AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28742

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28742.t1 MTR_4g094418 95.259 464 22 0 103 566 1 464 0.0 920
MS.gene28742.t1 MTR_4g094488 71.165 541 145 7 1 536 2 536 0.0 761
MS.gene28742.t1 MTR_4g094478 69.444 540 156 5 1 537 2 535 0.0 754
MS.gene28742.t1 MTR_4g094495 69.301 544 153 5 1 536 2 539 0.0 749
MS.gene28742.t1 MTR_4g094480 68.901 537 158 6 3 536 4 534 0.0 738
MS.gene28742.t1 MTR_4g094492 67.276 547 167 6 1 542 2 541 0.0 737
MS.gene28742.t1 MTR_2g031560 52.319 539 248 5 7 545 2 531 0.0 585
MS.gene28742.t1 MTR_2g031590 53.789 541 237 7 7 542 5 537 0.0 572
MS.gene28742.t1 MTR_3g009820 53.271 535 236 7 5 538 2 523 0.0 566
MS.gene28742.t1 MTR_3g009880 53.962 530 232 6 9 538 4 521 0.0 561
MS.gene28742.t1 MTR_3g009760 53.084 535 237 7 5 538 2 523 0.0 561
MS.gene28742.t1 MTR_2g031390 52.250 511 233 6 29 537 28 529 0.0 559
MS.gene28742.t1 MTR_2g031460 53.959 543 237 7 7 548 8 538 0.0 557
MS.gene28742.t1 MTR_2g031420 53.659 533 234 7 7 538 8 528 0.0 551
MS.gene28742.t1 MTR_4g094498 56.719 506 208 5 33 538 41 535 0.0 550
MS.gene28742.t1 MTR_4g094520 50.000 538 257 7 2 537 5 532 0.0 526
MS.gene28742.t1 MTR_4g094518 52.427 515 228 7 28 537 28 530 7.25e-179 515
MS.gene28742.t1 MTR_4g094515 50.280 535 255 5 6 537 624 1150 1.48e-177 532
MS.gene28742.t1 MTR_4g094515 48.633 512 251 5 28 536 37 539 2.51e-163 495
MS.gene28742.t1 MTR_2g031490 48.315 534 268 5 6 537 11 538 2.78e-177 512
MS.gene28742.t1 MTR_2g031510 49.320 515 255 4 25 539 34 542 1.34e-174 505
MS.gene28742.t1 MTR_4g091150 50.097 513 243 7 29 537 37 540 1.09e-169 493
MS.gene28742.t1 MTR_4g091200 47.770 538 261 7 2 538 9 527 9.37e-167 484
MS.gene28742.t1 MTR_4g088355 49.318 513 248 5 29 538 31 534 1.08e-166 484
MS.gene28742.t1 MTR_6g017205 47.448 529 264 5 9 536 10 525 2.46e-164 479
MS.gene28742.t1 MTR_4g057990 47.249 527 263 6 13 538 11 523 1.61e-163 476
MS.gene28742.t1 MTR_4g091190 46.704 531 268 6 7 536 11 527 1.45e-162 474
MS.gene28742.t1 MTR_4g091170 47.761 469 219 5 70 536 97 541 3.92e-153 451
MS.gene28742.t1 MTR_2g023590 47.881 472 236 4 66 536 3 465 1.33e-148 436
MS.gene28742.t1 MTR_1g076830 44.294 517 269 9 30 538 31 536 1.36e-136 408
MS.gene28742.t1 MTR_3g009850 57.447 235 94 3 304 538 1 229 3.60e-89 275
MS.gene28742.t1 MTR_2g031600 57.500 240 100 2 7 245 6 244 1.07e-85 272
MS.gene28742.t1 MTR_2g031600 53.801 171 75 3 373 542 238 405 5.89e-55 191
MS.gene28742.t1 MTR_2g084370 46.847 111 37 2 127 237 1 89 1.03e-29 112
MS.gene28742.t1 MTR_2g084405 46.875 96 45 2 127 222 1 90 6.86e-18 79.7
MS.gene28742.t1 MTR_8g062280 35.354 99 34 3 436 534 4 72 3.65e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28742.t1 AT5G44440 49.724 543 257 6 1 539 71 601 0.0 538
MS.gene28742.t1 AT5G44440 49.724 543 257 6 1 539 1 531 0.0 538
MS.gene28742.t1 AT4G20820 50.278 539 249 7 2 538 6 527 0.0 536
MS.gene28742.t1 AT1G30760 50.195 512 240 6 28 536 50 549 3.99e-176 509
MS.gene28742.t1 AT1G30760 50.195 512 240 6 28 536 32 531 4.72e-176 508
MS.gene28742.t1 AT2G34790 49.153 531 249 9 12 536 14 529 6.67e-176 508
MS.gene28742.t1 AT5G44360 49.903 515 237 10 29 537 31 530 1.35e-175 507
MS.gene28742.t1 AT5G44360 49.903 515 237 10 29 537 47 546 1.95e-175 507
MS.gene28742.t1 AT5G44390 47.310 539 268 9 7 537 10 540 4.62e-175 506
MS.gene28742.t1 AT5G44400 47.349 547 267 10 1 537 1 536 2.02e-174 504
MS.gene28742.t1 AT5G44410 48.540 548 257 8 2 543 7 535 8.18e-174 503
MS.gene28742.t1 AT1G30700 49.533 535 256 7 6 537 3 526 4.08e-171 496
MS.gene28742.t1 AT4G20860 51.380 471 214 6 68 537 73 529 3.65e-169 491
MS.gene28742.t1 AT5G44380 48.244 541 259 10 7 537 10 539 1.90e-168 489
MS.gene28742.t1 AT5G44380 48.244 541 259 10 7 537 50 579 3.70e-168 490
MS.gene28742.t1 AT1G11770 48.039 510 256 6 28 537 29 529 7.69e-167 485
MS.gene28742.t1 AT4G20840 48.431 510 254 5 28 537 35 535 1.40e-166 484
MS.gene28742.t1 AT1G30710 46.741 537 265 8 8 538 8 529 1.79e-164 479
MS.gene28742.t1 AT1G34575 47.368 532 259 8 8 535 8 522 9.10e-161 469
MS.gene28742.t1 AT1G26420 48.218 533 257 12 10 537 7 525 9.72e-160 467
MS.gene28742.t1 AT1G01980 48.544 515 251 8 28 537 29 534 5.03e-159 465
MS.gene28742.t1 AT1G30730 45.094 530 280 5 7 535 4 523 8.58e-157 459
MS.gene28742.t1 AT4G20830 47.647 510 258 5 28 537 36 536 2.47e-156 458
MS.gene28742.t1 AT1G26380 47.059 527 267 8 12 537 10 525 5.93e-156 457
MS.gene28742.t1 AT4G20830 46.828 536 262 7 28 563 36 548 8.10e-156 458
MS.gene28742.t1 AT1G30740 46.082 536 276 8 6 537 5 531 2.40e-154 453
MS.gene28742.t1 AT1G30740 46.082 536 276 8 6 537 2 528 4.17e-154 452
MS.gene28742.t1 AT1G26390 46.746 507 262 4 31 537 29 527 1.03e-153 451
MS.gene28742.t1 AT1G26410 45.793 511 267 6 27 537 49 549 2.46e-153 451
MS.gene28742.t1 AT1G26400 44.653 533 282 8 3 534 1 521 3.25e-151 445
MS.gene28742.t1 AT1G30720 46.050 519 269 5 18 535 16 524 4.60e-151 444
MS.gene28742.t1 AT4G20800 45.419 513 268 7 27 538 25 526 1.38e-149 441
MS.gene28742.t1 AT2G34810 43.665 513 281 4 29 538 33 540 8.37e-146 431

Find 121 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAGTGGCTTGAATTTC+TGG 0.088778 4.3:+19653792 None:intergenic
CTTAAACATTAGGGTCATTT+TGG 0.158436 4.3:-19653208 MS.gene28742:CDS
GCTATAAGCAAGCTAGTATT+TGG 0.193317 4.3:-19652570 MS.gene28742:CDS
AGAGAGCCTATTCCTGATAT+TGG 0.262653 4.3:-19652907 MS.gene28742:CDS
AAGCAAAACTCTTGGCTTCC+TGG 0.271377 4.3:-19653568 MS.gene28742:CDS
TGACAATATTCAGAGTTTCT+AGG 0.284906 4.3:-19653299 MS.gene28742:CDS
CTGAATATTGTCACAGTTGA+TGG 0.291976 4.3:+19653309 None:intergenic
ACATTTGAATCCTTGTTTCT+TGG 0.300277 4.3:-19653138 MS.gene28742:CDS
ATTCCTGATATTGGGTTAGA+AGG 0.305269 4.3:-19652898 MS.gene28742:CDS
TTGTTTCTTGGAGGTGTAGA+TGG 0.315456 4.3:-19653126 MS.gene28742:CDS
CAATGGGTGAGGATCTGTTT+TGG 0.324567 4.3:-19653401 MS.gene28742:CDS
TGGAATAATGGATGAGATTT+CGG 0.325191 4.3:-19652812 MS.gene28742:CDS
ATACCTTCTCTGTCTCGTTT+TGG 0.346393 4.3:-19652457 MS.gene28742:CDS
CACTCCTTATGGTGGAATAA+TGG 0.346906 4.3:-19652824 MS.gene28742:CDS
GATGCGTAAGTTTGGTCTTT+CGG 0.350217 4.3:-19653484 MS.gene28742:CDS
TAAGAATAACTTCAAAAGAT+TGG 0.350745 4.3:-19652533 MS.gene28742:CDS
TTGTGTTGGATGTGAATCTA+AGG 0.355973 4.3:+19653853 None:intergenic
AACCCTTCTAACCCAATATC+AGG 0.364075 4.3:+19652895 None:intergenic
TTGATGAGGACTTAAACATT+AGG 0.365884 4.3:-19653218 MS.gene28742:CDS
TATGATTAGAATAACTTTCA+AGG 0.367516 4.3:+19653955 None:intergenic
CTATAAGCAAGCTAGTATTT+GGG 0.368691 4.3:-19652569 MS.gene28742:CDS
GGAGGTGGAGGTTCAAGCTT+TGG 0.369864 4.3:-19653369 MS.gene28742:CDS
CTTATCAGTGGCGGCGGCTA+TGG 0.371457 4.3:-19653513 MS.gene28742:CDS
GAGAGCCTATTCCTGATATT+GGG 0.380379 4.3:-19652906 MS.gene28742:CDS
TTTGAATCCTTGTTTCTTGG+AGG 0.404634 4.3:-19653135 MS.gene28742:CDS
ATATTGGGTTAGAAGGGTTA+TGG 0.406546 4.3:-19652891 MS.gene28742:CDS
CATCTACTTGGACTTCTTGA+AGG 0.407407 4.3:+19653661 None:intergenic
ATGAGTATGAGGAGTGTGTT+TGG 0.409800 4.3:+19653901 None:intergenic
CTTGGAGACTTTGACACAAA+AGG 0.412025 4.3:+19652655 None:intergenic
TGATGAGGACTTAAACATTA+GGG 0.412260 4.3:-19653217 MS.gene28742:CDS
ATGAGATCAATGACAACAAA+TGG 0.413992 4.3:+19653687 None:intergenic
GTGTTGATATTCACTCCTTA+TGG 0.417599 4.3:-19652835 MS.gene28742:CDS
TCTAACCCAATATCAGGAAT+AGG 0.418514 4.3:+19652901 None:intergenic
GGATTCTTGATGCGTAAGTT+TGG 0.420162 4.3:-19653492 MS.gene28742:CDS
TTCTATTCAACAAAACTTCA+AGG 0.420531 4.3:+19652974 None:intergenic
AATTATAGAGATCTTGATAT+TGG 0.441086 4.3:-19652619 MS.gene28742:CDS
AAGATTGTACTGAGATGAGT+TGG 0.447941 4.3:-19653053 MS.gene28742:CDS
GTTCAAGGAGGGGCAACTGC+TGG 0.456565 4.3:-19653618 MS.gene28742:CDS
AATCTGCATGTCATGGCGTT+GGG 0.459044 4.3:+19653764 None:intergenic
GGTTCGTGACAGAGAATCAA+TGG 0.464766 4.3:-19653418 MS.gene28742:CDS
TGCCCTACTGTAGGTGTTAG+CGG 0.465359 4.3:-19653537 MS.gene28742:CDS
AATAGCAGTGCATGGGTTCA+AGG 0.466266 4.3:-19653633 MS.gene28742:CDS
TAATTCACATATGCACCTCT+TGG 0.471919 4.3:+19652637 None:intergenic
AATACATTGCCTTGTGAAGA+AGG 0.472389 4.3:-19652415 MS.gene28742:CDS
TCTCTAACCAAACCAAGCTC+TGG 0.473432 4.3:+19653075 None:intergenic
GGAGTTTCCAGAGCTTGGTT+TGG 0.476037 4.3:-19653082 MS.gene28742:CDS
TAGAAAACTTTATAGTTACA+TGG 0.480388 4.3:-19652680 MS.gene28742:CDS
TGGAAACTCCTCTTCCATCA+AGG 0.482075 4.3:+19653095 None:intergenic
GAAGAGGAGTTTCCAGAGCT+TGG 0.483846 4.3:-19653087 MS.gene28742:CDS
ATTCAGAGTTTCTAGGACAC+TGG 0.484970 4.3:-19653292 MS.gene28742:CDS
AGATGGACTTATCCCCTTGA+TGG 0.488351 4.3:-19653109 MS.gene28742:CDS
GAATCTGCATGTCATGGCGT+TGG 0.498652 4.3:+19653763 None:intergenic
TCATCCATTATTCCACCATA+AGG 0.498834 4.3:+19652820 None:intergenic
AGCAACAACAGTTAAATATG+AGG 0.499237 4.3:+19654046 None:intergenic
CCGGAGTATAGATGAGTATG+AGG 0.500286 4.3:+19653890 None:intergenic
TTCCTGATATTGGGTTAGAA+GGG 0.500382 4.3:-19652897 MS.gene28742:CDS
TGGGCTATTAGAGGAGGTGG+AGG 0.501388 4.3:-19653381 MS.gene28742:CDS
TTGGAAAGAGGAAGGTGATG+AGG 0.502301 4.3:-19652728 MS.gene28742:CDS
ACATAATTGATGTGAATGGT+AGG 0.505623 4.3:-19653440 MS.gene28742:CDS
AAAACTCTTGGCTTCCTGGC+AGG 0.506023 4.3:-19653564 MS.gene28742:CDS
ATTCCATTCCCACACAGAGC+TGG 0.515576 4.3:-19652781 MS.gene28742:CDS
TGAGTATGAGGAGTGTGTTT+GGG 0.516907 4.3:+19653902 None:intergenic
TCTATTCAACAAAACTTCAA+GGG 0.517551 4.3:+19652975 None:intergenic
TTTCCAGCTCTGTGTGGGAA+TGG 0.526072 4.3:+19652778 None:intergenic
CACAAACTTTGAGATTGAAG+TGG 0.530763 4.3:+19653929 None:intergenic
AAATGGAACTTCAGAAACAT+AGG 0.532136 4.3:+19653704 None:intergenic
CTGCCACTTGTGAATAAGCC+TGG 0.540705 4.3:+19653257 None:intergenic
TGAATTTCTGGTATTTCAAG+AGG 0.544410 4.3:+19653804 None:intergenic
GGAAACTCCTCTTCCATCAA+GGG 0.545602 4.3:+19653096 None:intergenic
CGTAGTCGTGGCCACCACTT+CGG 0.548447 4.3:+19653736 None:intergenic
ATGTAGAAAATAGCAGTGCA+TGG 0.560180 4.3:-19653641 MS.gene28742:CDS
GCAGGTATTTGCCCTACTGT+AGG 0.561317 4.3:-19653546 MS.gene28742:CDS
AATTGCATCCCTTCTTCACA+AGG 0.562431 4.3:+19652406 None:intergenic
CATAATTGATGTGAATGGTA+GGG 0.562579 4.3:-19653439 MS.gene28742:CDS
CAGTAGGGCAAATACCTGCC+AGG 0.563841 4.3:+19653550 None:intergenic
TTGATATTCACTCCTTATGG+TGG 0.565905 4.3:-19652832 MS.gene28742:CDS
ATGCAATTATTAGAGTTCCC+TGG 0.575808 4.3:-19652391 MS.gene28742:CDS
GCTTTGGAGTTATCATAGCA+TGG 0.581899 4.3:-19653353 MS.gene28742:CDS
GCAGTGCATGGGTTCAAGGA+GGG 0.582300 4.3:-19653629 MS.gene28742:CDS
ACTTATCCCCTTGATGGAAG+AGG 0.583355 4.3:-19653103 MS.gene28742:CDS
AGTCCGCTAACACCTACAGT+AGG 0.585729 4.3:+19653534 None:intergenic
TTGTTGAATAGAACTCATAG+TGG 0.587672 4.3:-19652964 MS.gene28742:CDS
TGACAGAGAATCAATGGGTG+AGG 0.587800 4.3:-19653412 MS.gene28742:CDS
CTATTCAACAAAACTTCAAG+GGG 0.590722 4.3:+19652976 None:intergenic
AGCGGACTTATCAGTGGCGG+CGG 0.591156 4.3:-19653519 MS.gene28742:CDS
CAAGAGGTGTCACAATGACA+AGG 0.593405 4.3:+19653820 None:intergenic
TGTAGAAAATAGCAGTGCAT+GGG 0.602666 4.3:-19653640 MS.gene28742:CDS
ATTGGTGTGAATAATAACAA+TGG 0.605661 4.3:-19652601 MS.gene28742:CDS
ATACATTGCCTTGTGAAGAA+GGG 0.605745 4.3:-19652414 MS.gene28742:CDS
GTTCGTGACAGAGAATCAAT+GGG 0.608043 4.3:-19653417 MS.gene28742:CDS
AGGACAGACCTTCGTAGTCG+TGG 0.610475 4.3:+19653724 None:intergenic
GCTCACATAATTGATGTGAA+TGG 0.610798 4.3:-19653444 MS.gene28742:CDS
CAGTGCATGGGTTCAAGGAG+GGG 0.614481 4.3:-19653628 MS.gene28742:CDS
CACTGGAACAAAATGCAACC+AGG 0.614754 4.3:-19653275 MS.gene28742:CDS
ATCTACACCTCCAAGAAACA+AGG 0.617374 4.3:+19653128 None:intergenic
ATTGAATCAGTTCTCTACCA+TGG 0.621990 4.3:-19653030 MS.gene28742:CDS
AAGAAACAAGGATTCAAATG+TGG 0.623440 4.3:+19653140 None:intergenic
GGATTCGAATCTGCATGTCA+TGG 0.624027 4.3:+19653757 None:intergenic
TATAAGCAAGCTAGTATTTG+GGG 0.624423 4.3:-19652568 MS.gene28742:CDS
AAGAGGTGTCACAATGACAA+GGG 0.626627 4.3:+19653821 None:intergenic
GTCCGCTAACACCTACAGTA+GGG 0.627164 4.3:+19653535 None:intergenic
CAGATTCGAATCCGAAGTGG+TGG 0.628502 4.3:-19653747 MS.gene28742:CDS
CAACCAGGCTTATTCACAAG+TGG 0.642716 4.3:-19653260 MS.gene28742:CDS
AAATGTTTCCAGCTCTGTGT+GGG 0.643042 4.3:+19652773 None:intergenic
CCTCATACTCATCTATACTC+CGG 0.648391 4.3:-19653890 MS.gene28742:CDS
GGTGTTAGCGGACTTATCAG+TGG 0.652652 4.3:-19653525 MS.gene28742:CDS
AGAGGTGTCACAATGACAAG+GGG 0.652727 4.3:+19653822 None:intergenic
GAATCAGTTCTCTACCATGG+TGG 0.653386 4.3:-19653027 MS.gene28742:CDS
AGTAGCAAATAAGCTTGATG+AGG 0.653614 4.3:-19653232 MS.gene28742:CDS
TTACCAAAACGAGACAGAGA+AGG 0.660634 4.3:+19652454 None:intergenic
AGCAGTGCATGGGTTCAAGG+AGG 0.665984 4.3:-19653630 MS.gene28742:CDS
GAAACTCCTCTTCCATCAAG+GGG 0.668982 4.3:+19653097 None:intergenic
ATGCAGATTCGAATCCGAAG+TGG 0.674707 4.3:-19653750 MS.gene28742:CDS
TTTGTGTCAAAGTCTCCAAG+AGG 0.676435 4.3:-19652652 MS.gene28742:CDS
TAAATGTTTCCAGCTCTGTG+TGG 0.685122 4.3:+19652772 None:intergenic
TGAAAACGAGGCTAAATCTG+CGG 0.694314 4.3:-19652857 MS.gene28742:CDS
AGTGGTGGCCACGACTACGA+AGG 0.696564 4.3:-19653732 MS.gene28742:CDS
GTTAGCGGACTTATCAGTGG+CGG 0.699597 4.3:-19653522 MS.gene28742:CDS
TGAATACTAGTACTCAAACA+AGG 0.702644 4.3:-19653179 MS.gene28742:CDS
AATCACTTGTAAAACCACCA+TGG 0.721311 4.3:+19653013 None:intergenic
GCGTTGGGAACAGATTACAG+TGG 0.741001 4.3:+19653779 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAAAAAAGGTATATAAAT+TGG - chr4.3:19653723-19653742 MS.gene28742:CDS 15.0%
!! AATTATAGAGATCTTGATAT+TGG - chr4.3:19653809-19653828 MS.gene28742:CDS 20.0%
!! TAAGAATAACTTCAAAAGAT+TGG - chr4.3:19653895-19653914 MS.gene28742:CDS 20.0%
!! TAGAAAACTTTATAGTTACA+TGG - chr4.3:19653748-19653767 MS.gene28742:CDS 20.0%
!! TATGATTAGAATAACTTTCA+AGG + chr4.3:19652476-19652495 None:intergenic 20.0%
! ATTGGTGTGAATAATAACAA+TGG - chr4.3:19653827-19653846 MS.gene28742:CDS 25.0%
! TCTATTCAACAAAACTTCAA+GGG + chr4.3:19653456-19653475 None:intergenic 25.0%
! TTCTATTCAACAAAACTTCA+AGG + chr4.3:19653457-19653476 None:intergenic 25.0%
!! TCATTTCTGAAAAAGTTTAG+AGG + chr4.3:19653944-19653963 None:intergenic 25.0%
!!! AAAAAACTAAGTGCTGAATT+TGG + chr4.3:19653682-19653701 None:intergenic 25.0%
!!! AAATTCAGCACTTAGTTTTT+TGG - chr4.3:19653681-19653700 MS.gene28742:CDS 25.0%
!!! GTTTTGGTAACATAACAATA+AGG - chr4.3:19653987-19654006 MS.gene28742:CDS 25.0%
AAATGGAACTTCAGAAACAT+AGG + chr4.3:19652727-19652746 None:intergenic 30.0%
AAGAAACAAGGATTCAAATG+TGG + chr4.3:19653291-19653310 None:intergenic 30.0%
ACATAATTGATGTGAATGGT+AGG - chr4.3:19652988-19653007 MS.gene28742:CDS 30.0%
AGCAACAACAGTTAAATATG+AGG + chr4.3:19652385-19652404 None:intergenic 30.0%
ATGAGATCAATGACAACAAA+TGG + chr4.3:19652744-19652763 None:intergenic 30.0%
CATAATTGATGTGAATGGTA+GGG - chr4.3:19652989-19653008 MS.gene28742:CDS 30.0%
CTATAAGCAAGCTAGTATTT+GGG - chr4.3:19653859-19653878 MS.gene28742:CDS 30.0%
CTATTCAACAAAACTTCAAG+GGG + chr4.3:19653455-19653474 None:intergenic 30.0%
TATAAGCAAGCTAGTATTTG+GGG - chr4.3:19653860-19653879 MS.gene28742:CDS 30.0%
TGAATACTAGTACTCAAACA+AGG - chr4.3:19653249-19653268 MS.gene28742:CDS 30.0%
TGACAATATTCAGAGTTTCT+AGG - chr4.3:19653129-19653148 MS.gene28742:CDS 30.0%
TGATGAGGACTTAAACATTA+GGG - chr4.3:19653211-19653230 MS.gene28742:CDS 30.0%
TTGATGAGGACTTAAACATT+AGG - chr4.3:19653210-19653229 MS.gene28742:CDS 30.0%
TTGTTGAATAGAACTCATAG+TGG - chr4.3:19653464-19653483 MS.gene28742:CDS 30.0%
! ACATTTGAATCCTTGTTTCT+TGG - chr4.3:19653290-19653309 MS.gene28742:CDS 30.0%
! CTTAAACATTAGGGTCATTT+TGG - chr4.3:19653220-19653239 MS.gene28742:CDS 30.0%
!! ATTTTATATGTAGAGCACCA+GGG + chr4.3:19654057-19654076 None:intergenic 30.0%
!! TATTTTATATGTAGAGCACC+AGG + chr4.3:19654058-19654077 None:intergenic 30.0%
!! TGAATTTCTGGTATTTCAAG+AGG + chr4.3:19652627-19652646 None:intergenic 30.0%
!! TGGAATAATGGATGAGATTT+CGG - chr4.3:19653616-19653635 MS.gene28742:CDS 30.0%
!!! TAAGGTTTTGAACAGAAAAC+CGG + chr4.3:19652560-19652579 None:intergenic 30.0%
!!! TGCTATTTTCTACATCTACT+TGG + chr4.3:19652782-19652801 None:intergenic 30.0%
!!! TTAGTTTTTTGGAAAGAGGA+AGG - chr4.3:19653692-19653711 MS.gene28742:CDS 30.0%
AAGATTGTACTGAGATGAGT+TGG - chr4.3:19653375-19653394 MS.gene28742:CDS 35.0%
AAGGTGATGAGGTAGAAAAA+AGG - chr4.3:19653711-19653730 MS.gene28742:CDS 35.0%
AATACATTGCCTTGTGAAGA+AGG - chr4.3:19654013-19654032 MS.gene28742:CDS 35.0%
AATCACTTGTAAAACCACCA+TGG + chr4.3:19653418-19653437 None:intergenic 35.0%
AGCCAAAAAAGCAAAACTCT+TGG - chr4.3:19652852-19652871 MS.gene28742:CDS 35.0%
AGTAGCAAATAAGCTTGATG+AGG - chr4.3:19653196-19653215 MS.gene28742:CDS 35.0%
ATACATTGCCTTGTGAAGAA+GGG - chr4.3:19654014-19654033 MS.gene28742:CDS 35.0%
ATGCAATTATTAGAGTTCCC+TGG - chr4.3:19654037-19654056 MS.gene28742:CDS 35.0%
ATGTAGAAAATAGCAGTGCA+TGG - chr4.3:19652787-19652806 MS.gene28742:CDS 35.0%
ATTACAGTGGCTTGAATTTC+TGG + chr4.3:19652639-19652658 None:intergenic 35.0%
ATTGAATCAGTTCTCTACCA+TGG - chr4.3:19653398-19653417 MS.gene28742:CDS 35.0%
CTGAATATTGTCACAGTTGA+TGG + chr4.3:19653122-19653141 None:intergenic 35.0%
GCTATAAGCAAGCTAGTATT+TGG - chr4.3:19653858-19653877 MS.gene28742:CDS 35.0%
GCTCACATAATTGATGTGAA+TGG - chr4.3:19652984-19653003 MS.gene28742:CDS 35.0%
TAATTCACATATGCACCTCT+TGG + chr4.3:19653794-19653813 None:intergenic 35.0%
TCATCCATTATTCCACCATA+AGG + chr4.3:19653611-19653630 None:intergenic 35.0%
TCTAACCCAATATCAGGAAT+AGG + chr4.3:19653530-19653549 None:intergenic 35.0%
TGTAGAAAATAGCAGTGCAT+GGG - chr4.3:19652788-19652807 MS.gene28742:CDS 35.0%
TTGATATTCACTCCTTATGG+TGG - chr4.3:19653596-19653615 MS.gene28742:CDS 35.0%
TTGTGTTGGATGTGAATCTA+AGG + chr4.3:19652578-19652597 None:intergenic 35.0%
! ATATTGGGTTAGAAGGGTTA+TGG - chr4.3:19653537-19653556 MS.gene28742:CDS 35.0%
! ATTCCTGATATTGGGTTAGA+AGG - chr4.3:19653530-19653549 MS.gene28742:CDS 35.0%
! GCCAATGTTTTATGAAAACG+AGG - chr4.3:19653559-19653578 MS.gene28742:CDS 35.0%
! GCCTCGTTTTCATAAAACAT+TGG + chr4.3:19653563-19653582 None:intergenic 35.0%
! GTGTTGATATTCACTCCTTA+TGG - chr4.3:19653593-19653612 MS.gene28742:CDS 35.0%
! TTCCTGATATTGGGTTAGAA+GGG - chr4.3:19653531-19653550 MS.gene28742:CDS 35.0%
! TTTGAATCCTTGTTTCTTGG+AGG - chr4.3:19653293-19653312 MS.gene28742:CDS 35.0%
!! CACAAACTTTGAGATTGAAG+TGG + chr4.3:19652502-19652521 None:intergenic 35.0%
!!! AGCCAAGAGTTTTGCTTTTT+TGG + chr4.3:19652857-19652876 None:intergenic 35.0%
!!! GCACTTAGTTTTTTGGAAAG+AGG - chr4.3:19653688-19653707 MS.gene28742:CDS 35.0%
!!! TAGTGGTGTGTTGTTTTTCA+AGG - chr4.3:19653481-19653500 MS.gene28742:CDS 35.0%
AAATGTTTCCAGCTCTGTGT+GGG + chr4.3:19653658-19653677 None:intergenic 40.0%
AACCCTTCTAACCCAATATC+AGG + chr4.3:19653536-19653555 None:intergenic 40.0%
AATTGCATCCCTTCTTCACA+AGG + chr4.3:19654025-19654044 None:intergenic 40.0%
AGAGAGCCTATTCCTGATAT+TGG - chr4.3:19653521-19653540 MS.gene28742:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG + chr4.3:19653303-19653322 None:intergenic 40.0%
CATCTACTTGGACTTCTTGA+AGG + chr4.3:19652770-19652789 None:intergenic 40.0%
CCTCATACTCATCTATACTC+CGG - chr4.3:19652538-19652557 MS.gene28742:CDS 40.0%
CTTGGAGACTTTGACACAAA+AGG + chr4.3:19653776-19653795 None:intergenic 40.0%
GAGAGCCTATTCCTGATATT+GGG - chr4.3:19653522-19653541 MS.gene28742:CDS 40.0%
GGATTCTTGATGCGTAAGTT+TGG - chr4.3:19652936-19652955 MS.gene28742:CDS 40.0%
GTTCGTGACAGAGAATCAAT+GGG - chr4.3:19653011-19653030 MS.gene28742:CDS 40.0%
TAAATGTTTCCAGCTCTGTG+TGG + chr4.3:19653659-19653678 None:intergenic 40.0%
TGAAAACGAGGCTAAATCTG+CGG - chr4.3:19653571-19653590 MS.gene28742:CDS 40.0%
TTACCAAAACGAGACAGAGA+AGG + chr4.3:19653977-19653996 None:intergenic 40.0%
TTTGTGTCAAAGTCTCCAAG+AGG - chr4.3:19653776-19653795 MS.gene28742:CDS 40.0%
! AATGGGTGAGGATCTGTTTT+GGG - chr4.3:19653028-19653047 MS.gene28742:CDS 40.0%
! ATACCTTCTCTGTCTCGTTT+TGG - chr4.3:19653971-19653990 MS.gene28742:CDS 40.0%
! ATGAGTATGAGGAGTGTGTT+TGG + chr4.3:19652530-19652549 None:intergenic 40.0%
! CACTCCTTATGGTGGAATAA+TGG - chr4.3:19653604-19653623 MS.gene28742:CDS 40.0%
! GATGCGTAAGTTTGGTCTTT+CGG - chr4.3:19652944-19652963 MS.gene28742:CDS 40.0%
! TGAGTATGAGGAGTGTGTTT+GGG + chr4.3:19652529-19652548 None:intergenic 40.0%
! TTGTTTCTTGGAGGTGTAGA+TGG - chr4.3:19653302-19653321 MS.gene28742:CDS 40.0%
!! AAGAGGTGTCACAATGACAA+GGG + chr4.3:19652610-19652629 None:intergenic 40.0%
!! ACATTAGGGTCATTTTGGAG+AGG - chr4.3:19653225-19653244 MS.gene28742:CDS 40.0%
!! ATTCAGAGTTTCTAGGACAC+TGG - chr4.3:19653136-19653155 MS.gene28742:CDS 40.0%
!! CATTAGGGTCATTTTGGAGA+GGG - chr4.3:19653226-19653245 MS.gene28742:CDS 40.0%
!! GATCTGTTTTGGGCTATTAG+AGG - chr4.3:19653038-19653057 MS.gene28742:CDS 40.0%
!! GCTTTGGAGTTATCATAGCA+TGG - chr4.3:19653075-19653094 MS.gene28742:CDS 40.0%
AAGCAAAACTCTTGGCTTCC+TGG - chr4.3:19652860-19652879 MS.gene28742:CDS 45.0%
AATAGCAGTGCATGGGTTCA+AGG - chr4.3:19652795-19652814 MS.gene28742:CDS 45.0%
AATCTGCATGTCATGGCGTT+GGG + chr4.3:19652667-19652686 None:intergenic 45.0%
ACTTATCCCCTTGATGGAAG+AGG - chr4.3:19653325-19653344 MS.gene28742:CDS 45.0%
ATGCAGATTCGAATCCGAAG+TGG - chr4.3:19652678-19652697 MS.gene28742:CDS 45.0%
CAACCAGGCTTATTCACAAG+TGG - chr4.3:19653168-19653187 MS.gene28742:CDS 45.0%
CACTGGAACAAAATGCAACC+AGG - chr4.3:19653153-19653172 MS.gene28742:CDS 45.0%
CCGGAGTATAGATGAGTATG+AGG + chr4.3:19652541-19652560 None:intergenic 45.0%
GAAACTCCTCTTCCATCAAG+GGG + chr4.3:19653334-19653353 None:intergenic 45.0%
GAATCAGTTCTCTACCATGG+TGG - chr4.3:19653401-19653420 MS.gene28742:CDS 45.0%
GGAAACTCCTCTTCCATCAA+GGG + chr4.3:19653335-19653354 None:intergenic 45.0%
GGATTCGAATCTGCATGTCA+TGG + chr4.3:19652674-19652693 None:intergenic 45.0%
GGTTCGTGACAGAGAATCAA+TGG - chr4.3:19653010-19653029 MS.gene28742:CDS 45.0%
TCTCTAACCAAACCAAGCTC+TGG + chr4.3:19653356-19653375 None:intergenic 45.0%
TGACAGAGAATCAATGGGTG+AGG - chr4.3:19653016-19653035 MS.gene28742:CDS 45.0%
TGGAAACTCCTCTTCCATCA+AGG + chr4.3:19653336-19653355 None:intergenic 45.0%
TTGGAAAGAGGAAGGTGATG+AGG - chr4.3:19653700-19653719 MS.gene28742:CDS 45.0%
! AAGGGGTTTTGCAGTTGTGT+TGG + chr4.3:19652592-19652611 None:intergenic 45.0%
! AGATGGACTTATCCCCTTGA+TGG - chr4.3:19653319-19653338 MS.gene28742:CDS 45.0%
! CAATGGGTGAGGATCTGTTT+TGG - chr4.3:19653027-19653046 MS.gene28742:CDS 45.0%
! TTTTGGGCTATTAGAGGAGG+TGG - chr4.3:19653044-19653063 MS.gene28742:CDS 45.0%
!! AGAGGTGTCACAATGACAAG+GGG + chr4.3:19652609-19652628 None:intergenic 45.0%
!! CAAGAGGTGTCACAATGACA+AGG + chr4.3:19652611-19652630 None:intergenic 45.0%
!! CTGTTTTGGGCTATTAGAGG+AGG - chr4.3:19653041-19653060 MS.gene28742:CDS 45.0%
AAAACTCTTGGCTTCCTGGC+AGG - chr4.3:19652864-19652883 MS.gene28742:CDS 50.0%
AGTCCGCTAACACCTACAGT+AGG + chr4.3:19652897-19652916 None:intergenic 50.0%
ATTCCATTCCCACACAGAGC+TGG - chr4.3:19653647-19653666 MS.gene28742:CDS 50.0%
CAGATTCGAATCCGAAGTGG+TGG - chr4.3:19652681-19652700 MS.gene28742:CDS 50.0%
GAAGAGGAGTTTCCAGAGCT+TGG - chr4.3:19653341-19653360 MS.gene28742:CDS 50.0%
GAATCTGCATGTCATGGCGT+TGG + chr4.3:19652668-19652687 None:intergenic 50.0%
GCAGGTATTTGCCCTACTGT+AGG - chr4.3:19652882-19652901 MS.gene28742:CDS 50.0%
GCGTTGGGAACAGATTACAG+TGG + chr4.3:19652652-19652671 None:intergenic 50.0%
GGAGTTTCCAGAGCTTGGTT+TGG - chr4.3:19653346-19653365 MS.gene28742:CDS 50.0%
GTCCGCTAACACCTACAGTA+GGG + chr4.3:19652896-19652915 None:intergenic 50.0%
TGCCCTACTGTAGGTGTTAG+CGG - chr4.3:19652891-19652910 MS.gene28742:CDS 50.0%
TTTCCAGCTCTGTGTGGGAA+TGG + chr4.3:19653653-19653672 None:intergenic 50.0%
! CTGCCACTTGTGAATAAGCC+TGG + chr4.3:19653174-19653193 None:intergenic 50.0%
!! GGTGTTAGCGGACTTATCAG+TGG - chr4.3:19652903-19652922 MS.gene28742:CDS 50.0%
!! GTTAGCGGACTTATCAGTGG+CGG - chr4.3:19652906-19652925 MS.gene28742:CDS 50.0%
AGCAGTGCATGGGTTCAAGG+AGG - chr4.3:19652798-19652817 MS.gene28742:CDS 55.0%
AGGACAGACCTTCGTAGTCG+TGG + chr4.3:19652707-19652726 None:intergenic 55.0%
CAGTAGGGCAAATACCTGCC+AGG + chr4.3:19652881-19652900 None:intergenic 55.0%
CAGTGCATGGGTTCAAGGAG+GGG - chr4.3:19652800-19652819 MS.gene28742:CDS 55.0%
GCAGTGCATGGGTTCAAGGA+GGG - chr4.3:19652799-19652818 MS.gene28742:CDS 55.0%
GGAGGTGGAGGTTCAAGCTT+TGG - chr4.3:19653059-19653078 MS.gene28742:CDS 55.0%
TGGGCTATTAGAGGAGGTGG+AGG - chr4.3:19653047-19653066 MS.gene28742:CDS 55.0%
AGTGGTGGCCACGACTACGA+AGG - chr4.3:19652696-19652715 MS.gene28742:CDS 60.0%
CGTAGTCGTGGCCACCACTT+CGG + chr4.3:19652695-19652714 None:intergenic 60.0%
CTTATCAGTGGCGGCGGCTA+TGG - chr4.3:19652915-19652934 MS.gene28742:CDS 60.0%
GTTCAAGGAGGGGCAACTGC+TGG - chr4.3:19652810-19652829 MS.gene28742:CDS 60.0%
! AGCGGACTTATCAGTGGCGG+CGG - chr4.3:19652909-19652928 MS.gene28742:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19652372 19654078 19652372 ID=MS.gene28742
chr4.3 mRNA 19652372 19654078 19652372 ID=MS.gene28742.t1;Parent=MS.gene28742
chr4.3 exon 19652372 19654078 19652372 ID=MS.gene28742.t1.exon1;Parent=MS.gene28742.t1
chr4.3 CDS 19652372 19654078 19652372 ID=cds.MS.gene28742.t1;Parent=MS.gene28742.t1
Gene Sequence

>MS.gene28742

ATGCTTCTAACCTCATATTTAACTGTTGTTGCTATTATTGCTTTGTTATTTTCTTTTACATCTTATGCTGCTGATAATAATCATGAAAGCTTCATTCAATGCCTTGAAAGTTATTCTAATCATAATTCCACTTCAATCTCAAAGTTTGTGTACACCCAAACACACTCCTCATACTCATCTATACTCCGGTTTTCTGTTCAAAACCTTAGATTCACATCCAACACAACTGCAAAACCCCTTGTCATTGTGACACCTCTTGAAATACCAGAAATTCAAGCCACTGTAATCTGTTCCCAACGCCATGACATGCAGATTCGAATCCGAAGTGGTGGCCACGACTACGAAGGTCTGTCCTATGTTTCTGAAGTTCCATTTGTTGTCATTGATCTCATAAACCTTCAAGAAGTCCAAGTAGATGTAGAAAATAGCAGTGCATGGGTTCAAGGAGGGGCAACTGCTGGCGAACTTTTGTACAAAATTAGCCAAAAAAGCAAAACTCTTGGCTTCCTGGCAGGTATTTGCCCTACTGTAGGTGTTAGCGGACTTATCAGTGGCGGCGGCTATGGATTCTTGATGCGTAAGTTTGGTCTTTCGGCTGATAATATACTTGACGCTCACATAATTGATGTGAATGGTAGGGTTCGTGACAGAGAATCAATGGGTGAGGATCTGTTTTGGGCTATTAGAGGAGGTGGAGGTTCAAGCTTTGGAGTTATCATAGCATGGAAGATAAAACTAGTTCGTGTTCCATCAACTGTGACAATATTCAGAGTTTCTAGGACACTGGAACAAAATGCAACCAGGCTTATTCACAAGTGGCAGTTAGTAGCAAATAAGCTTGATGAGGACTTAAACATTAGGGTCATTTTGGAGAGGGTGAATACTAGTACTCAAACAAGGAAACTAACAATAAAAGCCACATTTGAATCCTTGTTTCTTGGAGGTGTAGATGGACTTATCCCCTTGATGGAAGAGGAGTTTCCAGAGCTTGGTTTGGTTAGAGAAGATTGTACTGAGATGAGTTGGATTGAATCAGTTCTCTACCATGGTGGTTTTACAAGTGATTTTACAAGTGATCAACCCCTTGAAGTTTTGTTGAATAGAACTCATAGTGGTGTGTTGTTTTTCAAGGCAAAATCTGATTATGTGAGAGAGCCTATTCCTGATATTGGGTTAGAAGGGTTATGGCCAATGTTTTATGAAAACGAGGCTAAATCTGCGGTGTTGATATTCACTCCTTATGGTGGAATAATGGATGAGATTTCGGAGTCTGAAATTCCATTCCCACACAGAGCTGGAAACATTTACCAAATTCAGCACTTAGTTTTTTGGAAAGAGGAAGGTGATGAGGTAGAAAAAAGGTATATAAATTGGATTAGAAAACTTTATAGTTACATGGAACCTTTTGTGTCAAAGTCTCCAAGAGGTGCATATGTGAATTATAGAGATCTTGATATTGGTGTGAATAATAACAATGGATACACAAGCTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTTAAGAATAACTTCAAAAGATTGGCAAAAGTGAAGACAAAAGTTGATCCTCTAAACTTTTTCAGAAATGAACAGAGTATACCTTCTCTGTCTCGTTTTGGTAACATAACAATAAGGCATAATACATTGCCTTGTGAAGAAGGGATGCAATTATTAGAGTTCCCTGGTGCTCTACATATAAAATAA
Protein sequence

>MS.gene28742.t1

MLLTSYLTVVAIIALLFSFTSYAADNNHESFIQCLESYSNHNSTSISKFVYTQTHSSYSSILRFSVQNLRFTSNTTAKPLVIVTPLEIPEIQATVICSQRHDMQIRIRSGGHDYEGLSYVSEVPFVVIDLINLQEVQVDVENSSAWVQGGATAGELLYKISQKSKTLGFLAGICPTVGVSGLISGGGYGFLMRKFGLSADNILDAHIIDVNGRVRDRESMGEDLFWAIRGGGGSSFGVIIAWKIKLVRVPSTVTIFRVSRTLEQNATRLIHKWQLVANKLDEDLNIRVILERVNTSTQTRKLTIKATFESLFLGGVDGLIPLMEEEFPELGLVREDCTEMSWIESVLYHGGFTSDFTSDQPLEVLLNRTHSGVLFFKAKSDYVREPIPDIGLEGLWPMFYENEAKSAVLIFTPYGGIMDEISESEIPFPHRAGNIYQIQHLVFWKEEGDEVEKRYINWIRKLYSYMEPFVSKSPRGAYVNYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSLSRFGNITIRHNTLPCEEGMQLLEFPGALHIK