715-67187734	None:intergenic	55.0%
	CCTTGTGCGAGGAGCCCTTT+GGG	-	chr8.2:67187383-67187402	MS.gene069206:CDS	60.0%
	TCCTTGTGCGAGGAGCCCTT+TGG	-	chr8.2:67187382-67187401	MS.gene069206:CDS	60.0%
	CCCAAAGGGCTCCTCGCACA+AGG	+	chr8.2:67187386-67187405	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	67187356	67187778	67187356	ID=MS.gene069206
chr8.2	mRNA	67187356	67187778	67187356	ID=MS.gene069206.t1;Parent=MS.gene069206
chr8.2	exon	67187713	67187778	67187713	ID=MS.gene069206.t1.exon1;Parent=MS.gene069206.t1
chr8.2	CDS	67187713	67187778	67187713	ID=cds.MS.gene069206.t1;Parent=MS.gene069206.t1
chr8.2	exon	67187356	67187484	67187356	ID=MS.gene069206.t1.exon2;Parent=MS.gene069206.t1
chr8.2	CDS	67187356	67187484	67187356	ID=cds.MS.gene069206.t1;Parent=MS.gene069206.t1

Gene Sequence

>MS.gene069206

ATGTGCGGTATCACAACCCCAATGATTCCTTGTGCGAGGAGCCCTTTGGGACATACTTGTGGTCAGGTGTCGGCCCATATCAGAACACTTTCAATCTAAAGTGGCGGTGATTCATAGGTGCCTACATATTATGTGTGTGGTTGTGCAATAAAAGCTGGATATTAGATTTAAACTTTGGAAGGAAAACTGAAAGTTGAGATTAGAGAGGTTGAATTGTTGTGTTGTGTGAGTGTTTGTGTGTATCTTGAGGATTAATGGTTGTATGGTTTTGTTTTTGCTGGAAAATTTGTTTAGGTTGATGGTAGTGACAAGAAAAAGAAGGGTGGTGGAAGTGGATTTAAGGTGGGAAAGAAATACCTGAAGACCAGGTTGTCAGCTCTATCGAAAGCTAAAGCTGCGCAGGCAATGGAGCTCGACAAATAA

Protein sequence

>MS.gene069206.t1

MCGITTPMIPCARSPLGHTCGQVDGSDKKKKGGGSGFKVGKKYLKTRLSALSKAKAAQAMELDK