AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36421

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36421.t1 MTR_4g094418 95.690 464 20 0 1 464 1 464 0.0 919
MS.gene36421.t1 MTR_4g094488 73.455 437 109 3 1 434 104 536 0.0 645
MS.gene36421.t1 MTR_4g094478 71.789 436 118 2 1 435 104 535 0.0 638
MS.gene36421.t1 MTR_4g094495 70.481 437 122 2 1 434 107 539 0.0 627
MS.gene36421.t1 MTR_4g094492 69.300 443 128 3 1 440 104 541 0.0 622
MS.gene36421.t1 MTR_4g094480 70.345 435 124 3 1 434 104 534 0.0 620
MS.gene36421.t1 MTR_3g009820 58.716 436 170 4 1 436 98 523 0.0 524
MS.gene36421.t1 MTR_3g009760 58.486 436 171 4 1 436 98 523 0.0 522
MS.gene36421.t1 MTR_3g009880 58.202 445 173 5 1 445 96 527 0.0 517
MS.gene36421.t1 MTR_2g031560 55.530 443 190 3 1 443 96 531 1.60e-179 514
MS.gene36421.t1 MTR_2g031390 55.149 437 186 5 1 435 101 529 2.02e-178 511
MS.gene36421.t1 MTR_2g031590 57.207 444 179 5 1 440 101 537 4.40e-176 504
MS.gene36421.t1 MTR_2g031460 58.072 446 176 5 1 446 104 538 2.73e-173 498
MS.gene36421.t1 MTR_4g094498 59.174 436 168 4 1 436 110 535 1.14e-171 494
MS.gene36421.t1 MTR_2g031420 57.339 436 175 5 1 436 104 528 1.69e-170 490
MS.gene36421.t1 MTR_4g094520 54.462 437 188 6 1 435 105 532 2.37e-163 472
MS.gene36421.t1 MTR_4g094518 55.378 437 185 5 1 435 102 530 9.85e-163 470
MS.gene36421.t1 MTR_2g031490 52.184 435 204 3 1 435 108 538 6.46e-156 453
MS.gene36421.t1 MTR_2g031510 51.945 437 206 3 1 437 110 542 3.98e-154 449
MS.gene36421.t1 MTR_4g094515 53.793 435 194 3 1 435 723 1150 5.82e-154 467
MS.gene36421.t1 MTR_4g094515 51.030 437 203 4 1 434 111 539 5.78e-143 438
MS.gene36421.t1 MTR_4g088355 52.847 439 195 5 1 436 105 534 6.52e-150 438
MS.gene36421.t1 MTR_4g091190 51.034 435 203 4 1 434 102 527 1.20e-148 434
MS.gene36421.t1 MTR_4g091200 51.259 437 205 4 1 436 98 527 1.33e-148 434
MS.gene36421.t1 MTR_4g091150 51.487 437 204 5 1 435 110 540 2.48e-148 434
MS.gene36421.t1 MTR_4g091170 49.078 434 197 4 1 434 132 541 5.63e-148 434
MS.gene36421.t1 MTR_4g057990 51.030 437 203 4 1 436 97 523 9.20e-147 429
MS.gene36421.t1 MTR_6g017205 51.494 435 203 4 1 434 98 525 2.42e-146 429
MS.gene36421.t1 MTR_2g023590 48.975 439 214 4 1 438 40 469 4.25e-140 410
MS.gene36421.t1 MTR_1g076830 45.909 440 226 7 1 436 105 536 4.44e-121 364
MS.gene36421.t1 MTR_3g009850 57.447 235 94 3 202 436 1 229 6.57e-90 273
MS.gene36421.t1 MTR_2g031600 70.629 143 42 0 1 143 102 244 2.16e-61 206
MS.gene36421.t1 MTR_2g031600 53.801 171 75 3 271 440 238 405 3.15e-55 189
MS.gene36421.t1 MTR_2g084370 47.748 111 36 2 25 135 1 89 4.05e-30 113
MS.gene36421.t1 MTR_2g084405 47.917 96 44 2 25 120 1 90 1.13e-19 84.3
MS.gene36421.t1 MTR_8g062280 35.354 99 34 3 334 432 4 72 3.74e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36421.t1 AT4G20820 54.128 436 184 5 1 436 108 527 2.56e-165 477
MS.gene36421.t1 AT1G30760 53.899 436 187 5 1 434 126 549 6.76e-164 474
MS.gene36421.t1 AT1G30760 53.899 436 187 5 1 434 108 531 7.33e-164 473
MS.gene36421.t1 AT5G44360 53.881 438 184 7 1 435 108 530 8.62e-164 473
MS.gene36421.t1 AT5G44360 53.881 438 184 7 1 435 124 546 9.77e-164 473
MS.gene36421.t1 AT2G34790 54.442 439 180 8 1 434 106 529 1.92e-162 469
MS.gene36421.t1 AT5G44440 52.834 441 193 5 1 437 102 531 2.96e-162 469
MS.gene36421.t1 AT5G44440 52.834 441 193 5 1 437 172 601 2.03e-161 469
MS.gene36421.t1 AT4G20860 52.523 436 195 5 1 435 105 529 9.43e-161 465
MS.gene36421.t1 AT5G44400 52.392 439 195 7 1 435 108 536 7.65e-159 461
MS.gene36421.t1 AT5G44390 51.708 439 200 7 1 435 110 540 3.55e-158 459
MS.gene36421.t1 AT5G44380 53.986 439 188 7 1 435 111 539 1.82e-154 449
MS.gene36421.t1 AT5G44380 53.986 439 188 7 1 435 151 579 4.04e-154 450
MS.gene36421.t1 AT5G44410 51.794 446 194 6 1 441 106 535 4.42e-152 443
MS.gene36421.t1 AT1G11770 52.414 435 198 6 1 435 104 529 9.89e-151 440
MS.gene36421.t1 AT1G30700 53.653 438 190 6 1 435 99 526 1.39e-150 439
MS.gene36421.t1 AT1G26420 52.511 438 192 9 1 435 101 525 9.30e-148 432
MS.gene36421.t1 AT4G20840 51.270 433 202 5 3 435 112 535 7.78e-147 430
MS.gene36421.t1 AT1G26380 52.184 435 198 6 1 435 101 525 1.55e-145 427
MS.gene36421.t1 AT1G30710 49.773 440 202 6 1 436 105 529 2.67e-145 426
MS.gene36421.t1 AT1G26390 50.805 435 206 4 1 435 101 527 2.94e-145 426
MS.gene36421.t1 AT1G26410 49.655 435 209 6 1 435 125 549 1.13e-143 422
MS.gene36421.t1 AT1G26400 50.926 432 201 6 1 432 101 521 9.92e-143 419
MS.gene36421.t1 AT1G34575 50.459 436 198 6 1 433 102 522 1.57e-142 419
MS.gene36421.t1 AT1G01980 52.392 439 195 8 2 435 105 534 1.58e-142 419
MS.gene36421.t1 AT1G30730 49.770 434 207 5 1 433 100 523 1.51e-139 411
MS.gene36421.t1 AT4G20830 50.808 433 204 5 3 435 113 536 2.71e-137 406
MS.gene36421.t1 AT4G20800 48.741 437 213 6 1 436 100 526 2.93e-137 405
MS.gene36421.t1 AT1G30740 50.343 437 206 7 1 435 104 531 6.08e-137 405
MS.gene36421.t1 AT1G30740 50.343 437 206 7 1 435 101 528 6.58e-137 404
MS.gene36421.t1 AT4G20830 49.673 459 208 7 3 461 113 548 9.95e-137 405
MS.gene36421.t1 AT1G30720 49.539 434 208 5 1 433 101 524 3.25e-135 400
MS.gene36421.t1 AT2G34810 45.330 439 232 4 1 436 107 540 1.26e-130 389

Find 106 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAACATTAGGGTCATTT+TGG 0.158436 4.1:-19082994 MS.gene36421:CDS
GCTATAAGCAAGCTAGTATT+TGG 0.193317 4.1:-19082356 MS.gene36421:CDS
AGAGAGCCTATTCCTGATAT+TGG 0.262653 4.1:-19082693 MS.gene36421:CDS
TGACAATATTCAGAGTTTCT+AGG 0.284906 4.1:-19083085 MS.gene36421:CDS
CTGAATATTGTCACAGTTGA+TGG 0.291976 4.1:+19083095 None:intergenic
ACATTTGAATCCTTGTTTCT+TGG 0.300277 4.1:-19082924 MS.gene36421:CDS
ATTCCTGATATTGGGTTAGA+AGG 0.305269 4.1:-19082684 MS.gene36421:CDS
TTGTTTCTTGGAGGTGTAGA+TGG 0.315456 4.1:-19082912 MS.gene36421:CDS
AAGCAAAACTCTTGGCTTCC+CGG 0.319839 4.1:-19083354 MS.gene36421:CDS
CAATGGGTGAGGATCTGTTT+TGG 0.324567 4.1:-19083187 MS.gene36421:CDS
TGGAATAATGGATGAGATTT+CGG 0.325191 4.1:-19082598 MS.gene36421:CDS
ATACCTTCTCTGTCTCGTTT+TGG 0.346393 4.1:-19082243 MS.gene36421:CDS
CACTCCTTATGGTGGAATAA+TGG 0.346906 4.1:-19082610 MS.gene36421:CDS
GATGCGTAAGTTTGGTCTTT+CGG 0.350217 4.1:-19083270 MS.gene36421:CDS
TAAGAATAACTTCAAAAGAT+TGG 0.350745 4.1:-19082319 MS.gene36421:CDS
AACCCTTCTAACCCAATATC+AGG 0.364075 4.1:+19082681 None:intergenic
TTGATGAGGACTTAAACATT+AGG 0.365884 4.1:-19083004 MS.gene36421:CDS
CTATAAGCAAGCTAGTATTT+GGG 0.368691 4.1:-19082355 MS.gene36421:CDS
CTTATCAGTGGCGGCGGCTA+TGG 0.371457 4.1:-19083299 MS.gene36421:CDS
GAGAGCCTATTCCTGATATT+GGG 0.380379 4.1:-19082692 MS.gene36421:CDS
GGAGGTGGAGGTGCAAGCTT+TGG 0.389215 4.1:-19083155 MS.gene36421:CDS
TTTGAATCCTTGTTTCTTGG+AGG 0.404634 4.1:-19082921 MS.gene36421:CDS
ATATTGGGTTAGAAGGGTTA+TGG 0.406546 4.1:-19082677 MS.gene36421:CDS
CATCTACTTGGACTTCTTGA+AGG 0.407407 4.1:+19083447 None:intergenic
CTTGGAGACTTTGACACAAA+AGG 0.412025 4.1:+19082441 None:intergenic
TGATGAGGACTTAAACATTA+GGG 0.412260 4.1:-19083003 MS.gene36421:CDS
ATGAGATCAATGACAACAAA+TGG 0.413992 4.1:+19083473 None:intergenic
GTGTTGATATTCACTCCTTA+TGG 0.417599 4.1:-19082621 MS.gene36421:CDS
TCTAACCCAATATCAGGAAT+AGG 0.418514 4.1:+19082687 None:intergenic
GGATTCTTGATGCGTAAGTT+TGG 0.420162 4.1:-19083278 MS.gene36421:CDS
TTCTATTCAACAAAACTTCA+AGG 0.420531 4.1:+19082760 None:intergenic
AATTATAGAGATCTTGATAT+TGG 0.441086 4.1:-19082405 MS.gene36421:CDS
AAGATTGTACTGAGATGAGT+TGG 0.447941 4.1:-19082839 MS.gene36421:CDS
GTTCAAGGAGGGGCAACTGC+TGG 0.456565 4.1:-19083404 MS.gene36421:CDS
GGTTCGTGACAGAGAATCAA+TGG 0.464766 4.1:-19083204 MS.gene36421:CDS
TGCCCTACTGTAGGTGTTAG+CGG 0.465359 4.1:-19083323 MS.gene36421:CDS
AATAGCAGTGCATGGGTTCA+AGG 0.466266 4.1:-19083419 MS.gene36421:CDS
TAATTCACATATGCACCTCT+TGG 0.471919 4.1:+19082423 None:intergenic
TCTCTAACCAAACCAAGCTC+TGG 0.473432 4.1:+19082861 None:intergenic
GGAGTTTCCAGAGCTTGGTT+TGG 0.476037 4.1:-19082868 MS.gene36421:CDS
TAGAAAACTTTATAGTTACA+TGG 0.480388 4.1:-19082466 MS.gene36421:CDS
TGGAAACTCCTCTTCCATCA+AGG 0.482075 4.1:+19082881 None:intergenic
GAAGAGGAGTTTCCAGAGCT+TGG 0.483846 4.1:-19082873 MS.gene36421:CDS
ATTCAGAGTTTCTAGGACAC+TGG 0.484970 4.1:-19083078 MS.gene36421:CDS
AGATGGACTTATCCCCTTGA+TGG 0.488351 4.1:-19082895 MS.gene36421:CDS
TCATCCATTATTCCACCATA+AGG 0.498834 4.1:+19082606 None:intergenic
TGGGCTATTAGAGGAGGTGG+AGG 0.498949 4.1:-19083167 MS.gene36421:CDS
TTCCTGATATTGGGTTAGAA+GGG 0.500382 4.1:-19082683 MS.gene36421:CDS
TTGGAAAGAGGAAGGTGATG+AGG 0.502301 4.1:-19082514 MS.gene36421:CDS
ACATAATTGATGTGAATGGT+AGG 0.505623 4.1:-19083226 MS.gene36421:CDS
AAAACTCTTGGCTTCCCGGC+AGG 0.513479 4.1:-19083350 MS.gene36421:CDS
ATTCCATTCCCACACAGAGC+TGG 0.515576 4.1:-19082567 MS.gene36421:CDS
AATACATTGCCTTGTGAGGA+AGG 0.517365 4.1:-19082201 MS.gene36421:CDS
TCTATTCAACAAAACTTCAA+GGG 0.517551 4.1:+19082761 None:intergenic
TTTCCAGCTCTGTGTGGGAA+TGG 0.526072 4.1:+19082564 None:intergenic
AAATGGAACTTCAGAAACAT+AGG 0.532136 4.1:+19083490 None:intergenic
CTGCCACTTGTGAATAAGCC+TGG 0.540705 4.1:+19083043 None:intergenic
GGAAACTCCTCTTCCATCAA+GGG 0.545602 4.1:+19082882 None:intergenic
CGTAGTCGTGGCCACCACTT+CGG 0.548447 4.1:+19083522 None:intergenic
ATGTAGAAAATAGCAGTGCA+TGG 0.560180 4.1:-19083427 MS.gene36421:CDS
CATAATTGATGTGAATGGTA+GGG 0.562579 4.1:-19083225 MS.gene36421:CDS
TTGATATTCACTCCTTATGG+TGG 0.565905 4.1:-19082618 MS.gene36421:CDS
ATGCAATTATTAGAGTTCCC+TGG 0.575808 4.1:-19082177 MS.gene36421:CDS
AATTGCATCCCTTCCTCACA+AGG 0.581641 4.1:+19082192 None:intergenic
GCTTTGGAGTTATCATAGCA+TGG 0.581899 4.1:-19083139 MS.gene36421:CDS
GCAGTGCATGGGTTCAAGGA+GGG 0.582300 4.1:-19083415 MS.gene36421:CDS
ACTTATCCCCTTGATGGAAG+AGG 0.583355 4.1:-19082889 MS.gene36421:CDS
AGTCCGCTAACACCTACAGT+AGG 0.585729 4.1:+19083320 None:intergenic
ACAGTAGGGCAAATACCTGC+CGG 0.587491 4.1:+19083335 None:intergenic
TTGTTGAATAGAACTCATAG+TGG 0.587672 4.1:-19082750 MS.gene36421:CDS
TGACAGAGAATCAATGGGTG+AGG 0.587800 4.1:-19083198 MS.gene36421:CDS
CTATTCAACAAAACTTCAAG+GGG 0.590722 4.1:+19082762 None:intergenic
AGCGGACTTATCAGTGGCGG+CGG 0.591156 4.1:-19083305 MS.gene36421:CDS
TGTAGAAAATAGCAGTGCAT+GGG 0.602666 4.1:-19083426 MS.gene36421:CDS
CAGTAGGGCAAATACCTGCC+GGG 0.605644 4.1:+19083336 None:intergenic
ATTGGTGTGAATAATAACAA+TGG 0.605661 4.1:-19082387 MS.gene36421:CDS
GTTCGTGACAGAGAATCAAT+GGG 0.608043 4.1:-19083203 MS.gene36421:CDS
AGGACAGACCTTCGTAGTCG+TGG 0.610475 4.1:+19083510 None:intergenic
GCTCACATAATTGATGTGAA+TGG 0.610798 4.1:-19083230 MS.gene36421:CDS
CAGTGCATGGGTTCAAGGAG+GGG 0.614481 4.1:-19083414 MS.gene36421:CDS
CACTGGAACAAAATGCAACC+AGG 0.614754 4.1:-19083061 MS.gene36421:CDS
GCAGGTATTTGCCCTACTGT+AGG 0.615009 4.1:-19083332 MS.gene36421:CDS
ATCTACACCTCCAAGAAACA+AGG 0.617374 4.1:+19082914 None:intergenic
GCATAATACATTGCCTTGTG+AGG 0.617909 4.1:-19082205 MS.gene36421:CDS
ATTGAATCAGTTCTCTACCA+TGG 0.621990 4.1:-19082816 MS.gene36421:CDS
AAGAAACAAGGATTCAAATG+TGG 0.623440 4.1:+19082926 None:intergenic
ATACATTGCCTTGTGAGGAA+GGG 0.623495 4.1:-19082200 MS.gene36421:CDS
TATAAGCAAGCTAGTATTTG+GGG 0.624423 4.1:-19082354 MS.gene36421:CDS
GTCCGCTAACACCTACAGTA+GGG 0.627164 4.1:+19083321 None:intergenic
CAGATTCGAATCCGAAGTGG+TGG 0.628502 4.1:-19083533 MS.gene36421:CDS
CAACCAGGCTTATTCACAAG+TGG 0.642716 4.1:-19083046 MS.gene36421:CDS
AAATGTTTCCAGCTCTGTGT+GGG 0.643042 4.1:+19082559 None:intergenic
GGTGTTAGCGGACTTATCAG+TGG 0.652652 4.1:-19083311 MS.gene36421:CDS
GAATCAGTTCTCTACCATGG+TGG 0.653386 4.1:-19082813 MS.gene36421:CDS
AGTAGCAAATAAGCTTGATG+AGG 0.653614 4.1:-19083018 MS.gene36421:CDS
TTACCAAAACGAGACAGAGA+AGG 0.660634 4.1:+19082240 None:intergenic
AGCAGTGCATGGGTTCAAGG+AGG 0.665984 4.1:-19083416 MS.gene36421:CDS
GAAACTCCTCTTCCATCAAG+GGG 0.668982 4.1:+19082883 None:intergenic
ATGCAGATTCGAATCCGAAG+TGG 0.674707 4.1:-19083536 MS.gene36421:CDS
TTTGTGTCAAAGTCTCCAAG+AGG 0.676435 4.1:-19082438 MS.gene36421:CDS
TAAATGTTTCCAGCTCTGTG+TGG 0.685122 4.1:+19082558 None:intergenic
TGAAAACGAGGCTAAATCTG+CGG 0.694314 4.1:-19082643 MS.gene36421:CDS
AGTGGTGGCCACGACTACGA+AGG 0.696564 4.1:-19083518 MS.gene36421:CDS
GTTAGCGGACTTATCAGTGG+CGG 0.699597 4.1:-19083308 MS.gene36421:CDS
TGAATACTAGTACTCAAACA+AGG 0.702644 4.1:-19082965 MS.gene36421:CDS
AATCACTTGTAAAACCACCA+TGG 0.721311 4.1:+19082799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAAAAAAGGTATATAAAT+TGG - chr4.1:19083203-19083222 MS.gene36421:CDS 15.0%
!! AATTATAGAGATCTTGATAT+TGG - chr4.1:19083289-19083308 MS.gene36421:CDS 20.0%
!! TAAGAATAACTTCAAAAGAT+TGG - chr4.1:19083375-19083394 MS.gene36421:CDS 20.0%
!! TAGAAAACTTTATAGTTACA+TGG - chr4.1:19083228-19083247 MS.gene36421:CDS 20.0%
! ATTGGTGTGAATAATAACAA+TGG - chr4.1:19083307-19083326 MS.gene36421:CDS 25.0%
! TCTATTCAACAAAACTTCAA+GGG + chr4.1:19082936-19082955 None:intergenic 25.0%
! TTCTATTCAACAAAACTTCA+AGG + chr4.1:19082937-19082956 None:intergenic 25.0%
!! TCATTTCTGAAAAAGTTTAG+AGG + chr4.1:19083424-19083443 None:intergenic 25.0%
!!! AAAAAACTAAGTGCTGAATT+TGG + chr4.1:19083162-19083181 None:intergenic 25.0%
!!! AAATTCAGCACTTAGTTTTT+TGG - chr4.1:19083161-19083180 MS.gene36421:CDS 25.0%
!!! GTTTTGGTAACATAACAATA+AGG - chr4.1:19083467-19083486 MS.gene36421:CDS 25.0%
AAATGGAACTTCAGAAACAT+AGG + chr4.1:19082207-19082226 None:intergenic 30.0%
AAGAAACAAGGATTCAAATG+TGG + chr4.1:19082771-19082790 None:intergenic 30.0%
ACATAATTGATGTGAATGGT+AGG - chr4.1:19082468-19082487 MS.gene36421:CDS 30.0%
ATGAGATCAATGACAACAAA+TGG + chr4.1:19082224-19082243 None:intergenic 30.0%
CATAATTGATGTGAATGGTA+GGG - chr4.1:19082469-19082488 MS.gene36421:CDS 30.0%
CTATAAGCAAGCTAGTATTT+GGG - chr4.1:19083339-19083358 MS.gene36421:CDS 30.0%
CTATTCAACAAAACTTCAAG+GGG + chr4.1:19082935-19082954 None:intergenic 30.0%
TATAAGCAAGCTAGTATTTG+GGG - chr4.1:19083340-19083359 MS.gene36421:CDS 30.0%
TGAATACTAGTACTCAAACA+AGG - chr4.1:19082729-19082748 MS.gene36421:CDS 30.0%
TGACAATATTCAGAGTTTCT+AGG - chr4.1:19082609-19082628 MS.gene36421:CDS 30.0%
TGATGAGGACTTAAACATTA+GGG - chr4.1:19082691-19082710 MS.gene36421:CDS 30.0%
TTGATGAGGACTTAAACATT+AGG - chr4.1:19082690-19082709 MS.gene36421:CDS 30.0%
TTGTTGAATAGAACTCATAG+TGG - chr4.1:19082944-19082963 MS.gene36421:CDS 30.0%
! ACATTTGAATCCTTGTTTCT+TGG - chr4.1:19082770-19082789 MS.gene36421:CDS 30.0%
! CTTAAACATTAGGGTCATTT+TGG - chr4.1:19082700-19082719 MS.gene36421:CDS 30.0%
!! ATTTTATATGTAGAGCACCA+GGG + chr4.1:19083537-19083556 None:intergenic 30.0%
!! TATTTTATATGTAGAGCACC+AGG + chr4.1:19083538-19083557 None:intergenic 30.0%
!! TGGAATAATGGATGAGATTT+CGG - chr4.1:19083096-19083115 MS.gene36421:CDS 30.0%
!!! TGCTATTTTCTACATCTACT+TGG + chr4.1:19082262-19082281 None:intergenic 30.0%
!!! TTAGTTTTTTGGAAAGAGGA+AGG - chr4.1:19083172-19083191 MS.gene36421:CDS 30.0%
AAGATTGTACTGAGATGAGT+TGG - chr4.1:19082855-19082874 MS.gene36421:CDS 35.0%
AAGGTGATGAGGTAGAAAAA+AGG - chr4.1:19083191-19083210 MS.gene36421:CDS 35.0%
AATCACTTGTAAAACCACCA+TGG + chr4.1:19082898-19082917 None:intergenic 35.0%
AGCCAAAAAAGCAAAACTCT+TGG - chr4.1:19082332-19082351 MS.gene36421:CDS 35.0%
AGTAGCAAATAAGCTTGATG+AGG - chr4.1:19082676-19082695 MS.gene36421:CDS 35.0%
ATGCAATTATTAGAGTTCCC+TGG - chr4.1:19083517-19083536 MS.gene36421:CDS 35.0%
ATGTAGAAAATAGCAGTGCA+TGG - chr4.1:19082267-19082286 MS.gene36421:CDS 35.0%
ATTGAATCAGTTCTCTACCA+TGG - chr4.1:19082878-19082897 MS.gene36421:CDS 35.0%
CTGAATATTGTCACAGTTGA+TGG + chr4.1:19082602-19082621 None:intergenic 35.0%
GCTATAAGCAAGCTAGTATT+TGG - chr4.1:19083338-19083357 MS.gene36421:CDS 35.0%
GCTCACATAATTGATGTGAA+TGG - chr4.1:19082464-19082483 MS.gene36421:CDS 35.0%
TAATTCACATATGCACCTCT+TGG + chr4.1:19083274-19083293 None:intergenic 35.0%
TCATCCATTATTCCACCATA+AGG + chr4.1:19083091-19083110 None:intergenic 35.0%
TCTAACCCAATATCAGGAAT+AGG + chr4.1:19083010-19083029 None:intergenic 35.0%
TGTAGAAAATAGCAGTGCAT+GGG - chr4.1:19082268-19082287 MS.gene36421:CDS 35.0%
TTGATATTCACTCCTTATGG+TGG - chr4.1:19083076-19083095 MS.gene36421:CDS 35.0%
! ATATTGGGTTAGAAGGGTTA+TGG - chr4.1:19083017-19083036 MS.gene36421:CDS 35.0%
! ATTCCTGATATTGGGTTAGA+AGG - chr4.1:19083010-19083029 MS.gene36421:CDS 35.0%
! GCCAATGTTTTATGAAAACG+AGG - chr4.1:19083039-19083058 MS.gene36421:CDS 35.0%
! GCCTCGTTTTCATAAAACAT+TGG + chr4.1:19083043-19083062 None:intergenic 35.0%
! GTGTTGATATTCACTCCTTA+TGG - chr4.1:19083073-19083092 MS.gene36421:CDS 35.0%
! TTCCTGATATTGGGTTAGAA+GGG - chr4.1:19083011-19083030 MS.gene36421:CDS 35.0%
! TTTGAATCCTTGTTTCTTGG+AGG - chr4.1:19082773-19082792 MS.gene36421:CDS 35.0%
!!! AGCCAAGAGTTTTGCTTTTT+TGG + chr4.1:19082337-19082356 None:intergenic 35.0%
!!! GCACTTAGTTTTTTGGAAAG+AGG - chr4.1:19083168-19083187 MS.gene36421:CDS 35.0%
!!! TAGTGGTGTGTTGTTTTTCA+AGG - chr4.1:19082961-19082980 MS.gene36421:CDS 35.0%
AAATGTTTCCAGCTCTGTGT+GGG + chr4.1:19083138-19083157 None:intergenic 40.0%
AACCCTTCTAACCCAATATC+AGG + chr4.1:19083016-19083035 None:intergenic 40.0%
AATACATTGCCTTGTGAGGA+AGG - chr4.1:19083493-19083512 MS.gene36421:CDS 40.0%
AGAGAGCCTATTCCTGATAT+TGG - chr4.1:19083001-19083020 MS.gene36421:CDS 40.0%
ATACATTGCCTTGTGAGGAA+GGG - chr4.1:19083494-19083513 MS.gene36421:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG + chr4.1:19082783-19082802 None:intergenic 40.0%
CATCTACTTGGACTTCTTGA+AGG + chr4.1:19082250-19082269 None:intergenic 40.0%
CTTGGAGACTTTGACACAAA+AGG + chr4.1:19083256-19083275 None:intergenic 40.0%
GAGAGCCTATTCCTGATATT+GGG - chr4.1:19083002-19083021 MS.gene36421:CDS 40.0%
GCATAATACATTGCCTTGTG+AGG - chr4.1:19083489-19083508 MS.gene36421:CDS 40.0%
GGATTCTTGATGCGTAAGTT+TGG - chr4.1:19082416-19082435 MS.gene36421:CDS 40.0%
GTTCGTGACAGAGAATCAAT+GGG - chr4.1:19082491-19082510 MS.gene36421:CDS 40.0%
TAAATGTTTCCAGCTCTGTG+TGG + chr4.1:19083139-19083158 None:intergenic 40.0%
TGAAAACGAGGCTAAATCTG+CGG - chr4.1:19083051-19083070 MS.gene36421:CDS 40.0%
TTACCAAAACGAGACAGAGA+AGG + chr4.1:19083457-19083476 None:intergenic 40.0%
TTTGTGTCAAAGTCTCCAAG+AGG - chr4.1:19083256-19083275 MS.gene36421:CDS 40.0%
! AATGGGTGAGGATCTGTTTT+GGG - chr4.1:19082508-19082527 MS.gene36421:CDS 40.0%
! ATACCTTCTCTGTCTCGTTT+TGG - chr4.1:19083451-19083470 MS.gene36421:CDS 40.0%
! CACTCCTTATGGTGGAATAA+TGG - chr4.1:19083084-19083103 MS.gene36421:CDS 40.0%
! GATGCGTAAGTTTGGTCTTT+CGG - chr4.1:19082424-19082443 MS.gene36421:CDS 40.0%
! TTGTTTCTTGGAGGTGTAGA+TGG - chr4.1:19082782-19082801 MS.gene36421:CDS 40.0%
!! ACATTAGGGTCATTTTGGAG+AGG - chr4.1:19082705-19082724 MS.gene36421:CDS 40.0%
!! ATTCAGAGTTTCTAGGACAC+TGG - chr4.1:19082616-19082635 MS.gene36421:CDS 40.0%
!! CATTAGGGTCATTTTGGAGA+GGG - chr4.1:19082706-19082725 MS.gene36421:CDS 40.0%
!! GATCTGTTTTGGGCTATTAG+AGG - chr4.1:19082518-19082537 MS.gene36421:CDS 40.0%
!! GCTTTGGAGTTATCATAGCA+TGG - chr4.1:19082555-19082574 MS.gene36421:CDS 40.0%
AAGCAAAACTCTTGGCTTCC+CGG - chr4.1:19082340-19082359 MS.gene36421:CDS 45.0%
AATAGCAGTGCATGGGTTCA+AGG - chr4.1:19082275-19082294 MS.gene36421:CDS 45.0%
AATTGCATCCCTTCCTCACA+AGG + chr4.1:19083505-19083524 None:intergenic 45.0%
ACTTATCCCCTTGATGGAAG+AGG - chr4.1:19082805-19082824 MS.gene36421:CDS 45.0%
ATGCAGATTCGAATCCGAAG+TGG - chr4.1:19082158-19082177 MS.gene36421:CDS 45.0%
CAACCAGGCTTATTCACAAG+TGG - chr4.1:19082648-19082667 MS.gene36421:CDS 45.0%
CACTGGAACAAAATGCAACC+AGG - chr4.1:19082633-19082652 MS.gene36421:CDS 45.0%
GAAACTCCTCTTCCATCAAG+GGG + chr4.1:19082814-19082833 None:intergenic 45.0%
GAATCAGTTCTCTACCATGG+TGG - chr4.1:19082881-19082900 MS.gene36421:CDS 45.0%
GGAAACTCCTCTTCCATCAA+GGG + chr4.1:19082815-19082834 None:intergenic 45.0%
GGTTCGTGACAGAGAATCAA+TGG - chr4.1:19082490-19082509 MS.gene36421:CDS 45.0%
TCTCTAACCAAACCAAGCTC+TGG + chr4.1:19082836-19082855 None:intergenic 45.0%
TGACAGAGAATCAATGGGTG+AGG - chr4.1:19082496-19082515 MS.gene36421:CDS 45.0%
TGGAAACTCCTCTTCCATCA+AGG + chr4.1:19082816-19082835 None:intergenic 45.0%
TTGGAAAGAGGAAGGTGATG+AGG - chr4.1:19083180-19083199 MS.gene36421:CDS 45.0%
! AGATGGACTTATCCCCTTGA+TGG - chr4.1:19082799-19082818 MS.gene36421:CDS 45.0%
! CAATGGGTGAGGATCTGTTT+TGG - chr4.1:19082507-19082526 MS.gene36421:CDS 45.0%
! TTTTGGGCTATTAGAGGAGG+TGG - chr4.1:19082524-19082543 MS.gene36421:CDS 45.0%
!! CTGTTTTGGGCTATTAGAGG+AGG - chr4.1:19082521-19082540 MS.gene36421:CDS 45.0%
ACAGTAGGGCAAATACCTGC+CGG + chr4.1:19082362-19082381 None:intergenic 50.0%
AGTCCGCTAACACCTACAGT+AGG + chr4.1:19082377-19082396 None:intergenic 50.0%
ATTCCATTCCCACACAGAGC+TGG - chr4.1:19083127-19083146 MS.gene36421:CDS 50.0%
CAGATTCGAATCCGAAGTGG+TGG - chr4.1:19082161-19082180 MS.gene36421:CDS 50.0%
GAAGAGGAGTTTCCAGAGCT+TGG - chr4.1:19082821-19082840 MS.gene36421:CDS 50.0%
GCAGGTATTTGCCCTACTGT+AGG - chr4.1:19082362-19082381 MS.gene36421:CDS 50.0%
GGAGTTTCCAGAGCTTGGTT+TGG - chr4.1:19082826-19082845 MS.gene36421:CDS 50.0%
GTCCGCTAACACCTACAGTA+GGG + chr4.1:19082376-19082395 None:intergenic 50.0%
TGCCCTACTGTAGGTGTTAG+CGG - chr4.1:19082371-19082390 MS.gene36421:CDS 50.0%
TTTCCAGCTCTGTGTGGGAA+TGG + chr4.1:19083133-19083152 None:intergenic 50.0%
! CTGCCACTTGTGAATAAGCC+TGG + chr4.1:19082654-19082673 None:intergenic 50.0%
!! GGTGTTAGCGGACTTATCAG+TGG - chr4.1:19082383-19082402 MS.gene36421:CDS 50.0%
!! GTTAGCGGACTTATCAGTGG+CGG - chr4.1:19082386-19082405 MS.gene36421:CDS 50.0%
AAAACTCTTGGCTTCCCGGC+AGG - chr4.1:19082344-19082363 MS.gene36421:CDS 55.0%
AGCAGTGCATGGGTTCAAGG+AGG - chr4.1:19082278-19082297 MS.gene36421:CDS 55.0%
AGGACAGACCTTCGTAGTCG+TGG + chr4.1:19082187-19082206 None:intergenic 55.0%
CAGTAGGGCAAATACCTGCC+GGG + chr4.1:19082361-19082380 None:intergenic 55.0%
CAGTGCATGGGTTCAAGGAG+GGG - chr4.1:19082280-19082299 MS.gene36421:CDS 55.0%
GCAGTGCATGGGTTCAAGGA+GGG - chr4.1:19082279-19082298 MS.gene36421:CDS 55.0%
TGGGCTATTAGAGGAGGTGG+AGG - chr4.1:19082527-19082546 MS.gene36421:CDS 55.0%
AGTGGTGGCCACGACTACGA+AGG - chr4.1:19082176-19082195 MS.gene36421:CDS 60.0%
CGTAGTCGTGGCCACCACTT+CGG + chr4.1:19082175-19082194 None:intergenic 60.0%
CTTATCAGTGGCGGCGGCTA+TGG - chr4.1:19082395-19082414 MS.gene36421:CDS 60.0%
GTTCAAGGAGGGGCAACTGC+TGG - chr4.1:19082290-19082309 MS.gene36421:CDS 60.0%
! AGCGGACTTATCAGTGGCGG+CGG - chr4.1:19082389-19082408 MS.gene36421:CDS 60.0%
! GGAGGTGGAGGTGCAAGCTT+TGG - chr4.1:19082539-19082558 MS.gene36421:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 19082158 19083558 19082158 ID=MS.gene36421
chr4.1 mRNA 19082158 19083558 19082158 ID=MS.gene36421.t1;Parent=MS.gene36421
chr4.1 exon 19082158 19083558 19082158 ID=MS.gene36421.t1.exon1;Parent=MS.gene36421.t1
chr4.1 CDS 19082158 19083558 19082158 ID=cds.MS.gene36421.t1;Parent=MS.gene36421.t1
Gene Sequence

>MS.gene36421

ATGCAGATTCGAATCCGAAGTGGTGGCCACGACTACGAAGGTCTGTCCTATGTTTCTGAAGTTCCATTTGTTGTCATTGATCTCATAAACCTTCAAGAAGTCCAAGTAGATGTAGAAAATAGCAGTGCATGGGTTCAAGGAGGGGCAACTGCTGGCGAACTTTTGTACAAAATTAGCCAAAAAAGCAAAACTCTTGGCTTCCCGGCAGGTATTTGCCCTACTGTAGGTGTTAGCGGACTTATCAGTGGCGGCGGCTATGGATTCTTGATGCGTAAGTTTGGTCTTTCGGCTGATAATATACTTGACGCTCACATAATTGATGTGAATGGTAGGGTTCGTGACAGAGAATCAATGGGTGAGGATCTGTTTTGGGCTATTAGAGGAGGTGGAGGTGCAAGCTTTGGAGTTATCATAGCATGGAAGATAAAACTAGTTCGTGTTCCATCAACTGTGACAATATTCAGAGTTTCTAGGACACTGGAACAAAATGCAACCAGGCTTATTCACAAGTGGCAGTTAGTAGCAAATAAGCTTGATGAGGACTTAAACATTAGGGTCATTTTGGAGAGGGTGAATACTAGTACTCAAACAAGGAAACTAACAATAAAAGCCACATTTGAATCCTTGTTTCTTGGAGGTGTAGATGGACTTATCCCCTTGATGGAAGAGGAGTTTCCAGAGCTTGGTTTGGTTAGAGAAGATTGTACTGAGATGAGTTGGATTGAATCAGTTCTCTACCATGGTGGTTTTACAAGTGATTTTACAAGTGATCAACCCCTTGAAGTTTTGTTGAATAGAACTCATAGTGGTGTGTTGTTTTTCAAGGCAAAATCTGATTATGTGAGAGAGCCTATTCCTGATATTGGGTTAGAAGGGTTATGGCCAATGTTTTATGAAAACGAGGCTAAATCTGCGGTGTTGATATTCACTCCTTATGGTGGAATAATGGATGAGATTTCGGAGTCTGAAATTCCATTCCCACACAGAGCTGGAAACATTTACCAAATTCAGCACTTAGTTTTTTGGAAAGAGGAAGGTGATGAGGTAGAAAAAAGGTATATAAATTGGATTAGAAAACTTTATAGTTACATGGAACCTTTTGTGTCAAAGTCTCCAAGAGGTGCATATGTGAATTATAGAGATCTTGATATTGGTGTGAATAATAACAATGGATACACAAGCTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTTAAGAATAACTTCAAAAGATTGGCAAAAGTGAAGACAAAAGTTGATCCTCTAAACTTTTTCAGAAATGAACAGAGTATACCTTCTCTGTCTCGTTTTGGTAACATAACAATAAGGCATAATACATTGCCTTGTGAGGAAGGGATGCAATTATTAGAGTTCCCTGGTGCTCTACATATAAAATAA
Protein sequence

>MS.gene36421.t1

MQIRIRSGGHDYEGLSYVSEVPFVVIDLINLQEVQVDVENSSAWVQGGATAGELLYKISQKSKTLGFPAGICPTVGVSGLISGGGYGFLMRKFGLSADNILDAHIIDVNGRVRDRESMGEDLFWAIRGGGGASFGVIIAWKIKLVRVPSTVTIFRVSRTLEQNATRLIHKWQLVANKLDEDLNIRVILERVNTSTQTRKLTIKATFESLFLGGVDGLIPLMEEEFPELGLVREDCTEMSWIESVLYHGGFTSDFTSDQPLEVLLNRTHSGVLFFKAKSDYVREPIPDIGLEGLWPMFYENEAKSAVLIFTPYGGIMDEISESEIPFPHRAGNIYQIQHLVFWKEEGDEVEKRYINWIRKLYSYMEPFVSKSPRGAYVNYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSLSRFGNITIRHNTLPCEEGMQLLEFPGALHIK