AlfalfaGEDB | MS.gene28725

M. sativa cultivar XinJiangDaYe / MS.gene28725

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0036094 GO:0043167 GO:0043168 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071949 GO:0097159 GO:1901265 GO:1901363
PFAM: PF01565.26 PF08031.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28725.t1	XP_013457290.1	97	440	13	0	1	440	104	543	6.90E-252	879.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28725.t1	O64743	54.3	438	185	7	1	435	106	531	5.6e-141	502.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28725.t1	G8A1B3	97.0	440	13	0	1	440	104	543	5.0e-252	879.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050381	MS.gene28725	0.813468	2.52E-51	-1.69E-46
MS.gene050883	MS.gene28725	0.805105	1.57E-49	-1.69E-46
MS.gene051044	MS.gene28725	0.831899	1.29E-55	-1.69E-46
MS.gene051138	MS.gene28725	0.826602	2.48E-54	-1.69E-46
MS.gene052802	MS.gene28725	0.808547	2.94E-50	-1.69E-46
MS.gene054056	MS.gene28725	0.802492	5.47E-49	-1.69E-46
MS.gene055124	MS.gene28725	0.829199	5.88E-55	-1.69E-46
MS.gene055942	MS.gene28725	0.827248	1.74E-54	-1.69E-46
MS.gene058099	MS.gene28725	0.825948	3.55E-54	-1.69E-46
MS.gene058171	MS.gene28725	0.835941	1.25E-56	-1.69E-46
MS.gene058173	MS.gene28725	0.822217	2.67E-53	-1.69E-46
MS.gene058175	MS.gene28725	0.831796	1.36E-55	-1.69E-46
MS.gene059045	MS.gene28725	0.843312	1.51E-58	-1.69E-46
MS.gene059690	MS.gene28725	0.800848	1.19E-48	-1.69E-46
MS.gene059694	MS.gene28725	0.808457	3.07E-50	-1.69E-46
MS.gene059697	MS.gene28725	0.817815	2.71E-52	-1.69E-46
MS.gene061044	MS.gene28725	0.824693	7.03E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene003357	MS.gene28725	PPI
MS.gene28724	MS.gene28725	PPI
MS.gene51644	MS.gene28725	PPI
MS.gene009481	MS.gene28725	PPI
MS.gene28725	MS.gene028353	PPI
MS.gene028357	MS.gene28725	PPI
MS.gene28725	MS.gene51643	PPI
MS.gene35114	MS.gene28725	PPI
MS.gene28725	MS.gene51646	PPI
MS.gene28725	MS.gene028354	PPI
MS.gene35113	MS.gene28725	PPI
MS.gene36421	MS.gene28725	PPI
MS.gene28725	MS.gene35103	PPI
MS.gene28725	MS.gene36437	PPI
MS.gene28726	MS.gene28725	PPI
MS.gene35083	MS.gene28725	PPI
MS.gene28717	MS.gene28725	PPI
MS.gene28725	MS.gene56278	PPI
MS.gene28725	MS.gene028346	PPI
MS.gene28723	MS.gene28725	PPI
MS.gene28725	MS.gene63535	PPI
MS.gene028352	MS.gene28725	PPI
MS.gene35102	MS.gene28725	PPI
MS.gene28725	MS.gene36435	PPI
MS.gene63532	MS.gene28725	PPI
MS.gene28742	MS.gene28725	PPI
MS.gene36445	MS.gene28725	PPI
MS.gene36439	MS.gene28725	PPI
MS.gene009479	MS.gene28725	PPI
MS.gene35104	MS.gene28725	PPI
MS.gene56279	MS.gene28725	PPI
MS.gene028369	MS.gene28725	PPI
MS.gene028347	MS.gene28725	PPI
MS.gene35106	MS.gene28725	PPI
MS.gene02146	MS.gene28725	PPI
MS.gene28727	MS.gene28725	PPI
MS.gene28725	MS.gene56282	PPI
MS.gene28725	MS.gene047977	PPI
MS.gene35105	MS.gene28725	PPI
MS.gene63533	MS.gene28725	PPI
MS.gene28725	MS.gene36444	PPI
MS.gene36438	MS.gene28725	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28725.t1	MTR_4g094488	97.045	440	13	0	1	440	104	543	0.0	890
MS.gene28725.t1	MTR_4g094495	83.636	440	72	0	1	440	107	546	0.0	753
MS.gene28725.t1	MTR_4g094478	81.293	433	79	1	1	433	104	534	0.0	726
MS.gene28725.t1	MTR_4g094492	79.910	443	82	3	1	440	104	542	0.0	715
MS.gene28725.t1	MTR_4g094480	79.545	440	88	1	1	440	104	541	0.0	713
MS.gene28725.t1	MTR_4g094418	73.227	437	110	2	1	433	1	434	0.0	667
MS.gene28725.t1	MTR_3g009820	59.722	432	165	3	1	432	98	520	0.0	528
MS.gene28725.t1	MTR_3g009880	60.185	432	163	3	1	432	96	518	0.0	527
MS.gene28725.t1	MTR_3g009760	59.815	433	163	4	1	432	98	520	0.0	526
MS.gene28725.t1	MTR_2g031390	55.862	435	181	6	1	432	101	527	7.26e-178	508
MS.gene28725.t1	MTR_2g031560	56.713	432	181	3	1	432	96	521	1.31e-177	508
MS.gene28725.t1	MTR_2g031420	59.722	432	164	6	1	432	104	525	9.90e-173	494
MS.gene28725.t1	MTR_2g031460	59.629	431	164	5	1	431	104	524	6.13e-171	490
MS.gene28725.t1	MTR_2g031590	56.452	434	185	3	1	433	101	531	1.12e-169	487
MS.gene28725.t1	MTR_4g094518	57.931	435	172	6	1	432	102	528	1.28e-167	482
MS.gene28725.t1	MTR_4g094520	57.241	435	176	6	1	433	105	531	2.09e-167	481
MS.gene28725.t1	MTR_4g094498	56.064	437	183	3	1	437	110	537	5.29e-166	478
MS.gene28725.t1	MTR_2g031490	54.943	435	187	4	1	432	108	536	3.24e-156	453
MS.gene28725.t1	MTR_2g031510	54.023	435	191	4	1	432	110	538	1.85e-154	449
MS.gene28725.t1	MTR_4g094515	55.149	437	190	3	1	437	723	1153	1.36e-149	454
MS.gene28725.t1	MTR_4g094515	53.547	437	193	5	1	434	111	540	9.19e-143	437
MS.gene28725.t1	MTR_4g057990	53.103	435	194	4	1	434	97	522	2.64e-149	435
MS.gene28725.t1	MTR_4g088355	52.392	439	202	4	1	438	105	537	4.99e-149	434
MS.gene28725.t1	MTR_4g091150	53.456	434	195	5	1	432	110	538	3.64e-148	432
MS.gene28725.t1	MTR_6g017205	53.318	437	195	4	1	435	98	527	3.97e-148	432
MS.gene28725.t1	MTR_4g091200	52.523	436	198	4	1	434	98	526	5.67e-147	429
MS.gene28725.t1	MTR_4g091170	49.545	440	197	5	1	439	132	547	7.22e-145	425
MS.gene28725.t1	MTR_4g091190	51.259	437	204	3	2	437	103	531	1.47e-144	423
MS.gene28725.t1	MTR_2g023590	49.078	434	212	3	1	433	40	465	1.11e-135	398
MS.gene28725.t1	MTR_1g076830	44.977	438	224	6	1	431	105	532	1.58e-117	354
MS.gene28725.t1	MTR_3g009850	58.667	225	89	3	209	432	5	226	3.42e-88	268
MS.gene28725.t1	MTR_2g031600	73.427	143	38	0	1	143	102	244	2.03e-62	207
MS.gene28725.t1	MTR_2g031600	52.532	158	72	2	276	433	245	399	1.39e-51	179
MS.gene28725.t1	MTR_2g084370	48.649	111	35	2	25	135	1	89	1.83e-32	119
MS.gene28725.t1	MTR_2g084405	58.750	80	33	0	25	104	1	80	4.29e-24	96.7
MS.gene28725.t1	MTR_8g062280	36.893	103	35	3	333	435	4	76	1.13e-12	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28725.t1	AT2G34790	54.338	438	185	7	1	435	106	531	6.45e-165	475
MS.gene28725.t1	AT5G44400	54.005	437	186	7	1	432	108	534	1.26e-163	472
MS.gene28725.t1	AT4G20820	56.019	432	175	5	1	432	108	524	7.56e-162	467
MS.gene28725.t1	AT5G44360	55.963	436	173	9	1	432	108	528	4.36e-161	465
MS.gene28725.t1	AT5G44360	55.963	436	173	9	1	432	124	544	5.74e-161	465
MS.gene28725.t1	AT1G30760	53.881	438	187	6	1	435	108	533	1.30e-160	464
MS.gene28725.t1	AT1G30760	53.881	438	187	6	1	435	126	551	1.82e-160	464
MS.gene28725.t1	AT5G44440	54.233	437	184	5	1	432	102	527	2.60e-160	463
MS.gene28725.t1	AT4G20860	55.046	436	179	9	1	432	105	527	2.22e-159	461
MS.gene28725.t1	AT5G44440	54.233	437	184	5	1	432	172	597	2.66e-159	463
MS.gene28725.t1	AT5G44390	52.273	440	193	9	1	433	110	539	1.04e-153	447
MS.gene28725.t1	AT5G44410	53.653	438	181	7	1	432	106	527	1.03e-152	444
MS.gene28725.t1	AT5G44380	54.587	436	185	6	1	432	111	537	6.61e-151	439
MS.gene28725.t1	AT5G44380	54.587	436	185	6	1	432	151	577	1.40e-150	440
MS.gene28725.t1	AT1G26410	53.118	433	192	7	1	432	125	547	6.38e-148	432
MS.gene28725.t1	AT1G30710	52.174	437	189	8	1	432	105	526	1.23e-147	431
MS.gene28725.t1	AT1G26390	53.241	432	195	4	1	432	101	525	6.00e-147	429
MS.gene28725.t1	AT1G26380	52.727	440	199	6	1	440	101	531	2.13e-146	428
MS.gene28725.t1	AT1G26420	52.656	433	194	7	1	432	101	523	6.41e-146	426
MS.gene28725.t1	AT1G11770	51.494	435	199	5	1	433	104	528	3.67e-145	425
MS.gene28725.t1	AT1G34575	51.247	441	196	8	1	437	102	527	1.71e-144	422
MS.gene28725.t1	AT1G30730	51.270	433	201	5	1	432	100	523	7.10e-144	421
MS.gene28725.t1	AT1G30700	52.414	435	195	6	1	432	99	524	1.06e-143	421
MS.gene28725.t1	AT4G20840	50.685	438	204	5	3	438	112	539	1.61e-143	421
MS.gene28725.t1	AT1G26400	51.376	436	201	6	1	435	101	526	4.80e-141	414
MS.gene28725.t1	AT1G30720	51.039	433	202	5	1	432	101	524	2.26e-138	407
MS.gene28725.t1	AT4G20800	50.691	434	202	6	1	432	100	523	5.80e-137	404
MS.gene28725.t1	AT4G20830	49.886	439	206	6	3	438	113	540	5.19e-134	396
MS.gene28725.t1	AT1G01980	50.113	443	204	7	2	437	105	537	1.41e-133	395
MS.gene28725.t1	AT4G20830	50.000	434	203	6	3	433	113	535	1.37e-132	394
MS.gene28725.t1	AT1G30740	49.545	440	202	7	1	433	104	530	1.32e-130	387
MS.gene28725.t1	AT1G30740	49.545	440	202	7	1	433	101	527	1.60e-130	387
MS.gene28725.t1	AT2G34810	47.126	435	223	4	1	432	107	537	1.44e-126	377

Find 90 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTATAAGCAAGCTAGTATT+TGG	0.193317	4.3:-19454129	MS.gene28725:CDS
AATCCTTCTAAACCAGTTTC+AGG	0.221499	4.3:+19454454	None:intergenic
GTTTCAAGTGGTTCGCCTTC+TGG	0.231675	4.3:+19454547	None:intergenic
TAGAAAACTTTATAGTTATA+TGG	0.249330	4.3:-19454239	MS.gene28725:CDS
CAATGGGTGAAGATTTGTTT+TGG	0.263104	4.3:-19454957	MS.gene28725:CDS
TTATTTCAATCCTTGTTTCT+AGG	0.269621	4.3:-19454685	MS.gene28725:CDS
CTAATGGTATTGTTTCCTTA+TGG	0.282122	4.3:-19454394	MS.gene28725:CDS
AAACTGGTTTAGAAGGATTA+TGG	0.294935	4.3:-19454450	MS.gene28725:CDS
AATTATAGAGATCTTGATAT+TGG	0.300885	4.3:-19454178	MS.gene28725:CDS
CGCCTTCTGGAAACCGAAAA+AGG	0.314622	4.3:+19454560	None:intergenic
ATTCCTGAAACTGGTTTAGA+AGG	0.316827	4.3:-19454457	MS.gene28725:CDS
CACTTCAGTGGTGGTGGATA+TGG	0.317127	4.3:-19455069	MS.gene28725:CDS
TCTAAACCAGTTTCAGGAAT+AGG	0.319812	4.3:+19454460	None:intergenic
AAAATTCCTATTCCTGAAAC+TGG	0.323303	4.3:-19454466	MS.gene28725:CDS
TCATTTCTGAAGAAGTTTAG+AGG	0.331220	4.3:+19454046	None:intergenic
ATTGGTGTGAATAACATTAA+TGG	0.335537	4.3:-19454160	MS.gene28725:CDS
ATTCCATTTCCACATAGATA+TGG	0.343229	4.3:-19454340	MS.gene28725:CDS
GGTGGTGGAGGAGCAAGTTT+TGG	0.349746	4.3:-19454925	MS.gene28725:CDS
GTAAACACTGTTACAGTTGA+TGG	0.351158	4.3:+19454865	None:intergenic
CTATAAGCAAGCTAGTATTT+GGG	0.368691	4.3:-19454128	MS.gene28725:CDS
TGGAGAAGTTGCACATCAAT+TGG	0.370174	4.3:-19454264	MS.gene28725:CDS
CTTGGAGACTTTGAAACAAA+AGG	0.375011	4.3:+19454214	None:intergenic
CAAGAACAACTTCAAAAGAT+TGG	0.382060	4.3:-19454092	MS.gene28725:CDS
GTTTCCTTATGGTGGCATAA+TGG	0.388858	4.3:-19454383	MS.gene28725:CDS
GAAGAAAAGTTTCCTGAACT+TGG	0.392423	4.3:-19454634	MS.gene28725:CDS
GTTCCATATCTATGTGGAAA+TGG	0.394166	4.3:+19454337	None:intergenic
GAAATTCTATTTGCGTCCCT+TGG	0.394677	4.3:+19454000	None:intergenic
AGATAACCTCTTACCCTTGA+TGG	0.394714	4.3:-19454656	MS.gene28725:CDS
TCTCTAACCAACCCAAGTTC+AGG	0.400856	4.3:+19454622	None:intergenic
GACAAAATCAGATTTCGCTT+TGG	0.411110	4.3:+19454489	None:intergenic
GTGAGCATCAATGATGTTAT+CGG	0.419983	4.3:+19455017	None:intergenic
AAAACTCTTGGTTTCCCTGC+AGG	0.420339	4.3:-19455120	MS.gene28725:CDS
TTTCTTCCATCAAGGGTAAG+AGG	0.424421	4.3:+19454650	None:intergenic
AAAACTGCTTGGGTTCAATC+TGG	0.435993	4.3:-19455186	MS.gene28725:CDS
AAAGCTAGTACTGTTGACTT+TGG	0.458454	4.3:+19454706	None:intergenic
TAATTAATATATGCAGCTCT+TGG	0.463039	4.3:+19454196	None:intergenic
GGAACCTTGTTGCGTAAATA+CGG	0.466163	4.3:-19455048	MS.gene28725:CDS
AAGATTGTGTTGAAATGAGT+TGG	0.466692	4.3:-19454600	MS.gene28725:CDS
GTGAGATCAATGATGACAAA+AGG	0.482657	4.3:+19455243	None:intergenic
TGGGCTATTAGAGGTGGTGG+AGG	0.490480	4.3:-19454937	MS.gene28725:CDS
TGTAGAAAACAAAACTGCTT+GGG	0.493208	4.3:-19455196	MS.gene28725:CDS
TGGTGCGGAAGAGGTACTAA+TGG	0.495510	4.3:-19454410	MS.gene28725:CDS
TAGCGGTGGCCACGACTTCG+AGG	0.497341	4.3:-19455289	MS.gene28725:CDS
AAAGTTTCCTGAACTTGGGT+TGG	0.502529	4.3:-19454629	MS.gene28725:CDS
TTATCCATTATGCCACCATA+AGG	0.506968	4.3:+19454379	None:intergenic
GAAGTTTAGAGGATCAACTT+TGG	0.511187	4.3:+19454057	None:intergenic
AAGAAAAGTTTCCTGAACTT+GGG	0.512048	4.3:-19454633	MS.gene28725:CDS
TGAAATGAGTTGGATTGAAT+CGG	0.514164	4.3:-19454590	MS.gene28725:CDS
ACAGTAGGACAAACACCTGC+AGG	0.514616	4.3:+19455105	None:intergenic
ATGGTATTGTTTCCTTATGG+TGG	0.522116	4.3:-19454391	MS.gene28725:CDS
TGGCCTATGTTTGATGAAGA+TGG	0.529049	4.3:-19454430	MS.gene28725:CDS
CTGTTGAGCAACGTTTCAAG+TGG	0.530743	4.3:+19454535	None:intergenic
TGTCCTACTGTAGGTGTTGG+TGG	0.531106	4.3:-19455093	MS.gene28725:CDS
GTTTCTTGACAGAGAAGCAA+TGG	0.534469	4.3:-19454974	MS.gene28725:CDS
ATGTAGAAAACAAAACTGCT+TGG	0.538313	4.3:-19455197	MS.gene28725:CDS
GCAGGTGTTTGTCCTACTGT+AGG	0.539536	4.3:-19455102	MS.gene28725:CDS
GTTTGTCCTACTGTAGGTGT+TGG	0.546834	4.3:-19455096	MS.gene28725:CDS
GTTTACTGTTCCAAGAACAT+TGG	0.547876	4.3:-19454848	MS.gene28725:CDS
CGTCCCTTGGACACATGAGA+AGG	0.549392	4.3:+19454013	None:intergenic
TGATGAAGATGGTGCGGAAG+AGG	0.557116	4.3:-19454419	MS.gene28725:CDS
CAACAGAACACTAGCTGCAA+AGG	0.565270	4.3:-19454518	MS.gene28725:CDS
TTTCTTGACAGAGAAGCAAT+GGG	0.565816	4.3:-19454973	MS.gene28725:CDS
GGTGGACACTTCAGTGGTGG+TGG	0.566542	4.3:-19455075	MS.gene28725:CDS
TATACCTTCTCATGTGTCCA+AGG	0.569407	4.3:-19454017	MS.gene28725:CDS
TATAAGGTTCCATATCTATG+TGG	0.572757	4.3:+19454331	None:intergenic
AAATTCAGTATGCAGTGCAC+TGG	0.574982	4.3:-19454306	MS.gene28725:CDS
GTTCAATCTGGTGCAACACT+TGG	0.575161	4.3:-19455174	MS.gene28725:CDS
ACATAATTGATGTGAAAGGT+AGG	0.586594	4.3:-19454996	MS.gene28725:CDS
AAATTAATATTATCTTGCAA+AGG	0.587351	4.3:-19454759	MS.gene28725:CDS
AAGATAGACCCTCGAAGTCG+TGG	0.589802	4.3:+19455280	None:intergenic
ATGCAACAAAGCTTGTTCAC+AGG	0.593221	4.3:-19454819	MS.gene28725:CDS
GCACCATCTTCATCAAACAT+AGG	0.602030	4.3:+19454427	None:intergenic
ATCTACACTTCCTAGAAACA+AGG	0.610016	4.3:+19454675	None:intergenic
CAAATTCGAATTCGTAGCGG+TGG	0.611749	4.3:-19455303	MS.gene28725:CDS
GCATCAAGAAGGTGATGAGG+TGG	0.615161	4.3:-19454284	MS.gene28725:CDS
TATAAGCAAGCTAGTATTTG+GGG	0.624423	4.3:-19454127	MS.gene28725:CDS
GTTGGTGGACACTTCAGTGG+TGG	0.625730	4.3:-19455078	MS.gene28725:CDS
GCAGTGCACTGGCATCAAGA+AGG	0.625900	4.3:-19454295	MS.gene28725:CDS
AGCGGTGGCCACGACTTCGA+GGG	0.628178	4.3:-19455288	MS.gene28725:CDS
CAACAAAGCTTGTTCACAGG+TGG	0.634425	4.3:-19454816	MS.gene28725:CDS
GGTGTTGGTGGACACTTCAG+TGG	0.638405	4.3:-19455081	MS.gene28725:CDS
CAGTAGGACAAACACCTGCA+GGG	0.647987	4.3:+19455106	None:intergenic
AAGACCGTATTTACGCAACA+AGG	0.649418	4.3:+19455044	None:intergenic
ATACCTTCTCATGTGTCCAA+GGG	0.652262	4.3:-19454016	MS.gene28725:CDS
GCTCACATAATTGATGTGAA+AGG	0.660187	4.3:-19455000	MS.gene28725:CDS
TGTCCACCAACACCTACAGT+AGG	0.663218	4.3:+19455090	None:intergenic
GTTGCGTAAATACGGTCTTG+CGG	0.685466	4.3:-19455040	MS.gene28725:CDS
CTGGCATCAAGAAGGTGATG+AGG	0.689653	4.3:-19454287	MS.gene28725:CDS
TATGTTTGATGAAGATGGTG+CGG	0.701729	4.3:-19454425	MS.gene28725:CDS
ATGCAAATTCGAATTCGTAG+CGG	0.744504	4.3:-19455306	MS.gene28725:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTAATATTATCTTGCAA+AGG	-	chr4.3:19454553-19454572	MS.gene28725:CDS	15.0%
!!	TAGAAAACTTTATAGTTATA+TGG	-	chr4.3:19455073-19455092	MS.gene28725:CDS	15.0%
!!	AATTATAGAGATCTTGATAT+TGG	-	chr4.3:19455134-19455153	MS.gene28725:CDS	20.0%
!!	GATTTGAATTCAAGTAAAAA+AGG	-	chr4.3:19454579-19454598	MS.gene28725:CDS	20.0%
!	ATTGGTGTGAATAACATTAA+TGG	-	chr4.3:19455152-19455171	MS.gene28725:CDS	25.0%
!	TAATTAATATATGCAGCTCT+TGG	+	chr4.3:19455119-19455138	None:intergenic	25.0%
!!	TGCATACTGAATTTTGTATA+AGG	+	chr4.3:19455000-19455019	None:intergenic	25.0%
!!	TTATTTCAATCCTTGTTTCT+AGG	-	chr4.3:19454627-19454646	MS.gene28725:CDS	25.0%
	AAAATTCCTATTCCTGAAAC+TGG	-	chr4.3:19454846-19454865	MS.gene28725:CDS	30.0%
	AAGAAAAGTTTCCTGAACTT+GGG	-	chr4.3:19454679-19454698	MS.gene28725:CDS	30.0%
	AAGATTGTGTTGAAATGAGT+TGG	-	chr4.3:19454712-19454731	MS.gene28725:CDS	30.0%
	ACATAATTGATGTGAAAGGT+AGG	-	chr4.3:19454316-19454335	MS.gene28725:CDS	30.0%
	ATGTAGAAAACAAAACTGCT+TGG	-	chr4.3:19454115-19454134	MS.gene28725:CDS	30.0%
	ATTCCATTTCCACATAGATA+TGG	-	chr4.3:19454972-19454991	MS.gene28725:CDS	30.0%
	CAAGAACAACTTCAAAAGAT+TGG	-	chr4.3:19455220-19455239	MS.gene28725:CDS	30.0%
	CTAATGGTATTGTTTCCTTA+TGG	-	chr4.3:19454918-19454937	MS.gene28725:CDS	30.0%
	CTATAAGCAAGCTAGTATTT+GGG	-	chr4.3:19455184-19455203	MS.gene28725:CDS	30.0%
	TATAAGCAAGCTAGTATTTG+GGG	-	chr4.3:19455185-19455204	MS.gene28725:CDS	30.0%
	TATAAGGTTCCATATCTATG+TGG	+	chr4.3:19454984-19455003	None:intergenic	30.0%
	TGTAGAAAACAAAACTGCTT+GGG	-	chr4.3:19454116-19454135	MS.gene28725:CDS	30.0%
!	AAACTGGTTTAGAAGGATTA+TGG	-	chr4.3:19454862-19454881	MS.gene28725:CDS	30.0%
!	TCATTTCTGAAGAAGTTTAG+AGG	+	chr4.3:19455269-19455288	None:intergenic	30.0%
!	TGAAATGAGTTGGATTGAAT+CGG	-	chr4.3:19454722-19454741	MS.gene28725:CDS	30.0%
!!	AATCGGTTCTTTACCTTTTT+CGG	-	chr4.3:19454739-19454758	MS.gene28725:CDS	30.0%
!!	AATGGGTGAAGATTTGTTTT+GGG	-	chr4.3:19454356-19454375	MS.gene28725:CDS	30.0%
!!!	AACCAAGAGTTTTGCTTTTT+TGG	+	chr4.3:19454185-19454204	None:intergenic	30.0%
	AATCCTTCTAAACCAGTTTC+AGG	+	chr4.3:19454861-19454880	None:intergenic	35.0%
	AGCCAAAAAAGCAAAACTCT+TGG	-	chr4.3:19454180-19454199	MS.gene28725:CDS	35.0%
	ATCTACACTTCCTAGAAACA+AGG	+	chr4.3:19454640-19454659	None:intergenic	35.0%
	ATGCAAATTCGAATTCGTAG+CGG	-	chr4.3:19454006-19454025	MS.gene28725:CDS	35.0%
	ATGGTATTGTTTCCTTATGG+TGG	-	chr4.3:19454921-19454940	MS.gene28725:CDS	35.0%
	GAAGAAAAGTTTCCTGAACT+TGG	-	chr4.3:19454678-19454697	MS.gene28725:CDS	35.0%
	GAAGTTTAGAGGATCAACTT+TGG	+	chr4.3:19455258-19455277	None:intergenic	35.0%
	GACAAAATCAGATTTCGCTT+TGG	+	chr4.3:19454826-19454845	None:intergenic	35.0%
	GCTATAAGCAAGCTAGTATT+TGG	-	chr4.3:19455183-19455202	MS.gene28725:CDS	35.0%
	GCTCACATAATTGATGTGAA+AGG	-	chr4.3:19454312-19454331	MS.gene28725:CDS	35.0%
	GTAAACACTGTTACAGTTGA+TGG	+	chr4.3:19454450-19454469	None:intergenic	35.0%
	GTGAGATCAATGATGACAAA+AGG	+	chr4.3:19454072-19454091	None:intergenic	35.0%
	GTGAGCATCAATGATGTTAT+CGG	+	chr4.3:19454298-19454317	None:intergenic	35.0%
	GTTCCATATCTATGTGGAAA+TGG	+	chr4.3:19454978-19454997	None:intergenic	35.0%
	GTTTACTGTTCCAAGAACAT+TGG	-	chr4.3:19454464-19454483	MS.gene28725:CDS	35.0%
	TATGTTTGATGAAGATGGTG+CGG	-	chr4.3:19454887-19454906	MS.gene28725:CDS	35.0%
	TCTAAACCAGTTTCAGGAAT+AGG	+	chr4.3:19454855-19454874	None:intergenic	35.0%
	TTATCCATTATGCCACCATA+AGG	+	chr4.3:19454936-19454955	None:intergenic	35.0%
	TTTCTTGACAGAGAAGCAAT+GGG	-	chr4.3:19454339-19454358	MS.gene28725:CDS	35.0%
!	AGGAAACTTTTCTTCCATCA+AGG	+	chr4.3:19454673-19454692	None:intergenic	35.0%
!	ATTCCTGAAACTGGTTTAGA+AGG	-	chr4.3:19454855-19454874	MS.gene28725:CDS	35.0%
!	GCATTTTGTTCCAATGTTCT+TGG	+	chr4.3:19454477-19454496	None:intergenic	35.0%
!	GGAAACTTTTCTTCCATCAA+GGG	+	chr4.3:19454672-19454691	None:intergenic	35.0%
!!	AAAGCTAGTACTGTTGACTT+TGG	+	chr4.3:19454609-19454628	None:intergenic	35.0%
!!	CAATGGGTGAAGATTTGTTT+TGG	-	chr4.3:19454355-19454374	MS.gene28725:CDS	35.0%
!!	CTTGGAGACTTTGAAACAAA+AGG	+	chr4.3:19455101-19455120	None:intergenic	35.0%
!!!	GATTTGTTTTGGGCTATTAG+AGG	-	chr4.3:19454366-19454385	MS.gene28725:CDS	35.0%
!!!	GTTTTGGTGTCATAGTTTCA+TGG	-	chr4.3:19454403-19454422	MS.gene28725:CDS	35.0%
	AAAACTGCTTGGGTTCAATC+TGG	-	chr4.3:19454126-19454145	MS.gene28725:CDS	40.0%
	AAAGTTTCCTGAACTTGGGT+TGG	-	chr4.3:19454683-19454702	MS.gene28725:CDS	40.0%
	AAATTCAGTATGCAGTGCAC+TGG	-	chr4.3:19455006-19455025	MS.gene28725:CDS	40.0%
	AAGACCGTATTTACGCAACA+AGG	+	chr4.3:19454271-19454290	None:intergenic	40.0%
	AGATAACCTCTTACCCTTGA+TGG	-	chr4.3:19454656-19454675	MS.gene28725:CDS	40.0%
	ATACCTTCTCATGTGTCCAA+GGG	-	chr4.3:19455296-19455315	MS.gene28725:CDS	40.0%
	GCACCATCTTCATCAAACAT+AGG	+	chr4.3:19454888-19454907	None:intergenic	40.0%
	GGAACCTTGTTGCGTAAATA+CGG	-	chr4.3:19454264-19454283	MS.gene28725:CDS	40.0%
	GTTTCCTTATGGTGGCATAA+TGG	-	chr4.3:19454929-19454948	MS.gene28725:CDS	40.0%
	GTTTCTTGACAGAGAAGCAA+TGG	-	chr4.3:19454338-19454357	MS.gene28725:CDS	40.0%
	TATACCTTCTCATGTGTCCA+AGG	-	chr4.3:19455295-19455314	MS.gene28725:CDS	40.0%
	TGGAGAAGTTGCACATCAAT+TGG	-	chr4.3:19455048-19455067	MS.gene28725:CDS	40.0%
	TGGCCTATGTTTGATGAAGA+TGG	-	chr4.3:19454882-19454901	MS.gene28725:CDS	40.0%
	TTTCTTCCATCAAGGGTAAG+AGG	+	chr4.3:19454665-19454684	None:intergenic	40.0%
!	ATGCAACAAAGCTTGTTCAC+AGG	-	chr4.3:19454493-19454512	MS.gene28725:CDS	40.0%
!!!	TACCTTTTTCGGTTTCCAGA+AGG	-	chr4.3:19454750-19454769	MS.gene28725:CDS	40.0%
!!!	TTGTTTTGGGCTATTAGAGG+TGG	-	chr4.3:19454369-19454388	MS.gene28725:CDS	40.0%
	AAAACTCTTGGTTTCCCTGC+AGG	-	chr4.3:19454192-19454211	MS.gene28725:CDS	45.0%
	CAAATTCGAATTCGTAGCGG+TGG	-	chr4.3:19454009-19454028	MS.gene28725:CDS	45.0%
	CAACAGAACACTAGCTGCAA+AGG	-	chr4.3:19454794-19454813	MS.gene28725:CDS	45.0%
	CTGTTGAGCAACGTTTCAAG+TGG	+	chr4.3:19454780-19454799	None:intergenic	45.0%
	GTTCAATCTGGTGCAACACT+TGG	-	chr4.3:19454138-19454157	MS.gene28725:CDS	45.0%
	GTTGCGTAAATACGGTCTTG+CGG	-	chr4.3:19454272-19454291	MS.gene28725:CDS	45.0%
	GTTTGTCCTACTGTAGGTGT+TGG	-	chr4.3:19454216-19454235	MS.gene28725:CDS	45.0%
	TCTCTAACCAACCCAAGTTC+AGG	+	chr4.3:19454693-19454712	None:intergenic	45.0%
!	CAACAAAGCTTGTTCACAGG+TGG	-	chr4.3:19454496-19454515	MS.gene28725:CDS	45.0%
!	TTTTGGGCTATTAGAGGTGG+TGG	-	chr4.3:19454372-19454391	MS.gene28725:CDS	45.0%
	AAGATAGACCCTCGAAGTCG+TGG	+	chr4.3:19454035-19454054	None:intergenic	50.0%
	ACAGTAGGACAAACACCTGC+AGG	+	chr4.3:19454210-19454229	None:intergenic	50.0%
	CAGTAGGACAAACACCTGCA+GGG	+	chr4.3:19454209-19454228	None:intergenic	50.0%
	CTGGCATCAAGAAGGTGATG+AGG	-	chr4.3:19455025-19455044	MS.gene28725:CDS	50.0%
	GCAGGTGTTTGTCCTACTGT+AGG	-	chr4.3:19454210-19454229	MS.gene28725:CDS	50.0%
	GCATCAAGAAGGTGATGAGG+TGG	-	chr4.3:19455028-19455047	MS.gene28725:CDS	50.0%
	TGATGAAGATGGTGCGGAAG+AGG	-	chr4.3:19454893-19454912	MS.gene28725:CDS	50.0%
	TGTCCACCAACACCTACAGT+AGG	+	chr4.3:19454225-19454244	None:intergenic	50.0%
!	CGCCTTCTGGAAACCGAAAA+AGG	+	chr4.3:19454755-19454774	None:intergenic	50.0%
!	GTTTCAAGTGGTTCGCCTTC+TGG	+	chr4.3:19454768-19454787	None:intergenic	50.0%
!!	CACTTCAGTGGTGGTGGATA+TGG	-	chr4.3:19454243-19454262	MS.gene28725:CDS	50.0%
!!	TGGTGCGGAAGAGGTACTAA+TGG	-	chr4.3:19454902-19454921	MS.gene28725:CDS	50.0%
!!	TGTCCTACTGTAGGTGTTGG+TGG	-	chr4.3:19454219-19454238	MS.gene28725:CDS	50.0%
	CGTCCCTTGGACACATGAGA+AGG	+	chr4.3:19455302-19455321	None:intergenic	55.0%
!	GCAGTGCACTGGCATCAAGA+AGG	-	chr4.3:19455017-19455036	MS.gene28725:CDS	55.0%
!	GTTGGTGGACACTTCAGTGG+TGG	-	chr4.3:19454234-19454253	MS.gene28725:CDS	55.0%
!	TGGGCTATTAGAGGTGGTGG+AGG	-	chr4.3:19454375-19454394	MS.gene28725:CDS	55.0%
!!	GGTGTTGGTGGACACTTCAG+TGG	-	chr4.3:19454231-19454250	MS.gene28725:CDS	55.0%
!!!	GGTGGTGGAGGAGCAAGTTT+TGG	-	chr4.3:19454387-19454406	MS.gene28725:CDS	55.0%
	GGTGGACACTTCAGTGGTGG+TGG	-	chr4.3:19454237-19454256	MS.gene28725:CDS	60.0%
	AGCGGTGGCCACGACTTCGA+GGG	-	chr4.3:19454024-19454043	MS.gene28725:CDS	65.0%
	TAGCGGTGGCCACGACTTCG+AGG	-	chr4.3:19454023-19454042	MS.gene28725:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	19454006	19455328	19454006	ID=MS.gene28725
chr4.3	mRNA	19454006	19455328	19454006	ID=MS.gene28725.t1;Parent=MS.gene28725
chr4.3	exon	19454006	19455328	19454006	ID=MS.gene28725.t1.exon1;Parent=MS.gene28725.t1
chr4.3	CDS	19454006	19455328	19454006	ID=cds.MS.gene28725.t1;Parent=MS.gene28725.t1

Gene Sequence

>MS.gene28725

ATGCAAATTCGAATTCGTAGCGGTGGCCACGACTTCGAGGGTCTATCTTTTGTTTCACATGTTCCTTTTGTCATCATTGATCTCACAAACTTTAGAGAAATTGATGTAGATGTAGAAAACAAAACTGCTTGGGTTCAATCTGGTGCAACACTTGGTGAACTTTACTACAAAATTAGCCAAAAAAGCAAAACTCTTGGTTTCCCTGCAGGTGTTTGTCCTACTGTAGGTGTTGGTGGACACTTCAGTGGTGGTGGATATGGAACCTTGTTGCGTAAATACGGTCTTGCGGCCGATAACATCATTGATGCTCACATAATTGATGTGAAAGGTAGGTTTCTTGACAGAGAAGCAATGGGTGAAGATTTGTTTTGGGCTATTAGAGGTGGTGGAGGAGCAAGTTTTGGTGTCATAGTTTCATGGAAGATCAAACTTGTTCAAGTTCCATCAACTGTAACAGTGTTTACTGTTCCAAGAACATTGGAACAAAATGCAACAAAGCTTGTTCACAGGTGGCAGTATGTAGCACATAAACTTGATGAAAACTTAGAAATTAATATTATCTTGCAAAGGCTAGATTTGAATTCAAGTAAAAAAGGTGAGCCAAAGTCAACAGTACTAGCTTTATTTCAATCCTTGTTTCTAGGAAGTGTAGATAACCTCTTACCCTTGATGGAAGAAAAGTTTCCTGAACTTGGGTTGGTTAGAGAAGATTGTGTTGAAATGAGTTGGATTGAATCGGTTCTTTACCTTTTTCGGTTTCCAGAAGGCGAACCACTTGAAACGTTGCTCAACAGAACACTAGCTGCAAAGGATAATTCCAAAGCGAAATCTGATTTTGTCAAAATTCCTATTCCTGAAACTGGTTTAGAAGGATTATGGCCTATGTTTGATGAAGATGGTGCGGAAGAGGTACTAATGGTATTGTTTCCTTATGGTGGCATAATGGATAAGATTTCAGAATCTGAAATTCCATTTCCACATAGATATGGAACCTTATACAAAATTCAGTATGCAGTGCACTGGCATCAAGAAGGTGATGAGGTGGAGAAGTTGCACATCAATTGGATTAGAAAACTTTATAGTTATATGGAACCTTTTGTTTCAAAGTCTCCAAGAGCTGCATATATTAATTATAGAGATCTTGATATTGGTGTGAATAACATTAATGGATACACAAGCTATAAGCAAGCTAGTATTTGGGGTGTTAAATACTTCAAGAACAACTTCAAAAGATTGGCAAAAGTGAAGACCAAAGTTGATCCTCTAAACTTCTTCAGAAATGAACAGAGTATACCTTCTCATGTGTCCAAGGGACGCAAATAG

Protein sequence

>MS.gene28725.t1

MQIRIRSGGHDFEGLSFVSHVPFVIIDLTNFREIDVDVENKTAWVQSGATLGELYYKISQKSKTLGFPAGVCPTVGVGGHFSGGGYGTLLRKYGLAADNIIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIKLVQVPSTVTVFTVPRTLEQNATKLVHRWQYVAHKLDENLEINIILQRLDLNSSKKGEPKSTVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLFRFPEGEPLETLLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPMFDEDGAEEVLMVLFPYGGIMDKISESEIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINYRDLDIGVNNINGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIPSHVSKGRK