Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56282.t1 | AES68535.2 | 89.8 | 531 | 28 | 1 | 1 | 505 | 1 | 531 | 4.10E-272 | 946.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56282.t1 | Q9SUC6 | 51.7 | 524 | 211 | 8 | 8 | 494 | 11 | 529 | 9.8e-150 | 531.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56282.t1 | G7J0D0 | 89.8 | 531 | 28 | 1 | 1 | 505 | 1 | 531 | 2.9e-272 | 946.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028369 | MS.gene56282 | PPI |
| MS.gene35083 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene028346 | PPI |
| MS.gene35106 | MS.gene56282 | PPI |
| MS.gene28727 | MS.gene56282 | PPI |
| MS.gene35104 | MS.gene56282 | PPI |
| MS.gene35105 | MS.gene56282 | PPI |
| MS.gene36438 | MS.gene56282 | PPI |
| MS.gene003357 | MS.gene56282 | PPI |
| MS.gene28724 | MS.gene56282 | PPI |
| MS.gene028347 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene028353 | PPI |
| MS.gene02146 | MS.gene56282 | PPI |
| MS.gene028357 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene047977 | PPI |
| MS.gene56282 | MS.gene36444 | PPI |
| MS.gene35113 | MS.gene56282 | PPI |
| MS.gene36421 | MS.gene56282 | PPI |
| MS.gene28726 | MS.gene56282 | PPI |
| MS.gene28717 | MS.gene56282 | PPI |
| MS.gene28723 | MS.gene56282 | PPI |
| MS.gene35114 | MS.gene56282 | PPI |
| MS.gene028352 | MS.gene56282 | PPI |
| MS.gene35102 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene36435 | PPI |
| MS.gene56282 | MS.gene028354 | PPI |
| MS.gene28742 | MS.gene56282 | PPI |
| MS.gene36445 | MS.gene56282 | PPI |
| MS.gene28725 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene35103 | PPI |
| MS.gene36439 | MS.gene56282 | PPI |
| MS.gene56282 | MS.gene36437 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56282.t1 | MTR_3g009880 | 89.454 | 531 | 30 | 2 | 1 | 505 | 1 | 531 | 0.0 | 957 |
| MS.gene56282.t1 | MTR_3g009760 | 81.453 | 523 | 69 | 3 | 1 | 495 | 1 | 523 | 0.0 | 867 |
| MS.gene56282.t1 | MTR_3g009820 | 80.497 | 523 | 74 | 3 | 1 | 495 | 1 | 523 | 0.0 | 861 |
| MS.gene56282.t1 | MTR_4g094492 | 51.992 | 527 | 215 | 10 | 4 | 494 | 12 | 536 | 0.0 | 521 |
| MS.gene56282.t1 | MTR_4g094488 | 51.887 | 530 | 208 | 11 | 4 | 492 | 12 | 535 | 0.0 | 521 |
| MS.gene56282.t1 | MTR_2g031420 | 51.434 | 523 | 218 | 8 | 4 | 495 | 11 | 528 | 0.0 | 519 |
| MS.gene56282.t1 | MTR_2g031460 | 51.060 | 519 | 218 | 8 | 4 | 491 | 11 | 524 | 1.95e-180 | 517 |
| MS.gene56282.t1 | MTR_4g094495 | 52.000 | 525 | 214 | 9 | 5 | 492 | 15 | 538 | 2.41e-180 | 517 |
| MS.gene56282.t1 | MTR_4g094478 | 51.521 | 526 | 218 | 9 | 4 | 494 | 12 | 535 | 8.43e-180 | 516 |
| MS.gene56282.t1 | MTR_2g031390 | 49.318 | 513 | 225 | 6 | 19 | 498 | 23 | 533 | 6.50e-178 | 511 |
| MS.gene56282.t1 | MTR_4g094518 | 50.984 | 508 | 212 | 8 | 23 | 496 | 28 | 532 | 7.32e-172 | 495 |
| MS.gene56282.t1 | MTR_2g031560 | 47.665 | 514 | 231 | 8 | 18 | 497 | 17 | 526 | 5.65e-171 | 494 |
| MS.gene56282.t1 | MTR_4g094520 | 48.411 | 535 | 227 | 11 | 1 | 496 | 10 | 534 | 1.01e-170 | 493 |
| MS.gene56282.t1 | MTR_4g094480 | 48.106 | 528 | 235 | 9 | 3 | 492 | 7 | 533 | 8.76e-170 | 490 |
| MS.gene56282.t1 | MTR_4g094418 | 54.128 | 436 | 164 | 5 | 96 | 495 | 1 | 436 | 5.75e-167 | 486 |
| MS.gene56282.t1 | MTR_2g031590 | 45.920 | 527 | 237 | 9 | 9 | 494 | 13 | 532 | 7.19e-167 | 483 |
| MS.gene56282.t1 | MTR_4g094498 | 46.198 | 526 | 242 | 7 | 7 | 494 | 12 | 534 | 1.62e-165 | 480 |
| MS.gene56282.t1 | MTR_2g031490 | 42.561 | 531 | 250 | 9 | 8 | 494 | 19 | 538 | 2.05e-147 | 433 |
| MS.gene56282.t1 | MTR_4g091200 | 44.253 | 522 | 253 | 8 | 3 | 492 | 9 | 524 | 2.34e-147 | 432 |
| MS.gene56282.t1 | MTR_2g031510 | 42.969 | 512 | 252 | 9 | 19 | 494 | 33 | 540 | 1.03e-146 | 431 |
| MS.gene56282.t1 | MTR_4g057990 | 42.994 | 521 | 264 | 7 | 1 | 492 | 4 | 520 | 2.88e-146 | 429 |
| MS.gene56282.t1 | MTR_3g009850 | 88.889 | 225 | 25 | 0 | 271 | 495 | 5 | 229 | 1.10e-145 | 417 |
| MS.gene56282.t1 | MTR_4g091190 | 43.429 | 525 | 255 | 10 | 3 | 492 | 9 | 526 | 2.73e-144 | 425 |
| MS.gene56282.t1 | MTR_4g088355 | 44.862 | 506 | 237 | 8 | 24 | 492 | 31 | 531 | 5.94e-144 | 424 |
| MS.gene56282.t1 | MTR_6g017205 | 43.269 | 520 | 261 | 8 | 5 | 492 | 7 | 524 | 5.13e-143 | 422 |
| MS.gene56282.t1 | MTR_4g094515 | 42.911 | 529 | 263 | 8 | 3 | 494 | 624 | 1150 | 1.10e-140 | 434 |
| MS.gene56282.t1 | MTR_4g094515 | 42.278 | 518 | 259 | 10 | 12 | 492 | 24 | 538 | 1.58e-132 | 412 |
| MS.gene56282.t1 | MTR_4g091150 | 45.365 | 507 | 238 | 12 | 24 | 494 | 37 | 540 | 1.80e-139 | 413 |
| MS.gene56282.t1 | MTR_4g091170 | 45.133 | 452 | 200 | 6 | 71 | 492 | 107 | 540 | 9.92e-134 | 399 |
| MS.gene56282.t1 | MTR_2g023590 | 43.764 | 457 | 221 | 8 | 68 | 492 | 12 | 464 | 5.16e-129 | 384 |
| MS.gene56282.t1 | MTR_1g076830 | 39.451 | 474 | 252 | 8 | 52 | 491 | 60 | 532 | 2.20e-113 | 346 |
| MS.gene56282.t1 | MTR_2g031600 | 55.696 | 158 | 67 | 2 | 338 | 494 | 245 | 400 | 4.77e-56 | 192 |
| MS.gene56282.t1 | MTR_2g031600 | 40.260 | 231 | 111 | 3 | 9 | 212 | 14 | 244 | 9.19e-53 | 184 |
| MS.gene56282.t1 | MTR_2g084370 | 89.888 | 89 | 5 | 1 | 120 | 204 | 1 | 89 | 7.08e-50 | 166 |
| MS.gene56282.t1 | MTR_2g084405 | 61.250 | 80 | 5 | 2 | 120 | 173 | 1 | 80 | 1.00e-17 | 79.3 |
| MS.gene56282.t1 | MTR_8g062280 | 53.846 | 52 | 24 | 0 | 440 | 491 | 21 | 72 | 4.51e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56282.t1 | AT4G20860 | 50.386 | 518 | 215 | 9 | 14 | 494 | 17 | 529 | 1.28e-178 | 512 |
| MS.gene56282.t1 | AT5G44360 | 50.784 | 510 | 208 | 12 | 23 | 496 | 30 | 532 | 1.14e-173 | 500 |
| MS.gene56282.t1 | AT5G44360 | 50.784 | 510 | 208 | 12 | 23 | 496 | 46 | 548 | 2.28e-173 | 499 |
| MS.gene56282.t1 | AT5G44400 | 49.336 | 527 | 224 | 12 | 4 | 492 | 13 | 534 | 5.03e-170 | 491 |
| MS.gene56282.t1 | AT2G34790 | 47.070 | 529 | 228 | 10 | 5 | 492 | 11 | 528 | 1.73e-164 | 476 |
| MS.gene56282.t1 | AT1G30760 | 47.835 | 508 | 224 | 10 | 20 | 492 | 47 | 548 | 1.13e-163 | 475 |
| MS.gene56282.t1 | AT1G30760 | 47.835 | 508 | 224 | 10 | 20 | 492 | 29 | 530 | 1.23e-163 | 474 |
| MS.gene56282.t1 | AT5G44390 | 47.529 | 526 | 235 | 12 | 9 | 496 | 20 | 542 | 1.61e-160 | 467 |
| MS.gene56282.t1 | AT4G20820 | 44.190 | 525 | 244 | 10 | 8 | 497 | 19 | 529 | 6.20e-152 | 444 |
| MS.gene56282.t1 | AT5G44440 | 43.126 | 531 | 264 | 10 | 3 | 498 | 6 | 533 | 4.38e-151 | 442 |
| MS.gene56282.t1 | AT1G26420 | 44.382 | 534 | 244 | 12 | 5 | 497 | 7 | 528 | 8.97e-150 | 439 |
| MS.gene56282.t1 | AT5G44440 | 42.938 | 531 | 265 | 10 | 3 | 498 | 76 | 603 | 9.35e-150 | 441 |
| MS.gene56282.t1 | AT5G44380 | 46.212 | 528 | 241 | 13 | 5 | 492 | 13 | 537 | 3.11e-149 | 438 |
| MS.gene56282.t1 | AT5G44380 | 46.212 | 528 | 241 | 13 | 5 | 492 | 53 | 577 | 6.06e-149 | 439 |
| MS.gene56282.t1 | AT1G30710 | 42.395 | 526 | 263 | 10 | 4 | 495 | 10 | 529 | 3.39e-146 | 430 |
| MS.gene56282.t1 | AT1G26390 | 43.960 | 505 | 247 | 9 | 26 | 497 | 29 | 530 | 2.66e-145 | 427 |
| MS.gene56282.t1 | AT5G44410 | 43.178 | 535 | 256 | 12 | 1 | 497 | 8 | 532 | 7.77e-145 | 426 |
| MS.gene56282.t1 | AT1G26380 | 43.595 | 523 | 256 | 10 | 6 | 494 | 8 | 525 | 8.48e-145 | 426 |
| MS.gene56282.t1 | AT1G34575 | 42.529 | 522 | 259 | 11 | 4 | 493 | 11 | 523 | 6.98e-144 | 424 |
| MS.gene56282.t1 | AT1G26410 | 43.141 | 503 | 250 | 9 | 26 | 496 | 53 | 551 | 2.18e-142 | 421 |
| MS.gene56282.t1 | AT1G30700 | 44.213 | 527 | 252 | 10 | 4 | 494 | 6 | 526 | 1.51e-140 | 415 |
| MS.gene56282.t1 | AT4G20840 | 41.071 | 504 | 260 | 9 | 24 | 494 | 36 | 535 | 7.97e-139 | 411 |
| MS.gene56282.t1 | AT1G01980 | 41.667 | 516 | 263 | 9 | 15 | 494 | 21 | 534 | 1.65e-137 | 408 |
| MS.gene56282.t1 | AT1G26400 | 42.315 | 527 | 256 | 11 | 3 | 491 | 5 | 521 | 7.39e-137 | 406 |
| MS.gene56282.t1 | AT1G11770 | 40.678 | 531 | 276 | 10 | 1 | 494 | 1 | 529 | 6.34e-135 | 401 |
| MS.gene56282.t1 | AT4G20800 | 41.800 | 500 | 257 | 8 | 24 | 492 | 27 | 523 | 2.77e-133 | 397 |
| MS.gene56282.t1 | AT4G20830 | 41.339 | 508 | 263 | 9 | 19 | 494 | 32 | 536 | 5.70e-133 | 396 |
| MS.gene56282.t1 | AT4G20830 | 41.339 | 508 | 263 | 9 | 19 | 494 | 32 | 536 | 2.09e-132 | 396 |
| MS.gene56282.t1 | AT1G30730 | 40.525 | 533 | 268 | 11 | 1 | 493 | 1 | 524 | 2.10e-132 | 394 |
| MS.gene56282.t1 | AT1G30720 | 39.766 | 513 | 274 | 10 | 13 | 493 | 16 | 525 | 4.01e-127 | 381 |
| MS.gene56282.t1 | AT1G30740 | 40.076 | 529 | 278 | 9 | 3 | 494 | 2 | 528 | 6.31e-125 | 375 |
| MS.gene56282.t1 | AT1G30740 | 40.076 | 529 | 278 | 9 | 3 | 494 | 5 | 531 | 9.46e-125 | 375 |
| MS.gene56282.t1 | AT2G34810 | 39.265 | 517 | 276 | 10 | 15 | 494 | 24 | 539 | 3.40e-124 | 374 |
Find 93 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGCATCAAGTACTTTA+AGG | 0.182488 | 3.1:+5122359 | MS.gene56282:CDS |
| AAGCTGGTTTAGAAGGTATT+TGG | 0.185112 | 3.1:+5122025 | MS.gene56282:CDS |
| AATGAGAGTTTCCCTGAATT+AGG | 0.214946 | 3.1:+5121841 | MS.gene56282:CDS |
| CACATTAGCATTACTTATAA+TGG | 0.248787 | 3.1:+5122068 | MS.gene56282:CDS |
| TTGTCTTGCAACCCTAATTC+AGG | 0.255332 | 3.1:-5121853 | None:intergenic |
| GTTCACCATTCATAAAATTC+TGG | 0.274997 | 3.1:+5121648 | MS.gene56282:CDS |
| GGAGGTAGTGCTGCTAGTTT+TGG | 0.287867 | 3.1:+5121571 | MS.gene56282:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.1:-5120895 | None:intergenic |
| CATCCACTTTATATGTTTGT+TGG | 0.301040 | 3.1:-5122211 | None:intergenic |
| TCCTTCCATTTACATCTATA+AGG | 0.320941 | 3.1:-5121501 | None:intergenic |
| CTTGATGAGTTCACCCATCT+TGG | 0.322731 | 3.1:-5120845 | None:intergenic |
| GTGGATTTGGTACCTTGTTT+AGG | 0.323328 | 3.1:+5121440 | MS.gene56282:CDS |
| TAGAGGTGGTGGAATTAATT+AGG | 0.323700 | 3.1:+5122485 | MS.gene56282:CDS |
| GGGCTGCATATTACAACTAT+AGG | 0.325111 | 3.1:+5122280 | MS.gene56282:CDS |
| GCTTACCTTATAGATGTAAA+TGG | 0.326848 | 3.1:+5121496 | MS.gene56282:CDS |
| AATCTCTTTGCTGCATAGTA+TGG | 0.336993 | 3.1:-5120918 | None:intergenic |
| CACATCAGTGGAGGTGGATT+TGG | 0.356454 | 3.1:+5121427 | MS.gene56282:CDS |
| ATGAGAGTTTCCCTGAATTA+GGG | 0.357011 | 3.1:+5121842 | MS.gene56282:CDS |
| CTGTTAAGCAAGAGTTCTAT+AGG | 0.363323 | 3.1:-5121940 | None:intergenic |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.1:-5122015 | None:intergenic |
| GCTACTCAGAAGCAAGTGTT+TGG | 0.368108 | 3.1:+5122340 | MS.gene56282:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.1:+5121076 | MS.gene56282:CDS |
| AGCAGCACTACCTCCTCTTA+TGG | 0.383413 | 3.1:-5121563 | None:intergenic |
| AAATGAATCTGCTTGGATTC+AGG | 0.391403 | 3.1:+5121060 | MS.gene56282:CDS |
| TAGTCAGATTTGGCTTTGAA+TGG | 0.394226 | 3.1:-5121982 | None:intergenic |
| TCACTCATTCTTCCACCATA+TGG | 0.398978 | 3.1:-5122093 | None:intergenic |
| AGAATAGTTGCTCAAACTAA+TGG | 0.401600 | 3.1:+5121742 | MS.gene56282:CDS |
| TGGGGCATCAAGTACTTTAA+GGG | 0.402731 | 3.1:+5122360 | MS.gene56282:CDS |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.1:-5122021 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.1:+5122018 | MS.gene56282:CDS |
| CTACTCAGAAGCAAGTGTTT+GGG | 0.418460 | 3.1:+5122341 | MS.gene56282:CDS |
| GGCTGCATATTACAACTATA+GGG | 0.426482 | 3.1:+5122281 | MS.gene56282:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.427868 | 3.1:-5122258 | None:intergenic |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.1:-5121008 | None:intergenic |
| ATGTTTAATTCTCTCTTCCT+TGG | 0.438442 | 3.1:+5121790 | MS.gene56282:CDS |
| ATACTATGCAGCAAAGAGAT+TGG | 0.444376 | 3.1:+5120920 | MS.gene56282:CDS |
| CCACAAAACTCATTCATAGA+TGG | 0.453753 | 3.1:+5121680 | MS.gene56282:CDS |
| TCATTCTTGCATGGAAGATA+AGG | 0.459271 | 3.1:+5121596 | MS.gene56282:CDS |
| ATCTTGCAAATGAATCTGCT+TGG | 0.463262 | 3.1:+5121053 | MS.gene56282:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.467290 | 3.1:+5122294 | MS.gene56282:CDS |
| GATTCTTAATAGAAAATCAA+TGG | 0.470027 | 3.1:+5121522 | MS.gene56282:CDS |
| TAAGGGAAACTTTAAGCGAT+TGG | 0.470910 | 3.1:+5122377 | MS.gene56282:CDS |
| TTCAATGATTCCAATACTTG+TGG | 0.473264 | 3.1:-5120815 | None:intergenic |
| TGTCTTGCAACCCTAATTCA+GGG | 0.475992 | 3.1:-5121852 | None:intergenic |
| ACCCTTGTATCCACAAGTAT+TGG | 0.476913 | 3.1:+5120805 | MS.gene56282:CDS |
| AAGACTGCATTGAAATGAGT+TGG | 0.478707 | 3.1:+5121875 | MS.gene56282:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.1:-5121007 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.1:-5120970 | None:intergenic |
| AGTCAGATTTGGCTTTGAAT+GGG | 0.479319 | 3.1:-5121981 | None:intergenic |
| TTCCAATACTTGTGGATACA+AGG | 0.480417 | 3.1:-5120807 | None:intergenic |
| CATTGAAACAAAATCCAAGA+TGG | 0.486669 | 3.1:+5120831 | MS.gene56282:CDS |
| AGTCCAACAAACATATAAAG+TGG | 0.489218 | 3.1:+5122208 | MS.gene56282:CDS |
| ATTCATAAAATTCTGGAAGA+AGG | 0.493097 | 3.1:+5121655 | MS.gene56282:CDS |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.1:-5120894 | None:intergenic |
| CTTGTACAACATACAATACA+TGG | 0.505452 | 3.1:+5122158 | MS.gene56282:CDS |
| TGCTTCTTCAACTTCAGTTG+AGG | 0.505760 | 3.1:+5120706 | MS.gene56282:CDS |
| GGCGGTCACATCAGTGGAGG+TGG | 0.518000 | 3.1:+5121421 | MS.gene56282:intron |
| ATTCTTAATAGAAAATCAAT+GGG | 0.522100 | 3.1:+5121523 | MS.gene56282:CDS |
| GGTACCTTGTTTAGGAAACA+TGG | 0.537180 | 3.1:+5121448 | MS.gene56282:CDS |
| TTACTTATAATGGAACCATA+TGG | 0.538421 | 3.1:+5122078 | MS.gene56282:CDS |
| GATTGGTTTACAGATGAGAG+TGG | 0.540857 | 3.1:+5120937 | MS.gene56282:CDS |
| TCTTGCATGGAAGATAAGGT+TGG | 0.542256 | 3.1:+5121600 | MS.gene56282:CDS |
| GTCATTACTCCTTCACATAG+AGG | 0.545004 | 3.1:+5122468 | MS.gene56282:CDS |
| TTACAGATGAGAGTGGTTAG+TGG | 0.549198 | 3.1:+5120944 | MS.gene56282:CDS |
| ATGGTGAACACTGTAACAAT+AGG | 0.551113 | 3.1:-5121634 | None:intergenic |
| ATTGAAACAAAATCCAAGAT+GGG | 0.551532 | 3.1:+5120832 | MS.gene56282:CDS |
| CAAGATTAATGTCAATCGAA+CGG | 0.553223 | 3.1:-5121036 | None:intergenic |
| TAGTTGTAATATGCAGCCCT+TGG | 0.555414 | 3.1:-5122276 | None:intergenic |
| ACTCCTTCACATAGAGGTGG+TGG | 0.555530 | 3.1:+5122474 | MS.gene56282:CDS |
| CTTATGTTTCAAAGTCTCCA+AGG | 0.559379 | 3.1:+5122259 | MS.gene56282:CDS |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.1:+5122009 | MS.gene56282:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.1:-5120890 | None:intergenic |
| ACATACAATACATGGTCAAG+TGG | 0.580747 | 3.1:+5122166 | MS.gene56282:CDS |
| TTATGTTTCAAAGTCTCCAA+GGG | 0.584994 | 3.1:+5122260 | MS.gene56282:CDS |
| ATAGGGATCTTGATATAGGA+AGG | 0.589814 | 3.1:+5122298 | MS.gene56282:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.1:+5120962 | MS.gene56282:CDS |
| ACCTTATAGATGTAAATGGA+AGG | 0.593956 | 3.1:+5121500 | MS.gene56282:CDS |
| GAATCTGCTTGGATTCAGGC+TGG | 0.594184 | 3.1:+5121064 | MS.gene56282:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.597018 | 3.1:+5122342 | MS.gene56282:CDS |
| GTGAACACTGTAACAATAGG+TGG | 0.602884 | 3.1:-5121631 | None:intergenic |
| GGAATAGGCGGTCACATCAG+TGG | 0.603755 | 3.1:+5121415 | MS.gene56282:intron |
| GGAGTAATGACATTTAGTAG+AGG | 0.607260 | 3.1:-5122456 | None:intergenic |
| TCCAATACTTGTGGATACAA+GGG | 0.610964 | 3.1:-5120806 | None:intergenic |
| CTTATAATGGAACCATATGG+TGG | 0.625352 | 3.1:+5122081 | MS.gene56282:CDS |
| CATACAATACATGGTCAAGT+GGG | 0.625707 | 3.1:+5122167 | MS.gene56282:CDS |
| ATTCCACCACCTCTATGTGA+AGG | 0.628617 | 3.1:-5122477 | None:intergenic |
| CAGATGAGAGTGGTTAGTGG+TGG | 0.632301 | 3.1:+5120947 | MS.gene56282:CDS |
| GAAGGTGTCATAATGAGAAG+AGG | 0.638440 | 3.1:-5120872 | None:intergenic |
| TAGACCATGTTTCCTAAACA+AGG | 0.647398 | 3.1:-5121452 | None:intergenic |
| TAAGGTAAGCATCAACAACA+TGG | 0.652825 | 3.1:-5121483 | None:intergenic |
| ATAGGCGGTCACATCAGTGG+AGG | 0.689151 | 3.1:+5121418 | MS.gene56282:intron |
| ATTACTCCTTCACATAGAGG+TGG | 0.692038 | 3.1:+5122471 | MS.gene56282:CDS |
| AAGGAAGAGAGAATTAAACA+TGG | 0.696240 | 3.1:-5121788 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAATAGAAAATCAAT+GGG | + | chr3.1:5121523-5121542 | MS.gene56282:CDS | 15.0% |
| !! | GATTCTTAATAGAAAATCAA+TGG | + | chr3.1:5121522-5121541 | MS.gene56282:CDS | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.1:5121010-5121029 | None:intergenic | 25.0% |
| ! | ATTGAAACAAAATCCAAGAT+GGG | + | chr3.1:5120832-5120851 | MS.gene56282:CDS | 25.0% |
| ! | CACATTAGCATTACTTATAA+TGG | + | chr3.1:5122068-5122087 | MS.gene56282:CDS | 25.0% |
| !! | ATTCATAAAATTCTGGAAGA+AGG | + | chr3.1:5121655-5121674 | MS.gene56282:CDS | 25.0% |
| !! | TTAAGTTTTCTTTTCTTCCA+AGG | - | chr3.1:5121810-5121829 | None:intergenic | 25.0% |
| !! | TTACTTATAATGGAACCATA+TGG | + | chr3.1:5122078-5122097 | MS.gene56282:CDS | 25.0% |
| !! | TTCTTCCAGAATTTTATGAA+TGG | - | chr3.1:5121656-5121675 | None:intergenic | 25.0% |
| !!! | ATTAAGTTGTTTTTCCAAAC+AGG | + | chr3.1:5121376-5121395 | MS.gene56282:intron | 25.0% |
| !!! | TTAAGTTGTTTTTCCAAACA+GGG | + | chr3.1:5121377-5121396 | MS.gene56282:intron | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.1:5122294-5122313 | MS.gene56282:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.1:5120973-5120992 | None:intergenic | 30.0% | |
| AAGGAAGAGAGAATTAAACA+TGG | - | chr3.1:5121791-5121810 | None:intergenic | 30.0% | |
| ACCTTATAGATGTAAATGGA+AGG | + | chr3.1:5121500-5121519 | MS.gene56282:CDS | 30.0% | |
| AGAATAGTTGCTCAAACTAA+TGG | + | chr3.1:5121742-5121761 | MS.gene56282:CDS | 30.0% | |
| AGCACAAGCAAATTATAAGA+CGG | + | chr3.1:5121185-5121204 | MS.gene56282:intron | 30.0% | |
| AGTCCAACAAACATATAAAG+TGG | + | chr3.1:5122208-5122227 | MS.gene56282:CDS | 30.0% | |
| ATGTTTAATTCTCTCTTCCT+TGG | + | chr3.1:5121790-5121809 | MS.gene56282:CDS | 30.0% | |
| CAAGATTAATGTCAATCGAA+CGG | - | chr3.1:5121039-5121058 | None:intergenic | 30.0% | |
| CATCCACTTTATATGTTTGT+TGG | - | chr3.1:5122214-5122233 | None:intergenic | 30.0% | |
| CATTGAAACAAAATCCAAGA+TGG | + | chr3.1:5120831-5120850 | MS.gene56282:CDS | 30.0% | |
| CTTGTACAACATACAATACA+TGG | + | chr3.1:5122158-5122177 | MS.gene56282:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.1:5121011-5121030 | None:intergenic | 30.0% | |
| GCTTACCTTATAGATGTAAA+TGG | + | chr3.1:5121496-5121515 | MS.gene56282:CDS | 30.0% | |
| GTTCACCATTCATAAAATTC+TGG | + | chr3.1:5121648-5121667 | MS.gene56282:CDS | 30.0% | |
| TCCTTCCATTTACATCTATA+AGG | - | chr3.1:5121504-5121523 | None:intergenic | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.1:5120897-5120916 | None:intergenic | 30.0% | |
| TTATGTTTCAAAGTCTCCAA+GGG | + | chr3.1:5122260-5122279 | MS.gene56282:CDS | 30.0% | |
| TTCAATGATTCCAATACTTG+TGG | - | chr3.1:5120818-5120837 | None:intergenic | 30.0% | |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.1:5122427-5122446 | None:intergenic | 30.0% |
| ! | CTCTTTTACATAGTCAGATT+TGG | - | chr3.1:5121995-5122014 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.1:5122426-5122445 | None:intergenic | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+AGG | + | chr3.1:5121901-5121920 | MS.gene56282:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.1:5121540-5121559 | MS.gene56282:CDS | 30.0% |
| !!! | GATAAACACTGTTTTTTGAG+TGG | - | chr3.1:5120780-5120799 | None:intergenic | 30.0% |
| AAATGAATCTGCTTGGATTC+AGG | + | chr3.1:5121060-5121079 | MS.gene56282:CDS | 35.0% | |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.1:5121875-5121894 | MS.gene56282:CDS | 35.0% | |
| AAGTCAAACTAATTCACCTG+AGG | - | chr3.1:5121149-5121168 | None:intergenic | 35.0% | |
| AATCTCTTTGCTGCATAGTA+TGG | - | chr3.1:5120921-5120940 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCCTGAATT+AGG | + | chr3.1:5121841-5121860 | MS.gene56282:CDS | 35.0% | |
| ACATACAATACATGGTCAAG+TGG | + | chr3.1:5122166-5122185 | MS.gene56282:CDS | 35.0% | |
| AGTCAAACTAATTCACCTGA+GGG | - | chr3.1:5121148-5121167 | None:intergenic | 35.0% | |
| ATACTATGCAGCAAAGAGAT+TGG | + | chr3.1:5120920-5120939 | MS.gene56282:CDS | 35.0% | |
| ATAGGGATCTTGATATAGGA+AGG | + | chr3.1:5122298-5122317 | MS.gene56282:CDS | 35.0% | |
| ATCTTGCAAATGAATCTGCT+TGG | + | chr3.1:5121053-5121072 | MS.gene56282:CDS | 35.0% | |
| ATGAGAGTTTCCCTGAATTA+GGG | + | chr3.1:5121842-5121861 | MS.gene56282:CDS | 35.0% | |
| ATGGTGAACACTGTAACAAT+AGG | - | chr3.1:5121637-5121656 | None:intergenic | 35.0% | |
| CATACAATACATGGTCAAGT+GGG | + | chr3.1:5122167-5122186 | MS.gene56282:CDS | 35.0% | |
| CCACAAAACTCATTCATAGA+TGG | + | chr3.1:5121680-5121699 | MS.gene56282:CDS | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.1:5120898-5120917 | None:intergenic | 35.0% | |
| CTGTTAAGCAAGAGTTCTAT+AGG | - | chr3.1:5121943-5121962 | None:intergenic | 35.0% | |
| CTTATGTTTCAAAGTCTCCA+AGG | + | chr3.1:5122259-5122278 | MS.gene56282:CDS | 35.0% | |
| GGAGTAATGACATTTAGTAG+AGG | - | chr3.1:5122459-5122478 | None:intergenic | 35.0% | |
| GGCTGCATATTACAACTATA+GGG | + | chr3.1:5122281-5122300 | MS.gene56282:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGAT+TGG | + | chr3.1:5122377-5122396 | MS.gene56282:CDS | 35.0% | |
| TAAGGTAAGCATCAACAACA+TGG | - | chr3.1:5121486-5121505 | None:intergenic | 35.0% | |
| TAGACCATGTTTCCTAAACA+AGG | - | chr3.1:5121455-5121474 | None:intergenic | 35.0% | |
| TCATTCTTGCATGGAAGATA+AGG | + | chr3.1:5121596-5121615 | MS.gene56282:CDS | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.1:5121118-5121137 | MS.gene56282:intron | 35.0% | |
| TCCAATACTTGTGGATACAA+GGG | - | chr3.1:5120809-5120828 | None:intergenic | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.1:5121122-5121141 | None:intergenic | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.1:5120893-5120912 | None:intergenic | 35.0% | |
| TTCCAATACTTGTGGATACA+AGG | - | chr3.1:5120810-5120829 | None:intergenic | 35.0% | |
| ! | CCATCTATGAATGAGTTTTG+TGG | - | chr3.1:5121683-5121702 | None:intergenic | 35.0% |
| ! | CTTATAATGGAACCATATGG+TGG | + | chr3.1:5122081-5122100 | MS.gene56282:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.1:5122428-5122447 | None:intergenic | 35.0% |
| ! | TACAAGTTTCCTTTTCTGTG+TGG | - | chr3.1:5122144-5122163 | None:intergenic | 35.0% |
| ! | TTCCTTTTCTGTGTGGAAAA+GGG | - | chr3.1:5122137-5122156 | None:intergenic | 35.0% |
| ! | TTTCCTTTTCTGTGTGGAAA+AGG | - | chr3.1:5122138-5122157 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.1:5122025-5122044 | MS.gene56282:CDS | 35.0% |
| !! | AGTCAGATTTGGCTTTGAAT+GGG | - | chr3.1:5121984-5122003 | None:intergenic | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.1:5122261-5122280 | None:intergenic | 35.0% |
| !! | TAGTCAGATTTGGCTTTGAA+TGG | - | chr3.1:5121985-5122004 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.1:5122418-5122437 | MS.gene56282:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.1:5121539-5121558 | MS.gene56282:CDS | 35.0% |
| ACCCTTGTATCCACAAGTAT+TGG | + | chr3.1:5120805-5120824 | MS.gene56282:CDS | 40.0% | |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.1:5122024-5122043 | None:intergenic | 40.0% | |
| ATTACTCCTTCACATAGAGG+TGG | + | chr3.1:5122471-5122490 | MS.gene56282:CDS | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.1:5122341-5122360 | MS.gene56282:CDS | 40.0% | |
| GGGCTGCATATTACAACTAT+AGG | + | chr3.1:5122280-5122299 | MS.gene56282:CDS | 40.0% | |
| GTCAAACTAATTCACCTGAG+GGG | - | chr3.1:5121147-5121166 | None:intergenic | 40.0% | |
| GTCATTACTCCTTCACATAG+AGG | + | chr3.1:5122468-5122487 | MS.gene56282:CDS | 40.0% | |
| GTGAACACTGTAACAATAGG+TGG | - | chr3.1:5121634-5121653 | None:intergenic | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.1:5122342-5122361 | MS.gene56282:CDS | 40.0% | |
| TAGTTGTAATATGCAGCCCT+TGG | - | chr3.1:5122279-5122298 | None:intergenic | 40.0% | |
| TCACTCATTCTTCCACCATA+TGG | - | chr3.1:5122096-5122115 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.1:5122018-5122037 | None:intergenic | 40.0% | |
| TCTTGCATGGAAGATAAGGT+TGG | + | chr3.1:5121600-5121619 | MS.gene56282:CDS | 40.0% | |
| TGCTTCTTCAACTTCAGTTG+AGG | + | chr3.1:5120706-5120725 | MS.gene56282:CDS | 40.0% | |
| TGTCTTGCAACCCTAATTCA+GGG | - | chr3.1:5121855-5121874 | None:intergenic | 40.0% | |
| TTACAGATGAGAGTGGTTAG+TGG | + | chr3.1:5120944-5120963 | MS.gene56282:CDS | 40.0% | |
| TTGTCTTGCAACCCTAATTC+AGG | - | chr3.1:5121856-5121875 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.1:5122018-5122037 | MS.gene56282:CDS | 40.0% |
| ! | ATCCCTTTTCCACACAGAAA+AGG | + | chr3.1:5122132-5122151 | MS.gene56282:CDS | 40.0% |
| ! | GGTACCTTGTTTAGGAAACA+TGG | + | chr3.1:5121448-5121467 | MS.gene56282:CDS | 40.0% |
| ! | GTGGATTTGGTACCTTGTTT+AGG | + | chr3.1:5121440-5121459 | MS.gene56282:CDS | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.1:5122360-5122379 | MS.gene56282:CDS | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.1:5122359-5122378 | MS.gene56282:CDS | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.1:5120875-5120894 | None:intergenic | 40.0% |
| !! | GATTGGTTTACAGATGAGAG+TGG | + | chr3.1:5120937-5120956 | MS.gene56282:CDS | 40.0% |
| !!! | GATGTTTTTTGGGCCATAAG+AGG | + | chr3.1:5121550-5121569 | MS.gene56282:CDS | 40.0% |
| !!! | GTTTTGGAGTCATTCTTGCA+TGG | + | chr3.1:5121587-5121606 | MS.gene56282:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.1:5122009-5122028 | MS.gene56282:CDS | 45.0% | |
| AAAGTTCATGGATTCCCCTC+AGG | + | chr3.1:5121130-5121149 | MS.gene56282:intron | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.1:5120962-5120981 | MS.gene56282:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.1:5121076-5121095 | MS.gene56282:CDS | 45.0% | |
| ATTCCACCACCTCTATGTGA+AGG | - | chr3.1:5122480-5122499 | None:intergenic | 45.0% | |
| CTTGATGAGTTCACCCATCT+TGG | - | chr3.1:5120848-5120867 | None:intergenic | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.1:5122340-5122359 | MS.gene56282:CDS | 45.0% | |
| !!! | GGCTTTGAATGGGCTTTTGT+AGG | - | chr3.1:5121974-5121993 | None:intergenic | 45.0% |
| !!! | GTTTTTTGGGCCATAAGAGG+AGG | + | chr3.1:5121553-5121572 | MS.gene56282:CDS | 45.0% |
| ACTCCTTCACATAGAGGTGG+TGG | + | chr3.1:5122474-5122493 | MS.gene56282:CDS | 50.0% | |
| AGCAGCACTACCTCCTCTTA+TGG | - | chr3.1:5121566-5121585 | None:intergenic | 50.0% | |
| CAGATGAGAGTGGTTAGTGG+TGG | + | chr3.1:5120947-5120966 | MS.gene56282:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.1:5121064-5121083 | MS.gene56282:CDS | 50.0% | |
| ! | CACATCAGTGGAGGTGGATT+TGG | + | chr3.1:5121427-5121446 | MS.gene56282:CDS | 50.0% |
| !! | GGAGGTAGTGCTGCTAGTTT+TGG | + | chr3.1:5121571-5121590 | MS.gene56282:CDS | 50.0% |
| !! | TTGTGTCCGAGCGTTGGAAT+AGG | + | chr3.1:5121400-5121419 | MS.gene56282:intron | 50.0% |
| ATAGGCGGTCACATCAGTGG+AGG | + | chr3.1:5121418-5121437 | MS.gene56282:intron | 55.0% | |
| GGAATAGGCGGTCACATCAG+TGG | + | chr3.1:5121415-5121434 | MS.gene56282:intron | 55.0% | |
| TGACCGCCTATTCCAACGCT+CGG | - | chr3.1:5121409-5121428 | None:intergenic | 55.0% | |
| !! | TGTCCGAGCGTTGGAATAGG+CGG | + | chr3.1:5121403-5121422 | MS.gene56282:intron | 55.0% |
| ACAGGGTTGTGTCCGAGCGT+TGG | + | chr3.1:5121394-5121413 | MS.gene56282:intron | 60.0% | |
| CGCTCGGACACAACCCTGTT+TGG | - | chr3.1:5121393-5121412 | None:intergenic | 60.0% | |
| GGCGGTCACATCAGTGGAGG+TGG | + | chr3.1:5121421-5121440 | MS.gene56282:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5120659 | 5122506 | 5120659 | ID=MS.gene56282 |
| chr3.1 | mRNA | 5120659 | 5122506 | 5120659 | ID=MS.gene56282.t1;Parent=MS.gene56282 |
| chr3.1 | exon | 5120659 | 5121097 | 5120659 | ID=MS.gene56282.t1.exon1;Parent=MS.gene56282.t1 |
| chr3.1 | CDS | 5120659 | 5121097 | 5120659 | ID=cds.MS.gene56282.t1;Parent=MS.gene56282.t1 |
| chr3.1 | exon | 5121422 | 5122506 | 5121422 | ID=MS.gene56282.t1.exon2;Parent=MS.gene56282.t1 |
| chr3.1 | CDS | 5121422 | 5122506 | 5121422 | ID=cds.MS.gene56282.t1;Parent=MS.gene56282.t1 |
| Gene Sequence |
| Protein sequence |
