Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51643.t1 | XP_003598276.1 | 92.6 | 526 | 31 | 1 | 1 | 518 | 1 | 526 | 4.90E-281 | 976.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51643.t1 | Q9SUC6 | 53.8 | 533 | 223 | 9 | 1 | 514 | 1 | 529 | 2.5e-164 | 580.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51643.t1 | G7J0C2 | 92.6 | 526 | 31 | 1 | 1 | 518 | 1 | 526 | 3.5e-281 | 976.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028369 | MS.gene51643 | PPI |
| MS.gene35083 | MS.gene51643 | PPI |
| MS.gene028353 | MS.gene51643 | PPI |
| MS.gene28725 | MS.gene51643 | PPI |
| MS.gene35106 | MS.gene51643 | PPI |
| MS.gene36439 | MS.gene51643 | PPI |
| MS.gene28727 | MS.gene51643 | PPI |
| MS.gene35104 | MS.gene51643 | PPI |
| MS.gene51643 | MS.gene36444 | PPI |
| MS.gene35105 | MS.gene51643 | PPI |
| MS.gene36438 | MS.gene51643 | PPI |
| MS.gene003357 | MS.gene51643 | PPI |
| MS.gene28724 | MS.gene51643 | PPI |
| MS.gene51643 | MS.gene36437 | PPI |
| MS.gene028347 | MS.gene51643 | PPI |
| MS.gene02146 | MS.gene51643 | PPI |
| MS.gene028357 | MS.gene51643 | PPI |
| MS.gene51643 | MS.gene36435 | PPI |
| MS.gene35113 | MS.gene51643 | PPI |
| MS.gene36421 | MS.gene51643 | PPI |
| MS.gene51643 | MS.gene35103 | PPI |
| MS.gene28726 | MS.gene51643 | PPI |
| MS.gene51643 | MS.gene028346 | PPI |
| MS.gene28717 | MS.gene51643 | PPI |
| MS.gene28723 | MS.gene51643 | PPI |
| MS.gene35114 | MS.gene51643 | PPI |
| MS.gene028352 | MS.gene51643 | PPI |
| MS.gene35102 | MS.gene51643 | PPI |
| MS.gene028354 | MS.gene51643 | PPI |
| MS.gene28742 | MS.gene51643 | PPI |
| MS.gene047977 | MS.gene51643 | PPI |
| MS.gene36445 | MS.gene51643 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51643.t1 | MTR_3g009760 | 92.586 | 526 | 31 | 1 | 1 | 518 | 1 | 526 | 0.0 | 984 |
| MS.gene51643.t1 | MTR_3g009820 | 92.586 | 526 | 31 | 1 | 1 | 518 | 1 | 526 | 0.0 | 982 |
| MS.gene51643.t1 | MTR_3g009880 | 85.905 | 525 | 64 | 2 | 1 | 517 | 1 | 523 | 0.0 | 904 |
| MS.gene51643.t1 | MTR_2g031390 | 52.446 | 511 | 224 | 7 | 19 | 514 | 23 | 529 | 0.0 | 560 |
| MS.gene51643.t1 | MTR_4g094488 | 54.373 | 526 | 215 | 8 | 6 | 512 | 16 | 535 | 0.0 | 555 |
| MS.gene51643.t1 | MTR_2g031460 | 54.389 | 524 | 219 | 8 | 1 | 511 | 8 | 524 | 0.0 | 552 |
| MS.gene51643.t1 | MTR_2g031420 | 55.114 | 528 | 217 | 9 | 1 | 515 | 8 | 528 | 0.0 | 552 |
| MS.gene51643.t1 | MTR_4g094492 | 53.409 | 528 | 223 | 8 | 8 | 516 | 15 | 538 | 0.0 | 541 |
| MS.gene51643.t1 | MTR_4g094495 | 53.244 | 524 | 225 | 6 | 7 | 512 | 17 | 538 | 0.0 | 540 |
| MS.gene51643.t1 | MTR_4g094520 | 50.568 | 528 | 242 | 9 | 1 | 514 | 10 | 532 | 0.0 | 538 |
| MS.gene51643.t1 | MTR_4g094478 | 52.008 | 523 | 233 | 6 | 8 | 514 | 15 | 535 | 0.0 | 537 |
| MS.gene51643.t1 | MTR_4g094518 | 54.921 | 508 | 208 | 9 | 23 | 514 | 28 | 530 | 0.0 | 537 |
| MS.gene51643.t1 | MTR_4g094418 | 57.110 | 436 | 169 | 5 | 98 | 515 | 1 | 436 | 0.0 | 523 |
| MS.gene51643.t1 | MTR_4g094480 | 49.533 | 535 | 245 | 7 | 1 | 512 | 1 | 533 | 0.0 | 521 |
| MS.gene51643.t1 | MTR_2g031560 | 48.282 | 524 | 255 | 6 | 4 | 514 | 3 | 523 | 0.0 | 521 |
| MS.gene51643.t1 | MTR_4g094498 | 51.703 | 499 | 228 | 5 | 28 | 516 | 41 | 536 | 2.08e-180 | 518 |
| MS.gene51643.t1 | MTR_2g031590 | 48.282 | 524 | 249 | 8 | 9 | 514 | 13 | 532 | 4.78e-176 | 506 |
| MS.gene51643.t1 | MTR_2g031510 | 45.205 | 511 | 258 | 7 | 21 | 514 | 35 | 540 | 1.27e-159 | 465 |
| MS.gene51643.t1 | MTR_2g031490 | 43.511 | 524 | 279 | 5 | 7 | 514 | 16 | 538 | 4.02e-158 | 461 |
| MS.gene51643.t1 | MTR_4g057990 | 45.261 | 517 | 272 | 4 | 6 | 512 | 5 | 520 | 5.99e-158 | 460 |
| MS.gene51643.t1 | MTR_4g091200 | 46.272 | 523 | 259 | 8 | 4 | 512 | 10 | 524 | 2.55e-157 | 458 |
| MS.gene51643.t1 | MTR_4g088355 | 46.394 | 513 | 247 | 8 | 24 | 516 | 31 | 535 | 3.13e-155 | 453 |
| MS.gene51643.t1 | MTR_4g091190 | 45.094 | 530 | 257 | 10 | 4 | 512 | 10 | 526 | 4.16e-155 | 453 |
| MS.gene51643.t1 | MTR_4g091150 | 47.638 | 508 | 245 | 10 | 24 | 514 | 37 | 540 | 3.65e-154 | 451 |
| MS.gene51643.t1 | MTR_6g017205 | 45.143 | 525 | 262 | 9 | 6 | 512 | 8 | 524 | 9.48e-154 | 450 |
| MS.gene51643.t1 | MTR_4g094515 | 45.677 | 532 | 266 | 8 | 4 | 516 | 625 | 1152 | 1.37e-152 | 466 |
| MS.gene51643.t1 | MTR_4g094515 | 45.785 | 522 | 257 | 10 | 10 | 512 | 24 | 538 | 3.09e-149 | 457 |
| MS.gene51643.t1 | MTR_3g009850 | 88.793 | 232 | 26 | 0 | 287 | 518 | 1 | 232 | 1.83e-152 | 435 |
| MS.gene51643.t1 | MTR_4g091170 | 46.681 | 452 | 211 | 6 | 73 | 512 | 107 | 540 | 1.84e-146 | 432 |
| MS.gene51643.t1 | MTR_2g023590 | 46.304 | 460 | 231 | 6 | 70 | 516 | 12 | 468 | 3.98e-140 | 412 |
| MS.gene51643.t1 | MTR_1g076830 | 42.105 | 475 | 256 | 8 | 54 | 511 | 60 | 532 | 2.87e-121 | 367 |
| MS.gene51643.t1 | MTR_2g031600 | 45.923 | 233 | 115 | 3 | 9 | 232 | 14 | 244 | 3.52e-62 | 209 |
| MS.gene51643.t1 | MTR_2g031600 | 53.797 | 158 | 70 | 2 | 358 | 514 | 245 | 400 | 5.58e-53 | 184 |
| MS.gene51643.t1 | MTR_2g084370 | 73.786 | 103 | 13 | 1 | 122 | 224 | 1 | 89 | 2.94e-45 | 154 |
| MS.gene51643.t1 | MTR_2g084405 | 76.471 | 85 | 10 | 2 | 122 | 196 | 1 | 85 | 3.07e-29 | 111 |
| MS.gene51643.t1 | MTR_8g062280 | 47.458 | 59 | 29 | 1 | 460 | 518 | 21 | 77 | 2.78e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51643.t1 | AT4G20860 | 59.560 | 455 | 172 | 5 | 70 | 514 | 77 | 529 | 0.0 | 560 |
| MS.gene51643.t1 | AT5G44360 | 53.891 | 514 | 202 | 12 | 23 | 514 | 46 | 546 | 0.0 | 540 |
| MS.gene51643.t1 | AT5G44360 | 53.891 | 514 | 202 | 12 | 23 | 514 | 30 | 530 | 0.0 | 540 |
| MS.gene51643.t1 | AT5G44400 | 53.119 | 529 | 225 | 13 | 4 | 512 | 9 | 534 | 0.0 | 532 |
| MS.gene51643.t1 | AT2G34790 | 49.905 | 529 | 229 | 10 | 7 | 512 | 13 | 528 | 0.0 | 521 |
| MS.gene51643.t1 | AT1G30760 | 51.866 | 509 | 220 | 10 | 21 | 512 | 30 | 530 | 0.0 | 521 |
| MS.gene51643.t1 | AT1G30760 | 51.866 | 509 | 220 | 10 | 21 | 512 | 48 | 548 | 0.0 | 521 |
| MS.gene51643.t1 | AT5G44390 | 51.823 | 521 | 228 | 13 | 13 | 514 | 24 | 540 | 1.79e-180 | 518 |
| MS.gene51643.t1 | AT5G44380 | 50.000 | 524 | 239 | 12 | 9 | 512 | 17 | 537 | 1.18e-164 | 478 |
| MS.gene51643.t1 | AT5G44380 | 50.000 | 524 | 239 | 12 | 9 | 512 | 57 | 577 | 3.85e-164 | 478 |
| MS.gene51643.t1 | AT5G44440 | 45.251 | 537 | 260 | 11 | 4 | 517 | 7 | 532 | 4.64e-164 | 476 |
| MS.gene51643.t1 | AT5G44440 | 45.251 | 537 | 260 | 11 | 4 | 517 | 77 | 602 | 5.01e-163 | 476 |
| MS.gene51643.t1 | AT1G26420 | 46.429 | 532 | 256 | 9 | 1 | 512 | 1 | 523 | 2.62e-161 | 469 |
| MS.gene51643.t1 | AT4G20820 | 46.124 | 516 | 257 | 9 | 8 | 512 | 19 | 524 | 3.60e-161 | 468 |
| MS.gene51643.t1 | AT5G44410 | 46.183 | 524 | 258 | 9 | 7 | 514 | 14 | 529 | 5.24e-157 | 458 |
| MS.gene51643.t1 | AT1G26380 | 46.476 | 525 | 256 | 8 | 7 | 514 | 9 | 525 | 6.37e-157 | 457 |
| MS.gene51643.t1 | AT1G26410 | 48.954 | 478 | 225 | 7 | 48 | 512 | 76 | 547 | 3.78e-156 | 456 |
| MS.gene51643.t1 | AT1G26390 | 48.643 | 479 | 227 | 7 | 48 | 512 | 52 | 525 | 2.50e-155 | 453 |
| MS.gene51643.t1 | AT1G26400 | 45.930 | 516 | 263 | 7 | 8 | 511 | 10 | 521 | 1.97e-152 | 446 |
| MS.gene51643.t1 | AT1G30710 | 43.238 | 525 | 274 | 9 | 8 | 516 | 14 | 530 | 2.31e-151 | 443 |
| MS.gene51643.t1 | AT1G30700 | 46.154 | 507 | 251 | 9 | 24 | 514 | 26 | 526 | 2.31e-150 | 441 |
| MS.gene51643.t1 | AT1G34575 | 42.453 | 530 | 268 | 12 | 4 | 513 | 11 | 523 | 1.88e-149 | 438 |
| MS.gene51643.t1 | AT1G11770 | 41.366 | 527 | 293 | 8 | 4 | 518 | 11 | 533 | 1.15e-146 | 432 |
| MS.gene51643.t1 | AT1G01980 | 43.403 | 523 | 272 | 10 | 15 | 518 | 21 | 538 | 1.73e-145 | 429 |
| MS.gene51643.t1 | AT1G30730 | 43.595 | 523 | 278 | 7 | 4 | 513 | 6 | 524 | 1.78e-145 | 428 |
| MS.gene51643.t1 | AT4G20800 | 46.921 | 471 | 234 | 6 | 60 | 517 | 61 | 528 | 1.81e-144 | 426 |
| MS.gene51643.t1 | AT4G20840 | 42.801 | 507 | 271 | 8 | 22 | 514 | 34 | 535 | 8.32e-144 | 424 |
| MS.gene51643.t1 | AT1G30740 | 43.050 | 518 | 278 | 8 | 11 | 514 | 14 | 528 | 3.98e-141 | 417 |
| MS.gene51643.t1 | AT1G30740 | 43.050 | 518 | 278 | 8 | 11 | 514 | 17 | 531 | 4.15e-141 | 417 |
| MS.gene51643.t1 | AT4G20830 | 43.444 | 511 | 272 | 8 | 19 | 516 | 32 | 538 | 2.29e-140 | 416 |
| MS.gene51643.t1 | AT4G20830 | 43.418 | 509 | 271 | 8 | 19 | 514 | 32 | 536 | 1.91e-139 | 414 |
| MS.gene51643.t1 | AT2G34810 | 42.360 | 517 | 280 | 7 | 15 | 514 | 24 | 539 | 3.54e-137 | 407 |
| MS.gene51643.t1 | AT1G30720 | 40.856 | 514 | 287 | 7 | 13 | 513 | 16 | 525 | 2.39e-136 | 405 |
Find 93 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGCATCAAGTACTTTA+AGG | 0.182488 | 3.4:+4761079 | MS.gene51643:CDS |
| AAGCTGGTTTAGAAGGTATT+TGG | 0.194170 | 3.4:+4760745 | MS.gene51643:CDS |
| GCTCACCTTATAGATGTAAA+TGG | 0.201621 | 3.4:+4760216 | MS.gene51643:CDS |
| AATGAGAGTTTCCCTGAATT+AGG | 0.214946 | 3.4:+4760561 | MS.gene51643:CDS |
| CACATTAGCATTACTTATAA+TGG | 0.248787 | 3.4:+4760788 | MS.gene51643:CDS |
| TTGTCTTGCAACCCTAATTC+AGG | 0.255332 | 3.4:-4760573 | None:intergenic |
| CATCCACTTTATATGCTTGT+TGG | 0.273212 | 3.4:-4760931 | None:intergenic |
| CGATGAATCTGCTTGGATTC+AGG | 0.282488 | 3.4:+4759347 | MS.gene51643:CDS |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.4:+4760291 | MS.gene51643:CDS |
| AAGTGGATGAGAATGCTTTA+TGG | 0.287403 | 3.4:+4760945 | MS.gene51643:CDS |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.4:+4761000 | MS.gene51643:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.4:-4759182 | None:intergenic |
| CTATTCAGCAAGAGTTCTAT+AGG | 0.301114 | 3.4:-4760660 | None:intergenic |
| CTTGATGAATTCAGCCATCT+TGG | 0.311842 | 3.4:-4759132 | None:intergenic |
| CAATACTTGTGGATACAATA+TGG | 0.349912 | 3.4:-4759091 | None:intergenic |
| AGAGAGAGTTAAACTTGGTT+TGG | 0.351945 | 3.4:-4760503 | None:intergenic |
| ATGAGAGTTTCCCTGAATTA+GGG | 0.357011 | 3.4:+4760562 | MS.gene51643:CDS |
| CACATCAGTGGAGGTGGCTT+TGG | 0.360702 | 3.4:+4760147 | MS.gene51643:CDS |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.4:-4760735 | None:intergenic |
| TCTAAAGCAAGTAAAGTTCA+TGG | 0.367121 | 3.4:+4759405 | MS.gene51643:CDS |
| GGTACTCAGAAGCAAGTGTT+TGG | 0.380263 | 3.4:+4761060 | MS.gene51643:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.4:+4759363 | MS.gene51643:CDS |
| TTACTTATAATGGAACCTTA+TGG | 0.388867 | 3.4:+4760798 | MS.gene51643:CDS |
| TTGTTCCAATGTTCTTTGAA+TGG | 0.398919 | 3.4:-4760373 | None:intergenic |
| TGGGGCATCAAGTACTTTAA+GGG | 0.402731 | 3.4:+4761080 | MS.gene51643:CDS |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.4:-4760741 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.4:+4760738 | MS.gene51643:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.4:-4760978 | None:intergenic |
| CTTGTACAACATACAATATA+TGG | 0.415255 | 3.4:+4760878 | MS.gene51643:CDS |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.4:+4761001 | MS.gene51643:CDS |
| CATATTGTATCCACAAGTAT+TGG | 0.430537 | 3.4:+4759092 | MS.gene51643:CDS |
| GTACTCAGAAGCAAGTGTTT+GGG | 0.433402 | 3.4:+4761061 | MS.gene51643:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.4:-4759295 | None:intergenic |
| TCATTCAGTCTTCCACCATA+AGG | 0.436662 | 3.4:-4760813 | None:intergenic |
| TGGAGGTGGCTTTGGTACCT+TGG | 0.438981 | 3.4:+4760155 | MS.gene51643:CDS |
| AAGTTTAACTCTCTCTTCCT+TGG | 0.446047 | 3.4:+4760510 | MS.gene51643:CDS |
| CTATGTATTGCCACCTATGA+AGG | 0.446403 | 3.4:-4760410 | None:intergenic |
| AGAATAGTTGCTCAAAATAG+TGG | 0.457619 | 3.4:+4760462 | MS.gene51643:CDS |
| TCATTCTTGCATGGAAGATA+AGG | 0.459271 | 3.4:+4760316 | MS.gene51643:CDS |
| CTCACCTTATAGATGTAAAT+GGG | 0.461407 | 3.4:+4760217 | MS.gene51643:CDS |
| AATTCTTGATAGAAAATCAA+TGG | 0.470027 | 3.4:+4760242 | MS.gene51643:CDS |
| TAAGGGAAACTTTAAGCGAT+TGG | 0.470910 | 3.4:+4761097 | MS.gene51643:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.4:+4761014 | MS.gene51643:CDS |
| TGTCTTGCAACCCTAATTCA+GGG | 0.475992 | 3.4:-4760572 | None:intergenic |
| AAGACTGCATTGAAATGAGT+TGG | 0.478707 | 3.4:+4760595 | MS.gene51643:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.4:-4759294 | None:intergenic |
| TTCAGAAAAGCTACTACCTA+TGG | 0.478918 | 3.4:-4759034 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.4:-4759257 | None:intergenic |
| TAAGTTGTTAATTCAGGGGA+GGG | 0.480193 | 3.4:-4761189 | None:intergenic |
| TATTCAGCAAGAGTTCTATA+GGG | 0.484064 | 3.4:-4760659 | None:intergenic |
| ATCTTGCCGATGAATCTGCT+TGG | 0.489037 | 3.4:+4759340 | MS.gene51643:CDS |
| GAGCAACCAAGGTCCTTCAT+AGG | 0.491433 | 3.4:+4760397 | MS.gene51643:CDS |
| TTCAGTGATTCCAATACTTG+TGG | 0.491607 | 3.4:-4759102 | None:intergenic |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.4:-4759181 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.4:-4760996 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.509234 | 3.4:+4760928 | MS.gene51643:CDS |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 3.4:+4760243 | MS.gene51643:CDS |
| GTGGCTTTGGTACCTTGGTA+AGG | 0.531498 | 3.4:+4760160 | MS.gene51643:CDS |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.4:+4759417 | MS.gene51643:CDS |
| ATGGTGAATACTGTAACAAT+AGG | 0.540272 | 3.4:-4760354 | None:intergenic |
| TCTTGCATGGAAGATAAGGT+TGG | 0.542256 | 3.4:+4760320 | MS.gene51643:CDS |
| ATGCAGTGCATGTTAACCAT+AGG | 0.544066 | 3.4:+4759018 | MS.gene51643:CDS |
| CACTGAAACAAAATCCAAGA+TGG | 0.547598 | 3.4:+4759118 | MS.gene51643:CDS |
| ATTCACCATTCAAAGAACAT+TGG | 0.547935 | 3.4:+4760368 | MS.gene51643:CDS |
| GGTGGTCACATCAGTGGAGG+TGG | 0.552995 | 3.4:+4760141 | MS.gene51643:intron |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.4:+4760729 | MS.gene51643:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.4:-4759177 | None:intergenic |
| ACATACAATATATGGTCAAG+TGG | 0.568366 | 3.4:+4760886 | MS.gene51643:CDS |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.4:+4759231 | MS.gene51643:CDS |
| CTGAATCCAAGCAGATTCAT+CGG | 0.574804 | 3.4:-4759346 | None:intergenic |
| GAATCTGCTTGGATTCAGGC+TGG | 0.577913 | 3.4:+4759351 | MS.gene51643:CDS |
| TTAAGTTGTTAATTCAGGGG+AGG | 0.578715 | 3.4:-4761190 | None:intergenic |
| AAGGAAGAGAGAGTTAAACT+TGG | 0.579262 | 3.4:-4760508 | None:intergenic |
| GGTACCTTGGTAAGGAAACA+TGG | 0.585139 | 3.4:+4760168 | MS.gene51643:CDS |
| GTGAATACTGTAACAATAGG+TGG | 0.587206 | 3.4:-4760351 | None:intergenic |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.4:+4759249 | MS.gene51643:CDS |
| CTTATAATGGAACCTTATGG+TGG | 0.592307 | 3.4:+4760801 | MS.gene51643:CDS |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.4:+4759224 | MS.gene51643:CDS |
| CATACAATATATGGTCAAGT+GGG | 0.600319 | 3.4:+4760887 | MS.gene51643:CDS |
| GCAACAAGTATGACAATACA+AGG | 0.601545 | 3.4:+4761039 | MS.gene51643:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.603811 | 3.4:+4761062 | MS.gene51643:CDS |
| GGAGTAGGTGGTCACATCAG+TGG | 0.610281 | 3.4:+4760135 | MS.gene51643:intron |
| TTGCCACCTATGAAGGACCT+TGG | 0.622093 | 3.4:-4760403 | None:intergenic |
| CAAGATTAATGTCGATCGAA+CGG | 0.627262 | 3.4:-4759323 | None:intergenic |
| CAACCAAGGTCCTTCATAGG+TGG | 0.629693 | 3.4:+4760400 | MS.gene51643:CDS |
| GCTCAAACTAATTACCTGCA+GGG | 0.638432 | 3.4:-4759431 | None:intergenic |
| ATTGGAACAAGGAGCAACCA+AGG | 0.639041 | 3.4:+4760386 | MS.gene51643:CDS |
| CAGGGGAGGGAATTCAGAAG+AGG | 0.643159 | 3.4:-4761176 | None:intergenic |
| GAAGGTGTCATAATGAGAAG+AGG | 0.648177 | 3.4:-4759159 | None:intergenic |
| TAGACCATGTTTCCTTACCA+AGG | 0.652475 | 3.4:-4760172 | None:intergenic |
| GTAGGTGGTCACATCAGTGG+AGG | 0.669019 | 3.4:+4760138 | MS.gene51643:intron |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.4:+4759234 | MS.gene51643:CDS |
| ATTCAAAGAACATTGGAACA+AGG | 0.701106 | 3.4:+4760375 | MS.gene51643:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAAAACTATAACAAA+CGG | + | chr3.4:4759930-4759949 | MS.gene51643:intron | 10.0% |
| !! | TTAATTTAATATGTAACAAA+CGG | - | chr3.4:4759656-4759675 | None:intergenic | 10.0% |
| !!! | TCATAATAATTTAGTTTTTT+TGG | + | chr3.4:4759759-4759778 | MS.gene51643:intron | 10.0% |
| !!! | AAAAATTGATTTTTTGTTTG+AGG | - | chr3.4:4759851-4759870 | None:intergenic | 15.0% |
| !!! | TTTTCTTAAAAAATAACCAA+CGG | + | chr3.4:4760005-4760024 | MS.gene51643:intron | 15.0% |
| !! | AATTCTTGATAGAAAATCAA+TGG | + | chr3.4:4760242-4760261 | MS.gene51643:CDS | 20.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | + | chr3.4:4760243-4760262 | MS.gene51643:CDS | 20.0% |
| !! | GATTAAAAAAAAAAAACCGT+TGG | - | chr3.4:4760024-4760043 | None:intergenic | 20.0% |
| !!! | ATCTAGTTTTGACTTTATTT+TGG | + | chr3.4:4759629-4759648 | MS.gene51643:intron | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.4:4759297-4759316 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.4:4760096-4760115 | MS.gene51643:intron | 25.0% |
| ! | CACATTAGCATTACTTATAA+TGG | + | chr3.4:4760788-4760807 | MS.gene51643:CDS | 25.0% |
| ! | CTTGTACAACATACAATATA+TGG | + | chr3.4:4760878-4760897 | MS.gene51643:CDS | 25.0% |
| !! | CTCTTTTACATAATCAGATT+TGG | - | chr3.4:4760715-4760734 | None:intergenic | 25.0% |
| !! | TTAAGTTATCTTTTTCTCCA+AGG | - | chr3.4:4760530-4760549 | None:intergenic | 25.0% |
| !! | TTACTTATAATGGAACCTTA+TGG | + | chr3.4:4760798-4760817 | MS.gene51643:CDS | 25.0% |
| !! | TTTTCCCATTTACATCTATA+AGG | - | chr3.4:4760224-4760243 | None:intergenic | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.4:4761014-4761033 | MS.gene51643:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.4:4759260-4759279 | None:intergenic | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.4:4760928-4760947 | MS.gene51643:CDS | 30.0% | |
| ACATACAATATATGGTCAAG+TGG | + | chr3.4:4760886-4760905 | MS.gene51643:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.4:4760462-4760481 | MS.gene51643:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.4:4761001-4761020 | MS.gene51643:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.4:4759207-4759226 | MS.gene51643:CDS | 30.0% | |
| ATGGTGAATACTGTAACAAT+AGG | - | chr3.4:4760357-4760376 | None:intergenic | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.4:4760375-4760394 | MS.gene51643:CDS | 30.0% | |
| ATTCACCATTCAAAGAACAT+TGG | + | chr3.4:4760368-4760387 | MS.gene51643:CDS | 30.0% | |
| CAATACTTGTGGATACAATA+TGG | - | chr3.4:4759094-4759113 | None:intergenic | 30.0% | |
| CATACAATATATGGTCAAGT+GGG | + | chr3.4:4760887-4760906 | MS.gene51643:CDS | 30.0% | |
| CATATTGTATCCACAAGTAT+TGG | + | chr3.4:4759092-4759111 | MS.gene51643:CDS | 30.0% | |
| CTCACCTTATAGATGTAAAT+GGG | + | chr3.4:4760217-4760236 | MS.gene51643:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.4:4759298-4759317 | None:intergenic | 30.0% | |
| TATTCAGCAAGAGTTCTATA+GGG | - | chr3.4:4760662-4760681 | None:intergenic | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.4:4759184-4759203 | None:intergenic | 30.0% | |
| TTGTTCCAATGTTCTTTGAA+TGG | - | chr3.4:4760376-4760395 | None:intergenic | 30.0% | |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.4:4759208-4759227 | None:intergenic | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.4:4761147-4761166 | None:intergenic | 30.0% |
| ! | TACAAGTTTCCTTTTCTATG+TGG | - | chr3.4:4760864-4760883 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.4:4761146-4761165 | None:intergenic | 30.0% |
| ! | TCTAAAGCAAGTAAAGTTCA+TGG | + | chr3.4:4759405-4759424 | MS.gene51643:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.4:4760857-4760876 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.4:4760858-4760877 | None:intergenic | 30.0% |
| !! | ACTTATTTCTATAAGCTCTC+TGG | + | chr3.4:4759574-4759593 | MS.gene51643:intron | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+AGG | + | chr3.4:4760621-4760640 | MS.gene51643:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.4:4760260-4760279 | MS.gene51643:CDS | 30.0% |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.4:4760595-4760614 | MS.gene51643:CDS | 35.0% | |
| AAGGAAGAGAGAGTTAAACT+TGG | - | chr3.4:4760511-4760530 | None:intergenic | 35.0% | |
| AAGTGGATGAGAATGCTTTA+TGG | + | chr3.4:4760945-4760964 | MS.gene51643:CDS | 35.0% | |
| AAGTTTAACTCTCTCTTCCT+TGG | + | chr3.4:4760510-4760529 | MS.gene51643:CDS | 35.0% | |
| AATGAGAGTTTCCCTGAATT+AGG | + | chr3.4:4760561-4760580 | MS.gene51643:CDS | 35.0% | |
| AGAGAGAGTTAAACTTGGTT+TGG | - | chr3.4:4760506-4760525 | None:intergenic | 35.0% | |
| ATGAGAGTTTCCCTGAATTA+GGG | + | chr3.4:4760562-4760581 | MS.gene51643:CDS | 35.0% | |
| CAAGATTAATGTCGATCGAA+CGG | - | chr3.4:4759326-4759345 | None:intergenic | 35.0% | |
| CACTGAAACAAAATCCAAGA+TGG | + | chr3.4:4759118-4759137 | MS.gene51643:CDS | 35.0% | |
| CATCCACTTTATATGCTTGT+TGG | - | chr3.4:4760934-4760953 | None:intergenic | 35.0% | |
| CTATTCAGCAAGAGTTCTAT+AGG | - | chr3.4:4760663-4760682 | None:intergenic | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.4:4759185-4759204 | None:intergenic | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.4:4761000-4761019 | MS.gene51643:CDS | 35.0% | |
| GCAACAAGTATGACAATACA+AGG | + | chr3.4:4761039-4761058 | MS.gene51643:CDS | 35.0% | |
| GCTCACCTTATAGATGTAAA+TGG | + | chr3.4:4760216-4760235 | MS.gene51643:CDS | 35.0% | |
| GTGAATACTGTAACAATAGG+TGG | - | chr3.4:4760354-4760373 | None:intergenic | 35.0% | |
| TAAGGGAAACTTTAAGCGAT+TGG | + | chr3.4:4761097-4761116 | MS.gene51643:CDS | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.4:4760999-4761018 | None:intergenic | 35.0% | |
| TCATTCTTGCATGGAAGATA+AGG | + | chr3.4:4760316-4760335 | MS.gene51643:CDS | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.4:4759180-4759199 | None:intergenic | 35.0% | |
| TTCAGAAAAGCTACTACCTA+TGG | - | chr3.4:4759037-4759056 | None:intergenic | 35.0% | |
| TTCAGTGATTCCAATACTTG+TGG | - | chr3.4:4759105-4759124 | None:intergenic | 35.0% | |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.4:4760852-4760871 | MS.gene51643:CDS | 35.0% |
| ! | CTTATAATGGAACCTTATGG+TGG | + | chr3.4:4760801-4760820 | MS.gene51643:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.4:4761148-4761167 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.4:4760745-4760764 | MS.gene51643:CDS | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.4:4760981-4761000 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.4:4761138-4761157 | MS.gene51643:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.4:4760259-4760278 | MS.gene51643:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.4:4760270-4760289 | MS.gene51643:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.4:4760307-4760326 | MS.gene51643:CDS | 35.0% |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.4:4760114-4760133 | MS.gene51643:intron | 40.0% | |
| AGCTCAAACTAATTACCTGC+AGG | - | chr3.4:4759435-4759454 | None:intergenic | 40.0% | |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.4:4760744-4760763 | None:intergenic | 40.0% | |
| ATGCAGTGCATGTTAACCAT+AGG | + | chr3.4:4759018-4759037 | MS.gene51643:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.4:4760113-4760132 | None:intergenic | 40.0% | |
| CTATGTATTGCCACCTATGA+AGG | - | chr3.4:4760413-4760432 | None:intergenic | 40.0% | |
| CTGAATCCAAGCAGATTCAT+CGG | - | chr3.4:4759349-4759368 | None:intergenic | 40.0% | |
| CTTGATGAATTCAGCCATCT+TGG | - | chr3.4:4759135-4759154 | None:intergenic | 40.0% | |
| GCTCAAACTAATTACCTGCA+GGG | - | chr3.4:4759434-4759453 | None:intergenic | 40.0% | |
| GTACTCAGAAGCAAGTGTTT+GGG | + | chr3.4:4761061-4761080 | MS.gene51643:CDS | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.4:4761062-4761081 | MS.gene51643:CDS | 40.0% | |
| TAGACCATGTTTCCTTACCA+AGG | - | chr3.4:4760175-4760194 | None:intergenic | 40.0% | |
| TCATTCAGTCTTCCACCATA+AGG | - | chr3.4:4760816-4760835 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.4:4760738-4760757 | None:intergenic | 40.0% | |
| TCTTGCATGGAAGATAAGGT+TGG | + | chr3.4:4760320-4760339 | MS.gene51643:CDS | 40.0% | |
| TGTCTTGCAACCCTAATTCA+GGG | - | chr3.4:4760575-4760594 | None:intergenic | 40.0% | |
| TTGTCTTGCAACCCTAATTC+AGG | - | chr3.4:4760576-4760595 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.4:4760738-4760757 | MS.gene51643:CDS | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.4:4761080-4761099 | MS.gene51643:CDS | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.4:4761079-4761098 | MS.gene51643:CDS | 40.0% |
| !! | ATATGTCCAAGTGTTGGAGT+AGG | + | chr3.4:4760120-4760139 | MS.gene51643:intron | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.4:4759162-4759181 | None:intergenic | 40.0% |
| !!! | GGCTTTGAATGAGCTTTTGA+AGG | - | chr3.4:4760694-4760713 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.4:4760273-4760292 | MS.gene51643:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.4:4760729-4760748 | MS.gene51643:CDS | 45.0% | |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.4:4759417-4759436 | MS.gene51643:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.4:4759249-4759268 | MS.gene51643:CDS | 45.0% | |
| ATCTTGCCGATGAATCTGCT+TGG | + | chr3.4:4759340-4759359 | MS.gene51643:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.4:4759363-4759382 | MS.gene51643:CDS | 45.0% | |
| ATTGGAACAAGGAGCAACCA+AGG | + | chr3.4:4760386-4760405 | MS.gene51643:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.4:4759234-4759253 | MS.gene51643:CDS | 45.0% | |
| CGATGAATCTGCTTGGATTC+AGG | + | chr3.4:4759347-4759366 | MS.gene51643:CDS | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.4:4759224-4759243 | MS.gene51643:CDS | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.4:4760168-4760187 | MS.gene51643:CDS | 45.0% | |
| GGTACTCAGAAGCAAGTGTT+TGG | + | chr3.4:4761060-4761079 | MS.gene51643:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.4:4759231-4759250 | MS.gene51643:CDS | 45.0% | |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.4:4760291-4760310 | MS.gene51643:CDS | 45.0% |
| CAACCAAGGTCCTTCATAGG+TGG | + | chr3.4:4760400-4760419 | MS.gene51643:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.4:4759351-4759370 | MS.gene51643:CDS | 50.0% | |
| GAGCAACCAAGGTCCTTCAT+AGG | + | chr3.4:4760397-4760416 | MS.gene51643:CDS | 50.0% | |
| TGACCACCTACTCCAACACT+TGG | - | chr3.4:4760129-4760148 | None:intergenic | 50.0% | |
| TTGCCACCTATGAAGGACCT+TGG | - | chr3.4:4760406-4760425 | None:intergenic | 50.0% | |
| !! | GTGGCTTTGGTACCTTGGTA+AGG | + | chr3.4:4760160-4760179 | MS.gene51643:CDS | 50.0% |
| !! | TGTCCAAGTGTTGGAGTAGG+TGG | + | chr3.4:4760123-4760142 | MS.gene51643:intron | 50.0% |
| CACATCAGTGGAGGTGGCTT+TGG | + | chr3.4:4760147-4760166 | MS.gene51643:CDS | 55.0% | |
| CAGGGGAGGGAATTCAGAAG+AGG | - | chr3.4:4761179-4761198 | None:intergenic | 55.0% | |
| GGAGTAGGTGGTCACATCAG+TGG | + | chr3.4:4760135-4760154 | MS.gene51643:intron | 55.0% | |
| GTAGGTGGTCACATCAGTGG+AGG | + | chr3.4:4760138-4760157 | MS.gene51643:intron | 55.0% | |
| !! | TGGAGGTGGCTTTGGTACCT+TGG | + | chr3.4:4760155-4760174 | MS.gene51643:CDS | 55.0% |
| GGTGGTCACATCAGTGGAGG+TGG | + | chr3.4:4760141-4760160 | MS.gene51643:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 4758940 | 4761199 | 4758940 | ID=MS.gene51643 |
| chr3.4 | mRNA | 4758940 | 4761199 | 4758940 | ID=MS.gene51643.t1;Parent=MS.gene51643 |
| chr3.4 | exon | 4758940 | 4759438 | 4758940 | ID=MS.gene51643.t1.exon1;Parent=MS.gene51643.t1 |
| chr3.4 | CDS | 4758940 | 4759438 | 4758940 | ID=cds.MS.gene51643.t1;Parent=MS.gene51643.t1 |
| chr3.4 | exon | 4760142 | 4761199 | 4760142 | ID=MS.gene51643.t1.exon2;Parent=MS.gene51643.t1 |
| chr3.4 | CDS | 4760142 | 4761199 | 4760142 | ID=cds.MS.gene51643.t1;Parent=MS.gene51643.t1 |
| Gene Sequence |
| Protein sequence |
