AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38976


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38976.t1 MTR_8g042490 95.319 235 11 0 1 235 1 235 1.58e-160 444
MS.gene38976.t1 MTR_2g073190 77.021 235 53 1 1 234 1 235 5.96e-125 369
MS.gene38976.t1 MTR_1g029380 48.095 210 109 0 18 227 9 218 5.56e-66 204
MS.gene38976.t1 MTR_3g089125 48.357 213 109 1 18 229 9 221 1.89e-65 203
MS.gene38976.t1 MTR_3g080990 44.749 219 119 2 8 224 2 220 7.95e-56 179
MS.gene38976.t1 MTR_6g007633 41.441 222 122 4 14 228 8 228 1.03e-51 167
MS.gene38976.t1 MTR_1g028460 40.376 213 124 2 9 218 1 213 1.86e-49 162
MS.gene38976.t1 MTR_6g007637 41.204 216 123 3 10 222 5 219 3.72e-49 161
MS.gene38976.t1 MTR_8g099730 40.000 215 127 2 10 222 14 228 2.20e-48 160
MS.gene38976.t1 MTR_3g090940 37.500 216 132 2 16 228 8 223 8.54e-48 158
MS.gene38976.t1 MTR_3g090950 37.500 216 132 2 16 228 8 223 5.83e-47 156
MS.gene38976.t1 MTR_6g007623 39.189 222 129 3 14 229 5 226 1.77e-46 154
MS.gene38976.t1 MTR_5g067530 38.605 215 128 3 18 230 14 226 4.00e-41 141
MS.gene38976.t1 MTR_8g096320 38.756 209 120 4 18 222 13 217 1.60e-40 139
MS.gene38976.t1 MTR_2g007890 35.455 220 136 3 16 233 14 229 1.64e-40 140
MS.gene38976.t1 MTR_6g034600 64.228 123 32 2 113 235 1 111 3.21e-40 134
MS.gene38976.t1 MTR_3g098910 37.441 211 124 3 16 222 11 217 1.33e-38 136
MS.gene38976.t1 MTR_2g436310 41.121 214 115 4 18 226 9 216 2.51e-38 134
MS.gene38976.t1 MTR_7g007490 38.073 218 125 4 18 229 14 227 2.57e-37 131
MS.gene38976.t1 MTR_5g092600 36.453 203 126 2 23 224 18 218 4.35e-36 128
MS.gene38976.t1 MTR_7g405710 38.140 215 130 2 16 229 11 223 1.16e-35 127
MS.gene38976.t1 MTR_4g106990 36.364 198 124 2 34 229 35 232 1.34e-35 126
MS.gene38976.t1 MTR_7g405730 39.706 204 120 2 18 220 13 214 1.60e-35 127
MS.gene38976.t1 MTR_6g007610 33.621 232 136 4 12 228 6 234 2.14e-35 126
MS.gene38976.t1 MTR_8g096310 33.641 217 135 5 18 229 13 225 1.17e-33 122
MS.gene38976.t1 MTR_3g098930 37.222 180 112 1 18 197 14 192 6.57e-31 113
MS.gene38976.t1 MTR_8g099730 38.994 159 95 2 66 222 6 164 3.79e-30 111
MS.gene38976.t1 MTR_8g096320 39.073 151 85 3 76 222 15 162 7.09e-26 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38976.t1 AT3G14770 66.524 233 73 3 4 234 6 235 3.37e-104 301
MS.gene38976.t1 AT1G21460 46.919 211 112 0 18 228 9 219 6.48e-65 202
MS.gene38976.t1 AT1G21460 46.032 189 102 0 11 199 2 190 1.18e-53 172
MS.gene38976.t1 AT5G62850 37.727 220 135 2 11 228 5 224 3.57e-51 166
MS.gene38976.t1 AT4G10850 40.807 223 124 4 14 229 10 231 8.72e-51 166
MS.gene38976.t1 AT5G53190 40.670 209 121 2 18 223 10 218 1.05e-48 161
MS.gene38976.t1 AT1G66770 40.845 213 121 3 14 222 10 221 4.19e-47 157
MS.gene38976.t1 AT5G13170 38.756 209 124 2 18 224 15 221 4.17e-45 152
MS.gene38976.t1 AT5G40260 36.406 217 133 4 18 229 12 228 2.40e-42 144
MS.gene38976.t1 AT4G15920 40.385 208 118 3 18 222 9 213 4.24e-42 143
MS.gene38976.t1 AT5G23660 40.000 205 116 3 18 219 15 215 5.11e-42 144
MS.gene38976.t1 AT3G28007 39.640 222 126 5 3 221 1 217 9.49e-42 142
MS.gene38976.t1 AT3G48740 39.024 205 118 3 18 219 15 215 1.95e-41 143
MS.gene38976.t1 AT5G50800 37.838 222 129 5 18 233 13 231 1.12e-40 141
MS.gene38976.t1 AT4G25010 39.910 223 124 6 18 233 13 232 1.21e-40 140
MS.gene38976.t1 AT5G62850 36.316 190 120 1 11 199 5 194 1.27e-39 136
MS.gene38976.t1 AT2G39060 36.842 209 129 2 18 225 13 219 3.53e-39 136
MS.gene38976.t1 AT3G16690 37.019 208 125 4 18 222 9 213 1.37e-37 131
MS.gene38976.t1 AT3G16690 36.406 217 126 5 18 231 44 251 1.77e-37 132
MS.gene38976.t1 AT5G50790 38.318 214 127 3 18 229 13 223 5.58e-37 131
MS.gene38976.t1 AT5G40260 36.145 166 103 2 18 180 12 177 1.28e-31 115
MS.gene38976.t1 AT3G16690 32.624 141 89 4 18 155 9 146 5.52e-16 73.9

Find 41 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATCTCCTTCTTTCTTTA+TGG 0.090234 8.4:+49128402 MS.gene38976:CDS
GCTTACTCCATTTGTGAAAT+TGG 0.125093 8.4:+49127482 MS.gene38976:CDS
TGAATTGTAGACCCACATTT+AGG 0.291772 8.4:+49127748 MS.gene38976:intron
GTAGGTAATATCTTTGCATT+TGG 0.325780 8.4:+49127623 MS.gene38976:intron
GTTACCACTGTTAATTCAAT+TGG 0.337932 8.4:+49127891 MS.gene38976:CDS
TATATCACCTGATAATTTGT+TGG 0.346753 8.4:+49127869 MS.gene38976:CDS
TTCAATAGAGAATAAATATA+TGG 0.355541 8.4:-49127813 None:intergenic
TTTAGGAGAATTATGAGAAA+TGG 0.365995 8.4:+49127765 MS.gene38976:CDS
TGAACTGTTTGATCTGTTTG+TGG 0.376105 8.4:+49127832 MS.gene38976:CDS
TAGGTAATATCTTTGCATTT+GGG 0.393033 8.4:+49127624 MS.gene38976:intron
TTGATTTGTTAGGTACCAAA+TGG 0.416862 8.4:+49128562 MS.gene38976:intron
AAAGATGCAGCTGGAATTGC+TGG 0.429368 8.4:+49127506 MS.gene38976:CDS
AAATGGGATAGGTACTGTTC+TGG 0.436030 8.4:+49128579 MS.gene38976:CDS
ATCTTTACCAATTTCACAAA+TGG 0.440430 8.4:-49127489 None:intergenic
GTGGTAACCAACAAATTATC+AGG 0.453364 8.4:-49127876 None:intergenic
TGTTTGATCTGTTTGTGGTA+TGG 0.469548 8.4:+49127837 MS.gene38976:CDS
GAGAATGTTTGGATTATTGA+TGG 0.473125 8.4:+49128075 MS.gene38976:CDS
GGATCAACGGAAATGTTCTC+AGG 0.474813 8.4:+49127786 MS.gene38976:CDS
TCTATGCGCCGAATGTTAGT+TGG 0.491353 8.4:+49128154 MS.gene38976:CDS
TCTCATAATTCTCCTAAATG+TGG 0.499347 8.4:-49127760 None:intergenic
GTAGAAAGCGAAAGATAAAA+CGG 0.499503 8.4:-49128369 None:intergenic
AATGGGATAGGTACTGTTCT+GGG 0.502068 8.4:+49128580 MS.gene38976:CDS
AGAACAGTACCTATCCCATT+TGG 0.512492 8.4:-49128577 None:intergenic
TGATTTGTTAGGTACCAAAT+GGG 0.514371 8.4:+49128563 MS.gene38976:intron
AAATTATCAGGTGATATAAG+AGG 0.515068 8.4:-49127864 None:intergenic
GAAGGAGATACTCATTAAGA+AGG 0.515628 8.4:-49128391 None:intergenic
CTCATAATTCTCCTAAATGT+GGG 0.519009 8.4:-49127759 None:intergenic
TCAAGCATCCAACTAACATT+CGG 0.535361 8.4:-49128162 None:intergenic
TATTTGTGATCATACTAGTC+GGG 0.546736 8.4:+49128110 MS.gene38976:CDS
TAGTAATCCATAAAGAAAGA+AGG 0.551073 8.4:-49128409 None:intergenic
AGTCGGGAGCTTGAAAATAA+CGG 0.564636 8.4:+49128126 MS.gene38976:CDS
TTATCCATACGACACTATCA+AGG 0.565472 8.4:-49128665 None:intergenic
TGTTAGGTACCAAATGGGAT+AGG 0.566157 8.4:+49128568 MS.gene38976:intron
GAAATTGGTAAAGATGCAGC+TGG 0.580425 8.4:+49127497 MS.gene38976:CDS
GAACCCTTGATAGTGTCGTA+TGG 0.586423 8.4:+49128661 MS.gene38976:CDS
ATATTTGTGATCATACTAGT+CGG 0.588397 8.4:+49128109 MS.gene38976:CDS
TACAATTATAAACAATGGAG+AGG 0.595947 8.4:-49128212 None:intergenic
AAACTTACAATTATAAACAA+TGG 0.596709 8.4:-49128217 None:intergenic
AATTATGAGAAATGGATCAA+CGG 0.620127 8.4:+49127773 MS.gene38976:CDS
TATCCATACGACACTATCAA+GGG 0.638241 8.4:-49128664 None:intergenic
TGCTCCAATTGAATTAACAG+TGG 0.678257 8.4:-49127895 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAATACTTACAT+TGG - chr8.4:49127662-49127681 None:intergenic 10.0%
!!! ATATCTTTTGATTATTAATT+TGG + chr8.4:49127716-49127735 MS.gene38976:intron 10.0%
!!! TATTTTATACTTTTTTTGAT+AGG + chr8.4:49128051-49128070 MS.gene38976:intron 10.0%
!!! TATTTTTCTAATACTATATA+TGG + chr8.4:49127980-49127999 MS.gene38976:intron 10.0%
!! AAACTTACAATTATAAACAA+TGG - chr8.4:49128220-49128239 None:intergenic 15.0%
!! ATAAAATAACTGAAAATTAG+AGG - chr8.4:49127546-49127565 None:intergenic 15.0%
!! TTCAATAGAGAATAAATATA+TGG - chr8.4:49127816-49127835 None:intergenic 15.0%
!! CTATATAATTTCACAGAAAT+TGG + chr8.4:49128317-49128336 MS.gene38976:intron 20.0%
!!! AGAAAAATAACAACCTTTTT+TGG - chr8.4:49127969-49127988 None:intergenic 20.0%
!!! GAATTCAGATTTTATTTTGT+TGG + chr8.4:49128511-49128530 MS.gene38976:intron 20.0%
! AAATTATCAGGTGATATAAG+AGG - chr8.4:49127867-49127886 None:intergenic 25.0%
! AATTATGAGAAATGGATCAA+CGG + chr8.4:49127773-49127792 MS.gene38976:CDS 25.0%
! ACAAATCAAACACAAAATGT+AGG - chr8.4:49128551-49128570 None:intergenic 25.0%
! ATATTTGTGATCATACTAGT+CGG + chr8.4:49128109-49128128 MS.gene38976:CDS 25.0%
! ATCTTTACCAATTTCACAAA+TGG - chr8.4:49127492-49127511 None:intergenic 25.0%
! ATTCTTGCTTGTTTATTTGT+AGG + chr8.4:49127605-49127624 MS.gene38976:intron 25.0%
! TACAATTATAAACAATGGAG+AGG - chr8.4:49128215-49128234 None:intergenic 25.0%
! TAGGTAATATCTTTGCATTT+GGG + chr8.4:49127624-49127643 MS.gene38976:intron 25.0%
! TAGTAAAACTCAGTATTACT+TGG - chr8.4:49128260-49128279 None:intergenic 25.0%
! TAGTAATCCATAAAGAAAGA+AGG - chr8.4:49128412-49128431 None:intergenic 25.0%
! TTTAGGAGAATTATGAGAAA+TGG + chr8.4:49127765-49127784 MS.gene38976:CDS 25.0%
!! TATATCACCTGATAATTTGT+TGG + chr8.4:49127869-49127888 MS.gene38976:CDS 25.0%
!!! CATTTTGTGTTTGATTTGTT+AGG + chr8.4:49128552-49128571 MS.gene38976:intron 25.0%
AGTATCTCCTTCTTTCTTTA+TGG + chr8.4:49128402-49128421 MS.gene38976:CDS 30.0%
CTCATAATTCTCCTAAATGT+GGG - chr8.4:49127762-49127781 None:intergenic 30.0%
GAGAATGTTTGGATTATTGA+TGG + chr8.4:49128075-49128094 MS.gene38976:CDS 30.0%
GTAGAAAGCGAAAGATAAAA+CGG - chr8.4:49128372-49128391 None:intergenic 30.0%
GTAGGTAATATCTTTGCATT+TGG + chr8.4:49127623-49127642 MS.gene38976:intron 30.0%
GTTACCACTGTTAATTCAAT+TGG + chr8.4:49127891-49127910 MS.gene38976:CDS 30.0%
TATTTGTGATCATACTAGTC+GGG + chr8.4:49128110-49128129 MS.gene38976:CDS 30.0%
TCTCATAATTCTCCTAAATG+TGG - chr8.4:49127763-49127782 None:intergenic 30.0%
! TTTTGATAGGTGAGAATGTT+TGG + chr8.4:49128064-49128083 MS.gene38976:intron 30.0%
!! TGATTTGTTAGGTACCAAAT+GGG + chr8.4:49128563-49128582 MS.gene38976:intron 30.0%
!! TTGATTTGTTAGGTACCAAA+TGG + chr8.4:49128562-49128581 MS.gene38976:intron 30.0%
AGTTTGTGTGAATTCAAGCT+TGG + chr8.4:49128279-49128298 MS.gene38976:intron 35.0%
CTATGCTGAGAAACCAAAAA+AGG + chr8.4:49127953-49127972 MS.gene38976:CDS 35.0%
GAAGGAGATACTCATTAAGA+AGG - chr8.4:49128394-49128413 None:intergenic 35.0%
GCTTACTCCATTTGTGAAAT+TGG + chr8.4:49127482-49127501 MS.gene38976:CDS 35.0%
GTGGTAACCAACAAATTATC+AGG - chr8.4:49127879-49127898 None:intergenic 35.0%
TATCCATACGACACTATCAA+GGG - chr8.4:49128667-49128686 None:intergenic 35.0%
TCAAGCATCCAACTAACATT+CGG - chr8.4:49128165-49128184 None:intergenic 35.0%
TGAACTGTTTGATCTGTTTG+TGG + chr8.4:49127832-49127851 MS.gene38976:CDS 35.0%
TGAATTGTAGACCCACATTT+AGG + chr8.4:49127748-49127767 MS.gene38976:intron 35.0%
TGCTCCAATTGAATTAACAG+TGG - chr8.4:49127898-49127917 None:intergenic 35.0%
TGTTTGATCTGTTTGTGGTA+TGG + chr8.4:49127837-49127856 MS.gene38976:CDS 35.0%
TTATCCATACGACACTATCA+AGG - chr8.4:49128668-49128687 None:intergenic 35.0%
!! GGATTATTGATGGCAGTTTT+TGG + chr8.4:49128085-49128104 MS.gene38976:CDS 35.0%
AGAACAGTACCTATCCCATT+TGG - chr8.4:49128580-49128599 None:intergenic 40.0%
AGTCGGGAGCTTGAAAATAA+CGG + chr8.4:49128126-49128145 MS.gene38976:CDS 40.0%
GAAATTGGTAAAGATGCAGC+TGG + chr8.4:49127497-49127516 MS.gene38976:CDS 40.0%
TGTTAGGTACCAAATGGGAT+AGG + chr8.4:49128568-49128587 MS.gene38976:intron 40.0%
! AAATGGGATAGGTACTGTTC+TGG + chr8.4:49128579-49128598 MS.gene38976:CDS 40.0%
! AATGGGATAGGTACTGTTCT+GGG + chr8.4:49128580-49128599 MS.gene38976:CDS 40.0%
AAAGATGCAGCTGGAATTGC+TGG + chr8.4:49127506-49127525 MS.gene38976:CDS 45.0%
GGATCAACGGAAATGTTCTC+AGG + chr8.4:49127786-49127805 MS.gene38976:CDS 45.0%
TCTATGCGCCGAATGTTAGT+TGG + chr8.4:49128154-49128173 MS.gene38976:CDS 45.0%
!! GAACCCTTGATAGTGTCGTA+TGG + chr8.4:49128661-49128680 MS.gene38976:CDS 45.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 49127467 49128687 49127467 ID=MS.gene38976
chr8.4 mRNA 49127467 49128687 49127467 ID=MS.gene38976.t1;Parent=MS.gene38976
chr8.4 exon 49127467 49127527 49127467 ID=MS.gene38976.t1.exon1;Parent=MS.gene38976.t1
chr8.4 CDS 49127467 49127527 49127467 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
chr8.4 exon 49127627 49127663 49127627 ID=MS.gene38976.t1.exon2;Parent=MS.gene38976.t1
chr8.4 CDS 49127627 49127663 49127627 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
chr8.4 exon 49127758 49127974 49127758 ID=MS.gene38976.t1.exon3;Parent=MS.gene38976.t1
chr8.4 CDS 49127758 49127974 49127758 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
chr8.4 exon 49128073 49128231 49128073 ID=MS.gene38976.t1.exon4;Parent=MS.gene38976.t1
chr8.4 CDS 49128073 49128231 49128073 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
chr8.4 exon 49128333 49128452 49128333 ID=MS.gene38976.t1.exon5;Parent=MS.gene38976.t1
chr8.4 CDS 49128333 49128452 49128333 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
chr8.4 exon 49128574 49128687 49128574 ID=MS.gene38976.t1.exon6;Parent=MS.gene38976.t1
chr8.4 CDS 49128574 49128687 49128574 ID=cds.MS.gene38976.t1;Parent=MS.gene38976.t1
Gene Sequence

>MS.gene38976

ATGTCTATGTTTAATGCTTACTCCATTTGTGAAATTGGTAAAGATGCAGCTGGAATTGCTGGTACTACATTTTCTTCCTCTAATTTTCAGTTATTTTATTACATTTAACATTTTTAACTTATCTTTATCTTGATCTCAATTCTTGCTTGTTTATTTGTAGGTAATATCTTTGCATTTGGGTTATTTCTATCTCCAATGTAAGTATTTTATTTATTAAATTAAAAATTAAGCTTAGAAAAATTTTCATGTATATCTTTTGATTATTAATTTGGATTATGTTGTGAATTGTAGACCCACATTTAGGAGAATTATGAGAAATGGATCAACGGAAATGTTCTCAGGATTGCCATATATTTATTCTCTATTGAACTGTTTGATCTGTTTGTGGTATGGTACTCCTCTTATATCACCTGATAATTTGTTGGTTACCACTGTTAATTCAATTGGAGCAGCTTTTCAGCTTGTGTACATTTTCTTATTCTTAATCTATGCTGAGAAACCAAAAAAGGTTGTTATTTTTCTAATACTATATATGGAAAGTATGATAAATTTCACATATATTTTGAAGTCTTACTCAGATGTGTTATTTTATACTTTTTTTGATAGGTGAGAATGTTTGGATTATTGATGGCAGTTTTTGGTATATTTGTGATCATACTAGTCGGGAGCTTGAAAATAACGGATAGTTCTATGCGCCGAATGTTAGTTGGATGCTTGAGTTGTGCTTCTCTCATTTCAATGTTTGCCTCTCCATTGTTTATAATTGTAAGTTTGACATTTTGCTAGATATCCAAGTAATACTGAGTTTTACTAGTTTGTGTGAATTCAAGCTTGGTGAAATTATTTATATCTATATAATTTCACAGAAATTGGTCATTCGCACAAAGAGTGTCGAGTTTATGCCGTTTTATCTTTCGCTTTCTACCTTCTTAATGAGTATCTCCTTCTTTCTTTATGGATTACTAAGTGATGATGTCTTCATTTATGTAAGAGTCTTTTCATCTCATTTCTCTGTTTCTTTCTGAAATATTGATAACAGTTTTAGAATTCAGATTTTATTTTGTTGGCTTATATTTGTATACCTACATTTTGTGTTTGATTTGTTAGGTACCAAATGGGATAGGTACTGTTCTGGGAATGATACAATTGATTTTATACTTCTATTACAAGAGTTCATCTAGCGATGACTCTACAGAACCCTTGATAGTGTCGTATGGATAA

Protein sequence

>MS.gene38976.t1

MSMFNAYSICEIGKDAAGIAGNIFAFGLFLSPIPTFRRIMRNGSTEMFSGLPYIYSLLNCLICLWYGTPLISPDNLLVTTVNSIGAAFQLVYIFLFLIYAEKPKKVRMFGLLMAVFGIFVIILVGSLKITDSSMRRMLVGCLSCASLISMFASPLFIIKLVIRTKSVEFMPFYLSLSTFLMSISFFLYGLLSDDVFIYVPNGIGTVLGMIQLILYFYYKSSSSDDSTEPLIVSYG