Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24907.t1 | KEH28774.1 | 97.3 | 75 | 2 | 0 | 1 | 75 | 1 | 75 | 2.70E-31 | 144.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24907.t1 | A0A072USC8 | 97.3 | 75 | 2 | 0 | 1 | 75 | 1 | 75 | 2.0e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene24907 | 0.805547 | 1.27E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene24907 | 0.822779 | 1.97E-53 | -1.69E-46 |
| MS.gene049368 | MS.gene24907 | 0.803294 | 3.73E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene24907 | 0.820827 | 5.58E-53 | -1.69E-46 |
| MS.gene050085 | MS.gene24907 | 0.813251 | 2.81E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene24907 | 0.829368 | 5.35E-55 | -1.69E-46 |
| MS.gene050361 | MS.gene24907 | 0.822128 | 2.80E-53 | -1.69E-46 |
| MS.gene050386 | MS.gene24907 | 0.800546 | 1.37E-48 | -1.69E-46 |
| MS.gene050414 | MS.gene24907 | 0.821107 | 4.81E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene24907 | 0.848889 | 4.60E-60 | -1.69E-46 |
| MS.gene050654 | MS.gene24907 | 0.842337 | 2.75E-58 | -1.69E-46 |
| MS.gene050689 | MS.gene24907 | 0.810189 | 1.30E-50 | -1.69E-46 |
| MS.gene050752 | MS.gene24907 | 0.817939 | 2.54E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene24907 | 0.811672 | 6.23E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene24907 | 0.848717 | 5.13E-60 | -1.69E-46 |
| MS.gene051233 | MS.gene24907 | 0.841828 | 3.75E-58 | -1.69E-46 |
| MS.gene051234 | MS.gene24907 | 0.826372 | 2.81E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene24907 | 0.835663 | 1.47E-56 | -1.69E-46 |
| MS.gene051856 | MS.gene24907 | 0.813389 | 2.62E-51 | -1.69E-46 |
| MS.gene051902 | MS.gene24907 | 0.833788 | 4.36E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene24907 | 0.807924 | 3.98E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene24907 | 0.842159 | 3.06E-58 | -1.69E-46 |
| MS.gene052242 | MS.gene24907 | -0.807456 | 5.01E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene24907 | 0.836589 | 8.57E-57 | -1.69E-46 |
| MS.gene052335 | MS.gene24907 | 0.800064 | 1.72E-48 | -1.69E-46 |
| MS.gene052342 | MS.gene24907 | 0.83181 | 1.35E-55 | -1.69E-46 |
| MS.gene052434 | MS.gene24907 | 0.838393 | 2.95E-57 | -1.69E-46 |
| MS.gene052463 | MS.gene24907 | 0.806877 | 6.65E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene24907 | 0.821534 | 3.84E-53 | -1.69E-46 |
| MS.gene052932 | MS.gene24907 | 0.829782 | 4.24E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene24907 | 0.855858 | 4.76E-62 | -1.69E-46 |
| MS.gene053167 | MS.gene24907 | 0.806398 | 8.39E-50 | -1.69E-46 |
| MS.gene053456 | MS.gene24907 | 0.826833 | 2.18E-54 | -1.69E-46 |
| MS.gene054004 | MS.gene24907 | 0.8177 | 2.88E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene24907 | 0.815615 | 8.44E-52 | -1.69E-46 |
| MS.gene05517 | MS.gene24907 | 0.800027 | 1.75E-48 | -1.69E-46 |
| MS.gene055311 | MS.gene24907 | 0.808881 | 2.49E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene24907 | 0.833082 | 6.54E-56 | -1.69E-46 |
| MS.gene056045 | MS.gene24907 | 0.825954 | 3.54E-54 | -1.69E-46 |
| MS.gene056097 | MS.gene24907 | 0.813234 | 2.84E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene24907 | 0.859625 | 3.63E-63 | -1.69E-46 |
| MS.gene056390 | MS.gene24907 | 0.819414 | 1.18E-52 | -1.69E-46 |
| MS.gene056578 | MS.gene24907 | 0.82063 | 6.20E-53 | -1.69E-46 |
| MS.gene056699 | MS.gene24907 | 0.810337 | 1.21E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene24907 | 0.825802 | 3.84E-54 | -1.69E-46 |
| MS.gene057034 | MS.gene24907 | 0.800261 | 1.57E-48 | -1.69E-46 |
| MS.gene05781 | MS.gene24907 | 0.821312 | 4.32E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene24907 | 0.806463 | 8.13E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene24907 | 0.825579 | 4.34E-54 | -1.69E-46 |
| MS.gene058638 | MS.gene24907 | 0.817031 | 4.07E-52 | -1.69E-46 |
| MS.gene058639 | MS.gene24907 | 0.821375 | 4.17E-53 | -1.69E-46 |
| MS.gene059007 | MS.gene24907 | 0.807822 | 4.19E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene24907 | 0.833514 | 5.11E-56 | -1.69E-46 |
| MS.gene059965 | MS.gene24907 | 0.809855 | 1.54E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene24907 | 0.814064 | 1.86E-51 | -1.69E-46 |
| MS.gene060563 | MS.gene24907 | 0.817869 | 2.64E-52 | -1.69E-46 |
| MS.gene060932 | MS.gene24907 | 0.807148 | 5.82E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene24907 | 0.806685 | 7.30E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene24907 | 0.812706 | 3.70E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene24907 | 0.827594 | 1.43E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene24907 | 0.814101 | 1.83E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24907.t1 | MTR_4g011980 | 97.333 | 75 | 2 | 0 | 1 | 75 | 1 | 75 | 4.70e-47 | 144 |
| MS.gene24907.t1 | MTR_4g011980 | 97.333 | 75 | 2 | 0 | 1 | 75 | 1 | 75 | 2.77e-46 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24907.t1 | AT1G79985 | 74.667 | 75 | 19 | 0 | 1 | 75 | 1 | 75 | 1.78e-34 | 115 |
| MS.gene24907.t1 | AT1G79985 | 74.667 | 75 | 19 | 0 | 1 | 75 | 1 | 75 | 1.78e-34 | 115 |
| MS.gene24907.t1 | AT1G79985 | 74.667 | 75 | 19 | 0 | 1 | 75 | 1 | 75 | 1.78e-34 | 115 |
Find 20 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACTACCTCTACCGTTAC+TGG | 0.315654 | 4.1:+83864361 | MS.gene24907:CDS |
| TGGGTAGGAGTCATGTTCTA+TGG | 0.369373 | 4.1:+83864190 | None:intergenic |
| TGTTCTATGGTGCAGTGGTA+TGG | 0.393958 | 4.1:+83864203 | MS.gene24907:CDS |
| TTTCTGCTCAGCTCAGCTGC+AGG | 0.434477 | 4.1:+83864403 | MS.gene24907:CDS |
| TTGTTGGCACTCGCGTTTCT+CGG | 0.438989 | 4.1:+83864305 | MS.gene24907:CDS |
| TTATTCTTAACCGTTCTTGT+TGG | 0.460095 | 4.1:+83864289 | MS.gene24907:CDS |
| TCATATAGTACATACATTGT+AGG | 0.471364 | 4.1:-83864258 | None:intergenic |
| GTTCTATGGTGCAGTGGTAT+GGG | 0.496724 | 4.1:+83864204 | MS.gene24907:CDS |
| GTGCAGTGGTATGGGACCCT+TGG | 0.525907 | 4.1:+83864212 | MS.gene24907:CDS |
| AACGCGAGTGCCAACAAGAA+CGG | 0.560798 | 4.1:-83864299 | None:intergenic |
| TGTATGTACTATATGACACT+TGG | 0.580497 | 4.1:+83864265 | MS.gene24907:CDS |
| TTCTGCTCAGCTCAGCTGCA+GGG | 0.583677 | 4.1:+83864404 | MS.gene24907:CDS |
| CTACCTCTACCGTTACTGGT+TGG | 0.588096 | 4.1:+83864365 | MS.gene24907:CDS |
| ATTTGAGCAACAATAAGCCA+AGG | 0.604789 | 4.1:-83864229 | None:intergenic |
| GCACCAACCAGTAACGGTAG+AGG | 0.611639 | 4.1:-83864368 | None:intergenic |
| ACAAACGCACCAACCAGTAA+CGG | 0.617832 | 4.1:-83864374 | None:intergenic |
| AGTCATGTTCTATGGTGCAG+TGG | 0.646387 | 4.1:+83864198 | None:intergenic |
| AGTAACGGTAGAGGTAGTAA+CGG | 0.658848 | 4.1:-83864359 | None:intergenic |
| TTTGAGCAACAATAAGCCAA+GGG | 0.678329 | 4.1:-83864228 | None:intergenic |
| CATAGTCAAAGAAATACACA+AGG | 0.683900 | 4.1:-83864333 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCATATAGTACATACATTGT+AGG | - | chr4.1:83864261-83864280 | None:intergenic | 25.0% |
| CATAGTCAAAGAAATACACA+AGG | - | chr4.1:83864336-83864355 | None:intergenic | 30.0% | |
| TGTATGTACTATATGACACT+TGG | + | chr4.1:83864265-83864284 | MS.gene24907:CDS | 30.0% | |
| TTATTCTTAACCGTTCTTGT+TGG | + | chr4.1:83864289-83864308 | MS.gene24907:CDS | 30.0% | |
| ATTTGAGCAACAATAAGCCA+AGG | - | chr4.1:83864232-83864251 | None:intergenic | 35.0% | |
| TTTGAGCAACAATAAGCCAA+GGG | - | chr4.1:83864231-83864250 | None:intergenic | 35.0% | |
| AGTAACGGTAGAGGTAGTAA+CGG | - | chr4.1:83864362-83864381 | None:intergenic | 40.0% | |
| ACAAACGCACCAACCAGTAA+CGG | - | chr4.1:83864377-83864396 | None:intergenic | 45.0% | |
| GTTACTACCTCTACCGTTAC+TGG | + | chr4.1:83864361-83864380 | MS.gene24907:CDS | 45.0% | |
| GTTCTATGGTGCAGTGGTAT+GGG | + | chr4.1:83864204-83864223 | MS.gene24907:CDS | 45.0% | |
| TGTTCTATGGTGCAGTGGTA+TGG | + | chr4.1:83864203-83864222 | MS.gene24907:CDS | 45.0% | |
| AACGCGAGTGCCAACAAGAA+CGG | - | chr4.1:83864302-83864321 | None:intergenic | 50.0% | |
| CTACCTCTACCGTTACTGGT+TGG | + | chr4.1:83864365-83864384 | MS.gene24907:CDS | 50.0% | |
| TTGTTGGCACTCGCGTTTCT+CGG | + | chr4.1:83864305-83864324 | MS.gene24907:CDS | 50.0% | |
| GCACCAACCAGTAACGGTAG+AGG | - | chr4.1:83864371-83864390 | None:intergenic | 55.0% | |
| TTCTGCTCAGCTCAGCTGCA+GGG | + | chr4.1:83864404-83864423 | MS.gene24907:CDS | 55.0% | |
| TTTCTGCTCAGCTCAGCTGC+AGG | + | chr4.1:83864403-83864422 | MS.gene24907:CDS | 55.0% | |
| GTGCAGTGGTATGGGACCCT+TGG | + | chr4.1:83864212-83864231 | MS.gene24907:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 83864202 | 83864429 | 83864202 | ID=MS.gene24907 |
| chr4.1 | mRNA | 83864202 | 83864429 | 83864202 | ID=MS.gene24907.t1;Parent=MS.gene24907 |
| chr4.1 | exon | 83864202 | 83864429 | 83864202 | ID=MS.gene24907.t1.exon1;Parent=MS.gene24907.t1 |
| chr4.1 | CDS | 83864202 | 83864429 | 83864202 | ID=cds.MS.gene24907.t1;Parent=MS.gene24907.t1 |
| Gene Sequence |
| Protein sequence |
