AlfalfaGEDB | MS.gene24736

M. sativa cultivar XinJiangDaYe / MS.gene24736

KEGG: K17479
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009055 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24736.t1	AFK41682.1	96.6	87	3	0	1	87	301	387	9.80E-41	175.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24736.t1	Q9LH89	41.8	91	41	2	1	79	291	381	3.7e-12	72.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24736.t1	G7KQV9	96.6	87	3	0	1	87	301	387	7.0e-41	175.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene24736	0.807263	5.50E-50	-1.69E-46
MS.gene049350	MS.gene24736	0.821835	3.27E-53	-1.69E-46
MS.gene049501	MS.gene24736	0.807165	5.78E-50	-1.69E-46
MS.gene049711	MS.gene24736	0.821243	4.48E-53	-1.69E-46
MS.gene049821	MS.gene24736	0.842585	2.36E-58	-1.69E-46
MS.gene049850	MS.gene24736	0.857889	1.20E-62	-1.69E-46
MS.gene050020	MS.gene24736	0.829269	5.66E-55	-1.69E-46
MS.gene050807	MS.gene24736	0.837701	4.45E-57	-1.69E-46
MS.gene050808	MS.gene24736	0.805653	1.20E-49	-1.69E-46
MS.gene050901	MS.gene24736	0.85254	4.32E-61	-1.69E-46
MS.gene051018	MS.gene24736	0.814943	1.19E-51	-1.69E-46
MS.gene051199	MS.gene24736	0.809138	2.19E-50	-1.69E-46
MS.gene051247	MS.gene24736	0.830986	2.16E-55	-1.69E-46
MS.gene05157	MS.gene24736	0.843508	1.34E-58	-1.69E-46
MS.gene051664	MS.gene24736	0.81991	9.06E-53	-1.69E-46
MS.gene051700	MS.gene24736	0.81033	1.22E-50	-1.69E-46
MS.gene051719	MS.gene24736	0.88945	2.40E-73	-1.69E-46
MS.gene051720	MS.gene24736	0.891278	4.60E-74	-1.69E-46
MS.gene051744	MS.gene24736	0.814207	1.73E-51	-1.69E-46
MS.gene052000	MS.gene24736	0.807457	5.01E-50	-1.69E-46
MS.gene052020	MS.gene24736	0.807996	3.85E-50	-1.69E-46
MS.gene052133	MS.gene24736	0.83138	1.73E-55	-1.69E-46
MS.gene052516	MS.gene24736	0.82894	6.80E-55	-1.69E-46
MS.gene052582	MS.gene24736	0.807172	5.75E-50	-1.69E-46
MS.gene052584	MS.gene24736	0.807368	5.23E-50	-1.69E-46
MS.gene052665	MS.gene24736	0.810071	1.38E-50	-1.69E-46
MS.gene052673	MS.gene24736	-0.809887	1.52E-50	-1.69E-46
MS.gene052732	MS.gene24736	0.816265	6.05E-52	-1.69E-46
MS.gene052896	MS.gene24736	0.805335	1.40E-49	-1.69E-46
MS.gene053122	MS.gene24736	0.803309	3.71E-49	-1.69E-46
MS.gene053144	MS.gene24736	0.851151	1.07E-60	-1.69E-46
MS.gene053164	MS.gene24736	0.816457	5.48E-52	-1.69E-46
MS.gene053174	MS.gene24736	0.807007	6.24E-50	-1.69E-46
MS.gene053226	MS.gene24736	0.828387	9.25E-55	-1.69E-46
MS.gene053581	MS.gene24736	0.863628	2.17E-64	-1.69E-46
MS.gene053599	MS.gene24736	0.818726	1.69E-52	-1.69E-46
MS.gene05364	MS.gene24736	0.81512	1.09E-51	-1.69E-46
MS.gene05423	MS.gene24736	0.828199	1.03E-54	-1.69E-46
MS.gene054584	MS.gene24736	0.854244	1.40E-61	-1.69E-46
MS.gene054651	MS.gene24736	0.822911	1.84E-53	-1.69E-46
MS.gene054775	MS.gene24736	0.809416	1.91E-50	-1.69E-46
MS.gene054791	MS.gene24736	0.815344	9.70E-52	-1.69E-46
MS.gene054991	MS.gene24736	0.810378	1.19E-50	-1.69E-46
MS.gene055144	MS.gene24736	0.81413	1.80E-51	-1.69E-46
MS.gene055465	MS.gene24736	0.872526	2.97E-67	-1.69E-46
MS.gene055466	MS.gene24736	0.876258	1.61E-68	-1.69E-46
MS.gene055468	MS.gene24736	0.870433	1.46E-66	-1.69E-46
MS.gene055469	MS.gene24736	0.840018	1.12E-57	-1.69E-46
MS.gene055513	MS.gene24736	0.805542	1.27E-49	-1.69E-46
MS.gene055537	MS.gene24736	0.856485	3.12E-62	-1.69E-46
MS.gene055580	MS.gene24736	-0.845929	2.99E-59	-1.69E-46
MS.gene055890	MS.gene24736	0.813543	2.43E-51	-1.69E-46
MS.gene055891	MS.gene24736	0.817131	3.87E-52	-1.69E-46
MS.gene056005	MS.gene24736	0.802424	5.65E-49	-1.69E-46
MS.gene056043	MS.gene24736	-0.821368	4.19E-53	-1.69E-46
MS.gene056215	MS.gene24736	0.808856	2.52E-50	-1.69E-46
MS.gene056308	MS.gene24736	0.848071	7.74E-60	-1.69E-46
MS.gene056366	MS.gene24736	0.802626	5.13E-49	-1.69E-46
MS.gene056507	MS.gene24736	0.809951	1.47E-50	-1.69E-46
MS.gene056553	MS.gene24736	0.800061	1.72E-48	-1.69E-46
MS.gene056595	MS.gene24736	0.809137	2.20E-50	-1.69E-46
MS.gene057446	MS.gene24736	0.803634	3.17E-49	-1.69E-46
MS.gene057915	MS.gene24736	0.825891	3.66E-54	-1.69E-46
MS.gene058087	MS.gene24736	0.823499	1.34E-53	-1.69E-46
MS.gene058339	MS.gene24736	0.816353	5.78E-52	-1.69E-46
MS.gene058625	MS.gene24736	0.840945	6.40E-58	-1.69E-46
MS.gene05894	MS.gene24736	0.80716	5.79E-50	-1.69E-46
MS.gene059015	MS.gene24736	0.809997	1.43E-50	-1.69E-46
MS.gene059084	MS.gene24736	0.821829	3.28E-53	-1.69E-46
MS.gene059625	MS.gene24736	0.817656	2.94E-52	-1.69E-46
MS.gene059725	MS.gene24736	0.802973	4.35E-49	-1.69E-46
MS.gene060519	MS.gene24736	0.859549	3.83E-63	-1.69E-46
MS.gene060587	MS.gene24736	0.857112	2.04E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24736.t1	MTR_7g104720	96.552	87	3	0	1	87	301	387	3.29e-54	173
MS.gene24736.t1	MTR_5g083110	45.882	85	45	1	1	84	135	219	1.82e-20	82.0
MS.gene24736.t1	MTR_1g090400	49.425	87	41	2	1	85	265	350	2.36e-20	83.2
MS.gene24736.t1	MTR_3g465550	49.412	85	41	2	1	83	128	212	6.09e-19	77.8
MS.gene24736.t1	MTR_3g071510	41.176	85	49	1	1	84	131	215	7.08e-19	77.8
MS.gene24736.t1	MTR_3g017490	43.023	86	47	1	1	84	155	240	1.73e-18	77.4
MS.gene24736.t1	MTR_7g090230	41.573	89	49	2	1	86	318	406	1.32e-17	75.9
MS.gene24736.t1	MTR_1g051180	43.182	88	48	2	1	86	249	336	4.06e-17	74.3
MS.gene24736.t1	MTR_8g106900	50.562	89	40	1	1	85	163	251	4.28e-17	73.6
MS.gene24736.t1	MTR_5g072400	40.476	84	49	1	1	83	150	233	1.63e-16	71.6
MS.gene24736.t1	MTR_8g038440	44.944	89	44	2	1	84	146	234	1.74e-16	71.6
MS.gene24736.t1	MTR_7g106500	40.244	82	46	2	1	80	166	246	8.71e-15	67.4
MS.gene24736.t1	MTR_8g099665	49.254	67	30	1	18	80	277	343	1.01e-13	64.7
MS.gene24736.t1	MTR_7g079015	39.560	91	46	2	1	82	1	91	1.04e-13	61.2
MS.gene24736.t1	MTR_6g011640	39.560	91	46	2	1	82	273	363	1.22e-13	64.7
MS.gene24736.t1	MTR_7g010090	47.619	84	39	2	1	79	307	390	4.72e-13	63.2
MS.gene24736.t1	MTR_1g095960	40.230	87	43	2	5	82	32	118	7.11e-13	59.7
MS.gene24736.t1	MTR_2g026830	36.782	87	50	2	1	82	1	87	5.18e-12	57.0
MS.gene24736.t1	MTR_2g028040	38.636	88	44	2	5	82	5	92	1.92e-11	58.5
MS.gene24736.t1	MTR_3g030520	36.364	88	47	2	1	79	175	262	6.77e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24736.t1	AT5G03870	62.500	88	30	1	1	85	277	364	6.59e-25	95.9
MS.gene24736.t1	AT5G06470	44.048	84	46	1	1	84	129	211	3.41e-19	78.6
MS.gene24736.t1	AT5G58530	43.011	93	43	2	1	83	153	245	1.50e-18	77.4
MS.gene24736.t1	AT3G57070	41.111	90	49	2	1	86	308	397	1.52e-18	78.6
MS.gene24736.t1	AT4G10630	45.652	92	38	2	1	80	216	307	9.97e-18	75.9
MS.gene24736.t1	AT2G41330	39.080	87	50	2	1	84	294	380	1.37e-15	70.1
MS.gene24736.t1	AT3G11773	41.176	85	48	2	1	84	47	130	1.70e-15	67.0
MS.gene24736.t1	AT3G11773	41.176	85	48	2	1	84	97	180	2.58e-15	67.8
MS.gene24736.t1	AT5G13810	40.909	88	50	2	1	86	167	254	9.59e-15	67.0
MS.gene24736.t1	AT1G32760	51.136	88	36	2	1	81	201	288	1.46e-14	67.0
MS.gene24736.t1	AT3G28850	41.758	91	41	2	1	79	291	381	8.24e-14	65.1
MS.gene24736.t1	AT5G39865	39.362	94	45	2	1	82	265	358	1.38e-13	64.7
MS.gene24736.t1	AT5G01420	40.244	82	47	2	1	80	296	377	6.14e-13	62.8
MS.gene24736.t1	AT1G64500	43.678	87	40	3	1	79	252	337	1.09e-12	62.0

Find 30 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTATGGATTCCGGGTTTA+AGG	0.176980	7.1:-6862488	None:intergenic
AGTGGATGAAGTTATGAAAT+TGG	0.256695	7.1:-6862389	MS.gene24736:CDS
GGAGGAATTAAGGAAGCTTA+TGG	0.277561	7.1:-6862467	MS.gene24736:CDS
TAATGGAAGTTGTAAGGTTT+TGG	0.299182	7.1:-6862266	MS.gene24736:CDS
AGATAAATTAGGTGTTCTTT+TGG	0.307593	7.1:-6862359	MS.gene24736:CDS
CCGGGTTTAAGGAGGAATTA+AGG	0.318478	7.1:-6862477	MS.gene24736:CDS
TTGGAAGATGAAGATAAATT+AGG	0.331173	7.1:-6862370	MS.gene24736:CDS
CGTGATGTGTCTATGGATTC+CGG	0.334847	7.1:-6862496	None:intergenic
GAAGGGATTCCAAGGGCATT+AGG	0.338815	7.1:-6862337	MS.gene24736:CDS
GTGATGTGTCTATGGATTCC+GGG	0.405890	7.1:-6862495	None:intergenic
GTGAAGGGTGTGGAGGTTTG+AGG	0.418799	7.1:-6862309	MS.gene24736:CDS
GAGGAATTAAGGAAGCTTAT+GGG	0.421784	7.1:-6862466	MS.gene24736:CDS
GGAATGTAATGGAAGTTGTA+AGG	0.427272	7.1:-6862272	MS.gene24736:CDS
TTTGAGGTTTGTGATGTGTA+AGG	0.440858	7.1:-6862293	MS.gene24736:CDS
TTTGTGAAGGGAAGGTTTGT+TGG	0.445919	7.1:-6862415	MS.gene24736:CDS
GGATGAGAAGCAAAAGAAAA+CGG	0.491051	7.1:-6862245	None:intergenic
CTGTTGTGTTTGTGAAGGGA+AGG	0.492747	7.1:-6862423	MS.gene24736:CDS
GTGAAGGGAAGGTTTGTTGG+AGG	0.493959	7.1:-6862412	MS.gene24736:CDS
CTTCCCTTCACAAACACAAC+AGG	0.496211	7.1:+6862424	None:intergenic
TTAGGAGTTTGTGAAGGGTG+TGG	0.502170	7.1:-6862319	MS.gene24736:CDS
GGGAAGGTTTGTTGGAGGAG+TGG	0.516015	7.1:-6862407	MS.gene24736:CDS
TCACAAACTCCTAATGCCCT+TGG	0.517790	7.1:+6862328	None:intergenic
CCTTAATTCCTCCTTAAACC+CGG	0.526472	7.1:+6862477	None:intergenic
GGAGTTTGTGAAGGGTGTGG+AGG	0.564654	7.1:-6862316	MS.gene24736:CDS
AGGGCATTAGGAGTTTGTGA+AGG	0.584030	7.1:-6862325	MS.gene24736:CDS
AGTTCCTGTTGTGTTTGTGA+AGG	0.590978	7.1:-6862428	MS.gene24736:CDS
GTTCCTGTTGTGTTTGTGAA+GGG	0.598376	7.1:-6862427	MS.gene24736:CDS
GGGCATTAGGAGTTTGTGAA+GGG	0.600002	7.1:-6862324	MS.gene24736:CDS
TATGGATTCCGGGTTTAAGG+AGG	0.617441	7.1:-6862485	MS.gene24736:CDS
GTGATGTGTAAGGAATGTAA+TGG	0.630771	7.1:-6862283	MS.gene24736:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGGAAGATGAAGATAAATT+AGG	-	chr7.1:6862360-6862379	MS.gene24736:CDS	25.0%
!!	AGATAAATTAGGTGTTCTTT+TGG	-	chr7.1:6862371-6862390	MS.gene24736:CDS	25.0%
	AGTGGATGAAGTTATGAAAT+TGG	-	chr7.1:6862341-6862360	MS.gene24736:CDS	30.0%
!	AAATTAGGTGTTCTTTTGGA+AGG	-	chr7.1:6862375-6862394	MS.gene24736:CDS	30.0%
!	TAATGGAAGTTGTAAGGTTT+TGG	-	chr7.1:6862464-6862483	MS.gene24736:CDS	30.0%
!!	AATTAGGTGTTCTTTTGGAA+GGG	-	chr7.1:6862376-6862395	MS.gene24736:CDS	30.0%
	GAGGAATTAAGGAAGCTTAT+GGG	-	chr7.1:6862264-6862283	MS.gene24736:CDS	35.0%
	GTGATGTGTAAGGAATGTAA+TGG	-	chr7.1:6862447-6862466	MS.gene24736:CDS	35.0%
!	GGAATGTAATGGAAGTTGTA+AGG	-	chr7.1:6862458-6862477	MS.gene24736:CDS	35.0%
!	TTTGAGGTTTGTGATGTGTA+AGG	-	chr7.1:6862437-6862456	MS.gene24736:CDS	35.0%
	AGTTCCTGTTGTGTTTGTGA+AGG	-	chr7.1:6862302-6862321	MS.gene24736:CDS	40.0%
	CCTTAATTCCTCCTTAAACC+CGG	+	chr7.1:6862256-6862275	None:intergenic	40.0%
	GGAGGAATTAAGGAAGCTTA+TGG	-	chr7.1:6862263-6862282	MS.gene24736:CDS	40.0%
	GTTCCTGTTGTGTTTGTGAA+GGG	-	chr7.1:6862303-6862322	MS.gene24736:CDS	40.0%
	TTTGTGAAGGGAAGGTTTGT+TGG	-	chr7.1:6862315-6862334	MS.gene24736:CDS	40.0%
!!	TCTTTTGGAAGGGATTCCAA+GGG	-	chr7.1:6862386-6862405	MS.gene24736:CDS	40.0%
!!	TTCTTTTGGAAGGGATTCCA+AGG	-	chr7.1:6862385-6862404	MS.gene24736:CDS	40.0%
	CCGGGTTTAAGGAGGAATTA+AGG	-	chr7.1:6862253-6862272	MS.gene24736:CDS	45.0%
	CTGTTGTGTTTGTGAAGGGA+AGG	-	chr7.1:6862307-6862326	MS.gene24736:CDS	45.0%
	CTTCCCTTCACAAACACAAC+AGG	+	chr7.1:6862309-6862328	None:intergenic	45.0%
	GGGCATTAGGAGTTTGTGAA+GGG	-	chr7.1:6862406-6862425	MS.gene24736:CDS	45.0%
	TCACAAACTCCTAATGCCCT+TGG	+	chr7.1:6862405-6862424	None:intergenic	45.0%
	TTAGGAGTTTGTGAAGGGTG+TGG	-	chr7.1:6862411-6862430	MS.gene24736:CDS	45.0%
!	AGGGCATTAGGAGTTTGTGA+AGG	-	chr7.1:6862405-6862424	MS.gene24736:CDS	45.0%
!!	GAAGGGATTCCAAGGGCATT+AGG	-	chr7.1:6862393-6862412	MS.gene24736:CDS	50.0%
!!	GTGAAGGGAAGGTTTGTTGG+AGG	-	chr7.1:6862318-6862337	MS.gene24736:CDS	50.0%
!	GGAGTTTGTGAAGGGTGTGG+AGG	-	chr7.1:6862414-6862433	MS.gene24736:CDS	55.0%
!	GTGAAGGGTGTGGAGGTTTG+AGG	-	chr7.1:6862421-6862440	MS.gene24736:CDS	55.0%
!!	GGGAAGGTTTGTTGGAGGAG+TGG	-	chr7.1:6862323-6862342	MS.gene24736:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	6862246	6862506	6862246	ID=MS.gene24736
chr7.1	mRNA	6862246	6862506	6862246	ID=MS.gene24736.t1;Parent=MS.gene24736
chr7.1	exon	6862246	6862506	6862246	ID=MS.gene24736.t1.exon1;Parent=MS.gene24736.t1
chr7.1	CDS	6862246	6862506	6862246	ID=cds.MS.gene24736.t1;Parent=MS.gene24736.t1

Gene Sequence	>MS.gene24736 ATGGATTCCGGGTTTAAGGAGGAATTAAGGAAGCTTATGGGAATGAAACAAGTTCAAGTTCCTGTTGTGTTTGTGAAGGGAAGGTTTGTTGGAGGAGTGGATGAAGTTATGAAATTGGAAGATGAAGATAAATTAGGTGTTCTTTTGGAAGGGATTCCAAGGGCATTAGGAGTTTGTGAAGGGTGTGGAGGTTTGAGGTTTGTGATGTGTAAGGAATGTAATGGAAGTTGTAAGGTTTTGGATGAGAAGCAAAAGAAAACG
Protein sequence	>MS.gene24736.t1 MDSGFKEELRKLMGMKQVQVPVVFVKGRFVGGVDEVMKLEDEDKLGVLLEGIPRALGVCEGCGGLRFVMCKECNGSCKVLDEKQKKT