Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58983.t1 | PNX75878.1 | 61.1 | 90 | 31 | 1 | 1 | 86 | 1 | 90 | 1.20E-14 | 89 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58983.t1 | A0A2K3LBG3 | 61.1 | 90 | 31 | 1 | 1 | 86 | 1 | 90 | 8.6e-15 | 89.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene58983 | 0.847981 | 8.20E-60 | -1.69E-46 |
| MS.gene049396 | MS.gene58983 | 0.80047 | 1.42E-48 | -1.69E-46 |
| MS.gene049552 | MS.gene58983 | 0.806239 | 9.06E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene58983 | 0.801436 | 9.01E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene58983 | 0.80309 | 4.11E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene58983 | 0.847026 | 1.50E-59 | -1.69E-46 |
| MS.gene050216 | MS.gene58983 | 0.813867 | 2.06E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene58983 | 0.840478 | 8.49E-58 | -1.69E-46 |
| MS.gene050381 | MS.gene58983 | -0.80956 | 1.78E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene58983 | 0.860987 | 1.41E-63 | -1.69E-46 |
| MS.gene050502 | MS.gene58983 | 0.807244 | 5.56E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene58983 | 0.825906 | 3.63E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene58983 | 0.811117 | 8.22E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene58983 | 0.803272 | 3.77E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene58983 | 0.82953 | 4.89E-55 | -1.69E-46 |
| MS.gene050697 | MS.gene58983 | 0.826562 | 2.53E-54 | -1.69E-46 |
| MS.gene050868 | MS.gene58983 | 0.854002 | 1.64E-61 | -1.69E-46 |
| MS.gene050871 | MS.gene58983 | 0.857213 | 1.90E-62 | -1.69E-46 |
| MS.gene050986 | MS.gene58983 | 0.817133 | 3.86E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene58983 | -0.800708 | 1.27E-48 | -1.69E-46 |
| MS.gene051268 | MS.gene58983 | 0.817471 | 3.24E-52 | -1.69E-46 |
| MS.gene051509 | MS.gene58983 | 0.821107 | 4.81E-53 | -1.69E-46 |
| MS.gene051560 | MS.gene58983 | 0.813204 | 2.88E-51 | -1.69E-46 |
| MS.gene051874 | MS.gene58983 | 0.806509 | 7.95E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene58983 | 0.822902 | 1.85E-53 | -1.69E-46 |
| MS.gene052164 | MS.gene58983 | 0.818348 | 2.05E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene58983 | 0.824196 | 9.20E-54 | -1.69E-46 |
| MS.gene052486 | MS.gene58983 | 0.826291 | 2.94E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene58983 | 0.803093 | 4.11E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene58983 | 0.803157 | 3.99E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene58983 | 0.807789 | 4.26E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene58983 | 0.816168 | 6.35E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene58983 | -0.826021 | 3.41E-54 | -1.69E-46 |
| MS.gene053480 | MS.gene58983 | 0.801142 | 1.04E-48 | -1.69E-46 |
| MS.gene053574 | MS.gene58983 | 0.8123 | 4.54E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene58983 | 0.817043 | 4.05E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene58983 | 0.808403 | 3.15E-50 | -1.69E-46 |
| MS.gene053826 | MS.gene58983 | -0.800271 | 1.56E-48 | -1.69E-46 |
| MS.gene054003 | MS.gene58983 | 0.810339 | 1.21E-50 | -1.69E-46 |
| MS.gene054595 | MS.gene58983 | 0.833546 | 5.01E-56 | -1.69E-46 |
| MS.gene054954 | MS.gene58983 | 0.820509 | 6.61E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene58983 | 0.819271 | 1.27E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene58983 | 0.833126 | 6.38E-56 | -1.69E-46 |
| MS.gene056166 | MS.gene58983 | 0.813487 | 2.50E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene58983 | -0.801737 | 7.82E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene58983 | 0.827765 | 1.31E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene58983 | 0.855172 | 7.54E-62 | -1.69E-46 |
| MS.gene056510 | MS.gene58983 | 0.815991 | 6.96E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene58983 | 0.809459 | 1.87E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene58983 | 0.821796 | 3.34E-53 | -1.69E-46 |
| MS.gene05787 | MS.gene58983 | 0.828324 | 9.58E-55 | -1.69E-46 |
| MS.gene058157 | MS.gene58983 | 0.838893 | 2.19E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene58983 | -0.830015 | 3.73E-55 | -1.69E-46 |
| MS.gene058173 | MS.gene58983 | -0.830217 | 3.33E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene58983 | -0.82913 | 6.12E-55 | -1.69E-46 |
| MS.gene058269 | MS.gene58983 | -0.80711 | 5.93E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene58983 | 0.822996 | 1.76E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene58983 | 0.81197 | 5.36E-51 | -1.69E-46 |
| MS.gene059045 | MS.gene58983 | -0.812863 | 3.42E-51 | -1.69E-46 |
| MS.gene059223 | MS.gene58983 | -0.801892 | 7.27E-49 | -1.69E-46 |
| MS.gene059367 | MS.gene58983 | 0.835793 | 1.36E-56 | -1.69E-46 |
| MS.gene059680 | MS.gene58983 | 0.802311 | 5.96E-49 | -1.69E-46 |
| MS.gene059837 | MS.gene58983 | 0.810236 | 1.27E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene58983 | 0.845531 | 3.83E-59 | -1.69E-46 |
| MS.gene06000 | MS.gene58983 | 0.864359 | 1.29E-64 | -1.69E-46 |
| MS.gene060187 | MS.gene58983 | -0.809687 | 1.67E-50 | -1.69E-46 |
| MS.gene060593 | MS.gene58983 | -0.820806 | 5.65E-53 | -1.69E-46 |
| MS.gene060595 | MS.gene58983 | -0.847914 | 8.55E-60 | -1.69E-46 |
| MS.gene060778 | MS.gene58983 | 0.897621 | 1.18E-76 | -1.69E-46 |
| MS.gene06077 | MS.gene58983 | 0.825915 | 3.61E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene58983 | 0.829117 | 6.16E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58983.t1 | MTR_3g113890 | 88.372 | 43 | 5 | 0 | 1 | 43 | 1 | 43 | 5.60e-20 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 5.2:+40034988 | None:intergenic |
| TCTCCATCTTGTTCATGCTT+TGG | 0.293177 | 5.2:-40035685 | MS.gene58983:CDS |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 5.2:+40034974 | None:intergenic |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 5.2:+40034952 | None:intergenic |
| TGCGTGCCACCAGGAACTTC+TGG | 0.331803 | 5.2:-40035014 | MS.gene58983:CDS |
| TCTTGGTTACCAGAAGTTCC+TGG | 0.400394 | 5.2:+40035005 | None:intergenic |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 5.2:+40034961 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 5.2:+40034965 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 5.2:-40035059 | MS.gene58983:intron |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 5.2:+40034953 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 5.2:+40034956 | None:intergenic |
| TGATGATTCAACTCAATTCA+AGG | 0.508747 | 5.2:+40034930 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+AGG | 0.530335 | 5.2:-40035023 | MS.gene58983:CDS |
| AGCATGAACAAGATGGAGAA+TGG | 0.540714 | 5.2:+40035689 | None:intergenic |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 5.2:+40034975 | None:intergenic |
| TGTTCATGCTTTGGACCAGC+CGG | 0.555470 | 5.2:-40035676 | MS.gene58983:intron |
| GATGATTCAACTCAATTCAA+GGG | 0.567646 | 5.2:+40034931 | None:intergenic |
| GGTCCAAAGCATGAACAAGA+TGG | 0.576917 | 5.2:+40035682 | None:intergenic |
| TGAGTTTCTGCGTGTACATG+CGG | 0.582718 | 5.2:+40035540 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 5.2:-40035043 | MS.gene58983:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 5.2:-40034969 | MS.gene58983:CDS |
| TGGTTACCAGAAGTTCCTGG+TGG | 0.663688 | 5.2:+40035008 | None:intergenic |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 5.2:-40034966 | MS.gene58983:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATGAACTAAACTA+AGG | - | chr5.2:40035279-40035298 | MS.gene58983:intron | 15.0% |
| !! | TGGAAAAAATATAAAAAAAC+TGG | - | chr5.2:40035233-40035252 | MS.gene58983:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | chr5.2:40035213-40035232 | MS.gene58983:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | chr5.2:40035553-40035572 | MS.gene58983:intron | 20.0% |
| !! | GGATAAAAGATTAGTAAAAA+AGG | - | chr5.2:40035393-40035412 | MS.gene58983:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | chr5.2:40035071-40035090 | None:intergenic | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | chr5.2:40035372-40035391 | MS.gene58983:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | chr5.2:40035371-40035390 | MS.gene58983:intron | 25.0% |
| !! | TTAATTACATGTGTTTTCGT+AGG | - | chr5.2:40035107-40035126 | MS.gene58983:intron | 25.0% |
| ! | CTACTTCACACACATATTTT+TGG | + | chr5.2:40035521-40035540 | None:intergenic | 30.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | chr5.2:40035018-40035037 | None:intergenic | 30.0% |
| AGCATGAACAAGATGGAGAA+TGG | + | chr5.2:40034986-40035005 | None:intergenic | 40.0% | |
| TAAAGAAAACGCTTGGAGCT+TGG | + | chr5.2:40035064-40035083 | None:intergenic | 40.0% | |
| TCTCCATCTTGTTCATGCTT+TGG | - | chr5.2:40034987-40035006 | MS.gene58983:CDS | 40.0% | |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | chr5.2:40035156-40035175 | MS.gene58983:intron | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | chr5.2:40035014-40035033 | None:intergenic | 40.0% |
| AACCAGACAGAAGCAGTTGA+TGG | + | chr5.2:40035195-40035214 | None:intergenic | 45.0% | |
| CATAGCAGGGACACATTTCT+TGG | + | chr5.2:40035687-40035706 | None:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | chr5.2:40034993-40035012 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | chr5.2:40035670-40035689 | None:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | chr5.2:40035135-40035154 | None:intergenic | 45.0% | |
| TTCCATCAACTGCTTCTGTC+TGG | - | chr5.2:40035190-40035209 | MS.gene58983:intron | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr5.2:40035587-40035606 | MS.gene58983:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | chr5.2:40035095-40035114 | None:intergenic | 5.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr5.2:40035703-40035722 | MS.gene58983:CDS | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | chr5.2:40035667-40035686 | None:intergenic | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | chr5.2:40034996-40035015 | MS.gene58983:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr5.2:40035629-40035648 | MS.gene58983:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr5.2:40035700-40035719 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr5.2:40035624-40035643 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr5.2:40035569-40035588 | MS.gene58983:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr5.2:40035701-40035720 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr5.2:40035722-40035741 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr5.2:40035706-40035725 | MS.gene58983:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr5.2:40035597-40035616 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr5.2:40035714-40035733 | None:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | chr5.2:40035658-40035677 | MS.gene58983:intron | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr5.2:40035618-40035637 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr5.2:40035710-40035729 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr5.2:40035719-40035738 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr5.2:40035723-40035742 | None:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr5.2:40035613-40035632 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr5.2:40035613-40035632 | MS.gene58983:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | chr5.2:40035649-40035668 | MS.gene58983:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr5.2:40035606-40035625 | MS.gene58983:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr5.2:40035605-40035624 | MS.gene58983:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 40034937 | 40035757 | 40034937 | ID=MS.gene58983 |
| chr5.2 | mRNA | 40034937 | 40035757 | 40034937 | ID=MS.gene58983.t1;Parent=MS.gene58983 |
| chr5.2 | exon | 40035677 | 40035757 | 40035677 | ID=MS.gene58983.t1.exon1;Parent=MS.gene58983.t1 |
| chr5.2 | CDS | 40035677 | 40035757 | 40035677 | ID=cds.MS.gene58983.t1;Parent=MS.gene58983.t1 |
| chr5.2 | exon | 40035517 | 40035565 | 40035517 | ID=MS.gene58983.t1.exon2;Parent=MS.gene58983.t1 |
| chr5.2 | CDS | 40035517 | 40035565 | 40035517 | ID=cds.MS.gene58983.t1;Parent=MS.gene58983.t1 |
| chr5.2 | exon | 40034937 | 40035067 | 40034937 | ID=MS.gene58983.t1.exon3;Parent=MS.gene58983.t1 |
| chr5.2 | CDS | 40034937 | 40035067 | 40034937 | ID=cds.MS.gene58983.t1;Parent=MS.gene58983.t1 |
| Gene Sequence |
| Protein sequence |
