AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35083

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35083.t1 MTR_4g094418 95.259 464 22 0 1 464 1 464 0.0 918
MS.gene35083.t1 MTR_4g094488 73.684 437 108 3 1 434 104 536 0.0 649
MS.gene35083.t1 MTR_4g094478 71.789 436 118 2 1 435 104 535 0.0 643
MS.gene35083.t1 MTR_4g094495 70.938 437 120 2 1 434 107 539 0.0 632
MS.gene35083.t1 MTR_4g094492 69.300 443 128 3 1 440 104 541 0.0 626
MS.gene35083.t1 MTR_4g094480 71.034 435 121 3 1 434 104 534 0.0 625
MS.gene35083.t1 MTR_3g009820 59.174 436 168 4 1 436 98 523 0.0 528
MS.gene35083.t1 MTR_3g009760 58.716 436 170 4 1 436 98 523 0.0 527
MS.gene35083.t1 MTR_3g009880 58.202 445 173 5 1 445 96 527 0.0 521
MS.gene35083.t1 MTR_2g031560 55.982 443 188 3 1 443 96 531 1.05e-180 517
MS.gene35083.t1 MTR_2g031390 54.691 437 188 5 1 435 101 529 6.71e-179 512
MS.gene35083.t1 MTR_2g031590 57.207 444 179 5 1 440 101 537 9.79e-177 506
MS.gene35083.t1 MTR_2g031460 58.969 446 172 5 1 446 104 538 5.92e-176 504
MS.gene35083.t1 MTR_2g031420 58.028 436 172 5 1 436 104 528 4.67e-173 496
MS.gene35083.t1 MTR_4g094498 59.404 436 167 4 1 436 110 535 5.85e-173 497
MS.gene35083.t1 MTR_4g094520 54.233 437 189 6 1 435 105 532 8.12e-165 476
MS.gene35083.t1 MTR_4g094518 55.378 437 185 5 1 435 102 530 7.80e-164 473
MS.gene35083.t1 MTR_2g031490 52.184 435 204 3 1 435 108 538 4.31e-157 456
MS.gene35083.t1 MTR_2g031510 51.945 437 206 3 1 437 110 542 2.46e-155 452
MS.gene35083.t1 MTR_4g094515 54.023 435 193 3 1 435 723 1150 6.51e-155 469
MS.gene35083.t1 MTR_4g094515 51.259 437 202 4 1 434 111 539 7.99e-144 441
MS.gene35083.t1 MTR_4g088355 52.847 439 195 5 1 436 105 534 7.76e-151 440
MS.gene35083.t1 MTR_4g091150 52.164 439 198 6 1 435 110 540 6.59e-150 438
MS.gene35083.t1 MTR_4g091200 51.716 437 203 4 1 436 98 527 8.87e-150 437
MS.gene35083.t1 MTR_4g091190 51.034 435 203 4 1 434 102 527 1.87e-149 436
MS.gene35083.t1 MTR_4g091170 49.309 434 196 4 1 434 132 541 2.10e-149 438
MS.gene35083.t1 MTR_4g057990 51.030 437 203 4 1 436 97 523 1.53e-147 431
MS.gene35083.t1 MTR_6g017205 51.494 435 203 4 1 434 98 525 3.40e-147 431
MS.gene35083.t1 MTR_2g023590 49.203 439 213 4 1 438 40 469 4.39e-141 413
MS.gene35083.t1 MTR_1g076830 45.909 440 226 7 1 436 105 536 5.64e-122 367
MS.gene35083.t1 MTR_3g009850 57.447 235 94 3 202 436 1 229 1.20e-90 275
MS.gene35083.t1 MTR_2g031600 71.329 143 41 0 1 143 102 244 3.59e-62 207
MS.gene35083.t1 MTR_2g031600 53.216 171 76 3 271 440 238 405 3.43e-55 189
MS.gene35083.t1 MTR_2g084370 48.649 111 35 2 25 135 1 89 6.93e-31 115
MS.gene35083.t1 MTR_2g084405 52.500 80 38 0 25 104 1 80 1.06e-19 84.7
MS.gene35083.t1 MTR_8g062280 35.354 99 34 3 334 432 4 72 6.15e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35083.t1 AT4G20820 54.128 436 184 5 1 436 108 527 1.01e-165 478
MS.gene35083.t1 AT1G30760 53.670 436 188 5 1 434 126 549 5.84e-165 477
MS.gene35083.t1 AT1G30760 53.670 436 188 5 1 434 108 531 8.22e-165 476
MS.gene35083.t1 AT2G34790 54.670 439 179 8 1 434 106 529 7.33e-164 473
MS.gene35083.t1 AT5G44360 53.881 438 184 7 1 435 108 530 2.25e-163 472
MS.gene35083.t1 AT5G44360 53.881 438 184 7 1 435 124 546 3.27e-163 472
MS.gene35083.t1 AT5G44440 52.834 441 193 5 1 437 102 531 1.34e-162 470
MS.gene35083.t1 AT5G44440 52.834 441 193 5 1 437 172 601 8.70e-162 471
MS.gene35083.t1 AT4G20860 52.523 436 195 5 1 435 105 529 6.37e-161 466
MS.gene35083.t1 AT5G44400 52.620 439 194 7 1 435 108 536 3.80e-160 464
MS.gene35083.t1 AT5G44390 51.481 439 201 7 1 435 110 540 6.79e-159 461
MS.gene35083.t1 AT5G44380 53.986 439 188 7 1 435 111 539 2.72e-155 452
MS.gene35083.t1 AT5G44380 53.986 439 188 7 1 435 151 579 4.87e-155 452
MS.gene35083.t1 AT5G44410 52.018 446 193 6 1 441 106 535 3.29e-153 446
MS.gene35083.t1 AT1G11770 52.184 435 199 6 1 435 104 529 1.16e-151 442
MS.gene35083.t1 AT1G30700 53.196 438 192 6 1 435 99 526 3.34e-151 441
MS.gene35083.t1 AT1G26420 51.716 437 197 8 1 435 101 525 1.55e-148 434
MS.gene35083.t1 AT4G20840 51.039 433 203 5 3 435 112 535 2.00e-147 432
MS.gene35083.t1 AT1G30710 50.227 440 200 6 1 436 105 529 1.33e-146 429
MS.gene35083.t1 AT1G26380 51.954 435 199 6 1 435 101 525 3.27e-146 428
MS.gene35083.t1 AT1G26390 50.575 435 207 4 1 435 101 527 4.49e-146 428
MS.gene35083.t1 AT1G26410 49.425 435 210 6 1 435 125 549 1.14e-144 425
MS.gene35083.t1 AT1G34575 50.917 436 196 6 1 433 102 522 8.17e-144 422
MS.gene35083.t1 AT1G26400 50.463 432 203 6 1 432 101 521 1.47e-143 421
MS.gene35083.t1 AT1G01980 52.164 439 196 8 2 435 105 534 3.11e-143 421
MS.gene35083.t1 AT1G30730 49.309 434 209 5 1 433 100 523 7.06e-140 412
MS.gene35083.t1 AT4G20830 51.039 433 203 5 3 435 113 536 1.33e-138 409
MS.gene35083.t1 AT4G20800 48.970 437 212 6 1 436 100 526 1.95e-138 408
MS.gene35083.t1 AT4G20830 49.891 459 207 7 3 461 113 548 5.76e-138 409
MS.gene35083.t1 AT1G30740 50.114 437 207 7 1 435 104 531 3.06e-136 403
MS.gene35083.t1 AT1G30740 50.114 437 207 7 1 435 101 528 4.25e-136 402
MS.gene35083.t1 AT1G30720 49.309 434 209 5 1 433 101 524 3.18e-135 400
MS.gene35083.t1 AT2G34810 45.330 439 232 4 1 436 107 540 8.97e-130 387

Find 104 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAAACATTAGGGTCATTT+TGG 0.137007 4.4:-20113140 MS.gene35083:CDS
GCTATAAGCAAGCTAGTATT+TGG 0.193317 4.4:-20112502 MS.gene35083:CDS
AGAGAGCCTATTCCTGATAT+TGG 0.262653 4.4:-20112839 MS.gene35083:CDS
TGACAATATTCAGAGTTTCT+AGG 0.284906 4.4:-20113231 MS.gene35083:CDS
CTGAATATTGTCACAGTTGA+TGG 0.291976 4.4:+20113241 None:intergenic
ACATTTGAATCCTTGTTTCT+TGG 0.300277 4.4:-20113070 MS.gene35083:CDS
ATTCCTGATATTGGGTTAGA+AGG 0.305269 4.4:-20112830 MS.gene35083:CDS
TTGTTTCTTGGAGGTGTAGA+TGG 0.315456 4.4:-20113058 MS.gene35083:CDS
CAATGGGTGAGGATCTGTTT+TGG 0.324567 4.4:-20113333 MS.gene35083:CDS
TGGAATAATGGATGAGATTT+CGG 0.325191 4.4:-20112744 MS.gene35083:CDS
AAGCAAAACTCTTGGTTTCC+CGG 0.331481 4.4:-20113500 MS.gene35083:CDS
ATACCTTCTCTGTCTCGTTT+TGG 0.346393 4.4:-20112389 MS.gene35083:CDS
CACTCCTTATGGTGGAATAA+TGG 0.346906 4.4:-20112756 MS.gene35083:CDS
GATGCGTAAGTTTGGTCTTT+CGG 0.350217 4.4:-20113416 MS.gene35083:CDS
AACCCTTCTAACCCAATATC+AGG 0.364075 4.4:+20112827 None:intergenic
TTGATGAGGACTTAAACATT+AGG 0.365884 4.4:-20113150 MS.gene35083:CDS
CTATAAGCAAGCTAGTATTT+GGG 0.368691 4.4:-20112501 MS.gene35083:CDS
CTTCTCAGTGGCGGCGGCTA+TGG 0.369285 4.4:-20113445 MS.gene35083:CDS
CAAGAATAACTTCAAAAGAT+TGG 0.374379 4.4:-20112465 MS.gene35083:CDS
CTTGGAGACTTTGAAACAAA+AGG 0.375011 4.4:+20112587 None:intergenic
GAGAGCCTATTCCTGATATT+GGG 0.380379 4.4:-20112838 MS.gene35083:CDS
AGATGGACTTATCCCTTTGA+TGG 0.383950 4.4:-20113041 MS.gene35083:CDS
CATCTATTTGGACTTCTTGA+AGG 0.386234 4.4:+20113593 None:intergenic
GGAGGTGGAGGTGCAAGCTT+TGG 0.389215 4.4:-20113301 MS.gene35083:CDS
TTTGAATCCTTGTTTCTTGG+AGG 0.404634 4.4:-20113067 MS.gene35083:CDS
ATATTGGGTTAGAAGGGTTA+TGG 0.406546 4.4:-20112823 MS.gene35083:CDS
TTCCAGAGCTTGGTTTGGTT+AGG 0.411865 4.4:-20113009 MS.gene35083:CDS
TGATGAGGACTTAAACATTA+GGG 0.412260 4.4:-20113149 MS.gene35083:CDS
ATGAGATCAATGACAACAAA+TGG 0.413992 4.4:+20113619 None:intergenic
GTGTTGATATTCACTCCTTA+TGG 0.417599 4.4:-20112767 MS.gene35083:CDS
TCTAACCCAATATCAGGAAT+AGG 0.418514 4.4:+20112833 None:intergenic
GGATTCTTGATGCGTAAGTT+TGG 0.420162 4.4:-20113424 MS.gene35083:CDS
TTCTATTCAACAAAACTTCA+AGG 0.420531 4.4:+20112906 None:intergenic
TAATTCACATATGCAGCTCT+TGG 0.438945 4.4:+20112569 None:intergenic
AATTATAGAGATCTTGATAT+TGG 0.441086 4.4:-20112551 MS.gene35083:CDS
TCCAGAGCTTGGTTTGGTTA+GGG 0.447299 4.4:-20113008 MS.gene35083:CDS
AAAACTCTTGGTTTCCCGGC+AGG 0.448922 4.4:-20113496 MS.gene35083:CDS
AGTTCTTGACAGAGAATCAA+TGG 0.451286 4.4:-20113350 MS.gene35083:CDS
GTTCAAGGAGGGGCAACTGC+TGG 0.456565 4.4:-20113550 MS.gene35083:CDS
TGCCCTACTGTAGGTGTTAG+CGG 0.465359 4.4:-20113469 MS.gene35083:CDS
AATAGCAGTGCATGGGTTCA+AGG 0.466266 4.4:-20113565 MS.gene35083:CDS
TCCCTAACCAAACCAAGCTC+TGG 0.467840 4.4:+20113007 None:intergenic
AATACATTGCCTTGTGAAGA+AGG 0.472389 4.4:-20112347 MS.gene35083:CDS
GGAGTTTCCAGAGCTTGGTT+TGG 0.476037 4.4:-20113014 MS.gene35083:CDS
AAGATTGTACTGAGATGAGT+TGG 0.480382 4.4:-20112985 MS.gene35083:CDS
GAAGAGGAGTTTCCAGAGCT+TGG 0.483846 4.4:-20113019 MS.gene35083:CDS
ATTCAGAGTTTCTAGGACAC+TGG 0.484970 4.4:-20113224 MS.gene35083:CDS
TAGAAGACTTTATAGTTACA+TGG 0.489446 4.4:-20112612 MS.gene35083:CDS
TCATCCATTATTCCACCATA+AGG 0.498834 4.4:+20112752 None:intergenic
TGGGCTATTAGAGGAGGTGG+AGG 0.498949 4.4:-20113313 MS.gene35083:CDS
TTCCTGATATTGGGTTAGAA+GGG 0.500382 4.4:-20112829 MS.gene35083:CDS
TTGGAAAGAGGAAGGTGATG+AGG 0.502301 4.4:-20112660 MS.gene35083:CDS
GGAAACTCCTCTTCCATCAA+AGG 0.513435 4.4:+20113028 None:intergenic
ATTCCATTCCCACACAGAGC+TGG 0.515576 4.4:-20112713 MS.gene35083:CDS
TCTATTCAACAAAACTTCAA+GGG 0.517551 4.4:+20112907 None:intergenic
ACTTATCCCTTTGATGGAAG+AGG 0.525927 4.4:-20113035 MS.gene35083:CDS
TTTCCAGCTCTGTGTGGGAA+TGG 0.526072 4.4:+20112710 None:intergenic
AAATGGAACTTCAGAAACAT+AGG 0.532136 4.4:+20113636 None:intergenic
CTGCCACTTGTGAATAAGCC+TGG 0.540705 4.4:+20113189 None:intergenic
GCCTCGTCTTCATAAAACAT+TGG 0.545940 4.4:+20112800 None:intergenic
CGTAGTCGTGGCCACCACTT+CGG 0.548447 4.4:+20113668 None:intergenic
GAAACTCCTCTTCCATCAAA+GGG 0.558878 4.4:+20113029 None:intergenic
ATGTAGAAAATAGCAGTGCA+TGG 0.560180 4.4:-20113573 MS.gene35083:CDS
AATTGCATCCCTTCTTCACA+AGG 0.562431 4.4:+20112338 None:intergenic
TTGATATTCACTCCTTATGG+TGG 0.565905 4.4:-20112764 MS.gene35083:CDS
ATGCAATTATTAGAGTTCCC+TGG 0.575808 4.4:-20112323 MS.gene35083:CDS
TGACAGAGAATCAATGGGTG+AGG 0.579464 4.4:-20113344 MS.gene35083:CDS
GTTCTTGACAGAGAATCAAT+GGG 0.581403 4.4:-20113349 MS.gene35083:CDS
GCTTTGGAGTTATCATAGCA+TGG 0.581899 4.4:-20113285 MS.gene35083:CDS
GCAGTGCATGGGTTCAAGGA+GGG 0.582300 4.4:-20113561 MS.gene35083:CDS
CAGTAGGGCAAATACCTGCC+GGG 0.584272 4.4:+20113482 None:intergenic
ACAGTAGGGCAAATACCTGC+CGG 0.587491 4.4:+20113481 None:intergenic
TTGTTGAATAGAACTCATAG+TGG 0.587672 4.4:-20112896 MS.gene35083:CDS
CTATTCAACAAAACTTCAAG+GGG 0.590722 4.4:+20112908 None:intergenic
AGTCCGCTAACACCTACAGT+AGG 0.592300 4.4:+20113466 None:intergenic
AGCGGACTTCTCAGTGGCGG+CGG 0.595616 4.4:-20113451 MS.gene35083:CDS
TGTAGAAAATAGCAGTGCAT+GGG 0.602666 4.4:-20113572 MS.gene35083:CDS
ATTGGTGTGAATAATAACAA+TGG 0.605661 4.4:-20112533 MS.gene35083:CDS
ATACATTGCCTTGTGAAGAA+GGG 0.605745 4.4:-20112346 MS.gene35083:CDS
AGGACAGACCTTCGTAGTCG+TGG 0.610475 4.4:+20113656 None:intergenic
GCTCACATAATTGATGTGAA+TGG 0.610798 4.4:-20113376 MS.gene35083:CDS
CAGTGCATGGGTTCAAGGAG+GGG 0.614481 4.4:-20113560 MS.gene35083:CDS
CACTGGAACAAAATGCAACC+AGG 0.614754 4.4:-20113207 MS.gene35083:CDS
GCAGGTATTTGCCCTACTGT+AGG 0.615009 4.4:-20113478 MS.gene35083:CDS
ATCTACACCTCCAAGAAACA+AGG 0.617374 4.4:+20113060 None:intergenic
ATTGAATCAGTTCTCTACCA+TGG 0.621990 4.4:-20112962 MS.gene35083:CDS
AAGAAACAAGGATTCAAATG+TGG 0.623440 4.4:+20113072 None:intergenic
TATAAGCAAGCTAGTATTTG+GGG 0.624423 4.4:-20112500 MS.gene35083:CDS
GTCCGCTAACACCTACAGTA+GGG 0.627164 4.4:+20113467 None:intergenic
CAGATTCGAATCCGAAGTGG+TGG 0.628502 4.4:-20113679 MS.gene35083:CDS
CAACCAGGCTTATTCACAAG+TGG 0.642716 4.4:-20113192 MS.gene35083:CDS
AAATGTTTCCAGCTCTGTGT+GGG 0.643042 4.4:+20112705 None:intergenic
GAATCAGTTCTCTACCATGG+TGG 0.653386 4.4:-20112959 MS.gene35083:CDS
AGTAGCAAATAAGCTTGATG+AGG 0.653614 4.4:-20113164 MS.gene35083:CDS
TTACCAAAACGAGACAGAGA+AGG 0.660634 4.4:+20112386 None:intergenic
AGCAGTGCATGGGTTCAAGG+AGG 0.665984 4.4:-20113562 MS.gene35083:CDS
ATGCAGATTCGAATCCGAAG+TGG 0.674707 4.4:-20113682 MS.gene35083:CDS
TAAATGTTTCCAGCTCTGTG+TGG 0.685122 4.4:+20112704 None:intergenic
TGAATACAAGTACTCAAACA+AGG 0.687986 4.4:-20113111 MS.gene35083:CDS
GTTAGCGGACTTCTCAGTGG+CGG 0.690010 4.4:-20113454 MS.gene35083:CDS
AGTGGTGGCCACGACTACGA+AGG 0.696564 4.4:-20113664 MS.gene35083:CDS
GGTGTTAGCGGACTTCTCAG+TGG 0.701460 4.4:-20113457 MS.gene35083:CDS
TGAAGACGAGGCTAAATCTG+CGG 0.704785 4.4:-20112789 MS.gene35083:CDS
AATCACTTGTAAAACCACCA+TGG 0.721311 4.4:+20112945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAGAGATCTTGATAT+TGG - chr4.4:20113435-20113454 MS.gene35083:CDS 20.0%
!!! TAGAAAAAAGGTACATAAAT+TGG - chr4.4:20113349-20113368 MS.gene35083:CDS 20.0%
! ATTGGTGTGAATAATAACAA+TGG - chr4.4:20113453-20113472 MS.gene35083:CDS 25.0%
! CAAGAATAACTTCAAAAGAT+TGG - chr4.4:20113521-20113540 MS.gene35083:CDS 25.0%
! TAGAAGACTTTATAGTTACA+TGG - chr4.4:20113374-20113393 MS.gene35083:CDS 25.0%
! TCTATTCAACAAAACTTCAA+GGG + chr4.4:20113082-20113101 None:intergenic 25.0%
! TTCTATTCAACAAAACTTCA+AGG + chr4.4:20113083-20113102 None:intergenic 25.0%
!! TCATTTCTGAAAAAGTTTAG+AGG + chr4.4:20113570-20113589 None:intergenic 25.0%
!!! AAAAAACTAAGTGCTGAATT+TGG + chr4.4:20113308-20113327 None:intergenic 25.0%
!!! AAATTCAGCACTTAGTTTTT+TGG - chr4.4:20113307-20113326 MS.gene35083:CDS 25.0%
!!! GTTTTGGTAACATAACAATA+AGG - chr4.4:20113613-20113632 MS.gene35083:CDS 25.0%
!!! TGCTATTTTCTACATCTATT+TGG + chr4.4:20112408-20112427 None:intergenic 25.0%
AAATGGAACTTCAGAAACAT+AGG + chr4.4:20112353-20112372 None:intergenic 30.0%
AAGAAACAAGGATTCAAATG+TGG + chr4.4:20112917-20112936 None:intergenic 30.0%
ATGAGATCAATGACAACAAA+TGG + chr4.4:20112370-20112389 None:intergenic 30.0%
CTATAAGCAAGCTAGTATTT+GGG - chr4.4:20113485-20113504 MS.gene35083:CDS 30.0%
CTATTCAACAAAACTTCAAG+GGG + chr4.4:20113081-20113100 None:intergenic 30.0%
TATAAGCAAGCTAGTATTTG+GGG - chr4.4:20113486-20113505 MS.gene35083:CDS 30.0%
TGAATACAAGTACTCAAACA+AGG - chr4.4:20112875-20112894 MS.gene35083:CDS 30.0%
TGACAATATTCAGAGTTTCT+AGG - chr4.4:20112755-20112774 MS.gene35083:CDS 30.0%
TGATGAGGACTTAAACATTA+GGG - chr4.4:20112837-20112856 MS.gene35083:CDS 30.0%
TTGATGAGGACTTAAACATT+AGG - chr4.4:20112836-20112855 MS.gene35083:CDS 30.0%
TTGTTGAATAGAACTCATAG+TGG - chr4.4:20113090-20113109 MS.gene35083:CDS 30.0%
! ACATTTGAATCCTTGTTTCT+TGG - chr4.4:20112916-20112935 MS.gene35083:CDS 30.0%
! CTTAAACATTAGGGTCATTT+TGG - chr4.4:20112846-20112865 MS.gene35083:CDS 30.0%
!! ATTTTATATGTAGAGCACCA+GGG + chr4.4:20113683-20113702 None:intergenic 30.0%
!! TATTTTATATGTAGAGCACC+AGG + chr4.4:20113684-20113703 None:intergenic 30.0%
!! TGGAATAATGGATGAGATTT+CGG - chr4.4:20113242-20113261 MS.gene35083:CDS 30.0%
!!! AACCAAGAGTTTTGCTTTTT+TGG + chr4.4:20112483-20112502 None:intergenic 30.0%
!!! TTAGTTTTTTGGAAAGAGGA+AGG - chr4.4:20113318-20113337 MS.gene35083:CDS 30.0%
AAGATTGTACTGAGATGAGT+TGG - chr4.4:20113001-20113020 MS.gene35083:CDS 35.0%
AAGGTGATGAGGTAGAAAAA+AGG - chr4.4:20113337-20113356 MS.gene35083:CDS 35.0%
AATACATTGCCTTGTGAAGA+AGG - chr4.4:20113639-20113658 MS.gene35083:CDS 35.0%
AATCACTTGTAAAACCACCA+TGG + chr4.4:20113044-20113063 None:intergenic 35.0%
AGCCAAAAAAGCAAAACTCT+TGG - chr4.4:20112478-20112497 MS.gene35083:CDS 35.0%
AGTAGCAAATAAGCTTGATG+AGG - chr4.4:20112822-20112841 MS.gene35083:CDS 35.0%
AGTTCTTGACAGAGAATCAA+TGG - chr4.4:20112636-20112655 MS.gene35083:CDS 35.0%
ATACATTGCCTTGTGAAGAA+GGG - chr4.4:20113640-20113659 MS.gene35083:CDS 35.0%
ATGCAATTATTAGAGTTCCC+TGG - chr4.4:20113663-20113682 MS.gene35083:CDS 35.0%
ATGTAGAAAATAGCAGTGCA+TGG - chr4.4:20112413-20112432 MS.gene35083:CDS 35.0%
ATTGAATCAGTTCTCTACCA+TGG - chr4.4:20113024-20113043 MS.gene35083:CDS 35.0%
CATCTATTTGGACTTCTTGA+AGG + chr4.4:20112396-20112415 None:intergenic 35.0%
CTGAATATTGTCACAGTTGA+TGG + chr4.4:20112748-20112767 None:intergenic 35.0%
GCTATAAGCAAGCTAGTATT+TGG - chr4.4:20113484-20113503 MS.gene35083:CDS 35.0%
GCTCACATAATTGATGTGAA+TGG - chr4.4:20112610-20112629 MS.gene35083:CDS 35.0%
GTTCTTGACAGAGAATCAAT+GGG - chr4.4:20112637-20112656 MS.gene35083:CDS 35.0%
TAATTCACATATGCAGCTCT+TGG + chr4.4:20113420-20113439 None:intergenic 35.0%
TCATCCATTATTCCACCATA+AGG + chr4.4:20113237-20113256 None:intergenic 35.0%
TCTAACCCAATATCAGGAAT+AGG + chr4.4:20113156-20113175 None:intergenic 35.0%
TGTAGAAAATAGCAGTGCAT+GGG - chr4.4:20112414-20112433 MS.gene35083:CDS 35.0%
TTGATATTCACTCCTTATGG+TGG - chr4.4:20113222-20113241 MS.gene35083:CDS 35.0%
! ATATTGGGTTAGAAGGGTTA+TGG - chr4.4:20113163-20113182 MS.gene35083:CDS 35.0%
! ATTCCTGATATTGGGTTAGA+AGG - chr4.4:20113156-20113175 MS.gene35083:CDS 35.0%
! GTGTTGATATTCACTCCTTA+TGG - chr4.4:20113219-20113238 MS.gene35083:CDS 35.0%
! TTCCTGATATTGGGTTAGAA+GGG - chr4.4:20113157-20113176 MS.gene35083:CDS 35.0%
! TTTGAATCCTTGTTTCTTGG+AGG - chr4.4:20112919-20112938 MS.gene35083:CDS 35.0%
!! ACATTAGGGTCATTTTGGAA+AGG - chr4.4:20112851-20112870 MS.gene35083:CDS 35.0%
!! CATTAGGGTCATTTTGGAAA+GGG - chr4.4:20112852-20112871 MS.gene35083:CDS 35.0%
!! CTTGGAGACTTTGAAACAAA+AGG + chr4.4:20113402-20113421 None:intergenic 35.0%
!!! GCACTTAGTTTTTTGGAAAG+AGG - chr4.4:20113314-20113333 MS.gene35083:CDS 35.0%
!!! TAGTGGTGTGTTGTTTTTCA+AGG - chr4.4:20113107-20113126 MS.gene35083:CDS 35.0%
AAATGTTTCCAGCTCTGTGT+GGG + chr4.4:20113284-20113303 None:intergenic 40.0%
AACCCTTCTAACCCAATATC+AGG + chr4.4:20113162-20113181 None:intergenic 40.0%
AAGCAAAACTCTTGGTTTCC+CGG - chr4.4:20112486-20112505 MS.gene35083:CDS 40.0%
AATTGCATCCCTTCTTCACA+AGG + chr4.4:20113651-20113670 None:intergenic 40.0%
ACTTATCCCTTTGATGGAAG+AGG - chr4.4:20112951-20112970 MS.gene35083:CDS 40.0%
AGAGAGCCTATTCCTGATAT+TGG - chr4.4:20113147-20113166 MS.gene35083:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG + chr4.4:20112929-20112948 None:intergenic 40.0%
GAAACTCCTCTTCCATCAAA+GGG + chr4.4:20112960-20112979 None:intergenic 40.0%
GAGAGCCTATTCCTGATATT+GGG - chr4.4:20113148-20113167 MS.gene35083:CDS 40.0%
GCCTCGTCTTCATAAAACAT+TGG + chr4.4:20113189-20113208 None:intergenic 40.0%
GGATTCTTGATGCGTAAGTT+TGG - chr4.4:20112562-20112581 MS.gene35083:CDS 40.0%
TAAATGTTTCCAGCTCTGTG+TGG + chr4.4:20113285-20113304 None:intergenic 40.0%
TTACCAAAACGAGACAGAGA+AGG + chr4.4:20113603-20113622 None:intergenic 40.0%
! AATGGGTGAGGATCTGTTTT+GGG - chr4.4:20112654-20112673 MS.gene35083:CDS 40.0%
! AGATGGACTTATCCCTTTGA+TGG - chr4.4:20112945-20112964 MS.gene35083:CDS 40.0%
! ATACCTTCTCTGTCTCGTTT+TGG - chr4.4:20113597-20113616 MS.gene35083:CDS 40.0%
! CACTCCTTATGGTGGAATAA+TGG - chr4.4:20113230-20113249 MS.gene35083:CDS 40.0%
! GATGCGTAAGTTTGGTCTTT+CGG - chr4.4:20112570-20112589 MS.gene35083:CDS 40.0%
! GCCAATGTTTTATGAAGACG+AGG - chr4.4:20113185-20113204 MS.gene35083:CDS 40.0%
! TTGTTTCTTGGAGGTGTAGA+TGG - chr4.4:20112928-20112947 MS.gene35083:CDS 40.0%
!! ATTCAGAGTTTCTAGGACAC+TGG - chr4.4:20112762-20112781 MS.gene35083:CDS 40.0%
!! GATCTGTTTTGGGCTATTAG+AGG - chr4.4:20112664-20112683 MS.gene35083:CDS 40.0%
!! GCTTTGGAGTTATCATAGCA+TGG - chr4.4:20112701-20112720 MS.gene35083:CDS 40.0%
AATAGCAGTGCATGGGTTCA+AGG - chr4.4:20112421-20112440 MS.gene35083:CDS 45.0%
ATGCAGATTCGAATCCGAAG+TGG - chr4.4:20112304-20112323 MS.gene35083:CDS 45.0%
CAACCAGGCTTATTCACAAG+TGG - chr4.4:20112794-20112813 MS.gene35083:CDS 45.0%
CACTGGAACAAAATGCAACC+AGG - chr4.4:20112779-20112798 MS.gene35083:CDS 45.0%
GAATCAGTTCTCTACCATGG+TGG - chr4.4:20113027-20113046 MS.gene35083:CDS 45.0%
GGAAACTCCTCTTCCATCAA+AGG + chr4.4:20112961-20112980 None:intergenic 45.0%
TGAAGACGAGGCTAAATCTG+CGG - chr4.4:20113197-20113216 MS.gene35083:CDS 45.0%
TGACAGAGAATCAATGGGTG+AGG - chr4.4:20112642-20112661 MS.gene35083:CDS 45.0%
TTGGAAAGAGGAAGGTGATG+AGG - chr4.4:20113326-20113345 MS.gene35083:CDS 45.0%
! CAATGGGTGAGGATCTGTTT+TGG - chr4.4:20112653-20112672 MS.gene35083:CDS 45.0%
! TTTTGGGCTATTAGAGGAGG+TGG - chr4.4:20112670-20112689 MS.gene35083:CDS 45.0%
!! CTGTTTTGGGCTATTAGAGG+AGG - chr4.4:20112667-20112686 MS.gene35083:CDS 45.0%
!! TCCAGAGCTTGGTTTGGTTA+GGG - chr4.4:20112978-20112997 MS.gene35083:CDS 45.0%
!! TTCCAGAGCTTGGTTTGGTT+AGG - chr4.4:20112977-20112996 MS.gene35083:CDS 45.0%
AAAACTCTTGGTTTCCCGGC+AGG - chr4.4:20112490-20112509 MS.gene35083:CDS 50.0%
ACAGTAGGGCAAATACCTGC+CGG + chr4.4:20112508-20112527 None:intergenic 50.0%
AGTCCGCTAACACCTACAGT+AGG + chr4.4:20112523-20112542 None:intergenic 50.0%
ATTCCATTCCCACACAGAGC+TGG - chr4.4:20113273-20113292 MS.gene35083:CDS 50.0%
CAGATTCGAATCCGAAGTGG+TGG - chr4.4:20112307-20112326 MS.gene35083:CDS 50.0%
GAAGAGGAGTTTCCAGAGCT+TGG - chr4.4:20112967-20112986 MS.gene35083:CDS 50.0%
GCAGGTATTTGCCCTACTGT+AGG - chr4.4:20112508-20112527 MS.gene35083:CDS 50.0%
GGAGTTTCCAGAGCTTGGTT+TGG - chr4.4:20112972-20112991 MS.gene35083:CDS 50.0%
GTCCGCTAACACCTACAGTA+GGG + chr4.4:20112522-20112541 None:intergenic 50.0%
TCCCTAACCAAACCAAGCTC+TGG + chr4.4:20112982-20113001 None:intergenic 50.0%
TGCCCTACTGTAGGTGTTAG+CGG - chr4.4:20112517-20112536 MS.gene35083:CDS 50.0%
TTTCCAGCTCTGTGTGGGAA+TGG + chr4.4:20113279-20113298 None:intergenic 50.0%
! CTGCCACTTGTGAATAAGCC+TGG + chr4.4:20112800-20112819 None:intergenic 50.0%
AGCAGTGCATGGGTTCAAGG+AGG - chr4.4:20112424-20112443 MS.gene35083:CDS 55.0%
AGGACAGACCTTCGTAGTCG+TGG + chr4.4:20112333-20112352 None:intergenic 55.0%
CAGTAGGGCAAATACCTGCC+GGG + chr4.4:20112507-20112526 None:intergenic 55.0%
CAGTGCATGGGTTCAAGGAG+GGG - chr4.4:20112426-20112445 MS.gene35083:CDS 55.0%
GCAGTGCATGGGTTCAAGGA+GGG - chr4.4:20112425-20112444 MS.gene35083:CDS 55.0%
TGGGCTATTAGAGGAGGTGG+AGG - chr4.4:20112673-20112692 MS.gene35083:CDS 55.0%
!! GGTGTTAGCGGACTTCTCAG+TGG - chr4.4:20112529-20112548 MS.gene35083:CDS 55.0%
!! GTTAGCGGACTTCTCAGTGG+CGG - chr4.4:20112532-20112551 MS.gene35083:CDS 55.0%
AGTGGTGGCCACGACTACGA+AGG - chr4.4:20112322-20112341 MS.gene35083:CDS 60.0%
CGTAGTCGTGGCCACCACTT+CGG + chr4.4:20112321-20112340 None:intergenic 60.0%
GTTCAAGGAGGGGCAACTGC+TGG - chr4.4:20112436-20112455 MS.gene35083:CDS 60.0%
! GGAGGTGGAGGTGCAAGCTT+TGG - chr4.4:20112685-20112704 MS.gene35083:CDS 60.0%
CTTCTCAGTGGCGGCGGCTA+TGG - chr4.4:20112541-20112560 MS.gene35083:CDS 65.0%
! AGCGGACTTCTCAGTGGCGG+CGG - chr4.4:20112535-20112554 MS.gene35083:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 20112304 20113704 20112304 ID=MS.gene35083
chr4.4 mRNA 20112304 20113704 20112304 ID=MS.gene35083.t1;Parent=MS.gene35083
chr4.4 exon 20112304 20113704 20112304 ID=MS.gene35083.t1.exon1;Parent=MS.gene35083.t1
chr4.4 CDS 20112304 20113704 20112304 ID=cds.MS.gene35083.t1;Parent=MS.gene35083.t1
Gene Sequence

>MS.gene35083

ATGCAGATTCGAATCCGAAGTGGTGGCCACGACTACGAAGGTCTGTCCTATGTTTCTGAAGTTCCATTTGTTGTCATTGATCTCATAAACCTTCAAGAAGTCCAAATAGATGTAGAAAATAGCAGTGCATGGGTTCAAGGAGGGGCAACTGCTGGCGAACTTTTGTACAAAATTAGCCAAAAAAGCAAAACTCTTGGTTTCCCGGCAGGTATTTGCCCTACTGTAGGTGTTAGCGGACTTCTCAGTGGCGGCGGCTATGGATTCTTGATGCGTAAGTTTGGTCTTTCGGCTGATAATATACTTGACGCTCACATAATTGATGTGAATGGTAGAGTTCTTGACAGAGAATCAATGGGTGAGGATCTGTTTTGGGCTATTAGAGGAGGTGGAGGTGCAAGCTTTGGAGTTATCATAGCATGGAAGATAAAACTAGTTCGTGTTCCATCAACTGTGACAATATTCAGAGTTTCTAGGACACTGGAACAAAATGCAACCAGGCTTATTCACAAGTGGCAGTTAGTAGCAAATAAGCTTGATGAGGACTTAAACATTAGGGTCATTTTGGAAAGGGTGAATACAAGTACTCAAACAAGGAAACTAACAATAAAAGCCACATTTGAATCCTTGTTTCTTGGAGGTGTAGATGGACTTATCCCTTTGATGGAAGAGGAGTTTCCAGAGCTTGGTTTGGTTAGGGAAGATTGTACTGAGATGAGTTGGATTGAATCAGTTCTCTACCATGGTGGTTTTACAAGTGATTTTACAAGTGATCAACCCCTTGAAGTTTTGTTGAATAGAACTCATAGTGGTGTGTTGTTTTTCAAGGCAAAATCTGATTATGTGAGAGAGCCTATTCCTGATATTGGGTTAGAAGGGTTATGGCCAATGTTTTATGAAGACGAGGCTAAATCTGCGGTGTTGATATTCACTCCTTATGGTGGAATAATGGATGAGATTTCGGAGTCTGAAATTCCATTCCCACACAGAGCTGGAAACATTTACCAAATTCAGCACTTAGTTTTTTGGAAAGAGGAAGGTGATGAGGTAGAAAAAAGGTACATAAATTGGATTAGAAGACTTTATAGTTACATGGAACCTTTTGTTTCAAAGTCTCCAAGAGCTGCATATGTGAATTATAGAGATCTTGATATTGGTGTGAATAATAACAATGGATACACAAGCTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTCAAGAATAACTTCAAAAGATTGGCAAAAGTGAAGACTAAAGTTGATCCTCTAAACTTTTTCAGAAATGAACAGAGTATACCTTCTCTGTCTCGTTTTGGTAACATAACAATAAGGCATAATACATTGCCTTGTGAAGAAGGGATGCAATTATTAGAGTTCCCTGGTGCTCTACATATAAAATAA
Protein sequence

>MS.gene35083.t1

MQIRIRSGGHDYEGLSYVSEVPFVVIDLINLQEVQIDVENSSAWVQGGATAGELLYKISQKSKTLGFPAGICPTVGVSGLLSGGGYGFLMRKFGLSADNILDAHIIDVNGRVLDRESMGEDLFWAIRGGGGASFGVIIAWKIKLVRVPSTVTIFRVSRTLEQNATRLIHKWQLVANKLDEDLNIRVILERVNTSTQTRKLTIKATFESLFLGGVDGLIPLMEEEFPELGLVREDCTEMSWIESVLYHGGFTSDFTSDQPLEVLLNRTHSGVLFFKAKSDYVREPIPDIGLEGLWPMFYEDEAKSAVLIFTPYGGIMDEISESEIPFPHRAGNIYQIQHLVFWKEEGDEVEKRYINWIRRLYSYMEPFVSKSPRAAYVNYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSLSRFGNITIRHNTLPCEEGMQLLEFPGALHIK