Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57663.t1 | XP_024636800.1 | 98.5 | 67 | 1 | 0 | 1 | 67 | 38 | 104 | 7.30E-28 | 132.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57663.t1 | P81748 | 43.1 | 65 | 37 | 0 | 1 | 65 | 37 | 101 | 4.2e-08 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57663.t1 | G7JUA1 | 98.5 | 67 | 1 | 0 | 1 | 67 | 776 | 842 | 5.3e-28 | 132.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene57663 | 0.821037 | 4.99E-53 | -1.69E-46 |
| MS.gene049173 | MS.gene57663 | 0.805137 | 1.54E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene57663 | 0.822449 | 2.35E-53 | -1.69E-46 |
| MS.gene049232 | MS.gene57663 | 0.816331 | 5.84E-52 | -1.69E-46 |
| MS.gene049285 | MS.gene57663 | 0.854576 | 1.12E-61 | -1.69E-46 |
| MS.gene049320 | MS.gene57663 | 0.85944 | 4.13E-63 | -1.69E-46 |
| MS.gene05010 | MS.gene57663 | 0.838966 | 2.10E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene57663 | 0.830417 | 2.97E-55 | -1.69E-46 |
| MS.gene050165 | MS.gene57663 | 0.839398 | 1.62E-57 | -1.69E-46 |
| MS.gene050333 | MS.gene57663 | 0.832317 | 1.01E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene57663 | 0.854542 | 1.15E-61 | -1.69E-46 |
| MS.gene050518 | MS.gene57663 | 0.858838 | 6.26E-63 | -1.69E-46 |
| MS.gene050612 | MS.gene57663 | 0.857343 | 1.74E-62 | -1.69E-46 |
| MS.gene050675 | MS.gene57663 | 0.802556 | 5.30E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene57663 | 0.814209 | 1.73E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene57663 | 0.824657 | 7.17E-54 | -1.69E-46 |
| MS.gene050986 | MS.gene57663 | 0.819506 | 1.12E-52 | -1.69E-46 |
| MS.gene05100 | MS.gene57663 | 0.815045 | 1.13E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene57663 | 0.815025 | 1.14E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene57663 | 0.842968 | 1.87E-58 | -1.69E-46 |
| MS.gene051949 | MS.gene57663 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene57663 | 0.801373 | 9.28E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene57663 | 0.808093 | 3.67E-50 | -1.69E-46 |
| MS.gene053104 | MS.gene57663 | 0.800282 | 1.55E-48 | -1.69E-46 |
| MS.gene053213 | MS.gene57663 | 0.829456 | 5.10E-55 | -1.69E-46 |
| MS.gene053286 | MS.gene57663 | 0.834498 | 2.90E-56 | -1.69E-46 |
| MS.gene053561 | MS.gene57663 | -0.807398 | 5.16E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene57663 | 0.80925 | 2.08E-50 | -1.69E-46 |
| MS.gene054374 | MS.gene57663 | 0.845404 | 4.15E-59 | -1.69E-46 |
| MS.gene054600 | MS.gene57663 | 0.809818 | 1.57E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene57663 | 0.868275 | 7.37E-66 | -1.69E-46 |
| MS.gene054954 | MS.gene57663 | 0.857239 | 1.87E-62 | -1.69E-46 |
| MS.gene055212 | MS.gene57663 | 0.816483 | 5.40E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene57663 | 0.842727 | 2.17E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene57663 | 0.845673 | 3.51E-59 | -1.69E-46 |
| MS.gene055577 | MS.gene57663 | 0.823792 | 1.14E-53 | -1.69E-46 |
| MS.gene055634 | MS.gene57663 | 0.812482 | 4.15E-51 | -1.69E-46 |
| MS.gene055638 | MS.gene57663 | 0.813269 | 2.79E-51 | -1.69E-46 |
| MS.gene055750 | MS.gene57663 | 0.802586 | 5.23E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene57663 | 0.814241 | 1.70E-51 | -1.69E-46 |
| MS.gene055776 | MS.gene57663 | 0.827056 | 1.93E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene57663 | 0.818905 | 1.54E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene57663 | 0.830465 | 2.89E-55 | -1.69E-46 |
| MS.gene056510 | MS.gene57663 | 0.823889 | 1.09E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene57663 | 0.857646 | 1.42E-62 | -1.69E-46 |
| MS.gene056692 | MS.gene57663 | 0.807683 | 4.48E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene57663 | 0.852311 | 5.02E-61 | -1.69E-46 |
| MS.gene058544 | MS.gene57663 | 0.800493 | 1.40E-48 | -1.69E-46 |
| MS.gene058723 | MS.gene57663 | 0.803663 | 3.13E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene57663 | 0.836311 | 1.01E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene57663 | 0.856229 | 3.70E-62 | -1.69E-46 |
| MS.gene059029 | MS.gene57663 | 0.820871 | 5.45E-53 | -1.69E-46 |
| MS.gene05913 | MS.gene57663 | 0.830971 | 2.17E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene57663 | 0.843404 | 1.43E-58 | -1.69E-46 |
| MS.gene059718 | MS.gene57663 | 0.818044 | 2.41E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene57663 | 0.844003 | 9.89E-59 | -1.69E-46 |
| MS.gene060337 | MS.gene57663 | -0.811457 | 6.94E-51 | -1.69E-46 |
| MS.gene060703 | MS.gene57663 | 0.871967 | 4.56E-67 | -1.69E-46 |
| MS.gene060762 | MS.gene57663 | 0.811118 | 8.21E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene57663 | 0.831006 | 2.13E-55 | -1.69E-46 |
| MS.gene060982 | MS.gene57663 | 0.818667 | 1.74E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57663.t1 | MTR_4g127370 | 98.507 | 67 | 1 | 0 | 1 | 67 | 776 | 842 | 6.18e-40 | 138 |
| MS.gene57663.t1 | MTR_4g127360 | 79.104 | 67 | 14 | 0 | 1 | 67 | 38 | 104 | 6.27e-33 | 109 |
| MS.gene57663.t1 | MTR_4g014040 | 65.672 | 67 | 23 | 0 | 1 | 67 | 38 | 104 | 1.57e-28 | 98.6 |
| MS.gene57663.t1 | MTR_4g014040 | 65.672 | 67 | 23 | 0 | 1 | 67 | 38 | 104 | 6.09e-28 | 97.1 |
| MS.gene57663.t1 | MTR_4g014040 | 65.672 | 67 | 23 | 0 | 1 | 67 | 38 | 104 | 1.34e-27 | 97.4 |
| MS.gene57663.t1 | MTR_7g059460 | 58.209 | 67 | 28 | 0 | 1 | 67 | 38 | 104 | 4.93e-23 | 84.3 |
| MS.gene57663.t1 | MTR_7g059460 | 58.209 | 67 | 28 | 0 | 1 | 67 | 38 | 104 | 1.23e-22 | 83.6 |
| MS.gene57663.t1 | MTR_7g070250 | 50.000 | 66 | 33 | 0 | 1 | 66 | 38 | 103 | 3.62e-19 | 74.7 |
| MS.gene57663.t1 | MTR_4g013730 | 48.485 | 66 | 34 | 0 | 1 | 66 | 38 | 103 | 1.14e-18 | 73.2 |
| MS.gene57663.t1 | MTR_0562s0020 | 48.485 | 66 | 34 | 0 | 1 | 66 | 38 | 103 | 1.14e-18 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57663.t1 | AT5G57170 | 87.879 | 66 | 8 | 0 | 1 | 66 | 38 | 103 | 6.95e-36 | 117 |
| MS.gene57663.t1 | AT5G57170 | 87.879 | 66 | 8 | 0 | 1 | 66 | 38 | 103 | 2.13e-35 | 115 |
| MS.gene57663.t1 | AT5G01650 | 62.687 | 67 | 25 | 0 | 1 | 67 | 38 | 104 | 1.29e-27 | 96.3 |
| MS.gene57663.t1 | AT5G01650 | 62.687 | 67 | 25 | 0 | 1 | 67 | 38 | 104 | 1.45e-27 | 96.3 |
| MS.gene57663.t1 | AT5G01650 | 62.687 | 67 | 25 | 0 | 1 | 67 | 38 | 104 | 1.45e-27 | 96.3 |
| MS.gene57663.t1 | AT5G01650 | 62.687 | 67 | 25 | 0 | 1 | 67 | 38 | 104 | 2.95e-27 | 95.1 |
| MS.gene57663.t1 | AT3G51660 | 43.750 | 64 | 36 | 0 | 2 | 65 | 39 | 102 | 5.84e-14 | 61.2 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATATCAATTGGCGGACT+TGG | 0.336187 | 8.4:+7265464 | MS.gene57663:CDS |
| ATAAAATTTGATGTAGAATC+TGG | 0.367314 | 8.4:-7265558 | None:intergenic |
| CAATGTATAGTTTAGTTTGA+AGG | 0.396267 | 8.4:-7265529 | None:intergenic |
| GGTGGAGTGCCTATTGCATT+TGG | 0.425683 | 8.4:+7265410 | MS.gene57663:CDS |
| ACTGAAGAGCCGGCTGCCTA+TGG | 0.457767 | 8.4:+7265437 | MS.gene57663:CDS |
| TCAGTCCCACCAAATGCAAT+AGG | 0.463249 | 8.4:-7265419 | None:intergenic |
| TATGGAGAGCTGATATCAAT+TGG | 0.477027 | 8.4:+7265455 | MS.gene57663:CDS |
| GGAGTGCCTATTGCATTTGG+TGG | 0.518848 | 8.4:+7265413 | MS.gene57663:CDS |
| AATTGATATCAGCTCTCCAT+AGG | 0.553233 | 8.4:-7265453 | None:intergenic |
| GAGTGCCTATTGCATTTGGT+GGG | 0.560113 | 8.4:+7265414 | MS.gene57663:CDS |
| GGAGAGCTGATATCAATTGG+CGG | 0.574098 | 8.4:+7265458 | MS.gene57663:CDS |
| ATCAGCTCTCCATAGGCAGC+CGG | 0.584190 | 8.4:-7265446 | None:intergenic |
| ATTTGGTGGGACTGAAGAGC+CGG | 0.598688 | 8.4:+7265427 | MS.gene57663:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATTTGATGTAGAATC+TGG | - | chr8.4:7265561-7265580 | None:intergenic | 20.0% |
| !! | TATGTGATGATTTTGTTGAA+TGG | + | chr8.4:7265389-7265408 | MS.gene57663:CDS | 25.0% |
| !!! | CAATGTATAGTTTAGTTTGA+AGG | - | chr8.4:7265532-7265551 | None:intergenic | 25.0% |
| AATTGATATCAGCTCTCCAT+AGG | - | chr8.4:7265456-7265475 | None:intergenic | 35.0% | |
| TATGGAGAGCTGATATCAAT+TGG | + | chr8.4:7265455-7265474 | MS.gene57663:CDS | 35.0% | |
| ! | CTCAGTTTTGCATTAACACT+TGG | - | chr8.4:7265491-7265510 | None:intergenic | 35.0% |
| ! | GTGATGATTTTGTTGAATGG+TGG | + | chr8.4:7265392-7265411 | MS.gene57663:CDS | 35.0% |
| GAGTGCCTATTGCATTTGGT+GGG | + | chr8.4:7265414-7265433 | MS.gene57663:CDS | 45.0% | |
| GGAGAGCTGATATCAATTGG+CGG | + | chr8.4:7265458-7265477 | MS.gene57663:CDS | 45.0% | |
| TCAGTCCCACCAAATGCAAT+AGG | - | chr8.4:7265422-7265441 | None:intergenic | 45.0% | |
| !! | CTGATATCAATTGGCGGACT+TGG | + | chr8.4:7265464-7265483 | MS.gene57663:CDS | 45.0% |
| ! | ATTTGGTGGGACTGAAGAGC+CGG | + | chr8.4:7265427-7265446 | MS.gene57663:CDS | 50.0% |
| ! | GGAGTGCCTATTGCATTTGG+TGG | + | chr8.4:7265413-7265432 | MS.gene57663:CDS | 50.0% |
| ! | GGTGGAGTGCCTATTGCATT+TGG | + | chr8.4:7265410-7265429 | MS.gene57663:CDS | 50.0% |
| ATCAGCTCTCCATAGGCAGC+CGG | - | chr8.4:7265449-7265468 | None:intergenic | 55.0% | |
| ACTGAAGAGCCGGCTGCCTA+TGG | + | chr8.4:7265437-7265456 | MS.gene57663:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 7265389 | 7265589 | 7265389 | ID=MS.gene57663 |
| chr8.4 | mRNA | 7265389 | 7265589 | 7265389 | ID=MS.gene57663.t1;Parent=MS.gene57663 |
| chr8.4 | exon | 7265389 | 7265589 | 7265389 | ID=MS.gene57663.t1.exon1;Parent=MS.gene57663.t1 |
| chr8.4 | CDS | 7265389 | 7265589 | 7265389 | ID=cds.MS.gene57663.t1;Parent=MS.gene57663.t1 |
| Gene Sequence |
| Protein sequence |
