AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28727.t1 MTR_4g094478 95.741 540 23 0 1 540 1 540 0.0 1042
MS.gene28727.t1 MTR_4g094480 85.019 534 80 0 1 534 1 534 0.0 921
MS.gene28727.t1 MTR_4g094488 80.597 536 102 1 1 534 1 536 0.0 885
MS.gene28727.t1 MTR_4g094495 77.551 539 116 2 1 534 1 539 0.0 849
MS.gene28727.t1 MTR_4g094492 76.654 544 122 2 1 541 1 542 0.0 845
MS.gene28727.t1 MTR_4g094418 71.330 436 120 2 104 535 1 435 0.0 659
MS.gene28727.t1 MTR_2g031560 51.816 523 248 2 13 535 5 523 0.0 566
MS.gene28727.t1 MTR_2g031390 52.250 511 237 5 30 538 27 532 0.0 559
MS.gene28727.t1 MTR_2g031590 53.073 537 244 5 2 535 1 532 0.0 550
MS.gene28727.t1 MTR_3g009880 53.232 526 236 6 11 535 4 520 0.0 548
MS.gene28727.t1 MTR_4g094498 56.088 501 213 3 35 535 41 534 0.0 546
MS.gene28727.t1 MTR_3g009760 52.000 525 242 5 13 535 6 522 0.0 544
MS.gene28727.t1 MTR_3g009820 52.000 525 242 5 13 535 6 522 0.0 542
MS.gene28727.t1 MTR_2g031420 53.731 536 233 8 6 535 1 527 0.0 536
MS.gene28727.t1 MTR_4g094520 52.328 537 241 8 1 535 9 532 0.0 530
MS.gene28727.t1 MTR_4g094518 53.543 508 229 5 30 535 28 530 0.0 528
MS.gene28727.t1 MTR_2g031460 53.113 514 231 6 30 543 30 533 0.0 525
MS.gene28727.t1 MTR_2g031490 50.372 538 257 7 3 535 6 538 1.68e-176 509
MS.gene28727.t1 MTR_4g088355 51.866 509 233 5 31 534 31 532 2.83e-172 498
MS.gene28727.t1 MTR_6g017205 49.905 525 253 5 11 534 10 525 9.29e-172 497
MS.gene28727.t1 MTR_4g091200 49.811 530 253 5 6 534 8 525 2.69e-171 495
MS.gene28727.t1 MTR_4g091190 48.405 533 265 6 6 537 7 530 1.88e-170 493
MS.gene28727.t1 MTR_4g091150 51.663 511 234 6 31 535 37 540 2.04e-169 491
MS.gene28727.t1 MTR_4g057990 49.907 535 253 7 1 534 1 521 3.66e-169 489
MS.gene28727.t1 MTR_4g094515 50.847 531 254 4 8 535 624 1150 4.95e-169 509
MS.gene28727.t1 MTR_4g094515 50.791 506 241 5 32 534 39 539 2.72e-163 494
MS.gene28727.t1 MTR_2g031510 50.593 506 242 5 33 535 40 540 6.70e-169 489
MS.gene28727.t1 MTR_2g023590 49.359 468 230 3 68 534 4 465 1.58e-148 435
MS.gene28727.t1 MTR_4g091170 47.300 463 221 5 73 534 101 541 1.14e-146 434
MS.gene28727.t1 MTR_1g076830 40.458 524 295 8 32 545 31 547 3.37e-127 383
MS.gene28727.t1 MTR_3g009850 57.965 226 93 2 310 535 5 228 3.65e-87 269
MS.gene28727.t1 MTR_2g031600 58.130 246 101 1 1 246 1 244 4.86e-86 272
MS.gene28727.t1 MTR_2g031600 52.761 163 73 3 374 535 241 400 3.50e-50 178
MS.gene28727.t1 MTR_2g084370 46.847 111 37 2 128 238 1 89 1.30e-28 109
MS.gene28727.t1 MTR_2g084405 53.165 79 37 0 128 206 1 79 1.71e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28727.t1 AT5G44440 50.471 531 247 7 8 533 8 527 1.70e-179 516
MS.gene28727.t1 AT4G20820 49.348 537 254 5 2 533 1 524 1.89e-179 516
MS.gene28727.t1 AT5G44440 50.471 531 247 7 8 533 78 597 3.06e-178 516
MS.gene28727.t1 AT5G44400 48.704 540 263 9 3 536 6 537 1.90e-177 511
MS.gene28727.t1 AT2G34790 47.925 530 261 8 9 534 11 529 1.73e-175 506
MS.gene28727.t1 AT5G44390 47.698 543 266 11 3 535 6 540 2.37e-172 498
MS.gene28727.t1 AT1G30760 48.924 511 248 6 28 534 48 549 8.08e-171 495
MS.gene28727.t1 AT1G30760 48.924 511 248 6 28 534 30 531 9.09e-171 494
MS.gene28727.t1 AT5G44360 49.174 545 254 11 1 536 1 531 2.47e-169 490
MS.gene28727.t1 AT5G44360 49.174 545 254 11 1 536 17 547 3.66e-169 490
MS.gene28727.t1 AT5G44410 49.440 536 249 9 4 533 8 527 5.03e-169 489
MS.gene28727.t1 AT5G44380 48.799 541 262 8 3 535 6 539 5.48e-166 482
MS.gene28727.t1 AT5G44380 48.799 541 262 8 3 535 46 579 1.20e-165 483
MS.gene28727.t1 AT1G26420 47.070 529 267 9 8 533 5 523 3.70e-161 469
MS.gene28727.t1 AT1G30700 46.904 533 273 6 6 535 1 526 1.95e-160 468
MS.gene28727.t1 AT1G30730 46.958 526 270 5 10 533 5 523 4.12e-160 466
MS.gene28727.t1 AT1G26380 47.388 536 267 10 13 543 10 535 6.19e-160 466
MS.gene28727.t1 AT1G26410 48.538 513 252 8 26 535 46 549 8.79e-160 467
MS.gene28727.t1 AT1G30710 45.421 535 270 9 9 535 8 528 1.27e-158 463
MS.gene28727.t1 AT1G26400 46.679 527 269 7 8 532 5 521 3.32e-158 462
MS.gene28727.t1 AT4G20840 46.758 509 260 6 31 536 36 536 6.06e-158 461
MS.gene28727.t1 AT1G11770 45.810 537 278 7 4 535 1 529 6.12e-158 461
MS.gene28727.t1 AT4G20860 48.767 527 241 12 22 535 19 529 2.79e-157 459
MS.gene28727.t1 AT1G26390 48.134 509 247 5 27 535 36 527 5.97e-156 456
MS.gene28727.t1 AT1G34575 45.966 533 264 10 6 533 9 522 4.48e-153 449
MS.gene28727.t1 AT4G20800 45.508 512 271 5 23 533 19 523 2.51e-151 444
MS.gene28727.t1 AT4G20830 47.244 508 255 7 32 535 38 536 1.07e-149 441
MS.gene28727.t1 AT4G20830 47.244 508 255 7 32 535 38 536 2.91e-149 441
MS.gene28727.t1 AT1G30740 45.455 539 273 10 10 539 9 535 1.51e-146 432
MS.gene28727.t1 AT1G30740 45.269 539 274 10 10 539 6 532 1.60e-146 432
MS.gene28727.t1 AT1G30720 47.117 503 257 5 33 533 29 524 2.72e-146 431
MS.gene28727.t1 AT1G01980 44.981 538 279 9 6 535 6 534 3.17e-145 429
MS.gene28727.t1 AT2G34810 43.922 510 278 5 31 535 33 539 5.19e-138 410

Find 109 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCGGTTCTTTACCTTTA+TGG 0.132034 4.3:-19471647 MS.gene28727:CDS
AAAATTCCCATTCCTGAAAT+TGG 0.132285 4.3:-19471539 MS.gene28727:CDS
AAATTATGGTTGTTTGAATA+TGG 0.144161 4.3:+19472413 None:intergenic
GTTATAAGCAAGCTAGTATT+TGG 0.173497 4.3:-19471202 MS.gene28727:CDS
ATTCCTTCTAATCCAATTTC+AGG 0.177038 4.3:+19471527 None:intergenic
AATCGGTTCTTTACCTTTAT+GGG 0.198752 4.3:-19471646 MS.gene28727:CDS
CCTACCCTTCACATCAATTA+TGG 0.210755 4.3:+19472063 None:intergenic
CACCCGGAAAACCAAGATTT+TGG 0.227676 4.3:+19472188 None:intergenic
TAGAAAACTTTATAGTTATA+TGG 0.249330 4.3:-19471312 MS.gene28727:CDS
ATGGATTTGTGAACAAATTA+TGG 0.257514 4.3:+19472399 None:intergenic
AATTATAGAGATCTTGATAT+TGG 0.259521 4.3:-19471251 MS.gene28727:CDS
CAATGGGTGAAGATTTGTTT+TGG 0.263104 4.3:-19472024 MS.gene28727:CDS
AATGAACTGAGTAATAAATT+TGG 0.287296 4.3:+19471381 None:intergenic
GGTGGTGGAGGAGCAAGTTT+TGG 0.291186 4.3:-19471992 MS.gene28727:CDS
GTTTCAAGTGGTTCGTCTTT+CGG 0.292602 4.3:+19471620 None:intergenic
AAGGGTAAATTCAAGTATAA+AGG 0.303183 4.3:-19471807 MS.gene28727:CDS
GTGAGATCAAGGATGATAAA+TGG 0.304625 4.3:+19472310 None:intergenic
AAATATCACCATAGCATCTT+TGG 0.304978 4.3:+19471478 None:intergenic
ATATTTCAAGCCTTGTTTCT+TGG 0.305951 4.3:-19471758 MS.gene28727:CDS
ATTCCTGAAATTGGATTAGA+AGG 0.307063 4.3:-19471530 MS.gene28727:CDS
CAGAAGATGTATATGAAAAT+AGG 0.308588 4.3:+19472638 None:intergenic
ATTATAGAGATCTTGATATT+GGG 0.316617 4.3:-19471250 MS.gene28727:CDS
ATTCCATTTCCACATAGATA+TGG 0.327139 4.3:-19471413 MS.gene28727:CDS
GGAACCTTGGTTCGTAAATT+CGG 0.334327 4.3:-19472115 MS.gene28727:CDS
AATTATGGTTGTTTGAATAT+GGG 0.337348 4.3:+19472414 None:intergenic
TTAAAGACTGTCACAATTGA+TGG 0.347075 4.3:+19471932 None:intergenic
AGGGTAAATTCAAGTATAAA+GGG 0.347661 4.3:-19471806 MS.gene28727:CDS
GGATTCGAATCTGCAGGTTA+TGG 0.348552 4.3:+19472380 None:intergenic
TTATAAGCAAGCTAGTATTT+GGG 0.359669 4.3:-19471201 MS.gene28727:CDS
TATTGATGAGTAAGAGGAAT+TGG 0.360998 4.3:+19472519 None:intergenic
CTCTGAAGTTTGTGAGATCA+AGG 0.366703 4.3:+19472299 None:intergenic
GTGATAATGACAAGAGGTTT+AGG 0.372360 4.3:+19472451 None:intergenic
CTTGGTGACTTTGAAACAAA+AGG 0.372572 4.3:+19471287 None:intergenic
CGTCTTTCGGAAACCCATAA+AGG 0.375701 4.3:+19471633 None:intergenic
AAATTTATTACTCAGTTCAT+TGG 0.379935 4.3:-19471379 MS.gene28727:CDS
TTTCCATATCTATGTGGAAA+TGG 0.380986 4.3:+19471410 None:intergenic
TTTGAGTATTGATGAGTAAG+AGG 0.381041 4.3:+19472513 None:intergenic
TGAATATGGGAAATCTGTTT+AGG 0.386909 4.3:+19472427 None:intergenic
CAAGAACAACTTCAAAAGAT+TGG 0.391940 4.3:-19471165 MS.gene28727:CDS
ATAGTAAAATATAAGTTTAG+AGG 0.392038 4.3:+19472676 None:intergenic
CACATCAGTGGCGGCGGCTA+TGG 0.400837 4.3:-19472136 MS.gene28727:CDS
AAATTGGATTAGAAGGAATA+TGG 0.403457 4.3:-19471523 MS.gene28727:CDS
CAAGAGAAGTTTCCTGAGCT+TGG 0.412203 4.3:-19471707 MS.gene28727:CDS
TCTAATCCAATTTCAGGAAT+GGG 0.420910 4.3:+19471533 None:intergenic
TACCTTCTCTCATGTGTTCA+AGG 0.422099 4.3:-19471088 MS.gene28727:CDS
TTCTAATCCAATTTCAGGAA+TGG 0.423335 4.3:+19471532 None:intergenic
AAGATTGTGTTGAGATGAGT+TGG 0.428995 4.3:-19471673 MS.gene28727:CDS
TCATTTCTGAAGAAGTTAAG+AGG 0.433059 4.3:+19471119 None:intergenic
CTACCCTTCACATCAATTAT+GGG 0.433986 4.3:+19472064 None:intergenic
GAAGTTTCCTGAGCTTGGTT+TGG 0.437875 4.3:-19471702 MS.gene28727:CDS
TTTCAAGCCTTGTTTCTTGG+AGG 0.440275 4.3:-19471755 MS.gene28727:CDS
CAAAGTCATGGCCACCGCTT+CGG 0.450183 4.3:+19472359 None:intergenic
AAGCGGTGGCCATGACTTTG+AGG 0.450822 4.3:-19472356 MS.gene28727:CDS
TGAGATCAAGGATGATAAAT+GGG 0.474438 4.3:+19472311 None:intergenic
GGTGGTGAATGTCCTACTAT+AGG 0.479825 4.3:-19472169 MS.gene28727:CDS
AGAACAGCTTGGGTTCAATC+TGG 0.484147 4.3:-19472253 MS.gene28727:CDS
TGGGCTATTAGAGGTGGTGG+AGG 0.490480 4.3:-19472004 MS.gene28727:CDS
AATTACTGTTATCTTACAAA+GGG 0.491690 4.3:-19471825 MS.gene28727:CDS
TGAGTAAGAGGAATTGGCTT+TGG 0.492341 4.3:+19472525 None:intergenic
TGTAGAAAGCAGAACAGCTT+GGG 0.494574 4.3:-19472263 MS.gene28727:CDS
CTATCCATTATGCCACCATA+AGG 0.494662 4.3:+19471452 None:intergenic
TGAGATGAGTTGGGTTGAAT+CGG 0.513533 4.3:-19471663 MS.gene28727:CDS
GTTCAATCTGGTGCAACAAT+TGG 0.515144 4.3:-19472241 MS.gene28727:CDS
TAATTGATATATGCAGCTCT+TGG 0.521563 4.3:+19471269 None:intergenic
AGATTGTGTTGAGATGAGTT+GGG 0.523370 4.3:-19471672 MS.gene28727:CDS
TCTCTAACCAAACCAAGCTC+AGG 0.524526 4.3:+19471695 None:intergenic
GACAAAGAAATCAGCAGCTA+AGG 0.525403 4.3:-19471065 None:intergenic
CTGTTGAGCAACGTTTCAAG+TGG 0.530743 4.3:+19471608 None:intergenic
CGCTTCGGATTCGAATCTGC+AGG 0.531779 4.3:+19472374 None:intergenic
AGCGGTGGCCATGACTTTGA+GGG 0.532159 4.3:-19472355 MS.gene28727:CDS
GTTTCTTGACAGAGAAGCAA+TGG 0.534469 4.3:-19472041 MS.gene28727:CDS
AAGAGGTTTAGGTGTTGTGT+TGG 0.535453 4.3:+19472462 None:intergenic
AAATGGGACTTCAGAAACAT+AGG 0.536814 4.3:+19472327 None:intergenic
TATAAATTTCCATATCTATG+TGG 0.540221 4.3:+19471404 None:intergenic
TGTCCACCAACACCTATAGT+AGG 0.545211 4.3:+19472157 None:intergenic
TGGCGGCGGCTATGGAACCT+TGG 0.554368 4.3:-19472128 MS.gene28727:CDS
GGTGGACACATCAGTGGCGG+CGG 0.558776 4.3:-19472142 MS.gene28727:CDS
TGGCCTATGTTTCATGATGA+TGG 0.560963 4.3:-19471503 MS.gene28727:CDS
GCACCATCATCATGAAACAT+AGG 0.563262 4.3:+19471500 None:intergenic
CAACAGAACACTAGCTGCAA+AGG 0.565270 4.3:-19471591 MS.gene28727:CDS
TTTCTTGACAGAGAAGCAAT+GGG 0.565816 4.3:-19472040 MS.gene28727:CDS
AAATTACTGTTATCTTACAA+AGG 0.566762 4.3:-19471826 MS.gene28727:CDS
TCCCTTGAACACATGAGAGA+AGG 0.572099 4.3:+19471086 None:intergenic
ATGTAGAAAGCAGAACAGCT+TGG 0.572213 4.3:-19472264 MS.gene28727:CDS
TTATAGAGATCTTGATATTG+GGG 0.576997 4.3:-19471249 MS.gene28727:CDS
GCATCAAGAAAGTGATGAGG+TGG 0.587589 4.3:-19471357 MS.gene28727:CDS
CAACAAAGCTTATTCACAAG+TGG 0.589114 4.3:-19471883 MS.gene28727:CDS
TGCTCCCATAATTGATGTGA+AGG 0.590190 4.3:-19472068 MS.gene28727:CDS
TGTCCTACTATAGGTGTTGG+TGG 0.598722 4.3:-19472160 MS.gene28727:CDS
GAATGTCCTACTATAGGTGT+TGG 0.600015 4.3:-19472163 MS.gene28727:CDS
CCATAATTGATGTGAAGGGT+AGG 0.600117 4.3:-19472063 MS.gene28727:CDS
GTGGCCATGACTTTGAGGGT+CGG 0.601490 4.3:-19472351 MS.gene28727:CDS
ACCTTCTCTCATGTGTTCAA+GGG 0.603782 4.3:-19471087 MS.gene28727:CDS
ATTGGGGTGAATAATAACAA+TGG 0.606283 4.3:-19471233 MS.gene28727:CDS
TATAAGCAAGCTAGTATTTG+GGG 0.615112 4.3:-19471200 MS.gene28727:CDS
TGATGGTGCCAAAGATGCTA+TGG 0.619348 4.3:-19471486 MS.gene28727:CDS
CAGATTCGAATCCGAAGCGG+TGG 0.621977 4.3:-19472370 MS.gene28727:CDS
ATCTACACCTCCAAGAAACA+AGG 0.636642 4.3:+19471748 None:intergenic
GGGTAAATTCAAGTATAAAG+GGG 0.641672 4.3:-19471805 MS.gene28727:CDS
AGGACCGACCCTCAAAGTCA+TGG 0.642967 4.3:+19472347 None:intergenic
GGTGTTGGTGGACACATCAG+TGG 0.646578 4.3:-19472148 MS.gene28727:CDS
GTTGGTGGACACATCAGTGG+CGG 0.651631 4.3:-19472145 MS.gene28727:CDS
AATAACTATAGAGACACTGA+AGG 0.658653 4.3:+19472584 None:intergenic
TTAGGTGTGATAATGACAAG+AGG 0.664428 4.3:+19472445 None:intergenic
AAGACCGAATTTACGAACCA+AGG 0.678437 4.3:+19472111 None:intergenic
ATAGTAGGACATTCACCACC+CGG 0.688594 4.3:+19472172 None:intergenic
GCTCCCATAATTGATGTGAA+GGG 0.694549 4.3:-19472067 MS.gene28727:CDS
TTGGCATCAAGAAAGTGATG+AGG 0.701714 4.3:-19471360 MS.gene28727:CDS
CTGCAGATTCGAATCCGAAG+CGG 0.713192 4.3:-19472373 MS.gene28727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAAAATATAAGTTTAG+AGG + chr4.3:19471076-19471095 None:intergenic 15.0%
!! TAGAAAACTTTATAGTTATA+TGG - chr4.3:19472437-19472456 MS.gene28727:CDS 15.0%
!! AAATTACTGTTATCTTACAA+AGG - chr4.3:19471923-19471942 MS.gene28727:CDS 20.0%
!! AAATTATGGTTGTTTGAATA+TGG + chr4.3:19471339-19471358 None:intergenic 20.0%
!! AAATTTATTACTCAGTTCAT+TGG - chr4.3:19472370-19472389 MS.gene28727:CDS 20.0%
!! AATGAACTGAGTAATAAATT+TGG + chr4.3:19472371-19472390 None:intergenic 20.0%
!! AATTACTGTTATCTTACAAA+GGG - chr4.3:19471924-19471943 MS.gene28727:CDS 20.0%
!! AATTATAGAGATCTTGATAT+TGG - chr4.3:19472498-19472517 MS.gene28727:CDS 20.0%
!! AATTATGGTTGTTTGAATAT+GGG + chr4.3:19471338-19471357 None:intergenic 20.0%
!! ATTATAGAGATCTTGATATT+GGG - chr4.3:19472499-19472518 MS.gene28727:CDS 20.0%
!! TATAAATTTCCATATCTATG+TGG + chr4.3:19472348-19472367 None:intergenic 20.0%
!! TGGAAAAAATGAAAATGAAT+TGG - chr4.3:19472412-19472431 MS.gene28727:CDS 20.0%
! AAGGGTAAATTCAAGTATAA+AGG - chr4.3:19471942-19471961 MS.gene28727:CDS 25.0%
! AGGGTAAATTCAAGTATAAA+GGG - chr4.3:19471943-19471962 MS.gene28727:CDS 25.0%
! ATGGATTTGTGAACAAATTA+TGG + chr4.3:19471353-19471372 None:intergenic 25.0%
! CAGAAGATGTATATGAAAAT+AGG + chr4.3:19471114-19471133 None:intergenic 25.0%
! CTTTAATGTTCCAAAAACAT+TGG - chr4.3:19471834-19471853 MS.gene28727:CDS 25.0%
! TTATAAGCAAGCTAGTATTT+GGG - chr4.3:19472548-19472567 MS.gene28727:CDS 25.0%
! TTATAGAGATCTTGATATTG+GGG - chr4.3:19472500-19472519 MS.gene28727:CDS 25.0%
!! AAATTGGATTAGAAGGAATA+TGG - chr4.3:19472226-19472245 MS.gene28727:CDS 25.0%
!!! AACCAAGATTTTGGTTTTTT+CGG + chr4.3:19471555-19471574 None:intergenic 25.0%
!!! ATGGTGATATTTTTTCCTTA+TGG - chr4.3:19472282-19472301 MS.gene28727:CDS 25.0%
AAAATTCCCATTCCTGAAAT+TGG - chr4.3:19472210-19472229 MS.gene28727:CDS 30.0%
AAATATCACCATAGCATCTT+TGG + chr4.3:19472274-19472293 None:intergenic 30.0%
AATAACTATAGAGACACTGA+AGG + chr4.3:19471168-19471187 None:intergenic 30.0%
ATTCCATTTCCACATAGATA+TGG - chr4.3:19472336-19472355 MS.gene28727:CDS 30.0%
ATTCCTTCTAATCCAATTTC+AGG + chr4.3:19472225-19472244 None:intergenic 30.0%
ATTGGGGTGAATAATAACAA+TGG - chr4.3:19472516-19472535 MS.gene28727:CDS 30.0%
CAAGAACAACTTCAAAAGAT+TGG - chr4.3:19472584-19472603 MS.gene28727:CDS 30.0%
GGGTAAATTCAAGTATAAAG+GGG - chr4.3:19471944-19471963 MS.gene28727:CDS 30.0%
GTTATAAGCAAGCTAGTATT+TGG - chr4.3:19472547-19472566 MS.gene28727:CDS 30.0%
TAATTGATATATGCAGCTCT+TGG + chr4.3:19472483-19472502 None:intergenic 30.0%
TACCGAAAAAACCAAAATCT+TGG - chr4.3:19471550-19471569 MS.gene28727:CDS 30.0%
TATAAGCAAGCTAGTATTTG+GGG - chr4.3:19472549-19472568 MS.gene28727:CDS 30.0%
TATTGATGAGTAAGAGGAAT+TGG + chr4.3:19471233-19471252 None:intergenic 30.0%
TCTAATCCAATTTCAGGAAT+GGG + chr4.3:19472219-19472238 None:intergenic 30.0%
TGAATATGGGAAATCTGTTT+AGG + chr4.3:19471325-19471344 None:intergenic 30.0%
TGAGATCAAGGATGATAAAT+GGG + chr4.3:19471441-19471460 None:intergenic 30.0%
TTAAAGACTGTCACAATTGA+TGG + chr4.3:19471820-19471839 None:intergenic 30.0%
TTCTAATCCAATTTCAGGAA+TGG + chr4.3:19472220-19472239 None:intergenic 30.0%
TTTCCATATCTATGTGGAAA+TGG + chr4.3:19472342-19472361 None:intergenic 30.0%
! AATCGGTTCTTTACCTTTAT+GGG - chr4.3:19472103-19472122 MS.gene28727:CDS 30.0%
! ATTCCTGAAATTGGATTAGA+AGG - chr4.3:19472219-19472238 MS.gene28727:CDS 30.0%
! GCATTTTGTTCCAATGTTTT+TGG + chr4.3:19471847-19471866 None:intergenic 30.0%
! TCATTTCTGAAGAAGTTAAG+AGG + chr4.3:19472633-19472652 None:intergenic 30.0%
!! AAACCAAAATCTTGGTTTTC+CGG - chr4.3:19471558-19471577 MS.gene28727:CDS 30.0%
!! AATGGGTGAAGATTTGTTTT+GGG - chr4.3:19471726-19471745 MS.gene28727:CDS 30.0%
!! ATATTTCAAGCCTTGTTTCT+TGG - chr4.3:19471991-19472010 MS.gene28727:CDS 30.0%
!! TTTGAGTATTGATGAGTAAG+AGG + chr4.3:19471239-19471258 None:intergenic 30.0%
!!! GTGATATTTTTTCCTTATGG+TGG - chr4.3:19472285-19472304 MS.gene28727:CDS 30.0%
!!! GTTTTGGAGTCATAATTTCA+TGG - chr4.3:19471773-19471792 MS.gene28727:CDS 30.0%
AAATGGGACTTCAGAAACAT+AGG + chr4.3:19471425-19471444 None:intergenic 35.0%
AAGATTGTGTTGAGATGAGT+TGG - chr4.3:19472076-19472095 MS.gene28727:CDS 35.0%
AGATTGTGTTGAGATGAGTT+GGG - chr4.3:19472077-19472096 MS.gene28727:CDS 35.0%
CAACAAAGCTTATTCACAAG+TGG - chr4.3:19471866-19471885 MS.gene28727:CDS 35.0%
CTACCCTTCACATCAATTAT+GGG + chr4.3:19471688-19471707 None:intergenic 35.0%
GTGAGATCAAGGATGATAAA+TGG + chr4.3:19471442-19471461 None:intergenic 35.0%
TTTCTTGACAGAGAAGCAAT+GGG - chr4.3:19471709-19471728 MS.gene28727:CDS 35.0%
! GAATCGGTTCTTTACCTTTA+TGG - chr4.3:19472102-19472121 MS.gene28727:CDS 35.0%
! GTGATAATGACAAGAGGTTT+AGG + chr4.3:19471301-19471320 None:intergenic 35.0%
! TTAGGTGTGATAATGACAAG+AGG + chr4.3:19471307-19471326 None:intergenic 35.0%
! TTTTCCTTATGGTGGCATAA+TGG - chr4.3:19472293-19472312 MS.gene28727:CDS 35.0%
!! AACCAAAATCTTGGTTTTCC+GGG - chr4.3:19471559-19471578 MS.gene28727:CDS 35.0%
!! CAATGGGTGAAGATTTGTTT+TGG - chr4.3:19471725-19471744 MS.gene28727:CDS 35.0%
!! CTTGGTGACTTTGAAACAAA+AGG + chr4.3:19472465-19472484 None:intergenic 35.0%
!!! GATTTGTTTTGGGCTATTAG+AGG - chr4.3:19471736-19471755 MS.gene28727:CDS 35.0%
AAGACCGAATTTACGAACCA+AGG + chr4.3:19471641-19471660 None:intergenic 40.0%
ACCTTCTCTCATGTGTTCAA+GGG - chr4.3:19472662-19472681 MS.gene28727:CDS 40.0%
ATCTACACCTCCAAGAAACA+AGG + chr4.3:19472004-19472023 None:intergenic 40.0%
ATGTAGAAAGCAGAACAGCT+TGG - chr4.3:19471485-19471504 MS.gene28727:CDS 40.0%
CCATAATTGATGTGAAGGGT+AGG - chr4.3:19471686-19471705 MS.gene28727:CDS 40.0%
CCTACCCTTCACATCAATTA+TGG + chr4.3:19471689-19471708 None:intergenic 40.0%
CTATCCATTATGCCACCATA+AGG + chr4.3:19472300-19472319 None:intergenic 40.0%
GAATGTCCTACTATAGGTGT+TGG - chr4.3:19471586-19471605 MS.gene28727:CDS 40.0%
GCACCATCATCATGAAACAT+AGG + chr4.3:19472252-19472271 None:intergenic 40.0%
GCTCCCATAATTGATGTGAA+GGG - chr4.3:19471682-19471701 MS.gene28727:CDS 40.0%
GTTCAATCTGGTGCAACAAT+TGG - chr4.3:19471508-19471527 MS.gene28727:CDS 40.0%
GTTTCTTGACAGAGAAGCAA+TGG - chr4.3:19471708-19471727 MS.gene28727:CDS 40.0%
TACCTTCTCTCATGTGTTCA+AGG - chr4.3:19472661-19472680 MS.gene28727:CDS 40.0%
TGCTCCCATAATTGATGTGA+AGG - chr4.3:19471681-19471700 MS.gene28727:CDS 40.0%
TGGCCTATGTTTCATGATGA+TGG - chr4.3:19472246-19472265 MS.gene28727:CDS 40.0%
TGTAGAAAGCAGAACAGCTT+GGG - chr4.3:19471486-19471505 MS.gene28727:CDS 40.0%
TTGGCATCAAGAAAGTGATG+AGG - chr4.3:19472389-19472408 MS.gene28727:CDS 40.0%
! AAGAGGTTTAGGTGTTGTGT+TGG + chr4.3:19471290-19471309 None:intergenic 40.0%
! GGAACCTTGGTTCGTAAATT+CGG - chr4.3:19471634-19471653 MS.gene28727:CDS 40.0%
! GTTTCAAGTGGTTCGTCTTT+CGG + chr4.3:19472132-19472151 None:intergenic 40.0%
! TGAGTAAGAGGAATTGGCTT+TGG + chr4.3:19471227-19471246 None:intergenic 40.0%
!! CTCTGAAGTTTGTGAGATCA+AGG + chr4.3:19471453-19471472 None:intergenic 40.0%
!! TGAGATGAGTTGGGTTGAAT+CGG - chr4.3:19472086-19472105 MS.gene28727:CDS 40.0%
!! TTTCAAGCCTTGTTTCTTGG+AGG - chr4.3:19471994-19472013 MS.gene28727:CDS 40.0%
!!! TTGTTTTGGGCTATTAGAGG+TGG - chr4.3:19471739-19471758 MS.gene28727:CDS 40.0%
AGAACAGCTTGGGTTCAATC+TGG - chr4.3:19471496-19471515 MS.gene28727:CDS 45.0%
ATAGTAGGACATTCACCACC+CGG + chr4.3:19471580-19471599 None:intergenic 45.0%
CAACAGAACACTAGCTGCAA+AGG - chr4.3:19472158-19472177 MS.gene28727:CDS 45.0%
CAAGAGAAGTTTCCTGAGCT+TGG - chr4.3:19472042-19472061 MS.gene28727:CDS 45.0%
CGTCTTTCGGAAACCCATAA+AGG + chr4.3:19472119-19472138 None:intergenic 45.0%
CTGTTGAGCAACGTTTCAAG+TGG + chr4.3:19472144-19472163 None:intergenic 45.0%
GAAGTTTCCTGAGCTTGGTT+TGG - chr4.3:19472047-19472066 MS.gene28727:CDS 45.0%
GCATCAAGAAAGTGATGAGG+TGG - chr4.3:19472392-19472411 MS.gene28727:CDS 45.0%
GGATTCGAATCTGCAGGTTA+TGG + chr4.3:19471372-19471391 None:intergenic 45.0%
GGTGGTGAATGTCCTACTAT+AGG - chr4.3:19471580-19471599 MS.gene28727:CDS 45.0%
TCCCTTGAACACATGAGAGA+AGG + chr4.3:19472666-19472685 None:intergenic 45.0%
TCTCTAACCAAACCAAGCTC+AGG + chr4.3:19472057-19472076 None:intergenic 45.0%
TGTCCACCAACACCTATAGT+AGG + chr4.3:19471595-19471614 None:intergenic 45.0%
! CACCCGGAAAACCAAGATTT+TGG + chr4.3:19471564-19471583 None:intergenic 45.0%
! TGATGGTGCCAAAGATGCTA+TGG - chr4.3:19472263-19472282 MS.gene28727:CDS 45.0%
! TTTTGGGCTATTAGAGGTGG+TGG - chr4.3:19471742-19471761 MS.gene28727:CDS 45.0%
!! CAAAATCTTGGTTTTCCGGG+TGG - chr4.3:19471562-19471581 MS.gene28727:CDS 45.0%
!! TGTCCTACTATAGGTGTTGG+TGG - chr4.3:19471589-19471608 MS.gene28727:CDS 45.0%
CTGCAGATTCGAATCCGAAG+CGG - chr4.3:19471376-19471395 MS.gene28727:CDS 50.0%
AAGCGGTGGCCATGACTTTG+AGG - chr4.3:19471393-19471412 MS.gene28727:CDS 55.0%
AGCGGTGGCCATGACTTTGA+GGG - chr4.3:19471394-19471413 MS.gene28727:CDS 55.0%
AGGACCGACCCTCAAAGTCA+TGG + chr4.3:19471405-19471424 None:intergenic 55.0%
CAAAGTCATGGCCACCGCTT+CGG + chr4.3:19471393-19471412 None:intergenic 55.0%
CAGATTCGAATCCGAAGCGG+TGG - chr4.3:19471379-19471398 MS.gene28727:CDS 55.0%
CGCTTCGGATTCGAATCTGC+AGG + chr4.3:19471378-19471397 None:intergenic 55.0%
! GTGGCCATGACTTTGAGGGT+CGG - chr4.3:19471398-19471417 MS.gene28727:CDS 55.0%
! GTTGGTGGACACATCAGTGG+CGG - chr4.3:19471604-19471623 MS.gene28727:CDS 55.0%
! TGGGCTATTAGAGGTGGTGG+AGG - chr4.3:19471745-19471764 MS.gene28727:CDS 55.0%
!! GGTGTTGGTGGACACATCAG+TGG - chr4.3:19471601-19471620 MS.gene28727:CDS 55.0%
!!! GGTGGTGGAGGAGCAAGTTT+TGG - chr4.3:19471757-19471776 MS.gene28727:CDS 55.0%
CACATCAGTGGCGGCGGCTA+TGG - chr4.3:19471613-19471632 MS.gene28727:CDS 65.0%
GGTGGACACATCAGTGGCGG+CGG - chr4.3:19471607-19471626 MS.gene28727:CDS 65.0%
TGGCGGCGGCTATGGAACCT+TGG - chr4.3:19471621-19471640 MS.gene28727:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19471067 19472704 19471067 ID=MS.gene28727
chr4.3 mRNA 19471067 19472704 19471067 ID=MS.gene28727.t1;Parent=MS.gene28727
chr4.3 exon 19471067 19472704 19471067 ID=MS.gene28727.t1.exon1;Parent=MS.gene28727.t1
chr4.3 CDS 19471067 19472704 19471067 ID=cds.MS.gene28727.t1;Parent=MS.gene28727.t1
Gene Sequence

>MS.gene28727

ATGATGCCTCTAAACTTATATTTTACTATTGTACTAATTGCTCTCCTATTTTCATATACATCTTCTGCAATTGATACTCGTACACATGAAAAAAATTTCCTTCAGTGTCTCTATAGTTATTCACATAACTCAACTTCATTCTCTGAAGTTGTGTACACCAAAGCCAATTCCTCTTACTCATCAATACTCAAATTTACAACTCAAAATCTCAGATTTGCATCCAACACAACACCTAAACCTCTTGTCATTATCACACCTAAACAGATTTCCCATATTCAAACAACCATAATTTGTTCACAAATCCATAACCTGCAGATTCGAATCCGAAGCGGTGGCCATGACTTTGAGGGTCGGTCCTATGTTTCTGAAGTCCCATTTATCATCCTTGATCTCACAAACTTCAGAGAAATTGAAGTAGATGTAGAAAGCAGAACAGCTTGGGTTCAATCTGGTGCAACAATTGGTGAACTTTACTACACAATTTACCGAAAAAACCAAAATCTTGGTTTTCCGGGTGGTGAATGTCCTACTATAGGTGTTGGTGGACACATCAGTGGCGGCGGCTATGGAACCTTGGTTCGTAAATTCGGTCTTGCAGCTGATAATATAATTGATGCTCCCATAATTGATGTGAAGGGTAGGTTTCTTGACAGAGAAGCAATGGGTGAAGATTTGTTTTGGGCTATTAGAGGTGGTGGAGGAGCAAGTTTTGGAGTCATAATTTCATGGAAGATTAAACTTGTTCAAGTTCCATCAATTGTGACAGTCTTTAATGTTCCAAAAACATTGGAACAAAATGCAACAAAGCTTATTCACAAGTGGCAGTTTTTGACAAGTAGAATTGATGAAAACTTAGAAATTACTGTTATCTTACAAAGGGTAAATTCAAGTATAAAGGGGAAGTCAAAGTCAACAGTACAAGCTATATTTCAAGCCTTGTTTCTTGGAGGTGTAGATAAGCTCATACACTTGATGCAAGAGAAGTTTCCTGAGCTTGGTTTGGTTAGAGAAGATTGTGTTGAGATGAGTTGGGTTGAATCGGTTCTTTACCTTTATGGGTTTCCGAAAGACGAACCACTTGAAACGTTGCTCAACAGAACACTAGCTGCAAAGGATATTTACAAAGTAAAATCTGATTTTGTCAAAATTCCCATTCCTGAAATTGGATTAGAAGGAATATGGCCTATGTTTCATGATGATGGTGCCAAAGATGCTATGGTGATATTTTTTCCTTATGGTGGCATAATGGATAGAATTTTTGAGTCTGAAATTCCATTTCCACATAGATATGGAAATTTATACCAAATTTATTACTCAGTTCATTGGCATCAAGAAAGTGATGAGGTGGAAAAAATGAAAATGAATTGGATTAGAAAACTTTATAGTTATATGGAACCTTTTGTTTCAAAGTCACCAAGAGCTGCATATATCAATTATAGAGATCTTGATATTGGGGTGAATAATAACAATGGTTACACAAGTTATAAGCAAGCTAGTATTTGGGGTGTTAAGTACTTCAAGAACAACTTCAAAAGATTGGTAAAAGTGAAGACTAAAGTTGATCCTCTTAACTTCTTCAGAAATGAACAGAGTATACCTTCTCTCATGTGTTCAAGGGACAAAGAAATCAGCAGCTAA
Protein sequence

>MS.gene28727.t1

MMPLNLYFTIVLIALLFSYTSSAIDTRTHEKNFLQCLYSYSHNSTSFSEVVYTKANSSYSSILKFTTQNLRFASNTTPKPLVIITPKQISHIQTTIICSQIHNLQIRIRSGGHDFEGRSYVSEVPFIILDLTNFREIEVDVESRTAWVQSGATIGELYYTIYRKNQNLGFPGGECPTIGVGGHISGGGYGTLVRKFGLAADNIIDAPIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIISWKIKLVQVPSIVTVFNVPKTLEQNATKLIHKWQFLTSRIDENLEITVILQRVNSSIKGKSKSTVQAIFQALFLGGVDKLIHLMQEKFPELGLVREDCVEMSWVESVLYLYGFPKDEPLETLLNRTLAAKDIYKVKSDFVKIPIPEIGLEGIWPMFHDDGAKDAMVIFFPYGGIMDRIFESEIPFPHRYGNLYQIYYSVHWHQESDEVEKMKMNWIRKLYSYMEPFVSKSPRAAYINYRDLDIGVNNNNGYTSYKQASIWGVKYFKNNFKRLVKVKTKVDPLNFFRNEQSIPSLMCSRDKEISS