AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28726

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28726.t1 MTR_4g094480 93.333 495 32 1 1 494 47 541 0.0 939
MS.gene28726.t1 MTR_4g094478 85.656 488 69 1 1 487 47 534 0.0 850
MS.gene28726.t1 MTR_4g094488 78.873 497 102 2 1 494 47 543 0.0 800
MS.gene28726.t1 MTR_4g094495 78.270 497 105 2 1 494 50 546 0.0 791
MS.gene28726.t1 MTR_4g094492 77.108 498 108 3 1 494 47 542 0.0 779
MS.gene28726.t1 MTR_4g094418 70.575 435 122 3 58 487 1 434 0.0 637
MS.gene28726.t1 MTR_2g031560 53.099 484 220 5 5 486 43 521 0.0 534
MS.gene28726.t1 MTR_4g094498 54.878 492 214 5 1 491 53 537 0.0 526
MS.gene28726.t1 MTR_2g031590 53.768 491 220 5 1 487 44 531 0.0 523
MS.gene28726.t1 MTR_2g031390 52.881 486 219 7 5 486 48 527 0.0 521
MS.gene28726.t1 MTR_2g031420 55.236 487 207 8 2 486 48 525 0.0 519
MS.gene28726.t1 MTR_3g009880 52.600 500 220 7 3 494 43 533 1.05e-180 517
MS.gene28726.t1 MTR_3g009820 53.053 475 214 5 13 486 54 520 1.88e-179 514
MS.gene28726.t1 MTR_3g009760 52.842 475 215 5 13 486 54 520 2.81e-179 513
MS.gene28726.t1 MTR_2g031460 54.433 485 212 6 2 485 48 524 5.56e-179 513
MS.gene28726.t1 MTR_4g094520 53.498 486 217 6 5 487 52 531 3.24e-176 506
MS.gene28726.t1 MTR_4g094518 53.183 487 218 7 4 486 48 528 9.92e-168 484
MS.gene28726.t1 MTR_4g088355 50.810 494 237 5 2 493 49 538 1.72e-167 484
MS.gene28726.t1 MTR_6g017205 50.305 491 238 4 1 489 41 527 9.18e-167 482
MS.gene28726.t1 MTR_2g031490 51.020 490 232 6 1 486 51 536 3.90e-166 481
MS.gene28726.t1 MTR_2g031510 51.837 490 228 6 1 486 53 538 1.54e-165 479
MS.gene28726.t1 MTR_4g091190 50.607 494 236 5 2 493 46 533 8.67e-164 474
MS.gene28726.t1 MTR_4g094515 52.439 492 229 4 1 491 666 1153 2.54e-163 493
MS.gene28726.t1 MTR_4g094515 51.626 492 229 6 1 488 54 540 1.26e-157 478
MS.gene28726.t1 MTR_4g091200 49.184 490 243 4 1 488 41 526 1.08e-162 471
MS.gene28726.t1 MTR_4g091150 50.820 488 234 5 2 486 54 538 2.79e-162 471
MS.gene28726.t1 MTR_4g057990 49.592 490 237 5 1 488 41 522 6.22e-161 466
MS.gene28726.t1 MTR_4g091170 47.425 466 223 5 27 491 101 545 2.11e-146 431
MS.gene28726.t1 MTR_2g023590 48.077 468 235 4 22 487 4 465 6.96e-144 421
MS.gene28726.t1 MTR_1g076830 43.983 482 256 7 11 485 58 532 4.15e-128 384
MS.gene28726.t1 MTR_3g009850 56.696 224 95 2 263 486 5 226 2.17e-83 258
MS.gene28726.t1 MTR_2g031600 60.500 200 78 1 1 199 45 244 1.80e-74 241
MS.gene28726.t1 MTR_2g031600 51.899 158 73 2 330 487 245 399 1.27e-50 178
MS.gene28726.t1 MTR_2g084370 43.636 110 41 2 82 191 1 89 3.05e-25 99.8
MS.gene28726.t1 MTR_2g084405 45.977 87 44 2 82 165 1 87 8.49e-16 73.6
MS.gene28726.t1 MTR_1g076760 83.333 42 6 1 214 254 33 74 1.08e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28726.t1 AT5G44440 51.020 490 225 6 3 486 47 527 2.32e-172 496
MS.gene28726.t1 AT5G44440 51.020 490 225 6 3 486 117 597 5.95e-171 495
MS.gene28726.t1 AT4G20820 50.308 487 228 5 1 486 51 524 1.86e-170 491
MS.gene28726.t1 AT5G44400 50.307 489 229 8 7 489 57 537 2.86e-165 478
MS.gene28726.t1 AT5G44410 50.407 492 223 8 2 486 50 527 5.05e-164 475
MS.gene28726.t1 AT2G34790 50.000 476 226 7 17 489 65 531 1.68e-161 468
MS.gene28726.t1 AT5G44380 51.318 493 226 8 1 486 52 537 2.90e-161 468
MS.gene28726.t1 AT5G44380 51.318 493 226 8 1 486 92 577 9.15e-161 468
MS.gene28726.t1 AT5G44360 51.271 472 212 9 21 486 69 528 1.59e-160 466
MS.gene28726.t1 AT5G44360 51.271 472 212 9 21 486 85 544 1.88e-160 466
MS.gene28726.t1 AT1G30760 49.275 483 231 7 11 489 61 533 3.28e-159 462
MS.gene28726.t1 AT1G30760 49.275 483 231 7 11 489 79 551 5.47e-159 462
MS.gene28726.t1 AT5G44390 48.589 496 239 9 1 487 51 539 7.85e-158 459
MS.gene28726.t1 AT1G30700 48.776 490 240 7 1 486 42 524 1.69e-153 448
MS.gene28726.t1 AT4G20860 50.214 468 216 10 23 486 73 527 6.08e-153 446
MS.gene28726.t1 AT4G20840 47.251 491 248 7 5 492 57 539 2.21e-152 445
MS.gene28726.t1 AT1G11770 47.551 490 246 7 1 487 47 528 3.03e-152 445
MS.gene28726.t1 AT1G30710 46.721 488 241 7 5 486 52 526 6.49e-152 444
MS.gene28726.t1 AT1G26410 49.036 467 230 6 21 486 88 547 1.19e-151 444
MS.gene28726.t1 AT1G34575 47.047 491 242 7 6 491 50 527 6.42e-151 441
MS.gene28726.t1 AT1G26420 47.708 480 240 8 9 486 53 523 5.40e-150 439
MS.gene28726.t1 AT1G26380 47.166 494 250 8 1 491 43 528 1.13e-148 436
MS.gene28726.t1 AT1G26390 47.752 467 238 4 21 486 64 525 5.57e-148 434
MS.gene28726.t1 AT1G30730 46.516 488 252 5 1 486 43 523 6.56e-148 433
MS.gene28726.t1 AT1G26400 46.327 490 247 8 1 485 43 521 1.24e-146 430
MS.gene28726.t1 AT1G30720 46.817 487 250 5 2 486 45 524 2.04e-144 424
MS.gene28726.t1 AT1G01980 46.293 499 252 9 1 491 47 537 2.22e-143 422
MS.gene28726.t1 AT4G20830 48.065 491 244 8 5 492 58 540 4.31e-143 422
MS.gene28726.t1 AT4G20830 48.148 486 241 8 5 487 58 535 5.40e-142 420
MS.gene28726.t1 AT4G20800 43.852 488 265 6 1 486 43 523 2.70e-140 414
MS.gene28726.t1 AT1G30740 46.247 493 250 8 1 487 44 527 1.25e-139 412
MS.gene28726.t1 AT1G30740 46.247 493 250 8 1 487 47 530 1.97e-139 412
MS.gene28726.t1 AT2G34810 43.558 489 270 4 2 486 51 537 2.85e-131 391

Find 101 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATGGTTGTTTGAATT+TGG 0.093676 4.3:-19468639 None:intergenic
GAATCGGTTCTTTACCTTTA+TGG 0.112377 4.3:+19469402 MS.gene28726:CDS
GTTATAAGCAAGCTAGTATT+TGG 0.173497 4.3:+19469847 MS.gene28726:CDS
AGAATTCCCATTTGTGAAAT+TGG 0.176513 4.3:+19469510 MS.gene28726:CDS
GAATCTGCAGGCCGTGGTTT+TGG 0.227134 4.3:-19468666 None:intergenic
AAAATACACCATTGAATCTT+TGG 0.242615 4.3:-19469571 None:intergenic
GATTATGGTTGTTTGAATTT+GGG 0.274542 4.3:-19468638 None:intergenic
CCTTTGCAAGATAACATAAA+TGG 0.276498 4.3:-19469223 None:intergenic
TTATTTCAAGCCTTGTTTCT+TGG 0.300694 4.3:+19469291 MS.gene28726:CDS
ATTTGTGAAATTGGATTAGA+AGG 0.307582 4.3:+19469519 MS.gene28726:CDS
TTCTAATCCAATTTCACAAA+TGG 0.318418 4.3:-19469517 None:intergenic
ATTCCATTTCCACATAGATA+TGG 0.327139 4.3:+19469636 MS.gene28726:CDS
CAATGGGTGAGGATTCCTTT+TGG 0.328365 4.3:+19469025 MS.gene28726:CDS
TAGAAAACTGTATAGTTATA+TGG 0.329156 4.3:+19469737 MS.gene28726:CDS
TCATTTCTGAAGAAGTTTAG+AGG 0.331220 4.3:-19469930 None:intergenic
AATTATAGAGATCTTGATAT+TGG 0.339773 4.3:+19469798 MS.gene28726:CDS
TTGAACACCGTCACACTTGA+TGG 0.340828 4.3:-19469117 None:intergenic
AATGAACCGAGTACAGAATT+TGG 0.344869 4.3:-19469668 None:intergenic
GGTGGTGGAGGAGCAAGTTT+TGG 0.349746 4.3:+19469057 MS.gene28726:CDS
CGAAGTCGTGGCCGCCGCTT+CGG 0.357017 4.3:-19468693 None:intergenic
TCTCTAACCAAACCAAGTTC+TGG 0.358087 4.3:-19469354 None:intergenic
TTATAAGCAAGCTAGTATTT+GGG 0.359669 4.3:+19469848 MS.gene28726:CDS
CGCCTTTCAGAAATCCATAA+AGG 0.368732 4.3:-19469416 None:intergenic
TATTGATGAGTAAGAGGAAT+TGG 0.375876 4.3:-19468533 None:intergenic
TGAATTTGGGAAATTTGTTT+AGG 0.377551 4.3:-19468625 None:intergenic
TTTCCATATCTATGTGGAAA+TGG 0.380986 4.3:-19469639 None:intergenic
CAAGAACAACTTCGAAAGAT+TGG 0.381018 4.3:+19469884 MS.gene28726:CDS
AGCCGGAAAAGCCGAAATCT+TGG 0.385793 4.3:+19468853 MS.gene28726:CDS
GGAAACTTGGTGCGTAAATT+CGG 0.389755 4.3:+19468934 MS.gene28726:CDS
AAGATTGTATTGAGATGAGT+TGG 0.395131 4.3:+19469376 MS.gene28726:CDS
AAATTCTGTACTCGGTTCAT+TGG 0.396326 4.3:+19469670 MS.gene28726:CDS
GTGAGATCAAAGATGACAAA+TGG 0.403756 4.3:-19468742 None:intergenic
GTGATAATGACAAGAGGTTT+TGG 0.406236 4.3:-19468601 None:intergenic
AGATAAACTCATTCCCTTGA+TGG 0.407746 4.3:+19469320 MS.gene28726:CDS
GGAATTCTATTTGTGTCCCT+TGG 0.420350 4.3:-19469976 None:intergenic
CGGCAGGAAAACCAAGATTT+CGG 0.423578 4.3:-19468864 None:intergenic
TCTAATCCAATTTCACAAAT+GGG 0.430583 4.3:-19469516 None:intergenic
GAAGTTTCCAGAACTTGGTT+TGG 0.432211 4.3:+19469347 MS.gene28726:CDS
TTTCAAGCCTTGTTTCTTGG+AGG 0.440275 4.3:+19469294 MS.gene28726:CDS
AGAACTACTTGGGTTCAATC+TGG 0.442862 4.3:+19468799 MS.gene28726:CDS
GAAGAGAAGTTTCCAGAACT+TGG 0.452732 4.3:+19469342 MS.gene28726:CDS
TGAGATGAGTTGGATTGAAT+CGG 0.456578 4.3:+19469386 MS.gene28726:CDS
CTTGGTGACTTCGAAACAAA+AGG 0.457842 4.3:-19469762 None:intergenic
CATTTATGTTATCTTGCAAA+GGG 0.465669 4.3:+19469224 MS.gene28726:CDS
TTGGAGTATTGATGAGTAAG+AGG 0.465775 4.3:-19468539 None:intergenic
TGGAAACTTCTCTTCCATCA+AGG 0.480434 4.3:-19469334 None:intergenic
AAGCGGCGGCCACGACTTCG+AGG 0.485100 4.3:+19468696 MS.gene28726:CDS
TGGGCTATTAGAGGTGGTGG+AGG 0.487997 4.3:+19469045 MS.gene28726:CDS
TGTCCTACTGTAGGTGTTGG+TGG 0.493627 4.3:+19468892 MS.gene28726:CDS
GGAAACTTCTCTTCCATCAA+GGG 0.502745 4.3:-19469333 None:intergenic
AACTTTACTACACAATTAGC+CGG 0.506150 4.3:+19468836 MS.gene28726:CDS
TTATCCATTATGCCACCATA+AGG 0.506968 4.3:-19469597 None:intergenic
TACCTTTATGGATTTCTGAA+AGG 0.509032 4.3:+19469414 MS.gene28726:CDS
ACCACCTCTAATAGCCCAAA+AGG 0.516876 4.3:-19469040 None:intergenic
CCATTTATGTTATCTTGCAA+AGG 0.517982 4.3:+19469223 MS.gene28726:CDS
GGACACAGTGATGGTGGCTA+TGG 0.518732 4.3:+19468913 MS.gene28726:CDS
TAATTGATATATGCAGCTCT+TGG 0.521563 4.3:-19469780 None:intergenic
TGAGATCAAAGATGACAAAT+GGG 0.525711 4.3:-19468741 None:intergenic
GGATTCGAATCTGCAGGCCG+TGG 0.528579 4.3:-19468672 None:intergenic
GGTAAAAGCGAAAACTAAAG+TGG 0.530638 4.3:+19469905 MS.gene28726:CDS
GTTTCTTGACAGAGAAGCAA+TGG 0.534469 4.3:+19469008 MS.gene28726:CDS
CGCTTCGGATTCGAATCTGC+AGG 0.534753 4.3:-19468678 None:intergenic
TTTATACCAAATTCTGTACT+CGG 0.539802 4.3:+19469662 MS.gene28726:CDS
TATAAATTTCCATATCTATG+TGG 0.540221 4.3:-19469645 None:intergenic
CAACAGAACACAAGCTGCAA+AGG 0.553522 4.3:+19469458 MS.gene28726:CDS
AAATGGGACTTCAGAAACAT+AGG 0.554207 4.3:-19468725 None:intergenic
TGATGGTGGCTATGGAAACT+TGG 0.555975 4.3:+19468921 MS.gene28726:CDS
TTTCTTGACAGAGAAGCAAT+GGG 0.565816 4.3:+19469009 MS.gene28726:CDS
TGGCGTATGTTTCATGAAGA+TGG 0.571964 4.3:+19469546 MS.gene28726:CDS
ACCTACAGTAGGACAAGCAC+CGG 0.573133 4.3:-19468884 None:intergenic
GTTCAATCTGGTGCAACACT+TGG 0.575161 4.3:+19468811 MS.gene28726:CDS
ACATAATTGATGTGAGGGGT+AGG 0.577701 4.3:+19468986 MS.gene28726:CDS
CAACAAAGCTTGTTCACAAG+TGG 0.578289 4.3:+19469166 MS.gene28726:CDS
TATACCTTCACATTTGTCCA+AGG 0.583193 4.3:+19469959 MS.gene28726:CDS
ACAGTAGGACAAGCACCGGC+AGG 0.585006 4.3:-19468880 None:intergenic
GCATCAAGAAAGTGATGAGG+TGG 0.587589 4.3:+19469692 MS.gene28726:CDS
ATCTACACCTCCAAGAAACA+AGG 0.588375 4.3:-19469301 None:intergenic
TGACAGAGAAGCAATGGGTG+AGG 0.588886 4.3:+19469014 MS.gene28726:CDS
TGCTCACATAATTGATGTGA+GGG 0.591207 4.3:+19468981 MS.gene28726:CDS
AGATGGTGCCAAAGATTCAA+TGG 0.596963 4.3:+19469563 MS.gene28726:CDS
GCCGGTGCTTGTCCTACTGT+AGG 0.597098 4.3:+19468883 MS.gene28726:CDS
GCTTGTCCTACTGTAGGTGT+TGG 0.601274 4.3:+19468889 MS.gene28726:CDS
AGGACCGACCCTCGAAGTCG+TGG 0.605427 4.3:-19468705 None:intergenic
ATTGGTGTGAATAATAACAA+TGG 0.605661 4.3:+19469816 MS.gene28726:CDS
GGTGTTGGTGGACACAGTGA+TGG 0.611521 4.3:+19468904 MS.gene28726:CDS
TATAAGCAAGCTAGTATTTG+GGG 0.615112 4.3:+19469849 MS.gene28726:CDS
AGCGGCGGCCACGACTTCGA+GGG 0.618854 4.3:+19468697 MS.gene28726:CDS
GCGGCCACGACTTCGAGGGT+CGG 0.627806 4.3:+19468701 MS.gene28726:CDS
ATACCTTCACATTTGTCCAA+GGG 0.635281 4.3:+19469960 MS.gene28726:CDS
TGAAGTTCCATCAAGTGTGA+CGG 0.645552 4.3:+19469110 MS.gene28726:CDS
CAGATTCGAATCCGAAGCGG+CGG 0.645614 4.3:+19468682 MS.gene28726:CDS
TTAGGTGTGATAATGACAAG+AGG 0.654557 4.3:-19468607 None:intergenic
TGTCCACCAACACCTACAGT+AGG 0.660735 4.3:-19468895 None:intergenic
GTTGGTGGACACAGTGATGG+TGG 0.665519 4.3:+19468907 MS.gene28726:CDS
TGTCCCTTGGACAAATGTGA+AGG 0.676388 4.3:-19469963 None:intergenic
CTGCAGATTCGAATCCGAAG+CGG 0.676489 4.3:+19468679 MS.gene28726:CDS
AAATTGGATTAGAAGGAATG+TGG 0.681300 4.3:+19469526 MS.gene28726:CDS
ATGCTCACATAATTGATGTG+AGG 0.682929 4.3:+19468980 MS.gene28726:CDS
ATAATCTGTTCCCAAAACCA+CGG 0.690765 4.3:+19468655 MS.gene28726:CDS
TTGGCATCAAGAAAGTGATG+AGG 0.701714 4.3:+19469689 MS.gene28726:CDS
GCTCACATAATTGATGTGAG+GGG 0.762973 4.3:+19468982 MS.gene28726:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGGAAAAAATTAAAATGAAT+TGG + chr4.3:19469712-19469731 MS.gene28726:CDS 15.0%
!! AATTATAGAGATCTTGATAT+TGG + chr4.3:19469798-19469817 MS.gene28726:CDS 20.0%
!! TAGAAAACTGTATAGTTATA+TGG + chr4.3:19469737-19469756 MS.gene28726:CDS 20.0%
!! TATAAATTTCCATATCTATG+TGG - chr4.3:19469648-19469667 None:intergenic 20.0%
! AAAATACACCATTGAATCTT+TGG - chr4.3:19469574-19469593 None:intergenic 25.0%
! AGATTATGGTTGTTTGAATT+TGG - chr4.3:19468642-19468661 None:intergenic 25.0%
! AGGGTAAATTCAAGTAAAAA+AGG + chr4.3:19469243-19469262 MS.gene28726:CDS 25.0%
! ATGTAGAAAAAAGAACTACT+TGG + chr4.3:19468788-19468807 MS.gene28726:CDS 25.0%
! ATTGGTGTGAATAATAACAA+TGG + chr4.3:19469816-19469835 MS.gene28726:CDS 25.0%
! CATTTATGTTATCTTGCAAA+GGG + chr4.3:19469224-19469243 MS.gene28726:CDS 25.0%
! GATTATGGTTGTTTGAATTT+GGG - chr4.3:19468641-19468660 None:intergenic 25.0%
! TCTAATCCAATTTCACAAAT+GGG - chr4.3:19469519-19469538 None:intergenic 25.0%
! TGTAGAAAAAAGAACTACTT+GGG + chr4.3:19468789-19468808 MS.gene28726:CDS 25.0%
! TTATAAGCAAGCTAGTATTT+GGG + chr4.3:19469848-19469867 MS.gene28726:CDS 25.0%
! TTCTAATCCAATTTCACAAA+TGG - chr4.3:19469520-19469539 None:intergenic 25.0%
! TTTATACCAAATTCTGTACT+CGG + chr4.3:19469662-19469681 MS.gene28726:CDS 25.0%
!! ATTTGTGAAATTGGATTAGA+AGG + chr4.3:19469519-19469538 MS.gene28726:CDS 25.0%
!! TGAATTTGGGAAATTTGTTT+AGG - chr4.3:19468628-19468647 None:intergenic 25.0%
!!! ATGGTGTATTTTTTTCCTTA+TGG + chr4.3:19469582-19469601 MS.gene28726:CDS 25.0%
AACTTTACTACACAATTAGC+CGG + chr4.3:19468836-19468855 MS.gene28726:CDS 30.0%
AAGATTGTATTGAGATGAGT+TGG + chr4.3:19469376-19469395 MS.gene28726:CDS 30.0%
AGAATTCCCATTTGTGAAAT+TGG + chr4.3:19469510-19469529 MS.gene28726:CDS 30.0%
ATTCCATTTCCACATAGATA+TGG + chr4.3:19469636-19469655 MS.gene28726:CDS 30.0%
CCATTTATGTTATCTTGCAA+AGG + chr4.3:19469223-19469242 MS.gene28726:CDS 30.0%
CCTTTGCAAGATAACATAAA+TGG - chr4.3:19469226-19469245 None:intergenic 30.0%
GTTATAAGCAAGCTAGTATT+TGG + chr4.3:19469847-19469866 MS.gene28726:CDS 30.0%
TAATTGATATATGCAGCTCT+TGG - chr4.3:19469783-19469802 None:intergenic 30.0%
TACCTTTATGGATTTCTGAA+AGG + chr4.3:19469414-19469433 MS.gene28726:CDS 30.0%
TATAAGCAAGCTAGTATTTG+GGG + chr4.3:19469849-19469868 MS.gene28726:CDS 30.0%
TATTGATGAGTAAGAGGAAT+TGG - chr4.3:19468536-19468555 None:intergenic 30.0%
TGAGATCAAAGATGACAAAT+GGG - chr4.3:19468744-19468763 None:intergenic 30.0%
TTTCCATATCTATGTGGAAA+TGG - chr4.3:19469642-19469661 None:intergenic 30.0%
! AAATTGGATTAGAAGGAATG+TGG + chr4.3:19469526-19469545 MS.gene28726:CDS 30.0%
! TCATTTCTGAAGAAGTTTAG+AGG - chr4.3:19469933-19469952 None:intergenic 30.0%
!! GCATTTTGATCCAATGTTTT+TGG - chr4.3:19469147-19469166 None:intergenic 30.0%
!! TGAGATTTTGAGTGGTAAAT+TGG - chr4.3:19468561-19468580 None:intergenic 30.0%
!! TTATTTCAAGCCTTGTTTCT+TGG + chr4.3:19469291-19469310 MS.gene28726:CDS 30.0%
!!! GTGTATTTTTTTCCTTATGG+TGG + chr4.3:19469585-19469604 MS.gene28726:CDS 30.0%
!!! GTTTTGGTGTCATAATTTCA+TGG + chr4.3:19469073-19469092 MS.gene28726:CDS 30.0%
AAATGGGACTTCAGAAACAT+AGG - chr4.3:19468728-19468747 None:intergenic 35.0%
AAATTCTGTACTCGGTTCAT+TGG + chr4.3:19469670-19469689 MS.gene28726:CDS 35.0%
AATGAACCGAGTACAGAATT+TGG - chr4.3:19469671-19469690 None:intergenic 35.0%
AGATAAACTCATTCCCTTGA+TGG + chr4.3:19469320-19469339 MS.gene28726:CDS 35.0%
ATAATCTGTTCCCAAAACCA+CGG + chr4.3:19468655-19468674 MS.gene28726:CDS 35.0%
ATACCTTCACATTTGTCCAA+GGG + chr4.3:19469960-19469979 MS.gene28726:CDS 35.0%
ATGCTCACATAATTGATGTG+AGG + chr4.3:19468980-19468999 MS.gene28726:CDS 35.0%
CAAGAACAACTTCGAAAGAT+TGG + chr4.3:19469884-19469903 MS.gene28726:CDS 35.0%
GGTAAAAGCGAAAACTAAAG+TGG + chr4.3:19469905-19469924 MS.gene28726:CDS 35.0%
GTGAGATCAAAGATGACAAA+TGG - chr4.3:19468745-19468764 None:intergenic 35.0%
GTTCAATGTGCCAAAAACAT+TGG + chr4.3:19469134-19469153 MS.gene28726:CDS 35.0%
TATACCTTCACATTTGTCCA+AGG + chr4.3:19469959-19469978 MS.gene28726:CDS 35.0%
TGCTCACATAATTGATGTGA+GGG + chr4.3:19468981-19469000 MS.gene28726:CDS 35.0%
TTATCCATTATGCCACCATA+AGG - chr4.3:19469600-19469619 None:intergenic 35.0%
TTTCTTGACAGAGAAGCAAT+GGG + chr4.3:19469009-19469028 MS.gene28726:CDS 35.0%
! GAATCGGTTCTTTACCTTTA+TGG + chr4.3:19469402-19469421 MS.gene28726:CDS 35.0%
! TGAGATGAGTTGGATTGAAT+CGG + chr4.3:19469386-19469405 MS.gene28726:CDS 35.0%
! TTAGGTGTGATAATGACAAG+AGG - chr4.3:19468610-19468629 None:intergenic 35.0%
! TTTTCCTTATGGTGGCATAA+TGG + chr4.3:19469593-19469612 MS.gene28726:CDS 35.0%
!! GTGATAATGACAAGAGGTTT+TGG - chr4.3:19468604-19468623 None:intergenic 35.0%
!! TGCAAATCTGAGATTTTGAG+TGG - chr4.3:19468569-19468588 None:intergenic 35.0%
!! TTGGAGTATTGATGAGTAAG+AGG - chr4.3:19468542-19468561 None:intergenic 35.0%
!!! AAGAGGTTTTGGTGTTGTTT+TGG - chr4.3:19468593-19468612 None:intergenic 35.0%
ACATAATTGATGTGAGGGGT+AGG + chr4.3:19468986-19469005 MS.gene28726:CDS 40.0%
AGAACTACTTGGGTTCAATC+TGG + chr4.3:19468799-19468818 MS.gene28726:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG - chr4.3:19469304-19469323 None:intergenic 40.0%
CGCCTTTCAGAAATCCATAA+AGG - chr4.3:19469419-19469438 None:intergenic 40.0%
GAAGAGAAGTTTCCAGAACT+TGG + chr4.3:19469342-19469361 MS.gene28726:CDS 40.0%
GAAGTTTCCAGAACTTGGTT+TGG + chr4.3:19469347-19469366 MS.gene28726:CDS 40.0%
GCTCACATAATTGATGTGAG+GGG + chr4.3:19468982-19469001 MS.gene28726:CDS 40.0%
GGAAACTTCTCTTCCATCAA+GGG - chr4.3:19469336-19469355 None:intergenic 40.0%
GTTTCTTGACAGAGAAGCAA+TGG + chr4.3:19469008-19469027 MS.gene28726:CDS 40.0%
TCTCTAACCAAACCAAGTTC+TGG - chr4.3:19469357-19469376 None:intergenic 40.0%
TGAAGTTCCATCAAGTGTGA+CGG + chr4.3:19469110-19469129 MS.gene28726:CDS 40.0%
TGGAAACTTCTCTTCCATCA+AGG - chr4.3:19469337-19469356 None:intergenic 40.0%
TGGCGTATGTTTCATGAAGA+TGG + chr4.3:19469546-19469565 MS.gene28726:CDS 40.0%
TTGGCATCAAGAAAGTGATG+AGG + chr4.3:19469689-19469708 MS.gene28726:CDS 40.0%
! AACCAAGATTTCGGCTTTTC+CGG - chr4.3:19468858-19468877 None:intergenic 40.0%
! AATGGGTGAGGATTCCTTTT+GGG + chr4.3:19469026-19469045 MS.gene28726:CDS 40.0%
! AGATGGTGCCAAAGATTCAA+TGG + chr4.3:19469563-19469582 MS.gene28726:CDS 40.0%
! CAACAAAGCTTGTTCACAAG+TGG + chr4.3:19469166-19469185 MS.gene28726:CDS 40.0%
! CTTGGTGACTTCGAAACAAA+AGG - chr4.3:19469765-19469784 None:intergenic 40.0%
! GATTCCTTTTGGGCTATTAG+AGG + chr4.3:19469036-19469055 MS.gene28726:CDS 40.0%
!! GGAAACTTGGTGCGTAAATT+CGG + chr4.3:19468934-19468953 MS.gene28726:CDS 40.0%
!! TTTCAAGCCTTGTTTCTTGG+AGG + chr4.3:19469294-19469313 MS.gene28726:CDS 40.0%
!!! GTGGTTTTGGGAACAGATTA+TGG - chr4.3:19468656-19468675 None:intergenic 40.0%
ACCACCTCTAATAGCCCAAA+AGG - chr4.3:19469043-19469062 None:intergenic 45.0%
CAACAGAACACAAGCTGCAA+AGG + chr4.3:19469458-19469477 MS.gene28726:CDS 45.0%
CGGCAGGAAAACCAAGATTT+CGG - chr4.3:19468867-19468886 None:intergenic 45.0%
GCATCAAGAAAGTGATGAGG+TGG + chr4.3:19469692-19469711 MS.gene28726:CDS 45.0%
GTTCAATCTGGTGCAACACT+TGG + chr4.3:19468811-19468830 MS.gene28726:CDS 45.0%
TGTCCCTTGGACAAATGTGA+AGG - chr4.3:19469966-19469985 None:intergenic 45.0%
TTGAACACCGTCACACTTGA+TGG - chr4.3:19469120-19469139 None:intergenic 45.0%
! CAATGGGTGAGGATTCCTTT+TGG + chr4.3:19469025-19469044 MS.gene28726:CDS 45.0%
! TCCTTTTGGGCTATTAGAGG+TGG + chr4.3:19469039-19469058 MS.gene28726:CDS 45.0%
! TTTTGGGCTATTAGAGGTGG+TGG + chr4.3:19469042-19469061 MS.gene28726:CDS 45.0%
!! CGAAATCTTGGTTTTCCTGC+CGG + chr4.3:19468865-19468884 MS.gene28726:CDS 45.0%
!! TGATGGTGGCTATGGAAACT+TGG + chr4.3:19468921-19468940 MS.gene28726:CDS 45.0%
ACCTACAGTAGGACAAGCAC+CGG - chr4.3:19468887-19468906 None:intergenic 50.0%
AGCCGGAAAAGCCGAAATCT+TGG + chr4.3:19468853-19468872 MS.gene28726:CDS 50.0%
CTGCAGATTCGAATCCGAAG+CGG + chr4.3:19468679-19468698 MS.gene28726:CDS 50.0%
GCTTGTCCTACTGTAGGTGT+TGG + chr4.3:19468889-19468908 MS.gene28726:CDS 50.0%
TGACAGAGAAGCAATGGGTG+AGG + chr4.3:19469014-19469033 MS.gene28726:CDS 50.0%
TGTCCACCAACACCTACAGT+AGG - chr4.3:19468898-19468917 None:intergenic 50.0%
! AATCTGCAGGCCGTGGTTTT+GGG - chr4.3:19468668-19468687 None:intergenic 50.0%
!! TGTCCTACTGTAGGTGTTGG+TGG + chr4.3:19468892-19468911 MS.gene28726:CDS 50.0%
CAGATTCGAATCCGAAGCGG+CGG + chr4.3:19468682-19468701 MS.gene28726:CDS 55.0%
CGCTTCGGATTCGAATCTGC+AGG - chr4.3:19468681-19468700 None:intergenic 55.0%
! GAATCTGCAGGCCGTGGTTT+TGG - chr4.3:19468669-19468688 None:intergenic 55.0%
! GTTGGTGGACACAGTGATGG+TGG + chr4.3:19468907-19468926 MS.gene28726:CDS 55.0%
! TGGGCTATTAGAGGTGGTGG+AGG + chr4.3:19469045-19469064 MS.gene28726:CDS 55.0%
!! GGACACAGTGATGGTGGCTA+TGG + chr4.3:19468913-19468932 MS.gene28726:CDS 55.0%
!! GGTGTTGGTGGACACAGTGA+TGG + chr4.3:19468904-19468923 MS.gene28726:CDS 55.0%
!!! GGTGGTGGAGGAGCAAGTTT+TGG + chr4.3:19469057-19469076 MS.gene28726:CDS 55.0%
GCCGGTGCTTGTCCTACTGT+AGG + chr4.3:19468883-19468902 MS.gene28726:CDS 60.0%
GGATTCGAATCTGCAGGCCG+TGG - chr4.3:19468675-19468694 None:intergenic 60.0%
!! ACAGTAGGACAAGCACCGGC+AGG - chr4.3:19468883-19468902 None:intergenic 60.0%
AGGACCGACCCTCGAAGTCG+TGG - chr4.3:19468708-19468727 None:intergenic 65.0%
AAGCGGCGGCCACGACTTCG+AGG + chr4.3:19468696-19468715 MS.gene28726:CDS 70.0%
AGCGGCGGCCACGACTTCGA+GGG + chr4.3:19468697-19468716 MS.gene28726:CDS 70.0%
CGAAGTCGTGGCCGCCGCTT+CGG - chr4.3:19468696-19468715 None:intergenic 70.0%
GCGGCCACGACTTCGAGGGT+CGG + chr4.3:19468701-19468720 MS.gene28726:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19468508 19469992 19468508 ID=MS.gene28726
chr4.3 mRNA 19468508 19469992 19468508 ID=MS.gene28726.t1;Parent=MS.gene28726
chr4.3 exon 19468508 19469992 19468508 ID=MS.gene28726.t1.exon1;Parent=MS.gene28726.t1
chr4.3 CDS 19468508 19469992 19468508 ID=cds.MS.gene28726.t1;Parent=MS.gene28726.t1
Gene Sequence

>MS.gene28726

ATGTCTAAAGTTGTGTACACAAAAACCAATTCCTCTTACTCATCAATACTCCAATTTACCACTCAAAATCTCAGATTTGCATCCAAAACAACACCAAAACCTCTTGTCATTATCACACCTAAACAAATTTCCCAAATTCAAACAACCATAATCTGTTCCCAAAACCACGGCCTGCAGATTCGAATCCGAAGCGGCGGCCACGACTTCGAGGGTCGGTCCTATGTTTCTGAAGTCCCATTTGTCATCTTTGATCTCACATACTTCAGAGAAATTACAGTACATGTAGAAAAAAGAACTACTTGGGTTCAATCTGGTGCAACACTTGGTGAACTTTACTACACAATTAGCCGGAAAAGCCGAAATCTTGGTTTTCCTGCCGGTGCTTGTCCTACTGTAGGTGTTGGTGGACACAGTGATGGTGGCTATGGAAACTTGGTGCGTAAATTCGGTCTTGCAGCTGATAATATAATTGATGCTCACATAATTGATGTGAGGGGTAGGTTTCTTGACAGAGAAGCAATGGGTGAGGATTCCTTTTGGGCTATTAGAGGTGGTGGAGGAGCAAGTTTTGGTGTCATAATTTCATGGAAGATTAAACTAGTTGAAGTTCCATCAAGTGTGACGGTGTTCAATGTGCCAAAAACATTGGATCAAAATGCAACAAAGCTTGTTCACAAGTGGCAGTTTGTGACAAGTACAATTGATGAAAACATAGCCATTTATGTTATCTTGCAAAGGGTAAATTCAAGTAAAAAAGGTAAGTCAAATTCAACAGTACAAGCTTTATTTCAAGCCTTGTTTCTTGGAGGTGTAGATAAACTCATTCCCTTGATGGAAGAGAAGTTTCCAGAACTTGGTTTGGTTAGAGAAGATTGTATTGAGATGAGTTGGATTGAATCGGTTCTTTACCTTTATGGATTTCTGAAAGGCGAATCACTTGAAATGTTGCTCAACAGAACACAAGCTGCAAAGGATATTTTCAAAATAAAGTCTGATTTTGTGAGAATTCCCATTTGTGAAATTGGATTAGAAGGAATGTGGCGTATGTTTCATGAAGATGGTGCCAAAGATTCAATGGTGTATTTTTTTCCTTATGGTGGCATAATGGATAATATTTCAGAGTCTGAAATTCCATTTCCACATAGATATGGAAATTTATACCAAATTCTGTACTCGGTTCATTGGCATCAAGAAAGTGATGAGGTGGAAAAAATTAAAATGAATTGGATTAGAAAACTGTATAGTTATATGGAACCTTTTGTTTCGAAGTCACCAAGAGCTGCATATATCAATTATAGAGATCTTGATATTGGTGTGAATAATAACAATGGATACACAAGTTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTCAAGAACAACTTCGAAAGATTGGTAAAAGCGAAAACTAAAGTGGATCCTCTAAACTTCTTCAGAAATGAACAGAGTATACCTTCACATTTGTCCAAGGGACACAAATAG
Protein sequence

>MS.gene28726.t1

MSKVVYTKTNSSYSSILQFTTQNLRFASKTTPKPLVIITPKQISQIQTTIICSQNHGLQIRIRSGGHDFEGRSYVSEVPFVIFDLTYFREITVHVEKRTTWVQSGATLGELYYTISRKSRNLGFPAGACPTVGVGGHSDGGYGNLVRKFGLAADNIIDAHIIDVRGRFLDREAMGEDSFWAIRGGGGASFGVIISWKIKLVEVPSSVTVFNVPKTLDQNATKLVHKWQFVTSTIDENIAIYVILQRVNSSKKGKSNSTVQALFQALFLGGVDKLIPLMEEKFPELGLVREDCIEMSWIESVLYLYGFLKGESLEMLLNRTQAAKDIFKIKSDFVRIPICEIGLEGMWRMFHEDGAKDSMVYFFPYGGIMDNISESEIPFPHRYGNLYQILYSVHWHQESDEVEKIKMNWIRKLYSYMEPFVSKSPRAAYINYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFERLVKAKTKVDPLNFFRNEQSIPSHLSKGHK