AlfalfaGEDB | MS.gene57756

M. sativa cultivar XinJiangDaYe / MS.gene57756

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57756.t1	XP_013448012.1	98	202	4	0	1	202	204	405	6.90E-114	419.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57756.t1	Q9LI89	48.0	200	102	2	2	199	222	421	7.4e-48	191.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57756.t1	A0A072TX07	98.0	202	4	0	1	202	204	405	5.0e-114	419.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene57756	-0.802703	4.95E-49	-1.69E-46
MS.gene049281	MS.gene57756	0.854073	1.57E-61	-1.69E-46
MS.gene049320	MS.gene57756	-0.801452	8.95E-49	-1.69E-46
MS.gene050170	MS.gene57756	-0.810404	1.17E-50	-1.69E-46
MS.gene050381	MS.gene57756	0.826501	2.62E-54	-1.69E-46
MS.gene050496	MS.gene57756	-0.826124	3.22E-54	-1.69E-46
MS.gene050504	MS.gene57756	-0.820907	5.35E-53	-1.69E-46
MS.gene050577	MS.gene57756	-0.808676	2.76E-50	-1.69E-46
MS.gene050612	MS.gene57756	-0.806117	9.61E-50	-1.69E-46
MS.gene050699	MS.gene57756	-0.808781	2.62E-50	-1.69E-46
MS.gene050883	MS.gene57756	0.854833	9.46E-62	-1.69E-46
MS.gene051044	MS.gene57756	0.811531	6.68E-51	-1.69E-46
MS.gene051127	MS.gene57756	0.824121	9.58E-54	-1.69E-46
MS.gene051138	MS.gene57756	0.801186	1.01E-48	-1.69E-46
MS.gene051364	MS.gene57756	0.838338	3.05E-57	-1.69E-46
MS.gene051366	MS.gene57756	0.822615	2.16E-53	-1.69E-46
MS.gene051367	MS.gene57756	0.802289	6.02E-49	-1.69E-46
MS.gene051422	MS.gene57756	0.806069	9.84E-50	-1.69E-46
MS.gene052486	MS.gene57756	-0.803404	3.54E-49	-1.69E-46
MS.gene053203	MS.gene57756	-0.801411	9.12E-49	-1.69E-46
MS.gene053420	MS.gene57756	0.840746	7.22E-58	-1.69E-46
MS.gene053549	MS.gene57756	0.815526	8.84E-52	-1.69E-46
MS.gene053826	MS.gene57756	0.856736	2.63E-62	-1.69E-46
MS.gene054708	MS.gene57756	0.808616	2.84E-50	-1.69E-46
MS.gene05470	MS.gene57756	-0.825513	4.50E-54	-1.69E-46
MS.gene054954	MS.gene57756	-0.803031	4.23E-49	-1.69E-46
MS.gene054959	MS.gene57756	0.840655	7.63E-58	-1.69E-46
MS.gene055139	MS.gene57756	0.803314	3.70E-49	-1.69E-46
MS.gene055154	MS.gene57756	0.832601	8.61E-56	-1.69E-46
MS.gene055362	MS.gene57756	-0.809306	2.02E-50	-1.69E-46
MS.gene055502	MS.gene57756	-0.819068	1.41E-52	-1.69E-46
MS.gene05589	MS.gene57756	-0.81251	4.09E-51	-1.69E-46
MS.gene056218	MS.gene57756	0.805067	1.60E-49	-1.69E-46
MS.gene056310	MS.gene57756	0.806527	7.88E-50	-1.69E-46
MS.gene056311	MS.gene57756	0.820475	6.73E-53	-1.69E-46
MS.gene056504	MS.gene57756	-0.830429	2.95E-55	-1.69E-46
MS.gene056692	MS.gene57756	-0.802383	5.76E-49	-1.69E-46
MS.gene056748	MS.gene57756	0.809555	1.79E-50	-1.69E-46
MS.gene057014	MS.gene57756	0.803152	4.00E-49	-1.69E-46
MS.gene057261	MS.gene57756	0.801959	7.04E-49	-1.69E-46
MS.gene057499	MS.gene57756	0.819064	1.41E-52	-1.69E-46
MS.gene058068	MS.gene57756	0.814331	1.63E-51	-1.69E-46
MS.gene058099	MS.gene57756	0.819759	9.81E-53	-1.69E-46
MS.gene058109	MS.gene57756	0.816038	6.79E-52	-1.69E-46
MS.gene058111	MS.gene57756	0.834638	2.67E-56	-1.69E-46
MS.gene058171	MS.gene57756	0.807327	5.34E-50	-1.69E-46
MS.gene058175	MS.gene57756	0.804101	2.54E-49	-1.69E-46
MS.gene058268	MS.gene57756	0.830563	2.74E-55	-1.69E-46
MS.gene058269	MS.gene57756	0.810473	1.13E-50	-1.69E-46
MS.gene058419	MS.gene57756	-0.804264	2.35E-49	-1.69E-46
MS.gene058493	MS.gene57756	0.806549	7.79E-50	-1.69E-46
MS.gene058603	MS.gene57756	0.811753	5.98E-51	-1.69E-46
MS.gene059022	MS.gene57756	-0.817419	3.33E-52	-1.69E-46
MS.gene059023	MS.gene57756	-0.801194	1.01E-48	-1.69E-46
MS.gene059223	MS.gene57756	0.800437	1.44E-48	-1.69E-46
MS.gene059677	MS.gene57756	-0.807032	6.16E-50	-1.69E-46
MS.gene059865	MS.gene57756	-0.808958	2.40E-50	-1.69E-46
MS.gene059953	MS.gene57756	0.841648	4.18E-58	-1.69E-46
MS.gene059955	MS.gene57756	0.80909	2.25E-50	-1.69E-46
MS.gene060358	MS.gene57756	0.802952	4.39E-49	-1.69E-46
MS.gene060595	MS.gene57756	0.831499	1.61E-55	-1.69E-46
MS.gene060762	MS.gene57756	-0.801685	8.01E-49	-1.69E-46
MS.gene060778	MS.gene57756	-0.808108	3.64E-50	-1.69E-46
MS.gene06077	MS.gene57756	-0.845905	3.03E-59	-1.69E-46
MS.gene060982	MS.gene57756	-0.80219	6.31E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACCTATAGCGAAATTT+TGG	0.201938	7.4:+72162844	MS.gene57756:CDS
GACAAGAGATTCTATGTTAT+TGG	0.250445	7.4:+72162959	MS.gene57756:CDS
TTTCTCTTTGAATTCATATT+CGG	0.325901	7.4:-72162914	None:intergenic
AGCTGATGCACAAGGCGGTT+GGG	0.354563	7.4:+72163219	MS.gene57756:CDS
GTTGAAAGACATTCCACTTT+CGG	0.373291	7.4:+72163066	MS.gene57756:CDS
TACAGCTTAATCATTTCATT+AGG	0.374102	7.4:+72163389	MS.gene57756:CDS
GGTGATGAGTTACTTGTTAT+CGG	0.384004	7.4:+72163262	MS.gene57756:CDS
CATTTCAATTTCTCACCCTC+AGG	0.390524	7.4:-72163343	None:intergenic
ATTTGAAAGGGATCAATAAC+TGG	0.407898	7.4:+72163170	MS.gene57756:CDS
AATTTCTCACCCTCAGGATC+AGG	0.424534	7.4:-72163337	None:intergenic
TTCGCATAAAGAACGAGCTT+TGG	0.431074	7.4:+72163297	MS.gene57756:CDS
GGAATGTCTTTCAACATGTC+AGG	0.452600	7.4:-72163058	None:intergenic
TTTGAATTCATATTCGGTAA+AGG	0.467260	7.4:-72162908	None:intergenic
GCTGATGCACAAGGCGGTTG+GGG	0.472323	7.4:+72163220	MS.gene57756:CDS
CTGAGGGTGAGAAATTGAAA+TGG	0.473233	7.4:+72163344	MS.gene57756:CDS
ATTCTATGTTATTGGTGGAC+AGG	0.479496	7.4:+72162967	MS.gene57756:CDS
AGCTGAAAGTGTATTTGAAA+GGG	0.490575	7.4:+72163158	MS.gene57756:CDS
GAGCTGAAAGTGTATTTGAA+AGG	0.497856	7.4:+72163157	MS.gene57756:CDS
TAGGAATTGTGCTGTGATGA+TGG	0.506085	7.4:+72163408	MS.gene57756:CDS
GAGCTGATGCACAAGGCGGT+TGG	0.506566	7.4:+72163218	MS.gene57756:CDS
CCTTGTGCATCAGCTCTCAC+AGG	0.510167	7.4:-72163211	None:intergenic
AAATGGAAGCAAATTGATTG+TGG	0.518481	7.4:+72163361	MS.gene57756:CDS
GGTGTCCAAAATTTCGCTAT+AGG	0.532541	7.4:-72162849	None:intergenic
TACTTGTTGTCCTGATCCTG+AGG	0.533715	7.4:+72163327	MS.gene57756:CDS
AAGAGATTCTATGTTATTGG+TGG	0.534243	7.4:+72162962	MS.gene57756:CDS
TCGACGAAGAAACAAACACT+TGG	0.563005	7.4:+72163026	MS.gene57756:CDS
CGTTCTTTATGCGAAACAGA+AGG	0.588464	7.4:-72163289	None:intergenic
TATTAACAACAGCTATCAGA+GGG	0.594157	7.4:-72163102	None:intergenic
GGAGAGTGCGAATCCGAAAG+TGG	0.596088	7.4:-72163079	None:intergenic
ACTTGTTGTCCTGATCCTGA+GGG	0.606724	7.4:+72163328	MS.gene57756:CDS
GGTGTTGCATTTAAATCACT+TGG	0.608539	7.4:+72163241	MS.gene57756:CDS
AGGAATTGTGCTGTGATGAT+GGG	0.616810	7.4:+72163409	MS.gene57756:CDS
TTATTAACAACAGCTATCAG+AGG	0.633695	7.4:-72163103	None:intergenic
CCTGTGAGAGCTGATGCACA+AGG	0.635244	7.4:+72163211	MS.gene57756:CDS
ATTAACAACAGCTATCAGAG+GGG	0.676676	7.4:-72163101	None:intergenic
TGGACAGGACGAGAATCGAA+AGG	0.677851	7.4:+72162982	MS.gene57756:CDS
TTTCGAATCATATTTCTCAG+CGG	0.689567	7.4:-72162870	None:intergenic
TTAACAACAGCTATCAGAGG+GGG	0.692839	7.4:-72163100	None:intergenic
GTGAGAGCTGATGCACAAGG+CGG	0.771409	7.4:+72163214	MS.gene57756:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCAATAACTGGAAAAAATT+AGG	+	chr7.4:72163181-72163200	MS.gene57756:CDS	20.0%
!!	TTTCTCTTTGAATTCATATT+CGG	-	chr7.4:72162917-72162936	None:intergenic	20.0%
!	ATGATTCGAAAACAAAAACT+TGG	+	chr7.4:72162882-72162901	MS.gene57756:CDS	25.0%
!	TACAGCTTAATCATTTCATT+AGG	+	chr7.4:72163389-72163408	MS.gene57756:CDS	25.0%
!	TTTGAATTCATATTCGGTAA+AGG	-	chr7.4:72162911-72162930	None:intergenic	25.0%
!!	TCAAAGAGAAAATTTTGTTC+AGG	+	chr7.4:72162926-72162945	MS.gene57756:CDS	25.0%
	AAATGGAAGCAAATTGATTG+TGG	+	chr7.4:72163361-72163380	MS.gene57756:CDS	30.0%
	AAGAGATTCTATGTTATTGG+TGG	+	chr7.4:72162962-72162981	MS.gene57756:CDS	30.0%
	ATTTGAAAGGGATCAATAAC+TGG	+	chr7.4:72163170-72163189	MS.gene57756:CDS	30.0%
	GACAAGAGATTCTATGTTAT+TGG	+	chr7.4:72162959-72162978	MS.gene57756:CDS	30.0%
	TATTAACAACAGCTATCAGA+GGG	-	chr7.4:72163105-72163124	None:intergenic	30.0%
	TTATTAACAACAGCTATCAG+AGG	-	chr7.4:72163106-72163125	None:intergenic	30.0%
	TTTCGAATCATATTTCTCAG+CGG	-	chr7.4:72162873-72162892	None:intergenic	30.0%
!	AAACACCTATAGCGAAATTT+TGG	+	chr7.4:72162844-72162863	MS.gene57756:CDS	30.0%
!	AGCTGAAAGTGTATTTGAAA+GGG	+	chr7.4:72163158-72163177	MS.gene57756:CDS	30.0%
!	ATTTTGTTCAGGATGTTACA+TGG	+	chr7.4:72162937-72162956	MS.gene57756:CDS	30.0%
	ATTAACAACAGCTATCAGAG+GGG	-	chr7.4:72163104-72163123	None:intergenic	35.0%
	GTTGAAAGACATTCCACTTT+CGG	+	chr7.4:72163066-72163085	MS.gene57756:CDS	35.0%
!	ATTCTATGTTATTGGTGGAC+AGG	+	chr7.4:72162967-72162986	MS.gene57756:CDS	35.0%
!	GAGCTGAAAGTGTATTTGAA+AGG	+	chr7.4:72163157-72163176	MS.gene57756:CDS	35.0%
!	GGTGTTGCATTTAAATCACT+TGG	+	chr7.4:72163241-72163260	MS.gene57756:CDS	35.0%
!!	GGTGATGAGTTACTTGTTAT+CGG	+	chr7.4:72163262-72163281	MS.gene57756:CDS	35.0%
	AGGAATTGTGCTGTGATGAT+GGG	+	chr7.4:72163409-72163428	MS.gene57756:CDS	40.0%
	CATTTCAATTTCTCACCCTC+AGG	-	chr7.4:72163346-72163365	None:intergenic	40.0%
	CGTTCTTTATGCGAAACAGA+AGG	-	chr7.4:72163292-72163311	None:intergenic	40.0%
	CTGAGGGTGAGAAATTGAAA+TGG	+	chr7.4:72163344-72163363	MS.gene57756:CDS	40.0%
	GGAATGTCTTTCAACATGTC+AGG	-	chr7.4:72163061-72163080	None:intergenic	40.0%
	GGTGTCCAAAATTTCGCTAT+AGG	-	chr7.4:72162852-72162871	None:intergenic	40.0%
	TAGGAATTGTGCTGTGATGA+TGG	+	chr7.4:72163408-72163427	MS.gene57756:CDS	40.0%
	TCGACGAAGAAACAAACACT+TGG	+	chr7.4:72163026-72163045	MS.gene57756:CDS	40.0%
	TTAACAACAGCTATCAGAGG+GGG	-	chr7.4:72163103-72163122	None:intergenic	40.0%
	TTCGCATAAAGAACGAGCTT+TGG	+	chr7.4:72163297-72163316	MS.gene57756:CDS	40.0%
	AATTTCTCACCCTCAGGATC+AGG	-	chr7.4:72163340-72163359	None:intergenic	45.0%
!!	ACTTGTTGTCCTGATCCTGA+GGG	+	chr7.4:72163328-72163347	MS.gene57756:CDS	45.0%
!!	TACTTGTTGTCCTGATCCTG+AGG	+	chr7.4:72163327-72163346	MS.gene57756:CDS	45.0%
	TGGACAGGACGAGAATCGAA+AGG	+	chr7.4:72162982-72163001	MS.gene57756:CDS	50.0%
	AGCTGATGCACAAGGCGGTT+GGG	+	chr7.4:72163219-72163238	MS.gene57756:CDS	55.0%
	CCTGTGAGAGCTGATGCACA+AGG	+	chr7.4:72163211-72163230	MS.gene57756:CDS	55.0%
	CCTTGTGCATCAGCTCTCAC+AGG	-	chr7.4:72163214-72163233	None:intergenic	55.0%
	GTGAGAGCTGATGCACAAGG+CGG	+	chr7.4:72163214-72163233	MS.gene57756:CDS	55.0%
!	GGAGAGTGCGAATCCGAAAG+TGG	-	chr7.4:72163082-72163101	None:intergenic	55.0%
	GAGCTGATGCACAAGGCGGT+TGG	+	chr7.4:72163218-72163237	MS.gene57756:CDS	60.0%
	GCTGATGCACAAGGCGGTTG+GGG	+	chr7.4:72163220-72163239	MS.gene57756:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	72162831	72163441	72162831	ID=MS.gene57756
chr7.4	mRNA	72162831	72163441	72162831	ID=MS.gene57756.t1;Parent=MS.gene57756
chr7.4	exon	72162831	72163441	72162831	ID=MS.gene57756.t1.exon1;Parent=MS.gene57756.t1
chr7.4	CDS	72162831	72163441	72162831	ID=cds.MS.gene57756.t1;Parent=MS.gene57756.t1

Gene Sequence

>MS.gene57756

GTGGTTTGGAAACAAACACCTATAGCGAAATTTTGGACACCGCTGAGAAATATGATTCGAAAACAAAAACTTGGAAACCTTTACCGAATATGAATTCAAAGAGAAAATTTTGTTCAGGATGTTACATGGACAAGAGATTCTATGTTATTGGTGGACAGGACGAGAATCGAAAGGATCTCATTTCGTGTGAGTTTTTCGACGAAGAAACAAACACTTGGAATTTGATTCCTGACATGTTGAAAGACATTCCACTTTCGGATTCGCACTCTCCCCCTCTGATAGCTGTTGTTAATAATGAACTTTATACACTCGATGCTTCTACTAATGAGCTGAAAGTGTATTTGAAAGGGATCAATAACTGGAAAAAATTAGGTGTTGCGCCTGTGAGAGCTGATGCACAAGGCGGTTGGGGTGTTGCATTTAAATCACTTGGTGATGAGTTACTTGTTATCGGTGCACCTTCTGTTTCGCATAAAGAACGAGCTTTGGCGATTTATACTTGTTGTCCTGATCCTGAGGGTGAGAAATTGAAATGGAAGCAAATTGATTGTGGAAGTGTACAGCTTAATCATTTCATTAGGAATTGTGCTGTGATGATGGGAAGTACTTGA

Protein sequence

>MS.gene57756.t1

VVWKQTPIAKFWTPLRNMIRKQKLGNLYRIUIQRENFVQDVTWTRDSMLLVDRTRIERISFRVSFSTKKQTLGIUFLTCUKTFHFRIRTLPLUULLLIMNFIHSMLLLMSUKCIUKGSITGKNUVLRLUELMHKAVGVLHLNHLVMSYLLSVHLLFRIKNELWRFILVVLILRVRNUNGSKLIVEVYSLIISLGIVLUUWEVL