AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene02146

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02146.t1 MTR_2g031390 98.165 545 10 0 1 545 1 545 0.0 1119
MS.gene02146.t1 MTR_4g094518 79.802 505 102 0 27 531 28 532 0.0 827
MS.gene02146.t1 MTR_4g094520 70.621 531 155 1 1 531 5 534 0.0 768
MS.gene02146.t1 MTR_4g094488 53.008 532 235 7 8 527 7 535 0.0 565
MS.gene02146.t1 MTR_3g009760 53.788 528 232 7 7 529 2 522 0.0 563
MS.gene02146.t1 MTR_4g094492 52.150 535 242 7 6 529 5 536 0.0 563
MS.gene02146.t1 MTR_2g031560 50.954 524 251 5 9 529 3 523 0.0 560
MS.gene02146.t1 MTR_2g031590 51.625 523 246 4 13 530 13 533 0.0 558
MS.gene02146.t1 MTR_4g094495 51.776 535 242 7 7 527 6 538 0.0 557
MS.gene02146.t1 MTR_3g009820 53.030 528 236 7 7 529 2 522 0.0 556
MS.gene02146.t1 MTR_4g094480 51.601 531 244 7 7 527 6 533 0.0 550
MS.gene02146.t1 MTR_4g094478 50.933 536 252 6 6 532 5 538 0.0 550
MS.gene02146.t1 MTR_3g009880 52.481 524 238 7 9 529 5 520 0.0 548
MS.gene02146.t1 MTR_4g094498 52.684 503 232 3 32 533 41 538 0.0 542
MS.gene02146.t1 MTR_2g031460 50.838 537 254 6 7 539 6 536 0.0 540
MS.gene02146.t1 MTR_2g031420 51.613 527 246 5 7 529 6 527 0.0 537
MS.gene02146.t1 MTR_4g094418 55.835 437 183 5 101 529 1 435 0.0 524
MS.gene02146.t1 MTR_2g031510 49.027 514 255 4 21 530 31 541 2.03e-172 498
MS.gene02146.t1 MTR_2g031490 48.289 526 262 6 11 530 18 539 2.85e-171 496
MS.gene02146.t1 MTR_4g094515 48.876 534 264 5 3 530 621 1151 3.18e-171 515
MS.gene02146.t1 MTR_4g094515 48.828 512 255 4 25 532 35 543 3.57e-161 489
MS.gene02146.t1 MTR_4g091150 49.112 507 250 7 28 529 37 540 8.33e-168 487
MS.gene02146.t1 MTR_4g091200 46.947 524 261 8 10 527 12 524 8.56e-166 481
MS.gene02146.t1 MTR_6g017205 45.794 535 277 7 9 539 11 536 3.55e-164 478
MS.gene02146.t1 MTR_4g057990 47.013 519 263 7 12 527 11 520 1.91e-163 475
MS.gene02146.t1 MTR_4g091190 46.756 524 266 7 9 527 11 526 1.93e-163 475
MS.gene02146.t1 MTR_4g088355 45.850 506 263 8 28 527 31 531 4.65e-157 459
MS.gene02146.t1 MTR_4g091170 45.952 457 219 7 76 527 107 540 7.89e-150 442
MS.gene02146.t1 MTR_2g023590 44.776 469 245 6 65 527 4 464 1.01e-143 423
MS.gene02146.t1 MTR_1g076830 41.055 531 298 7 16 538 21 544 1.63e-132 397
MS.gene02146.t1 MTR_3g009850 55.263 228 100 2 304 529 1 228 1.85e-85 265
MS.gene02146.t1 MTR_2g031600 52.790 233 108 1 13 245 14 244 7.69e-79 253
MS.gene02146.t1 MTR_2g031600 51.592 157 75 1 375 530 245 401 2.53e-52 184
MS.gene02146.t1 MTR_2g084370 45.946 111 38 2 127 237 1 89 1.31e-27 106
MS.gene02146.t1 MTR_8g062280 51.546 97 19 1 430 526 4 72 7.88e-26 101
MS.gene02146.t1 MTR_2g084405 51.899 79 38 0 127 205 1 79 1.93e-20 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene02146.t1 AT1G30760 67.984 506 156 2 24 527 47 548 0.0 720
MS.gene02146.t1 AT1G30760 67.984 506 156 2 24 527 29 530 0.0 718
MS.gene02146.t1 AT2G34790 66.281 519 168 3 11 527 15 528 0.0 713
MS.gene02146.t1 AT5G44400 63.873 537 182 7 2 529 3 536 0.0 685
MS.gene02146.t1 AT5G44390 60.714 532 199 7 7 531 14 542 0.0 652
MS.gene02146.t1 AT5G44380 59.417 515 201 5 21 530 69 580 0.0 610
MS.gene02146.t1 AT5G44380 59.417 515 201 5 21 530 29 540 0.0 608
MS.gene02146.t1 AT5G44360 49.609 512 242 8 27 531 30 532 3.91e-172 498
MS.gene02146.t1 AT5G44360 49.609 512 242 8 27 531 46 548 4.82e-172 498
MS.gene02146.t1 AT1G30700 49.109 505 252 4 28 530 26 527 2.45e-171 495
MS.gene02146.t1 AT4G20840 49.705 509 240 7 26 527 34 533 2.05e-168 488
MS.gene02146.t1 AT1G11770 46.816 534 265 8 9 530 4 530 5.58e-167 484
MS.gene02146.t1 AT4G20820 47.036 523 261 6 10 529 17 526 1.15e-166 483
MS.gene02146.t1 AT4G20860 48.054 514 254 7 23 529 22 529 7.88e-166 481
MS.gene02146.t1 AT1G30710 44.527 539 278 9 1 529 1 528 2.23e-159 465
MS.gene02146.t1 AT1G26380 45.627 526 273 8 8 529 9 525 4.42e-159 464
MS.gene02146.t1 AT1G34575 44.847 524 272 10 9 527 11 522 6.30e-158 461
MS.gene02146.t1 AT5G44440 44.508 528 276 8 9 527 8 527 4.86e-157 459
MS.gene02146.t1 AT1G30730 45.992 524 273 7 9 527 5 523 5.53e-156 456
MS.gene02146.t1 AT5G44440 44.508 528 276 8 9 527 78 597 5.82e-156 459
MS.gene02146.t1 AT1G26420 45.369 529 277 8 9 531 5 527 5.06e-155 454
MS.gene02146.t1 AT4G20800 44.622 502 273 4 28 528 27 524 5.22e-154 451
MS.gene02146.t1 AT1G26390 46.245 506 265 5 29 531 28 529 6.78e-154 451
MS.gene02146.t1 AT5G44410 45.966 533 259 10 9 527 10 527 1.18e-153 450
MS.gene02146.t1 AT4G20830 47.379 515 255 7 22 529 31 536 4.94e-153 449
MS.gene02146.t1 AT4G20830 46.617 532 266 8 22 544 31 553 5.33e-153 450
MS.gene02146.t1 AT1G30740 46.449 521 267 7 16 530 18 532 1.38e-152 447
MS.gene02146.t1 AT1G30740 46.449 521 267 7 16 530 15 529 1.63e-152 447
MS.gene02146.t1 AT1G01980 47.013 519 256 9 23 530 25 535 2.14e-152 447
MS.gene02146.t1 AT1G30720 45.525 514 272 6 17 527 16 524 3.97e-152 446
MS.gene02146.t1 AT1G26400 44.023 527 286 6 9 531 5 526 1.04e-150 442
MS.gene02146.t1 AT1G26410 44.094 508 269 7 30 531 53 551 8.26e-147 434
MS.gene02146.t1 AT2G34810 44.186 516 275 8 23 529 28 539 4.22e-144 426

Find 101 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCCTTGTAAACCGGTTTC+TGG 0.188434 2.2:-57276904 None:intergenic
AGCACAAAATCTAAGGTATT+TGG 0.215393 2.2:+57275943 MS.gene02146:CDS
AAGATAGTCCTTTGATGATT+TGG 0.231971 2.2:+57276947 MS.gene02146:CDS
TATGAAAGGTGTTTCAATTT+CGG 0.256742 2.2:-57276110 None:intergenic
GTGAATATAAATTCGGGTTT+TGG 0.262016 2.2:-57275977 None:intergenic
TATTCTTGATAGAAAATCTA+TGG 0.264794 2.2:+57276393 MS.gene02146:CDS
TGCATCTTGTTCTAAGCTTT+TGG 0.266276 2.2:-57276530 None:intergenic
AAATTCAGTATTTAACTCTT+TGG 0.286256 2.2:+57277046 MS.gene02146:CDS
GCTTTATACAAGCAACTTCT+TGG 0.288409 2.2:+57277217 MS.gene02146:CDS
ATTCTTCAGCACAAAATCTA+AGG 0.299578 2.2:+57275936 MS.gene02146:CDS
GCTGGTCTTTGCACAAGTTT+AGG 0.303745 2.2:+57276265 MS.gene02146:CDS
AAACCGGTTTACAAGGATTA+TGG 0.306773 2.2:+57276908 MS.gene02146:CDS
GGTGGTGGAGGTGGAAGTTT+TGG 0.306896 2.2:+57276442 MS.gene02146:CDS
AACATTTACATCGCGAAGTT+TGG 0.307697 2.2:-57276146 None:intergenic
TAACGGTGTGAATATAAATT+CGG 0.331566 2.2:-57275984 None:intergenic
ACTTCAGGTGGTGTATCATT+TGG 0.350972 2.2:-57276811 None:intergenic
AACTATAGAGATCTTGATTT+GGG 0.358282 2.2:+57277171 MS.gene02146:CDS
CATGAGAAAAGTGATGTTCA+TGG 0.362940 2.2:+57276235 MS.gene02146:CDS
TGTCTAGTCAAACCTAATTC+AGG 0.362943 2.2:-57276736 None:intergenic
AAATGCAAGAGATGGGAATA+AGG 0.364388 2.2:+57277372 MS.gene02146:CDS
CTTTATACAAGCAACTTCTT+GGG 0.364579 2.2:+57277218 MS.gene02146:CDS
TTTAGTTGCATAGTTGAAAC+TGG 0.369940 2.2:-57277342 None:intergenic
GGATCCATGATGAGAAAATA+TGG 0.381854 2.2:+57276319 MS.gene02146:CDS
GTTCCATTTCTGTGAGGAAA+AGG 0.384120 2.2:-57277015 None:intergenic
AAATCTGTTATGTATATTGC+AGG 0.387126 2.2:+57276784 MS.gene02146:CDS
GGTGTTGGTGGACACATAAC+AGG 0.389612 2.2:+57276286 MS.gene02146:CDS
GAACTATAGAGATCTTGATT+TGG 0.391233 2.2:+57277170 MS.gene02146:CDS
GTGTAAATTGATGCATAAAA+TGG 0.401588 2.2:-57275881 None:intergenic
GATGTTCATGGTTTCCCTGC+TGG 0.402996 2.2:+57276247 MS.gene02146:CDS
TTGTTCATCATGCCACCATA+TGG 0.406387 2.2:-57276973 None:intergenic
CAAAGCATGTTGATTGGATC+AGG 0.408035 2.2:+57277091 MS.gene02146:CDS
AGGATTATGGCAAAGGTTGT+TGG 0.408844 2.2:+57276921 MS.gene02146:CDS
ATGCTTCAAAGCATGTTGAT+TGG 0.408891 2.2:+57277085 MS.gene02146:CDS
CAAAGATAATTTCAATAGGT+TGG 0.410828 2.2:+57277254 MS.gene02146:CDS
CTTGGAAACTTAGACACATA+AGG 0.417454 2.2:-57277135 None:intergenic
GCTATTAGAGGTGGTGGAGG+TGG 0.420433 2.2:+57276433 MS.gene02146:CDS
TTTCCTTCAAGCAAAACTTC+AGG 0.421604 2.2:-57276826 None:intergenic
TATTTAACTCTTTGGCAAGA+TGG 0.424710 2.2:+57277054 MS.gene02146:CDS
TTGAACAAGTAACAGCTACT+TGG 0.425622 2.2:-57276021 None:intergenic
CACATAACAGGAGGTGCATA+TGG 0.426699 2.2:+57276298 MS.gene02146:CDS
TGTAAACCGGTTTCTGGTAT+CGG 0.429621 2.2:-57276898 None:intergenic
AACTTTCCTTCATAACTTGA+AGG 0.432029 2.2:-57276711 None:intergenic
ATTTCAAAGATAATTTCAAT+AGG 0.435565 2.2:+57277250 MS.gene02146:CDS
AACGGTGTGAATATAAATTC+GGG 0.438763 2.2:-57275983 None:intergenic
CAAGTAAGATTGTTCATAGA+TGG 0.444999 2.2:+57276551 MS.gene02146:CDS
TTGGAAACTTAGACACATAA+GGG 0.449567 2.2:-57277134 None:intergenic
TTGCCATAATCCTTGTAAAC+CGG 0.455650 2.2:-57276911 None:intergenic
TGGATCCTGAAAATGTGTTC+AGG 0.457657 2.2:+57277295 MS.gene02146:CDS
TCTTACAATGCTCAATTCCT+AGG 0.460457 2.2:+57276673 MS.gene02146:CDS
TGGGCTATTAGAGGTGGTGG+AGG 0.463852 2.2:+57276430 MS.gene02146:CDS
ATACCAGAAACCGGTTTACA+AGG 0.466241 2.2:+57276901 MS.gene02146:CDS
TGGACATGTGAATCGGTTAA+CGG 0.471684 2.2:-57276001 None:intergenic
ATGATGAGAAAATATGGTCT+TGG 0.474676 2.2:+57276325 MS.gene02146:CDS
CTGAATAGAAACTTAGACAT+TGG 0.484197 2.2:-57275847 None:intergenic
TTGATGATTTGGAATCCATA+TGG 0.487109 2.2:+57276958 MS.gene02146:CDS
AAAATTGCCAAGTGTGAACT+TGG 0.491421 2.2:-57275767 None:intergenic
GTTACTTGTTCAAAGAAACT+TGG 0.495838 2.2:+57276031 MS.gene02146:CDS
AAACTTGTGCAAAGACCAGC+AGG 0.496208 2.2:-57276262 None:intergenic
ATGAGAGTCCTTCATAATCA+TGG 0.497445 2.2:-57276081 None:intergenic
AAAACTCCTTCAAGTTATGA+AGG 0.498104 2.2:+57276705 MS.gene02146:CDS
TTGTTTGTAGCACTAGCAGC+TGG 0.499020 2.2:-57276628 None:intergenic
TGCACAAGTTTAGGTGTTGG+TGG 0.501953 2.2:+57276274 MS.gene02146:CDS
TAGTTCACATATGCTTCCCT+TGG 0.502365 2.2:-57277153 None:intergenic
TTCCATTTCTGTGAGGAAAA+GGG 0.505141 2.2:-57277014 None:intergenic
GCGAAAATAATCGACGCAAA+CGG 0.505268 2.2:+57276367 MS.gene02146:CDS
AAGACAAGAAATGCAAGAGA+TGG 0.506067 2.2:+57277364 MS.gene02146:CDS
ATTCTTGATAGAAAATCTAT+GGG 0.512123 2.2:+57276394 MS.gene02146:CDS
AGCTACTTGGACATGTGAAT+CGG 0.521216 2.2:-57276008 None:intergenic
TTAATGAAATTGATATCAAG+AGG 0.521425 2.2:-57275793 None:intergenic
AAGATTGCATGGAAACTAGC+TGG 0.524173 2.2:+57276758 MS.gene02146:CDS
GGTGAAAATTAAGACTAAAG+TGG 0.529045 2.2:+57277275 MS.gene02146:CDS
CTTTGCACAAGTTTAGGTGT+TGG 0.529715 2.2:+57276271 MS.gene02146:CDS
CTTATGTGTCTAAGTTTCCA+AGG 0.535444 2.2:+57277136 MS.gene02146:CDS
TTTATACAAGCAACTTCTTG+GGG 0.537380 2.2:+57277219 MS.gene02146:CDS
ATATCGAAGATAATAGCGCT+TGG 0.537584 2.2:+57276170 MS.gene02146:CDS
GTTTACAAGGATTATGGCAA+AGG 0.546077 2.2:+57276914 MS.gene02146:CDS
ATCTTCACCAAGTTCACACT+TGG 0.548533 2.2:+57275760 MS.gene02146:CDS
TCCTGTTCCGAAAACCGTGA+CGG 0.550690 2.2:+57276495 MS.gene02146:CDS
CCTTCAAGCAAAACTTCAGG+TGG 0.556110 2.2:-57276823 None:intergenic
AGACAAGAAATGCAAGAGAT+GGG 0.558462 2.2:+57277365 MS.gene02146:CDS
ATTCAAGCCGGTGCTACTAT+CGG 0.565892 2.2:+57276193 MS.gene02146:CDS
AGAGAGCCGATACCAGAAAC+CGG 0.570525 2.2:+57276892 MS.gene02146:CDS
CACTTCGCCGATAGTAGCAC+CGG 0.580226 2.2:-57276200 None:intergenic
TTACATCGCGAAGTTTGGCT+AGG 0.583149 2.2:-57276141 None:intergenic
TATGGATTCCAAATCATCAA+AGG 0.588783 2.2:-57276955 None:intergenic
AGTGGTGGCCATGATTATGA+AGG 0.592283 2.2:+57276073 MS.gene02146:CDS
TTTGACTAGACAAGATTGCA+TGG 0.592317 2.2:+57276747 MS.gene02146:CDS
CATCTAAGAGTAAGAAGTGG+TGG 0.612712 2.2:+57276058 MS.gene02146:CDS
TTATGTGTCTAAGTTTCCAA+GGG 0.614808 2.2:+57277137 MS.gene02146:CDS
GCTAGGTCGACGATTATGAA+AGG 0.622843 2.2:-57276124 None:intergenic
TGCATAGTTGAAACTGGAAG+TGG 0.634647 2.2:-57277336 None:intergenic
ATGATTTGGAATCCATATGG+TGG 0.656424 2.2:+57276961 MS.gene02146:CDS
AATAGCGCTTGGATTCAAGC+CGG 0.660237 2.2:+57276181 MS.gene02146:CDS
ATTCATCTAAGAGTAAGAAG+TGG 0.668072 2.2:+57276055 MS.gene02146:CDS
TAAAGTGTTCCATTTCTGTG+AGG 0.668658 2.2:-57277021 None:intergenic
TTCTTGATAGAAAATCTATG+GGG 0.690602 2.2:+57276395 MS.gene02146:CDS
TGGAAACTTAGACACATAAG+GGG 0.690609 2.2:-57277133 None:intergenic
ATAGTTGAAACTGGAAGTGG+TGG 0.697983 2.2:-57277333 None:intergenic
TCTTGATAGAAAATCTATGG+GGG 0.706423 2.2:+57276396 MS.gene02146:CDS
GTTGGTGGACACATAACAGG+AGG 0.719255 2.2:+57276289 MS.gene02146:CDS
AACTTGTGCAAAGACCAGCA+GGG 0.736490 2.2:-57276261 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCAAAGATAATTTCAAT+AGG + chr2.2:57277250-57277269 MS.gene02146:CDS 15.0%
!! AAATTCAGTATTTAACTCTT+TGG + chr2.2:57277046-57277065 MS.gene02146:CDS 20.0%
!! ATTCTTGATAGAAAATCTAT+GGG + chr2.2:57276394-57276413 MS.gene02146:CDS 20.0%
!! TATTCTTGATAGAAAATCTA+TGG + chr2.2:57276393-57276412 MS.gene02146:CDS 20.0%
!! TTAATGAAATTGATATCAAG+AGG - chr2.2:57275796-57275815 None:intergenic 20.0%
! AAATCTGTTATGTATATTGC+AGG + chr2.2:57276784-57276803 MS.gene02146:CDS 25.0%
! AACTATAGAGATCTTGATTT+GGG + chr2.2:57277171-57277190 MS.gene02146:CDS 25.0%
! CAAAGATAATTTCAATAGGT+TGG + chr2.2:57277254-57277273 MS.gene02146:CDS 25.0%
! GTGTAAATTGATGCATAAAA+TGG - chr2.2:57275884-57275903 None:intergenic 25.0%
! TAACGGTGTGAATATAAATT+CGG - chr2.2:57275987-57276006 None:intergenic 25.0%
! TTCTTGATAGAAAATCTATG+GGG + chr2.2:57276395-57276414 MS.gene02146:CDS 25.0%
!! AAGAGATTTTCATCAATAGT+TGG - chr2.2:57276589-57276608 None:intergenic 25.0%
!!! TATGAAAGGTGTTTCAATTT+CGG - chr2.2:57276113-57276132 None:intergenic 25.0%
AACGGTGTGAATATAAATTC+GGG - chr2.2:57275986-57276005 None:intergenic 30.0%
AACTTTCCTTCATAACTTGA+AGG - chr2.2:57276714-57276733 None:intergenic 30.0%
ATGATGAGAAAATATGGTCT+TGG + chr2.2:57276325-57276344 MS.gene02146:CDS 30.0%
ATTCATCTAAGAGTAAGAAG+TGG + chr2.2:57276055-57276074 MS.gene02146:CDS 30.0%
ATTCTTCAGCACAAAATCTA+AGG + chr2.2:57275936-57275955 MS.gene02146:CDS 30.0%
CAAGTAAGATTGTTCATAGA+TGG + chr2.2:57276551-57276570 MS.gene02146:CDS 30.0%
CTGAATAGAAACTTAGACAT+TGG - chr2.2:57275850-57275869 None:intergenic 30.0%
CTTTATACAAGCAACTTCTT+GGG + chr2.2:57277218-57277237 MS.gene02146:CDS 30.0%
GAACTATAGAGATCTTGATT+TGG + chr2.2:57277170-57277189 MS.gene02146:CDS 30.0%
GGTGAAAATTAAGACTAAAG+TGG + chr2.2:57277275-57277294 MS.gene02146:CDS 30.0%
GTTACTTGTTCAAAGAAACT+TGG + chr2.2:57276031-57276050 MS.gene02146:CDS 30.0%
TATGGATTCCAAATCATCAA+AGG - chr2.2:57276958-57276977 None:intergenic 30.0%
TATTTAACTCTTTGGCAAGA+TGG + chr2.2:57277054-57277073 MS.gene02146:CDS 30.0%
TCTTGATAGAAAATCTATGG+GGG + chr2.2:57276396-57276415 MS.gene02146:CDS 30.0%
TTATGTGTCTAAGTTTCCAA+GGG + chr2.2:57277137-57277156 MS.gene02146:CDS 30.0%
TTGGAAACTTAGACACATAA+GGG - chr2.2:57277137-57277156 None:intergenic 30.0%
TTTAGTTGCATAGTTGAAAC+TGG - chr2.2:57277345-57277364 None:intergenic 30.0%
TTTATACAAGCAACTTCTTG+GGG + chr2.2:57277219-57277238 MS.gene02146:CDS 30.0%
! AAAACTCCTTCAAGTTATGA+AGG + chr2.2:57276705-57276724 MS.gene02146:CDS 30.0%
! AAGACCATATTTTCTCATCA+TGG - chr2.2:57276326-57276345 None:intergenic 30.0%
! AAGATAGTCCTTTGATGATT+TGG + chr2.2:57276947-57276966 MS.gene02146:CDS 30.0%
! AAGGAAAGTTTTCCTGAATT+AGG + chr2.2:57276724-57276743 MS.gene02146:CDS 30.0%
! AGCACAAAATCTAAGGTATT+TGG + chr2.2:57275943-57275962 MS.gene02146:CDS 30.0%
! GATTTTGTGCTGAAGAATTA+AGG - chr2.2:57275934-57275953 None:intergenic 30.0%
! TGGCATGATGAACAATTTTT+CGG + chr2.2:57276981-57277000 MS.gene02146:CDS 30.0%
! TTGATGATTTGGAATCCATA+TGG + chr2.2:57276958-57276977 MS.gene02146:CDS 30.0%
!! GAAGTTTTGGTATTCTTCTT+TGG + chr2.2:57276455-57276474 MS.gene02146:CDS 30.0%
!! GTGAATATAAATTCGGGTTT+TGG - chr2.2:57275980-57275999 None:intergenic 30.0%
!! TTGTTCTAAGCTTTTGGTAA+CGG - chr2.2:57276527-57276546 None:intergenic 30.0%
AAAATTGCCAAGTGTGAACT+TGG - chr2.2:57275770-57275789 None:intergenic 35.0%
AACATTTACATCGCGAAGTT+TGG - chr2.2:57276149-57276168 None:intergenic 35.0%
AAGACAAGAAATGCAAGAGA+TGG + chr2.2:57277364-57277383 MS.gene02146:CDS 35.0%
AGACAAGAAATGCAAGAGAT+GGG + chr2.2:57277365-57277384 MS.gene02146:CDS 35.0%
ATATCGAAGATAATAGCGCT+TGG + chr2.2:57276170-57276189 MS.gene02146:CDS 35.0%
ATGAGAGTCCTTCATAATCA+TGG - chr2.2:57276084-57276103 None:intergenic 35.0%
ATGCTTCAAAGCATGTTGAT+TGG + chr2.2:57277085-57277104 MS.gene02146:CDS 35.0%
CATGAGAAAAGTGATGTTCA+TGG + chr2.2:57276235-57276254 MS.gene02146:CDS 35.0%
CTTATGTGTCTAAGTTTCCA+AGG + chr2.2:57277136-57277155 MS.gene02146:CDS 35.0%
CTTGGAAACTTAGACACATA+AGG - chr2.2:57277138-57277157 None:intergenic 35.0%
GCTTTATACAAGCAACTTCT+TGG + chr2.2:57277217-57277236 MS.gene02146:CDS 35.0%
GGATCCATGATGAGAAAATA+TGG + chr2.2:57276319-57276338 MS.gene02146:CDS 35.0%
GTTTACAAGGATTATGGCAA+AGG + chr2.2:57276914-57276933 MS.gene02146:CDS 35.0%
TCTTACAATGCTCAATTCCT+AGG + chr2.2:57276673-57276692 MS.gene02146:CDS 35.0%
TGGAAACTTAGACACATAAG+GGG - chr2.2:57277136-57277155 None:intergenic 35.0%
TGTCTAGTCAAACCTAATTC+AGG - chr2.2:57276739-57276758 None:intergenic 35.0%
TTCCATTTCTGTGAGGAAAA+GGG - chr2.2:57277017-57277036 None:intergenic 35.0%
TTGAACAAGTAACAGCTACT+TGG - chr2.2:57276024-57276043 None:intergenic 35.0%
TTGCCATAATCCTTGTAAAC+CGG - chr2.2:57276914-57276933 None:intergenic 35.0%
TTTCCTTCAAGCAAAACTTC+AGG - chr2.2:57276829-57276848 None:intergenic 35.0%
TTTGACTAGACAAGATTGCA+TGG + chr2.2:57276747-57276766 MS.gene02146:CDS 35.0%
! AAACCGGTTTACAAGGATTA+TGG + chr2.2:57276908-57276927 MS.gene02146:CDS 35.0%
! ATGATTTGGAATCCATATGG+TGG + chr2.2:57276961-57276980 MS.gene02146:CDS 35.0%
! GAATTCTTTTGGGCTATTAG+AGG + chr2.2:57276421-57276440 MS.gene02146:CDS 35.0%
! TAAAGTGTTCCATTTCTGTG+AGG - chr2.2:57277024-57277043 None:intergenic 35.0%
!! GTTTTGGTATTCTTCTTTGG+TGG + chr2.2:57276458-57276477 MS.gene02146:CDS 35.0%
!! TGCATCTTGTTCTAAGCTTT+TGG - chr2.2:57276533-57276552 None:intergenic 35.0%
AAGATTGCATGGAAACTAGC+TGG + chr2.2:57276758-57276777 MS.gene02146:CDS 40.0%
ACTTCAGGTGGTGTATCATT+TGG - chr2.2:57276814-57276833 None:intergenic 40.0%
AGCTACTTGGACATGTGAAT+CGG - chr2.2:57276011-57276030 None:intergenic 40.0%
AGGATTATGGCAAAGGTTGT+TGG + chr2.2:57276921-57276940 MS.gene02146:CDS 40.0%
ATAGTTGAAACTGGAAGTGG+TGG - chr2.2:57277336-57277355 None:intergenic 40.0%
ATCTTCACCAAGTTCACACT+TGG + chr2.2:57275760-57275779 MS.gene02146:CDS 40.0%
CATCTAAGAGTAAGAAGTGG+TGG + chr2.2:57276058-57276077 MS.gene02146:CDS 40.0%
CTTTGCACAAGTTTAGGTGT+TGG + chr2.2:57276271-57276290 MS.gene02146:CDS 40.0%
GCGAAAATAATCGACGCAAA+CGG + chr2.2:57276367-57276386 MS.gene02146:CDS 40.0%
GTTCCATTTCTGTGAGGAAA+AGG - chr2.2:57277018-57277037 None:intergenic 40.0%
TAGTTCACATATGCTTCCCT+TGG - chr2.2:57277156-57277175 None:intergenic 40.0%
TGATAGAAAATCTATGGGGG+AGG + chr2.2:57276399-57276418 MS.gene02146:CDS 40.0%
TGCATAGTTGAAACTGGAAG+TGG - chr2.2:57277339-57277358 None:intergenic 40.0%
TGGACATGTGAATCGGTTAA+CGG - chr2.2:57276004-57276023 None:intergenic 40.0%
TGGATCCTGAAAATGTGTTC+AGG + chr2.2:57277295-57277314 MS.gene02146:CDS 40.0%
TTGTTCATCATGCCACCATA+TGG - chr2.2:57276976-57276995 None:intergenic 40.0%
! AATCCTTGTAAACCGGTTTC+TGG - chr2.2:57276907-57276926 None:intergenic 40.0%
! ATACCAGAAACCGGTTTACA+AGG + chr2.2:57276901-57276920 MS.gene02146:CDS 40.0%
! ATCCCTTTTCCTCACAGAAA+TGG + chr2.2:57277012-57277031 MS.gene02146:CDS 40.0%
! CAAAGCATGTTGATTGGATC+AGG + chr2.2:57277091-57277110 MS.gene02146:CDS 40.0%
! GGAGTTTTTCTGAATCACCT+AGG - chr2.2:57276693-57276712 None:intergenic 40.0%
! GTAAAAACCGTCACGGTTTT+CGG - chr2.2:57276505-57276524 None:intergenic 40.0%
! TATGGGGGAGGAATTCTTTT+GGG + chr2.2:57276411-57276430 MS.gene02146:CDS 40.0%
! TCATGCCTGAACACATTTTC+AGG - chr2.2:57277303-57277322 None:intergenic 40.0%
! TTCTTTTGGGCTATTAGAGG+TGG + chr2.2:57276424-57276443 MS.gene02146:CDS 40.0%
!! TGTAAACCGGTTTCTGGTAT+CGG - chr2.2:57276901-57276920 None:intergenic 40.0%
AAACTTGTGCAAAGACCAGC+AGG - chr2.2:57276265-57276284 None:intergenic 45.0%
AACTTGTGCAAAGACCAGCA+GGG - chr2.2:57276264-57276283 None:intergenic 45.0%
AATAGCGCTTGGATTCAAGC+CGG + chr2.2:57276181-57276200 MS.gene02146:CDS 45.0%
AGTGGTGGCCATGATTATGA+AGG + chr2.2:57276073-57276092 MS.gene02146:CDS 45.0%
CCTTCAAGCAAAACTTCAGG+TGG - chr2.2:57276826-57276845 None:intergenic 45.0%
GCTAGGTCGACGATTATGAA+AGG - chr2.2:57276127-57276146 None:intergenic 45.0%
TTACATCGCGAAGTTTGGCT+AGG - chr2.2:57276144-57276163 None:intergenic 45.0%
TTGTTTGTAGCACTAGCAGC+TGG - chr2.2:57276631-57276650 None:intergenic 45.0%
! CACATAACAGGAGGTGCATA+TGG + chr2.2:57276298-57276317 MS.gene02146:CDS 45.0%
! CCACCTGAAGTTTTGCTTGA+AGG + chr2.2:57276823-57276842 MS.gene02146:CDS 45.0%
! CTATGGGGGAGGAATTCTTT+TGG + chr2.2:57276410-57276429 MS.gene02146:CDS 45.0%
! GGTAACGGTAAAAACCGTCA+CGG - chr2.2:57276512-57276531 None:intergenic 45.0%
! TTTTGGGCTATTAGAGGTGG+TGG + chr2.2:57276427-57276446 MS.gene02146:CDS 45.0%
!! ATTCAAGCCGGTGCTACTAT+CGG + chr2.2:57276193-57276212 MS.gene02146:CDS 45.0%
!! GCTGGTCTTTGCACAAGTTT+AGG + chr2.2:57276265-57276284 MS.gene02146:CDS 45.0%
!! TGCACAAGTTTAGGTGTTGG+TGG + chr2.2:57276274-57276293 MS.gene02146:CDS 45.0%
!!! AATTCGGGTTTTGGAGCTGA+AGG - chr2.2:57275971-57275990 None:intergenic 45.0%
AGAGAGCCGATACCAGAAAC+CGG + chr2.2:57276892-57276911 MS.gene02146:CDS 50.0%
GATGTTCATGGTTTCCCTGC+TGG + chr2.2:57276247-57276266 MS.gene02146:CDS 50.0%
TCCTGTTCCGAAAACCGTGA+CGG + chr2.2:57276495-57276514 MS.gene02146:CDS 50.0%
! GTTGGTGGACACATAACAGG+AGG + chr2.2:57276289-57276308 MS.gene02146:CDS 50.0%
!! GGTGTTGGTGGACACATAAC+AGG + chr2.2:57276286-57276305 MS.gene02146:CDS 50.0%
CACTTCGCCGATAGTAGCAC+CGG - chr2.2:57276203-57276222 None:intergenic 55.0%
! ACCGTCACGGTTTTCGGAAC+AGG - chr2.2:57276499-57276518 None:intergenic 55.0%
! TGGGCTATTAGAGGTGGTGG+AGG + chr2.2:57276430-57276449 MS.gene02146:CDS 55.0%
!! GCTATTAGAGGTGGTGGAGG+TGG + chr2.2:57276433-57276452 MS.gene02146:CDS 55.0%
!!! GGTGGTGGAGGTGGAAGTTT+TGG + chr2.2:57276442-57276461 MS.gene02146:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 57275755 57277392 57275755 ID=MS.gene02146
chr2.2 mRNA 57275755 57277392 57275755 ID=MS.gene02146.t1;Parent=MS.gene02146
chr2.2 exon 57275755 57277392 57275755 ID=MS.gene02146.t1.exon1;Parent=MS.gene02146.t1
chr2.2 CDS 57275755 57277392 57275755 ID=cds.MS.gene02146.t1;Parent=MS.gene02146.t1
Gene Sequence

>MS.gene02146

ATGATATCTTCACCAAGTTCACACTTGGCAATTTTAATCCTCTTGATATCAATTTCATTAACAAAATCAGCTTCAATTGAAGAAAACTTTGTCCAATGTCTAAGTTTCTATTCAGACAAAGCAGCACCATTTTATGCATCAATTTACACTCAAAACAATGCTTCATTTAACAACGTCCTTAATTCTTCAGCACAAAATCTAAGGTATTTGGTACCTTCAGCTCCAAAACCCGAATTTATATTCACACCGTTAACCGATTCACATGTCCAAGTAGCTGTTACTTGTTCAAAGAAACTTGGTATTCATCTAAGAGTAAGAAGTGGTGGCCATGATTATGAAGGACTCTCATATGTTTCCGAAATTGAAACACCTTTCATAATCGTCGACCTAGCCAAACTTCGCGATGTAAATGTTGATATCGAAGATAATAGCGCTTGGATTCAAGCCGGTGCTACTATCGGCGAAGTGTATTATAGAATACATGAGAAAAGTGATGTTCATGGTTTCCCTGCTGGTCTTTGCACAAGTTTAGGTGTTGGTGGACACATAACAGGAGGTGCATATGGATCCATGATGAGAAAATATGGTCTTGGAGCTGATAATGTTCTCGATGCGAAAATAATCGACGCAAACGGCAATATTCTTGATAGAAAATCTATGGGGGAGGAATTCTTTTGGGCTATTAGAGGTGGTGGAGGTGGAAGTTTTGGTATTCTTCTTTGGTGGAAGATTAAGCTTGTTCCTGTTCCGAAAACCGTGACGGTTTTTACCGTTACCAAAAGCTTAGAACAAGATGCAAGTAAGATTGTTCATAGATGGCAAGAAGTAGCACCAACTATTGATGAAAATCTCTTTATGAGAGTTATCATTCAACCAGCTGCTAGTGCTACAAACAAAACTCAAAGAACTATTACTACTTCTTACAATGCTCAATTCCTAGGTGATTCAGAAAAACTCCTTCAAGTTATGAAGGAAAGTTTTCCTGAATTAGGTTTGACTAGACAAGATTGCATGGAAACTAGCTGGATCAAATCTGTTATGTATATTGCAGGCTATCCAAATGATACACCACCTGAAGTTTTGCTTGAAGGAAAATCAACATTCAAGAATTACTTCAAAGCAAAGTCAGATTTCGTAAGAGAGCCGATACCAGAAACCGGTTTACAAGGATTATGGCAAAGGTTGTTGGAAGAAGATAGTCCTTTGATGATTTGGAATCCATATGGTGGCATGATGAACAATTTTTCGGAATCTGATATCCCTTTTCCTCACAGAAATGGAACACTTTACAAAATTCAGTATTTAACTCTTTGGCAAGATGGAGACAAGAATGCTTCAAAGCATGTTGATTGGATCAGGAAGCTTTATAACTACATGACCCCTTATGTGTCTAAGTTTCCAAGGGAAGCATATGTGAACTATAGAGATCTTGATTTGGGAATGAATAAGAAGAATAGTACAAGCTTTATACAAGCAACTTCTTGGGGTAACATGTATTTCAAAGATAATTTCAATAGGTTGGTGAAAATTAAGACTAAAGTGGATCCTGAAAATGTGTTCAGGCATGAGCAGAGTATTCCACCACTTCCAGTTTCAACTATGCAACTAAAAGACAAGAAATGCAAGAGATGGGAATAA
Protein sequence

>MS.gene02146.t1

MISSPSSHLAILILLISISLTKSASIEENFVQCLSFYSDKAAPFYASIYTQNNASFNNVLNSSAQNLRYLVPSAPKPEFIFTPLTDSHVQVAVTCSKKLGIHLRVRSGGHDYEGLSYVSEIETPFIIVDLAKLRDVNVDIEDNSAWIQAGATIGEVYYRIHEKSDVHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGADNVLDAKIIDANGNILDRKSMGEEFFWAIRGGGGGSFGILLWWKIKLVPVPKTVTVFTVTKSLEQDASKIVHRWQEVAPTIDENLFMRVIIQPAASATNKTQRTITTSYNAQFLGDSEKLLQVMKESFPELGLTRQDCMETSWIKSVMYIAGYPNDTPPEVLLEGKSTFKNYFKAKSDFVREPIPETGLQGLWQRLLEEDSPLMIWNPYGGMMNNFSESDIPFPHRNGTLYKIQYLTLWQDGDKNASKHVDWIRKLYNYMTPYVSKFPREAYVNYRDLDLGMNKKNSTSFIQATSWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSTMQLKDKKCKRWE