AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35113.t1 MTR_4g094518 98.715 467 6 0 1 467 37 503 0.0 956
MS.gene35113.t1 MTR_2g031390 81.034 464 88 0 3 466 38 501 0.0 795
MS.gene35113.t1 MTR_4g094520 72.511 462 120 2 9 467 48 505 0.0 673
MS.gene35113.t1 MTR_3g009760 55.902 449 189 5 21 467 54 495 3.55e-178 509
MS.gene35113.t1 MTR_3g009820 55.679 449 190 5 21 467 54 495 3.35e-177 507
MS.gene35113.t1 MTR_4g094488 55.819 464 194 6 12 467 50 510 1.29e-176 506
MS.gene35113.t1 MTR_3g009880 54.803 458 197 6 12 467 44 493 1.32e-175 503
MS.gene35113.t1 MTR_4g094498 54.936 466 204 3 3 467 47 507 1.32e-175 504
MS.gene35113.t1 MTR_2g031590 51.277 470 222 4 2 466 37 504 6.15e-173 496
MS.gene35113.t1 MTR_2g031560 50.321 467 227 4 2 466 32 495 7.77e-173 497
MS.gene35113.t1 MTR_4g094492 53.978 465 200 7 12 467 50 509 5.00e-170 489
MS.gene35113.t1 MTR_2g031460 53.965 454 204 4 13 466 51 499 7.00e-170 489
MS.gene35113.t1 MTR_4g094495 54.113 462 203 5 13 467 54 513 1.98e-169 488
MS.gene35113.t1 MTR_2g031420 53.965 454 204 4 13 466 51 499 3.54e-168 484
MS.gene35113.t1 MTR_4g094478 53.463 462 206 6 12 467 50 508 1.42e-166 481
MS.gene35113.t1 MTR_4g094480 52.278 461 211 6 13 467 51 508 8.92e-163 471
MS.gene35113.t1 MTR_4g094418 54.768 409 175 5 66 466 1 407 3.33e-160 467
MS.gene35113.t1 MTR_4g091200 48.807 461 222 7 14 467 46 499 6.14e-153 445
MS.gene35113.t1 MTR_2g031490 48.932 468 231 4 2 465 46 509 7.80e-150 438
MS.gene35113.t1 MTR_6g017205 48.156 461 225 7 14 467 46 499 2.49e-149 437
MS.gene35113.t1 MTR_2g031510 48.796 457 228 3 13 465 57 511 9.27e-148 432
MS.gene35113.t1 MTR_4g088355 47.495 459 233 6 13 467 52 506 8.15e-147 430
MS.gene35113.t1 MTR_4g094515 48.352 455 230 4 13 465 670 1121 3.75e-146 447
MS.gene35113.t1 MTR_4g094515 48.359 457 229 4 13 465 58 511 7.06e-138 425
MS.gene35113.t1 MTR_4g057990 47.289 461 227 7 13 467 45 495 2.02e-144 423
MS.gene35113.t1 MTR_4g091150 47.579 475 233 9 2 467 46 513 3.40e-144 424
MS.gene35113.t1 MTR_4g091190 46.739 460 231 6 13 466 49 500 1.23e-143 422
MS.gene35113.t1 MTR_2g023590 45.578 441 230 6 31 467 5 439 4.51e-134 395
MS.gene35113.t1 MTR_4g091170 43.953 430 219 6 39 466 105 514 4.79e-134 399
MS.gene35113.t1 MTR_1g076830 39.112 473 278 5 1 465 36 506 1.58e-106 327
MS.gene35113.t1 MTR_2g031600 55.024 209 92 1 2 210 38 244 2.43e-73 237
MS.gene35113.t1 MTR_2g031600 50.781 128 62 1 340 466 245 372 2.17e-40 149
MS.gene35113.t1 MTR_3g009850 55.224 201 88 2 269 467 1 201 2.88e-71 226
MS.gene35113.t1 MTR_2g084370 44.144 111 40 2 92 202 1 89 1.62e-26 103
MS.gene35113.t1 MTR_2g084405 48.101 79 41 0 92 170 1 79 1.10e-17 79.0
MS.gene35113.t1 MTR_8g062280 49.315 73 9 1 395 467 4 48 1.66e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35113.t1 AT1G30760 65.812 468 154 2 2 467 60 523 0.0 633
MS.gene35113.t1 AT1G30760 65.812 468 154 2 2 467 42 505 0.0 632
MS.gene35113.t1 AT2G34790 64.316 468 161 2 2 467 40 503 0.0 619
MS.gene35113.t1 AT5G44400 63.830 470 162 5 2 466 42 508 0.0 595
MS.gene35113.t1 AT5G44390 59.746 472 180 6 2 466 44 512 0.0 553
MS.gene35113.t1 AT5G44380 59.023 471 185 5 2 467 85 552 0.0 527
MS.gene35113.t1 AT5G44380 59.023 471 185 5 2 467 45 512 0.0 525
MS.gene35113.t1 AT1G30700 50.641 468 226 4 2 467 35 499 3.28e-157 456
MS.gene35113.t1 AT4G20820 50.109 459 213 8 12 467 54 499 3.08e-152 444
MS.gene35113.t1 AT4G20860 51.748 429 199 6 41 467 80 502 1.25e-150 439
MS.gene35113.t1 AT5G44360 50.545 459 212 8 13 465 52 501 2.08e-150 439
MS.gene35113.t1 AT5G44360 50.545 459 212 8 13 465 68 517 2.60e-150 439
MS.gene35113.t1 AT4G20840 48.578 457 226 5 12 465 56 506 8.45e-148 432
MS.gene35113.t1 AT5G44440 47.679 474 231 8 1 467 39 502 1.77e-144 424
MS.gene35113.t1 AT5G44440 47.679 474 231 8 1 467 109 572 1.62e-143 424
MS.gene35113.t1 AT1G30710 47.312 465 228 8 8 466 47 500 2.15e-143 421
MS.gene35113.t1 AT5G44410 50.753 465 204 9 13 467 53 502 6.22e-143 420
MS.gene35113.t1 AT1G11770 48.330 449 223 6 20 465 58 500 1.23e-142 419
MS.gene35113.t1 AT1G34575 46.467 467 234 8 6 467 42 497 1.87e-142 419
MS.gene35113.t1 AT4G20800 46.855 461 240 4 8 467 42 498 7.33e-141 414
MS.gene35113.t1 AT1G26380 47.527 465 231 8 8 467 42 498 5.14e-140 412
MS.gene35113.t1 AT1G30730 47.033 455 234 5 14 466 48 497 1.80e-139 411
MS.gene35113.t1 AT1G30720 47.752 467 235 6 3 466 38 498 2.68e-138 408
MS.gene35113.t1 AT1G26390 47.368 456 234 4 14 467 49 500 1.93e-137 406
MS.gene35113.t1 AT1G26420 47.345 452 232 5 17 467 52 498 4.21e-137 405
MS.gene35113.t1 AT4G20830 47.265 457 232 5 12 465 57 507 1.43e-135 401
MS.gene35113.t1 AT1G26400 47.186 462 236 6 8 467 42 497 2.36e-135 400
MS.gene35113.t1 AT1G26410 46.799 453 233 6 17 467 76 522 3.60e-135 401
MS.gene35113.t1 AT4G20830 47.265 457 232 5 12 465 57 507 5.68e-135 401
MS.gene35113.t1 AT2G34810 45.666 473 245 7 3 467 44 512 3.29e-130 388
MS.gene35113.t1 AT1G01980 46.696 454 228 7 20 465 58 505 7.64e-130 387
MS.gene35113.t1 AT1G30740 46.563 451 230 7 20 465 58 502 1.84e-127 380
MS.gene35113.t1 AT1G30740 46.563 451 230 7 20 465 55 499 1.85e-127 380

Find 109 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGAAAGGTGTTTCGATTT+TGG 0.085027 4.4:+19833758 None:intergenic
CCACCTGAAGTTCTTCTTAA+AGG 0.167526 4.4:-19833045 MS.gene35113:CDS
CCATGGGAGAGGATCTATTT+TGG 0.198723 4.4:-19833458 MS.gene35113:CDS
GAAAACAAGTTTCCCTGAAT+TGG 0.232841 4.4:-19833145 MS.gene35113:CDS
GAAGCTTTGGTATACTTCTT+TGG 0.234046 4.4:-19833413 MS.gene35113:CDS
TTCCTTGTCAAACCCAATTC+AGG 0.239581 4.4:+19833132 None:intergenic
TACGAGAAAAGTTCGGTTCT+TGG 0.245296 4.4:-19833633 MS.gene35113:CDS
AAGCGACTGGTTGGGGTTAT+AGG 0.248483 4.4:-19832642 MS.gene35113:CDS
AAGATAGCCCTTTGATGATT+TGG 0.263280 4.4:-19832921 MS.gene35113:CDS
GGGAAGCTTATGTGAATTAT+AGG 0.274126 4.4:-19832711 MS.gene35113:CDS
GAATTATAGGGATCTTGATT+TGG 0.282140 4.4:-19832698 MS.gene35113:CDS
CACCACTTCTTACTCTTAGA+TGG 0.284899 4.4:+19833811 None:intergenic
AATTATAGGGATCTTGATTT+GGG 0.302116 4.4:-19832697 MS.gene35113:CDS
AATCCTTCAATACCAGTTTC+TGG 0.308973 4.4:+19832964 None:intergenic
AAATTCAGTACTTGTCTATA+TGG 0.320076 4.4:-19832822 MS.gene35113:CDS
GTGAATATGAATTCGGGTTT+CGG 0.327667 4.4:+19833891 None:intergenic
TCAATACCAGTTTCTGGTAT+TGG 0.333889 4.4:+19832970 None:intergenic
GTTGAGAATGAAGGTTCATT+TGG 0.337178 4.4:+19833963 None:intergenic
CAAAGCATGTAGACTGGATT+AGG 0.349139 4.4:-19832777 MS.gene35113:CDS
ACTGTTACTGTTGGTGGAAC+AGG 0.356751 4.4:+19833372 None:intergenic
GGAGGGTGTGAATATGAATT+CGG 0.357226 4.4:+19833884 None:intergenic
TAGATGGATTCCGAGTTTCT+TGG 0.364310 4.4:+19833827 None:intergenic
AAAACAAGTTTCCCTGAATT+GGG 0.364442 4.4:-19833144 MS.gene35113:CDS
GTTCCATTTCTATGTGGAAA+AGG 0.370940 4.4:+19832853 None:intergenic
CTGCTTGGACATGTGAATCT+TGG 0.374928 4.4:+19833862 None:intergenic
GGAACATTGATGAGAAAATA+TGG 0.375969 4.4:-19833549 MS.gene35113:CDS
AAATCTGTTCTCTATATTGC+TGG 0.377366 4.4:-19833084 MS.gene35113:CDS
CTTGGAAACTTTGACACATA+AGG 0.377974 4.4:+19832733 None:intergenic
GAGTTTCTTGGAGCAAATTA+CGG 0.395398 4.4:+19833839 None:intergenic
CTATATAGAAGCGACTGGTT+GGG 0.401524 4.4:-19832650 MS.gene35113:CDS
GAGGGTGTGAATATGAATTC+GGG 0.402046 4.4:+19833885 None:intergenic
GTGTAAATTGTTGTATGAAA+TGG 0.405051 4.4:+19833987 None:intergenic
TGCTTGGACATGTGAATCTT+GGG 0.407914 4.4:+19833863 None:intergenic
AATTCGGGTTTCGGTGTAGA+TGG 0.409131 4.4:+19833900 None:intergenic
GGTGGTGGAGGTGGAAGCTT+TGG 0.413015 4.4:-19833426 MS.gene35113:CDS
TTTCCTTTAAGAAGAACTTC+AGG 0.413371 4.4:+19833042 None:intergenic
AGCAATGAACCTAAGGTGTT+TGG 0.416350 4.4:-19833925 MS.gene35113:CDS
TCGGTTCTTGGTTACCCTGC+AGG 0.425653 4.4:-19833621 MS.gene35113:CDS
CACATAACAGGAGGTGCATA+TGG 0.426699 4.4:-19833570 MS.gene35113:CDS
GCTATAAGAGGTGGTGGAGG+TGG 0.427869 4.4:-19833435 MS.gene35113:CDS
GGAAGCTTATGTGAATTATA+GGG 0.435715 4.4:-19832710 MS.gene35113:CDS
AACACTGCTTGGGTTCAATC+TGG 0.438165 4.4:-19833687 MS.gene35113:CDS
ACACTAAAACCAACACTGCT+TGG 0.438629 4.4:-19833698 MS.gene35113:CDS
GCTCGAATCGTTGATGCAAA+TGG 0.448524 4.4:-19833501 MS.gene35113:CDS
TTCCATTTCTATGTGGAAAA+GGG 0.450370 4.4:+19832854 None:intergenic
CACTAAAACCAACACTGCTT+GGG 0.452600 4.4:-19833697 MS.gene35113:CDS
TCCTTGTCAAACCCAATTCA+GGG 0.453047 4.4:+19833133 None:intergenic
TATATAGAAGCGACTGGTTG+GGG 0.455293 4.4:-19832649 MS.gene35113:CDS
GCTATATAGAAGCGACTGGT+TGG 0.459836 4.4:-19832651 MS.gene35113:CDS
GGTGTCGGTGGACACATAAC+AGG 0.463630 4.4:-19833582 MS.gene35113:CDS
AAGATTGTCAAGAAACTAGT+TGG 0.464462 4.4:-19833110 MS.gene35113:CDS
TGGAGCAAATTACGGCTGCT+TGG 0.470001 4.4:+19833847 None:intergenic
AGGGAACCAATACCAGAAAC+TGG 0.473053 4.4:-19832976 MS.gene35113:CDS
AAGCTTGTGCATAGACCTGC+AGG 0.473568 4.4:+19833606 None:intergenic
GCAGGTCTATGCACAAGCTT+AGG 0.479193 4.4:-19833603 MS.gene35113:CDS
TTGATGATTTGGAATCCATA+TGG 0.487109 4.4:-19832910 MS.gene35113:CDS
GGTTCATTGCTAAGGAATCA+AGG 0.487996 4.4:+19833937 None:intergenic
GTAATAGACTTCACCGATAG+TGG 0.498495 4.4:+19833662 None:intergenic
TAATTCACATAAGCTTCCCT+TGG 0.500621 4.4:+19832715 None:intergenic
CAGAATATACGAGAAAAGTT+CGG 0.501978 4.4:-19833640 MS.gene35113:CDS
ATTCCTTAGCAATGAACCTA+AGG 0.505108 4.4:-19833932 MS.gene35113:CDS
TTGAAAGAATTCTTGAATGT+TGG 0.506211 4.4:+19833018 None:intergenic
ATACCAGAAACTGGTATTGA+AGG 0.507113 4.4:-19832967 MS.gene35113:CDS
ACACCTTAGGTTCATTGCTA+AGG 0.508803 4.4:+19833929 None:intergenic
TAGATGGTGCCAAACACCTT+AGG 0.511494 4.4:+19833916 None:intergenic
CTTATGTGTCAAAGTTTCCA+AGG 0.512905 4.4:-19832732 MS.gene35113:CDS
TACAATGTTCCATTTCTATG+TGG 0.513684 4.4:+19832847 None:intergenic
TTGTTTCCAACACTAGCAGC+TGG 0.514986 4.4:+19833240 None:intergenic
TGGGCTATAAGAGGTGGTGG+AGG 0.525036 4.4:-19833438 MS.gene35113:CDS
ATCCAACCAGCTGCTAGTGT+TGG 0.528310 4.4:-19833246 MS.gene35113:CDS
GTCGATTTAGTCAAACTTCG+CGG 0.531435 4.4:-19833732 MS.gene35113:CDS
TATGGATTCCAAATCATCAA+AGG 0.532792 4.4:+19832913 None:intergenic
GTTCAATCTGGTGCCACTAT+CGG 0.532890 4.4:-19833675 MS.gene35113:CDS
TTGATGAGAAAATATGGTCT+TGG 0.539868 4.4:-19833543 MS.gene35113:CDS
ATGGATTCCAAATCATCAAA+GGG 0.540969 4.4:+19832914 None:intergenic
GCTTTGGTATACTTCTTTGG+TGG 0.541590 4.4:-19833410 MS.gene35113:CDS
TCAAGGATGGTTGAGAATGA+AGG 0.541625 4.4:+19833954 None:intergenic
TTCCAACACTAGCAGCTGGT+TGG 0.544033 4.4:+19833244 None:intergenic
AGATTGAACCCAAGCAGTGT+TGG 0.553971 4.4:+19833689 None:intergenic
CATTCTCGACAGAAAAGCCA+TGG 0.561462 4.4:-19833475 MS.gene35113:CDS
CGACAGAAAAGCCATGGGAG+AGG 0.567021 4.4:-19833469 MS.gene35113:CDS
TTGGACATGTGAATCTTGGG+AGG 0.574773 4.4:+19833866 None:intergenic
GTATTGAAGGATTGTGGCAG+AGG 0.576392 4.4:-19832954 MS.gene35113:CDS
ACTTCAGGTGGTGTAACACT+TGG 0.576670 4.4:+19833057 None:intergenic
ATGTTGCAAAGCATGTAGAC+TGG 0.576679 4.4:-19832783 MS.gene35113:CDS
CATTGCTAAGGAATCAAGGA+TGG 0.582931 4.4:+19833941 None:intergenic
AGTGGTGGCCATGACTATGA+AGG 0.587071 4.4:-19833795 MS.gene35113:CDS
ACTAAATCGACAACTATGAA+AGG 0.587233 4.4:+19833744 None:intergenic
ACGAAAGTCCTTCATAGTCA+TGG 0.592842 4.4:+19833787 None:intergenic
GTAATTTGCTCCAAGAAACT+CGG 0.594947 4.4:-19833837 MS.gene35113:CDS
TCCCTGAATTGGGTTTGACA+AGG 0.596787 4.4:-19833134 MS.gene35113:CDS
TTATGTGTCAAAGTTTCCAA+GGG 0.597414 4.4:-19832731 MS.gene35113:CDS
CCTTTAAGAAGAACTTCAGG+TGG 0.600002 4.4:+19833045 None:intergenic
GTACTTGTCTATATGGCAAG+AGG 0.601770 4.4:-19832815 MS.gene35113:CDS
ACAAGCTATATAGAAGCGAC+TGG 0.606255 4.4:-19832655 MS.gene35113:CDS
CCAAAATAGATCCTCTCCCA+TGG 0.610823 4.4:+19833458 None:intergenic
ATGATTTGGAATCCATATGG+TGG 0.611221 4.4:-19832907 MS.gene35113:CDS
AAACTGGTATTGAAGGATTG+TGG 0.613890 4.4:-19832960 MS.gene35113:CDS
CTATGCACAAGCTTAGGTGT+CGG 0.620969 4.4:-19833597 MS.gene35113:CDS
TACTTGTCTATATGGCAAGA+GGG 0.628128 4.4:-19832814 MS.gene35113:CDS
TGCACAAGCTTAGGTGTCGG+TGG 0.631461 4.4:-19833594 MS.gene35113:CDS
CATCTAAGAGTAAGAAGTGG+TGG 0.632043 4.4:-19833810 MS.gene35113:CDS
TGGACATGTGAATCTTGGGA+GGG 0.643382 4.4:+19833867 None:intergenic
ATCCATCTAAGAGTAAGAAG+TGG 0.653976 4.4:-19833813 MS.gene35113:CDS
GTCACTAAAACTCTAGAACA+AGG 0.665135 4.4:-19833342 MS.gene35113:CDS
ATTCTCGACAGAAAAGCCAT+GGG 0.672433 4.4:-19833474 MS.gene35113:CDS
ATGAAATGGATATGTTCTGT+CGG 0.672862 4.4:+19834001 None:intergenic
GTCGGTGGACACATAACAGG+AGG 0.682458 4.4:-19833579 MS.gene35113:CDS
AGCTTGTGCATAGACCTGCA+GGG 0.712907 4.4:+19833607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAATAAAGATACATTT+AGG - chr4.4:19833905-19833924 MS.gene35113:CDS 15.0%
!!! TTTTTTTTTTATATTGGAAG+TGG + chr4.4:19833788-19833807 None:intergenic 15.0%
!!! AAGAGATTTTCATCAATAAA+AGG + chr4.4:19833038-19833057 None:intergenic 20.0%
! AAAAGTCAAAACACAAGAAA+AGG - chr4.4:19833808-19833827 MS.gene35113:CDS 25.0%
! AAATTCAGTACTTGTCTATA+TGG - chr4.4:19833495-19833514 MS.gene35113:CDS 25.0%
! AATGGTATCTATGAATTTGT+TGG - chr4.4:19833863-19833882 MS.gene35113:CDS 25.0%
! AATTATAGGGATCTTGATTT+GGG - chr4.4:19833620-19833639 MS.gene35113:CDS 25.0%
! CTACAAAAATTCTTCATAGA+TGG - chr4.4:19833000-19833019 MS.gene35113:CDS 25.0%
! GTGTAAATTGTTGTATGAAA+TGG + chr4.4:19832333-19832352 None:intergenic 25.0%
! TTGAAAGAATTCTTGAATGT+TGG + chr4.4:19833302-19833321 None:intergenic 25.0%
!!! TAAGGGTAATTTTAACAAGT+TGG - chr4.4:19833703-19833722 MS.gene35113:CDS 25.0%
!!! TATCTATGAATTTGTTGGAA+GGG - chr4.4:19833868-19833887 MS.gene35113:CDS 25.0%
!!! TTTTTTTATATTGGAAGTGG+TGG + chr4.4:19833785-19833804 None:intergenic 25.0%
AAAACAAGTTTCCCTGAATT+GGG - chr4.4:19833173-19833192 MS.gene35113:CDS 30.0%
AAATCTGTTCTCTATATTGC+TGG - chr4.4:19833233-19833252 MS.gene35113:CDS 30.0%
AAGATTGTCAAGAAACTAGT+TGG - chr4.4:19833207-19833226 MS.gene35113:CDS 30.0%
ACAGTAAAAACTGTTACTGT+TGG + chr4.4:19832957-19832976 None:intergenic 30.0%
ACTAAATCGACAACTATGAA+AGG + chr4.4:19832576-19832595 None:intergenic 30.0%
AGACATCCAACATTACAATT+AGG + chr4.4:19833982-19834001 None:intergenic 30.0%
ATGAAATGGATATGTTCTGT+CGG + chr4.4:19832319-19832338 None:intergenic 30.0%
ATGGATTCCAAATCATCAAA+GGG + chr4.4:19833406-19833425 None:intergenic 30.0%
CAGAATATACGAGAAAAGTT+CGG - chr4.4:19832677-19832696 MS.gene35113:CDS 30.0%
GAATTATAGGGATCTTGATT+TGG - chr4.4:19833619-19833638 MS.gene35113:CDS 30.0%
GGAACATTGATGAGAAAATA+TGG - chr4.4:19832768-19832787 MS.gene35113:CDS 30.0%
GGAAGCTTATGTGAATTATA+GGG - chr4.4:19833607-19833626 MS.gene35113:CDS 30.0%
TACAATGTTCCATTTCTATG+TGG + chr4.4:19833473-19833492 None:intergenic 30.0%
TATGGATTCCAAATCATCAA+AGG + chr4.4:19833407-19833426 None:intergenic 30.0%
TTATGTGTCAAAGTTTCCAA+GGG - chr4.4:19833586-19833605 MS.gene35113:CDS 30.0%
TTCCATTTCTATGTGGAAAA+GGG + chr4.4:19833466-19833485 None:intergenic 30.0%
TTGATGAGAAAATATGGTCT+TGG - chr4.4:19832774-19832793 MS.gene35113:CDS 30.0%
! AAGCAAAATCAGATTTTGTG+AGG - chr4.4:19833321-19833340 MS.gene35113:CDS 30.0%
! AGCAAAATCAGATTTTGTGA+GGG - chr4.4:19833322-19833341 MS.gene35113:CDS 30.0%
! TTGATGATTTGGAATCCATA+TGG - chr4.4:19833407-19833426 MS.gene35113:CDS 30.0%
! TTGGGGTTATAGGTATTTTA+AGG - chr4.4:19833685-19833704 MS.gene35113:CDS 30.0%
! TTTCCTTTAAGAAGAACTTC+AGG + chr4.4:19833278-19833297 None:intergenic 30.0%
!! ATCTATGAATTTGTTGGAAG+GGG - chr4.4:19833869-19833888 MS.gene35113:CDS 30.0%
!! GTATCTATGAATTTGTTGGA+AGG - chr4.4:19833867-19833886 MS.gene35113:CDS 30.0%
!! TGGGGTTATAGGTATTTTAA+GGG - chr4.4:19833686-19833705 MS.gene35113:CDS 30.0%
!!! TATGAAAGGTGTTTCGATTT+TGG + chr4.4:19832562-19832581 None:intergenic 30.0%
!!! TCTTACAATGCTGTTTTTCT+TGG - chr4.4:19833122-19833141 MS.gene35113:CDS 30.0%
AATCCTTCAATACCAGTTTC+TGG + chr4.4:19833356-19833375 None:intergenic 35.0%
ATCCATCTAAGAGTAAGAAG+TGG - chr4.4:19832504-19832523 MS.gene35113:intron 35.0%
ATTCCTTAGCAATGAACCTA+AGG - chr4.4:19832385-19832404 MS.gene35113:intron 35.0%
CTTATGTGTCAAAGTTTCCA+AGG - chr4.4:19833585-19833604 MS.gene35113:CDS 35.0%
CTTGGAAACTTTGACACATA+AGG + chr4.4:19833587-19833606 None:intergenic 35.0%
GAAAACAAGTTTCCCTGAAT+TGG - chr4.4:19833172-19833191 MS.gene35113:CDS 35.0%
GAGTTTCTTGGAGCAAATTA+CGG + chr4.4:19832481-19832500 None:intergenic 35.0%
GGGAAGCTTATGTGAATTAT+AGG - chr4.4:19833606-19833625 MS.gene35113:CDS 35.0%
GTAAAAACTGTTACTGTTGG+TGG + chr4.4:19832954-19832973 None:intergenic 35.0%
GTTCCATTTCTATGTGGAAA+AGG + chr4.4:19833467-19833486 None:intergenic 35.0%
GTTGAGAATGAAGGTTCATT+TGG + chr4.4:19832357-19832376 None:intergenic 35.0%
TAATTCACATAAGCTTCCCT+TGG + chr4.4:19833605-19833624 None:intergenic 35.0%
TACTTGTCTATATGGCAAGA+GGG - chr4.4:19833503-19833522 MS.gene35113:CDS 35.0%
TGAGTGCCTAATTGTAATGT+TGG - chr4.4:19833973-19833992 MS.gene35113:CDS 35.0%
TGGATCCTGAAAATGTGTTT+AGG - chr4.4:19833744-19833763 MS.gene35113:CDS 35.0%
! AAACTGGTATTGAAGGATTG+TGG - chr4.4:19833357-19833376 MS.gene35113:CDS 35.0%
! AAGATAGCCCTTTGATGATT+TGG - chr4.4:19833396-19833415 MS.gene35113:CDS 35.0%
! ATACCAGAAACTGGTATTGA+AGG - chr4.4:19833350-19833369 MS.gene35113:CDS 35.0%
! ATGATTTGGAATCCATATGG+TGG - chr4.4:19833410-19833429 MS.gene35113:CDS 35.0%
! GAAGCTTTGGTATACTTCTT+TGG - chr4.4:19832904-19832923 MS.gene35113:CDS 35.0%
! GTGAATATGAATTCGGGTTT+CGG + chr4.4:19832429-19832448 None:intergenic 35.0%
! TCATGCCTAAACACATTTTC+AGG + chr4.4:19833752-19833771 None:intergenic 35.0%
!! GATCTATTTTGGGCTATAAG+AGG - chr4.4:19832870-19832889 MS.gene35113:CDS 35.0%
!! GTAATTTGCTCCAAGAAACT+CGG - chr4.4:19832480-19832499 MS.gene35113:intron 35.0%
!! GTCACTAAAACTCTAGAACA+AGG - chr4.4:19832975-19832994 MS.gene35113:CDS 35.0%
!! TCAATACCAGTTTCTGGTAT+TGG + chr4.4:19833350-19833369 None:intergenic 35.0%
!! TCACTCATTTTTCCACCATA+TGG + chr4.4:19833425-19833444 None:intergenic 35.0%
ACAAGCTATATAGAAGCGAC+TGG - chr4.4:19833662-19833681 MS.gene35113:CDS 40.0%
ACACCTTAGGTTCATTGCTA+AGG + chr4.4:19832391-19832410 None:intergenic 40.0%
ACACTAAAACCAACACTGCT+TGG - chr4.4:19832619-19832638 MS.gene35113:intron 40.0%
ACGAAAGTCCTTCATAGTCA+TGG + chr4.4:19832533-19832552 None:intergenic 40.0%
ATGTTGCAAAGCATGTAGAC+TGG - chr4.4:19833534-19833553 MS.gene35113:CDS 40.0%
ATTCTCGACAGAAAAGCCAT+GGG - chr4.4:19832843-19832862 MS.gene35113:CDS 40.0%
CAAAGCATGTAGACTGGATT+AGG - chr4.4:19833540-19833559 MS.gene35113:CDS 40.0%
CACCACTTCTTACTCTTAGA+TGG + chr4.4:19832509-19832528 None:intergenic 40.0%
CACTAAAACCAACACTGCTT+GGG - chr4.4:19832620-19832639 MS.gene35113:intron 40.0%
CATCTAAGAGTAAGAAGTGG+TGG - chr4.4:19832507-19832526 MS.gene35113:intron 40.0%
CATTGCTAAGGAATCAAGGA+TGG + chr4.4:19832379-19832398 None:intergenic 40.0%
CCACCTGAAGTTCTTCTTAA+AGG - chr4.4:19833272-19833291 MS.gene35113:CDS 40.0%
GCTTTGGTATACTTCTTTGG+TGG - chr4.4:19832907-19832926 MS.gene35113:CDS 40.0%
GGTTCAGATAAAGACTAGAG+TGG - chr4.4:19833724-19833743 MS.gene35113:CDS 40.0%
GGTTCATTGCTAAGGAATCA+AGG + chr4.4:19832383-19832402 None:intergenic 40.0%
GTAATAGACTTCACCGATAG+TGG + chr4.4:19832658-19832677 None:intergenic 40.0%
GTACTTGTCTATATGGCAAG+AGG - chr4.4:19833502-19833521 MS.gene35113:CDS 40.0%
TACGAGAAAAGTTCGGTTCT+TGG - chr4.4:19832684-19832703 MS.gene35113:CDS 40.0%
TCAAGGATGGTTGAGAATGA+AGG + chr4.4:19832366-19832385 None:intergenic 40.0%
TCCTTGTCAAACCCAATTCA+GGG + chr4.4:19833187-19833206 None:intergenic 40.0%
TCTGTGTGTGTGTGTGATTT+AGG - chr4.4:19834001-19834020 MS.gene35113:CDS 40.0%
TGCTGCTAGTATCAGAAGAA+TGG - chr4.4:19833845-19833864 MS.gene35113:CDS 40.0%
TGCTTGGACATGTGAATCTT+GGG + chr4.4:19832457-19832476 None:intergenic 40.0%
TTCCTTGTCAAACCCAATTC+AGG + chr4.4:19833188-19833207 None:intergenic 40.0%
! AGCAATGAACCTAAGGTGTT+TGG - chr4.4:19832392-19832411 MS.gene35113:intron 40.0%
! AGCCCTTTTCCACATAGAAA+TGG - chr4.4:19833461-19833480 MS.gene35113:CDS 40.0%
! CATGGGAGAGGATCTATTTT+GGG - chr4.4:19832860-19832879 MS.gene35113:CDS 40.0%
! CCTTTAAGAAGAACTTCAGG+TGG + chr4.4:19833275-19833294 None:intergenic 40.0%
! CTATATAGAAGCGACTGGTT+GGG - chr4.4:19833667-19833686 MS.gene35113:CDS 40.0%
! GAGGGTGTGAATATGAATTC+GGG + chr4.4:19832435-19832454 None:intergenic 40.0%
! GGAGGGTGTGAATATGAATT+CGG + chr4.4:19832436-19832455 None:intergenic 40.0%
! GTCGATTTAGTCAAACTTCG+CGG - chr4.4:19832585-19832604 MS.gene35113:intron 40.0%
! TAGATGGATTCCGAGTTTCT+TGG + chr4.4:19832493-19832512 None:intergenic 40.0%
! TATATAGAAGCGACTGGTTG+GGG - chr4.4:19833668-19833687 MS.gene35113:CDS 40.0%
!! CTATTTTGGGCTATAAGAGG+TGG - chr4.4:19832873-19832892 MS.gene35113:CDS 40.0%
AACACTGCTTGGGTTCAATC+TGG - chr4.4:19832630-19832649 MS.gene35113:intron 45.0%
ACTTCAGGTGGTGTAACACT+TGG + chr4.4:19833263-19833282 None:intergenic 45.0%
AGATTGAACCCAAGCAGTGT+TGG + chr4.4:19832631-19832650 None:intergenic 45.0%
AGGGAACCAATACCAGAAAC+TGG - chr4.4:19833341-19833360 MS.gene35113:CDS 45.0%
CATTCTCGACAGAAAAGCCA+TGG - chr4.4:19832842-19832861 MS.gene35113:CDS 45.0%
CCAAAATAGATCCTCTCCCA+TGG + chr4.4:19832862-19832881 None:intergenic 45.0%
CTATGCACAAGCTTAGGTGT+CGG - chr4.4:19832720-19832739 MS.gene35113:CDS 45.0%
CTGCTTGGACATGTGAATCT+TGG + chr4.4:19832458-19832477 None:intergenic 45.0%
GCTATATAGAAGCGACTGGT+TGG - chr4.4:19833666-19833685 MS.gene35113:CDS 45.0%
GCTCGAATCGTTGATGCAAA+TGG - chr4.4:19832816-19832835 MS.gene35113:CDS 45.0%
GTATTGAAGGATTGTGGCAG+AGG - chr4.4:19833363-19833382 MS.gene35113:CDS 45.0%
TCCCTGAATTGGGTTTGACA+AGG - chr4.4:19833183-19833202 MS.gene35113:CDS 45.0%
TGGACATGTGAATCTTGGGA+GGG + chr4.4:19832453-19832472 None:intergenic 45.0%
TTGGACATGTGAATCTTGGG+AGG + chr4.4:19832454-19832473 None:intergenic 45.0%
TTGTTTCCAACACTAGCAGC+TGG + chr4.4:19833080-19833099 None:intergenic 45.0%
! AATTCGGGTTTCGGTGTAGA+TGG + chr4.4:19832420-19832439 None:intergenic 45.0%
! ACTGTTACTGTTGGTGGAAC+AGG + chr4.4:19832948-19832967 None:intergenic 45.0%
! CACATAACAGGAGGTGCATA+TGG - chr4.4:19832747-19832766 MS.gene35113:CDS 45.0%
! CCATGGGAGAGGATCTATTT+TGG - chr4.4:19832859-19832878 MS.gene35113:CDS 45.0%
! TAGATGGTGCCAAACACCTT+AGG + chr4.4:19832404-19832423 None:intergenic 45.0%
! TTTTGGGCTATAAGAGGTGG+TGG - chr4.4:19832876-19832895 MS.gene35113:CDS 45.0%
!! GTTCAATCTGGTGCCACTAT+CGG - chr4.4:19832642-19832661 MS.gene35113:CDS 45.0%
!!! ACTTTTTTTTTTTTTTATAT+TGG + chr4.4:19833794-19833813 None:intergenic 5.0%
AAGCTTGTGCATAGACCTGC+AGG + chr4.4:19832714-19832733 None:intergenic 50.0%
AGCTTGTGCATAGACCTGCA+GGG + chr4.4:19832713-19832732 None:intergenic 50.0%
AGTGGTGGCCATGACTATGA+AGG - chr4.4:19832522-19832541 MS.gene35113:intron 50.0%
ATCCAACCAGCTGCTAGTGT+TGG - chr4.4:19833071-19833090 MS.gene35113:CDS 50.0%
GCAGGTCTATGCACAAGCTT+AGG - chr4.4:19832714-19832733 MS.gene35113:CDS 50.0%
TGGAGCAAATTACGGCTGCT+TGG + chr4.4:19832473-19832492 None:intergenic 50.0%
TTCCAACACTAGCAGCTGGT+TGG + chr4.4:19833076-19833095 None:intergenic 50.0%
! AAGCGACTGGTTGGGGTTAT+AGG - chr4.4:19833675-19833694 MS.gene35113:CDS 50.0%
CGACAGAAAAGCCATGGGAG+AGG - chr4.4:19832848-19832867 MS.gene35113:CDS 55.0%
GTCGGTGGACACATAACAGG+AGG - chr4.4:19832738-19832757 MS.gene35113:CDS 55.0%
TCGGTTCTTGGTTACCCTGC+AGG - chr4.4:19832696-19832715 MS.gene35113:CDS 55.0%
! TGGGCTATAAGAGGTGGTGG+AGG - chr4.4:19832879-19832898 MS.gene35113:CDS 55.0%
!! GCTATAAGAGGTGGTGGAGG+TGG - chr4.4:19832882-19832901 MS.gene35113:CDS 55.0%
!! GGTGTCGGTGGACACATAAC+AGG - chr4.4:19832735-19832754 MS.gene35113:CDS 55.0%
!! TGCACAAGCTTAGGTGTCGG+TGG - chr4.4:19832723-19832742 MS.gene35113:CDS 55.0%
!! GGTGGTGGAGGTGGAAGCTT+TGG - chr4.4:19832891-19832910 MS.gene35113:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 19832309 19834030 19832309 ID=MS.gene35113
chr4.4 mRNA 19832309 19834030 19832309 ID=MS.gene35113.t1;Parent=MS.gene35113
chr4.4 exon 19832632 19834030 19832632 ID=MS.gene35113.t1.exon1;Parent=MS.gene35113.t1
chr4.4 CDS 19832632 19834030 19832632 ID=cds.MS.gene35113.t1;Parent=MS.gene35113.t1
chr4.4 exon 19832309 19832316 19832309 ID=MS.gene35113.t1.exon2;Parent=MS.gene35113.t1
chr4.4 CDS 19832309 19832316 19832309 ID=cds.MS.gene35113.t1;Parent=MS.gene35113.t1
Gene Sequence

>MS.gene35113

ATGAATTCCGACAGAACATATCCATTTCATACAACAATTTACACTCCAAATGAACCTTCATTCTCAACCATCCTTGATTCCTTAGCAATGAACCTAAGGTGTTTGGCACCATCTACACCGAAACCCGAATTCATATTCACACCCTCCCAAGATTCACATGTCCAAGCAGCCGTAATTTGCTCCAAGAAACTCGGAATCCATCTAAGAGTAAGAAGTGGTGGCCATGACTATGAAGGACTTTCGTATGTTTCCAAAATCGAAACACCTTTCATAGTTGTCGATTTAGTCAAACTTCGCGGCATAAGCATCGACACTAAAACCAACACTGCTTGGGTTCAATCTGGTGCCACTATCGGTGAAGTCTATTACAGAATATACGAGAAAAGTTCGGTTCTTGGTTACCCTGCAGGTCTATGCACAAGCTTAGGTGTCGGTGGACACATAACAGGAGGTGCATATGGAACATTGATGAGAAAATATGGTCTTGGTGTTGATAATGTCTTAGATGCTCGAATCGTTGATGCAAATGGAAACATTCTCGACAGAAAAGCCATGGGAGAGGATCTATTTTGGGCTATAAGAGGTGGTGGAGGTGGAAGCTTTGGTATACTTCTTTGGTGGAAGATAAAGCTTGTTCCTGTTCCACCAACAGTAACAGTTTTTACTGTCACTAAAACTCTAGAACAAGGTGCTACAAAAATTCTTCATAGATGGCAAGAAGTAGCACCTTTTATTGATGAAAATCTCTTTATTAGAGTCATCATCCAACCAGCTGCTAGTGTTGGAAACAAAACTCAAAGAACTATTACAACTTCTTACAATGCTGTTTTTCTTGGTGAAGCAAGAACACTACTTCAAGTCATGAAAACAAGTTTCCCTGAATTGGGTTTGACAAGGAAAGATTGTCAAGAAACTAGTTGGATCAAATCTGTTCTCTATATTGCTGGATATCCAAGTGTTACACCACCTGAAGTTCTTCTTAAAGGAAAACCAACATTCAAGAATTCTTTCAAAGCAAAATCAGATTTTGTGAGGGAACCAATACCAGAAACTGGTATTGAAGGATTGTGGCAGAGGCTGTTGAGTGAAGATAGCCCTTTGATGATTTGGAATCCATATGGTGGAAAAATGAGTGAATTTTCAGAATCAGATAGCCCTTTTCCACATAGAAATGGAACATTGTATAAAATTCAGTACTTGTCTATATGGCAAGAGGGTGACAAGAATGTTGCAAAGCATGTAGACTGGATTAGGAAGCTCTATAACTATATGACTCCTTATGTGTCAAAGTTTCCAAGGGAAGCTTATGTGAATTATAGGGATCTTGATTTGGGAATTAACTCTAAGAATAGTACAAGCTATATAGAAGCGACTGGTTGGGGTTATAGGTATTTTAAGGGTAATTTTAACAAGTTGGTTCAGATAAAGACTAGAGTGGATCCTGAAAATGTGTTTAGGCATGAACAAAGTATTCCACCACTTCCAATATAAAAAAAAAAAAAAGTCAAAACACAAGAAAAGGAAAATAAGACACTTTGCTGCTAGTATCAGAAGAATGGTATCTATGAATTTGTTGGAAGGGGTCATATCTAGTTTAGAAAAATAAAGATACATTTAGGTTTGTTACTTTTATTGTATTATTAATGTTGTAAGATACTACTGATTGAGTGCCTAATTGTAATGTTGGATGTCTCTGTGTGTGTGTGTGATTTAGGTGTCTAA
Protein sequence

>MS.gene35113.t1

MNSDRTYPFHTTIYTPNEPSFSTILDSLAMNLRCLAPSTPKPEFIFTPSQDSHVQAAVICSKKLGIHLRVRSGGHDYEGLSYVSKIETPFIVVDLVKLRGISIDTKTNTAWVQSGATIGEVYYRIYEKSSVLGYPAGLCTSLGVGGHITGGAYGTLMRKYGLGVDNVLDARIVDANGNILDRKAMGEDLFWAIRGGGGGSFGILLWWKIKLVPVPPTVTVFTVTKTLEQGATKILHRWQEVAPFIDENLFIRVIIQPAASVGNKTQRTITTSYNAVFLGEARTLLQVMKTSFPELGLTRKDCQETSWIKSVLYIAGYPSVTPPEVLLKGKPTFKNSFKAKSDFVREPIPETGIEGLWQRLLSEDSPLMIWNPYGGKMSEFSESDSPFPHRNGTLYKIQYLSIWQEGDKNVAKHVDWIRKLYNYMTPYVSKFPREAYVNYRDLDLGINSKNSTSYIEATGWGYRYFKGV