AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070685


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070685.t1 MTR_5g032070 97.688 173 4 0 1 173 1 173 4.77e-123 343
MS.gene070685.t1 MTR_8g471140 59.669 181 56 7 3 173 1 174 2.63e-49 157
MS.gene070685.t1 MTR_5g066330 50.847 177 73 8 1 170 1 170 2.38e-46 149
MS.gene070685.t1 MTR_8g471140 57.317 164 53 7 3 156 1 157 4.32e-38 128
MS.gene070685.t1 MTR_3g100200 39.326 178 94 6 1 170 1 172 1.04e-26 99.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070685.t1 AT4G38060 44.118 136 57 4 36 171 20 136 2.31e-25 95.1
MS.gene070685.t1 AT4G38060 44.118 136 57 4 36 171 20 136 2.31e-25 95.1
MS.gene070685.t1 AT4G38060 44.118 136 57 4 36 171 20 136 2.31e-25 95.1
MS.gene070685.t1 AT4G38060 40.323 124 55 4 36 159 20 124 3.92e-17 73.6
MS.gene070685.t1 AT5G65480 44.048 84 28 5 89 168 82 150 7.57e-12 60.5

Find 34 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGACACGTTTGTCTTGT+TGG 0.293279 5.3:-20688166 MS.gene070685:CDS
GAGAAAGAGCCTATTGTTAA+TGG 0.294492 5.3:-20688125 MS.gene070685:CDS
GATGACTCAGATTGGTCTAT+TGG 0.319310 5.3:-20687906 MS.gene070685:CDS
TTGGTTATGGAGAGGTAGTA+AGG 0.362202 5.3:-20688147 MS.gene070685:CDS
CACGTTTGTCTTGTTGGTTA+TGG 0.369107 5.3:-20688160 MS.gene070685:CDS
GATGGATTTCCATTAACAAT+AGG 0.374602 5.3:+20688116 None:intergenic
CAAATAATCAAAGGAAGGTT+AGG 0.383783 5.3:-20688031 MS.gene070685:CDS
GGTGGTGGTGGTTGTTTGTC+AGG 0.396455 5.3:-20687939 MS.gene070685:CDS
TTACCAGAAGAAAATTCATT+TGG 0.409882 5.3:+20687729 None:intergenic
TTAGGAAGAAGTGGCAGAGT+AGG 0.411049 5.3:-20688013 MS.gene070685:CDS
ATGACTCAGATTGGTCTATT+GGG 0.420028 5.3:-20687905 MS.gene070685:CDS
TTTGAAGTTTCCTTTGATGA+AGG 0.436540 5.3:-20688057 MS.gene070685:CDS
TAGGAAGAAGTGGCAGAGTA+GGG 0.441023 5.3:-20688012 MS.gene070685:CDS
CCTTCAACTTCCTTGCAACC+TGG 0.459031 5.3:+20687792 None:intergenic
AGTGGCAGAGTAGGGAAGAG+AGG 0.465355 5.3:-20688004 MS.gene070685:CDS
ATGGAAATCCATCTTCTGAA+TGG 0.490855 5.3:-20688106 MS.gene070685:CDS
CCAGGTTGCAAGGAAGTTGA+AGG 0.512670 5.3:-20687792 MS.gene070685:CDS
GTATCATCTGATGGTGGTGG+TGG 0.523059 5.3:-20687951 MS.gene070685:CDS
TTGGTTCCTTGTTATAGACC+AGG 0.523146 5.3:-20687810 MS.gene070685:CDS
CTGAATCTGATGACTCAGAT+TGG 0.527691 5.3:-20687914 MS.gene070685:CDS
TTGAAGTTTCCTTTGATGAA+GGG 0.535589 5.3:-20688056 MS.gene070685:CDS
TGCAGCTGGAAACAATTACA+TGG 0.536763 5.3:-20686478 MS.gene070685:intron
ATTGGGTGGTTAGAGCCTCA+TGG 0.551380 5.3:-20687888 MS.gene070685:CDS
TTATTTGTTCCCTTCATCAA+AGG 0.553314 5.3:+20688047 None:intergenic
AAAGGAAGGTTAGGAAGAAG+TGG 0.568851 5.3:-20688022 MS.gene070685:CDS
TTAGTATCATCTGATGGTGG+TGG 0.573261 5.3:-20687954 MS.gene070685:CDS
TTGTTATAGACCAGGTTGCA+AGG 0.574059 5.3:-20687802 MS.gene070685:CDS
GGGAACAAATAATCAAAGGA+AGG 0.591584 5.3:-20688036 MS.gene070685:CDS
GAAACAATTACATGGAACAA+TGG 0.593678 5.3:-20686470 MS.gene070685:CDS
TGAAGGGAACAAATAATCAA+AGG 0.593937 5.3:-20688040 MS.gene070685:CDS
TTGTCTTGTTGGTTATGGAG+AGG 0.611884 5.3:-20688155 MS.gene070685:CDS
ACTCAGATTGGTCTATTGGG+TGG 0.613034 5.3:-20687902 MS.gene070685:CDS
TTGCAACCTGGTCTATAACA+AGG 0.667827 5.3:+20687804 None:intergenic
CTCTGAAAATCAGAACCATG+AGG 0.713992 5.3:+20687873 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTTTTTTTATTCT+TGG - chr5.3:20687187-20687206 MS.gene070685:intron 10.0%
!! AATAGATATAAAAGATAAAC+TGG - chr5.3:20687724-20687743 MS.gene070685:intron 15.0%
!!! ATATCTATTTCTTATTTTGA+AGG + chr5.3:20687713-20687732 None:intergenic 15.0%
!!! TATCTATTTCTTATTTTGAA+GGG + chr5.3:20687712-20687731 None:intergenic 15.0%
!!! TCATATTTCTTTGTATATTT+TGG - chr5.3:20687468-20687487 MS.gene070685:intron 15.0%
!! ATCATTTCTCAACAATATAA+TGG + chr5.3:20687351-20687370 None:intergenic 20.0%
!! CTTTGTACAAATCAAAATTA+GGG - chr5.3:20688000-20688019 MS.gene070685:CDS 20.0%
!! TCATTTCTCAACAATATAAT+GGG + chr5.3:20687350-20687369 None:intergenic 20.0%
!! TCTTTGTACAAATCAAAATT+AGG - chr5.3:20687999-20688018 MS.gene070685:CDS 20.0%
!!! ACTGTTTTTTTTCTTGTTTA+TGG - chr5.3:20687819-20687838 MS.gene070685:CDS 20.0%
!!! ATTTTGCTATATTTGTAGTT+TGG - chr5.3:20687079-20687098 MS.gene070685:intron 20.0%
! AACAAACAGCTCAATTAAAA+TGG + chr5.3:20687980-20687999 None:intergenic 25.0%
! AAGATCTATTAATACTACTC+AGG + chr5.3:20687667-20687686 None:intergenic 25.0%
! AATGTTGAATATAAGCTCTT+TGG - chr5.3:20687616-20687635 MS.gene070685:intron 25.0%
! ATTTCTCAACAATATAATGG+GGG + chr5.3:20687348-20687367 None:intergenic 25.0%
! CAAATTAAGTTCAATTCTCT+TGG + chr5.3:20687920-20687939 None:intergenic 25.0%
! CATTTCTCAACAATATAATG+GGG + chr5.3:20687349-20687368 None:intergenic 25.0%
! GCTTAAATCATCTTATATGA+TGG - chr5.3:20687893-20687912 MS.gene070685:CDS 25.0%
! TATTCCAAAAACAATAAGCA+TGG + chr5.3:20688107-20688126 None:intergenic 25.0%
! TCCCCTAAAAAAAAAATCTT+TGG - chr5.3:20687641-20687660 MS.gene070685:intron 25.0%
! TGAATGTATCAAATTAGTAC+AGG + chr5.3:20688071-20688090 None:intergenic 25.0%
! TTACCAGAAGAAAATTCATT+TGG + chr5.3:20686890-20686909 None:intergenic 25.0%
!! AATTTTCTTCTGGTAAGATT+TGG - chr5.3:20686894-20686913 MS.gene070685:intron 25.0%
!! AGTGGTAATGTAATTAATGA+TGG - chr5.3:20688025-20688044 MS.gene070685:CDS 25.0%
!! TTTGTCATGTATTATGCTTT+TGG + chr5.3:20687157-20687176 None:intergenic 25.0%
!!! AAAGATTTTTTTTTTAGGGG+AGG + chr5.3:20687642-20687661 None:intergenic 25.0%
!!! AGGCCAAAGATTTTTTTTTT+AGG + chr5.3:20687647-20687666 None:intergenic 25.0%
!!! GCCAAAGATTTTTTTTTTAG+GGG + chr5.3:20687645-20687664 None:intergenic 25.0%
!!! GGCCAAAGATTTTTTTTTTA+GGG + chr5.3:20687646-20687665 None:intergenic 25.0%
AAAACAATAAGCATGGATAC+AGG + chr5.3:20688100-20688119 None:intergenic 30.0%
ATATACTTCAAACTGCAAGT+TGG - chr5.3:20687948-20687967 MS.gene070685:CDS 30.0%
CAAATAATCAAAGGAAGGTT+AGG - chr5.3:20686585-20686604 MS.gene070685:intron 30.0%
CAAATCAAAATTAGGGAAAG+TGG - chr5.3:20688007-20688026 MS.gene070685:CDS 30.0%
GAAACAATTACATGGAACAA+TGG - chr5.3:20688146-20688165 MS.gene070685:CDS 30.0%
GTATAAGTATCTGTGCAAAT+TGG + chr5.3:20687564-20687583 None:intergenic 30.0%
TACAGGAAATAGAGTTAAGA+GGG + chr5.3:20688054-20688073 None:intergenic 30.0%
TATACTTCAAACTGCAAGTT+GGG - chr5.3:20687949-20687968 MS.gene070685:CDS 30.0%
TGAAGGGAACAAATAATCAA+AGG - chr5.3:20686576-20686595 MS.gene070685:intron 30.0%
TTATTTGTTCCCTTCATCAA+AGG + chr5.3:20686572-20686591 None:intergenic 30.0%
TTCAAATGCATCAATCTACT+AGG - chr5.3:20687217-20687236 MS.gene070685:intron 30.0%
TTGAAGTTTCCTTTGATGAA+GGG - chr5.3:20686560-20686579 MS.gene070685:intron 30.0%
! CTACCAAATGAATTTTCTTC+TGG - chr5.3:20686884-20686903 MS.gene070685:intron 30.0%
! GATGGATTTCCATTAACAAT+AGG + chr5.3:20686503-20686522 None:intergenic 30.0%
! GTATCCATGCTTATTGTTTT+TGG - chr5.3:20688100-20688119 MS.gene070685:CDS 30.0%
! TTTGAAGTTTCCTTTGATGA+AGG - chr5.3:20686559-20686578 MS.gene070685:intron 30.0%
!! TCTACTAGGTTTTTGTTTCA+TGG - chr5.3:20687231-20687250 MS.gene070685:intron 30.0%
!!! GTTGTTTTAGTATCATCTGA+TGG - chr5.3:20686656-20686675 MS.gene070685:intron 30.0%
!!! TGATAGTAGTTTTGCTGTTT+TGG - chr5.3:20686787-20686806 MS.gene070685:intron 30.0%
!!! TTCTAGTCTTGTGTGTTTTT+TGG - chr5.3:20687322-20687341 MS.gene070685:intron 30.0%
ACTTACTCTCTCTATTGCTT+TGG - chr5.3:20687430-20687449 MS.gene070685:intron 35.0%
ATGGAAATCCATCTTCTGAA+TGG - chr5.3:20686510-20686529 MS.gene070685:intron 35.0%
CTGTAAGTGCAATGATGATT+AGG - chr5.3:20687113-20687132 MS.gene070685:intron 35.0%
GAAAAACTCCATTCAGAAGA+TGG + chr5.3:20686521-20686540 None:intergenic 35.0%
GAGAAAGAGCCTATTGTTAA+TGG - chr5.3:20686491-20686510 MS.gene070685:intron 35.0%
GGGAACAAATAATCAAAGGA+AGG - chr5.3:20686580-20686599 MS.gene070685:intron 35.0%
GTACAGGAAATAGAGTTAAG+AGG + chr5.3:20688055-20688074 None:intergenic 35.0%
GTGTCTGTCATTATCATCAT+GGG - chr5.3:20687043-20687062 MS.gene070685:intron 35.0%
TGAAACTGTGGATACTATAG+AGG - chr5.3:20687292-20687311 MS.gene070685:intron 35.0%
TGACAACACTAACAACATAG+AGG + chr5.3:20687538-20687557 None:intergenic 35.0%
TGTAACTATGCATGAAACTG+TGG - chr5.3:20687280-20687299 MS.gene070685:intron 35.0%
TGTGTCTGTCATTATCATCA+TGG - chr5.3:20687042-20687061 MS.gene070685:intron 35.0%
TTGCACAGATACTTATACTC+TGG - chr5.3:20687566-20687585 MS.gene070685:intron 35.0%
TTTGAAGGGAACAAATTGCA+GGG + chr5.3:20687698-20687717 None:intergenic 35.0%
TTTGGATTTAGCTACCCAAA+AGG + chr5.3:20687139-20687158 None:intergenic 35.0%
! ATACTGATTGAATTTGCAGC+TGG - chr5.3:20688124-20688143 MS.gene070685:CDS 35.0%
! CAATGATGATTAGGCCTTTT+GGG - chr5.3:20687122-20687141 MS.gene070685:intron 35.0%
! CATTTTTGTTCACAGCTTGT+GGG + chr5.3:20687498-20687517 None:intergenic 35.0%
!! ATGACTCAGATTGGTCTATT+GGG - chr5.3:20686711-20686730 MS.gene070685:intron 35.0%
!! GTTTTAGTATCATCTGATGG+TGG - chr5.3:20686659-20686678 MS.gene070685:intron 35.0%
!! TTTTGAAGGGAACAAATTGC+AGG + chr5.3:20687699-20687718 None:intergenic 35.0%
AAAGGAAGGTTAGGAAGAAG+TGG - chr5.3:20686594-20686613 MS.gene070685:intron 40.0%
CCACAAGCTGTGAACAAAAA+TGG - chr5.3:20687496-20687515 MS.gene070685:intron 40.0%
CTGAATCTGATGACTCAGAT+TGG - chr5.3:20686702-20686721 MS.gene070685:intron 40.0%
CTTTGACACGTTTGTCTTGT+TGG - chr5.3:20686450-20686469 MS.gene070685:CDS 40.0%
GACACACACATTTCTTCATC+AGG + chr5.3:20687028-20687047 None:intergenic 40.0%
TGCAGCTGGAAACAATTACA+TGG - chr5.3:20688138-20688157 MS.gene070685:CDS 40.0%
TTGCAACCTGGTCTATAACA+AGG + chr5.3:20686815-20686834 None:intergenic 40.0%
TTGGTTATGGAGAGGTAGTA+AGG - chr5.3:20686469-20686488 MS.gene070685:CDS 40.0%
TTGTTATAGACCAGGTTGCA+AGG - chr5.3:20686814-20686833 MS.gene070685:intron 40.0%
! CACGTTTGTCTTGTTGGTTA+TGG - chr5.3:20686456-20686475 MS.gene070685:CDS 40.0%
! CTCTGAAAATCAGAACCATG+AGG + chr5.3:20686746-20686765 None:intergenic 40.0%
! GCAATGATGATTAGGCCTTT+TGG - chr5.3:20687121-20687140 MS.gene070685:intron 40.0%
! TTGGTTCCTTGTTATAGACC+AGG - chr5.3:20686806-20686825 MS.gene070685:intron 40.0%
! TTGTCTTGTTGGTTATGGAG+AGG - chr5.3:20686461-20686480 MS.gene070685:CDS 40.0%
!! CCATTTTTGTTCACAGCTTG+TGG + chr5.3:20687499-20687518 None:intergenic 40.0%
!! GATGACTCAGATTGGTCTAT+TGG - chr5.3:20686710-20686729 MS.gene070685:intron 40.0%
!! TTAGTATCATCTGATGGTGG+TGG - chr5.3:20686662-20686681 MS.gene070685:intron 40.0%
TAGGAAGAAGTGGCAGAGTA+GGG - chr5.3:20686604-20686623 MS.gene070685:intron 45.0%
TTAGGAAGAAGTGGCAGAGT+AGG - chr5.3:20686603-20686622 MS.gene070685:intron 45.0%
!! ACTCAGATTGGTCTATTGGG+TGG - chr5.3:20686714-20686733 MS.gene070685:intron 45.0%
ATTGGGTGGTTAGAGCCTCA+TGG - chr5.3:20686728-20686747 MS.gene070685:intron 50.0%
CCAGGTTGCAAGGAAGTTGA+AGG - chr5.3:20686824-20686843 MS.gene070685:intron 50.0%
CCTTCAACTTCCTTGCAACC+TGG + chr5.3:20686827-20686846 None:intergenic 50.0%
!! GTATCATCTGATGGTGGTGG+TGG - chr5.3:20686665-20686684 MS.gene070685:intron 50.0%
AGTGGCAGAGTAGGGAAGAG+AGG - chr5.3:20686612-20686631 MS.gene070685:intron 55.0%
!! GGTGGTGGTGGTTGTTTGTC+AGG - chr5.3:20686677-20686696 MS.gene070685:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 20686440 20688198 20686440 ID=MS.gene070685
chr5.3 mRNA 20686440 20688198 20686440 ID=MS.gene070685.t1;Parent=MS.gene070685
chr5.3 exon 20687733 20688198 20687733 ID=MS.gene070685.t1.exon1;Parent=MS.gene070685.t1
chr5.3 CDS 20687733 20688198 20687733 ID=cds.MS.gene070685.t1;Parent=MS.gene070685.t1
chr5.3 exon 20686440 20686495 20686440 ID=MS.gene070685.t1.exon2;Parent=MS.gene070685.t1
chr5.3 CDS 20686440 20686495 20686440 ID=cds.MS.gene070685.t1;Parent=MS.gene070685.t1
Gene Sequence

>MS.gene070685

ATGGCTGTGTCTTTGACACGTTTGTCTTGTTGGTTATGGAGAGGTAGTAAGGAGAAAGAGCCTATTGTTAATGGAAATCCATCTTCTGAATGGAGTTTTTCATTGAAAGAAAGAGAGTCTTTGAAGTTTCCTTTGATGAAGGGAACAAATAATCAAAGGAAGGTTAGGAAGAAGTGGCAGAGTAGGGAAGAGAGGAGAGTTGATAGAGAGTATGATGTTGTTTTAGTATCATCTGATGGTGGTGGTGGTTGTTTGTCAGGCTCTGAATCTGATGACTCAGATTGGTCTATTGGGTGGTTAGAGCCTCATGGTTCTGATTTTCAGAGTGATGATGATGAGTCTGAATCTGATAGTAGTTTTGCTGTTTTGGTTCCTTGTTATAGACCAGGTTGCAAGGAAGTTGAAGGCTCAAATAATCATCTTTTGAGTGCTATCAAGAAGAATCTACCAAATGAATTTTCTTCTGGTAAGATTTGGAACTTGTTATTGAAATTTGTAATATTTCTGAGCTGATTTACTTTTTACTTAGATGCTTGAATAATCTGTATTAAAATTGATTTGTTTGTTCTTGTTGACGATTTTGATCCTGATGAAGAAATGTGTGTGTCTGTCATTATCATCATGGGTATTGTGTTTGTAATTTTGCTATATTTGTAGTTTGGTAATAATTCAACTGTAAGTGCAATGATGATTAGGCCTTTTGGGTAGCTAAATCCAAAAGCATAATACATGACAAACAATTCTGTTTTTTTGTTTTTTTTTATTCTTGGAATTGTCTTCAAATGCATCAATCTACTAGGTTTTTGTTTCATGGTTTTTATATTTTAATGTTTTAGTGTGTGTAACTATGCATGAAACTGTGGATACTATAGAGGCATGCTATTCTAGTCTTGTGTGTTTTTTGGCCCCCATTATATTGTTGAGAAATGATATTTGAATTGAACAACTATTTTGTGACAACTTTATCTCTTATACACACATTGTGTTTTTACTTACTCTCTCTATTGCTTTGGTTTTTGTGTCAATACTCATATTTCTTTGTATATTTTGGTTGTCCCACAAGCTGTGAACAAAAATGGTTATTCAAATAACACTCCTCTATGTTGTTAGTGTTGTCATGTCCAATTTGCACAGATACTTATACTCTGGTTTTTCGTTACTTATACTCTGTTGAGTAATGTTGAATATAAGCTCTTTGGCCTCCCCTAAAAAAAAAATCTTTGGCCTGAGTAGTATTAATAGATCTTTACTTCGACCCTGCAATTTGTTCCCTTCAAAATAAGAAATAGATATAAAAGATAAACTGGTACTAAATGATATTATGTTAACATGCATGTTAAATAGTTACAGAACAAACTTTTATCTTTTCTAAGCATAGAACTGTTTTTTTTCTTGTTTATGGAAAATAAGTTGTCTTTTATATCTTTATGATTGAATATGAATGTGCGTTCATGCTTAAATCATCTTATATGATGGACCAAGAGAATTGAACTTAATTTGTAACAATTATATACTTCAAACTGCAAGTTGGGTAATTCCATTTTAATTGAGCTGTTTGTTCTTTGTACAAATCAAAATTAGGGAAAGTGGTAATGTAATTAATGATGGCAACCCTCTTAACTCTATTTCCTGTACTAATTTGATACATTCATCTCTACCTGTATCCATGCTTATTGTTTTTGGAATACTGATTGAATTTGCAGCTGGAAACAATTACATGGAACAATGGTTAGCTTCACTTCAGAATTTTGAAGCGTAG

Protein sequence

>MS.gene070685.t1

MAVSLTRLSCWLWRGSKEKEPIVNGNPSSEWSFSLKERESLKFPLMKGTNNQRKVRKKWQSREERRVDREYDVVLVSSDGGGGCLSGSESDDSDWSIGWLEPHGSDFQSDDDESESDSSFAVLVPCYRPGCKEVEGSNNHLLSAIKKNLPNEFSSAGNNYMEQWLASLQNFEA