AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24266


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24266.t1 MTR_1g022355 95.000 280 11 1 1 280 90 366 0.0 532
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24266.t1 AT1G19520 56.631 279 100 5 1 274 108 370 5.32e-87 264
MS.gene24266.t1 AT1G19520 56.429 280 101 5 1 275 108 371 6.55e-84 266

Find 71 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGTTCAAGATTTA+TGG 0.133676 1.1:-1929319 MS.gene24266:CDS
TCAGCCACTTGTTCCCATTT+AGG 0.207802 1.1:+1929280 None:intergenic
TTGATCTATCGGATCCTAAA+TGG 0.216797 1.1:-1929294 MS.gene24266:CDS
ATGAGGCGGGAGAAGTTATT+TGG 0.238365 1.1:-1929249 MS.gene24266:CDS
TTAGAGAGCTTAGGTTTAAA+TGG 0.238681 1.1:-1929525 MS.gene24266:CDS
GGAGGTTAGTAATAAGGTTT+TGG 0.281718 1.1:-1929659 MS.gene24266:CDS
TGTTTGCATTTCCCGGTTAT+CGG 0.314823 1.1:+1929211 None:intergenic
GGTAACTTGTTTGCATTTCC+CGG 0.333463 1.1:+1929204 None:intergenic
GAATTATCCTTTATACTTCA+AGG 0.334354 1.1:-1929053 MS.gene24266:CDS
AAGGAGGCGAAGCCGATAAC+CGG 0.340946 1.1:-1929223 MS.gene24266:CDS
TGTGTGGGAGGTTAGTAATA+AGG 0.359950 1.1:-1929665 MS.gene24266:CDS
TAGAGCTTCTACAGCCTACT+AGG 0.360591 1.1:-1929114 MS.gene24266:CDS
TAAATGGGCACGTGAGAAAA+TGG 0.380143 1.1:-1929509 MS.gene24266:CDS
TGCATCTTCGCGATTTGATT+CGG 0.380839 1.1:-1929716 MS.gene24266:CDS
ATGGAAAAGGGTAGTGGTAG+TGG 0.392895 1.1:-1929757 MS.gene24266:CDS
ACAGTTACCTTGAAGTATAA+AGG 0.399658 1.1:+1929046 None:intergenic
TGTAGATTTGCTGGTGAAGT+TGG 0.408581 1.1:-1929565 MS.gene24266:CDS
GATGCTGGTGTTCGTGTTGA+TGG 0.410669 1.1:-1929388 MS.gene24266:CDS
AAATTAAGTTCAAGATTTAT+GGG 0.422169 1.1:-1929318 MS.gene24266:CDS
CGAGAAAATTCTGTCGTTGA+AGG 0.433246 1.1:-1929182 MS.gene24266:CDS
AAAGAGATGTGTAGATTTGC+TGG 0.447104 1.1:-1929574 MS.gene24266:CDS
GTTGACCCTGGTAGCTGAAT+GGG 0.449689 1.1:-1929137 MS.gene24266:CDS
ATGAGGATTTGGAGAAAATG+AGG 0.450985 1.1:-1929471 MS.gene24266:CDS
TAGAGAGCTTAGGTTTAAAT+GGG 0.451572 1.1:-1929524 MS.gene24266:CDS
TGATCTATCGGATCCTAAAT+GGG 0.452985 1.1:-1929293 MS.gene24266:CDS
GATTTATGGGCTTGATCTAT+CGG 0.456068 1.1:-1929305 MS.gene24266:CDS
TGGAAAAGGGTAGTGGTAGT+GGG 0.463563 1.1:-1929756 MS.gene24266:CDS
GTTGAGGAGAAGAAGGTTGT+TGG 0.471339 1.1:-1929361 MS.gene24266:CDS
TTGATCCAATCCACCCTAGT+AGG 0.474961 1.1:+1929100 None:intergenic
GCTCTACCCATTCAGCTACC+AGG 0.475118 1.1:+1929131 None:intergenic
TTGGTCTTCCTAAGAGGAAA+GGG 0.483400 1.1:-1929342 MS.gene24266:CDS
GCTGGTGAAGTTGGGATTCG+AGG 0.483854 1.1:-1929556 MS.gene24266:CDS
TGCTGCTTGTTATTGTTGAA+AGG 0.487258 1.1:-1929792 MS.gene24266:CDS
AGGAAGAAGGAGAAAATGAA+AGG 0.488535 1.1:-1929619 MS.gene24266:CDS
AACAAGCAGCATTGCATCTA+CGG 0.491489 1.1:+1929804 None:intergenic
ATTGGATCAATTTGCTTGAA+AGG 0.495330 1.1:-1929087 MS.gene24266:CDS
TACAGCCTACTAGGGTGGAT+TGG 0.497105 1.1:-1929105 MS.gene24266:CDS
GTTATCGGCTTCGCCTCCTT+CGG 0.498408 1.1:+1929226 None:intergenic
GTAGATTTGCTGGTGAAGTT+GGG 0.499570 1.1:-1929564 MS.gene24266:CDS
GCGATTTGCTTAGAGAGCTT+AGG 0.500284 1.1:-1929534 MS.gene24266:CDS
TGTTGACCCTGGTAGCTGAA+TGG 0.506521 1.1:-1929138 MS.gene24266:CDS
GCTGCTTGTTATTGTTGAAA+GGG 0.513685 1.1:-1929791 MS.gene24266:CDS
GGAAGAAGGAGAAAATGAAA+GGG 0.516139 1.1:-1929618 MS.gene24266:CDS
TATGAATAAAGAGAGGAAGA+AGG 0.517752 1.1:-1929632 MS.gene24266:CDS
GGAAAAGGGTAGTGGTAGTG+GGG 0.518104 1.1:-1929755 MS.gene24266:CDS
AGGTTGTTGGTCTTCCTAAG+AGG 0.522381 1.1:-1929348 MS.gene24266:CDS
GAAAAGGGTAGTGGTAGTGG+GGG 0.524024 1.1:-1929754 MS.gene24266:CDS
GAAAATGTCGTTGTTGATGC+TGG 0.527609 1.1:-1929403 MS.gene24266:CDS
TGGTAATGTTGAGGAGAAGA+AGG 0.529708 1.1:-1929368 MS.gene24266:CDS
TTAGAAATGGAAAAGGGTAG+TGG 0.542276 1.1:-1929763 MS.gene24266:CDS
AATTCTGTCGTTGAAGGAGA+AGG 0.546483 1.1:-1929176 MS.gene24266:CDS
GTTGGTCTTCCTAAGAGGAA+AGG 0.559694 1.1:-1929343 MS.gene24266:CDS
AGGAGGCGAAGCCGATAACC+GGG 0.567405 1.1:-1929222 MS.gene24266:CDS
AGAAGTTATTTGGCCGAAGG+AGG 0.576981 1.1:-1929239 MS.gene24266:CDS
GGCTGATAAGATTCATGAGG+CGG 0.580784 1.1:-1929263 MS.gene24266:CDS
GGGAGAAGTTATTTGGCCGA+AGG 0.586632 1.1:-1929242 MS.gene24266:CDS
GCTGATAAGATTCATGAGGC+GGG 0.589093 1.1:-1929262 MS.gene24266:CDS
TTTATGGAGGACTGTGTGGG+AGG 0.601402 1.1:-1929677 MS.gene24266:CDS
ATCTTCGCGATTTGATTCGG+TGG 0.603612 1.1:-1929713 MS.gene24266:CDS
AGGATTTATGGAGGACTGTG+TGG 0.605077 1.1:-1929681 MS.gene24266:CDS
CTGTCGTTGAAGGAGAAGGA+TGG 0.608593 1.1:-1929172 MS.gene24266:CDS
GGATCCTAAATGGGAACAAG+TGG 0.618929 1.1:-1929284 MS.gene24266:CDS
CTCTACCCATTCAGCTACCA+GGG 0.620249 1.1:+1929132 None:intergenic
GCTTCTACAGCCTACTAGGG+TGG 0.622270 1.1:-1929110 MS.gene24266:CDS
TCGTGTTGATGGTAATGTTG+AGG 0.627189 1.1:-1929377 MS.gene24266:CDS
AGAGCTTCTACAGCCTACTA+GGG 0.634855 1.1:-1929113 MS.gene24266:CDS
GGATTTATGGAGGACTGTGT+GGG 0.636168 1.1:-1929680 MS.gene24266:CDS
TGGTTGATATGAATAAAGAG+AGG 0.637788 1.1:-1929639 MS.gene24266:CDS
AGATGATAGTTTGTTGACCC+TGG 0.658807 1.1:-1929149 MS.gene24266:CDS
AGTGGCTGATAAGATTCATG+AGG 0.693084 1.1:-1929266 MS.gene24266:CDS
ATGGGCACGTGAGAAAATGG+AGG 0.695770 1.1:-1929506 MS.gene24266:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAGTTCAAGATTTA+TGG - chr1.1:1929561-1929580 MS.gene24266:CDS 15.0%
!! AAATTAAGTTCAAGATTTAT+GGG - chr1.1:1929562-1929581 MS.gene24266:CDS 15.0%
! GAATTATCCTTTATACTTCA+AGG - chr1.1:1929827-1929846 MS.gene24266:CDS 25.0%
!!! GTTGTTTTAGAAATGGAAAA+GGG - chr1.1:1929111-1929130 MS.gene24266:CDS 25.0%
ACAGTTACCTTGAAGTATAA+AGG + chr1.1:1929837-1929856 None:intergenic 30.0%
TAGAGAGCTTAGGTTTAAAT+GGG - chr1.1:1929356-1929375 MS.gene24266:CDS 30.0%
TATGAATAAAGAGAGGAAGA+AGG - chr1.1:1929248-1929267 MS.gene24266:CDS 30.0%
TTAGAGAGCTTAGGTTTAAA+TGG - chr1.1:1929355-1929374 MS.gene24266:CDS 30.0%
! ATCAACAACGACATTTTCTT+CGG + chr1.1:1929475-1929494 None:intergenic 30.0%
! ATTGGATCAATTTGCTTGAA+AGG - chr1.1:1929793-1929812 MS.gene24266:CDS 30.0%
! TACGGTACTTTTATCACATT+CGG + chr1.1:1929061-1929080 None:intergenic 30.0%
! TGGTTGATATGAATAAAGAG+AGG - chr1.1:1929241-1929260 MS.gene24266:CDS 30.0%
! TTAATTTTCCCTTTCCTCTT+AGG + chr1.1:1929549-1929568 None:intergenic 30.0%
!! ACATGAGTTTTATGAGGATT+TGG - chr1.1:1929398-1929417 MS.gene24266:CDS 30.0%
!! ATTTTAGTGAGGATTTATGG+AGG - chr1.1:1929190-1929209 MS.gene24266:CDS 30.0%
!! TGGATTTTAGTGAGGATTTA+TGG - chr1.1:1929187-1929206 MS.gene24266:CDS 30.0%
!!! GGTTGTTTTAGAAATGGAAA+AGG - chr1.1:1929110-1929129 MS.gene24266:CDS 30.0%
!!! TGAAAGGGTTGTTTTAGAAA+TGG - chr1.1:1929104-1929123 MS.gene24266:CDS 30.0%
AAAGAGATGTGTAGATTTGC+TGG - chr1.1:1929306-1929325 MS.gene24266:CDS 35.0%
AGGAAGAAGGAGAAAATGAA+AGG - chr1.1:1929261-1929280 MS.gene24266:CDS 35.0%
ATGAGGATTTGGAGAAAATG+AGG - chr1.1:1929409-1929428 MS.gene24266:CDS 35.0%
GATTTATGGGCTTGATCTAT+CGG - chr1.1:1929575-1929594 MS.gene24266:CDS 35.0%
GGAAGAAGGAGAAAATGAAA+GGG - chr1.1:1929262-1929281 MS.gene24266:CDS 35.0%
TGATCTATCGGATCCTAAAT+GGG - chr1.1:1929587-1929606 MS.gene24266:CDS 35.0%
TTAGAAATGGAAAAGGGTAG+TGG - chr1.1:1929117-1929136 MS.gene24266:CDS 35.0%
TTGATCTATCGGATCCTAAA+TGG - chr1.1:1929586-1929605 MS.gene24266:CDS 35.0%
! ACGGTACTTTTATCACATTC+GGG + chr1.1:1929060-1929079 None:intergenic 35.0%
! CTTCAACGACAGAATTTTCT+CGG + chr1.1:1929700-1929719 None:intergenic 35.0%
! GGAGGTTAGTAATAAGGTTT+TGG - chr1.1:1929221-1929240 MS.gene24266:CDS 35.0%
!! GCTGCTTGTTATTGTTGAAA+GGG - chr1.1:1929089-1929108 MS.gene24266:CDS 35.0%
!! TGCTGCTTGTTATTGTTGAA+AGG - chr1.1:1929088-1929107 MS.gene24266:CDS 35.0%
AACAAGCAGCATTGCATCTA+CGG + chr1.1:1929079-1929098 None:intergenic 40.0%
AATTCTGTCGTTGAAGGAGA+AGG - chr1.1:1929704-1929723 MS.gene24266:CDS 40.0%
AGTGGCTGATAAGATTCATG+AGG - chr1.1:1929614-1929633 MS.gene24266:CDS 40.0%
CGAGAAAATTCTGTCGTTGA+AGG - chr1.1:1929698-1929717 MS.gene24266:CDS 40.0%
GAAAATGTCGTTGTTGATGC+TGG - chr1.1:1929477-1929496 MS.gene24266:CDS 40.0%
TAAATGGGCACGTGAGAAAA+TGG - chr1.1:1929371-1929390 MS.gene24266:CDS 40.0%
TGCATCTTCGCGATTTGATT+CGG - chr1.1:1929164-1929183 MS.gene24266:CDS 40.0%
TGTGTGGGAGGTTAGTAATA+AGG - chr1.1:1929215-1929234 MS.gene24266:CDS 40.0%
TGTTTGCATTTCCCGGTTAT+CGG + chr1.1:1929672-1929691 None:intergenic 40.0%
! CGGTACTTTTATCACATTCG+GGG + chr1.1:1929059-1929078 None:intergenic 40.0%
! TGGTAATGTTGAGGAGAAGA+AGG - chr1.1:1929512-1929531 MS.gene24266:CDS 40.0%
! TTGGTCTTCCTAAGAGGAAA+GGG - chr1.1:1929538-1929557 MS.gene24266:CDS 40.0%
!! AGATGATAGTTTGTTGACCC+TGG - chr1.1:1929731-1929750 MS.gene24266:CDS 40.0%
!! GGAGGAACATGAGTTTTATG+AGG - chr1.1:1929392-1929411 MS.gene24266:CDS 40.0%
!! GGTAACTTGTTTGCATTTCC+CGG + chr1.1:1929679-1929698 None:intergenic 40.0%
!! GTAGATTTGCTGGTGAAGTT+GGG - chr1.1:1929316-1929335 MS.gene24266:CDS 40.0%
!! TCGTGTTGATGGTAATGTTG+AGG - chr1.1:1929503-1929522 MS.gene24266:CDS 40.0%
!! TGTAGATTTGCTGGTGAAGT+TGG - chr1.1:1929315-1929334 MS.gene24266:CDS 40.0%
!!! TGATTCGGTGGATTTTAGTG+AGG - chr1.1:1929179-1929198 MS.gene24266:CDS 40.0%
AGAAGTTATTTGGCCGAAGG+AGG - chr1.1:1929641-1929660 MS.gene24266:CDS 45.0%
AGAGCTTCTACAGCCTACTA+GGG - chr1.1:1929767-1929786 MS.gene24266:CDS 45.0%
AGGATTTATGGAGGACTGTG+TGG - chr1.1:1929199-1929218 MS.gene24266:CDS 45.0%
ATGAGGCGGGAGAAGTTATT+TGG - chr1.1:1929631-1929650 MS.gene24266:CDS 45.0%
GCTGATAAGATTCATGAGGC+GGG - chr1.1:1929618-1929637 MS.gene24266:CDS 45.0%
GGATCCTAAATGGGAACAAG+TGG - chr1.1:1929596-1929615 MS.gene24266:CDS 45.0%
GGATTTATGGAGGACTGTGT+GGG - chr1.1:1929200-1929219 MS.gene24266:CDS 45.0%
GGCTGATAAGATTCATGAGG+CGG - chr1.1:1929617-1929636 MS.gene24266:CDS 45.0%
GTTGAGGAGAAGAAGGTTGT+TGG - chr1.1:1929519-1929538 MS.gene24266:CDS 45.0%
TAGAGCTTCTACAGCCTACT+AGG - chr1.1:1929766-1929785 MS.gene24266:CDS 45.0%
TCAGCCACTTGTTCCCATTT+AGG + chr1.1:1929603-1929622 None:intergenic 45.0%
TTGATCCAATCCACCCTAGT+AGG + chr1.1:1929783-1929802 None:intergenic 45.0%
! ATGGAAAAGGGTAGTGGTAG+TGG - chr1.1:1929123-1929142 MS.gene24266:CDS 45.0%
! GCGATTTGCTTAGAGAGCTT+AGG - chr1.1:1929346-1929365 MS.gene24266:CDS 45.0%
! TGGAAAAGGGTAGTGGTAGT+GGG - chr1.1:1929124-1929143 MS.gene24266:CDS 45.0%
!! AGGTTGTTGGTCTTCCTAAG+AGG - chr1.1:1929532-1929551 MS.gene24266:CDS 45.0%
!! ATCTTCGCGATTTGATTCGG+TGG - chr1.1:1929167-1929186 MS.gene24266:CDS 45.0%
!! GTTGGTCTTCCTAAGAGGAA+AGG - chr1.1:1929537-1929556 MS.gene24266:CDS 45.0%
ATGGGCACGTGAGAAAATGG+AGG - chr1.1:1929374-1929393 MS.gene24266:CDS 50.0%
CTCTACCCATTCAGCTACCA+GGG + chr1.1:1929751-1929770 None:intergenic 50.0%
CTGTCGTTGAAGGAGAAGGA+TGG - chr1.1:1929708-1929727 MS.gene24266:CDS 50.0%
GATGCTGGTGTTCGTGTTGA+TGG - chr1.1:1929492-1929511 MS.gene24266:CDS 50.0%
GGGAGAAGTTATTTGGCCGA+AGG - chr1.1:1929638-1929657 MS.gene24266:CDS 50.0%
GTTGACCCTGGTAGCTGAAT+GGG - chr1.1:1929743-1929762 MS.gene24266:CDS 50.0%
TACAGCCTACTAGGGTGGAT+TGG - chr1.1:1929775-1929794 MS.gene24266:CDS 50.0%
TGTTGACCCTGGTAGCTGAA+TGG - chr1.1:1929742-1929761 MS.gene24266:CDS 50.0%
TTTATGGAGGACTGTGTGGG+AGG - chr1.1:1929203-1929222 MS.gene24266:CDS 50.0%
! GGAAAAGGGTAGTGGTAGTG+GGG - chr1.1:1929125-1929144 MS.gene24266:CDS 50.0%
! TAGTGGGGGTGGTGATGAAA+AGG - chr1.1:1929140-1929159 MS.gene24266:CDS 50.0%
!! GAAAAGGGTAGTGGTAGTGG+GGG - chr1.1:1929126-1929145 MS.gene24266:CDS 50.0%
AAGGAGGCGAAGCCGATAAC+CGG - chr1.1:1929657-1929676 MS.gene24266:CDS 55.0%
GCTCTACCCATTCAGCTACC+AGG + chr1.1:1929752-1929771 None:intergenic 55.0%
GCTGGTGAAGTTGGGATTCG+AGG - chr1.1:1929324-1929343 MS.gene24266:CDS 55.0%
GCTTCTACAGCCTACTAGGG+TGG - chr1.1:1929770-1929789 MS.gene24266:CDS 55.0%
!! GTTATCGGCTTCGCCTCCTT+CGG + chr1.1:1929657-1929676 None:intergenic 55.0%
AGGAGGCGAAGCCGATAACC+GGG - chr1.1:1929658-1929677 MS.gene24266:CDS 60.0%
!! AAGGGTAGTGGTAGTGGGGG+TGG - chr1.1:1929129-1929148 MS.gene24266:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 1929030 1929872 1929030 ID=MS.gene24266
chr1.1 mRNA 1929030 1929872 1929030 ID=MS.gene24266.t1;Parent=MS.gene24266
chr1.1 exon 1929030 1929872 1929030 ID=MS.gene24266.t1.exon1;Parent=MS.gene24266.t1
chr1.1 CDS 1929030 1929872 1929030 ID=cds.MS.gene24266.t1;Parent=MS.gene24266.t1
Gene Sequence

>MS.gene24266

ATGCGAAAAAAAGTTAAAGAAGTTTACCCCGAATGTGATAAAAGTACCGTAGATGCAATGCTGCTTGTTATTGTTGAAAGGGTTGTTTTAGAAATGGAAAAGGGTAGTGGTAGTGGGGGTGGTGATGAAAAGGTTGCATCTTCGCGATTTGATTCGGTGGATTTTAGTGAGGATTTATGGAGGACTGTGTGGGAGGTTAGTAATAAGGTTTTGGTTGATATGAATAAAGAGAGGAAGAAGGAGAAAATGAAAGGGTTTTTGCAGTGTGATGAAGTGAAAGAGATGTGTAGATTTGCTGGTGAAGTTGGGATTCGAGGCGATTTGCTTAGAGAGCTTAGGTTTAAATGGGCACGTGAGAAAATGGAGGAACATGAGTTTTATGAGGATTTGGAGAAAATGAGGAAAGAGACTCAGATTGTTGATGAAGAAATAAAAAATGAAACCGAAGAAAATGTCGTTGTTGATGCTGGTGTTCGTGTTGATGGTAATGTTGAGGAGAAGAAGGTTGTTGGTCTTCCTAAGAGGAAAGGGAAAATTAAGTTCAAGATTTATGGGCTTGATCTATCGGATCCTAAATGGGAACAAGTGGCTGATAAGATTCATGAGGCGGGAGAAGTTATTTGGCCGAAGGAGGCGAAGCCGATAACCGGGAAATGCAAACAAGTTACCGAGAAAATTCTGTCGTTGAAGGAGAAGGATGGAGATGATAGTTTGTTGACCCTGGTAGCTGAATGGGTAGAGCTTCTACAGCCTACTAGGGTGGATTGGATCAATTTGCTTGAAAGGTTGAAAAATCAGAATTATCCTTTATACTTCAAGGTAACTGTTGCATTGCCTTTTTAG

Protein sequence

>MS.gene24266.t1

MRKKVKEVYPECDKSTVDAMLLVIVERVVLEMEKGSGSGGGDEKVASSRFDSVDFSEDLWRTVWEVSNKVLVDMNKERKKEKMKGFLQCDEVKEMCRFAGEVGIRGDLLRELRFKWAREKMEEHEFYEDLEKMRKETQIVDEEIKNETEENVVVDAGVRVDGNVEEKKVVGLPKRKGKIKFKIYGLDLSDPKWEQVADKIHEAGEVIWPKEAKPITGKCKQVTEKILSLKEKDGDDSLLTLVAEWVELLQPTRVDWINLLERLKNQNYPLYFKVTVALPF