AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35103

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35103.t1 MTR_4g094480 94.074 405 24 0 1 405 47 451 0.0 762
MS.gene35103.t1 MTR_4g094478 84.938 405 61 0 1 405 47 451 0.0 691
MS.gene35103.t1 MTR_4g094488 77.396 407 90 1 1 405 47 453 0.0 631
MS.gene35103.t1 MTR_4g094495 77.586 406 89 1 1 404 50 455 0.0 627
MS.gene35103.t1 MTR_4g094492 76.471 408 91 2 1 405 47 452 0.0 620
MS.gene35103.t1 MTR_4g094418 66.193 352 114 2 58 405 1 351 7.91e-168 484
MS.gene35103.t1 MTR_2g031560 51.378 399 190 2 5 403 43 437 7.21e-147 429
MS.gene35103.t1 MTR_2g031590 53.695 406 183 3 1 403 44 447 2.47e-144 421
MS.gene35103.t1 MTR_4g094498 54.839 403 176 3 1 403 53 449 3.72e-144 421
MS.gene35103.t1 MTR_2g031390 49.257 404 200 3 5 406 48 448 5.37e-143 418
MS.gene35103.t1 MTR_2g031420 54.613 401 176 5 2 401 48 443 3.32e-139 408
MS.gene35103.t1 MTR_3g009880 52.405 395 180 4 9 402 47 434 3.89e-139 408
MS.gene35103.t1 MTR_3g009820 51.538 390 182 3 13 402 54 436 1.72e-137 403
MS.gene35103.t1 MTR_4g094520 51.777 394 184 3 5 396 52 441 1.51e-136 401
MS.gene35103.t1 MTR_3g009760 51.026 390 184 3 13 402 54 436 3.63e-136 400
MS.gene35103.t1 MTR_2g031460 52.500 400 186 3 2 401 48 443 4.59e-136 400
MS.gene35103.t1 MTR_4g088355 50.617 405 195 3 2 405 49 449 2.92e-132 390
MS.gene35103.t1 MTR_4g091150 51.485 404 192 3 2 404 54 454 2.77e-131 388
MS.gene35103.t1 MTR_6g017205 50.493 406 196 3 1 405 41 442 3.41e-131 388
MS.gene35103.t1 MTR_4g094518 50.739 406 193 4 4 406 48 449 1.55e-130 386
MS.gene35103.t1 MTR_4g091200 49.507 406 200 3 1 405 41 442 2.97e-129 382
MS.gene35103.t1 MTR_4g091190 51.250 400 188 4 2 400 46 439 9.29e-129 381
MS.gene35103.t1 MTR_4g057990 50.124 403 192 4 1 402 41 435 1.32e-128 380
MS.gene35103.t1 MTR_2g031510 50.868 403 192 4 1 400 53 452 1.98e-127 378
MS.gene35103.t1 MTR_2g031490 50.372 403 194 4 1 400 51 450 7.14e-127 377
MS.gene35103.t1 MTR_4g094515 51.478 406 194 2 1 406 666 1068 2.78e-125 389
MS.gene35103.t1 MTR_4g094515 50.367 409 196 4 1 406 54 458 1.05e-119 374
MS.gene35103.t1 MTR_2g023590 50.000 380 183 3 22 400 4 377 1.31e-116 348
MS.gene35103.t1 MTR_4g091170 46.113 373 180 4 27 399 101 452 1.35e-112 341
MS.gene35103.t1 MTR_1g076830 43.073 397 214 5 11 402 58 447 2.98e-98 303
MS.gene35103.t1 MTR_2g031600 62.000 200 76 0 1 200 45 244 1.83e-75 240
MS.gene35103.t1 MTR_2g031600 45.205 73 38 1 331 403 245 315 6.38e-15 76.3
MS.gene35103.t1 MTR_3g009850 52.518 139 65 1 264 402 5 142 4.91e-41 145
MS.gene35103.t1 MTR_2g084370 45.045 111 39 2 82 192 1 89 1.20e-26 102
MS.gene35103.t1 MTR_2g084405 51.250 80 39 0 82 161 1 80 1.38e-17 78.2
MS.gene35103.t1 MTR_1g076760 83.333 42 6 1 215 255 33 74 3.50e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35103.t1 AT5G44440 52.369 401 182 3 3 402 47 439 3.21e-138 405
MS.gene35103.t1 AT5G44440 52.369 401 182 3 3 402 117 509 2.23e-137 406
MS.gene35103.t1 AT4G20820 50.000 402 191 3 1 402 51 442 2.24e-134 395
MS.gene35103.t1 AT5G44410 50.493 406 187 4 2 406 50 442 3.77e-128 380
MS.gene35103.t1 AT2G34790 48.564 383 192 3 17 397 65 444 5.28e-127 377
MS.gene35103.t1 AT1G26410 51.459 377 177 4 21 396 88 459 3.28e-125 373
MS.gene35103.t1 AT5G44400 47.970 394 195 5 7 396 57 444 4.30e-125 372
MS.gene35103.t1 AT5G44360 51.832 382 168 7 21 397 85 455 4.94e-125 372
MS.gene35103.t1 AT5G44360 51.832 382 168 7 21 397 69 439 5.11e-125 372
MS.gene35103.t1 AT1G26420 49.614 389 189 5 9 396 53 435 2.93e-123 367
MS.gene35103.t1 AT1G26400 48.371 399 197 5 1 396 43 435 1.87e-122 365
MS.gene35103.t1 AT1G30710 47.420 407 199 5 5 406 52 448 2.66e-122 365
MS.gene35103.t1 AT1G30760 47.558 389 199 3 11 397 61 446 3.46e-122 365
MS.gene35103.t1 AT1G30760 47.558 389 199 3 11 397 79 464 4.24e-122 365
MS.gene35103.t1 AT1G26380 48.872 399 195 6 1 396 43 435 3.43e-121 362
MS.gene35103.t1 AT1G26390 49.468 376 188 1 21 396 64 437 9.91e-121 361
MS.gene35103.t1 AT5G44390 47.277 404 199 7 1 396 51 448 1.30e-119 358
MS.gene35103.t1 AT1G30700 48.010 402 202 4 1 400 42 438 2.41e-119 357
MS.gene35103.t1 AT4G20860 48.964 386 185 7 23 406 73 448 1.36e-118 355
MS.gene35103.t1 AT5G44380 49.751 402 190 6 1 396 52 447 1.85e-118 355
MS.gene35103.t1 AT5G44380 49.751 402 190 6 1 396 92 487 4.27e-118 355
MS.gene35103.t1 AT1G30730 47.044 406 210 3 1 405 43 444 5.16e-118 353
MS.gene35103.t1 AT1G34575 47.512 402 197 5 6 403 50 441 1.57e-117 352
MS.gene35103.t1 AT1G11770 45.813 406 211 5 1 404 47 445 3.34e-116 349
MS.gene35103.t1 AT1G30720 47.901 405 206 3 2 405 45 445 7.54e-116 348
MS.gene35103.t1 AT4G20840 46.154 403 208 6 5 405 57 452 6.68e-114 343
MS.gene35103.t1 AT4G20800 44.472 398 214 4 1 396 43 435 7.36e-112 338
MS.gene35103.t1 AT2G34810 44.717 407 220 3 2 405 51 455 8.70e-107 325
MS.gene35103.t1 AT1G01980 44.526 411 214 7 1 404 47 450 3.46e-105 321
MS.gene35103.t1 AT4G20830 46.154 403 208 6 5 405 58 453 4.14e-104 318
MS.gene35103.t1 AT4G20830 46.154 403 208 6 5 405 58 453 9.24e-104 318
MS.gene35103.t1 AT1G30740 44.634 410 214 6 1 405 44 445 6.46e-102 313
MS.gene35103.t1 AT1G30740 44.634 410 214 6 1 405 47 448 8.53e-102 312

Find 83 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATGGTTGTTTGAATT+TGG 0.093676 4.4:-19916544 None:intergenic
GAATCGGTTCTTTACCTTTA+TGG 0.112377 4.4:+19917310 MS.gene35103:CDS
AGAATTCCCATTTCTGAAAT+TGG 0.145708 4.4:+19917418 MS.gene35103:CDS
GAATCTGCAGGCCGTGGTTT+TGG 0.227134 4.4:-19916571 None:intergenic
AAAATACACCATTGAATCTT+TGG 0.242615 4.4:-19917479 None:intergenic
GATTATGGTTGTTTGAATTT+GGG 0.274542 4.4:-19916543 None:intergenic
CCTTTGCAAGATAACATAAA+TGG 0.276498 4.4:-19917131 None:intergenic
TTCTAATCCAATTTCAGAAA+TGG 0.288639 4.4:-19917425 None:intergenic
ATTTCTGAAATTGGATTAGA+AGG 0.300676 4.4:+19917427 MS.gene35103:CDS
TTATTTCAAGCCTTGTTTCT+TGG 0.300694 4.4:+19917199 MS.gene35103:CDS
ATTCCATTTCCACATAGATA+TGG 0.327139 4.4:+19917544 MS.gene35103:CDS
CAATGGGTGAGGATTCCTTT+TGG 0.328365 4.4:+19916933 MS.gene35103:CDS
CGCCTTTCGGAAATCCATAA+AGG 0.332891 4.4:-19917324 None:intergenic
TTGAACACCGTCACACTTGA+TGG 0.340828 4.4:-19917025 None:intergenic
ATTTCAAGTGATTCGCCTTT+CGG 0.344771 4.4:-19917337 None:intergenic
AATGAACCGAGTACAGAATT+TGG 0.344869 4.4:-19917576 None:intergenic
GGTGGTGGAGGAGCAAGTTT+TGG 0.349746 4.4:+19916965 MS.gene35103:CDS
TCTCTAACCAAACCAAGTTC+TGG 0.358087 4.4:-19917262 None:intergenic
TGGCGTATGTTTCATGAAAA+TGG 0.372313 4.4:+19917454 MS.gene35103:CDS
TATTGATGAGTAAGAGGAAT+TGG 0.375876 4.4:-19916438 None:intergenic
TGAATTTGGGAAATTTGTTT+AGG 0.377551 4.4:-19916530 None:intergenic
CGAAGTCGTGGCTGCCGCTT+CGG 0.378058 4.4:-19916598 None:intergenic
TTTCCATATCTATGTGGAAA+TGG 0.380986 4.4:-19917547 None:intergenic
TCTAATCCAATTTCAGAAAT+GGG 0.386908 4.4:-19917424 None:intergenic
GGAAACTTGGTGCGTAAATT+CGG 0.389755 4.4:+19916842 MS.gene35103:CDS
AAGATTGTATTGAGATGAGT+TGG 0.395131 4.4:+19917284 MS.gene35103:CDS
AAATTCTGTACTCGGTTCAT+TGG 0.396326 4.4:+19917578 MS.gene35103:CDS
GTGATAATGACAAGAGGTTT+TGG 0.406236 4.4:-19916506 None:intergenic
AGATAAACTCATTCCCTTGA+TGG 0.407746 4.4:+19917228 MS.gene35103:CDS
CGGCAGGAAAACCAAGATTT+CGG 0.423578 4.4:-19916769 None:intergenic
GAAGTTTCCAGAACTTGGTT+TGG 0.432211 4.4:+19917255 MS.gene35103:CDS
TTTCAAGCCTTGTTTCTTGG+AGG 0.440275 4.4:+19917202 MS.gene35103:CDS
AGAACTACTTGGGTTCAATC+TGG 0.442862 4.4:+19916704 MS.gene35103:CDS
CACTTCAGTGGTGGTGGCTA+TGG 0.442973 4.4:+19916821 MS.gene35103:CDS
GAAGAGAAGTTTCCAGAACT+TGG 0.452732 4.4:+19917250 MS.gene35103:CDS
GTGAGATCAATGATGACAAA+TGG 0.455787 4.4:-19916647 None:intergenic
TGAGATGAGTTGGATTGAAT+CGG 0.456578 4.4:+19917294 MS.gene35103:CDS
CATTTATGTTATCTTGCAAA+GGG 0.465669 4.4:+19917132 MS.gene35103:CDS
TTGGAGTATTGATGAGTAAG+AGG 0.465775 4.4:-19916444 None:intergenic
TGGAAACTTCTCTTCCATCA+AGG 0.480434 4.4:-19917242 None:intergenic
TGGGCTATTAGAGGTGGTGG+AGG 0.487997 4.4:+19916953 MS.gene35103:CDS
TGTCCTACTGTAGGTGTTGG+TGG 0.493627 4.4:+19916797 MS.gene35103:CDS
CGCTTCGGATTCGAATCTGC+AGG 0.499907 4.4:-19916583 None:intergenic
GGAAACTTCTCTTCCATCAA+GGG 0.502745 4.4:-19917241 None:intergenic
TTATCCATTATGCCACCATA+AGG 0.506968 4.4:-19917505 None:intergenic
AAGCGGCAGCCACGACTTCG+AGG 0.514249 4.4:+19916601 MS.gene35103:CDS
ACCACCTCTAATAGCCCAAA+AGG 0.516876 4.4:-19916948 None:intergenic
CCATTTATGTTATCTTGCAA+AGG 0.517982 4.4:+19917131 MS.gene35103:CDS
GGATTCGAATCTGCAGGCCG+TGG 0.528579 4.4:-19916577 None:intergenic
TTTATACCAAATTCTGTACT+CGG 0.539802 4.4:+19917570 MS.gene35103:CDS
TATAAATTTCCATATCTATG+TGG 0.540221 4.4:-19917553 None:intergenic
TTTCTTGACAGACAAGCAAT+GGG 0.551117 4.4:+19916917 MS.gene35103:CDS
GTTTCTTGACAGACAAGCAA+TGG 0.552283 4.4:+19916916 MS.gene35103:CDS
TGAGATCAATGATGACAAAT+GGG 0.552570 4.4:-19916646 None:intergenic
CAACAGAACACAAGCTGCAA+AGG 0.553522 4.4:+19917366 MS.gene35103:CDS
AAATGGGACTTCAGAAACAT+AGG 0.554207 4.4:-19916630 None:intergenic
TGGTGGTGGCTATGGAAACT+TGG 0.558540 4.4:+19916829 MS.gene35103:CDS
TACCTTTATGGATTTCCGAA+AGG 0.562587 4.4:+19917322 MS.gene35103:CDS
AAATGGTGCCAAAGATTCAA+TGG 0.569099 4.4:+19917471 MS.gene35103:CDS
GGTGGACACTTCAGTGGTGG+TGG 0.569924 4.4:+19916815 MS.gene35103:CDS
ACCTACAGTAGGACAAGCAC+CGG 0.573133 4.4:-19916789 None:intergenic
CAACAAAGCTTGTTCACAAG+TGG 0.578289 4.4:+19917074 MS.gene35103:CDS
ACAGTAGGACAAGCACCGGC+AGG 0.585006 4.4:-19916785 None:intergenic
TGCTCACATAATTGATGTGA+AGG 0.585552 4.4:+19916889 MS.gene35103:CDS
GCATCAAGAAAGTGATGAGG+TGG 0.587589 4.4:+19917600 MS.gene35103:CDS
ATCTACACCTCCAAGAAACA+AGG 0.588375 4.4:-19917209 None:intergenic
ACATAATTGATGTGAAGGGT+AGG 0.589985 4.4:+19916894 MS.gene35103:CDS
TGACAGACAAGCAATGGGTG+AGG 0.595918 4.4:+19916922 MS.gene35103:CDS
GCCGGTGCTTGTCCTACTGT+AGG 0.597098 4.4:+19916788 MS.gene35103:CDS
GCTTGTCCTACTGTAGGTGT+TGG 0.601274 4.4:+19916794 MS.gene35103:CDS
AGGACCGACCCTCGAAGTCG+TGG 0.617522 4.4:-19916610 None:intergenic
AGCGGCAGCCACGACTTCGA+GGG 0.620087 4.4:+19916602 MS.gene35103:CDS
GTTGGTGGACACTTCAGTGG+TGG 0.625730 4.4:+19916812 MS.gene35103:CDS
GGTGTTGGTGGACACTTCAG+TGG 0.638405 4.4:+19916809 MS.gene35103:CDS
GCAGCCACGACTTCGAGGGT+CGG 0.640650 4.4:+19916606 MS.gene35103:CDS
TGAAGTTCCATCAAGTGTGA+CGG 0.645552 4.4:+19917018 MS.gene35103:CDS
TTAGGTGTGATAATGACAAG+AGG 0.654557 4.4:-19916512 None:intergenic
AAATTGGATTAGAAGGAATG+TGG 0.661238 4.4:+19917434 MS.gene35103:CDS
TGTCCACCAACACCTACAGT+AGG 0.663218 4.4:-19916800 None:intergenic
CTGCAGATTCGAATCCGAAG+CGG 0.676489 4.4:+19916584 MS.gene35103:CDS
ATAATCTGTTCCCAAAACCA+CGG 0.690765 4.4:+19916560 MS.gene35103:CDS
GCTCACATAATTGATGTGAA+GGG 0.694549 4.4:+19916890 MS.gene35103:CDS
TTGGCATCAAGAAAGTGATG+AGG 0.701714 4.4:+19917597 MS.gene35103:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAATTTCCATATCTATG+TGG - chr4.4:19917556-19917575 None:intergenic 20.0%
! AAAATACACCATTGAATCTT+TGG - chr4.4:19917482-19917501 None:intergenic 25.0%
! AGATTATGGTTGTTTGAATT+TGG - chr4.4:19916547-19916566 None:intergenic 25.0%
! AGGGTAAATTCAAGTAAAAA+AGG + chr4.4:19917151-19917170 MS.gene35103:CDS 25.0%
! ATGTAGAAAAAAGAACTACT+TGG + chr4.4:19916693-19916712 MS.gene35103:CDS 25.0%
! CATTTATGTTATCTTGCAAA+GGG + chr4.4:19917132-19917151 MS.gene35103:CDS 25.0%
! GATTATGGTTGTTTGAATTT+GGG - chr4.4:19916546-19916565 None:intergenic 25.0%
! TCTAATCCAATTTCAGAAAT+GGG - chr4.4:19917427-19917446 None:intergenic 25.0%
! TGTAGAAAAAAGAACTACTT+GGG + chr4.4:19916694-19916713 MS.gene35103:CDS 25.0%
! TTCTAATCCAATTTCAGAAA+TGG - chr4.4:19917428-19917447 None:intergenic 25.0%
! TTTATACCAAATTCTGTACT+CGG + chr4.4:19917570-19917589 MS.gene35103:CDS 25.0%
!! TGAATTTGGGAAATTTGTTT+AGG - chr4.4:19916533-19916552 None:intergenic 25.0%
!!! ATGGTGTATTTTTTTCCTTA+TGG + chr4.4:19917490-19917509 MS.gene35103:CDS 25.0%
!!! ATTTCTGAAATTGGATTAGA+AGG + chr4.4:19917427-19917446 MS.gene35103:CDS 25.0%
AAGATTGTATTGAGATGAGT+TGG + chr4.4:19917284-19917303 MS.gene35103:CDS 30.0%
ATTCCATTTCCACATAGATA+TGG + chr4.4:19917544-19917563 MS.gene35103:CDS 30.0%
CCATTTATGTTATCTTGCAA+AGG + chr4.4:19917131-19917150 MS.gene35103:CDS 30.0%
CCTTTGCAAGATAACATAAA+TGG - chr4.4:19917134-19917153 None:intergenic 30.0%
TATTGATGAGTAAGAGGAAT+TGG - chr4.4:19916441-19916460 None:intergenic 30.0%
TGAGATCAATGATGACAAAT+GGG - chr4.4:19916649-19916668 None:intergenic 30.0%
TTTCCATATCTATGTGGAAA+TGG - chr4.4:19917550-19917569 None:intergenic 30.0%
! AAATTGGATTAGAAGGAATG+TGG + chr4.4:19917434-19917453 MS.gene35103:CDS 30.0%
!! AGAATTCCCATTTCTGAAAT+TGG + chr4.4:19917418-19917437 MS.gene35103:CDS 30.0%
!! GCATTTTGATCCAATGTTTT+TGG - chr4.4:19917055-19917074 None:intergenic 30.0%
!! TGAGATTTTGAGTGGTAAAT+TGG - chr4.4:19916466-19916485 None:intergenic 30.0%
!! TTATTTCAAGCCTTGTTTCT+TGG + chr4.4:19917199-19917218 MS.gene35103:CDS 30.0%
!!! GTGTATTTTTTTCCTTATGG+TGG + chr4.4:19917493-19917512 MS.gene35103:CDS 30.0%
!!! GTTTTGGTGTCATAATTTCA+TGG + chr4.4:19916981-19917000 MS.gene35103:CDS 30.0%
AAATGGGACTTCAGAAACAT+AGG - chr4.4:19916633-19916652 None:intergenic 35.0%
AAATTCTGTACTCGGTTCAT+TGG + chr4.4:19917578-19917597 MS.gene35103:CDS 35.0%
AATGAACCGAGTACAGAATT+TGG - chr4.4:19917579-19917598 None:intergenic 35.0%
ACATAATTGATGTGAAGGGT+AGG + chr4.4:19916894-19916913 MS.gene35103:CDS 35.0%
AGATAAACTCATTCCCTTGA+TGG + chr4.4:19917228-19917247 MS.gene35103:CDS 35.0%
ATAATCTGTTCCCAAAACCA+CGG + chr4.4:19916560-19916579 MS.gene35103:CDS 35.0%
GCTCACATAATTGATGTGAA+GGG + chr4.4:19916890-19916909 MS.gene35103:CDS 35.0%
GTGAGATCAATGATGACAAA+TGG - chr4.4:19916650-19916669 None:intergenic 35.0%
GTTCAATGTGCCAAAAACAT+TGG + chr4.4:19917042-19917061 MS.gene35103:CDS 35.0%
TACCTTTATGGATTTCCGAA+AGG + chr4.4:19917322-19917341 MS.gene35103:CDS 35.0%
TGCTCACATAATTGATGTGA+AGG + chr4.4:19916889-19916908 MS.gene35103:CDS 35.0%
TGGCGTATGTTTCATGAAAA+TGG + chr4.4:19917454-19917473 MS.gene35103:CDS 35.0%
TTATCCATTATGCCACCATA+AGG - chr4.4:19917508-19917527 None:intergenic 35.0%
TTTCTTGACAGACAAGCAAT+GGG + chr4.4:19916917-19916936 MS.gene35103:CDS 35.0%
! AAATGGTGCCAAAGATTCAA+TGG + chr4.4:19917471-19917490 MS.gene35103:CDS 35.0%
! AACCAAGATTTCGGCTTTTT+CGG - chr4.4:19916763-19916782 None:intergenic 35.0%
! ATTTCAAGTGATTCGCCTTT+CGG - chr4.4:19917340-19917359 None:intergenic 35.0%
! GAATCGGTTCTTTACCTTTA+TGG + chr4.4:19917310-19917329 MS.gene35103:CDS 35.0%
! TGAGATGAGTTGGATTGAAT+CGG + chr4.4:19917294-19917313 MS.gene35103:CDS 35.0%
! TTAGGTGTGATAATGACAAG+AGG - chr4.4:19916515-19916534 None:intergenic 35.0%
! TTTTCCTTATGGTGGCATAA+TGG + chr4.4:19917501-19917520 MS.gene35103:CDS 35.0%
!! GTGATAATGACAAGAGGTTT+TGG - chr4.4:19916509-19916528 None:intergenic 35.0%
!! TGCAAATCTGAGATTTTGAG+TGG - chr4.4:19916474-19916493 None:intergenic 35.0%
!! TTGGAGTATTGATGAGTAAG+AGG - chr4.4:19916447-19916466 None:intergenic 35.0%
!!! AAGAGGTTTTGGTGTTGTTT+TGG - chr4.4:19916498-19916517 None:intergenic 35.0%
AGAACTACTTGGGTTCAATC+TGG + chr4.4:19916704-19916723 MS.gene35103:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG - chr4.4:19917212-19917231 None:intergenic 40.0%
GAAGAGAAGTTTCCAGAACT+TGG + chr4.4:19917250-19917269 MS.gene35103:CDS 40.0%
GAAGTTTCCAGAACTTGGTT+TGG + chr4.4:19917255-19917274 MS.gene35103:CDS 40.0%
GGAAACTTCTCTTCCATCAA+GGG - chr4.4:19917244-19917263 None:intergenic 40.0%
GTTTCTTGACAGACAAGCAA+TGG + chr4.4:19916916-19916935 MS.gene35103:CDS 40.0%
TCTCTAACCAAACCAAGTTC+TGG - chr4.4:19917265-19917284 None:intergenic 40.0%
TGAAGTTCCATCAAGTGTGA+CGG + chr4.4:19917018-19917037 MS.gene35103:CDS 40.0%
TGGAAACTTCTCTTCCATCA+AGG - chr4.4:19917245-19917264 None:intergenic 40.0%
TTGGCATCAAGAAAGTGATG+AGG + chr4.4:19917597-19917616 MS.gene35103:CDS 40.0%
! AATGGGTGAGGATTCCTTTT+GGG + chr4.4:19916934-19916953 MS.gene35103:CDS 40.0%
! CAACAAAGCTTGTTCACAAG+TGG + chr4.4:19917074-19917093 MS.gene35103:CDS 40.0%
! GATTCCTTTTGGGCTATTAG+AGG + chr4.4:19916944-19916963 MS.gene35103:CDS 40.0%
!! GGAAACTTGGTGCGTAAATT+CGG + chr4.4:19916842-19916861 MS.gene35103:CDS 40.0%
!! TTTCAAGCCTTGTTTCTTGG+AGG + chr4.4:19917202-19917221 MS.gene35103:CDS 40.0%
!!! GTGGTTTTGGGAACAGATTA+TGG - chr4.4:19916561-19916580 None:intergenic 40.0%
ACCACCTCTAATAGCCCAAA+AGG - chr4.4:19916951-19916970 None:intergenic 45.0%
AGCCGAAAAAGCCGAAATCT+TGG + chr4.4:19916758-19916777 MS.gene35103:CDS 45.0%
CAACAGAACACAAGCTGCAA+AGG + chr4.4:19917366-19917385 MS.gene35103:CDS 45.0%
CGCCTTTCGGAAATCCATAA+AGG - chr4.4:19917327-19917346 None:intergenic 45.0%
CGGCAGGAAAACCAAGATTT+CGG - chr4.4:19916772-19916791 None:intergenic 45.0%
GCATCAAGAAAGTGATGAGG+TGG + chr4.4:19917600-19917619 MS.gene35103:CDS 45.0%
TTGAACACCGTCACACTTGA+TGG - chr4.4:19917028-19917047 None:intergenic 45.0%
! CAATGGGTGAGGATTCCTTT+TGG + chr4.4:19916933-19916952 MS.gene35103:CDS 45.0%
! TCCTTTTGGGCTATTAGAGG+TGG + chr4.4:19916947-19916966 MS.gene35103:CDS 45.0%
! TTTTGGGCTATTAGAGGTGG+TGG + chr4.4:19916950-19916969 MS.gene35103:CDS 45.0%
!! CGAAATCTTGGTTTTCCTGC+CGG + chr4.4:19916770-19916789 MS.gene35103:CDS 45.0%
ACCTACAGTAGGACAAGCAC+CGG - chr4.4:19916792-19916811 None:intergenic 50.0%
CTGCAGATTCGAATCCGAAG+CGG + chr4.4:19916584-19916603 MS.gene35103:CDS 50.0%
GCTTGTCCTACTGTAGGTGT+TGG + chr4.4:19916794-19916813 MS.gene35103:CDS 50.0%
TGACAGACAAGCAATGGGTG+AGG + chr4.4:19916922-19916941 MS.gene35103:CDS 50.0%
TGTCCACCAACACCTACAGT+AGG - chr4.4:19916803-19916822 None:intergenic 50.0%
! AATCTGCAGGCCGTGGTTTT+GGG - chr4.4:19916573-19916592 None:intergenic 50.0%
!! TGGTGGTGGCTATGGAAACT+TGG + chr4.4:19916829-19916848 MS.gene35103:CDS 50.0%
!! TGTCCTACTGTAGGTGTTGG+TGG + chr4.4:19916797-19916816 MS.gene35103:CDS 50.0%
CGCTTCGGATTCGAATCTGC+AGG - chr4.4:19916586-19916605 None:intergenic 55.0%
! GAATCTGCAGGCCGTGGTTT+TGG - chr4.4:19916574-19916593 None:intergenic 55.0%
! GTTGGTGGACACTTCAGTGG+TGG + chr4.4:19916812-19916831 MS.gene35103:CDS 55.0%
! TGGGCTATTAGAGGTGGTGG+AGG + chr4.4:19916953-19916972 MS.gene35103:CDS 55.0%
!! CACTTCAGTGGTGGTGGCTA+TGG + chr4.4:19916821-19916840 MS.gene35103:CDS 55.0%
!! GGTGTTGGTGGACACTTCAG+TGG + chr4.4:19916809-19916828 MS.gene35103:CDS 55.0%
!!! GGTGGTGGAGGAGCAAGTTT+TGG + chr4.4:19916965-19916984 MS.gene35103:CDS 55.0%
GCCGGTGCTTGTCCTACTGT+AGG + chr4.4:19916788-19916807 MS.gene35103:CDS 60.0%
GGATTCGAATCTGCAGGCCG+TGG - chr4.4:19916580-19916599 None:intergenic 60.0%
GGTGGACACTTCAGTGGTGG+TGG + chr4.4:19916815-19916834 MS.gene35103:CDS 60.0%
!! ACAGTAGGACAAGCACCGGC+AGG - chr4.4:19916788-19916807 None:intergenic 60.0%
AAGCGGCAGCCACGACTTCG+AGG + chr4.4:19916601-19916620 MS.gene35103:CDS 65.0%
AGCGGCAGCCACGACTTCGA+GGG + chr4.4:19916602-19916621 MS.gene35103:CDS 65.0%
AGGACCGACCCTCGAAGTCG+TGG - chr4.4:19916613-19916632 None:intergenic 65.0%
GCAGCCACGACTTCGAGGGT+CGG + chr4.4:19916606-19916625 MS.gene35103:CDS 65.0%
! CGAAGTCGTGGCTGCCGCTT+CGG - chr4.4:19916601-19916620 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 19916413 19917633 19916413 ID=MS.gene35103
chr4.4 mRNA 19916413 19917633 19916413 ID=MS.gene35103.t1;Parent=MS.gene35103
chr4.4 exon 19916413 19917633 19916413 ID=MS.gene35103.t1.exon1;Parent=MS.gene35103.t1
chr4.4 CDS 19916413 19917633 19916413 ID=cds.MS.gene35103.t1;Parent=MS.gene35103.t1
Gene Sequence

>MS.gene35103

ATGTCTAAAGTTGTGTACACAAAAACCAATTCCTCTTACTCATCAATACTCCAATTTACCACTCAAAATCTCAGATTTGCATCCAAAACAACACCAAAACCTCTTGTCATTATCACACCTAAACAAATTTCCCAAATTCAAACAACCATAATCTGTTCCCAAAACCACGGCCTGCAGATTCGAATCCGAAGCGGCAGCCACGACTTCGAGGGTCGGTCCTATGTTTCTGAAGTCCCATTTGTCATCATTGATCTCACATACTTCAGAGAAATTACAGTACATGTAGAAAAAAGAACTACTTGGGTTCAATCTGGTGCAACACTTGATGAACTTTACTACACAATTAGCCGAAAAAGCCGAAATCTTGGTTTTCCTGCCGGTGCTTGTCCTACTGTAGGTGTTGGTGGACACTTCAGTGGTGGTGGCTATGGAAACTTGGTGCGTAAATTCGGTCTTGCAGCTGATAATATAATTGATGCTCACATAATTGATGTGAAGGGTAGGTTTCTTGACAGACAAGCAATGGGTGAGGATTCCTTTTGGGCTATTAGAGGTGGTGGAGGAGCAAGTTTTGGTGTCATAATTTCATGGAAGATTAAACTAGTTGAAGTTCCATCAAGTGTGACGGTGTTCAATGTGCCAAAAACATTGGATCAAAATGCAACAAAGCTTGTTCACAAGTGGCAGTTTGTGACAAGTACAATTGATGAAAACATAGCCATTTATGTTATCTTGCAAAGGGTAAATTCAAGTAAAAAAGGTAAGTCAAATTCAACAGTACAAGCTTTATTTCAAGCCTTGTTTCTTGGAGGTGTAGATAAACTCATTCCCTTGATGGAAGAGAAGTTTCCAGAACTTGGTTTGGTTAGAGAAGATTGTATTGAGATGAGTTGGATTGAATCGGTTCTTTACCTTTATGGATTTCCGAAAGGCGAATCACTTGAAATGTTGCTCAACAGAACACAAGCTGCAAAGGATATTTTCAAAATAAAGTCTGATTTTGTGAGAATTCCCATTTCTGAAATTGGATTAGAAGGAATGTGGCGTATGTTTCATGAAAATGGTGCCAAAGATTCAATGGTGTATTTTTTTCCTTATGGTGGCATAATGGATAATATTTCAGAGTCTGAAATTCCATTTCCACATAGATATGGAAATTTATACCAAATTCTGTACTCGGTTCATTGGCATCAAGAAAGTGATGAGGTGGAAAAAAATTAA
Protein sequence

>MS.gene35103.t1

MSKVVYTKTNSSYSSILQFTTQNLRFASKTTPKPLVIITPKQISQIQTTIICSQNHGLQIRIRSGSHDFEGRSYVSEVPFVIIDLTYFREITVHVEKRTTWVQSGATLDELYYTISRKSRNLGFPAGACPTVGVGGHFSGGGYGNLVRKFGLAADNIIDAHIIDVKGRFLDRQAMGEDSFWAIRGGGGASFGVIISWKIKLVEVPSSVTVFNVPKTLDQNATKLVHKWQFVTSTIDENIAIYVILQRVNSSKKGKSNSTVQALFQALFLGGVDKLIPLMEEKFPELGLVREDCIEMSWIESVLYLYGFPKGESLEMLLNRTQAAKDIFKIKSDFVRIPISEIGLEGMWRMFHENGAKDSMVYFFPYGGIMDNISESEIPFPHRYGNLYQILYSVHWHQESDEVEKN