Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56279.t1 | XP_003598276.1 | 88.7 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 5.90E-241 | 843.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56279.t1 | Q9SUC6 | 52.6 | 477 | 206 | 8 | 8 | 468 | 11 | 483 | 1.4e-142 | 507.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56279.t1 | G7J0C2 | 88.7 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 4.3e-241 | 843.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28742 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene047977 | PPI |
| MS.gene56279 | MS.gene36444 | PPI |
| MS.gene36445 | MS.gene56279 | PPI |
| MS.gene36439 | MS.gene56279 | PPI |
| MS.gene35114 | MS.gene56279 | PPI |
| MS.gene028369 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene028353 | PPI |
| MS.gene028347 | MS.gene56279 | PPI |
| MS.gene35106 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene028354 | PPI |
| MS.gene28727 | MS.gene56279 | PPI |
| MS.gene35104 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene35103 | PPI |
| MS.gene35105 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene36437 | PPI |
| MS.gene36438 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene028357 | PPI |
| MS.gene003357 | MS.gene56279 | PPI |
| MS.gene28724 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene028346 | PPI |
| MS.gene56279 | MS.gene36435 | PPI |
| MS.gene02146 | MS.gene56279 | PPI |
| MS.gene56279 | MS.gene28725 | PPI |
| MS.gene35113 | MS.gene56279 | PPI |
| MS.gene36421 | MS.gene56279 | PPI |
| MS.gene28726 | MS.gene56279 | PPI |
| MS.gene35083 | MS.gene56279 | PPI |
| MS.gene28717 | MS.gene56279 | PPI |
| MS.gene28723 | MS.gene56279 | PPI |
| MS.gene028352 | MS.gene56279 | PPI |
| MS.gene35102 | MS.gene56279 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56279.t1 | MTR_3g009760 | 88.655 | 476 | 46 | 1 | 1 | 468 | 1 | 476 | 0.0 | 847 |
| MS.gene56279.t1 | MTR_3g009820 | 88.235 | 476 | 48 | 1 | 1 | 468 | 1 | 476 | 0.0 | 841 |
| MS.gene56279.t1 | MTR_3g009880 | 84.728 | 478 | 63 | 2 | 1 | 470 | 1 | 476 | 0.0 | 806 |
| MS.gene56279.t1 | MTR_2g031390 | 50.538 | 465 | 211 | 7 | 19 | 468 | 23 | 483 | 6.22e-169 | 487 |
| MS.gene56279.t1 | MTR_2g031420 | 53.333 | 480 | 205 | 8 | 1 | 467 | 8 | 481 | 7.79e-168 | 483 |
| MS.gene56279.t1 | MTR_2g031460 | 52.795 | 483 | 205 | 8 | 1 | 468 | 8 | 482 | 1.06e-167 | 483 |
| MS.gene56279.t1 | MTR_4g094488 | 51.340 | 485 | 214 | 6 | 3 | 467 | 7 | 489 | 2.67e-166 | 480 |
| MS.gene56279.t1 | MTR_4g094495 | 52.147 | 489 | 209 | 6 | 2 | 467 | 6 | 492 | 2.14e-165 | 478 |
| MS.gene56279.t1 | MTR_4g094492 | 51.951 | 487 | 209 | 8 | 2 | 467 | 6 | 488 | 1.36e-164 | 476 |
| MS.gene56279.t1 | MTR_4g094520 | 49.378 | 482 | 225 | 9 | 1 | 468 | 10 | 486 | 6.70e-164 | 474 |
| MS.gene56279.t1 | MTR_4g094478 | 50.104 | 479 | 222 | 5 | 4 | 467 | 11 | 487 | 5.09e-163 | 472 |
| MS.gene56279.t1 | MTR_4g094518 | 53.813 | 459 | 191 | 9 | 25 | 467 | 30 | 483 | 1.85e-159 | 462 |
| MS.gene56279.t1 | MTR_4g094480 | 48.347 | 484 | 230 | 7 | 2 | 467 | 6 | 487 | 3.25e-156 | 454 |
| MS.gene56279.t1 | MTR_2g031560 | 46.570 | 481 | 242 | 5 | 4 | 472 | 3 | 480 | 5.05e-156 | 454 |
| MS.gene56279.t1 | MTR_4g094418 | 55.814 | 387 | 154 | 4 | 98 | 467 | 1 | 387 | 1.53e-154 | 452 |
| MS.gene56279.t1 | MTR_4g094498 | 48.643 | 479 | 225 | 6 | 7 | 467 | 12 | 487 | 7.59e-154 | 449 |
| MS.gene56279.t1 | MTR_2g031590 | 46.598 | 485 | 237 | 9 | 1 | 467 | 5 | 485 | 7.09e-151 | 441 |
| MS.gene56279.t1 | MTR_4g057990 | 43.882 | 474 | 252 | 4 | 1 | 465 | 4 | 472 | 5.44e-139 | 410 |
| MS.gene56279.t1 | MTR_2g031510 | 44.017 | 468 | 243 | 6 | 17 | 467 | 28 | 493 | 4.77e-138 | 408 |
| MS.gene56279.t1 | MTR_2g031490 | 43.532 | 487 | 251 | 9 | 1 | 467 | 9 | 491 | 1.98e-137 | 406 |
| MS.gene56279.t1 | MTR_4g091190 | 44.885 | 479 | 235 | 9 | 7 | 465 | 9 | 478 | 4.73e-136 | 402 |
| MS.gene56279.t1 | MTR_4g091200 | 44.770 | 478 | 243 | 7 | 1 | 465 | 7 | 476 | 3.14e-135 | 400 |
| MS.gene56279.t1 | MTR_4g091150 | 46.389 | 457 | 226 | 8 | 24 | 465 | 37 | 489 | 5.45e-134 | 397 |
| MS.gene56279.t1 | MTR_4g088355 | 45.140 | 463 | 223 | 8 | 24 | 465 | 31 | 483 | 3.80e-133 | 395 |
| MS.gene56279.t1 | MTR_6g017205 | 44.118 | 476 | 241 | 8 | 7 | 465 | 9 | 476 | 5.33e-132 | 392 |
| MS.gene56279.t1 | MTR_4g094515 | 45.267 | 486 | 243 | 9 | 1 | 467 | 622 | 1103 | 4.04e-131 | 407 |
| MS.gene56279.t1 | MTR_4g094515 | 44.979 | 478 | 235 | 11 | 11 | 467 | 23 | 493 | 1.28e-124 | 390 |
| MS.gene56279.t1 | MTR_4g091170 | 45.545 | 404 | 191 | 5 | 73 | 465 | 107 | 492 | 7.24e-126 | 378 |
| MS.gene56279.t1 | MTR_2g023590 | 46.324 | 408 | 202 | 6 | 70 | 464 | 12 | 415 | 2.78e-122 | 365 |
| MS.gene56279.t1 | MTR_3g009850 | 87.640 | 178 | 22 | 0 | 291 | 468 | 5 | 182 | 5.08e-112 | 330 |
| MS.gene56279.t1 | MTR_1g076830 | 41.686 | 427 | 234 | 6 | 54 | 466 | 60 | 485 | 2.50e-104 | 322 |
| MS.gene56279.t1 | MTR_2g031600 | 45.528 | 246 | 116 | 6 | 1 | 231 | 1 | 243 | 4.31e-62 | 207 |
| MS.gene56279.t1 | MTR_2g031600 | 52.212 | 113 | 51 | 2 | 356 | 467 | 243 | 353 | 6.33e-33 | 129 |
| MS.gene56279.t1 | MTR_2g084370 | 73.786 | 103 | 13 | 1 | 122 | 224 | 1 | 89 | 6.90e-46 | 155 |
| MS.gene56279.t1 | MTR_2g084405 | 78.750 | 80 | 9 | 1 | 122 | 193 | 1 | 80 | 3.66e-30 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56279.t1 | AT4G20860 | 52.542 | 472 | 204 | 8 | 14 | 469 | 17 | 484 | 1.81e-169 | 488 |
| MS.gene56279.t1 | AT5G44360 | 51.527 | 491 | 202 | 13 | 1 | 469 | 9 | 485 | 3.51e-163 | 472 |
| MS.gene56279.t1 | AT5G44360 | 51.527 | 491 | 202 | 13 | 1 | 469 | 25 | 501 | 3.93e-163 | 472 |
| MS.gene56279.t1 | AT5G44400 | 51.967 | 483 | 208 | 11 | 4 | 465 | 7 | 486 | 1.30e-162 | 471 |
| MS.gene56279.t1 | AT1G30760 | 51.183 | 465 | 206 | 9 | 20 | 467 | 29 | 489 | 2.76e-158 | 459 |
| MS.gene56279.t1 | AT1G30760 | 51.183 | 465 | 206 | 9 | 20 | 467 | 47 | 507 | 3.01e-158 | 460 |
| MS.gene56279.t1 | AT2G34790 | 48.770 | 488 | 218 | 9 | 5 | 469 | 11 | 489 | 3.82e-158 | 459 |
| MS.gene56279.t1 | AT5G44390 | 50.317 | 473 | 215 | 11 | 13 | 468 | 24 | 493 | 1.55e-154 | 450 |
| MS.gene56279.t1 | AT4G20820 | 46.383 | 470 | 230 | 9 | 8 | 466 | 19 | 477 | 7.51e-145 | 425 |
| MS.gene56279.t1 | AT5G44440 | 45.114 | 481 | 243 | 9 | 2 | 466 | 5 | 480 | 6.95e-144 | 422 |
| MS.gene56279.t1 | AT5G44440 | 45.114 | 481 | 243 | 9 | 2 | 466 | 75 | 550 | 1.01e-142 | 422 |
| MS.gene56279.t1 | AT5G44380 | 48.017 | 479 | 227 | 11 | 9 | 468 | 17 | 492 | 7.09e-138 | 407 |
| MS.gene56279.t1 | AT5G44380 | 48.017 | 479 | 227 | 11 | 9 | 468 | 57 | 532 | 1.75e-137 | 408 |
| MS.gene56279.t1 | AT5G44410 | 44.937 | 474 | 239 | 8 | 7 | 466 | 14 | 479 | 1.19e-133 | 397 |
| MS.gene56279.t1 | AT1G26400 | 44.703 | 472 | 246 | 6 | 8 | 467 | 10 | 478 | 1.07e-132 | 394 |
| MS.gene56279.t1 | AT1G26420 | 45.188 | 478 | 231 | 10 | 8 | 465 | 10 | 476 | 1.25e-132 | 394 |
| MS.gene56279.t1 | AT1G26380 | 44.513 | 483 | 239 | 9 | 2 | 465 | 4 | 476 | 1.55e-132 | 394 |
| MS.gene56279.t1 | AT1G26390 | 47.917 | 432 | 206 | 7 | 48 | 465 | 52 | 478 | 2.41e-132 | 393 |
| MS.gene56279.t1 | AT1G26410 | 47.796 | 431 | 206 | 7 | 48 | 465 | 76 | 500 | 7.58e-132 | 392 |
| MS.gene56279.t1 | AT1G30710 | 42.708 | 480 | 252 | 9 | 4 | 467 | 11 | 483 | 7.71e-131 | 389 |
| MS.gene56279.t1 | AT1G30700 | 45.435 | 460 | 227 | 9 | 24 | 467 | 26 | 477 | 2.44e-130 | 388 |
| MS.gene56279.t1 | AT1G34575 | 42.324 | 482 | 243 | 11 | 4 | 465 | 11 | 477 | 2.22e-129 | 385 |
| MS.gene56279.t1 | AT1G11770 | 40.756 | 476 | 266 | 8 | 4 | 467 | 11 | 482 | 3.37e-125 | 375 |
| MS.gene56279.t1 | AT1G01980 | 43.220 | 472 | 244 | 10 | 15 | 467 | 21 | 487 | 3.17e-124 | 372 |
| MS.gene56279.t1 | AT4G20840 | 42.576 | 458 | 244 | 8 | 24 | 467 | 36 | 488 | 2.76e-123 | 370 |
| MS.gene56279.t1 | AT4G20800 | 43.640 | 456 | 237 | 9 | 24 | 465 | 27 | 476 | 6.50e-123 | 369 |
| MS.gene56279.t1 | AT1G30730 | 42.072 | 473 | 259 | 6 | 8 | 467 | 10 | 480 | 2.60e-122 | 367 |
| MS.gene56279.t1 | AT4G20830 | 42.641 | 462 | 248 | 8 | 19 | 467 | 32 | 489 | 4.41e-119 | 359 |
| MS.gene56279.t1 | AT2G34810 | 42.463 | 471 | 250 | 7 | 15 | 467 | 24 | 491 | 4.86e-119 | 359 |
| MS.gene56279.t1 | AT4G20830 | 42.641 | 462 | 248 | 8 | 19 | 467 | 32 | 489 | 2.11e-118 | 358 |
| MS.gene56279.t1 | AT1G30720 | 40.171 | 468 | 265 | 6 | 13 | 467 | 16 | 481 | 3.50e-117 | 354 |
| MS.gene56279.t1 | AT1G30740 | 40.764 | 471 | 262 | 8 | 11 | 467 | 14 | 481 | 2.14e-116 | 352 |
| MS.gene56279.t1 | AT1G30740 | 40.764 | 471 | 262 | 8 | 11 | 467 | 17 | 484 | 2.81e-116 | 352 |
Find 81 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGATTTAGAAGGTATT+TGG | 0.185286 | 3.1:+5066682 | MS.gene56279:CDS |
| GCTTACCTAATAGATGTAAA+TGG | 0.192403 | 3.1:+5066153 | MS.gene56279:CDS |
| TCTTGCATGGAAGATAAATT+TGG | 0.222820 | 3.1:+5066257 | MS.gene56279:CDS |
| TCCATGAACTTTACTTGCTT+TGG | 0.270151 | 3.1:-5065748 | None:intergenic |
| CGCATCAGCATTACTTATAA+TGG | 0.276583 | 3.1:+5066725 | MS.gene56279:CDS |
| CGATGAATCTGCTTGGATTC+AGG | 0.282488 | 3.1:+5065689 | MS.gene56279:CDS |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.1:+5066228 | MS.gene56279:CDS |
| CGTCCACTTTATATGCTTGT+TGG | 0.289760 | 3.1:-5066868 | None:intergenic |
| AAAGAGAGTTAAACATTGTT+TGG | 0.291401 | 3.1:-5066440 | None:intergenic |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.1:+5066937 | MS.gene56279:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.1:-5065524 | None:intergenic |
| CTTGATGAATTCAGCCATCT+TGG | 0.311842 | 3.1:-5065474 | None:intergenic |
| AATGAGAGTTTCCTTGAGTT+AGG | 0.320152 | 3.1:+5066498 | MS.gene56279:CDS |
| CACATCAGTGGAGGAGGATT+TGG | 0.323062 | 3.1:+5066084 | MS.gene56279:CDS |
| CTGTTCAGCAAGAGTTCTAT+AGG | 0.327466 | 3.1:-5066597 | None:intergenic |
| TCCAAAGCAAGTAAAGTTCA+TGG | 0.328871 | 3.1:+5065747 | MS.gene56279:CDS |
| TTTCCTTGAGTTAGGGTTGC+AGG | 0.336327 | 3.1:+5066506 | MS.gene56279:CDS |
| CAATACTTGTGGATACAATA+TGG | 0.349912 | 3.1:-5065433 | None:intergenic |
| TCTAAATCAGCTTCTGGTAT+AGG | 0.352720 | 3.1:-5066672 | None:intergenic |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.1:+5065705 | MS.gene56279:CDS |
| ATACCTTCTAAATCAGCTTC+TGG | 0.399342 | 3.1:-5066678 | None:intergenic |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.1:-5066915 | None:intergenic |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.1:+5066938 | MS.gene56279:CDS |
| TAATCAGACTTGGCTATGAA+TGG | 0.426890 | 3.1:-5066639 | None:intergenic |
| CATATTGTATCCACAAGTAT+TGG | 0.430537 | 3.1:+5065434 | MS.gene56279:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.1:-5065637 | None:intergenic |
| CTATGTATTGCCACCTATGA+AGG | 0.438010 | 3.1:-5066347 | None:intergenic |
| CTTACCTAATAGATGTAAAT+GGG | 0.446115 | 3.1:+5066154 | MS.gene56279:CDS |
| ATACCAGAAGCTGATTTAGA+AGG | 0.450654 | 3.1:+5066675 | MS.gene56279:CDS |
| AGAATAGTTGCTCAAAATAG+TGG | 0.457619 | 3.1:+5066399 | MS.gene56279:CDS |
| GAGCAACCAAGCTCCTTCAT+AGG | 0.458493 | 3.1:+5066334 | MS.gene56279:CDS |
| ATGAGAGTTTCCTTGAGTTA+GGG | 0.464989 | 3.1:+5066499 | MS.gene56279:CDS |
| AAGACTGCATTGAAATGAGT+TGG | 0.465070 | 3.1:+5066532 | MS.gene56279:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.1:+5066951 | MS.gene56279:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.1:-5065636 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.1:-5065599 | None:intergenic |
| ATCTTGCCGATGAATCTGCT+TGG | 0.489037 | 3.1:+5065682 | MS.gene56279:CDS |
| TTCAGTGATTCCAATACTTG+TGG | 0.491607 | 3.1:-5065444 | None:intergenic |
| TTCAGAAAAGCTACTACCTA+TGG | 0.493468 | 3.1:-5065376 | None:intergenic |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.1:-5065523 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.1:-5066933 | None:intergenic |
| CTCTTCTACATAATCAGACT+TGG | 0.507678 | 3.1:-5066649 | None:intergenic |
| AATCAGACTTGGCTATGAAT+GGG | 0.513621 | 3.1:-5066638 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.516266 | 3.1:+5066865 | MS.gene56279:CDS |
| ATTCTTGATAGAAAAGCAAT+GGG | 0.518994 | 3.1:+5066180 | MS.gene56279:CDS |
| GAGGATTTGGTACCTTGGTA+AGG | 0.527609 | 3.1:+5066097 | MS.gene56279:CDS |
| GTTCAACATTCAAAGAACAT+TGG | 0.536377 | 3.1:+5066305 | MS.gene56279:CDS |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.1:+5065759 | MS.gene56279:CDS |
| TGTTCAGCAAGAGTTCTATA+GGG | 0.539613 | 3.1:-5066596 | None:intergenic |
| ATGCAGTGCATGTTAACCAT+AGG | 0.544066 | 3.1:+5065360 | MS.gene56279:CDS |
| CACTGAAACAAAATCCAAGA+TGG | 0.547598 | 3.1:+5065460 | MS.gene56279:CDS |
| AATTCTTGATAGAAAAGCAA+TGG | 0.550485 | 3.1:+5066179 | MS.gene56279:CDS |
| TGGAGGAGGATTTGGTACCT+TGG | 0.552904 | 3.1:+5066092 | MS.gene56279:CDS |
| TTTCCTGCAACCCTAACTCA+AGG | 0.554409 | 3.1:-5066509 | None:intergenic |
| ACATACAATACTTTGTCAAG+TGG | 0.554747 | 3.1:+5066823 | MS.gene56279:CDS |
| ATGTTGAACACTGTAACAAT+AGG | 0.557897 | 3.1:-5066291 | None:intergenic |
| TTACTTATAATGGAGCCATA+TGG | 0.558832 | 3.1:+5066735 | MS.gene56279:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.1:-5065519 | None:intergenic |
| GGTGGTCACATCAGTGGAGG+AGG | 0.567357 | 3.1:+5066078 | MS.gene56279:intron |
| CTTATAATGGAGCCATATGG+TGG | 0.568422 | 3.1:+5066738 | MS.gene56279:CDS |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.1:+5065573 | MS.gene56279:CDS |
| TTGAACACTGTAACAATAGG+TGG | 0.572928 | 3.1:-5066288 | None:intergenic |
| CTGAATCCAAGCAGATTCAT+CGG | 0.574804 | 3.1:-5065688 | None:intergenic |
| GAATCTGCTTGGATTCAGGC+TGG | 0.577913 | 3.1:+5065693 | MS.gene56279:CDS |
| TTGCCACCTATGAAGGAGCT+TGG | 0.579989 | 3.1:-5066340 | None:intergenic |
| GGTACCTTGGTAAGGAAACA+TGG | 0.586233 | 3.1:+5066105 | MS.gene56279:CDS |
| CATACAATACTTTGTCAAGT+GGG | 0.588214 | 3.1:+5066824 | MS.gene56279:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.1:+5065591 | MS.gene56279:CDS |
| AAACTAATTAATTACCTGCA+GGG | 0.596070 | 3.1:-5065773 | None:intergenic |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.1:+5065566 | MS.gene56279:CDS |
| GGAGTAGGTGGTCACATCAG+TGG | 0.610281 | 3.1:+5066072 | MS.gene56279:intron |
| CAAGGGAGGGAATTCAGAAG+AGG | 0.618945 | 3.1:-5067113 | None:intergenic |
| CAAGATTAATGTCGATCGAA+CGG | 0.627262 | 3.1:-5065665 | None:intergenic |
| CAACCAAGCTCCTTCATAGG+TGG | 0.630946 | 3.1:+5066337 | MS.gene56279:CDS |
| AGAACTCTTGCTGAACAGAA+CGG | 0.634545 | 3.1:+5066602 | MS.gene56279:CDS |
| GAAGGTGTCATAATGAGAAG+AGG | 0.648177 | 3.1:-5065501 | None:intergenic |
| TTATAATGGAGCCATATGGT+GGG | 0.651843 | 3.1:+5066739 | MS.gene56279:CDS |
| TAGACCATGTTTCCTTACCA+AGG | 0.652475 | 3.1:-5066109 | None:intergenic |
| GTAGGTGGTCACATCAGTGG+AGG | 0.667986 | 3.1:+5066075 | MS.gene56279:intron |
| ATTCAAAGAACATTGGAACA+AGG | 0.678567 | 3.1:+5066312 | MS.gene56279:CDS |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.1:+5065576 | MS.gene56279:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTAATTAATTACCTGCA+GGG | - | chr3.1:5065776-5065795 | None:intergenic | 25.0% |
| ! | AAAGAGAGTTAAACATTGTT+TGG | - | chr3.1:5066443-5066462 | None:intergenic | 25.0% |
| ! | AATTCTTGATAGAAAAGCAA+TGG | + | chr3.1:5066179-5066198 | MS.gene56279:CDS | 25.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.1:5065639-5065658 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.1:5066033-5066052 | MS.gene56279:intron | 25.0% |
| ! | ATTCTTGATAGAAAAGCAAT+GGG | + | chr3.1:5066180-5066199 | MS.gene56279:CDS | 25.0% |
| ! | CTTACCTAATAGATGTAAAT+GGG | + | chr3.1:5066154-5066173 | MS.gene56279:CDS | 25.0% |
| !! | TTTTCCCATTTACATCTATT+AGG | - | chr3.1:5066161-5066180 | None:intergenic | 25.0% |
| !!! | CAATCAGTTTTATATATTGC+AGG | + | chr3.1:5066558-5066577 | MS.gene56279:CDS | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.1:5066951-5066970 | MS.gene56279:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.1:5065602-5065621 | None:intergenic | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.1:5066865-5066884 | MS.gene56279:CDS | 30.0% | |
| ACATACAATACTTTGTCAAG+TGG | + | chr3.1:5066823-5066842 | MS.gene56279:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.1:5066399-5066418 | MS.gene56279:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.1:5066938-5066957 | MS.gene56279:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.1:5065549-5065568 | MS.gene56279:CDS | 30.0% | |
| ATGTTGAACACTGTAACAAT+AGG | - | chr3.1:5066294-5066313 | None:intergenic | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.1:5066312-5066331 | MS.gene56279:CDS | 30.0% | |
| CAAACTAATTAATTACCTGC+AGG | - | chr3.1:5065777-5065796 | None:intergenic | 30.0% | |
| CAATACTTGTGGATACAATA+TGG | - | chr3.1:5065436-5065455 | None:intergenic | 30.0% | |
| CATACAATACTTTGTCAAGT+GGG | + | chr3.1:5066824-5066843 | MS.gene56279:CDS | 30.0% | |
| CATATTGTATCCACAAGTAT+TGG | + | chr3.1:5065434-5065453 | MS.gene56279:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.1:5065640-5065659 | None:intergenic | 30.0% | |
| GCTTACCTAATAGATGTAAA+TGG | + | chr3.1:5066153-5066172 | MS.gene56279:CDS | 30.0% | |
| GTTCAACATTCAAAGAACAT+TGG | + | chr3.1:5066305-5066324 | MS.gene56279:CDS | 30.0% | |
| TCTTGCATGGAAGATAAATT+TGG | + | chr3.1:5066257-5066276 | MS.gene56279:CDS | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.1:5065526-5065545 | None:intergenic | 30.0% | |
| ! | AACAAGTTTCCTTTTCTATG+TGG | - | chr3.1:5066801-5066820 | None:intergenic | 30.0% |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.1:5065550-5065569 | None:intergenic | 30.0% |
| ! | ATGTTTAACTCTCTTTTCCT+TGG | + | chr3.1:5066447-5066466 | MS.gene56279:CDS | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.1:5067084-5067103 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.1:5067083-5067102 | None:intergenic | 30.0% |
| ! | TTAAGTTTTCTTTTCCTCCA+AGG | - | chr3.1:5066467-5066486 | None:intergenic | 30.0% |
| ! | TTACTTATAATGGAGCCATA+TGG | + | chr3.1:5066735-5066754 | MS.gene56279:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.1:5066794-5066813 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.1:5066795-5066814 | None:intergenic | 30.0% |
| !! | AAGCTGATTTAGAAGGTATT+TGG | + | chr3.1:5066682-5066701 | MS.gene56279:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.1:5066197-5066216 | MS.gene56279:CDS | 30.0% |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.1:5066532-5066551 | MS.gene56279:CDS | 35.0% | |
| AATCAGACTTGGCTATGAAT+GGG | - | chr3.1:5066641-5066660 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCTTGAGTT+AGG | + | chr3.1:5066498-5066517 | MS.gene56279:CDS | 35.0% | |
| ATACCTTCTAAATCAGCTTC+TGG | - | chr3.1:5066681-5066700 | None:intergenic | 35.0% | |
| ATGAGAGTTTCCTTGAGTTA+GGG | + | chr3.1:5066499-5066518 | MS.gene56279:CDS | 35.0% | |
| CAAGATTAATGTCGATCGAA+CGG | - | chr3.1:5065668-5065687 | None:intergenic | 35.0% | |
| CACTGAAACAAAATCCAAGA+TGG | + | chr3.1:5065460-5065479 | MS.gene56279:CDS | 35.0% | |
| CGCATCAGCATTACTTATAA+TGG | + | chr3.1:5066725-5066744 | MS.gene56279:CDS | 35.0% | |
| CTCTTCTACATAATCAGACT+TGG | - | chr3.1:5066652-5066671 | None:intergenic | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.1:5065527-5065546 | None:intergenic | 35.0% | |
| CTTGTATCTATAAGCTCTCA+AGG | + | chr3.1:5065904-5065923 | MS.gene56279:intron | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.1:5066937-5066956 | MS.gene56279:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGTT+TGG | + | chr3.1:5067034-5067053 | MS.gene56279:intron | 35.0% | |
| TAATCAGACTTGGCTATGAA+TGG | - | chr3.1:5066642-5066661 | None:intergenic | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.1:5066936-5066955 | None:intergenic | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.1:5065747-5065766 | MS.gene56279:CDS | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.1:5065751-5065770 | None:intergenic | 35.0% | |
| TCTAAATCAGCTTCTGGTAT+AGG | - | chr3.1:5066675-5066694 | None:intergenic | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.1:5065522-5065541 | None:intergenic | 35.0% | |
| TGTTCAGCAAGAGTTCTATA+GGG | - | chr3.1:5066599-5066618 | None:intergenic | 35.0% | |
| TTCAGAAAAGCTACTACCTA+TGG | - | chr3.1:5065379-5065398 | None:intergenic | 35.0% | |
| TTCAGTGATTCCAATACTTG+TGG | - | chr3.1:5065447-5065466 | None:intergenic | 35.0% | |
| TTGAACACTGTAACAATAGG+TGG | - | chr3.1:5066291-5066310 | None:intergenic | 35.0% | |
| ! | ATACCAGAAGCTGATTTAGA+AGG | + | chr3.1:5066675-5066694 | MS.gene56279:CDS | 35.0% |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.1:5066789-5066808 | MS.gene56279:CDS | 35.0% |
| ! | GATAAACACTGTGTTTTCAC+TGG | - | chr3.1:5065403-5065422 | None:intergenic | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.1:5067085-5067104 | None:intergenic | 35.0% |
| ! | TTATAATGGAGCCATATGGT+GGG | + | chr3.1:5066739-5066758 | MS.gene56279:CDS | 35.0% |
| ! | TTTAACTCTCTTTTCCTTGG+AGG | + | chr3.1:5066450-5066469 | MS.gene56279:CDS | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.1:5066918-5066937 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.1:5067075-5067094 | MS.gene56279:intron | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.1:5066196-5066215 | MS.gene56279:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.1:5066207-5066226 | MS.gene56279:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.1:5066244-5066263 | MS.gene56279:CDS | 35.0% |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.1:5066051-5066070 | MS.gene56279:intron | 40.0% | |
| AGAACTCTTGCTGAACAGAA+CGG | + | chr3.1:5066602-5066621 | MS.gene56279:CDS | 40.0% | |
| ATGCAGTGCATGTTAACCAT+AGG | + | chr3.1:5065360-5065379 | MS.gene56279:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.1:5066050-5066069 | None:intergenic | 40.0% | |
| CGTCCACTTTATATGCTTGT+TGG | - | chr3.1:5066871-5066890 | None:intergenic | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.1:5066998-5067017 | MS.gene56279:intron | 40.0% | |
| CTATGTATTGCCACCTATGA+AGG | - | chr3.1:5066350-5066369 | None:intergenic | 40.0% | |
| CTGAATCCAAGCAGATTCAT+CGG | - | chr3.1:5065691-5065710 | None:intergenic | 40.0% | |
| CTGTTCAGCAAGAGTTCTAT+AGG | - | chr3.1:5066600-5066619 | None:intergenic | 40.0% | |
| CTTGATGAATTCAGCCATCT+TGG | - | chr3.1:5065477-5065496 | None:intergenic | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.1:5066999-5067018 | MS.gene56279:intron | 40.0% | |
| TAGACCATGTTTCCTTACCA+AGG | - | chr3.1:5066112-5066131 | None:intergenic | 40.0% | |
| ! | CTTATAATGGAGCCATATGG+TGG | + | chr3.1:5066738-5066757 | MS.gene56279:CDS | 40.0% |
| ! | TCACTCATTTTCCCACCATA+TGG | - | chr3.1:5066753-5066772 | None:intergenic | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.1:5067017-5067036 | MS.gene56279:intron | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.1:5067016-5067035 | MS.gene56279:intron | 40.0% |
| !! | ATATGTCCAAGTGTTGGAGT+AGG | + | chr3.1:5066057-5066076 | MS.gene56279:intron | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.1:5065504-5065523 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.1:5066210-5066229 | MS.gene56279:CDS | 40.0% |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.1:5065759-5065778 | MS.gene56279:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.1:5065591-5065610 | MS.gene56279:CDS | 45.0% | |
| ATCTTGCCGATGAATCTGCT+TGG | + | chr3.1:5065682-5065701 | MS.gene56279:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.1:5065705-5065724 | MS.gene56279:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.1:5065576-5065595 | MS.gene56279:CDS | 45.0% | |
| CGATGAATCTGCTTGGATTC+AGG | + | chr3.1:5065689-5065708 | MS.gene56279:CDS | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.1:5065566-5065585 | MS.gene56279:CDS | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.1:5066997-5067016 | MS.gene56279:intron | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.1:5066105-5066124 | MS.gene56279:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.1:5065573-5065592 | MS.gene56279:CDS | 45.0% | |
| TTTCCTGCAACCCTAACTCA+AGG | - | chr3.1:5066512-5066531 | None:intergenic | 45.0% | |
| TTTCCTTGAGTTAGGGTTGC+AGG | + | chr3.1:5066506-5066525 | MS.gene56279:CDS | 45.0% | |
| ! | GAGGATTTGGTACCTTGGTA+AGG | + | chr3.1:5066097-5066116 | MS.gene56279:CDS | 45.0% |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.1:5066228-5066247 | MS.gene56279:CDS | 45.0% |
| !! | GGCTATGAATGGGCTTTTGT+AGG | - | chr3.1:5066631-5066650 | None:intergenic | 45.0% |
| CAACCAAGCTCCTTCATAGG+TGG | + | chr3.1:5066337-5066356 | MS.gene56279:CDS | 50.0% | |
| CACATCAGTGGAGGAGGATT+TGG | + | chr3.1:5066084-5066103 | MS.gene56279:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.1:5065693-5065712 | MS.gene56279:CDS | 50.0% | |
| GAGCAACCAAGCTCCTTCAT+AGG | + | chr3.1:5066334-5066353 | MS.gene56279:CDS | 50.0% | |
| TGACCACCTACTCCAACACT+TGG | - | chr3.1:5066066-5066085 | None:intergenic | 50.0% | |
| TTGCCACCTATGAAGGAGCT+TGG | - | chr3.1:5066343-5066362 | None:intergenic | 50.0% | |
| ! | TGGAGGAGGATTTGGTACCT+TGG | + | chr3.1:5066092-5066111 | MS.gene56279:CDS | 50.0% |
| !! | TGTCCAAGTGTTGGAGTAGG+TGG | + | chr3.1:5066060-5066079 | MS.gene56279:intron | 50.0% |
| GGAGTAGGTGGTCACATCAG+TGG | + | chr3.1:5066072-5066091 | MS.gene56279:intron | 55.0% | |
| GTAGGTGGTCACATCAGTGG+AGG | + | chr3.1:5066075-5066094 | MS.gene56279:intron | 55.0% | |
| GGTGGTCACATCAGTGGAGG+AGG | + | chr3.1:5066078-5066097 | MS.gene56279:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5065282 | 5067122 | 5065282 | ID=MS.gene56279 |
| chr3.1 | mRNA | 5065282 | 5067122 | 5065282 | ID=MS.gene56279.t1;Parent=MS.gene56279 |
| chr3.1 | exon | 5065282 | 5065780 | 5065282 | ID=MS.gene56279.t1.exon1;Parent=MS.gene56279.t1 |
| chr3.1 | CDS | 5065282 | 5065780 | 5065282 | ID=cds.MS.gene56279.t1;Parent=MS.gene56279.t1 |
| chr3.1 | exon | 5066079 | 5066972 | 5066079 | ID=MS.gene56279.t1.exon2;Parent=MS.gene56279.t1 |
| chr3.1 | CDS | 5066079 | 5066972 | 5066079 | ID=cds.MS.gene56279.t1;Parent=MS.gene56279.t1 |
| chr3.1 | exon | 5067097 | 5067122 | 5067097 | ID=MS.gene56279.t1.exon3;Parent=MS.gene56279.t1 |
| chr3.1 | CDS | 5067097 | 5067122 | 5067097 | ID=cds.MS.gene56279.t1;Parent=MS.gene56279.t1 |
| Gene Sequence |
| Protein sequence |
