AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028347

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028347.t1 MTR_4g094518 98.120 532 10 0 1 532 1 532 0.0 1079
MS.gene028347.t1 MTR_2g031390 80.354 509 100 0 24 532 23 531 0.0 867
MS.gene028347.t1 MTR_4g094520 70.152 526 147 3 10 532 16 534 0.0 735
MS.gene028347.t1 MTR_3g009760 53.890 527 229 9 10 530 4 522 0.0 556
MS.gene028347.t1 MTR_2g031590 51.493 536 249 7 2 531 3 533 0.0 555
MS.gene028347.t1 MTR_3g009820 53.700 527 230 9 10 530 4 522 0.0 555
MS.gene028347.t1 MTR_4g094488 53.672 531 235 6 6 528 8 535 0.0 553
MS.gene028347.t1 MTR_3g009880 53.333 525 233 8 10 530 4 520 0.0 552
MS.gene028347.t1 MTR_2g031560 50.476 525 254 5 8 530 3 523 0.0 551
MS.gene028347.t1 MTR_4g094498 55.110 499 218 3 33 530 41 534 0.0 551
MS.gene028347.t1 MTR_4g094492 52.045 538 244 7 2 530 4 536 0.0 545
MS.gene028347.t1 MTR_4g094495 51.977 531 246 5 5 528 10 538 0.0 533
MS.gene028347.t1 MTR_2g031460 52.579 504 234 4 27 530 29 527 0.0 532
MS.gene028347.t1 MTR_4g094478 51.803 527 245 6 10 530 12 535 0.0 531
MS.gene028347.t1 MTR_2g031420 51.429 525 250 4 6 530 8 527 0.0 531
MS.gene028347.t1 MTR_4g094480 50.187 534 254 7 4 528 3 533 2.10e-180 518
MS.gene028347.t1 MTR_4g094418 55.378 437 185 5 102 530 1 435 5.86e-174 504
MS.gene028347.t1 MTR_2g031490 49.057 530 260 6 8 531 14 539 4.87e-169 489
MS.gene028347.t1 MTR_4g091200 49.588 486 231 7 50 528 46 524 8.18e-167 483
MS.gene028347.t1 MTR_2g031510 49.116 509 252 4 27 531 36 541 4.98e-165 479
MS.gene028347.t1 MTR_6g017205 47.180 532 253 10 11 528 7 524 1.79e-164 478
MS.gene028347.t1 MTR_4g091150 48.337 511 248 9 29 530 37 540 6.90e-164 476
MS.gene028347.t1 MTR_4g094515 47.368 532 273 5 4 531 623 1151 7.03e-164 495
MS.gene028347.t1 MTR_4g094515 48.617 506 253 4 30 531 39 541 9.73e-156 474
MS.gene028347.t1 MTR_4g088355 47.421 504 256 7 30 528 32 531 1.58e-162 473
MS.gene028347.t1 MTR_4g057990 46.881 529 258 10 8 528 7 520 1.61e-159 464
MS.gene028347.t1 MTR_4g091190 45.747 529 267 8 11 528 7 526 7.54e-159 463
MS.gene028347.t1 MTR_4g091170 45.614 456 226 6 75 528 105 540 7.35e-149 439
MS.gene028347.t1 MTR_2g023590 46.352 466 240 6 67 528 5 464 4.31e-147 431
MS.gene028347.t1 MTR_1g076830 40.079 509 293 6 30 530 31 535 2.58e-118 360
MS.gene028347.t1 MTR_3g009850 56.579 228 97 2 305 530 1 228 1.51e-85 264
MS.gene028347.t1 MTR_2g031600 52.846 246 108 3 1 246 7 244 1.32e-78 252
MS.gene028347.t1 MTR_2g031600 54.140 157 71 1 376 531 245 401 1.64e-56 194
MS.gene028347.t1 MTR_8g062280 57.732 97 13 1 431 527 4 72 3.51e-32 118
MS.gene028347.t1 MTR_2g084370 43.243 111 41 2 128 238 1 89 4.09e-26 102
MS.gene028347.t1 MTR_2g084405 43.478 92 47 1 128 219 1 87 2.84e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028347.t1 AT1G30760 65.873 504 166 2 27 528 49 548 0.0 686
MS.gene028347.t1 AT1G30760 65.873 504 166 2 27 528 31 530 0.0 685
MS.gene028347.t1 AT2G34790 63.289 523 184 3 8 528 12 528 0.0 674
MS.gene028347.t1 AT5G44400 62.384 537 191 7 2 530 3 536 0.0 660
MS.gene028347.t1 AT5G44390 58.704 540 206 8 7 532 6 542 0.0 619
MS.gene028347.t1 AT5G44380 59.728 514 199 5 23 531 70 580 0.0 592
MS.gene028347.t1 AT5G44380 59.728 514 199 5 23 531 30 540 0.0 592
MS.gene028347.t1 AT1G30700 51.881 505 238 4 29 531 26 527 1.19e-176 508
MS.gene028347.t1 AT4G20820 50.198 506 236 8 28 530 34 526 2.60e-170 492
MS.gene028347.t1 AT5G44360 48.399 531 256 10 10 532 12 532 1.65e-167 485
MS.gene028347.t1 AT5G44360 48.399 531 256 10 10 532 28 548 2.09e-167 486
MS.gene028347.t1 AT4G20840 48.723 509 245 7 27 528 34 533 5.04e-164 476
MS.gene028347.t1 AT4G20860 51.974 456 211 6 77 530 80 529 4.76e-163 474
MS.gene028347.t1 AT1G34575 46.943 507 252 8 27 528 28 522 4.71e-162 471
MS.gene028347.t1 AT1G30710 47.081 531 262 9 8 530 9 528 1.94e-161 469
MS.gene028347.t1 AT1G11770 46.756 524 267 8 13 531 14 530 1.27e-159 465
MS.gene028347.t1 AT5G44440 47.843 510 247 9 28 528 28 527 1.04e-158 463
MS.gene028347.t1 AT5G44440 46.765 541 262 11 4 528 67 597 1.32e-158 465
MS.gene028347.t1 AT4G20800 46.614 502 263 4 29 529 27 524 1.07e-157 460
MS.gene028347.t1 AT1G26380 47.048 525 264 9 11 530 10 525 1.45e-157 460
MS.gene028347.t1 AT5G44410 49.903 513 226 11 28 528 34 527 1.19e-156 457
MS.gene028347.t1 AT1G26420 46.023 528 277 7 8 532 5 527 2.97e-154 451
MS.gene028347.t1 AT1G30730 45.992 524 275 6 8 528 5 523 1.45e-153 449
MS.gene028347.t1 AT1G30720 48.111 503 251 7 30 528 28 524 4.23e-152 446
MS.gene028347.t1 AT1G26390 46.535 505 263 5 31 532 29 529 7.61e-152 445
MS.gene028347.t1 AT4G20830 47.937 509 249 7 29 530 37 536 3.05e-151 444
MS.gene028347.t1 AT4G20830 47.937 509 249 7 29 530 37 536 1.54e-150 443
MS.gene028347.t1 AT1G26410 45.743 505 265 7 31 532 53 551 6.23e-149 438
MS.gene028347.t1 AT1G26400 46.463 523 267 8 11 529 10 523 6.29e-149 437
MS.gene028347.t1 AT1G01980 46.304 514 261 8 27 531 28 535 1.90e-146 432
MS.gene028347.t1 AT1G30740 45.216 533 277 9 8 531 3 529 1.05e-145 429
MS.gene028347.t1 AT1G30740 45.216 533 277 9 8 531 6 532 1.23e-145 429
MS.gene028347.t1 AT2G34810 45.349 516 269 8 24 530 28 539 2.47e-142 421

Find 115 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGAAAGGTGTTTCGATTT+TGG 0.085027 4.2:+17957559 None:intergenic
CCACCTGAAGTTCTTCTTAA+AGG 0.167526 4.2:-17956846 MS.gene028347:CDS
CCATGGGAGAGGATCTATTT+TGG 0.198723 4.2:-17957259 MS.gene028347:CDS
GAAAACAAGTTTCCCTGAAT+TGG 0.232841 4.2:-17956946 MS.gene028347:CDS
GAAGCTTTGGTATACTTCTT+TGG 0.234046 4.2:-17957214 MS.gene028347:CDS
TTCCTTGTCAAACCCAATTC+AGG 0.239581 4.2:+17956933 None:intergenic
TACGAGAAAAGTTCGGTTCT+TGG 0.245296 4.2:-17957434 MS.gene028347:CDS
AAGCGACTGGTTGGGGTTAT+AGG 0.248483 4.2:-17956443 MS.gene028347:CDS
AAGATAGCCCTTTGATGATT+TGG 0.263280 4.2:-17956722 MS.gene028347:CDS
GGGAAGCTTATGTGAATTAT+AGG 0.274126 4.2:-17956512 MS.gene028347:CDS
GAATTATAGGGATCTTGATT+TGG 0.282140 4.2:-17956499 MS.gene028347:CDS
GATAATAGAAGGACTAAAAT+TGG 0.290188 4.2:+17957893 None:intergenic
AATTATAGGGATCTTGATTT+GGG 0.302116 4.2:-17956498 MS.gene028347:CDS
AATCCTTCAATACCAGTTTC+TGG 0.308973 4.2:+17956765 None:intergenic
AAATTCAGTACTTGTCTATA+TGG 0.320076 4.2:-17956623 MS.gene028347:CDS
CTGCTTGGACATGGGAATCT+TGG 0.323117 4.2:+17957663 None:intergenic
TCAATACCAGTTTCTGGTAT+TGG 0.333889 4.2:+17956771 None:intergenic
GTTGAGAATGAAGGTTCATT+TGG 0.337178 4.2:+17957764 None:intergenic
CAAAGCATGTAGACTGGATT+AGG 0.340081 4.2:-17956578 MS.gene028347:CDS
GAGGGTGTGAATATGAATTC+AGG 0.357365 4.2:+17957686 None:intergenic
ACTGTCACTGTTGGTGGAAC+AGG 0.360366 4.2:+17957173 None:intergenic
AAAACAAGTTTCCCTGAATT+GGG 0.364442 4.2:-17956945 MS.gene028347:CDS
GTTCCATTTCTATGTGGAAA+AGG 0.370940 4.2:+17956654 None:intergenic
GGAACATTGATGAGAAAATA+TGG 0.375969 4.2:-17957350 MS.gene028347:CDS
AAATCTGTTCTCTATATTGC+TGG 0.377366 4.2:-17956885 MS.gene028347:CDS
CTTGGAAACTTTGACACATA+AGG 0.377974 4.2:+17956534 None:intergenic
TGCTTGGACATGGGAATCTT+GGG 0.378635 4.2:+17957664 None:intergenic
AATTCAGGTTTCGGTGTAGA+TGG 0.385209 4.2:+17957701 None:intergenic
CTATATAGAAGCGACTGGTT+GGG 0.401524 4.2:-17956451 MS.gene028347:CDS
GTGTAAATTGTTGTATGAAA+TGG 0.405051 4.2:+17957788 None:intergenic
GGTGGTGGAGGTGGAAGCTT+TGG 0.413015 4.2:-17957227 MS.gene028347:CDS
TTTCCTTTAAGAAGAACTTC+AGG 0.413371 4.2:+17956843 None:intergenic
ATGAAAGTGATGATAATAGA+AGG 0.416106 4.2:+17957882 None:intergenic
AGCAATGAACCTAAGGTGTT+TGG 0.416350 4.2:-17957726 MS.gene028347:CDS
GTGAATATGAATTCAGGTTT+CGG 0.420392 4.2:+17957692 None:intergenic
TCGGTTCTTGGTTACCCTGC+AGG 0.425653 4.2:-17957422 MS.gene028347:CDS
CACATAACAGGAGGTGCATA+TGG 0.426699 4.2:-17957371 MS.gene028347:CDS
GCTATAAGAGGTGGTGGAGG+TGG 0.427869 4.2:-17957236 MS.gene028347:CDS
CTGAATTCAGGTTGAGACAT+TGG 0.430119 4.2:+17957822 None:intergenic
GGAAGCTTATGTGAATTATA+GGG 0.435715 4.2:-17956511 MS.gene028347:CDS
ACACTAAAACCAACACTGCT+TGG 0.438629 4.2:-17957499 MS.gene028347:CDS
AAAATTGGCAAGGGTAAGAT+TGG 0.445220 4.2:+17957908 None:intergenic
GCTCGAATCGTTGATGCAAA+TGG 0.448524 4.2:-17957302 MS.gene028347:CDS
TTCCATTTCTATGTGGAAAA+GGG 0.450370 4.2:+17956655 None:intergenic
CACTAAAACCAACACTGCTT+GGG 0.452600 4.2:-17957498 MS.gene028347:CDS
TCCTTGTCAAACCCAATTCA+GGG 0.453047 4.2:+17956934 None:intergenic
TATATAGAAGCGACTGGTTG+GGG 0.455293 4.2:-17956450 MS.gene028347:CDS
GCTATATAGAAGCGACTGGT+TGG 0.459836 4.2:-17956452 MS.gene028347:CDS
GGTGTCGGTGGACACATAAC+AGG 0.463630 4.2:-17957383 MS.gene028347:CDS
TGGATCCTGAAAATGTGTTT+AGG 0.464198 4.2:-17956374 MS.gene028347:CDS
AAGATTGTCAAGAAACTAGT+TGG 0.464462 4.2:-17956911 MS.gene028347:CDS
AGGGAACCAATACCAGAAAC+TGG 0.473053 4.2:-17956777 MS.gene028347:CDS
AAGCTTGTGCATAGACCTGC+AGG 0.473568 4.2:+17957407 None:intergenic
GCAGGTCTATGCACAAGCTT+AGG 0.479193 4.2:-17957404 MS.gene028347:CDS
TTGATGATTTGGAATCCATA+TGG 0.487109 4.2:-17956711 MS.gene028347:CDS
GGTTCATTGCTAAGGAATCA+AGG 0.487996 4.2:+17957738 None:intergenic
AAATTACGGCTGCTTGGACA+TGG 0.488116 4.2:+17957654 None:intergenic
GTAATAGACTTCACCGATAG+TGG 0.498495 4.2:+17957463 None:intergenic
TAATTCACATAAGCTTCCCT+TGG 0.500621 4.2:+17956516 None:intergenic
TTGGACATGGGAATCTTGGG+AGG 0.501683 4.2:+17957667 None:intergenic
CAGAATATACGAGAAAAGTT+CGG 0.501978 4.2:-17957441 MS.gene028347:CDS
AACACTGCTTGGGTTCAATC+CGG 0.504753 4.2:-17957488 MS.gene028347:CDS
ATTCCTTAGCAATGAACCTA+AGG 0.505108 4.2:-17957733 MS.gene028347:CDS
TTGAAAGAATTCTTGAATGT+TGG 0.506211 4.2:+17956819 None:intergenic
TGGAGCAAATTACGGCTGCT+TGG 0.506670 4.2:+17957648 None:intergenic
ATACCAGAAACTGGTATTGA+AGG 0.507113 4.2:-17956768 MS.gene028347:CDS
ACACCTTAGGTTCATTGCTA+AGG 0.508803 4.2:+17957730 None:intergenic
TAGATGGTGCCAAACACCTT+AGG 0.511494 4.2:+17957717 None:intergenic
CTTATGTGTCAAAGTTTCCA+AGG 0.512905 4.2:-17956533 MS.gene028347:CDS
TACAATGTTCCATTTCTATG+TGG 0.513684 4.2:+17956648 None:intergenic
TTGTTTCCAACACTAGCAGC+TGG 0.514986 4.2:+17957041 None:intergenic
TGGGCTATAAGAGGTGGTGG+AGG 0.525036 4.2:-17957239 MS.gene028347:CDS
ACTGTCTTGAAGTAATAAAG+AGG 0.526763 4.2:+17957850 None:intergenic
ATCCAACCAGCTGCTAGTGT+TGG 0.528310 4.2:-17957047 MS.gene028347:CDS
GTCGATTTAGTCAAACTTCG+CGG 0.531435 4.2:-17957533 MS.gene028347:CDS
TATGGATTCCAAATCATCAA+AGG 0.532792 4.2:+17956714 None:intergenic
GTTCAATCCGGTGCCACTAT+CGG 0.538120 4.2:-17957476 MS.gene028347:CDS
TTGATGAGAAAATATGGTCT+TGG 0.539868 4.2:-17957344 MS.gene028347:CDS
AATTACGGCTGCTTGGACAT+GGG 0.540054 4.2:+17957655 None:intergenic
ATGGATTCCAAATCATCAAA+GGG 0.540969 4.2:+17956715 None:intergenic
GCTTTGGTATACTTCTTTGG+TGG 0.541590 4.2:-17957211 MS.gene028347:CDS
TCAAGGATGGTTGAGAATGA+AGG 0.541625 4.2:+17957755 None:intergenic
TTCCAACACTAGCAGCTGGT+TGG 0.544033 4.2:+17957045 None:intergenic
CATTCTCGACAGAAAAGCCA+TGG 0.561462 4.2:-17957276 MS.gene028347:CDS
ATGAAAGTCCTTCATAGTCA+TGG 0.565899 4.2:+17957588 None:intergenic
GACTTCACCGATAGTGGCAC+CGG 0.566405 4.2:+17957469 None:intergenic
CGACAGAAAAGCCATGGGAG+AGG 0.567021 4.2:-17957270 MS.gene028347:CDS
TAGAAGGACTAAAATTGGCA+AGG 0.572624 4.2:+17957898 None:intergenic
GGATTGAACCCAAGCAGTGT+TGG 0.573765 4.2:+17957490 None:intergenic
AGAAGGACTAAAATTGGCAA+GGG 0.574034 4.2:+17957899 None:intergenic
GTATTGAAGGATTGTGGCAG+AGG 0.576392 4.2:-17956755 MS.gene028347:CDS
ACTTCAGGTGGTGTAACACT+TGG 0.576670 4.2:+17956858 None:intergenic
ATGTTGCAAAGCATGTAGAC+TGG 0.576679 4.2:-17956584 MS.gene028347:CDS
CATTGCTAAGGAATCAAGGA+TGG 0.582931 4.2:+17957742 None:intergenic
AGTGGTGGCCATGACTATGA+AGG 0.587071 4.2:-17957596 MS.gene028347:CDS
ACTAAATCGACAACTATGAA+AGG 0.587233 4.2:+17957545 None:intergenic
TCCCTGAATTGGGTTTGACA+AGG 0.596787 4.2:-17956935 MS.gene028347:CDS
TTATGTGTCAAAGTTTCCAA+GGG 0.597414 4.2:-17956532 MS.gene028347:CDS
CCTTTAAGAAGAACTTCAGG+TGG 0.600002 4.2:+17956846 None:intergenic
GTACTTGTCTATATGGCAAG+AGG 0.601770 4.2:-17956616 MS.gene028347:CDS
CATCTAAGAGTAAGAAGTGG+TGG 0.603654 4.2:-17957611 MS.gene028347:CDS
ACAAGCTATATAGAAGCGAC+TGG 0.606255 4.2:-17956456 MS.gene028347:CDS
CCAAAATAGATCCTCTCCCA+TGG 0.610823 4.2:+17957259 None:intergenic
ATGATTTGGAATCCATATGG+TGG 0.611221 4.2:-17956708 MS.gene028347:CDS
AAACTGGTATTGAAGGATTG+TGG 0.613890 4.2:-17956761 MS.gene028347:CDS
TGGACATGGGAATCTTGGGA+GGG 0.614117 4.2:+17957668 None:intergenic
CTATGCACAAGCTTAGGTGT+CGG 0.620969 4.2:-17957398 MS.gene028347:CDS
GGTTCAGATAAAGACTAGAG+TGG 0.623449 4.2:-17956394 MS.gene028347:CDS
TACTTGTCTATATGGCAAGA+GGG 0.628128 4.2:-17956615 MS.gene028347:CDS
TGCACAAGCTTAGGTGTCGG+TGG 0.631461 4.2:-17957395 MS.gene028347:CDS
ATTCATCTAAGAGTAAGAAG+TGG 0.659014 4.2:-17957614 MS.gene028347:CDS
GTCACTAAAACTCTAGAACA+AGG 0.665135 4.2:-17957143 MS.gene028347:CDS
ATTCTCGACAGAAAAGCCAT+GGG 0.672433 4.2:-17957275 MS.gene028347:CDS
GTCGGTGGACACATAACAGG+AGG 0.682458 4.2:-17957380 MS.gene028347:CDS
AGCTTGTGCATAGACCTGCA+GGG 0.712907 4.2:+17957408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAGATTTTCATCAATAAA+AGG + chr4.2:17957178-17957197 None:intergenic 20.0%
! AAATTCAGTACTTGTCTATA+TGG - chr4.2:17957635-17957654 MS.gene028347:CDS 25.0%
! AATTATAGGGATCTTGATTT+GGG - chr4.2:17957760-17957779 MS.gene028347:CDS 25.0%
! ATGAAAGTGATGATAATAGA+AGG + chr4.2:17956379-17956398 None:intergenic 25.0%
! CTACAAAAATTCTTCATAGA+TGG - chr4.2:17957140-17957159 MS.gene028347:CDS 25.0%
! GATAATAGAAGGACTAAAAT+TGG + chr4.2:17956368-17956387 None:intergenic 25.0%
! GTGTAAATTGTTGTATGAAA+TGG + chr4.2:17956473-17956492 None:intergenic 25.0%
! TTGAAAGAATTCTTGAATGT+TGG + chr4.2:17957442-17957461 None:intergenic 25.0%
!!! AAGTTTTTTGGAGCAAATTA+CGG + chr4.2:17956621-17956640 None:intergenic 25.0%
!!! TAAGGGTAATTTTAACAAGT+TGG - chr4.2:17957843-17957862 MS.gene028347:CDS 25.0%
!!! TAGATGAATTCCAAGTTTTT+TGG + chr4.2:17956633-17956652 None:intergenic 25.0%
AAAACAAGTTTCCCTGAATT+GGG - chr4.2:17957313-17957332 MS.gene028347:CDS 30.0%
AAATCTGTTCTCTATATTGC+TGG - chr4.2:17957373-17957392 MS.gene028347:CDS 30.0%
AAGATTGTCAAGAAACTAGT+TGG - chr4.2:17957347-17957366 MS.gene028347:CDS 30.0%
ACTAAATCGACAACTATGAA+AGG + chr4.2:17956716-17956735 None:intergenic 30.0%
ACTGTCTTGAAGTAATAAAG+AGG + chr4.2:17956411-17956430 None:intergenic 30.0%
ATGGATTCCAAATCATCAAA+GGG + chr4.2:17957546-17957565 None:intergenic 30.0%
ATTCATCTAAGAGTAAGAAG+TGG - chr4.2:17956644-17956663 MS.gene028347:CDS 30.0%
CAGAATATACGAGAAAAGTT+CGG - chr4.2:17956817-17956836 MS.gene028347:CDS 30.0%
GAATTATAGGGATCTTGATT+TGG - chr4.2:17957759-17957778 MS.gene028347:CDS 30.0%
GGAACATTGATGAGAAAATA+TGG - chr4.2:17956908-17956927 MS.gene028347:CDS 30.0%
GGAAGCTTATGTGAATTATA+GGG - chr4.2:17957747-17957766 MS.gene028347:CDS 30.0%
TACAATGTTCCATTTCTATG+TGG + chr4.2:17957613-17957632 None:intergenic 30.0%
TATGGATTCCAAATCATCAA+AGG + chr4.2:17957547-17957566 None:intergenic 30.0%
TTATGTGTCAAAGTTTCCAA+GGG - chr4.2:17957726-17957745 MS.gene028347:CDS 30.0%
TTCCATTTCTATGTGGAAAA+GGG + chr4.2:17957606-17957625 None:intergenic 30.0%
TTGATGAGAAAATATGGTCT+TGG - chr4.2:17956914-17956933 MS.gene028347:CDS 30.0%
! AAGCAAAATCAGATTTTGTG+AGG - chr4.2:17957461-17957480 MS.gene028347:CDS 30.0%
! AGCAAAATCAGATTTTGTGA+GGG - chr4.2:17957462-17957481 MS.gene028347:CDS 30.0%
! GATATGTTTTGTCTGAATTC+AGG + chr4.2:17956451-17956470 None:intergenic 30.0%
! GTGAATATGAATTCAGGTTT+CGG + chr4.2:17956569-17956588 None:intergenic 30.0%
! TTGATGATTTGGAATCCATA+TGG - chr4.2:17957547-17957566 MS.gene028347:CDS 30.0%
! TTGGGGTTATAGGTATTTTA+AGG - chr4.2:17957825-17957844 MS.gene028347:CDS 30.0%
! TTTCCTTTAAGAAGAACTTC+AGG + chr4.2:17957418-17957437 None:intergenic 30.0%
!! GTAATTTGCTCCAAAAAACT+TGG - chr4.2:17956620-17956639 MS.gene028347:CDS 30.0%
!! TGGGGTTATAGGTATTTTAA+GGG - chr4.2:17957826-17957845 MS.gene028347:CDS 30.0%
!!! TATGAAAGGTGTTTCGATTT+TGG + chr4.2:17956702-17956721 None:intergenic 30.0%
!!! TCTTACAATGCTGTTTTTCT+TGG - chr4.2:17957262-17957281 MS.gene028347:CDS 30.0%
AAAATTGGCAAGGGTAAGAT+TGG + chr4.2:17956353-17956372 None:intergenic 35.0%
AATCCTTCAATACCAGTTTC+TGG + chr4.2:17957496-17957515 None:intergenic 35.0%
ACAGTAAAAACTGTCACTGT+TGG + chr4.2:17957097-17957116 None:intergenic 35.0%
AGAAGGACTAAAATTGGCAA+GGG + chr4.2:17956362-17956381 None:intergenic 35.0%
ATGAAAGTCCTTCATAGTCA+TGG + chr4.2:17956673-17956692 None:intergenic 35.0%
ATTCCTTAGCAATGAACCTA+AGG - chr4.2:17956525-17956544 MS.gene028347:CDS 35.0%
CTTATGTGTCAAAGTTTCCA+AGG - chr4.2:17957725-17957744 MS.gene028347:CDS 35.0%
CTTGGAAACTTTGACACATA+AGG + chr4.2:17957727-17957746 None:intergenic 35.0%
GAAAACAAGTTTCCCTGAAT+TGG - chr4.2:17957312-17957331 MS.gene028347:CDS 35.0%
GGGAAGCTTATGTGAATTAT+AGG - chr4.2:17957746-17957765 MS.gene028347:CDS 35.0%
GTTCCATTTCTATGTGGAAA+AGG + chr4.2:17957607-17957626 None:intergenic 35.0%
GTTGAGAATGAAGGTTCATT+TGG + chr4.2:17956497-17956516 None:intergenic 35.0%
TAATTCACATAAGCTTCCCT+TGG + chr4.2:17957745-17957764 None:intergenic 35.0%
TACTTGTCTATATGGCAAGA+GGG - chr4.2:17957643-17957662 MS.gene028347:CDS 35.0%
TAGAAGGACTAAAATTGGCA+AGG + chr4.2:17956363-17956382 None:intergenic 35.0%
TGGATCCTGAAAATGTGTTT+AGG - chr4.2:17957884-17957903 MS.gene028347:CDS 35.0%
! AAACTGGTATTGAAGGATTG+TGG - chr4.2:17957497-17957516 MS.gene028347:CDS 35.0%
! AAGATAGCCCTTTGATGATT+TGG - chr4.2:17957536-17957555 MS.gene028347:CDS 35.0%
! ATACCAGAAACTGGTATTGA+AGG - chr4.2:17957490-17957509 MS.gene028347:CDS 35.0%
! ATGATTTGGAATCCATATGG+TGG - chr4.2:17957550-17957569 MS.gene028347:CDS 35.0%
! GAAGCTTTGGTATACTTCTT+TGG - chr4.2:17957044-17957063 MS.gene028347:CDS 35.0%
! TCATGCCTAAACACATTTTC+AGG + chr4.2:17957892-17957911 None:intergenic 35.0%
!! GATCTATTTTGGGCTATAAG+AGG - chr4.2:17957010-17957029 MS.gene028347:CDS 35.0%
!! GTCACTAAAACTCTAGAACA+AGG - chr4.2:17957115-17957134 MS.gene028347:CDS 35.0%
!! TCAATACCAGTTTCTGGTAT+TGG + chr4.2:17957490-17957509 None:intergenic 35.0%
!! TCACTCATTTTTCCACCATA+TGG + chr4.2:17957565-17957584 None:intergenic 35.0%
ACAAGCTATATAGAAGCGAC+TGG - chr4.2:17957802-17957821 MS.gene028347:CDS 40.0%
ACACCTTAGGTTCATTGCTA+AGG + chr4.2:17956531-17956550 None:intergenic 40.0%
ACACTAAAACCAACACTGCT+TGG - chr4.2:17956759-17956778 MS.gene028347:CDS 40.0%
ATGTTGCAAAGCATGTAGAC+TGG - chr4.2:17957674-17957693 MS.gene028347:CDS 40.0%
ATTCTCGACAGAAAAGCCAT+GGG - chr4.2:17956983-17957002 MS.gene028347:CDS 40.0%
CAAAGCATGTAGACTGGATT+AGG - chr4.2:17957680-17957699 MS.gene028347:CDS 40.0%
CACTAAAACCAACACTGCTT+GGG - chr4.2:17956760-17956779 MS.gene028347:CDS 40.0%
CATCTAAGAGTAAGAAGTGG+TGG - chr4.2:17956647-17956666 MS.gene028347:CDS 40.0%
CATTGCTAAGGAATCAAGGA+TGG + chr4.2:17956519-17956538 None:intergenic 40.0%
CCACCTGAAGTTCTTCTTAA+AGG - chr4.2:17957412-17957431 MS.gene028347:CDS 40.0%
GCTTTGGTATACTTCTTTGG+TGG - chr4.2:17957047-17957066 MS.gene028347:CDS 40.0%
GGTTCAGATAAAGACTAGAG+TGG - chr4.2:17957864-17957883 MS.gene028347:CDS 40.0%
GGTTCATTGCTAAGGAATCA+AGG + chr4.2:17956523-17956542 None:intergenic 40.0%
GTAAAAACTGTCACTGTTGG+TGG + chr4.2:17957094-17957113 None:intergenic 40.0%
GTAATAGACTTCACCGATAG+TGG + chr4.2:17956798-17956817 None:intergenic 40.0%
GTACTTGTCTATATGGCAAG+AGG - chr4.2:17957642-17957661 MS.gene028347:CDS 40.0%
TACGAGAAAAGTTCGGTTCT+TGG - chr4.2:17956824-17956843 MS.gene028347:CDS 40.0%
TCAAGGATGGTTGAGAATGA+AGG + chr4.2:17956506-17956525 None:intergenic 40.0%
TCCTTGTCAAACCCAATTCA+GGG + chr4.2:17957327-17957346 None:intergenic 40.0%
TTCCTTGTCAAACCCAATTC+AGG + chr4.2:17957328-17957347 None:intergenic 40.0%
! AATTCAGGTTTCGGTGTAGA+TGG + chr4.2:17956560-17956579 None:intergenic 40.0%
! AGCAATGAACCTAAGGTGTT+TGG - chr4.2:17956532-17956551 MS.gene028347:CDS 40.0%
! AGCCCTTTTCCACATAGAAA+TGG - chr4.2:17957601-17957620 MS.gene028347:CDS 40.0%
! CATGGGAGAGGATCTATTTT+GGG - chr4.2:17957000-17957019 MS.gene028347:CDS 40.0%
! CCTTTAAGAAGAACTTCAGG+TGG + chr4.2:17957415-17957434 None:intergenic 40.0%
! CTATATAGAAGCGACTGGTT+GGG - chr4.2:17957807-17957826 MS.gene028347:CDS 40.0%
! GAGGGTGTGAATATGAATTC+AGG + chr4.2:17956575-17956594 None:intergenic 40.0%
! GTCGATTTAGTCAAACTTCG+CGG - chr4.2:17956725-17956744 MS.gene028347:CDS 40.0%
! TATATAGAAGCGACTGGTTG+GGG - chr4.2:17957808-17957827 MS.gene028347:CDS 40.0%
!! CTATTTTGGGCTATAAGAGG+TGG - chr4.2:17957013-17957032 MS.gene028347:CDS 40.0%
!! CTGAATTCAGGTTGAGACAT+TGG + chr4.2:17956439-17956458 None:intergenic 40.0%
AAATTACGGCTGCTTGGACA+TGG + chr4.2:17956607-17956626 None:intergenic 45.0%
AACACTGCTTGGGTTCAATC+CGG - chr4.2:17956770-17956789 MS.gene028347:CDS 45.0%
AATTACGGCTGCTTGGACAT+GGG + chr4.2:17956606-17956625 None:intergenic 45.0%
ACTTCAGGTGGTGTAACACT+TGG + chr4.2:17957403-17957422 None:intergenic 45.0%
AGGGAACCAATACCAGAAAC+TGG - chr4.2:17957481-17957500 MS.gene028347:CDS 45.0%
CATTCTCGACAGAAAAGCCA+TGG - chr4.2:17956982-17957001 MS.gene028347:CDS 45.0%
CCAAAATAGATCCTCTCCCA+TGG + chr4.2:17957002-17957021 None:intergenic 45.0%
CTATGCACAAGCTTAGGTGT+CGG - chr4.2:17956860-17956879 MS.gene028347:CDS 45.0%
GCTATATAGAAGCGACTGGT+TGG - chr4.2:17957806-17957825 MS.gene028347:CDS 45.0%
GCTCGAATCGTTGATGCAAA+TGG - chr4.2:17956956-17956975 MS.gene028347:CDS 45.0%
GTATTGAAGGATTGTGGCAG+AGG - chr4.2:17957503-17957522 MS.gene028347:CDS 45.0%
TCCCTGAATTGGGTTTGACA+AGG - chr4.2:17957323-17957342 MS.gene028347:CDS 45.0%
TGCTTGGACATGGGAATCTT+GGG + chr4.2:17956597-17956616 None:intergenic 45.0%
TTGTTTCCAACACTAGCAGC+TGG + chr4.2:17957220-17957239 None:intergenic 45.0%
! CACATAACAGGAGGTGCATA+TGG - chr4.2:17956887-17956906 MS.gene028347:CDS 45.0%
! CCATGGGAGAGGATCTATTT+TGG - chr4.2:17956999-17957018 MS.gene028347:CDS 45.0%
! TAGATGGTGCCAAACACCTT+AGG + chr4.2:17956544-17956563 None:intergenic 45.0%
! TTTTGGGCTATAAGAGGTGG+TGG - chr4.2:17957016-17957035 MS.gene028347:CDS 45.0%
AAGCTTGTGCATAGACCTGC+AGG + chr4.2:17956854-17956873 None:intergenic 50.0%
AGCTTGTGCATAGACCTGCA+GGG + chr4.2:17956853-17956872 None:intergenic 50.0%
AGTGGTGGCCATGACTATGA+AGG - chr4.2:17956662-17956681 MS.gene028347:CDS 50.0%
ATCCAACCAGCTGCTAGTGT+TGG - chr4.2:17957211-17957230 MS.gene028347:CDS 50.0%
CTGCTTGGACATGGGAATCT+TGG + chr4.2:17956598-17956617 None:intergenic 50.0%
GCAGGTCTATGCACAAGCTT+AGG - chr4.2:17956854-17956873 MS.gene028347:CDS 50.0%
GGATTGAACCCAAGCAGTGT+TGG + chr4.2:17956771-17956790 None:intergenic 50.0%
TGGACATGGGAATCTTGGGA+GGG + chr4.2:17956593-17956612 None:intergenic 50.0%
TGGAGCAAATTACGGCTGCT+TGG + chr4.2:17956613-17956632 None:intergenic 50.0%
TTCCAACACTAGCAGCTGGT+TGG + chr4.2:17957216-17957235 None:intergenic 50.0%
TTGGACATGGGAATCTTGGG+AGG + chr4.2:17956594-17956613 None:intergenic 50.0%
! AAGCGACTGGTTGGGGTTAT+AGG - chr4.2:17957815-17957834 MS.gene028347:CDS 50.0%
! ACTGTCACTGTTGGTGGAAC+AGG + chr4.2:17957088-17957107 None:intergenic 50.0%
!! GTTCAATCCGGTGCCACTAT+CGG - chr4.2:17956782-17956801 MS.gene028347:CDS 50.0%
CGACAGAAAAGCCATGGGAG+AGG - chr4.2:17956988-17957007 MS.gene028347:CDS 55.0%
GTCGGTGGACACATAACAGG+AGG - chr4.2:17956878-17956897 MS.gene028347:CDS 55.0%
TCGGTTCTTGGTTACCCTGC+AGG - chr4.2:17956836-17956855 MS.gene028347:CDS 55.0%
! GACTTCACCGATAGTGGCAC+CGG + chr4.2:17956792-17956811 None:intergenic 55.0%
! TGGGCTATAAGAGGTGGTGG+AGG - chr4.2:17957019-17957038 MS.gene028347:CDS 55.0%
!! GCTATAAGAGGTGGTGGAGG+TGG - chr4.2:17957022-17957041 MS.gene028347:CDS 55.0%
!! GGTGTCGGTGGACACATAAC+AGG - chr4.2:17956875-17956894 MS.gene028347:CDS 55.0%
!! TGCACAAGCTTAGGTGTCGG+TGG - chr4.2:17956863-17956882 MS.gene028347:CDS 55.0%
!! GGTGGTGGAGGTGGAAGCTT+TGG - chr4.2:17957031-17957050 MS.gene028347:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17956341 17957939 17956341 ID=MS.gene028347
chr4.2 mRNA 17956341 17957939 17956341 ID=MS.gene028347.t1;Parent=MS.gene028347
chr4.2 exon 17956341 17957939 17956341 ID=MS.gene028347.t1.exon1;Parent=MS.gene028347.t1
chr4.2 CDS 17956341 17957939 17956341 ID=cds.MS.gene028347.t1;Parent=MS.gene028347.t1
Gene Sequence

>MS.gene028347

ATGGTGTCACCAATCTTACCCTTGCCAATTTTAGTCCTTCTATTATCATCACTTTCATTAAAAGTTTCCTCTTTATTACTTCAAGACAGTTTTGTCCAATGTCTCAACCTGAATTCAGACAAAACATATCCATTTCATACAACAATTTACACTCCAAATGAACCTTCATTCTCAACCATCCTTGATTCCTTAGCAATGAACCTAAGGTGTTTGGCACCATCTACACCGAAACCTGAATTCATATTCACACCCTCCCAAGATTCCCATGTCCAAGCAGCCGTAATTTGCTCCAAAAAACTTGGAATTCATCTAAGAGTAAGAAGTGGTGGCCATGACTATGAAGGACTTTCATATGTTTCCAAAATCGAAACACCTTTCATAGTTGTCGATTTAGTCAAACTTCGCGGCATAAGCGTCGACACTAAAACCAACACTGCTTGGGTTCAATCCGGTGCCACTATCGGTGAAGTCTATTACAGAATATACGAGAAAAGTTCGGTTCTTGGTTACCCTGCAGGTCTATGCACAAGCTTAGGTGTCGGTGGACACATAACAGGAGGTGCATATGGAACATTGATGAGAAAATATGGTCTTGGTGTTGATAATGTCTTAGATGCTCGAATCGTTGATGCAAATGGAAACATTCTCGACAGAAAAGCCATGGGAGAGGATCTATTTTGGGCTATAAGAGGTGGTGGAGGTGGAAGCTTTGGTATACTTCTTTGGTGGAAGATAAAGCTTGTTCCTGTTCCACCAACAGTGACAGTTTTTACTGTCACTAAAACTCTAGAACAAGGTGCTACAAAAATTCTTCATAGATGGCAAGAAGTAGCACCTTTTATTGATGAAAATCTCTTTATTAGAGTCATCATCCAACCAGCTGCTAGTGTTGGAAACAAAACTCAAAGAACTATTACAACTTCTTACAATGCTGTTTTTCTTGGTGAAGCAAGAACACTACTTCAAGTCATGAAAACAAGTTTCCCTGAATTGGGTTTGACAAGGAAAGATTGTCAAGAAACTAGTTGGATCAAATCTGTTCTCTATATTGCTGGATATCCAAGTGTTACACCACCTGAAGTTCTTCTTAAAGGAAAACCAACATTCAAGAATTCTTTCAAAGCAAAATCAGATTTTGTGAGGGAACCAATACCAGAAACTGGTATTGAAGGATTGTGGCAGAGGCTGTTGAGTGAAGATAGCCCTTTGATGATTTGGAATCCATATGGTGGAAAAATGAGTGAATTTTCAGAATCTGATAGCCCTTTTCCACATAGAAATGGAACATTGTATAAAATTCAGTACTTGTCTATATGGCAAGAGGGTGACAAGAATGTTGCAAAGCATGTAGACTGGATTAGGAAACTTTATAACTATATGACTCCTTATGTGTCAAAGTTTCCAAGGGAAGCTTATGTGAATTATAGGGATCTTGATTTGGGAATTAACTCTAAGAATAGTACAAGCTATATAGAAGCGACTGGTTGGGGTTATAGGTATTTTAAGGGTAATTTTAACAAGTTGGTTCAGATAAAGACTAGAGTGGATCCTGAAAATGTGTTTAGGCATGAACAAAGTATTCCACCACTTCCAATATAA
Protein sequence

>MS.gene028347.t1

MVSPILPLPILVLLLSSLSLKVSSLLLQDSFVQCLNLNSDKTYPFHTTIYTPNEPSFSTILDSLAMNLRCLAPSTPKPEFIFTPSQDSHVQAAVICSKKLGIHLRVRSGGHDYEGLSYVSKIETPFIVVDLVKLRGISVDTKTNTAWVQSGATIGEVYYRIYEKSSVLGYPAGLCTSLGVGGHITGGAYGTLMRKYGLGVDNVLDARIVDANGNILDRKAMGEDLFWAIRGGGGGSFGILLWWKIKLVPVPPTVTVFTVTKTLEQGATKILHRWQEVAPFIDENLFIRVIIQPAASVGNKTQRTITTSYNAVFLGEARTLLQVMKTSFPELGLTRKDCQETSWIKSVLYIAGYPSVTPPEVLLKGKPTFKNSFKAKSDFVREPIPETGIEGLWQRLLSEDSPLMIWNPYGGKMSEFSESDSPFPHRNGTLYKIQYLSIWQEGDKNVAKHVDWIRKLYNYMTPYVSKFPREAYVNYRDLDLGINSKNSTSYIEATGWGYRYFKGNFNKLVQIKTRVDPENVFRHEQSIPPLPI