Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51644.t1 | XP_003598276.1 | 88.7 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 2.70E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51644.t1 | Q9SUC6 | 52.8 | 477 | 205 | 8 | 8 | 468 | 11 | 483 | 2.9e-143 | 510.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51644.t1 | G7J0C2 | 88.7 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 1.9e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51644 | MS.gene28725 | PPI |
| MS.gene51644 | MS.gene36445 | PPI |
| MS.gene51644 | MS.gene28723 | PPI |
| MS.gene51644 | MS.gene35083 | PPI |
| MS.gene028369 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene35113 | PPI |
| MS.gene51644 | MS.gene047977 | PPI |
| MS.gene51644 | MS.gene35102 | PPI |
| MS.gene51644 | MS.gene28742 | PPI |
| MS.gene35104 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene35106 | PPI |
| MS.gene51644 | MS.gene028353 | PPI |
| MS.gene35105 | MS.gene51644 | PPI |
| MS.gene36438 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene36444 | PPI |
| MS.gene51644 | MS.gene35114 | PPI |
| MS.gene003357 | MS.gene51644 | PPI |
| MS.gene28724 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene028354 | PPI |
| MS.gene51644 | MS.gene028347 | PPI |
| MS.gene51644 | MS.gene36437 | PPI |
| MS.gene51644 | MS.gene28726 | PPI |
| MS.gene51644 | MS.gene28717 | PPI |
| MS.gene02146 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene36439 | PPI |
| MS.gene51644 | MS.gene28727 | PPI |
| MS.gene51644 | MS.gene36435 | PPI |
| MS.gene36421 | MS.gene51644 | PPI |
| MS.gene51644 | MS.gene35103 | PPI |
| MS.gene51644 | MS.gene028357 | PPI |
| MS.gene51644 | MS.gene028346 | PPI |
| MS.gene51644 | MS.gene028352 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51644.t1 | MTR_3g009760 | 88.655 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 0.0 | 848 |
| MS.gene51644.t1 | MTR_3g009820 | 88.235 | 476 | 48 | 1 | 1 | 468 | 1 | 476 | 0.0 | 840 |
| MS.gene51644.t1 | MTR_3g009880 | 84.728 | 478 | 63 | 2 | 1 | 470 | 1 | 476 | 0.0 | 806 |
| MS.gene51644.t1 | MTR_2g031390 | 50.538 | 465 | 211 | 7 | 19 | 468 | 23 | 483 | 2.34e-169 | 488 |
| MS.gene51644.t1 | MTR_2g031420 | 53.750 | 480 | 203 | 8 | 1 | 467 | 8 | 481 | 2.35e-168 | 485 |
| MS.gene51644.t1 | MTR_2g031460 | 52.795 | 483 | 205 | 8 | 1 | 468 | 8 | 482 | 7.11e-168 | 484 |
| MS.gene51644.t1 | MTR_4g094488 | 51.340 | 485 | 214 | 6 | 3 | 467 | 7 | 489 | 9.19e-167 | 481 |
| MS.gene51644.t1 | MTR_4g094495 | 52.147 | 489 | 209 | 6 | 2 | 467 | 6 | 492 | 9.98e-166 | 479 |
| MS.gene51644.t1 | MTR_4g094492 | 51.951 | 487 | 209 | 8 | 2 | 467 | 6 | 488 | 1.43e-164 | 476 |
| MS.gene51644.t1 | MTR_4g094520 | 49.585 | 482 | 224 | 9 | 1 | 468 | 10 | 486 | 1.84e-164 | 475 |
| MS.gene51644.t1 | MTR_4g094478 | 50.104 | 479 | 222 | 5 | 4 | 467 | 11 | 487 | 8.64e-164 | 474 |
| MS.gene51644.t1 | MTR_4g094518 | 53.813 | 459 | 191 | 9 | 25 | 467 | 30 | 483 | 1.43e-159 | 462 |
| MS.gene51644.t1 | MTR_4g094480 | 48.140 | 484 | 231 | 6 | 2 | 467 | 6 | 487 | 2.51e-156 | 454 |
| MS.gene51644.t1 | MTR_2g031560 | 46.362 | 481 | 243 | 5 | 4 | 472 | 3 | 480 | 1.15e-155 | 454 |
| MS.gene51644.t1 | MTR_4g094418 | 55.814 | 387 | 154 | 4 | 98 | 467 | 1 | 387 | 8.35e-155 | 453 |
| MS.gene51644.t1 | MTR_4g094498 | 48.643 | 479 | 225 | 6 | 7 | 467 | 12 | 487 | 1.85e-154 | 450 |
| MS.gene51644.t1 | MTR_2g031590 | 45.868 | 484 | 242 | 7 | 1 | 467 | 5 | 485 | 4.07e-150 | 439 |
| MS.gene51644.t1 | MTR_4g057990 | 43.789 | 475 | 251 | 5 | 1 | 465 | 4 | 472 | 2.02e-138 | 408 |
| MS.gene51644.t1 | MTR_2g031510 | 44.017 | 468 | 243 | 6 | 17 | 467 | 28 | 493 | 1.15e-137 | 407 |
| MS.gene51644.t1 | MTR_2g031490 | 43.532 | 487 | 251 | 9 | 1 | 467 | 9 | 491 | 4.60e-137 | 405 |
| MS.gene51644.t1 | MTR_4g091200 | 45.397 | 478 | 240 | 7 | 1 | 465 | 7 | 476 | 4.74e-135 | 400 |
| MS.gene51644.t1 | MTR_4g091190 | 44.676 | 479 | 236 | 9 | 7 | 465 | 9 | 478 | 5.08e-135 | 400 |
| MS.gene51644.t1 | MTR_4g091150 | 46.389 | 457 | 226 | 8 | 24 | 465 | 37 | 489 | 8.97e-134 | 397 |
| MS.gene51644.t1 | MTR_4g088355 | 45.788 | 463 | 220 | 8 | 24 | 465 | 31 | 483 | 2.11e-132 | 393 |
| MS.gene51644.t1 | MTR_4g094515 | 45.267 | 486 | 243 | 9 | 1 | 467 | 622 | 1103 | 1.16e-131 | 409 |
| MS.gene51644.t1 | MTR_4g094515 | 44.979 | 478 | 235 | 11 | 11 | 467 | 23 | 493 | 3.66e-124 | 389 |
| MS.gene51644.t1 | MTR_6g017205 | 44.118 | 476 | 241 | 8 | 7 | 465 | 9 | 476 | 1.16e-131 | 392 |
| MS.gene51644.t1 | MTR_4g091170 | 45.545 | 404 | 191 | 5 | 73 | 465 | 107 | 492 | 1.76e-125 | 377 |
| MS.gene51644.t1 | MTR_2g023590 | 46.324 | 408 | 202 | 6 | 70 | 464 | 12 | 415 | 9.16e-122 | 364 |
| MS.gene51644.t1 | MTR_3g009850 | 88.202 | 178 | 21 | 0 | 291 | 468 | 5 | 182 | 1.14e-112 | 332 |
| MS.gene51644.t1 | MTR_1g076830 | 41.686 | 427 | 234 | 6 | 54 | 466 | 60 | 485 | 1.14e-104 | 322 |
| MS.gene51644.t1 | MTR_2g031600 | 44.490 | 245 | 120 | 4 | 1 | 231 | 1 | 243 | 3.42e-62 | 208 |
| MS.gene51644.t1 | MTR_2g031600 | 51.327 | 113 | 52 | 2 | 356 | 467 | 243 | 353 | 3.84e-32 | 127 |
| MS.gene51644.t1 | MTR_2g084370 | 73.786 | 103 | 13 | 1 | 122 | 224 | 1 | 89 | 5.03e-46 | 155 |
| MS.gene51644.t1 | MTR_2g084405 | 78.750 | 80 | 9 | 1 | 122 | 193 | 1 | 80 | 3.93e-30 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51644.t1 | AT4G20860 | 52.754 | 472 | 203 | 8 | 14 | 469 | 17 | 484 | 3.81e-170 | 489 |
| MS.gene51644.t1 | AT5G44360 | 51.653 | 484 | 212 | 11 | 1 | 469 | 9 | 485 | 1.62e-163 | 473 |
| MS.gene51644.t1 | AT5G44360 | 51.653 | 484 | 212 | 11 | 1 | 469 | 25 | 501 | 2.49e-163 | 473 |
| MS.gene51644.t1 | AT5G44400 | 51.967 | 483 | 208 | 11 | 4 | 465 | 7 | 486 | 5.80e-163 | 471 |
| MS.gene51644.t1 | AT2G34790 | 48.649 | 481 | 229 | 7 | 5 | 469 | 11 | 489 | 1.30e-158 | 460 |
| MS.gene51644.t1 | AT1G30760 | 51.183 | 465 | 206 | 9 | 20 | 467 | 29 | 489 | 1.57e-158 | 460 |
| MS.gene51644.t1 | AT1G30760 | 51.183 | 465 | 206 | 9 | 20 | 467 | 47 | 507 | 2.79e-158 | 460 |
| MS.gene51644.t1 | AT5G44390 | 50.317 | 473 | 215 | 11 | 13 | 468 | 24 | 493 | 8.05e-155 | 451 |
| MS.gene51644.t1 | AT4G20820 | 46.482 | 469 | 231 | 8 | 8 | 466 | 19 | 477 | 4.56e-145 | 426 |
| MS.gene51644.t1 | AT5G44440 | 45.114 | 481 | 243 | 9 | 2 | 466 | 5 | 480 | 9.32e-144 | 422 |
| MS.gene51644.t1 | AT5G44440 | 45.114 | 481 | 243 | 9 | 2 | 466 | 75 | 550 | 1.02e-142 | 422 |
| MS.gene51644.t1 | AT5G44380 | 48.017 | 479 | 227 | 11 | 9 | 468 | 17 | 492 | 6.29e-138 | 407 |
| MS.gene51644.t1 | AT5G44380 | 48.017 | 479 | 227 | 11 | 9 | 468 | 57 | 532 | 1.17e-137 | 408 |
| MS.gene51644.t1 | AT1G26420 | 45.359 | 474 | 236 | 9 | 8 | 465 | 10 | 476 | 5.32e-134 | 397 |
| MS.gene51644.t1 | AT1G26380 | 44.676 | 479 | 244 | 8 | 2 | 465 | 4 | 476 | 1.28e-133 | 396 |
| MS.gene51644.t1 | AT1G26400 | 44.915 | 472 | 245 | 6 | 8 | 467 | 10 | 478 | 1.86e-133 | 396 |
| MS.gene51644.t1 | AT1G26390 | 45.815 | 454 | 228 | 7 | 26 | 465 | 29 | 478 | 1.99e-133 | 396 |
| MS.gene51644.t1 | AT5G44410 | 44.937 | 474 | 239 | 8 | 7 | 466 | 14 | 479 | 2.07e-133 | 396 |
| MS.gene51644.t1 | AT1G26410 | 48.028 | 431 | 205 | 7 | 48 | 465 | 76 | 500 | 8.61e-133 | 395 |
| MS.gene51644.t1 | AT1G30700 | 45.217 | 460 | 228 | 9 | 24 | 467 | 26 | 477 | 4.99e-130 | 387 |
| MS.gene51644.t1 | AT1G30710 | 43.096 | 478 | 253 | 8 | 4 | 467 | 11 | 483 | 2.46e-129 | 385 |
| MS.gene51644.t1 | AT1G34575 | 42.116 | 482 | 244 | 11 | 4 | 465 | 11 | 477 | 3.06e-128 | 382 |
| MS.gene51644.t1 | AT1G11770 | 40.546 | 476 | 267 | 8 | 4 | 467 | 11 | 482 | 1.60e-124 | 373 |
| MS.gene51644.t1 | AT4G20800 | 43.860 | 456 | 236 | 9 | 24 | 465 | 27 | 476 | 2.62e-124 | 372 |
| MS.gene51644.t1 | AT1G01980 | 43.008 | 472 | 245 | 10 | 15 | 467 | 21 | 487 | 7.05e-123 | 369 |
| MS.gene51644.t1 | AT4G20840 | 42.576 | 458 | 244 | 8 | 24 | 467 | 36 | 488 | 1.13e-122 | 369 |
| MS.gene51644.t1 | AT1G30730 | 42.072 | 473 | 259 | 6 | 8 | 467 | 10 | 480 | 2.26e-122 | 367 |
| MS.gene51644.t1 | AT4G20830 | 42.641 | 462 | 248 | 8 | 19 | 467 | 32 | 489 | 1.33e-118 | 358 |
| MS.gene51644.t1 | AT2G34810 | 42.463 | 471 | 250 | 7 | 15 | 467 | 24 | 491 | 1.66e-118 | 358 |
| MS.gene51644.t1 | AT4G20830 | 42.641 | 462 | 248 | 8 | 19 | 467 | 32 | 489 | 4.84e-118 | 358 |
| MS.gene51644.t1 | AT1G30740 | 40.764 | 471 | 262 | 8 | 11 | 467 | 14 | 481 | 3.26e-117 | 354 |
| MS.gene51644.t1 | AT1G30740 | 40.764 | 471 | 262 | 8 | 11 | 467 | 17 | 484 | 3.77e-117 | 354 |
| MS.gene51644.t1 | AT1G30720 | 40.171 | 468 | 265 | 6 | 13 | 467 | 16 | 481 | 4.34e-117 | 354 |
Find 81 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGATTTAGAAGGTATT+TGG | 0.185286 | 3.4:+4791509 | MS.gene51644:CDS |
| GCTTACCTAATAGATGTAAA+TGG | 0.192403 | 3.4:+4790980 | MS.gene51644:CDS |
| TCTTGCATGGAAGATAAATT+TGG | 0.222820 | 3.4:+4791084 | MS.gene51644:CDS |
| TCCATGAACTTTACTTGCTT+TGG | 0.270151 | 3.4:-4790575 | None:intergenic |
| CATCCACTTTATATGCTTGT+TGG | 0.273212 | 3.4:-4791695 | None:intergenic |
| CGCATCAGCATTACTTATAA+TGG | 0.276583 | 3.4:+4791552 | MS.gene51644:CDS |
| CGATGAATCTGCTTGGATTC+AGG | 0.282488 | 3.4:+4790516 | MS.gene51644:CDS |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.4:+4791055 | MS.gene51644:CDS |
| AAAGAGAGTTAAACATTGTT+TGG | 0.291401 | 3.4:-4791267 | None:intergenic |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.4:+4791764 | MS.gene51644:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.4:-4790351 | None:intergenic |
| CTTGATGAATTCAGCCATCT+TGG | 0.311842 | 3.4:-4790301 | None:intergenic |
| AATGAGAGTTTCCTTGAGTT+AGG | 0.320152 | 3.4:+4791325 | MS.gene51644:CDS |
| CACATCAGTGGAGGAGGATT+TGG | 0.323062 | 3.4:+4790911 | MS.gene51644:CDS |
| CTGTTCAGCAAGAGTTCTAT+AGG | 0.327466 | 3.4:-4791424 | None:intergenic |
| TCCAAAGCAAGTAAAGTTCA+TGG | 0.328871 | 3.4:+4790574 | MS.gene51644:CDS |
| TTTCCTTGAGTTAGGGTTGC+AGG | 0.336327 | 3.4:+4791333 | MS.gene51644:CDS |
| CAATACTTGTGGATACAATA+TGG | 0.349912 | 3.4:-4790260 | None:intergenic |
| TCTAAATCAGCTTCTGGTAT+AGG | 0.352720 | 3.4:-4791499 | None:intergenic |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.4:+4790532 | MS.gene51644:CDS |
| ATACCTTCTAAATCAGCTTC+TGG | 0.399342 | 3.4:-4791505 | None:intergenic |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.4:-4791742 | None:intergenic |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.4:+4791765 | MS.gene51644:CDS |
| TAATCAGACTTGGCTATGAA+TGG | 0.426890 | 3.4:-4791466 | None:intergenic |
| CATATTGTATCCACAAGTAT+TGG | 0.430537 | 3.4:+4790261 | MS.gene51644:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.4:-4790464 | None:intergenic |
| CTATGTATTGCCACCTATGA+AGG | 0.438010 | 3.4:-4791174 | None:intergenic |
| CTTACCTAATAGATGTAAAT+GGG | 0.446115 | 3.4:+4790981 | MS.gene51644:CDS |
| ATACCAGAAGCTGATTTAGA+AGG | 0.450654 | 3.4:+4791502 | MS.gene51644:CDS |
| AGAATAGTTGCTCAAAATAG+TGG | 0.457619 | 3.4:+4791226 | MS.gene51644:CDS |
| GAGCAACCAAGCTCCTTCAT+AGG | 0.458493 | 3.4:+4791161 | MS.gene51644:CDS |
| ATGAGAGTTTCCTTGAGTTA+GGG | 0.464989 | 3.4:+4791326 | MS.gene51644:CDS |
| AAGACTGCATTGAAATGAGT+TGG | 0.465070 | 3.4:+4791359 | MS.gene51644:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.4:+4791778 | MS.gene51644:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.4:-4790463 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.4:-4790426 | None:intergenic |
| ATCTTGCCGATGAATCTGCT+TGG | 0.489037 | 3.4:+4790509 | MS.gene51644:CDS |
| TTCAGTGATTCCAATACTTG+TGG | 0.491607 | 3.4:-4790271 | None:intergenic |
| TTCAGAAAAGCTACTACCTA+TGG | 0.493468 | 3.4:-4790203 | None:intergenic |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.4:-4790350 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.4:-4791760 | None:intergenic |
| CTCTTCTACATAATCAGACT+TGG | 0.507678 | 3.4:-4791476 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.509234 | 3.4:+4791692 | MS.gene51644:CDS |
| AATCAGACTTGGCTATGAAT+GGG | 0.513621 | 3.4:-4791465 | None:intergenic |
| ATTCTTGATAGAAAAGCAAT+GGG | 0.518994 | 3.4:+4791007 | MS.gene51644:CDS |
| GAGGATTTGGTACCTTGGTA+AGG | 0.527609 | 3.4:+4790924 | MS.gene51644:CDS |
| GTTCAACATTCAAAGAACAT+TGG | 0.536377 | 3.4:+4791132 | MS.gene51644:CDS |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.4:+4790586 | MS.gene51644:CDS |
| TGTTCAGCAAGAGTTCTATA+GGG | 0.539613 | 3.4:-4791423 | None:intergenic |
| ATGCAGTGCATGTTAACCAT+AGG | 0.544066 | 3.4:+4790187 | MS.gene51644:CDS |
| CACTGAAACAAAATCCAAGA+TGG | 0.547598 | 3.4:+4790287 | MS.gene51644:CDS |
| AATTCTTGATAGAAAAGCAA+TGG | 0.550485 | 3.4:+4791006 | MS.gene51644:CDS |
| TGGAGGAGGATTTGGTACCT+TGG | 0.552904 | 3.4:+4790919 | MS.gene51644:CDS |
| TTTCCTGCAACCCTAACTCA+AGG | 0.554409 | 3.4:-4791336 | None:intergenic |
| ACATACAATACTTTGTCAAG+TGG | 0.554747 | 3.4:+4791650 | MS.gene51644:CDS |
| ATGTTGAACACTGTAACAAT+AGG | 0.557897 | 3.4:-4791118 | None:intergenic |
| TTACTTATAATGGAGCCATA+TGG | 0.558832 | 3.4:+4791562 | MS.gene51644:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.4:-4790346 | None:intergenic |
| GGTGGTCACATCAGTGGAGG+AGG | 0.567357 | 3.4:+4790905 | MS.gene51644:intron |
| CTTATAATGGAGCCATATGG+TGG | 0.568422 | 3.4:+4791565 | MS.gene51644:CDS |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.4:+4790400 | MS.gene51644:CDS |
| TTGAACACTGTAACAATAGG+TGG | 0.572928 | 3.4:-4791115 | None:intergenic |
| CTGAATCCAAGCAGATTCAT+CGG | 0.574804 | 3.4:-4790515 | None:intergenic |
| GAATCTGCTTGGATTCAGGC+TGG | 0.577913 | 3.4:+4790520 | MS.gene51644:CDS |
| TTGCCACCTATGAAGGAGCT+TGG | 0.579989 | 3.4:-4791167 | None:intergenic |
| GGTACCTTGGTAAGGAAACA+TGG | 0.586233 | 3.4:+4790932 | MS.gene51644:CDS |
| CATACAATACTTTGTCAAGT+GGG | 0.588214 | 3.4:+4791651 | MS.gene51644:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.4:+4790418 | MS.gene51644:CDS |
| AAACTAATTAATTACCTGCA+GGG | 0.596070 | 3.4:-4790600 | None:intergenic |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.4:+4790393 | MS.gene51644:CDS |
| GGAGTAGGTGGTCACATCAG+TGG | 0.610281 | 3.4:+4790899 | MS.gene51644:intron |
| CAAGGGAGGGAATTCAGAAG+AGG | 0.618945 | 3.4:-4791940 | None:intergenic |
| CAAGATTAATGTCGATCGAA+CGG | 0.627262 | 3.4:-4790492 | None:intergenic |
| CAACCAAGCTCCTTCATAGG+TGG | 0.630946 | 3.4:+4791164 | MS.gene51644:CDS |
| AGAACTCTTGCTGAACAGAA+CGG | 0.634545 | 3.4:+4791429 | MS.gene51644:CDS |
| GAAGGTGTCATAATGAGAAG+AGG | 0.648177 | 3.4:-4790328 | None:intergenic |
| TTATAATGGAGCCATATGGT+GGG | 0.651843 | 3.4:+4791566 | MS.gene51644:CDS |
| AAGACCATGTTTCCTTACCA+AGG | 0.654647 | 3.4:-4790936 | None:intergenic |
| GTAGGTGGTCACATCAGTGG+AGG | 0.667986 | 3.4:+4790902 | MS.gene51644:intron |
| ATTCAAAGAACATTGGAACA+AGG | 0.678567 | 3.4:+4791139 | MS.gene51644:CDS |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.4:+4790403 | MS.gene51644:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTAATTAATTACCTGCA+GGG | - | chr3.4:4790603-4790622 | None:intergenic | 25.0% |
| ! | AAAGAGAGTTAAACATTGTT+TGG | - | chr3.4:4791270-4791289 | None:intergenic | 25.0% |
| ! | AATTCTTGATAGAAAAGCAA+TGG | + | chr3.4:4791006-4791025 | MS.gene51644:CDS | 25.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.4:4790466-4790485 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.4:4790860-4790879 | MS.gene51644:intron | 25.0% |
| ! | ATTCTTGATAGAAAAGCAAT+GGG | + | chr3.4:4791007-4791026 | MS.gene51644:CDS | 25.0% |
| ! | CTTACCTAATAGATGTAAAT+GGG | + | chr3.4:4790981-4791000 | MS.gene51644:CDS | 25.0% |
| !! | TTTTCCCATTTACATCTATT+AGG | - | chr3.4:4790988-4791007 | None:intergenic | 25.0% |
| !!! | CAATCAGTTTTATATATTGC+AGG | + | chr3.4:4791385-4791404 | MS.gene51644:CDS | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.4:4791778-4791797 | MS.gene51644:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.4:4790429-4790448 | None:intergenic | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.4:4791692-4791711 | MS.gene51644:CDS | 30.0% | |
| ACATACAATACTTTGTCAAG+TGG | + | chr3.4:4791650-4791669 | MS.gene51644:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.4:4791226-4791245 | MS.gene51644:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.4:4791765-4791784 | MS.gene51644:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.4:4790376-4790395 | MS.gene51644:CDS | 30.0% | |
| ATGTTGAACACTGTAACAAT+AGG | - | chr3.4:4791121-4791140 | None:intergenic | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.4:4791139-4791158 | MS.gene51644:CDS | 30.0% | |
| CAAACTAATTAATTACCTGC+AGG | - | chr3.4:4790604-4790623 | None:intergenic | 30.0% | |
| CAATACTTGTGGATACAATA+TGG | - | chr3.4:4790263-4790282 | None:intergenic | 30.0% | |
| CATACAATACTTTGTCAAGT+GGG | + | chr3.4:4791651-4791670 | MS.gene51644:CDS | 30.0% | |
| CATATTGTATCCACAAGTAT+TGG | + | chr3.4:4790261-4790280 | MS.gene51644:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.4:4790467-4790486 | None:intergenic | 30.0% | |
| GCTTACCTAATAGATGTAAA+TGG | + | chr3.4:4790980-4790999 | MS.gene51644:CDS | 30.0% | |
| GTTCAACATTCAAAGAACAT+TGG | + | chr3.4:4791132-4791151 | MS.gene51644:CDS | 30.0% | |
| TCTTGCATGGAAGATAAATT+TGG | + | chr3.4:4791084-4791103 | MS.gene51644:CDS | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.4:4790353-4790372 | None:intergenic | 30.0% | |
| ! | AACAAGTTTCCTTTTCTATG+TGG | - | chr3.4:4791628-4791647 | None:intergenic | 30.0% |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.4:4790377-4790396 | None:intergenic | 30.0% |
| ! | ATGTTTAACTCTCTTTTCCT+TGG | + | chr3.4:4791274-4791293 | MS.gene51644:CDS | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.4:4791911-4791930 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.4:4791910-4791929 | None:intergenic | 30.0% |
| ! | TTAAGTTTTCTTTTCCTCCA+AGG | - | chr3.4:4791294-4791313 | None:intergenic | 30.0% |
| ! | TTACTTATAATGGAGCCATA+TGG | + | chr3.4:4791562-4791581 | MS.gene51644:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.4:4791621-4791640 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.4:4791622-4791641 | None:intergenic | 30.0% |
| !! | AAGCTGATTTAGAAGGTATT+TGG | + | chr3.4:4791509-4791528 | MS.gene51644:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.4:4791024-4791043 | MS.gene51644:CDS | 30.0% |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.4:4791359-4791378 | MS.gene51644:CDS | 35.0% | |
| AATCAGACTTGGCTATGAAT+GGG | - | chr3.4:4791468-4791487 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCTTGAGTT+AGG | + | chr3.4:4791325-4791344 | MS.gene51644:CDS | 35.0% | |
| ATACCTTCTAAATCAGCTTC+TGG | - | chr3.4:4791508-4791527 | None:intergenic | 35.0% | |
| ATGAGAGTTTCCTTGAGTTA+GGG | + | chr3.4:4791326-4791345 | MS.gene51644:CDS | 35.0% | |
| CAAGATTAATGTCGATCGAA+CGG | - | chr3.4:4790495-4790514 | None:intergenic | 35.0% | |
| CACTGAAACAAAATCCAAGA+TGG | + | chr3.4:4790287-4790306 | MS.gene51644:CDS | 35.0% | |
| CATCCACTTTATATGCTTGT+TGG | - | chr3.4:4791698-4791717 | None:intergenic | 35.0% | |
| CGCATCAGCATTACTTATAA+TGG | + | chr3.4:4791552-4791571 | MS.gene51644:CDS | 35.0% | |
| CTCTTCTACATAATCAGACT+TGG | - | chr3.4:4791479-4791498 | None:intergenic | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.4:4790354-4790373 | None:intergenic | 35.0% | |
| CTTGTATCTATAAGCTCTCA+AGG | + | chr3.4:4790731-4790750 | MS.gene51644:intron | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.4:4791764-4791783 | MS.gene51644:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGTT+TGG | + | chr3.4:4791861-4791880 | MS.gene51644:intron | 35.0% | |
| TAATCAGACTTGGCTATGAA+TGG | - | chr3.4:4791469-4791488 | None:intergenic | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.4:4791763-4791782 | None:intergenic | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.4:4790574-4790593 | MS.gene51644:CDS | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.4:4790578-4790597 | None:intergenic | 35.0% | |
| TCTAAATCAGCTTCTGGTAT+AGG | - | chr3.4:4791502-4791521 | None:intergenic | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.4:4790349-4790368 | None:intergenic | 35.0% | |
| TGTTCAGCAAGAGTTCTATA+GGG | - | chr3.4:4791426-4791445 | None:intergenic | 35.0% | |
| TTCAGAAAAGCTACTACCTA+TGG | - | chr3.4:4790206-4790225 | None:intergenic | 35.0% | |
| TTCAGTGATTCCAATACTTG+TGG | - | chr3.4:4790274-4790293 | None:intergenic | 35.0% | |
| TTGAACACTGTAACAATAGG+TGG | - | chr3.4:4791118-4791137 | None:intergenic | 35.0% | |
| ! | ATACCAGAAGCTGATTTAGA+AGG | + | chr3.4:4791502-4791521 | MS.gene51644:CDS | 35.0% |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.4:4791616-4791635 | MS.gene51644:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.4:4791912-4791931 | None:intergenic | 35.0% |
| ! | TTATAATGGAGCCATATGGT+GGG | + | chr3.4:4791566-4791585 | MS.gene51644:CDS | 35.0% |
| ! | TTTAACTCTCTTTTCCTTGG+AGG | + | chr3.4:4791277-4791296 | MS.gene51644:CDS | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.4:4791745-4791764 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.4:4791902-4791921 | MS.gene51644:intron | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.4:4791023-4791042 | MS.gene51644:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.4:4791034-4791053 | MS.gene51644:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.4:4791071-4791090 | MS.gene51644:CDS | 35.0% |
| AAGACCATGTTTCCTTACCA+AGG | - | chr3.4:4790939-4790958 | None:intergenic | 40.0% | |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.4:4790878-4790897 | MS.gene51644:intron | 40.0% | |
| AGAACTCTTGCTGAACAGAA+CGG | + | chr3.4:4791429-4791448 | MS.gene51644:CDS | 40.0% | |
| ATGCAGTGCATGTTAACCAT+AGG | + | chr3.4:4790187-4790206 | MS.gene51644:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.4:4790877-4790896 | None:intergenic | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.4:4791825-4791844 | MS.gene51644:intron | 40.0% | |
| CTATGTATTGCCACCTATGA+AGG | - | chr3.4:4791177-4791196 | None:intergenic | 40.0% | |
| CTGAATCCAAGCAGATTCAT+CGG | - | chr3.4:4790518-4790537 | None:intergenic | 40.0% | |
| CTGTTCAGCAAGAGTTCTAT+AGG | - | chr3.4:4791427-4791446 | None:intergenic | 40.0% | |
| CTTGATGAATTCAGCCATCT+TGG | - | chr3.4:4790304-4790323 | None:intergenic | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.4:4791826-4791845 | MS.gene51644:intron | 40.0% | |
| ! | CTTATAATGGAGCCATATGG+TGG | + | chr3.4:4791565-4791584 | MS.gene51644:CDS | 40.0% |
| ! | TCACTCATTTTCCCACCATA+TGG | - | chr3.4:4791580-4791599 | None:intergenic | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.4:4791844-4791863 | MS.gene51644:intron | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.4:4791843-4791862 | MS.gene51644:intron | 40.0% |
| !! | ATATGTCCAAGTGTTGGAGT+AGG | + | chr3.4:4790884-4790903 | MS.gene51644:intron | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.4:4790331-4790350 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.4:4791037-4791056 | MS.gene51644:CDS | 40.0% |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.4:4790586-4790605 | MS.gene51644:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.4:4790418-4790437 | MS.gene51644:CDS | 45.0% | |
| ATCTTGCCGATGAATCTGCT+TGG | + | chr3.4:4790509-4790528 | MS.gene51644:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.4:4790532-4790551 | MS.gene51644:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.4:4790403-4790422 | MS.gene51644:CDS | 45.0% | |
| CGATGAATCTGCTTGGATTC+AGG | + | chr3.4:4790516-4790535 | MS.gene51644:CDS | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.4:4790393-4790412 | MS.gene51644:CDS | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.4:4791824-4791843 | MS.gene51644:intron | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.4:4790932-4790951 | MS.gene51644:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.4:4790400-4790419 | MS.gene51644:CDS | 45.0% | |
| TTTCCTGCAACCCTAACTCA+AGG | - | chr3.4:4791339-4791358 | None:intergenic | 45.0% | |
| TTTCCTTGAGTTAGGGTTGC+AGG | + | chr3.4:4791333-4791352 | MS.gene51644:CDS | 45.0% | |
| ! | GAGGATTTGGTACCTTGGTA+AGG | + | chr3.4:4790924-4790943 | MS.gene51644:CDS | 45.0% |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.4:4791055-4791074 | MS.gene51644:CDS | 45.0% |
| !! | GGCTATGAATGGGCTTTTGT+AGG | - | chr3.4:4791458-4791477 | None:intergenic | 45.0% |
| CAACCAAGCTCCTTCATAGG+TGG | + | chr3.4:4791164-4791183 | MS.gene51644:CDS | 50.0% | |
| CACATCAGTGGAGGAGGATT+TGG | + | chr3.4:4790911-4790930 | MS.gene51644:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.4:4790520-4790539 | MS.gene51644:CDS | 50.0% | |
| GAGCAACCAAGCTCCTTCAT+AGG | + | chr3.4:4791161-4791180 | MS.gene51644:CDS | 50.0% | |
| TGACCACCTACTCCAACACT+TGG | - | chr3.4:4790893-4790912 | None:intergenic | 50.0% | |
| TTGCCACCTATGAAGGAGCT+TGG | - | chr3.4:4791170-4791189 | None:intergenic | 50.0% | |
| ! | TGGAGGAGGATTTGGTACCT+TGG | + | chr3.4:4790919-4790938 | MS.gene51644:CDS | 50.0% |
| !! | TGTCCAAGTGTTGGAGTAGG+TGG | + | chr3.4:4790887-4790906 | MS.gene51644:intron | 50.0% |
| GGAGTAGGTGGTCACATCAG+TGG | + | chr3.4:4790899-4790918 | MS.gene51644:intron | 55.0% | |
| GTAGGTGGTCACATCAGTGG+AGG | + | chr3.4:4790902-4790921 | MS.gene51644:intron | 55.0% | |
| GGTGGTCACATCAGTGGAGG+AGG | + | chr3.4:4790905-4790924 | MS.gene51644:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 4790109 | 4791949 | 4790109 | ID=MS.gene51644 |
| chr3.4 | mRNA | 4790109 | 4791949 | 4790109 | ID=MS.gene51644.t1;Parent=MS.gene51644 |
| chr3.4 | exon | 4790109 | 4790607 | 4790109 | ID=MS.gene51644.t1.exon1;Parent=MS.gene51644.t1 |
| chr3.4 | CDS | 4790109 | 4790607 | 4790109 | ID=cds.MS.gene51644.t1;Parent=MS.gene51644.t1 |
| chr3.4 | exon | 4790906 | 4791799 | 4790906 | ID=MS.gene51644.t1.exon2;Parent=MS.gene51644.t1 |
| chr3.4 | CDS | 4790906 | 4791799 | 4790906 | ID=cds.MS.gene51644.t1;Parent=MS.gene51644.t1 |
| chr3.4 | exon | 4791924 | 4791949 | 4791924 | ID=MS.gene51644.t1.exon3;Parent=MS.gene51644.t1 |
| chr3.4 | CDS | 4791924 | 4791949 | 4791924 | ID=cds.MS.gene51644.t1;Parent=MS.gene51644.t1 |
| Gene Sequence |
| Protein sequence |
