Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51646.t1 | AES68535.2 | 89.7 | 505 | 26 | 1 | 1 | 479 | 27 | 531 | 1.10E-258 | 902.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51646.t1 | Q9SUC6 | 53.4 | 485 | 193 | 6 | 15 | 468 | 47 | 529 | 2.3e-148 | 526.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51646.t1 | G7J0D0 | 89.7 | 505 | 26 | 1 | 1 | 479 | 27 | 531 | 7.9e-259 | 902.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28726 | MS.gene51646 | PPI |
| MS.gene028369 | MS.gene51646 | PPI |
| MS.gene35083 | MS.gene51646 | PPI |
| MS.gene28717 | MS.gene51646 | PPI |
| MS.gene51646 | MS.gene028354 | PPI |
| MS.gene28723 | MS.gene51646 | PPI |
| MS.gene028352 | MS.gene51646 | PPI |
| MS.gene35102 | MS.gene51646 | PPI |
| MS.gene51646 | MS.gene35103 | PPI |
| MS.gene51646 | MS.gene36437 | PPI |
| MS.gene28742 | MS.gene51646 | PPI |
| MS.gene36445 | MS.gene51646 | PPI |
| MS.gene28725 | MS.gene51646 | PPI |
| MS.gene36439 | MS.gene51646 | PPI |
| MS.gene51646 | MS.gene028346 | PPI |
| MS.gene35104 | MS.gene51646 | PPI |
| MS.gene51646 | MS.gene36435 | PPI |
| MS.gene36438 | MS.gene51646 | PPI |
| MS.gene28724 | MS.gene51646 | PPI |
| MS.gene028347 | MS.gene51646 | PPI |
| MS.gene35106 | MS.gene51646 | PPI |
| MS.gene02146 | MS.gene51646 | PPI |
| MS.gene28727 | MS.gene51646 | PPI |
| MS.gene35105 | MS.gene51646 | PPI |
| MS.gene003357 | MS.gene51646 | PPI |
| MS.gene36421 | MS.gene51646 | PPI |
| MS.gene51646 | MS.gene028353 | PPI |
| MS.gene51646 | MS.gene047977 | PPI |
| MS.gene51646 | MS.gene36444 | PPI |
| MS.gene028357 | MS.gene51646 | PPI |
| MS.gene35114 | MS.gene51646 | PPI |
| MS.gene35113 | MS.gene51646 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51646.t1 | MTR_3g009880 | 89.307 | 505 | 28 | 2 | 1 | 479 | 27 | 531 | 0.0 | 908 |
| MS.gene51646.t1 | MTR_3g009760 | 81.891 | 497 | 62 | 3 | 1 | 469 | 27 | 523 | 0.0 | 831 |
| MS.gene51646.t1 | MTR_3g009820 | 80.885 | 497 | 67 | 3 | 1 | 469 | 27 | 523 | 0.0 | 825 |
| MS.gene51646.t1 | MTR_4g094488 | 52.684 | 503 | 200 | 7 | 1 | 466 | 34 | 535 | 0.0 | 517 |
| MS.gene51646.t1 | MTR_2g031420 | 52.800 | 500 | 200 | 8 | 1 | 469 | 34 | 528 | 3.10e-179 | 513 |
| MS.gene51646.t1 | MTR_2g031460 | 52.218 | 496 | 201 | 8 | 1 | 465 | 34 | 524 | 6.76e-178 | 510 |
| MS.gene51646.t1 | MTR_4g094495 | 52.390 | 502 | 203 | 7 | 1 | 466 | 37 | 538 | 1.64e-176 | 506 |
| MS.gene51646.t1 | MTR_4g094478 | 51.394 | 502 | 210 | 6 | 1 | 468 | 34 | 535 | 3.47e-176 | 506 |
| MS.gene51646.t1 | MTR_4g094492 | 51.690 | 503 | 208 | 7 | 1 | 468 | 34 | 536 | 4.40e-176 | 505 |
| MS.gene51646.t1 | MTR_2g031390 | 49.307 | 505 | 221 | 6 | 1 | 472 | 31 | 533 | 1.28e-175 | 504 |
| MS.gene51646.t1 | MTR_4g094518 | 50.298 | 503 | 215 | 6 | 1 | 470 | 32 | 532 | 3.20e-168 | 485 |
| MS.gene51646.t1 | MTR_2g031560 | 47.732 | 507 | 223 | 8 | 1 | 471 | 26 | 526 | 8.44e-168 | 485 |
| MS.gene51646.t1 | MTR_4g094418 | 54.358 | 436 | 163 | 5 | 70 | 469 | 1 | 436 | 2.31e-167 | 486 |
| MS.gene51646.t1 | MTR_4g094480 | 48.000 | 500 | 226 | 6 | 1 | 466 | 34 | 533 | 6.37e-166 | 479 |
| MS.gene51646.t1 | MTR_4g094520 | 50.413 | 484 | 205 | 9 | 19 | 470 | 54 | 534 | 6.11e-165 | 477 |
| MS.gene51646.t1 | MTR_4g094498 | 47.485 | 497 | 228 | 6 | 2 | 468 | 41 | 534 | 4.46e-163 | 473 |
| MS.gene51646.t1 | MTR_2g031590 | 47.629 | 485 | 212 | 8 | 22 | 468 | 52 | 532 | 2.83e-162 | 470 |
| MS.gene51646.t1 | MTR_2g031490 | 43.825 | 502 | 246 | 7 | 1 | 468 | 39 | 538 | 8.61e-147 | 431 |
| MS.gene51646.t1 | MTR_2g031510 | 43.936 | 503 | 244 | 8 | 1 | 468 | 41 | 540 | 2.31e-146 | 429 |
| MS.gene51646.t1 | MTR_4g091200 | 45.902 | 488 | 231 | 7 | 11 | 466 | 38 | 524 | 3.59e-146 | 429 |
| MS.gene51646.t1 | MTR_3g009850 | 88.889 | 225 | 25 | 0 | 245 | 469 | 5 | 229 | 4.19e-146 | 417 |
| MS.gene51646.t1 | MTR_4g088355 | 47.807 | 456 | 206 | 6 | 42 | 466 | 77 | 531 | 5.10e-144 | 423 |
| MS.gene51646.t1 | MTR_6g017205 | 44.376 | 489 | 239 | 7 | 10 | 466 | 37 | 524 | 1.39e-141 | 417 |
| MS.gene51646.t1 | MTR_4g057990 | 44.820 | 473 | 232 | 5 | 22 | 466 | 49 | 520 | 1.86e-141 | 416 |
| MS.gene51646.t1 | MTR_4g091150 | 45.238 | 504 | 237 | 12 | 1 | 468 | 40 | 540 | 1.26e-139 | 412 |
| MS.gene51646.t1 | MTR_4g091190 | 46.272 | 456 | 211 | 7 | 42 | 466 | 74 | 526 | 9.98e-139 | 410 |
| MS.gene51646.t1 | MTR_4g094515 | 43.400 | 500 | 249 | 7 | 1 | 468 | 653 | 1150 | 1.53e-137 | 424 |
| MS.gene51646.t1 | MTR_4g094515 | 42.000 | 500 | 254 | 7 | 1 | 466 | 41 | 538 | 2.67e-130 | 405 |
| MS.gene51646.t1 | MTR_4g091170 | 45.133 | 452 | 200 | 6 | 45 | 466 | 107 | 540 | 2.77e-134 | 400 |
| MS.gene51646.t1 | MTR_2g023590 | 43.764 | 457 | 221 | 8 | 42 | 466 | 12 | 464 | 1.72e-129 | 384 |
| MS.gene51646.t1 | MTR_1g076830 | 39.451 | 474 | 252 | 8 | 26 | 465 | 60 | 532 | 1.73e-113 | 345 |
| MS.gene51646.t1 | MTR_2g031600 | 55.696 | 158 | 67 | 2 | 312 | 468 | 245 | 400 | 3.44e-56 | 192 |
| MS.gene51646.t1 | MTR_2g031600 | 43.750 | 192 | 81 | 3 | 22 | 186 | 53 | 244 | 1.65e-48 | 172 |
| MS.gene51646.t1 | MTR_2g084370 | 88.764 | 89 | 6 | 1 | 94 | 178 | 1 | 89 | 1.83e-49 | 164 |
| MS.gene51646.t1 | MTR_2g084405 | 60.000 | 80 | 6 | 2 | 94 | 147 | 1 | 80 | 1.86e-17 | 78.2 |
| MS.gene51646.t1 | MTR_8g062280 | 53.846 | 52 | 24 | 0 | 414 | 465 | 21 | 72 | 5.13e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51646.t1 | AT4G20860 | 53.846 | 455 | 180 | 6 | 42 | 468 | 77 | 529 | 2.34e-179 | 513 |
| MS.gene51646.t1 | AT5G44360 | 50.992 | 504 | 208 | 11 | 1 | 470 | 34 | 532 | 5.25e-173 | 497 |
| MS.gene51646.t1 | AT5G44360 | 50.992 | 504 | 208 | 11 | 1 | 470 | 50 | 548 | 7.21e-173 | 497 |
| MS.gene51646.t1 | AT5G44400 | 52.083 | 480 | 191 | 10 | 22 | 466 | 59 | 534 | 2.12e-166 | 481 |
| MS.gene51646.t1 | AT1G30760 | 49.585 | 482 | 204 | 8 | 18 | 466 | 55 | 530 | 1.75e-163 | 473 |
| MS.gene51646.t1 | AT1G30760 | 49.585 | 482 | 204 | 8 | 18 | 466 | 73 | 548 | 2.09e-163 | 473 |
| MS.gene51646.t1 | AT2G34790 | 49.163 | 478 | 204 | 7 | 22 | 466 | 57 | 528 | 3.61e-161 | 467 |
| MS.gene51646.t1 | AT5G44390 | 49.795 | 488 | 200 | 11 | 22 | 470 | 61 | 542 | 8.62e-157 | 456 |
| MS.gene51646.t1 | AT4G20820 | 44.600 | 500 | 240 | 8 | 1 | 471 | 38 | 529 | 8.59e-151 | 441 |
| MS.gene51646.t1 | AT5G44440 | 43.762 | 505 | 248 | 9 | 1 | 472 | 32 | 533 | 5.94e-150 | 438 |
| MS.gene51646.t1 | AT5G44440 | 43.762 | 505 | 248 | 9 | 1 | 472 | 102 | 603 | 7.57e-149 | 438 |
| MS.gene51646.t1 | AT1G26420 | 44.554 | 505 | 240 | 10 | 1 | 471 | 30 | 528 | 5.57e-148 | 433 |
| MS.gene51646.t1 | AT5G44380 | 48.081 | 495 | 222 | 10 | 5 | 466 | 45 | 537 | 3.70e-146 | 429 |
| MS.gene51646.t1 | AT1G26390 | 45.021 | 482 | 230 | 8 | 22 | 471 | 52 | 530 | 1.06e-145 | 427 |
| MS.gene51646.t1 | AT5G44380 | 48.081 | 495 | 222 | 10 | 5 | 466 | 85 | 577 | 1.23e-145 | 429 |
| MS.gene51646.t1 | AT1G26380 | 46.138 | 479 | 221 | 8 | 22 | 468 | 52 | 525 | 2.32e-145 | 427 |
| MS.gene51646.t1 | AT1G26410 | 44.008 | 484 | 236 | 8 | 18 | 470 | 72 | 551 | 1.16e-142 | 421 |
| MS.gene51646.t1 | AT5G44410 | 45.417 | 480 | 220 | 10 | 26 | 471 | 61 | 532 | 7.28e-141 | 415 |
| MS.gene51646.t1 | AT1G30710 | 42.801 | 507 | 240 | 11 | 2 | 469 | 34 | 529 | 1.61e-140 | 414 |
| MS.gene51646.t1 | AT1G34575 | 42.656 | 497 | 247 | 9 | 2 | 467 | 34 | 523 | 7.88e-140 | 412 |
| MS.gene51646.t1 | AT1G30700 | 46.555 | 479 | 214 | 10 | 26 | 468 | 54 | 526 | 1.60e-137 | 406 |
| MS.gene51646.t1 | AT1G26400 | 44.118 | 476 | 232 | 8 | 20 | 465 | 50 | 521 | 3.83e-136 | 403 |
| MS.gene51646.t1 | AT4G20840 | 40.519 | 501 | 261 | 9 | 1 | 468 | 39 | 535 | 8.66e-135 | 400 |
| MS.gene51646.t1 | AT4G20800 | 42.052 | 497 | 254 | 8 | 1 | 466 | 30 | 523 | 3.57e-133 | 395 |
| MS.gene51646.t1 | AT1G01980 | 41.667 | 504 | 256 | 9 | 1 | 468 | 33 | 534 | 7.74e-133 | 395 |
| MS.gene51646.t1 | AT1G11770 | 41.283 | 499 | 260 | 8 | 1 | 468 | 33 | 529 | 5.78e-132 | 392 |
| MS.gene51646.t1 | AT1G30730 | 43.202 | 456 | 226 | 7 | 42 | 467 | 72 | 524 | 2.01e-129 | 386 |
| MS.gene51646.t1 | AT4G20830 | 41.000 | 500 | 260 | 9 | 1 | 468 | 40 | 536 | 8.74e-129 | 385 |
| MS.gene51646.t1 | AT4G20830 | 41.000 | 500 | 260 | 9 | 1 | 468 | 40 | 536 | 2.20e-128 | 384 |
| MS.gene51646.t1 | AT1G30720 | 40.281 | 499 | 263 | 10 | 1 | 467 | 30 | 525 | 2.43e-125 | 375 |
| MS.gene51646.t1 | AT2G34810 | 39.406 | 505 | 268 | 10 | 1 | 468 | 36 | 539 | 4.68e-123 | 370 |
| MS.gene51646.t1 | AT1G30740 | 40.120 | 501 | 265 | 8 | 1 | 468 | 30 | 528 | 1.15e-117 | 356 |
| MS.gene51646.t1 | AT1G30740 | 40.120 | 501 | 265 | 8 | 1 | 468 | 33 | 531 | 1.42e-117 | 356 |
Find 90 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGCATCAAGTACTTTA+AGG | 0.182488 | 3.4:+4813526 | MS.gene51646:CDS |
| AAGCTGGTTTAGAAGGTATT+TGG | 0.185112 | 3.4:+4813192 | MS.gene51646:CDS |
| AATGAGAGTTTCCCTGAATT+AGG | 0.214946 | 3.4:+4813008 | MS.gene51646:CDS |
| CACATTAGCATTACTTATAA+TGG | 0.248787 | 3.4:+4813235 | MS.gene51646:CDS |
| TTGTCTTGCAACCCTAATTC+AGG | 0.255332 | 3.4:-4813020 | None:intergenic |
| GTTCACCATTCATAAAATTC+TGG | 0.274997 | 3.4:+4812815 | MS.gene51646:CDS |
| GGAGGTAGTGCTGCTAGTTT+TGG | 0.287867 | 3.4:+4812738 | MS.gene51646:CDS |
| CATCCACTTTATATGTTTGT+TGG | 0.301040 | 3.4:-4813378 | None:intergenic |
| TCCTTCCATTTACATCTATA+AGG | 0.320941 | 3.4:-4812668 | None:intergenic |
| CTTGATGAGTTCACCCATCT+TGG | 0.322731 | 3.4:-4812012 | None:intergenic |
| GTGGATTTGGTACCTTGTTT+AGG | 0.323328 | 3.4:+4812607 | MS.gene51646:CDS |
| TAGAGGTGGTGGAATTAATT+AGG | 0.323700 | 3.4:+4813652 | MS.gene51646:CDS |
| GGGCTGCATATTACAACTAT+AGG | 0.325111 | 3.4:+4813447 | MS.gene51646:CDS |
| GCTTACCTTATAGATGTAAA+TGG | 0.326848 | 3.4:+4812663 | MS.gene51646:CDS |
| AATCTCTTTGCTGCATAGTA+TGG | 0.329557 | 3.4:-4812085 | None:intergenic |
| CACATCAGTGGAGGTGGATT+TGG | 0.356454 | 3.4:+4812594 | MS.gene51646:CDS |
| ATGAGAGTTTCCCTGAATTA+GGG | 0.357011 | 3.4:+4813009 | MS.gene51646:CDS |
| CTGTTAAGCAAGAGTTCTAT+AGG | 0.363323 | 3.4:-4813107 | None:intergenic |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.4:-4813182 | None:intergenic |
| GCTACTCAGAAGCAAGTGTT+TGG | 0.368108 | 3.4:+4813507 | MS.gene51646:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.4:+4812243 | MS.gene51646:CDS |
| AGCAGCACTACCTCCTCTTA+TGG | 0.383413 | 3.4:-4812730 | None:intergenic |
| AAATGAATCTGCTTGGATTC+AGG | 0.391403 | 3.4:+4812227 | MS.gene51646:CDS |
| TAGTCAGATTTGGCTTTGAA+TGG | 0.394226 | 3.4:-4813149 | None:intergenic |
| TTCAGAATAGCTACTAACTA+TGG | 0.395170 | 3.4:-4811920 | None:intergenic |
| TCACTCATTCTTCCACCATA+TGG | 0.398978 | 3.4:-4813260 | None:intergenic |
| AGAATAGTTGCTCAAACTAA+TGG | 0.401600 | 3.4:+4812909 | MS.gene51646:CDS |
| TGGGGCATCAAGTACTTTAA+GGG | 0.402731 | 3.4:+4813527 | MS.gene51646:CDS |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.4:-4813188 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.4:+4813185 | MS.gene51646:CDS |
| CTACTCAGAAGCAAGTGTTT+GGG | 0.418460 | 3.4:+4813508 | MS.gene51646:CDS |
| GGCTGCATATTACAACTATA+GGG | 0.426482 | 3.4:+4813448 | MS.gene51646:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.427868 | 3.4:-4813425 | None:intergenic |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.4:-4812175 | None:intergenic |
| ATGTTTAATTCTCTCTTCCT+TGG | 0.438442 | 3.4:+4812957 | MS.gene51646:CDS |
| CCACAAAACTCATTCATAGA+TGG | 0.453753 | 3.4:+4812847 | MS.gene51646:CDS |
| TCATTCTTGCATGGAAGATA+AGG | 0.459271 | 3.4:+4812763 | MS.gene51646:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.467290 | 3.4:+4813461 | MS.gene51646:CDS |
| GATTCTTAATAGAAAATCAA+TGG | 0.470027 | 3.4:+4812689 | MS.gene51646:CDS |
| TAAGGGAAACTTTAAGCGAT+TGG | 0.470910 | 3.4:+4813544 | MS.gene51646:CDS |
| TGTCTTGCAACCCTAATTCA+GGG | 0.475992 | 3.4:-4813019 | None:intergenic |
| AAGACTGCATTGAAATGAGT+TGG | 0.478707 | 3.4:+4813042 | MS.gene51646:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.4:-4812174 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.4:-4812137 | None:intergenic |
| AGTCAGATTTGGCTTTGAAT+GGG | 0.479319 | 3.4:-4813148 | None:intergenic |
| CATTGAAACAAAATCCAAGA+TGG | 0.486669 | 3.4:+4811998 | MS.gene51646:CDS |
| AGTCCAACAAACATATAAAG+TGG | 0.489218 | 3.4:+4813375 | MS.gene51646:CDS |
| ATATTGCAAATGAATCTGCT+TGG | 0.491824 | 3.4:+4812220 | MS.gene51646:CDS |
| ATTCATAAAATTCTGGAAGA+AGG | 0.493097 | 3.4:+4812822 | MS.gene51646:CDS |
| TTCAATGATTCCAACACTTG+TGG | 0.495973 | 3.4:-4811982 | None:intergenic |
| ATACTATGCAGCAAAGAGAT+TGG | 0.503786 | 3.4:+4812087 | MS.gene51646:CDS |
| CTTGTACAACATACAATACA+TGG | 0.505452 | 3.4:+4813325 | MS.gene51646:CDS |
| TTCCAACACTTGTGGATACA+AGG | 0.515360 | 3.4:-4811974 | None:intergenic |
| GGCGGTCACATCAGTGGAGG+TGG | 0.518000 | 3.4:+4812588 | MS.gene51646:intron |
| ATTCTTAATAGAAAATCAAT+GGG | 0.522100 | 3.4:+4812690 | MS.gene51646:CDS |
| ATCCTTGTATCCACAAGTGT+TGG | 0.532948 | 3.4:+4811972 | MS.gene51646:CDS |
| GGTACCTTGTTTAGGAAACA+TGG | 0.537180 | 3.4:+4812615 | MS.gene51646:CDS |
| TTACTTATAATGGAACCATA+TGG | 0.538421 | 3.4:+4813245 | MS.gene51646:CDS |
| TCTTGCATGGAAGATAAGGT+TGG | 0.542256 | 3.4:+4812767 | MS.gene51646:CDS |
| GTCATTACTCCTTCACATAG+AGG | 0.545004 | 3.4:+4813635 | MS.gene51646:CDS |
| TGAATTTCTGATTCATGAGA+AGG | 0.547415 | 3.4:-4812057 | None:intergenic |
| ATGGTGAACACTGTAACAAT+AGG | 0.551113 | 3.4:-4812801 | None:intergenic |
| ATTGAAACAAAATCCAAGAT+GGG | 0.551532 | 3.4:+4811999 | MS.gene51646:CDS |
| CAATATTAATGTCAATCGAA+CGG | 0.554950 | 3.4:-4812203 | None:intergenic |
| TAGTTGTAATATGCAGCCCT+TGG | 0.555414 | 3.4:-4813443 | None:intergenic |
| ACTCCTTCACATAGAGGTGG+TGG | 0.555530 | 3.4:+4813641 | MS.gene51646:CDS |
| CTTATGTTTCAAAGTCTCCA+AGG | 0.559379 | 3.4:+4813426 | MS.gene51646:CDS |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.4:+4813176 | MS.gene51646:CDS |
| GATTGGTGTGCAAATTAGAG+TGG | 0.569189 | 3.4:+4812104 | MS.gene51646:CDS |
| ACATACAATACATGGTCAAG+TGG | 0.580747 | 3.4:+4813333 | MS.gene51646:CDS |
| TTATGTTTCAAAGTCTCCAA+GGG | 0.584994 | 3.4:+4813427 | MS.gene51646:CDS |
| GTGCAAATTAGAGTGGTTAG+TGG | 0.589648 | 3.4:+4812111 | MS.gene51646:CDS |
| ATAGGGATCTTGATATAGGA+AGG | 0.589814 | 3.4:+4813465 | MS.gene51646:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.4:+4812129 | MS.gene51646:CDS |
| ACCTTATAGATGTAAATGGA+AGG | 0.593956 | 3.4:+4812667 | MS.gene51646:CDS |
| GAATCTGCTTGGATTCAGGC+TGG | 0.594184 | 3.4:+4812231 | MS.gene51646:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.597018 | 3.4:+4813509 | MS.gene51646:CDS |
| GTGAACACTGTAACAATAGG+TGG | 0.602884 | 3.4:-4812798 | None:intergenic |
| GGAATAGGCGGTCACATCAG+TGG | 0.603755 | 3.4:+4812582 | MS.gene51646:intron |
| GGAGTAATGACATTTAGTAG+AGG | 0.607260 | 3.4:-4813623 | None:intergenic |
| GAAGGTGTCATAATGAGAAG+AGG | 0.607937 | 3.4:-4812039 | None:intergenic |
| CTTATAATGGAACCATATGG+TGG | 0.625352 | 3.4:+4813248 | MS.gene51646:CDS |
| CATACAATACATGGTCAAGT+GGG | 0.625707 | 3.4:+4813334 | MS.gene51646:CDS |
| ATTCCACCACCTCTATGTGA+AGG | 0.628617 | 3.4:-4813644 | None:intergenic |
| TAGACCATGTTTCCTAAACA+AGG | 0.647398 | 3.4:-4812619 | None:intergenic |
| TAAGGTAAGCATCAACAACA+TGG | 0.652825 | 3.4:-4812650 | None:intergenic |
| ATAGGCGGTCACATCAGTGG+AGG | 0.689151 | 3.4:+4812585 | MS.gene51646:intron |
| ATTACTCCTTCACATAGAGG+TGG | 0.692038 | 3.4:+4813638 | MS.gene51646:CDS |
| AAGGAAGAGAGAATTAAACA+TGG | 0.696240 | 3.4:-4812955 | None:intergenic |
| CAAATTAGAGTGGTTAGTGG+TGG | 0.705025 | 3.4:+4812114 | MS.gene51646:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAATAGAAAATCAAT+GGG | + | chr3.4:4812690-4812709 | MS.gene51646:CDS | 15.0% |
| !! | GATTCTTAATAGAAAATCAA+TGG | + | chr3.4:4812689-4812708 | MS.gene51646:CDS | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.4:4812177-4812196 | None:intergenic | 25.0% |
| ! | ATTGAAACAAAATCCAAGAT+GGG | + | chr3.4:4811999-4812018 | MS.gene51646:CDS | 25.0% |
| ! | CAATATTAATGTCAATCGAA+CGG | - | chr3.4:4812206-4812225 | None:intergenic | 25.0% |
| ! | CACATTAGCATTACTTATAA+TGG | + | chr3.4:4813235-4813254 | MS.gene51646:CDS | 25.0% |
| !! | ATTCATAAAATTCTGGAAGA+AGG | + | chr3.4:4812822-4812841 | MS.gene51646:CDS | 25.0% |
| !! | TTAAGTTTTCTTTTCTTCCA+AGG | - | chr3.4:4812977-4812996 | None:intergenic | 25.0% |
| !! | TTACTTATAATGGAACCATA+TGG | + | chr3.4:4813245-4813264 | MS.gene51646:CDS | 25.0% |
| !! | TTCTTCCAGAATTTTATGAA+TGG | - | chr3.4:4812823-4812842 | None:intergenic | 25.0% |
| !!! | ATTAAGTTGTTTTTCCAAAC+AGG | + | chr3.4:4812543-4812562 | MS.gene51646:intron | 25.0% |
| !!! | TTAAGTTGTTTTTCCAAACA+GGG | + | chr3.4:4812544-4812563 | MS.gene51646:intron | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.4:4813461-4813480 | MS.gene51646:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.4:4812140-4812159 | None:intergenic | 30.0% | |
| AAGGAAGAGAGAATTAAACA+TGG | - | chr3.4:4812958-4812977 | None:intergenic | 30.0% | |
| ACCTTATAGATGTAAATGGA+AGG | + | chr3.4:4812667-4812686 | MS.gene51646:CDS | 30.0% | |
| AGAATAGTTGCTCAAACTAA+TGG | + | chr3.4:4812909-4812928 | MS.gene51646:CDS | 30.0% | |
| AGCACAAGCAAATTATAAGA+CGG | + | chr3.4:4812352-4812371 | MS.gene51646:intron | 30.0% | |
| AGTCCAACAAACATATAAAG+TGG | + | chr3.4:4813375-4813394 | MS.gene51646:CDS | 30.0% | |
| ATATTGCAAATGAATCTGCT+TGG | + | chr3.4:4812220-4812239 | MS.gene51646:CDS | 30.0% | |
| ATGTTTAATTCTCTCTTCCT+TGG | + | chr3.4:4812957-4812976 | MS.gene51646:CDS | 30.0% | |
| CATCCACTTTATATGTTTGT+TGG | - | chr3.4:4813381-4813400 | None:intergenic | 30.0% | |
| CATTGAAACAAAATCCAAGA+TGG | + | chr3.4:4811998-4812017 | MS.gene51646:CDS | 30.0% | |
| CTTGTACAACATACAATACA+TGG | + | chr3.4:4813325-4813344 | MS.gene51646:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.4:4812178-4812197 | None:intergenic | 30.0% | |
| GCTTACCTTATAGATGTAAA+TGG | + | chr3.4:4812663-4812682 | MS.gene51646:CDS | 30.0% | |
| GTTCACCATTCATAAAATTC+TGG | + | chr3.4:4812815-4812834 | MS.gene51646:CDS | 30.0% | |
| TCCTTCCATTTACATCTATA+AGG | - | chr3.4:4812671-4812690 | None:intergenic | 30.0% | |
| TGAATTTCTGATTCATGAGA+AGG | - | chr3.4:4812060-4812079 | None:intergenic | 30.0% | |
| TTATGTTTCAAAGTCTCCAA+GGG | + | chr3.4:4813427-4813446 | MS.gene51646:CDS | 30.0% | |
| TTCAGAATAGCTACTAACTA+TGG | - | chr3.4:4811923-4811942 | None:intergenic | 30.0% | |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.4:4813594-4813613 | None:intergenic | 30.0% |
| ! | CTCTTTTACATAGTCAGATT+TGG | - | chr3.4:4813162-4813181 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.4:4813593-4813612 | None:intergenic | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+AGG | + | chr3.4:4813068-4813087 | MS.gene51646:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.4:4812707-4812726 | MS.gene51646:CDS | 30.0% |
| !!! | GATAGACACTGTTTTTTCAT+TGG | - | chr3.4:4811947-4811966 | None:intergenic | 30.0% |
| AAATGAATCTGCTTGGATTC+AGG | + | chr3.4:4812227-4812246 | MS.gene51646:CDS | 35.0% | |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.4:4813042-4813061 | MS.gene51646:CDS | 35.0% | |
| AAGTCAAACTAATTCACCTG+AGG | - | chr3.4:4812316-4812335 | None:intergenic | 35.0% | |
| AATCTCTTTGCTGCATAGTA+TGG | - | chr3.4:4812088-4812107 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCCTGAATT+AGG | + | chr3.4:4813008-4813027 | MS.gene51646:CDS | 35.0% | |
| ACATACAATACATGGTCAAG+TGG | + | chr3.4:4813333-4813352 | MS.gene51646:CDS | 35.0% | |
| AGTCAAACTAATTCACCTGA+GGG | - | chr3.4:4812315-4812334 | None:intergenic | 35.0% | |
| ATACTATGCAGCAAAGAGAT+TGG | + | chr3.4:4812087-4812106 | MS.gene51646:CDS | 35.0% | |
| ATAGGGATCTTGATATAGGA+AGG | + | chr3.4:4813465-4813484 | MS.gene51646:CDS | 35.0% | |
| ATGAGAGTTTCCCTGAATTA+GGG | + | chr3.4:4813009-4813028 | MS.gene51646:CDS | 35.0% | |
| ATGGTGAACACTGTAACAAT+AGG | - | chr3.4:4812804-4812823 | None:intergenic | 35.0% | |
| CATACAATACATGGTCAAGT+GGG | + | chr3.4:4813334-4813353 | MS.gene51646:CDS | 35.0% | |
| CCACAAAACTCATTCATAGA+TGG | + | chr3.4:4812847-4812866 | MS.gene51646:CDS | 35.0% | |
| CTGTTAAGCAAGAGTTCTAT+AGG | - | chr3.4:4813110-4813129 | None:intergenic | 35.0% | |
| CTTATGTTTCAAAGTCTCCA+AGG | + | chr3.4:4813426-4813445 | MS.gene51646:CDS | 35.0% | |
| GGAGTAATGACATTTAGTAG+AGG | - | chr3.4:4813626-4813645 | None:intergenic | 35.0% | |
| GGCTGCATATTACAACTATA+GGG | + | chr3.4:4813448-4813467 | MS.gene51646:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGAT+TGG | + | chr3.4:4813544-4813563 | MS.gene51646:CDS | 35.0% | |
| TAAGGTAAGCATCAACAACA+TGG | - | chr3.4:4812653-4812672 | None:intergenic | 35.0% | |
| TAGACCATGTTTCCTAAACA+AGG | - | chr3.4:4812622-4812641 | None:intergenic | 35.0% | |
| TCATTCTTGCATGGAAGATA+AGG | + | chr3.4:4812763-4812782 | MS.gene51646:CDS | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.4:4812285-4812304 | MS.gene51646:intron | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.4:4812289-4812308 | None:intergenic | 35.0% | |
| TTCAATGATTCCAACACTTG+TGG | - | chr3.4:4811985-4812004 | None:intergenic | 35.0% | |
| ! | CCATCTATGAATGAGTTTTG+TGG | - | chr3.4:4812850-4812869 | None:intergenic | 35.0% |
| ! | CTTATAATGGAACCATATGG+TGG | + | chr3.4:4813248-4813267 | MS.gene51646:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.4:4813595-4813614 | None:intergenic | 35.0% |
| ! | TACAAGTTTCCTTTTCTGTG+TGG | - | chr3.4:4813311-4813330 | None:intergenic | 35.0% |
| ! | TTCCTTTTCTGTGTGGAAAA+GGG | - | chr3.4:4813304-4813323 | None:intergenic | 35.0% |
| ! | TTTCCTTTTCTGTGTGGAAA+AGG | - | chr3.4:4813305-4813324 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.4:4813192-4813211 | MS.gene51646:CDS | 35.0% |
| !! | AGTCAGATTTGGCTTTGAAT+GGG | - | chr3.4:4813151-4813170 | None:intergenic | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.4:4813428-4813447 | None:intergenic | 35.0% |
| !! | TAGTCAGATTTGGCTTTGAA+TGG | - | chr3.4:4813152-4813171 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.4:4813585-4813604 | MS.gene51646:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.4:4812706-4812725 | MS.gene51646:CDS | 35.0% |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.4:4813191-4813210 | None:intergenic | 40.0% | |
| ATCCTTGTATCCACAAGTGT+TGG | + | chr3.4:4811972-4811991 | MS.gene51646:CDS | 40.0% | |
| ATTACTCCTTCACATAGAGG+TGG | + | chr3.4:4813638-4813657 | MS.gene51646:CDS | 40.0% | |
| CAAATTAGAGTGGTTAGTGG+TGG | + | chr3.4:4812114-4812133 | MS.gene51646:CDS | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.4:4813508-4813527 | MS.gene51646:CDS | 40.0% | |
| GATTGGTGTGCAAATTAGAG+TGG | + | chr3.4:4812104-4812123 | MS.gene51646:CDS | 40.0% | |
| GGGCTGCATATTACAACTAT+AGG | + | chr3.4:4813447-4813466 | MS.gene51646:CDS | 40.0% | |
| GTCAAACTAATTCACCTGAG+GGG | - | chr3.4:4812314-4812333 | None:intergenic | 40.0% | |
| GTCATTACTCCTTCACATAG+AGG | + | chr3.4:4813635-4813654 | MS.gene51646:CDS | 40.0% | |
| GTGAACACTGTAACAATAGG+TGG | - | chr3.4:4812801-4812820 | None:intergenic | 40.0% | |
| GTGCAAATTAGAGTGGTTAG+TGG | + | chr3.4:4812111-4812130 | MS.gene51646:CDS | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.4:4813509-4813528 | MS.gene51646:CDS | 40.0% | |
| TAGTTGTAATATGCAGCCCT+TGG | - | chr3.4:4813446-4813465 | None:intergenic | 40.0% | |
| TCACTCATTCTTCCACCATA+TGG | - | chr3.4:4813263-4813282 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.4:4813185-4813204 | None:intergenic | 40.0% | |
| TCTTGCATGGAAGATAAGGT+TGG | + | chr3.4:4812767-4812786 | MS.gene51646:CDS | 40.0% | |
| TGTCTTGCAACCCTAATTCA+GGG | - | chr3.4:4813022-4813041 | None:intergenic | 40.0% | |
| TTCCAACACTTGTGGATACA+AGG | - | chr3.4:4811977-4811996 | None:intergenic | 40.0% | |
| TTGTCTTGCAACCCTAATTC+AGG | - | chr3.4:4813023-4813042 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.4:4813185-4813204 | MS.gene51646:CDS | 40.0% |
| ! | ATCCCTTTTCCACACAGAAA+AGG | + | chr3.4:4813299-4813318 | MS.gene51646:CDS | 40.0% |
| ! | GGTACCTTGTTTAGGAAACA+TGG | + | chr3.4:4812615-4812634 | MS.gene51646:CDS | 40.0% |
| ! | GTGGATTTGGTACCTTGTTT+AGG | + | chr3.4:4812607-4812626 | MS.gene51646:CDS | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.4:4813527-4813546 | MS.gene51646:CDS | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.4:4813526-4813545 | MS.gene51646:CDS | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.4:4812042-4812061 | None:intergenic | 40.0% |
| !!! | GATGTTTTTTGGGCCATAAG+AGG | + | chr3.4:4812717-4812736 | MS.gene51646:CDS | 40.0% |
| !!! | GTTTTGGAGTCATTCTTGCA+TGG | + | chr3.4:4812754-4812773 | MS.gene51646:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.4:4813176-4813195 | MS.gene51646:CDS | 45.0% | |
| AAAGTTCATGGATTCCCCTC+AGG | + | chr3.4:4812297-4812316 | MS.gene51646:intron | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.4:4812129-4812148 | MS.gene51646:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.4:4812243-4812262 | MS.gene51646:CDS | 45.0% | |
| ATTCCACCACCTCTATGTGA+AGG | - | chr3.4:4813647-4813666 | None:intergenic | 45.0% | |
| CTTGATGAGTTCACCCATCT+TGG | - | chr3.4:4812015-4812034 | None:intergenic | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.4:4813507-4813526 | MS.gene51646:CDS | 45.0% | |
| !!! | GGCTTTGAATGGGCTTTTGT+AGG | - | chr3.4:4813141-4813160 | None:intergenic | 45.0% |
| !!! | GTTTTTTGGGCCATAAGAGG+AGG | + | chr3.4:4812720-4812739 | MS.gene51646:CDS | 45.0% |
| ACTCCTTCACATAGAGGTGG+TGG | + | chr3.4:4813641-4813660 | MS.gene51646:CDS | 50.0% | |
| AGCAGCACTACCTCCTCTTA+TGG | - | chr3.4:4812733-4812752 | None:intergenic | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.4:4812231-4812250 | MS.gene51646:CDS | 50.0% | |
| ! | CACATCAGTGGAGGTGGATT+TGG | + | chr3.4:4812594-4812613 | MS.gene51646:CDS | 50.0% |
| !! | GGAGGTAGTGCTGCTAGTTT+TGG | + | chr3.4:4812738-4812757 | MS.gene51646:CDS | 50.0% |
| !! | TTGTGTCCGAGCGTTGGAAT+AGG | + | chr3.4:4812567-4812586 | MS.gene51646:intron | 50.0% |
| ATAGGCGGTCACATCAGTGG+AGG | + | chr3.4:4812585-4812604 | MS.gene51646:intron | 55.0% | |
| GGAATAGGCGGTCACATCAG+TGG | + | chr3.4:4812582-4812601 | MS.gene51646:intron | 55.0% | |
| TGACCGCCTATTCCAACGCT+CGG | - | chr3.4:4812576-4812595 | None:intergenic | 55.0% | |
| !! | TGTCCGAGCGTTGGAATAGG+CGG | + | chr3.4:4812570-4812589 | MS.gene51646:intron | 55.0% |
| ACAGGGTTGTGTCCGAGCGT+TGG | + | chr3.4:4812561-4812580 | MS.gene51646:intron | 60.0% | |
| CGCTCGGACACAACCCTGTT+TGG | - | chr3.4:4812560-4812579 | None:intergenic | 60.0% | |
| GGCGGTCACATCAGTGGAGG+TGG | + | chr3.4:4812588-4812607 | MS.gene51646:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 4811904 | 4813673 | 4811904 | ID=MS.gene51646 |
| chr3.4 | mRNA | 4811904 | 4813673 | 4811904 | ID=MS.gene51646.t1;Parent=MS.gene51646 |
| chr3.4 | exon | 4811904 | 4812264 | 4811904 | ID=MS.gene51646.t1.exon1;Parent=MS.gene51646.t1 |
| chr3.4 | CDS | 4811904 | 4812264 | 4811904 | ID=cds.MS.gene51646.t1;Parent=MS.gene51646.t1 |
| chr3.4 | exon | 4812589 | 4813673 | 4812589 | ID=MS.gene51646.t1.exon2;Parent=MS.gene51646.t1 |
| chr3.4 | CDS | 4812589 | 4813673 | 4812589 | ID=cds.MS.gene51646.t1;Parent=MS.gene51646.t1 |
| Gene Sequence |
| Protein sequence |
