AlfalfaGEDB | MS.gene28723

M. sativa cultivar XinJiangDaYe / MS.gene28723

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071949 GO:0097159 GO:1901265 GO:1901363
PFAM: PF01565.26 PF08031.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28723.t1	XP_013457292.1	95.6	546	22	1	1	544	1	546	4.80E-303	1049.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28723.t1	A6P6W1	49.6	548	267	5	2	543	1	545	2.1e-153	543.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28723.t1	A0A072UQJ9	95.6	546	22	1	1	544	1	546	3.5e-303	1049.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050381	MS.gene28723	0.809572	1.77E-50	-1.69E-46
MS.gene050883	MS.gene28723	0.861362	1.08E-63	-1.69E-46
MS.gene051127	MS.gene28723	0.814722	1.33E-51	-1.69E-46
MS.gene053826	MS.gene28723	0.808332	3.26E-50	-1.69E-46
MS.gene056310	MS.gene28723	0.841536	4.48E-58	-1.69E-46
MS.gene056311	MS.gene28723	0.832767	7.84E-56	-1.69E-46
MS.gene056313	MS.gene28723	0.810188	1.31E-50	-1.69E-46
MS.gene058109	MS.gene28723	0.817408	3.35E-52	-1.69E-46
MS.gene058111	MS.gene28723	0.813307	2.73E-51	-1.69E-46
MS.gene058493	MS.gene28723	0.803897	2.80E-49	-1.69E-46
MS.gene059045	MS.gene28723	0.811285	7.56E-51	-1.69E-46
MS.gene059052	MS.gene28723	0.800036	1.74E-48	-1.69E-46
MS.gene060593	MS.gene28723	0.802404	5.70E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene28723	MS.gene36437	PPI
MS.gene35104	MS.gene28723	PPI
MS.gene28723	MS.gene28717	PPI
MS.gene35105	MS.gene28723	PPI
MS.gene36438	MS.gene28723	PPI
MS.gene28723	MS.gene36439	PPI
MS.gene003357	MS.gene28723	PPI
MS.gene28723	MS.gene28727	PPI
MS.gene28723	MS.gene63535	PPI
MS.gene28724	MS.gene28723	PPI
MS.gene51644	MS.gene28723	PPI
MS.gene28723	MS.gene51646	PPI
MS.gene28723	MS.gene009479	PPI
MS.gene28723	MS.gene36435	PPI
MS.gene009481	MS.gene28723	PPI
MS.gene028347	MS.gene28723	PPI
MS.gene28723	MS.gene35103	PPI
MS.gene02146	MS.gene28723	PPI
MS.gene28723	MS.gene028357	PPI
MS.gene63533	MS.gene28723	PPI
MS.gene28723	MS.gene56278	PPI
MS.gene28723	MS.gene028346	PPI
MS.gene35113	MS.gene28723	PPI
MS.gene28723	MS.gene028352	PPI
MS.gene36421	MS.gene28723	PPI
MS.gene28723	MS.gene28725	PPI
MS.gene28726	MS.gene28723	PPI
MS.gene28723	MS.gene35083	PPI
MS.gene35114	MS.gene28723	PPI
MS.gene028352	MS.gene28723	PPI
MS.gene28723	MS.gene047977	PPI
MS.gene63532	MS.gene28723	PPI
MS.gene36445	MS.gene28723	PPI
MS.gene28723	MS.gene35102	PPI
MS.gene28723	MS.gene28742	PPI
MS.gene028369	MS.gene28723	PPI
MS.gene28723	MS.gene35106	PPI
MS.gene28723	MS.gene028353	PPI
MS.gene28723	MS.gene56282	PPI
MS.gene28723	MS.gene51643	PPI
MS.gene28723	MS.gene36444	PPI
MS.gene28723	MS.gene028354	PPI
MS.gene28723	MS.gene56279	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28723.t1	MTR_4g094495	95.604	546	22	1	1	544	1	546	0.0	1044
MS.gene28723.t1	MTR_4g094488	82.169	544	96	1	1	544	1	543	0.0	905
MS.gene28723.t1	MTR_4g094492	80.439	547	99	4	1	544	1	542	0.0	879
MS.gene28723.t1	MTR_4g094480	79.412	544	109	2	1	544	1	541	0.0	873
MS.gene28723.t1	MTR_4g094478	79.330	537	108	2	1	537	1	534	0.0	867
MS.gene28723.t1	MTR_4g094418	71.625	437	117	2	105	537	1	434	0.0	661
MS.gene28723.t1	MTR_2g031560	53.396	530	235	5	8	536	3	521	0.0	580
MS.gene28723.t1	MTR_2g031390	52.336	535	239	7	5	536	6	527	0.0	570
MS.gene28723.t1	MTR_3g009880	55.513	526	222	6	12	536	4	518	0.0	565
MS.gene28723.t1	MTR_3g009760	53.846	533	230	6	6	536	2	520	0.0	561
MS.gene28723.t1	MTR_2g031460	57.822	505	203	5	31	535	30	524	0.0	560
MS.gene28723.t1	MTR_2g031420	55.535	533	224	7	6	536	4	525	0.0	559
MS.gene28723.t1	MTR_3g009820	53.471	533	232	6	6	536	2	520	0.0	558
MS.gene28723.t1	MTR_2g031590	52.632	532	247	4	8	537	3	531	0.0	558
MS.gene28723.t1	MTR_4g094498	55.777	502	213	3	36	537	41	533	0.0	548
MS.gene28723.t1	MTR_4g094520	51.326	528	247	6	12	537	12	531	0.0	531
MS.gene28723.t1	MTR_4g094518	54.331	508	221	6	32	536	29	528	0.0	530
MS.gene28723.t1	MTR_4g091150	51.577	539	244	6	8	536	7	538	6.76e-178	512
MS.gene28723.t1	MTR_2g031490	50.469	533	254	5	7	536	11	536	3.03e-177	511
MS.gene28723.t1	MTR_4g094515	51.316	532	252	4	7	537	624	1149	3.27e-175	525
MS.gene28723.t1	MTR_4g094515	51.866	509	235	5	33	538	39	540	2.74e-169	510
MS.gene28723.t1	MTR_4g057990	50.370	540	247	8	2	538	1	522	4.58e-175	504
MS.gene28723.t1	MTR_6g017205	50.566	530	248	6	12	539	10	527	2.21e-174	503
MS.gene28723.t1	MTR_4g091200	49.907	535	248	7	7	538	9	526	2.69e-174	503
MS.gene28723.t1	MTR_2g031510	51.976	506	233	5	34	536	40	538	1.24e-173	501
MS.gene28723.t1	MTR_4g088355	50.870	517	238	7	33	543	32	538	1.70e-172	498
MS.gene28723.t1	MTR_4g091190	48.405	533	265	4	12	543	10	533	4.49e-172	497
MS.gene28723.t1	MTR_4g091170	48.498	466	215	5	74	538	101	542	6.58e-156	457
MS.gene28723.t1	MTR_2g023590	49.574	470	228	3	69	537	4	465	6.41e-153	446
MS.gene28723.t1	MTR_1g076830	45.436	482	252	5	58	535	58	532	1.16e-132	397
MS.gene28723.t1	MTR_2g031600	59.917	242	96	1	7	247	3	244	3.38e-90	283
MS.gene28723.t1	MTR_2g031600	53.797	158	70	2	380	537	245	399	1.01e-52	184
MS.gene28723.t1	MTR_3g009850	60.444	225	85	3	313	536	5	226	9.44e-86	265
MS.gene28723.t1	MTR_2g084370	50.450	111	33	2	129	239	1	89	1.83e-32	120
MS.gene28723.t1	MTR_2g084405	58.750	80	33	0	129	208	1	80	1.35e-22	93.2
MS.gene28723.t1	MTR_8g062280	34.951	103	37	3	437	539	4	76	2.93e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28723.t1	AT5G44440	50.647	541	247	7	2	536	71	597	0.0	528
MS.gene28723.t1	AT5G44440	51.017	541	245	8	2	536	1	527	0.0	528
MS.gene28723.t1	AT5G44400	50.281	533	248	9	11	536	12	534	1.04e-179	517
MS.gene28723.t1	AT4G20820	49.174	545	254	8	2	540	1	528	1.05e-178	514
MS.gene28723.t1	AT5G44390	47.794	544	265	10	3	537	6	539	4.84e-175	505
MS.gene28723.t1	AT2G34790	47.761	536	257	9	8	539	15	531	9.62e-175	504
MS.gene28723.t1	AT5G44360	52.148	512	224	11	32	536	31	528	2.48e-171	495
MS.gene28723.t1	AT5G44360	52.344	512	223	11	32	536	47	544	3.41e-171	496
MS.gene28723.t1	AT5G44410	49.722	539	244	10	5	536	9	527	1.94e-170	493
MS.gene28723.t1	AT1G30760	49.416	514	241	8	32	539	33	533	3.45e-170	493
MS.gene28723.t1	AT1G30760	49.416	514	241	8	32	539	51	551	3.54e-170	493
MS.gene28723.t1	AT1G30700	49.341	531	256	7	9	536	4	524	3.62e-166	482
MS.gene28723.t1	AT1G26420	47.753	534	259	11	8	536	5	523	9.05e-166	481
MS.gene28723.t1	AT1G34575	47.593	540	263	8	4	539	2	525	3.22e-165	479
MS.gene28723.t1	AT1G30710	47.761	536	253	9	8	536	11	526	1.33e-164	478
MS.gene28723.t1	AT4G20860	50.380	526	233	12	22	536	19	527	1.51e-164	478
MS.gene28723.t1	AT5G44380	49.162	537	256	9	8	536	10	537	1.85e-164	478
MS.gene28723.t1	AT1G30730	47.448	529	267	6	10	536	4	523	2.41e-164	477
MS.gene28723.t1	AT5G44380	49.162	537	256	9	8	536	50	577	3.86e-164	479
MS.gene28723.t1	AT1G26410	49.405	504	245	8	34	536	53	547	8.41e-162	472
MS.gene28723.t1	AT1G26380	48.315	534	250	10	11	536	8	523	9.74e-162	471
MS.gene28723.t1	AT4G20840	47.758	513	255	7	33	542	37	539	1.44e-159	466
MS.gene28723.t1	AT1G11770	46.828	536	266	8	11	537	3	528	8.74e-159	463
MS.gene28723.t1	AT1G26390	48.911	505	250	5	33	536	28	525	2.10e-158	462
MS.gene28723.t1	AT1G26400	46.324	544	264	11	6	540	3	527	4.07e-158	461
MS.gene28723.t1	AT4G20800	47.921	505	253	5	33	536	28	523	9.00e-156	456
MS.gene28723.t1	AT1G30720	48.911	505	247	6	34	536	29	524	1.05e-154	453
MS.gene28723.t1	AT1G01980	47.758	513	250	8	33	537	31	533	3.43e-151	444
MS.gene28723.t1	AT4G20830	47.348	509	255	7	32	537	37	535	2.24e-150	442
MS.gene28723.t1	AT4G20830	47.348	509	255	7	32	537	37	535	7.84e-150	442
MS.gene28723.t1	AT1G30740	46.168	535	268	10	10	537	6	527	2.17e-145	429
MS.gene28723.t1	AT1G30740	46.355	535	267	10	10	537	9	530	2.42e-145	429
MS.gene28723.t1	AT2G34810	45.472	508	269	4	33	536	34	537	2.69e-143	424

Find 103 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTATGGTTGCTTGAATA+TGG	0.170155	4.3:+19432757	None:intergenic
GCTATAAGCAAGCTAGTATT+TGG	0.193317	4.3:-19431540	MS.gene28723:CDS
ATGCCTTCTAATCCAGTTTC+AGG	0.203252	4.3:+19431865	None:intergenic
AACTATAGAGATCTTGATAT+TGG	0.231989	4.3:-19431589	MS.gene28723:CDS
CCATATCATCATTGCATCTT+TGG	0.239092	4.3:+19431816	None:intergenic
TAGAAAACTTTATAGTTATA+TGG	0.249330	4.3:-19431650	MS.gene28723:CDS
TTATTTCAAGCTTTGTTTCT+TGG	0.263131	4.3:-19432096	MS.gene28723:CDS
GGTGGTGGAGGAGCAAGTTT+TGG	0.286201	4.3:-19432336	MS.gene28723:CDS
TCTAATCCAGTTTCAGGAAT+TGG	0.286688	4.3:+19431871	None:intergenic
AGAATTCCAATTCCTGAAAC+TGG	0.301096	4.3:-19431877	MS.gene28723:CDS
CAATGGGTGAGGATTTGTTT+TGG	0.303294	4.3:-19432368	MS.gene28723:CDS
ACTATAGAGATCTTGATATT+GGG	0.314135	4.3:-19431588	MS.gene28723:CDS
GTGACAATTACAAGAGGTTT+TGG	0.315638	4.3:+19432795	None:intergenic
CACTTCAGTGGTGGTGGATA+TGG	0.317127	4.3:-19432480	MS.gene28723:CDS
GAACCATCTTCATCAAATAA+AGG	0.318041	4.3:+19431838	None:intergenic
CCAAAGATGCAATGATGATA+TGG	0.328286	4.3:-19431816	MS.gene28723:CDS
TCATTTCTGAAGAAGTTTAG+AGG	0.331220	4.3:+19431457	None:intergenic
ACTTCAGGTGTAGAACCTTT+TGG	0.341232	4.3:+19431958	None:intergenic
ACAGTAGGACAAACACTTGC+AGG	0.361034	4.3:+19432516	None:intergenic
TTGAGTTATGTGAATAACTA+TGG	0.361143	4.3:+19432916	None:intergenic
GGAACCTTGTTGCGTAAATA+TGG	0.364610	4.3:-19432459	MS.gene28723:CDS
TATTGATGTGTAAGAGGAAT+TGG	0.365680	4.3:+19432863	None:intergenic
CTATAAGCAAGCTAGTATTT+GGG	0.368691	4.3:-19431539	MS.gene28723:CDS
AATTGGAATTCTCACAAAAT+CGG	0.370976	4.3:+19431888	None:intergenic
AATCTGTAGGCCATGGTATT+GGG	0.371585	4.3:+19432731	None:intergenic
CTTGGTGACTTTGAAACAAA+AGG	0.372572	4.3:+19431625	None:intergenic
TGATTCAATACTCGGTTCAT+TGG	0.373369	4.3:-19431717	MS.gene28723:CDS
GTTTCCATATGGTGGCAAAA+TGG	0.373437	4.3:-19431794	MS.gene28723:CDS
ATTCCTGAAACTGGATTAGA+AGG	0.376708	4.3:-19431868	MS.gene28723:CDS
GAATCTGTAGGCCATGGTAT+TGG	0.381926	4.3:+19432730	None:intergenic
TTTCCACTTCTATGTGGAAA+TGG	0.387520	4.3:+19431748	None:intergenic
ATGGTATTGGGAACAGATTA+TGG	0.392865	4.3:+19432743	None:intergenic
GTAAACACAGTAACAATTGA+TGG	0.397213	4.3:+19432276	None:intergenic
TCATTTACCTCTTCAGCAAT+TGG	0.403207	4.3:-19432978	MS.gene28723:CDS
AAGCGGCGGCCACGACTTTG+AGG	0.408872	4.3:-19432700	MS.gene28723:CDS
CAAGAGAAGTTTCCTGAGCT+TGG	0.427109	4.3:-19432045	MS.gene28723:CDS
ATGAGATCAATGACGACAAA+TGG	0.430921	4.3:+19432654	None:intergenic
TGGGCTATTAGGGGTGGTGG+AGG	0.435638	4.3:-19432348	MS.gene28723:CDS
GATTATGGTTGCTTGAATAT+GGG	0.436969	4.3:+19432758	None:intergenic
GAAGTTTCCTGAGCTTGGTT+TGG	0.437875	4.3:-19432040	MS.gene28723:CDS
CTTATTCATGATTCAATACT+CGG	0.438411	4.3:-19431725	MS.gene28723:CDS
AATATTGTATTGAAATGAGT+TGG	0.439258	4.3:-19432011	MS.gene28723:CDS
TTTCAAGCTTTGTTTCTTGG+AGG	0.440275	4.3:-19432093	MS.gene28723:CDS
TGGCCTTTATTTGATGAAGA+TGG	0.441205	4.3:-19431841	MS.gene28723:CDS
TATACCTTCTCATGTGTTCA+AGG	0.466922	4.3:-19431428	MS.gene28723:CDS
GCTTGAAATAAAACTAGTAC+TGG	0.469012	4.3:+19432108	None:intergenic
ACTTCAAGAATAACTTCAAA+AGG	0.469443	4.3:-19431507	MS.gene28723:CDS
TTGGAGTATTGATGTGTAAG+AGG	0.474833	4.3:+19432857	None:intergenic
GTTCTGTTAAGCAACACTTC+AGG	0.475413	4.3:+19431943	None:intergenic
CAAGAATAACTTCAAAAGGT+TGG	0.477785	4.3:-19431503	MS.gene28723:CDS
GTGAGCATCAATGATGTTAT+CGG	0.478746	4.3:+19432428	None:intergenic
AAAACTGCATGGGTTCAATC+TGG	0.487082	4.3:-19432597	MS.gene28723:CDS
TGTGTAAGAGGAATTGGCTT+TGG	0.503548	4.3:+19432869	None:intergenic
AGTAGTACCAATTGCTGAAG+AGG	0.507421	4.3:+19432971	None:intergenic
AAACTGGATTAGAAGGCATA+TGG	0.508202	4.3:-19431861	MS.gene28723:CDS
GATGAATATTGTCTGGCAAA+GGG	0.514394	4.3:-19432169	MS.gene28723:CDS
ATATAGAGATGAATATTGTC+TGG	0.517958	4.3:-19432176	MS.gene28723:CDS
ATTCCATTTCCACATAGAAG+TGG	0.522494	4.3:-19431751	MS.gene28723:CDS
AATAACTATGGAGACATTGA+AGG	0.523986	4.3:+19432928	None:intergenic
TCTCTAACCAAACCAAGCTC+AGG	0.524526	4.3:+19432033	None:intergenic
ATAGACAAATATGAGCTTCG+AGG	0.525149	4.3:+19433023	None:intergenic
AGCGGCGGCCACGACTTTGA+GGG	0.526742	4.3:-19432699	MS.gene28723:CDS
TGTCCTACTGTAGGTGTTGG+TGG	0.531106	4.3:-19432504	MS.gene28723:CDS
GTTTCTTGACAGAGAAGCAA+TGG	0.534469	4.3:-19432385	MS.gene28723:CDS
AGATGAATATTGTCTGGCAA+AGG	0.537229	4.3:-19432170	MS.gene28723:CDS
AATAAGTTTCCACTTCTATG+TGG	0.541507	4.3:+19431742	None:intergenic
CTATAGAGATCTTGATATTG+GGG	0.546784	4.3:-19431587	MS.gene28723:CDS
TAGTTAATATATGCAGCTCT+TGG	0.560303	4.3:+19431607	None:intergenic
GCAAGTGTTTGTCCTACTGT+AGG	0.562475	4.3:-19432513	MS.gene28723:CDS
TTTCTTGACAGAGAAGCAAT+GGG	0.565816	4.3:-19432384	MS.gene28723:CDS
AGGAAACTTCTCTTGAACCA+AGG	0.566167	4.3:+19432053	None:intergenic
GGTGGACACTTCAGTGGTGG+TGG	0.566542	4.3:-19432486	MS.gene28723:CDS
TGTAGATAAACTCATACCCT+TGG	0.566543	4.3:-19432070	MS.gene28723:CDS
GTTTGTCCTACTGTAGGTGT+TGG	0.572069	4.3:-19432507	MS.gene28723:CDS
ATTGGGGTGAATAATAACAA+TGG	0.572947	4.3:-19431571	MS.gene28723:CDS
CAACAAAGCTTGTTCACAAG+TGG	0.578289	4.3:-19432227	MS.gene28723:CDS
TGCTCACATAATTGATGTGA+AGG	0.585552	4.3:-19432412	MS.gene28723:CDS
GGAAACTTCTCTTGAACCAA+GGG	0.586930	4.3:+19432054	None:intergenic
TGACAGAGAAGCAATGGGTG+AGG	0.588886	4.3:-19432379	MS.gene28723:CDS
ACATAATTGATGTGAAGGGT+AGG	0.589985	4.3:-19432407	MS.gene28723:CDS
TGAATATGGGATATTTCTAG+AGG	0.601628	4.3:+19432771	None:intergenic
TGTCCCTTGAACACATGAGA+AGG	0.607142	4.3:+19431424	None:intergenic
AAGACCATATTTACGCAACA+AGG	0.610440	4.3:+19432455	None:intergenic
GTTCAATCTGGTGCAACACT+TGG	0.613052	4.3:-19432585	MS.gene28723:CDS
TCGGTTCATTGGCATCAAGA+AGG	0.613335	4.3:-19431706	MS.gene28723:CDS
CAGTAGGACAAACACTTGCA+GGG	0.613601	4.3:+19432517	None:intergenic
GCATCAAGAAGGTGATGAGG+TGG	0.615161	4.3:-19431695	MS.gene28723:CDS
ATGATGATATGGTTTCCATA+TGG	0.616039	4.3:-19431805	MS.gene28723:CDS
GAATTCGAATCTGTAGGCCA+TGG	0.621127	4.3:+19432724	None:intergenic
ATACCTTCTCATGTGTTCAA+GGG	0.623373	4.3:-19431427	MS.gene28723:CDS
TATAAGCAAGCTAGTATTTG+GGG	0.624423	4.3:-19431538	MS.gene28723:CDS
GTTGGTGGACACTTCAGTGG+TGG	0.625730	4.3:-19432489	MS.gene28723:CDS
GGTGTTGGTGGACACTTCAG+TGG	0.638405	4.3:-19432492	MS.gene28723:CDS
AGAGGTGTGACAATTACAAG+AGG	0.647102	4.3:+19432789	None:intergenic
ATGATATGGTTTCCATATGG+TGG	0.655336	4.3:-19431802	MS.gene28723:CDS
TTGGCATCAAGAAGGTGATG+AGG	0.656241	4.3:-19431698	MS.gene28723:CDS
CAGATTCGAATTCGAAGCGG+CGG	0.660883	4.3:-19432714	MS.gene28723:CDS
TGTCCACCAACACCTACAGT+AGG	0.663218	4.3:+19432501	None:intergenic
ATAATCTGTTCCCAATACCA+TGG	0.671513	4.3:-19432741	MS.gene28723:CDS
AAGACAGACCCTCAAAGTCG+TGG	0.674602	4.3:+19432691	None:intergenic
CGCTTCGAATTCGAATCTGT+AGG	0.689715	4.3:+19432718	None:intergenic
GCTCACATAATTGATGTGAA+GGG	0.694549	4.3:-19432411	MS.gene28723:CDS
CTACAGATTCGAATTCGAAG+CGG	0.715605	4.3:-19432717	MS.gene28723:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTAAATTCAAGTAAAAA+AGG	-	chr4.3:19432302-19432321	MS.gene28723:CDS	10.0%
!!	TAGAAAACTTTATAGTTATA+TGG	-	chr4.3:19432796-19432815	MS.gene28723:CDS	15.0%
!!	AATATTGTATTGAAATGAGT+TGG	-	chr4.3:19432435-19432454	MS.gene28723:CDS	20.0%
!	AACTATAGAGATCTTGATAT+TGG	-	chr4.3:19432857-19432876	MS.gene28723:CDS	25.0%
!	AATTGGAATTCTCACAAAAT+CGG	+	chr4.3:19432561-19432580	None:intergenic	25.0%
!	ACTATAGAGATCTTGATATT+GGG	-	chr4.3:19432858-19432877	MS.gene28723:CDS	25.0%
!	ACTTCAAGAATAACTTCAAA+AGG	-	chr4.3:19432939-19432958	MS.gene28723:CDS	25.0%
!	ATATAGAGATGAATATTGTC+TGG	-	chr4.3:19432270-19432289	MS.gene28723:CDS	25.0%
!	CTTATTCATGATTCAATACT+CGG	-	chr4.3:19432721-19432740	MS.gene28723:CDS	25.0%
!	TTGAGTTATGTGAATAACTA+TGG	+	chr4.3:19431533-19431552	None:intergenic	25.0%
!!!	TACTTTTTTCAGTTTCCAAA+AGG	-	chr4.3:19432473-19432492	MS.gene28723:CDS	25.0%
!!!	TTATTTCAAGCTTTGTTTCT+TGG	-	chr4.3:19432350-19432369	MS.gene28723:CDS	25.0%
	AATAACTATGGAGACATTGA+AGG	+	chr4.3:19431521-19431540	None:intergenic	30.0%
	AATAAGTTTCCACTTCTATG+TGG	+	chr4.3:19432707-19432726	None:intergenic	30.0%
	AGATTATGGTTGCTTGAATA+TGG	+	chr4.3:19431692-19431711	None:intergenic	30.0%
	ATGATGATATGGTTTCCATA+TGG	-	chr4.3:19432641-19432660	MS.gene28723:CDS	30.0%
	ATGTACACAAAAAAACTGCA+TGG	-	chr4.3:19431838-19431857	MS.gene28723:CDS	30.0%
	ATTGGGGTGAATAATAACAA+TGG	-	chr4.3:19432875-19432894	MS.gene28723:CDS	30.0%
	CAAGAATAACTTCAAAAGGT+TGG	-	chr4.3:19432943-19432962	MS.gene28723:CDS	30.0%
	CTATAAGCAAGCTAGTATTT+GGG	-	chr4.3:19432907-19432926	MS.gene28723:CDS	30.0%
	CTATAGAGATCTTGATATTG+GGG	-	chr4.3:19432859-19432878	MS.gene28723:CDS	30.0%
	GAACCATCTTCATCAAATAA+AGG	+	chr4.3:19432611-19432630	None:intergenic	30.0%
	GATTATGGTTGCTTGAATAT+GGG	+	chr4.3:19431691-19431710	None:intergenic	30.0%
	GCTTGAAATAAAACTAGTAC+TGG	+	chr4.3:19432341-19432360	None:intergenic	30.0%
	GTAAACACAGTAACAATTGA+TGG	+	chr4.3:19432173-19432192	None:intergenic	30.0%
	GTTTACTGTTCCAAAAACAT+TGG	-	chr4.3:19432187-19432206	MS.gene28723:CDS	30.0%
	TAGTTAATATATGCAGCTCT+TGG	+	chr4.3:19432842-19432861	None:intergenic	30.0%
	TATAAGCAAGCTAGTATTTG+GGG	-	chr4.3:19432908-19432927	MS.gene28723:CDS	30.0%
	TATTGATGTGTAAGAGGAAT+TGG	+	chr4.3:19431586-19431605	None:intergenic	30.0%
	TGGAAAAATTGCACATCAAT+TGG	-	chr4.3:19432771-19432790	MS.gene28723:CDS	30.0%
	TGTACACAAAAAAACTGCAT+GGG	-	chr4.3:19431839-19431858	MS.gene28723:CDS	30.0%
!	GCATTTTGTTCCAATGTTTT+TGG	+	chr4.3:19432200-19432219	None:intergenic	30.0%
!	TCATTTCTGAAGAAGTTTAG+AGG	+	chr4.3:19432992-19433011	None:intergenic	30.0%
!!	TGAATATGGGATATTTCTAG+AGG	+	chr4.3:19431678-19431697	None:intergenic	30.0%
!!!	AACCTAGAGTTTTGCTTTTT+TGG	+	chr4.3:19431908-19431927	None:intergenic	30.0%
!!!	TGAGATTTTGAGTTGAGAAT+TGG	+	chr4.3:19431611-19431630	None:intergenic	30.0%
	AAGACCATATTTACGCAACA+AGG	+	chr4.3:19431994-19432013	None:intergenic	35.0%
	ACATAATTGATGTGAAGGGT+AGG	-	chr4.3:19432039-19432058	MS.gene28723:CDS	35.0%
	AGAATTCCAATTCCTGAAAC+TGG	-	chr4.3:19432569-19432588	MS.gene28723:CDS	35.0%
	AGATGAATATTGTCTGGCAA+AGG	-	chr4.3:19432276-19432295	MS.gene28723:CDS	35.0%
	AGCCAAAAAAGCAAAACTCT+AGG	-	chr4.3:19431903-19431922	MS.gene28723:CDS	35.0%
	ATAATCTGTTCCCAATACCA+TGG	-	chr4.3:19431705-19431724	MS.gene28723:CDS	35.0%
	ATACCTTCTCATGTGTTCAA+GGG	-	chr4.3:19433019-19433038	MS.gene28723:CDS	35.0%
	ATAGACAAATATGAGCTTCG+AGG	+	chr4.3:19431426-19431445	None:intergenic	35.0%
	ATGAGATCAATGACGACAAA+TGG	+	chr4.3:19431795-19431814	None:intergenic	35.0%
	ATGATATGGTTTCCATATGG+TGG	-	chr4.3:19432644-19432663	MS.gene28723:CDS	35.0%
	ATTCCATTTCCACATAGAAG+TGG	-	chr4.3:19432695-19432714	MS.gene28723:CDS	35.0%
	CCAAAGATGCAATGATGATA+TGG	-	chr4.3:19432630-19432649	MS.gene28723:CDS	35.0%
	CCATATCATCATTGCATCTT+TGG	+	chr4.3:19432633-19432652	None:intergenic	35.0%
	GATGAATATTGTCTGGCAAA+GGG	-	chr4.3:19432277-19432296	MS.gene28723:CDS	35.0%
	GCTATAAGCAAGCTAGTATT+TGG	-	chr4.3:19432906-19432925	MS.gene28723:CDS	35.0%
	GCTCACATAATTGATGTGAA+GGG	-	chr4.3:19432035-19432054	MS.gene28723:CDS	35.0%
	GTGAGCATCAATGATGTTAT+CGG	+	chr4.3:19432021-19432040	None:intergenic	35.0%
	TATACCTTCTCATGTGTTCA+AGG	-	chr4.3:19433018-19433037	MS.gene28723:CDS	35.0%
	TCATTTACCTCTTCAGCAAT+TGG	-	chr4.3:19431468-19431487	MS.gene28723:CDS	35.0%
	TCTAATCCAGTTTCAGGAAT+TGG	+	chr4.3:19432578-19432597	None:intergenic	35.0%
	TGATTCAATACTCGGTTCAT+TGG	-	chr4.3:19432729-19432748	MS.gene28723:CDS	35.0%
	TGCTCACATAATTGATGTGA+AGG	-	chr4.3:19432034-19432053	MS.gene28723:CDS	35.0%
	TGTAGATAAACTCATACCCT+TGG	-	chr4.3:19432376-19432395	MS.gene28723:CDS	35.0%
	TTTCCACTTCTATGTGGAAA+TGG	+	chr4.3:19432701-19432720	None:intergenic	35.0%
	TTTCTTGACAGAGAAGCAAT+GGG	-	chr4.3:19432062-19432081	MS.gene28723:CDS	35.0%
!	AAACTGGATTAGAAGGCATA+TGG	-	chr4.3:19432585-19432604	MS.gene28723:CDS	35.0%
!	ATGGTATTGGGAACAGATTA+TGG	+	chr4.3:19431706-19431725	None:intergenic	35.0%
!	ATTCCTGAAACTGGATTAGA+AGG	-	chr4.3:19432578-19432597	MS.gene28723:CDS	35.0%
!	GTGACAATTACAAGAGGTTT+TGG	+	chr4.3:19431654-19431673	None:intergenic	35.0%
!	TGGCCTTTATTTGATGAAGA+TGG	-	chr4.3:19432605-19432624	MS.gene28723:CDS	35.0%
!	TGTTCACAAGTGGCAATTTT+TGG	-	chr4.3:19432229-19432248	MS.gene28723:CDS	35.0%
!	TTATCCATTTTGCCACCATA+TGG	+	chr4.3:19432659-19432678	None:intergenic	35.0%
!!	AATGGGTGAGGATTTGTTTT+GGG	-	chr4.3:19432079-19432098	MS.gene28723:CDS	35.0%
!!	CTTGGTGACTTTGAAACAAA+AGG	+	chr4.3:19432824-19432843	None:intergenic	35.0%
!!	TTGGAGTATTGATGTGTAAG+AGG	+	chr4.3:19431592-19431611	None:intergenic	35.0%
!!	TTTCAAGCTTTGTTTCTTGG+AGG	-	chr4.3:19432353-19432372	MS.gene28723:CDS	35.0%
!!!	AGGATTTGTTTTGGGCTATT+AGG	-	chr4.3:19432087-19432106	MS.gene28723:CDS	35.0%
!!!	GATTTGTTTTGGGCTATTAG+GGG	-	chr4.3:19432089-19432108	MS.gene28723:CDS	35.0%
!!!	GGATTTGTTTTGGGCTATTA+GGG	-	chr4.3:19432088-19432107	MS.gene28723:CDS	35.0%
!!!	GTTTTGGTGTCATAGTTTCA+TGG	-	chr4.3:19432126-19432145	MS.gene28723:CDS	35.0%
	AAAACTGCATGGGTTCAATC+TGG	-	chr4.3:19431849-19431868	MS.gene28723:CDS	40.0%
	AATCTGTAGGCCATGGTATT+GGG	+	chr4.3:19431718-19431737	None:intergenic	40.0%
	AGGAAACTTCTCTTGAACCA+AGG	+	chr4.3:19432396-19432415	None:intergenic	40.0%
	AGTAGTACCAATTGCTGAAG+AGG	+	chr4.3:19431478-19431497	None:intergenic	40.0%
	ATGCCTTCTAATCCAGTTTC+AGG	+	chr4.3:19432584-19432603	None:intergenic	40.0%
	CTACAGATTCGAATTCGAAG+CGG	-	chr4.3:19431729-19431748	MS.gene28723:CDS	40.0%
	GGAAACTTCTCTTGAACCAA+GGG	+	chr4.3:19432395-19432414	None:intergenic	40.0%
	GGAACCTTGTTGCGTAAATA+TGG	-	chr4.3:19431987-19432006	MS.gene28723:CDS	40.0%
	GTTCTGTTAAGCAACACTTC+AGG	+	chr4.3:19432506-19432525	None:intergenic	40.0%
	GTTTCCATATGGTGGCAAAA+TGG	-	chr4.3:19432652-19432671	MS.gene28723:CDS	40.0%
	GTTTCTTGACAGAGAAGCAA+TGG	-	chr4.3:19432061-19432080	MS.gene28723:CDS	40.0%
!	ACTTCAGGTGTAGAACCTTT+TGG	+	chr4.3:19432491-19432510	None:intergenic	40.0%
!	AGAGGTGTGACAATTACAAG+AGG	+	chr4.3:19431660-19431679	None:intergenic	40.0%
!	CAACAAAGCTTGTTCACAAG+TGG	-	chr4.3:19432219-19432238	MS.gene28723:CDS	40.0%
!	TGTGTAAGAGGAATTGGCTT+TGG	+	chr4.3:19431580-19431599	None:intergenic	40.0%
!!	CAATGGGTGAGGATTTGTTT+TGG	-	chr4.3:19432078-19432097	MS.gene28723:CDS	40.0%
!!!	AAGAGGTTTTGGTGTTGTGT+TGG	+	chr4.3:19431643-19431662	None:intergenic	40.0%
	ACAGTAGGACAAACACTTGC+AGG	+	chr4.3:19431933-19431952	None:intergenic	45.0%
	CAAGAGAAGTTTCCTGAGCT+TGG	-	chr4.3:19432401-19432420	MS.gene28723:CDS	45.0%
	CAGTAGGACAAACACTTGCA+GGG	+	chr4.3:19431932-19431951	None:intergenic	45.0%
	CGCTTCGAATTCGAATCTGT+AGG	+	chr4.3:19431731-19431750	None:intergenic	45.0%
	GAAGTTTCCTGAGCTTGGTT+TGG	-	chr4.3:19432406-19432425	MS.gene28723:CDS	45.0%
	GAATCTGTAGGCCATGGTAT+TGG	+	chr4.3:19431719-19431738	None:intergenic	45.0%
	GAATTCGAATCTGTAGGCCA+TGG	+	chr4.3:19431725-19431744	None:intergenic	45.0%
	GCAAGTGTTTGTCCTACTGT+AGG	-	chr4.3:19431933-19431952	MS.gene28723:CDS	45.0%
	GTTCAATCTGGTGCAACACT+TGG	-	chr4.3:19431861-19431880	MS.gene28723:CDS	45.0%
	GTTTGTCCTACTGTAGGTGT+TGG	-	chr4.3:19431939-19431958	MS.gene28723:CDS	45.0%
	TCGGTTCATTGGCATCAAGA+AGG	-	chr4.3:19432740-19432759	MS.gene28723:CDS	45.0%
	TCTCTAACCAAACCAAGCTC+AGG	+	chr4.3:19432416-19432435	None:intergenic	45.0%
	TGTCCCTTGAACACATGAGA+AGG	+	chr4.3:19433025-19433044	None:intergenic	45.0%
	TTGGCATCAAGAAGGTGATG+AGG	-	chr4.3:19432748-19432767	MS.gene28723:CDS	45.0%
!!!	TTGTTTTGGGCTATTAGGGG+TGG	-	chr4.3:19432092-19432111	MS.gene28723:CDS	45.0%
	AAGACAGACCCTCAAAGTCG+TGG	+	chr4.3:19431758-19431777	None:intergenic	50.0%
	CAGATTCGAATTCGAAGCGG+CGG	-	chr4.3:19431732-19431751	MS.gene28723:CDS	50.0%
	GCATCAAGAAGGTGATGAGG+TGG	-	chr4.3:19432751-19432770	MS.gene28723:CDS	50.0%
	TGACAGAGAAGCAATGGGTG+AGG	-	chr4.3:19432067-19432086	MS.gene28723:CDS	50.0%
	TGTCCACCAACACCTACAGT+AGG	+	chr4.3:19431948-19431967	None:intergenic	50.0%
!	TTTTGGGCTATTAGGGGTGG+TGG	-	chr4.3:19432095-19432114	MS.gene28723:CDS	50.0%
!!	CACTTCAGTGGTGGTGGATA+TGG	-	chr4.3:19431966-19431985	MS.gene28723:CDS	50.0%
!!	TGTCCTACTGTAGGTGTTGG+TGG	-	chr4.3:19431942-19431961	MS.gene28723:CDS	50.0%
!	GTTGGTGGACACTTCAGTGG+TGG	-	chr4.3:19431957-19431976	MS.gene28723:CDS	55.0%
!!	GGTGTTGGTGGACACTTCAG+TGG	-	chr4.3:19431954-19431973	MS.gene28723:CDS	55.0%
!!!	GGTGGTGGAGGAGCAAGTTT+TGG	-	chr4.3:19432110-19432129	MS.gene28723:CDS	55.0%
	GGTGGACACTTCAGTGGTGG+TGG	-	chr4.3:19431960-19431979	MS.gene28723:CDS	60.0%
!	TGGGCTATTAGGGGTGGTGG+AGG	-	chr4.3:19432098-19432117	MS.gene28723:CDS	60.0%
	AAGCGGCGGCCACGACTTTG+AGG	-	chr4.3:19431746-19431765	MS.gene28723:CDS	65.0%
	AGCGGCGGCCACGACTTTGA+GGG	-	chr4.3:19431747-19431766	MS.gene28723:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	19431417	19433051	19431417	ID=MS.gene28723
chr4.3	mRNA	19431417	19433051	19431417	ID=MS.gene28723.t1;Parent=MS.gene28723
chr4.3	exon	19431417	19433051	19431417	ID=MS.gene28723.t1.exon1;Parent=MS.gene28723.t1
chr4.3	CDS	19431417	19433051	19431417	ID=cds.MS.gene28723.t1;Parent=MS.gene28723.t1

Gene Sequence

>MS.gene28723

ATGATGCCTCGAAGCTCATATTTGTCTATTATACTAATTGCTATTTTATTTTCATTTACCTCTTCAGCAATTGGTACTACTACTAATGAAGATAATAACTTCCTTCAATGTCTCCATAGTTATTCACATAACTCAACTTCAATCTCTGAAGTTGTGTACACCAAAGCCAATTCCTCTTACACATCAATACTCCAATTCTCAACTCAAAATCTCAGATTTGCAACCAACACAACACCAAAACCTCTTGTAATTGTCACACCTCTAGAAATATCCCATATTCAAGCAACCATAATCTGTTCCCAATACCATGGCCTACAGATTCGAATTCGAAGCGGCGGCCACGACTTTGAGGGTCTGTCTTATGTCTCTGAAGTTCCATTTGTCGTCATTGATCTCATAAACTTCAGAGAAATTGAAATAAATGTACACAAAAAAACTGCATGGGTTCAATCTGGTGCAACACTTGGTGAACTTTACTACGCAATTAGCCAAAAAAGCAAAACTCTAGGTTTCCCTGCAAGTGTTTGTCCTACTGTAGGTGTTGGTGGACACTTCAGTGGTGGTGGATATGGAACCTTGTTGCGTAAATATGGTCTTGCAGCCGATAACATCATTGATGCTCACATAATTGATGTGAAGGGTAGGTTTCTTGACAGAGAAGCAATGGGTGAGGATTTGTTTTGGGCTATTAGGGGTGGTGGAGGAGCAAGTTTTGGTGTCATAGTTTCATGGAAGATTAAACTTGTTCAAGTTCCATCAATTGTTACTGTGTTTACTGTTCCAAAAACATTGGAACAAAATGCAACAAAGCTTGTTCACAAGTGGCAATTTTTGGCAAGTAAACTTGATGAAAATATAGAGATGAATATTGTCTGGCAAAGGGTAAATTTAAATTCAAGTAAAAAAGGTGAGTCAAAGTCACCAGTACTAGTTTTATTTCAAGCTTTGTTTCTTGGAGGTGTAGATAAACTCATACCCTTGGTTCAAGAGAAGTTTCCTGAGCTTGGTTTGGTTAGAGAATATTGTATTGAAATGAGTTGGATTGAATCTGTTCTTTACTTTTTTCAGTTTCCAAAAGGTTCTACACCTGAAGTGTTGCTTAACAGAACACAAGCTACACTTGATATTTTCAAAGCAAAATCCGATTTTGTGAGAATTCCAATTCCTGAAACTGGATTAGAAGGCATATGGCCTTTATTTGATGAAGATGGTTCCAAAGATGCAATGATGATATGGTTTCCATATGGTGGCAAAATGGATAATATTTCAGAGTCTGAAATTCCATTTCCACATAGAAGTGGAAACTTATTCATGATTCAATACTCGGTTCATTGGCATCAAGAAGGTGATGAGGTGGAAAAATTGCACATCAATTGGATTAGAAAACTTTATAGTTATATGGAACCTTTTGTTTCAAAGTCACCAAGAGCTGCATATATTAACTATAGAGATCTTGATATTGGGGTGAATAATAACAATGGATACACAAGCTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTCAAGAATAACTTCAAAAGGTTGGCAAAAGTGAAGACAAAAGTTGATCCTCTAAACTTCTTCAGAAATGAACAGAGTATACCTTCTCATGTGTTCAAGGGACACAAATAG

Protein sequence

>MS.gene28723.t1

MMPRSSYLSIILIAILFSFTSSAIGTTTNEDNNFLQCLHSYSHNSTSISEVVYTKANSSYTSILQFSTQNLRFATNTTPKPLVIVTPLEISHIQATIICSQYHGLQIRIRSGGHDFEGLSYVSEVPFVVIDLINFREIEINVHKKTAWVQSGATLGELYYAISQKSKTLGFPASVCPTVGVGGHFSGGGYGTLLRKYGLAADNIIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIKLVQVPSIVTVFTVPKTLEQNATKLVHKWQFLASKLDENIEMNIVWQRVNLNSSKKGESKSPVLVLFQALFLGGVDKLIPLVQEKFPELGLVREYCIEMSWIESVLYFFQFPKGSTPEVLLNRTQATLDIFKAKSDFVRIPIPETGLEGIWPLFDEDGSKDAMMIWFPYGGKMDNISESEIPFPHRSGNLFMIQYSVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSHVFKGHK