AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene028346

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028346.t1 MTR_4g094520 98.305 531 9 0 1 531 6 536 0.0 1085
MS.gene028346.t1 MTR_2g031390 71.132 530 152 1 1 529 2 531 0.0 777
MS.gene028346.t1 MTR_4g094518 70.276 508 144 2 26 529 28 532 0.0 710
MS.gene028346.t1 MTR_3g009820 50.947 528 248 8 5 527 1 522 0.0 543
MS.gene028346.t1 MTR_3g009760 50.947 528 248 8 5 527 1 522 0.0 540
MS.gene028346.t1 MTR_4g094488 52.700 537 237 9 4 525 1 535 0.0 540
MS.gene028346.t1 MTR_2g031590 49.713 523 255 4 12 528 13 533 0.0 537
MS.gene028346.t1 MTR_4g094492 50.566 530 252 6 5 527 10 536 0.0 536
MS.gene028346.t1 MTR_4g094478 51.955 537 243 7 4 527 1 535 0.0 535
MS.gene028346.t1 MTR_4g094480 50.758 528 251 6 4 525 9 533 0.0 530
MS.gene028346.t1 MTR_2g031560 48.381 525 265 5 7 527 1 523 0.0 530
MS.gene028346.t1 MTR_4g094498 49.628 538 256 5 1 527 1 534 0.0 529
MS.gene028346.t1 MTR_3g009880 50.760 526 250 7 5 527 1 520 0.0 528
MS.gene028346.t1 MTR_2g031420 52.953 508 233 5 21 527 25 527 0.0 523
MS.gene028346.t1 MTR_4g094495 50.473 529 250 7 7 525 12 538 0.0 522
MS.gene028346.t1 MTR_2g031460 49.904 523 254 6 8 527 10 527 1.56e-178 513
MS.gene028346.t1 MTR_4g094515 50.189 530 254 4 7 528 624 1151 7.78e-174 521
MS.gene028346.t1 MTR_4g094515 48.282 524 257 6 13 528 24 541 2.29e-156 476
MS.gene028346.t1 MTR_4g091150 51.277 509 235 8 27 527 37 540 3.39e-173 500
MS.gene028346.t1 MTR_2g031510 49.412 510 250 5 24 528 35 541 8.52e-171 494
MS.gene028346.t1 MTR_4g094418 54.462 437 188 6 100 527 1 435 1.11e-170 496
MS.gene028346.t1 MTR_2g031490 48.381 525 258 6 14 528 18 539 1.38e-167 486
MS.gene028346.t1 MTR_4g091200 47.368 532 262 8 1 525 4 524 1.98e-167 485
MS.gene028346.t1 MTR_6g017205 48.000 525 257 8 7 525 10 524 3.94e-166 482
MS.gene028346.t1 MTR_4g091190 46.792 530 270 7 1 525 4 526 4.89e-165 479
MS.gene028346.t1 MTR_4g088355 48.611 504 247 7 29 525 33 531 6.81e-163 474
MS.gene028346.t1 MTR_4g057990 46.970 528 269 7 1 525 1 520 9.16e-163 473
MS.gene028346.t1 MTR_4g091170 47.368 456 213 7 75 525 107 540 6.69e-150 442
MS.gene028346.t1 MTR_2g023590 47.097 465 239 5 64 525 4 464 1.75e-147 432
MS.gene028346.t1 MTR_1g076830 39.238 525 303 9 15 530 21 538 1.50e-119 363
MS.gene028346.t1 MTR_2g031600 53.648 233 106 1 12 244 14 244 1.26e-79 255
MS.gene028346.t1 MTR_2g031600 47.531 162 84 1 368 528 240 401 3.01e-47 169
MS.gene028346.t1 MTR_3g009850 51.754 228 108 2 302 527 1 228 4.86e-79 248
MS.gene028346.t1 MTR_2g084370 41.441 111 43 2 126 236 1 89 4.82e-26 102
MS.gene028346.t1 MTR_8g062280 46.392 97 24 1 428 524 4 72 2.07e-22 91.7
MS.gene028346.t1 MTR_2g084405 50.633 79 39 0 126 204 1 79 2.64e-19 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene028346.t1 AT1G30760 64.752 505 171 3 24 525 48 548 0.0 665
MS.gene028346.t1 AT1G30760 64.752 505 171 3 24 525 30 530 0.0 664
MS.gene028346.t1 AT2G34790 62.141 523 190 4 6 525 11 528 0.0 661
MS.gene028346.t1 AT5G44400 60.829 531 198 7 4 527 9 536 0.0 652
MS.gene028346.t1 AT5G44390 56.262 535 223 7 3 529 11 542 0.0 604
MS.gene028346.t1 AT5G44380 57.558 516 212 6 18 528 67 580 0.0 579
MS.gene028346.t1 AT5G44380 57.558 516 212 6 18 528 27 540 0.0 577
MS.gene028346.t1 AT1G30700 50.095 525 256 4 7 528 6 527 5.53e-177 509
MS.gene028346.t1 AT5G44360 48.496 532 259 9 5 529 9 532 2.75e-169 489
MS.gene028346.t1 AT5G44360 48.496 532 259 9 5 529 25 548 4.34e-169 490
MS.gene028346.t1 AT1G26380 48.674 528 259 7 6 527 4 525 6.25e-168 486
MS.gene028346.t1 AT4G20860 48.833 514 249 8 22 527 22 529 1.68e-167 485
MS.gene028346.t1 AT4G20800 49.004 502 250 4 27 526 27 524 7.61e-166 481
MS.gene028346.t1 AT4G20820 47.547 530 261 8 3 527 9 526 2.72e-165 479
MS.gene028346.t1 AT1G26400 47.637 529 268 6 1 526 1 523 5.11e-164 476
MS.gene028346.t1 AT1G26420 47.810 525 261 6 14 529 7 527 8.71e-164 476
MS.gene028346.t1 AT1G11770 46.916 535 268 7 5 528 1 530 4.87e-163 474
MS.gene028346.t1 AT1G26390 48.810 504 252 4 29 529 29 529 7.27e-162 471
MS.gene028346.t1 AT1G26410 47.714 503 257 5 29 529 53 551 1.38e-160 468
MS.gene028346.t1 AT1G34575 45.180 529 264 10 7 525 10 522 6.53e-160 466
MS.gene028346.t1 AT1G30730 46.110 527 275 6 4 525 1 523 4.52e-159 463
MS.gene028346.t1 AT4G20840 48.323 507 253 6 25 527 34 535 4.68e-159 464
MS.gene028346.t1 AT1G30710 45.247 526 268 9 12 527 13 528 2.52e-158 462
MS.gene028346.t1 AT5G44440 45.301 532 277 7 1 525 3 527 2.36e-156 457
MS.gene028346.t1 AT1G30720 46.784 513 266 5 16 525 16 524 4.67e-156 456
MS.gene028346.t1 AT5G44440 45.301 532 277 7 1 525 73 597 3.10e-155 456
MS.gene028346.t1 AT5G44410 47.243 544 251 12 1 525 1 527 1.09e-151 445
MS.gene028346.t1 AT1G30740 47.419 523 258 8 15 528 18 532 8.53e-151 442
MS.gene028346.t1 AT1G30740 47.419 523 258 8 15 528 15 529 1.05e-150 442
MS.gene028346.t1 AT4G20830 47.451 510 259 6 22 527 32 536 4.78e-150 441
MS.gene028346.t1 AT4G20830 47.451 510 259 6 22 527 32 536 1.91e-149 440
MS.gene028346.t1 AT1G01980 46.899 516 260 8 22 528 25 535 1.14e-148 437
MS.gene028346.t1 AT2G34810 44.788 518 268 9 22 527 28 539 8.81e-143 422

Find 119 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGGGAGAAGACCTATTT+TGG 0.145172 4.2:-17953002 MS.gene028346:CDS
TTGCTAACTCTTGGGGTTAT+AGG 0.226534 4.2:-17952189 MS.gene028346:CDS
TACAAAATTCAGTACTTATC+TGG 0.232561 4.2:-17952373 MS.gene028346:CDS
AGGGAATTGAGGGAAGTTTC+TGG 0.233163 4.2:+17952511 None:intergenic
GATTGAAGTGTAAGACTTAT+TGG 0.240801 4.2:+17953503 None:intergenic
GCAGGCCTTTGTACATCTTT+AGG 0.242864 4.2:-17953147 MS.gene028346:CDS
TATAGATTGAAGAGGGAAAT+TGG 0.267535 4.2:+17953532 None:intergenic
TTTCTTGTTAAACCCAATTC+AGG 0.270282 4.2:+17952679 None:intergenic
TTTCCCTGGAGCAGAACTTC+TGG 0.282559 4.2:+17952589 None:intergenic
TTGATCCATCTAATTTCTTT+AGG 0.288821 4.2:-17952120 MS.gene028346:CDS
GTCTTTCTCTTGCTAACTCT+TGG 0.294277 4.2:-17952198 MS.gene028346:CDS
TCTTACCAAGCTCTATTTCT+TGG 0.303983 4.2:-17952742 MS.gene028346:CDS
TAGGGATTCCATATCATTAA+AGG 0.306572 4.2:+17952460 None:intergenic
ATGATGGTCCTTTAATGATA+TGG 0.309466 4.2:-17952468 MS.gene028346:CDS
GCTAAAATCGTTAATGCTAA+TGG 0.309493 4.2:-17953045 MS.gene028346:CDS
CCAGGCAATGGTAGTGTTCC+TGG 0.320965 4.2:-17952784 MS.gene028346:CDS
TCTTTCTCTTGCTAACTCTT+GGG 0.323097 4.2:-17952197 MS.gene028346:CDS
TTTAACAAGAAAGGATTGTT+TGG 0.345762 4.2:-17952668 MS.gene028346:CDS
TCCGCGAAAAGTCCAGTTCA+TGG 0.354395 4.2:-17953177 MS.gene028346:CDS
ACTTCTGGAGGTGTTGTATT+GGG 0.356109 4.2:+17952604 None:intergenic
AATTATAGAGACTTGGATAT+AGG 0.357847 4.2:-17952244 MS.gene028346:CDS
GATTGATGGAGAAAAGAATA+TGG 0.359004 4.2:-17952347 MS.gene028346:CDS
GGAGGCGGAGGAGGCAGCTT+CGG 0.369626 4.2:-17952970 MS.gene028346:CDS
ATTGAAGAGGGAAATTGGTT+TGG 0.371420 4.2:+17953537 None:intergenic
GTGAATATGAAATCTGGTTT+TGG 0.372628 4.2:+17953435 None:intergenic
GAAACAAGCTTCTTTATAGA+TGG 0.373043 4.2:-17952861 MS.gene028346:CDS
TCTCTGGTGGAAGATCAAAT+TGG 0.387633 4.2:-17952941 MS.gene028346:CDS
TGAATTGGGTTTAACAAGAA+AGG 0.388314 4.2:-17952677 MS.gene028346:CDS
GAATTTGAGCATGAAGTGAT+AGG 0.400466 4.2:+17953559 None:intergenic
ACACTTTCTTACTTACCAAT+TGG 0.404020 4.2:+17952073 None:intergenic
ATTCAAGTGGGAGCAACTAT+TGG 0.406609 4.2:-17953219 MS.gene028346:CDS
CTCATTACCGGAGGTGCTTA+CGG 0.407070 4.2:-17953114 MS.gene028346:CDS
AGGATTGTTTGGAAACTAGT+TGG 0.409589 4.2:-17952657 MS.gene028346:CDS
ATTATAGAGACTTGGATATA+GGG 0.410356 4.2:-17952243 MS.gene028346:CDS
TACTTATCTGGATGGATTGA+TGG 0.410447 4.2:-17952361 MS.gene028346:CDS
GAAAACTCTGCTTCATAACT+TGG 0.419933 4.2:+17952701 None:intergenic
AACTTCTGGAGGTGTTGTAT+TGG 0.421717 4.2:+17952603 None:intergenic
CCAGTTCATGGCTTCCCTGC+AGG 0.433260 4.2:-17953165 MS.gene028346:CDS
GGTTCTATGATGAGAAAGTA+TGG 0.433505 4.2:-17953093 MS.gene028346:CDS
GGTATCGGTGGCCTCATTAC+CGG 0.435936 4.2:-17953126 MS.gene028346:CDS
CCTGCAGGGAAGCCATGAAC+TGG 0.437854 4.2:+17953165 None:intergenic
TACCAAGCTCTATTTCTTGG+TGG 0.445176 4.2:-17952739 MS.gene028346:CDS
TGATGCATAAGGAGTCATAT+AGG 0.456214 4.2:+17952291 None:intergenic
CCTACTCATCATTCCTCCAT+AGG 0.458631 4.2:+17952441 None:intergenic
CTTTCTCTTGCTAACTCTTG+GGG 0.458680 4.2:-17952196 MS.gene028346:CDS
CTCCTCTTATTGCCCAAAAT+AGG 0.459368 4.2:+17952989 None:intergenic
GCAGCTTCGGAGTTATTCTC+TGG 0.461190 4.2:-17952957 MS.gene028346:CDS
GCAATAAGAGGAGGCGGAGG+AGG 0.463947 4.2:-17952979 MS.gene028346:CDS
GTTCCTTCTCTGTGAGGAAA+AGG 0.464878 4.2:+17952400 None:intergenic
GGAAACTAGTTGGATCAAAT+CGG 0.466810 4.2:-17952647 MS.gene028346:CDS
AGGTGTTGTATTGGGATAAC+CGG 0.468626 4.2:+17952612 None:intergenic
CTTTGTACATCTTTAGGTAT+CGG 0.472738 4.2:-17953141 MS.gene028346:CDS
TCATGCCTAAAGAAATTAGA+TGG 0.473346 4.2:+17952115 None:intergenic
ACCTCCAGAAGTTCTGCTCC+AGG 0.474916 4.2:-17952593 MS.gene028346:CDS
ATATTCCACATAACACGGCT+TGG 0.479287 4.2:-17953242 MS.gene028346:CDS
AAAGATGTACAAAGGCCTGC+AGG 0.483734 4.2:+17953150 None:intergenic
TTGAAGTAAGCCTTGGATGT+AGG 0.484349 4.2:+17952565 None:intergenic
TTCTTCAATTGAAACTGAAG+TGG 0.484924 4.2:+17953596 None:intergenic
CTGAAGGCAATAGATACCTT+AGG 0.489308 4.2:+17953460 None:intergenic
CCCTGGAGCAGAACTTCTGG+AGG 0.490999 4.2:+17952592 None:intergenic
TACTTACCAATTGGAAGTGG+AGG 0.497327 4.2:+17952082 None:intergenic
CATAGAACCGTAAGCACCTC+CGG 0.501126 4.2:+17953107 None:intergenic
AAACTTCCCTCAATTCCCTA+TGG 0.503759 4.2:-17952507 MS.gene028346:CDS
AATGGTGTGAATATGAAATC+TGG 0.504260 4.2:+17953429 None:intergenic
CTATGTGAATTATAGAGACT+TGG 0.504659 4.2:-17952251 MS.gene028346:CDS
TGAATCCAAGCCGTGTTATG+TGG 0.508263 4.2:+17953237 None:intergenic
GAACTACGAAAGTTGCTAAG+AGG 0.515266 4.2:+17953637 None:intergenic
CCAGGAACACTACCATTGCC+TGG 0.516099 4.2:+17952784 None:intergenic
AGTATCCCTCCACTTCCAAT+TGG 0.521965 4.2:-17952088 MS.gene028346:CDS
CGTAAGCACCTCCGGTAATG+AGG 0.522399 4.2:+17953115 None:intergenic
CACGTAGTTTAGCCAGGTCT+AGG 0.524645 4.2:+17953277 None:intergenic
CTCCACCAAGAAATAGAGCT+TGG 0.526513 4.2:+17952737 None:intergenic
TCTTACTTACCAATTGGAAG+TGG 0.527064 4.2:+17952079 None:intergenic
TTAATGATATGGAATCCCTA+TGG 0.528439 4.2:-17952457 MS.gene028346:CDS
ATTCTTGACAGAAAAGCAAT+GGG 0.529934 4.2:-17953018 MS.gene028346:CDS
ATATGGCAAAACATATGAGT+TGG 0.530345 4.2:-17952330 MS.gene028346:CDS
ATTGAAACTGAAGTGGAAAG+TGG 0.535886 4.2:+17953603 None:intergenic
AAATCGGTTCTTTATATCGC+CGG 0.536045 4.2:-17952631 MS.gene028346:CDS
GCTTCGGAGTTATTCTCTGG+TGG 0.536428 4.2:-17952954 MS.gene028346:CDS
TTACTGCACGTAGTTTAGCC+AGG 0.536709 4.2:+17953271 None:intergenic
CTACTCATCATTCCTCCATA+GGG 0.537440 4.2:+17952442 None:intergenic
AAATTCAGTACTTATCTGGA+TGG 0.539864 4.2:-17952369 MS.gene028346:CDS
CGTTGATATTCCACATAACA+CGG 0.539970 4.2:-17953247 MS.gene028346:CDS
TAACTTCAATAGGTTAGTAA+AGG 0.543846 4.2:-17952155 MS.gene028346:CDS
AATTCTTGACAGAAAAGCAA+TGG 0.545747 4.2:-17953019 MS.gene028346:CDS
TTGCACATATCACAGCTGCT+TGG 0.546395 4.2:+17953391 None:intergenic
ATGATGAGAAAGTATGGCCT+CGG 0.549358 4.2:-17953087 MS.gene028346:CDS
CTGCTTGGACTTGTGAGTCA+TGG 0.555171 4.2:+17953406 None:intergenic
AGTGTAAGACTTATTGGTTG+AGG 0.555975 4.2:+17953509 None:intergenic
AGCGGAGGACACGACTATGA+AGG 0.557599 4.2:-17953339 MS.gene028346:CDS
GTTGAGGTATAGATTGAAGA+GGG 0.560414 4.2:+17953525 None:intergenic
AATCCACTGCTCAAAACCTA+AGG 0.560820 4.2:-17953476 MS.gene028346:CDS
ACCGTTCATGATCCTAGACC+TGG 0.569999 4.2:-17953289 MS.gene028346:CDS
TGGGCAATAAGAGGAGGCGG+AGG 0.574351 4.2:-17952982 MS.gene028346:CDS
AACTACGAAAGTTGCTAAGA+GGG 0.580812 4.2:+17953638 None:intergenic
TGTACATCTTTAGGTATCGG+TGG 0.582234 4.2:-17953138 MS.gene028346:CDS
CCTATGGAGGAATGATGAGT+AGG 0.584439 4.2:-17952441 MS.gene028346:CDS
CAAAACATATGAGTTGGATG+AGG 0.585356 4.2:-17952324 MS.gene028346:CDS
GTCTTGATTCAACCAGGCAA+TGG 0.587697 4.2:-17952796 MS.gene028346:CDS
GCCAGGTCTAGGATCATGAA+CGG 0.594071 4.2:+17953288 None:intergenic
GTAGTTACAGTTCTTTGACC+AGG 0.595410 4.2:+17952766 None:intergenic
ATCAGAGTCTTGATTCAACC+AGG 0.596708 4.2:-17952802 MS.gene028346:CDS
ACTTACCAATTGGAAGTGGA+GGG 0.597383 4.2:+17952083 None:intergenic
TGGACTTGTGAGTCATGGAA+TGG 0.604528 4.2:+17953411 None:intergenic
AAGATGTACAAAGGCCTGCA+GGG 0.604713 4.2:+17953151 None:intergenic
CCAGGGAAAACCTACATCCA+AGG 0.606686 4.2:-17952575 MS.gene028346:CDS
CCTCCAGAAGTTCTGCTCCA+GGG 0.608247 4.2:-17952592 MS.gene028346:CDS
GGTTGAGGTATAGATTGAAG+AGG 0.611987 4.2:+17953524 None:intergenic
TAAAGAGTTCCTTCTCTGTG+AGG 0.618545 4.2:+17952394 None:intergenic
ATCGGTGGCCTCATTACCGG+AGG 0.636534 4.2:-17953123 MS.gene028346:CDS
ATGATATGGAATCCCTATGG+AGG 0.647237 4.2:-17952454 MS.gene028346:CDS
AACACGGCTTGGATTCAAGT+GGG 0.650619 4.2:-17953231 MS.gene028346:CDS
TAACACGGCTTGGATTCAAG+TGG 0.656178 4.2:-17953232 MS.gene028346:CDS
CATATGAGAATACGAAGCGG+AGG 0.688013 4.2:-17953354 MS.gene028346:CDS
CGATACCTAAAGATGTACAA+AGG 0.714905 4.2:+17953142 None:intergenic
ACAAAGAGTCTAGAACAAGG+TGG 0.730420 4.2:-17952883 MS.gene028346:CDS
ATTCATATGAGAATACGAAG+CGG 0.748676 4.2:-17953357 MS.gene028346:CDS
GTAACAAAGAGTCTAGAACA+AGG 0.765309 4.2:-17952886 MS.gene028346:CDS
CAACAACGTTGTCAACACCG+AGG 0.799916 4.2:+17953070 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTTCTTCAAGATGA+TGG - chr4.2:17953251-17953270 MS.gene028346:CDS 20.0%
!!! AAGAGATTTTCATCAATATT+TGG + chr4.2:17952912-17952931 None:intergenic 20.0%
!!! TTGAAGAAAAATTTTCCATA+GGG + chr4.2:17953246-17953265 None:intergenic 20.0%
! AATTATAGAGACTTGGATAT+AGG - chr4.2:17953491-17953510 MS.gene028346:CDS 25.0%
! ATTATAGAGACTTGGATATA+GGG - chr4.2:17953492-17953511 MS.gene028346:CDS 25.0%
! TAACTTCAATAGGTTAGTAA+AGG - chr4.2:17953580-17953599 MS.gene028346:CDS 25.0%
! TACAAAATTCAGTACTTATC+TGG - chr4.2:17953362-17953381 MS.gene028346:CDS 25.0%
! TTATGCATCAAAAAATCCAA+GGG - chr4.2:17953457-17953476 MS.gene028346:CDS 25.0%
! TTGATCCATCTAATTTCTTT+AGG - chr4.2:17953615-17953634 MS.gene028346:CDS 25.0%
! TTTAACAAGAAAGGATTGTT+TGG - chr4.2:17953067-17953086 MS.gene028346:CDS 25.0%
!! CTTGAAGAAAAATTTTCCAT+AGG + chr4.2:17953247-17953266 None:intergenic 25.0%
!!! ATTTTAAGGGTAACTTCAAT+AGG - chr4.2:17953570-17953589 MS.gene028346:CDS 25.0%
AAATTCAGTACTTATCTGGA+TGG - chr4.2:17953366-17953385 MS.gene028346:CDS 30.0%
AATGGTGTGAATATGAAATC+TGG + chr4.2:17952309-17952328 None:intergenic 30.0%
AATTCTTGACAGAAAAGCAA+TGG - chr4.2:17952716-17952735 MS.gene028346:CDS 30.0%
ATATGGCAAAACATATGAGT+TGG - chr4.2:17953405-17953424 MS.gene028346:CDS 30.0%
ATGATGGTCCTTTAATGATA+TGG - chr4.2:17953267-17953286 MS.gene028346:CDS 30.0%
ATTCATATGAGAATACGAAG+CGG - chr4.2:17952378-17952397 MS.gene028346:CDS 30.0%
ATTCTTGACAGAAAAGCAAT+GGG - chr4.2:17952717-17952736 MS.gene028346:CDS 30.0%
CTATGTGAATTATAGAGACT+TGG - chr4.2:17953484-17953503 MS.gene028346:CDS 30.0%
CTTATGCATCAAAAAATCCA+AGG - chr4.2:17953456-17953475 MS.gene028346:CDS 30.0%
CTTTGTACATCTTTAGGTAT+CGG - chr4.2:17952594-17952613 MS.gene028346:CDS 30.0%
GAAACAAGCTTCTTTATAGA+TGG - chr4.2:17952874-17952893 MS.gene028346:CDS 30.0%
GATTGAAGTGTAAGACTTAT+TGG + chr4.2:17952235-17952254 None:intergenic 30.0%
GATTGATGGAGAAAAGAATA+TGG - chr4.2:17953388-17953407 MS.gene028346:CDS 30.0%
GCTAAAATCGTTAATGCTAA+TGG - chr4.2:17952690-17952709 MS.gene028346:CDS 30.0%
GTGATATGTGCAAAAAAACA+TGG - chr4.2:17952354-17952373 MS.gene028346:CDS 30.0%
TAGGGATTCCATATCATTAA+AGG + chr4.2:17953278-17953297 None:intergenic 30.0%
TATAGATTGAAGAGGGAAAT+TGG + chr4.2:17952206-17952225 None:intergenic 30.0%
TCATGCCTAAAGAAATTAGA+TGG + chr4.2:17953623-17953642 None:intergenic 30.0%
TGAATTGGGTTTAACAAGAA+AGG - chr4.2:17953058-17953077 MS.gene028346:CDS 30.0%
TTAATGATATGGAATCCCTA+TGG - chr4.2:17953278-17953297 MS.gene028346:CDS 30.0%
TTCTTCAATTGAAACTGAAG+TGG + chr4.2:17952142-17952161 None:intergenic 30.0%
TTTCTTGTTAAACCCAATTC+AGG + chr4.2:17953059-17953078 None:intergenic 30.0%
! AAAAACTGTGACAGTTTTCA+CGG - chr4.2:17952827-17952846 MS.gene028346:CDS 30.0%
! AAATTTTCCATAGGGAATTG+AGG + chr4.2:17953238-17953257 None:intergenic 30.0%
! AATTTTCCATAGGGAATTGA+GGG + chr4.2:17953237-17953256 None:intergenic 30.0%
! TAATTCACATAGTTTTCCCT+TGG + chr4.2:17953476-17953495 None:intergenic 30.0%
! TTGGGGTTATAGGTATTTTA+AGG - chr4.2:17953556-17953575 MS.gene028346:CDS 30.0%
!! GTGAATATGAAATCTGGTTT+TGG + chr4.2:17952303-17952322 None:intergenic 30.0%
!! TGGGGTTATAGGTATTTTAA+GGG - chr4.2:17953557-17953576 MS.gene028346:CDS 30.0%
!!! CTTGGATTTTTTGATGCATA+AGG + chr4.2:17953458-17953477 None:intergenic 30.0%
AACTACGAAAGTTGCTAAGA+GGG + chr4.2:17952100-17952119 None:intergenic 35.0%
AGGATTGTTTGGAAACTAGT+TGG - chr4.2:17953078-17953097 MS.gene028346:CDS 35.0%
ATTGAAACTGAAGTGGAAAG+TGG + chr4.2:17952135-17952154 None:intergenic 35.0%
ATTGAAGAGGGAAATTGGTT+TGG + chr4.2:17952201-17952220 None:intergenic 35.0%
CGATACCTAAAGATGTACAA+AGG + chr4.2:17952596-17952615 None:intergenic 35.0%
CGTTGATATTCCACATAACA+CGG - chr4.2:17952488-17952507 MS.gene028346:CDS 35.0%
GAAAACTCTGCTTCATAACT+TGG + chr4.2:17953037-17953056 None:intergenic 35.0%
GAATTTGAGCATGAAGTGAT+AGG + chr4.2:17952179-17952198 None:intergenic 35.0%
GGAAACTAGTTGGATCAAAT+CGG - chr4.2:17953088-17953107 MS.gene028346:CDS 35.0%
GGTTCTATGATGAGAAAGTA+TGG - chr4.2:17952642-17952661 MS.gene028346:CDS 35.0%
GTTGAGGTATAGATTGAAGA+GGG + chr4.2:17952213-17952232 None:intergenic 35.0%
TCTTTCTCTTGCTAACTCTT+GGG - chr4.2:17953538-17953557 MS.gene028346:CDS 35.0%
TGATGCATAAGGAGTCATAT+AGG + chr4.2:17953447-17953466 None:intergenic 35.0%
! AAATCGGTTCTTTATATCGC+CGG - chr4.2:17953104-17953123 MS.gene028346:CDS 35.0%
! AAGCAGAGTTTTCCTGAATT+GGG - chr4.2:17953044-17953063 MS.gene028346:CDS 35.0%
! AATGGGAGAAGACCTATTTT+GGG - chr4.2:17952734-17952753 MS.gene028346:CDS 35.0%
! AGTGTAAGACTTATTGGTTG+AGG + chr4.2:17952229-17952248 None:intergenic 35.0%
! CAAAACATATGAGTTGGATG+AGG - chr4.2:17953411-17953430 MS.gene028346:CDS 35.0%
! GTAACAAAGAGTCTAGAACA+AGG - chr4.2:17952849-17952868 MS.gene028346:CDS 35.0%
! GTGAAAACTGTCACAGTTTT+TGG + chr4.2:17952828-17952847 None:intergenic 35.0%
! TCTTACCAAGCTCTATTTCT+TGG - chr4.2:17952993-17953012 MS.gene028346:CDS 35.0%
!! TACTTATCTGGATGGATTGA+TGG - chr4.2:17953374-17953393 MS.gene028346:CDS 35.0%
!!! TTTTGCTTTGAAGTAAGCCT+TGG + chr4.2:17953180-17953199 None:intergenic 35.0%
AAACTTCCCTCAATTCCCTA+TGG - chr4.2:17953228-17953247 MS.gene028346:CDS 40.0%
AATCCACTGCTCAAAACCTA+AGG - chr4.2:17952259-17952278 MS.gene028346:CDS 40.0%
ACTTACCAATTGGAAGTGGA+GGG + chr4.2:17953655-17953674 None:intergenic 40.0%
ATATTCCACATAACACGGCT+TGG - chr4.2:17952493-17952512 MS.gene028346:CDS 40.0%
ATCAGAGTCTTGATTCAACC+AGG - chr4.2:17952933-17952952 MS.gene028346:CDS 40.0%
ATGATATGGAATCCCTATGG+AGG - chr4.2:17953281-17953300 MS.gene028346:CDS 40.0%
ATTCAAGTGGGAGCAACTAT+TGG - chr4.2:17952516-17952535 MS.gene028346:CDS 40.0%
CTACTCATCATTCCTCCATA+GGG + chr4.2:17953296-17953315 None:intergenic 40.0%
CTCCTCTTATTGCCCAAAAT+AGG + chr4.2:17952749-17952768 None:intergenic 40.0%
CTGAAGGCAATAGATACCTT+AGG + chr4.2:17952278-17952297 None:intergenic 40.0%
CTTTCTCTTGCTAACTCTTG+GGG - chr4.2:17953539-17953558 MS.gene028346:CDS 40.0%
GAACTACGAAAGTTGCTAAG+AGG + chr4.2:17952101-17952120 None:intergenic 40.0%
GGTTGAGGTATAGATTGAAG+AGG + chr4.2:17952214-17952233 None:intergenic 40.0%
GTAGTTACAGTTCTTTGACC+AGG + chr4.2:17952972-17952991 None:intergenic 40.0%
GTCTTTCTCTTGCTAACTCT+TGG - chr4.2:17953537-17953556 MS.gene028346:CDS 40.0%
TAAAGAGTTCCTTCTCTGTG+AGG + chr4.2:17953344-17953363 None:intergenic 40.0%
TACTTACCAATTGGAAGTGG+AGG + chr4.2:17953656-17953675 None:intergenic 40.0%
TCTCTGGTGGAAGATCAAAT+TGG - chr4.2:17952794-17952813 MS.gene028346:CDS 40.0%
TTGAAGTAAGCCTTGGATGT+AGG + chr4.2:17953173-17953192 None:intergenic 40.0%
TTGCTAACTCTTGGGGTTAT+AGG - chr4.2:17953546-17953565 MS.gene028346:CDS 40.0%
! ACAAAGAGTCTAGAACAAGG+TGG - chr4.2:17952852-17952871 MS.gene028346:CDS 40.0%
! AGGTGTTGTATTGGGATAAC+CGG + chr4.2:17953126-17953145 None:intergenic 40.0%
! ATGATGAGAAAGTATGGCCT+CGG - chr4.2:17952648-17952667 MS.gene028346:CDS 40.0%
! CAATGGGAGAAGACCTATTT+TGG - chr4.2:17952733-17952752 MS.gene028346:CDS 40.0%
! GAAGCAGAGTTTTCCTGAAT+TGG - chr4.2:17953043-17953062 MS.gene028346:CDS 40.0%
! TACCAAGCTCTATTTCTTGG+TGG - chr4.2:17952996-17953015 MS.gene028346:CDS 40.0%
!! AACTTCTGGAGGTGTTGTAT+TGG + chr4.2:17953135-17953154 None:intergenic 40.0%
!! ACTTCTGGAGGTGTTGTATT+GGG + chr4.2:17953134-17953153 None:intergenic 40.0%
!! CTATTTTGGGCAATAAGAGG+AGG - chr4.2:17952747-17952766 MS.gene028346:CDS 40.0%
!! GACCTATTTTGGGCAATAAG+AGG - chr4.2:17952744-17952763 MS.gene028346:CDS 40.0%
!! TGTACATCTTTAGGTATCGG+TGG - chr4.2:17952597-17952616 MS.gene028346:CDS 40.0%
!!! AAATCTGGTTTTGGCACTGA+AGG + chr4.2:17952294-17952313 None:intergenic 40.0%
!!! ATACCTTAGGTTTTGAGCAG+TGG + chr4.2:17952265-17952284 None:intergenic 40.0%
AAAGATGTACAAAGGCCTGC+AGG + chr4.2:17952588-17952607 None:intergenic 45.0%
AAGATGTACAAAGGCCTGCA+GGG + chr4.2:17952587-17952606 None:intergenic 45.0%
AGGGAATTGAGGGAAGTTTC+TGG + chr4.2:17953227-17953246 None:intergenic 45.0%
AGTATCCCTCCACTTCCAAT+TGG - chr4.2:17953647-17953666 MS.gene028346:CDS 45.0%
CATATGAGAATACGAAGCGG+AGG - chr4.2:17952381-17952400 MS.gene028346:CDS 45.0%
CCTACTCATCATTCCTCCAT+AGG + chr4.2:17953297-17953316 None:intergenic 45.0%
CCTATGGAGGAATGATGAGT+AGG - chr4.2:17953294-17953313 MS.gene028346:CDS 45.0%
CTCCACCAAGAAATAGAGCT+TGG + chr4.2:17953001-17953020 None:intergenic 45.0%
GCAGGCCTTTGTACATCTTT+AGG - chr4.2:17952588-17952607 MS.gene028346:CDS 45.0%
GTCTTGATTCAACCAGGCAA+TGG - chr4.2:17952939-17952958 MS.gene028346:CDS 45.0%
GTTCCTTCTCTGTGAGGAAA+AGG + chr4.2:17953338-17953357 None:intergenic 45.0%
TGAATCCAAGCCGTGTTATG+TGG + chr4.2:17952501-17952520 None:intergenic 45.0%
TGGACTTGTGAGTCATGGAA+TGG + chr4.2:17952327-17952346 None:intergenic 45.0%
TTACTGCACGTAGTTTAGCC+AGG + chr4.2:17952467-17952486 None:intergenic 45.0%
TTGCACATATCACAGCTGCT+TGG + chr4.2:17952347-17952366 None:intergenic 45.0%
! AACACGGCTTGGATTCAAGT+GGG - chr4.2:17952504-17952523 MS.gene028346:CDS 45.0%
! ACACCTTTTCCTCACAGAGA+AGG - chr4.2:17953332-17953351 MS.gene028346:CDS 45.0%
! TAACACGGCTTGGATTCAAG+TGG - chr4.2:17952503-17952522 MS.gene028346:CDS 45.0%
! TTTTGGGCAATAAGAGGAGG+CGG - chr4.2:17952750-17952769 MS.gene028346:CDS 45.0%
!!! ACTGTCACAGTTTTTGGCAC+AGG + chr4.2:17952822-17952841 None:intergenic 45.0%
!!! GGTTTTGAGCAGTGGATTCA+AGG + chr4.2:17952257-17952276 None:intergenic 45.0%
ACCGTTCATGATCCTAGACC+TGG - chr4.2:17952446-17952465 MS.gene028346:CDS 50.0%
CAACAACGTTGTCAACACCG+AGG + chr4.2:17952668-17952687 None:intergenic 50.0%
CACGTAGTTTAGCCAGGTCT+AGG + chr4.2:17952461-17952480 None:intergenic 50.0%
CATAGAACCGTAAGCACCTC+CGG + chr4.2:17952631-17952650 None:intergenic 50.0%
CCAGGGAAAACCTACATCCA+AGG - chr4.2:17953160-17953179 MS.gene028346:CDS 50.0%
CTGCTTGGACTTGTGAGTCA+TGG + chr4.2:17952332-17952351 None:intergenic 50.0%
GCAGCTTCGGAGTTATTCTC+TGG - chr4.2:17952778-17952797 MS.gene028346:CDS 50.0%
GCTTCGGAGTTATTCTCTGG+TGG - chr4.2:17952781-17952800 MS.gene028346:CDS 50.0%
TCCGCGAAAAGTCCAGTTCA+TGG - chr4.2:17952558-17952577 MS.gene028346:CDS 50.0%
TTTCCCTGGAGCAGAACTTC+TGG + chr4.2:17953149-17953168 None:intergenic 50.0%
! CCTTGGATGTAGGTTTTCCC+TGG + chr4.2:17953163-17953182 None:intergenic 50.0%
! GCCAGGTCTAGGATCATGAA+CGG + chr4.2:17952450-17952469 None:intergenic 50.0%
! GCCATGAACTGGACTTTTCG+CGG + chr4.2:17952562-17952581 None:intergenic 50.0%
!! CTCATTACCGGAGGTGCTTA+CGG - chr4.2:17952621-17952640 MS.gene028346:CDS 50.0%
ACCTCCAGAAGTTCTGCTCC+AGG - chr4.2:17953142-17953161 MS.gene028346:CDS 55.0%
AGCGGAGGACACGACTATGA+AGG - chr4.2:17952396-17952415 MS.gene028346:CDS 55.0%
CCAGGAACACTACCATTGCC+TGG + chr4.2:17952954-17952973 None:intergenic 55.0%
CCTCCAGAAGTTCTGCTCCA+GGG - chr4.2:17953143-17953162 MS.gene028346:CDS 55.0%
CGTAAGCACCTCCGGTAATG+AGG + chr4.2:17952623-17952642 None:intergenic 55.0%
! CCAGGCAATGGTAGTGTTCC+TGG - chr4.2:17952951-17952970 MS.gene028346:CDS 55.0%
!! GGTATCGGTGGCCTCATTAC+CGG - chr4.2:17952609-17952628 MS.gene028346:CDS 55.0%
ATCGGTGGCCTCATTACCGG+AGG - chr4.2:17952612-17952631 MS.gene028346:CDS 60.0%
CCAGTTCATGGCTTCCCTGC+AGG - chr4.2:17952570-17952589 MS.gene028346:CDS 60.0%
CCCTGGAGCAGAACTTCTGG+AGG + chr4.2:17953146-17953165 None:intergenic 60.0%
CCTGCAGGGAAGCCATGAAC+TGG + chr4.2:17952573-17952592 None:intergenic 60.0%
GCAATAAGAGGAGGCGGAGG+AGG - chr4.2:17952756-17952775 MS.gene028346:CDS 60.0%
TGGGCAATAAGAGGAGGCGG+AGG - chr4.2:17952753-17952772 MS.gene028346:CDS 60.0%
GGAGGCGGAGGAGGCAGCTT+CGG - chr4.2:17952765-17952784 MS.gene028346:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 17952081 17953676 17952081 ID=MS.gene028346
chr4.2 mRNA 17952081 17953676 17952081 ID=MS.gene028346.t1;Parent=MS.gene028346
chr4.2 exon 17952081 17953676 17952081 ID=MS.gene028346.t1.exon1;Parent=MS.gene028346.t1
chr4.2 CDS 17952081 17953676 17952081 ID=cds.MS.gene028346.t1;Parent=MS.gene028346.t1
Gene Sequence

>MS.gene028346

ATGCAGTCTATGATGTCCCTCTTAGCAACTTTCGTAGTTCTGCTATCTGTTCCACTTTCCACTTCAGTTTCAATTGAAGAAATTTTCAATAATTGCCTATCACTTCATGCTCAAATTCCAAACCAATTTCCCTCTTCAATCTATACCTCAACCAATAAGTCTTACACTTCAATCCTTGAATCCACTGCTCAAAACCTAAGGTATCTATTGCCTTCAGTGCCAAAACCAGATTTCATATTCACACCATTCCATGACTCACAAGTCCAAGCAGCTGTGATATGTGCAAAAAAACATGGTATTCATATGAGAATACGAAGCGGAGGACACGACTATGAAGGTCTTTCTTATGTTTCTCTTATCGAGAAACCGTTCATGATCCTAGACCTGGCTAAACTACGTGCAGTAAACGTTGATATTCCACATAACACGGCTTGGATTCAAGTGGGAGCAACTATTGGTGAAGTCTATTACAAAATTTCCGCGAAAAGTCCAGTTCATGGCTTCCCTGCAGGCCTTTGTACATCTTTAGGTATCGGTGGCCTCATTACCGGAGGTGCTTACGGTTCTATGATGAGAAAGTATGGCCTCGGTGTTGACAACGTTGTTGACGCTAAAATCGTTAATGCTAATGGAAAAATTCTTGACAGAAAAGCAATGGGAGAAGACCTATTTTGGGCAATAAGAGGAGGCGGAGGAGGCAGCTTCGGAGTTATTCTCTGGTGGAAGATCAAATTGGTTCCTGTGCCAAAAACTGTGACAGTTTTCACGGTAACAAAGAGTCTAGAACAAGGTGGAAACAAGCTTCTTTATAGATGGCAACAAGTAGCACCAAATATTGATGAAAATCTCTTCATCAGAGTCTTGATTCAACCAGGCAATGGTAGTGTTCCTGGTCAAAGAACTGTAACTACTTCTTACCAAGCTCTATTTCTTGGTGGAGCTAATAGACTTCTCCAAGTTATGAAGCAGAGTTTTCCTGAATTGGGTTTAACAAGAAAGGATTGTTTGGAAACTAGTTGGATCAAATCGGTTCTTTATATCGCCGGTTATCCCAATACAACACCTCCAGAAGTTCTGCTCCAGGGAAAACCTACATCCAAGGCTTACTTCAAAGCAAAATCAGATTTTGTAAGACAAGTGATTCCAGAAACTTCCCTCAATTCCCTATGGAAAATTTTTCTTCAAGATGATGGTCCTTTAATGATATGGAATCCCTATGGAGGAATGATGAGTAGGATTGCAGAATCTGCAACACCTTTTCCTCACAGAGAAGGAACTCTTTACAAAATTCAGTACTTATCTGGATGGATTGATGGAGAAAAGAATATGGCAAAACATATGAGTTGGATGAGGAAATTTTACTCCTATATGACTCCTTATGCATCAAAAAATCCAAGGGAAAACTATGTGAATTATAGAGACTTGGATATAGGGATGAACAAGAAGAATTGTACAAGTCTTTCTCTTGCTAACTCTTGGGGTTATAGGTATTTTAAGGGTAACTTCAATAGGTTAGTAAAGGTGAAAACTAAAGTTGATCCATCTAATTTCTTTAGGCATGAGCAAAGTATCCCTCCACTTCCAATTGGTAAGTAA
Protein sequence

>MS.gene028346.t1

MQSMMSLLATFVVLLSVPLSTSVSIEEIFNNCLSLHAQIPNQFPSSIYTSTNKSYTSILESTAQNLRYLLPSVPKPDFIFTPFHDSQVQAAVICAKKHGIHMRIRSGGHDYEGLSYVSLIEKPFMILDLAKLRAVNVDIPHNTAWIQVGATIGEVYYKISAKSPVHGFPAGLCTSLGIGGLITGGAYGSMMRKYGLGVDNVVDAKIVNANGKILDRKAMGEDLFWAIRGGGGGSFGVILWWKIKLVPVPKTVTVFTVTKSLEQGGNKLLYRWQQVAPNIDENLFIRVLIQPGNGSVPGQRTVTTSYQALFLGGANRLLQVMKQSFPELGLTRKDCLETSWIKSVLYIAGYPNTTPPEVLLQGKPTSKAYFKAKSDFVRQVIPETSLNSLWKIFLQDDGPLMIWNPYGGMMSRIAESATPFPHREGTLYKIQYLSGWIDGEKNMAKHMSWMRKFYSYMTPYASKNPRENYVNYRDLDIGMNKKNCTSLSLANSWGYRYFKGNFNRLVKVKTKVDPSNFFRHEQSIPPLPIGK