AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60208

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60208.t1 MTR_4g097090 92.544 456 34 0 1 456 1 456 0.0 873
MS.gene60208.t1 MTR_4g127650 78.444 450 94 3 9 457 10 457 0.0 725
MS.gene60208.t1 MTR_3g026950 42.506 447 237 9 17 452 10 447 2.01e-105 321
MS.gene60208.t1 MTR_3g049150 40.087 459 255 7 9 458 2 449 1.57e-104 319
MS.gene60208.t1 MTR_4g007540 37.360 447 254 9 9 450 3 428 5.07e-94 291
MS.gene60208.t1 MTR_2g064495 37.194 449 255 9 9 452 3 429 4.27e-91 283
MS.gene60208.t1 MTR_4g013050 38.913 460 224 14 3 456 2 410 8.71e-91 282
MS.gene60208.t1 MTR_8g075610 38.480 421 217 10 41 450 26 415 1.91e-90 281
MS.gene60208.t1 MTR_2g105330 36.971 449 256 9 9 452 3 429 4.30e-89 278
MS.gene60208.t1 MTR_7g085930 34.764 466 268 13 9 452 6 457 6.59e-74 240
MS.gene60208.t1 MTR_7g086000 35.398 452 274 10 17 456 12 457 3.68e-73 238
MS.gene60208.t1 MTR_7g085990 33.691 466 273 15 9 452 2 453 1.38e-69 229
MS.gene60208.t1 MTR_3g049140 43.277 238 132 3 215 452 6 240 3.11e-61 200
MS.gene60208.t1 MTR_8g096530 30.022 453 282 11 15 462 13 435 8.62e-50 176
MS.gene60208.t1 MTR_7g027175 30.542 406 241 11 15 411 22 395 1.79e-46 167
MS.gene60208.t1 MTR_1g053315 29.176 449 281 13 19 453 33 458 2.60e-45 164
MS.gene60208.t1 MTR_3g098980 30.348 402 242 13 17 411 22 392 2.49e-44 161
MS.gene60208.t1 MTR_7g084940 25.764 458 301 10 9 456 16 444 5.22e-37 141
MS.gene60208.t1 MTR_7g114520 28.481 474 291 16 1 456 1 444 5.36e-35 136
MS.gene60208.t1 MTR_7g056087 23.451 452 308 12 17 455 12 438 3.37e-32 127
MS.gene60208.t1 MTR_7g114470 26.077 441 275 13 34 458 37 442 3.65e-32 128
MS.gene60208.t1 MTR_1g040175 27.628 409 251 12 17 413 17 392 6.63e-32 127
MS.gene60208.t1 MTR_3g071320 26.477 457 290 15 7 448 3 428 9.15e-32 126
MS.gene60208.t1 MTR_1g081660 26.966 445 264 15 30 452 39 444 1.32e-31 126
MS.gene60208.t1 MTR_6g045553 26.141 482 275 13 6 441 11 457 1.42e-31 126
MS.gene60208.t1 MTR_7g084910 25.845 414 259 9 2 409 10 381 1.49e-31 125
MS.gene60208.t1 MTR_5g076700 25.173 433 280 13 34 452 36 438 3.35e-31 125
MS.gene60208.t1 MTR_5g081960 25.646 503 293 16 1 449 1 476 4.29e-31 125
MS.gene60208.t1 MTR_7g114490 25.408 429 277 12 34 449 36 434 7.01e-31 125
MS.gene60208.t1 MTR_7g114560 25.275 455 294 14 17 456 18 441 2.23e-30 123
MS.gene60208.t1 MTR_1g040215 27.674 430 246 18 6 411 2 390 2.88e-30 122
MS.gene60208.t1 MTR_5g090830 25.974 462 298 14 19 456 23 464 3.48e-30 122
MS.gene60208.t1 MTR_4g015180 26.411 496 283 19 1 441 1 469 5.67e-30 122
MS.gene60208.t1 MTR_5g090810 25.862 464 292 16 19 454 23 462 6.93e-30 121
MS.gene60208.t1 MTR_4g055170 26.556 482 295 18 4 456 7 458 1.74e-29 120
MS.gene60208.t1 MTR_7g085960 33.503 197 111 4 262 456 12 190 2.12e-29 114
MS.gene60208.t1 MTR_7g114550 25.172 437 282 13 34 456 36 441 2.35e-29 120
MS.gene60208.t1 MTR_3g112180 28.148 405 235 14 65 449 61 429 6.75e-29 119
MS.gene60208.t1 MTR_1g040195 26.681 461 271 16 1 448 1 407 1.12e-28 117
MS.gene60208.t1 MTR_3g070170 25.410 488 272 15 3 440 6 451 1.44e-28 118
MS.gene60208.t1 MTR_8g079970 26.872 454 281 18 17 456 11 427 1.54e-28 117
MS.gene60208.t1 MTR_3g101960 27.252 444 267 16 30 449 29 440 2.06e-27 114
MS.gene60208.t1 MTR_2g015430 26.500 400 259 10 63 450 56 432 1.32e-26 112
MS.gene60208.t1 MTR_8g041770 25.110 454 274 17 18 447 18 429 1.35e-26 112
MS.gene60208.t1 MTR_3g102020 27.500 400 238 13 61 440 65 432 1.72e-26 111
MS.gene60208.t1 MTR_5g084870 26.622 447 278 19 17 452 11 418 1.90e-26 111
MS.gene60208.t1 MTR_8g079950 26.247 461 263 17 22 456 18 427 2.80e-26 111
MS.gene60208.t1 MTR_3g101970 25.727 447 275 16 30 457 29 437 5.53e-26 110
MS.gene60208.t1 MTR_3g102010 26.291 426 276 15 30 440 30 432 1.45e-25 108
MS.gene60208.t1 MTR_8g079980 26.126 444 289 16 16 451 10 422 2.74e-25 108
MS.gene60208.t1 MTR_3g086020 27.512 418 219 19 17 411 13 369 2.85e-25 107
MS.gene60208.t1 MTR_1g094790 26.366 421 261 16 30 432 26 415 6.36e-25 107
MS.gene60208.t1 MTR_1g040320 25.277 451 257 14 1 438 1 384 1.01e-24 106
MS.gene60208.t1 MTR_5g084850 24.169 451 283 14 17 452 11 417 2.59e-24 105
MS.gene60208.t1 MTR_3g101930 25.734 443 276 15 5 421 3 418 9.54e-24 103
MS.gene60208.t1 MTR_3g101900 23.529 459 292 13 16 449 14 438 1.51e-23 103
MS.gene60208.t1 MTR_4g094835 25.445 393 247 13 65 440 64 427 4.13e-23 101
MS.gene60208.t1 MTR_3g450710 23.601 411 268 11 61 446 61 450 6.58e-23 101
MS.gene60208.t1 MTR_5g090880 25.059 427 276 15 56 456 58 466 2.08e-22 100
MS.gene60208.t1 MTR_0190s0020 26.031 388 248 14 65 440 63 423 4.25e-22 99.0
MS.gene60208.t1 MTR_3g450690 23.529 425 271 13 61 456 68 467 5.29e-22 98.6
MS.gene60208.t1 MTR_3g026310 24.236 458 268 18 27 447 21 436 6.33e-22 98.2
MS.gene60208.t1 MTR_3g465990 27.350 351 216 15 12 342 13 344 8.04e-22 98.2
MS.gene60208.t1 MTR_6g053810 27.540 374 231 14 1 352 1 356 9.48e-22 97.8
MS.gene60208.t1 MTR_3g465930 27.729 339 223 12 17 342 18 347 1.93e-21 97.1
MS.gene60208.t1 MTR_4g094838 24.694 409 255 13 45 440 45 413 2.07e-21 96.7
MS.gene60208.t1 MTR_2g045550 39.161 143 76 2 9 151 4 135 3.32e-21 90.1
MS.gene60208.t1 MTR_4g094842 26.716 408 227 16 65 440 62 429 3.64e-21 95.9
MS.gene60208.t1 MTR_1g040315 26.036 338 220 11 4 331 2 319 3.83e-21 95.5
MS.gene60208.t1 MTR_2g045540 43.478 115 53 1 215 329 6 108 7.14e-21 88.2
MS.gene60208.t1 MTR_4g094832 23.380 355 235 9 95 440 85 411 2.89e-20 93.2
MS.gene60208.t1 MTR_7g114960 25.688 327 217 8 45 363 45 353 5.41e-20 92.4
MS.gene60208.t1 MTR_3g088420 24.256 437 279 17 27 447 21 421 6.23e-20 92.0
MS.gene60208.t1 MTR_3g465910 28.150 373 219 18 17 363 18 367 2.82e-19 90.9
MS.gene60208.t1 MTR_3g465950 29.924 264 167 9 65 318 68 323 4.20e-19 89.7
MS.gene60208.t1 MTR_5g026320 24.934 377 238 13 42 405 53 397 5.20e-19 89.7
MS.gene60208.t1 MTR_3g465980 25.862 348 225 13 12 342 13 344 1.23e-18 88.6
MS.gene60208.t1 MTR_7g110610 24.318 440 253 15 17 451 13 377 5.82e-18 85.9
MS.gene60208.t1 MTR_7g014160 24.254 503 306 20 1 457 1 474 5.84e-18 86.7
MS.gene60208.t1 MTR_4g022290 26.942 412 233 20 30 414 30 400 6.76e-18 86.3
MS.gene60208.t1 MTR_8g467580 24.764 424 279 16 17 427 11 407 7.46e-18 85.9
MS.gene60208.t1 MTR_3g028210 23.941 472 313 15 14 458 14 466 8.04e-18 85.9
MS.gene60208.t1 MTR_4g022720 23.762 404 254 14 65 448 55 424 1.15e-17 85.5
MS.gene60208.t1 MTR_4g022800 24.304 395 254 13 65 440 55 423 1.19e-17 85.5
MS.gene60208.t1 MTR_7g026860 23.543 446 256 18 30 440 24 419 7.49e-17 82.8
MS.gene60208.t1 MTR_8g467590 24.000 450 305 20 12 451 6 428 9.45e-17 82.8
MS.gene60208.t1 MTR_7g014200 23.566 488 325 18 1 458 1 470 1.02e-16 82.8
MS.gene60208.t1 MTR_4g094828 25.862 406 238 19 65 440 59 431 1.31e-16 82.4
MS.gene60208.t1 MTR_8g079710 24.390 451 252 22 28 440 27 426 1.46e-16 82.0
MS.gene60208.t1 MTR_7g085970 41.250 80 46 1 102 181 34 112 3.48e-16 75.1
MS.gene60208.t1 MTR_4g022670 22.936 436 277 17 24 440 27 422 2.64e-15 78.2
MS.gene60208.t1 MTR_2g089735 22.943 401 256 12 40 408 37 416 8.30e-15 76.6
MS.gene60208.t1 MTR_7g014610 22.917 480 312 16 17 457 16 476 1.19e-14 76.3
MS.gene60208.t1 MTR_3g465880 25.228 329 216 13 64 376 67 381 2.97e-14 75.1
MS.gene60208.t1 MTR_1g011660 24.036 441 269 17 28 442 25 425 4.16e-14 74.7
MS.gene60208.t1 MTR_4g007460 35.398 113 65 2 79 191 1 105 8.30e-14 69.3
MS.gene60208.t1 MTR_2g036780 23.155 393 223 16 68 431 62 404 1.03e-13 73.2
MS.gene60208.t1 MTR_4g022760 26.316 285 177 9 65 330 55 325 4.74e-13 70.9
MS.gene60208.t1 MTR_7g114510 24.768 323 202 11 147 456 8 302 5.42e-13 70.1
MS.gene60208.t1 MTR_7g114500 35.000 120 73 3 34 151 36 152 1.00e-12 66.2
MS.gene60208.t1 MTR_0042s0190 30.380 158 92 5 258 413 22 163 3.19e-12 66.6
MS.gene60208.t1 MTR_3g100150 22.660 406 248 16 65 449 65 425 4.67e-12 68.2
MS.gene60208.t1 MTR_0042s0190 30.380 158 92 5 258 413 87 228 5.57e-12 67.0
MS.gene60208.t1 MTR_2g089650 24.750 400 247 16 41 408 41 418 8.07e-12 67.4
MS.gene60208.t1 MTR_7g014330 23.020 404 267 13 28 406 24 408 3.78e-11 65.5
MS.gene60208.t1 MTR_6g015850 21.616 458 298 16 28 456 32 457 5.60e-11 64.7
MS.gene60208.t1 MTR_1g035890 23.438 384 236 16 68 433 63 406 5.90e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60208.t1 AT2G19070 53.493 458 193 8 9 456 4 451 2.00e-172 492
MS.gene60208.t1 AT5G48930 37.665 454 251 11 9 454 3 432 8.61e-94 290
MS.gene60208.t1 AT5G57840 31.155 459 282 12 9 456 3 438 1.59e-62 209
MS.gene60208.t1 AT5G41040 31.096 447 272 11 15 454 23 440 9.35e-57 194
MS.gene60208.t1 AT5G41040 31.096 447 272 11 15 454 39 456 1.10e-56 194
MS.gene60208.t1 AT3G48720 30.736 462 272 13 1 450 1 426 3.37e-51 179
MS.gene60208.t1 AT5G63560 31.317 463 262 14 1 449 1 421 1.33e-47 169
MS.gene60208.t1 AT1G65450 27.430 463 292 12 9 457 18 450 7.35e-39 146
MS.gene60208.t1 AT5G17540 27.468 466 294 15 1 452 1 436 2.40e-37 142
MS.gene60208.t1 AT5G07080 27.991 443 293 13 17 452 22 445 3.65e-35 136
MS.gene60208.t1 AT1G27620 26.376 436 278 10 15 442 15 415 1.02e-34 135
MS.gene60208.t1 AT2G40230 25.167 449 300 9 9 449 5 425 1.66e-33 131
MS.gene60208.t1 AT3G26040 28.829 444 272 19 17 450 11 420 2.38e-33 131
MS.gene60208.t1 AT1G03390 27.152 453 283 14 17 448 25 451 4.29e-32 127
MS.gene60208.t1 AT3G03480 26.402 428 264 13 1 411 7 400 7.61e-31 124
MS.gene60208.t1 AT1G32910 25.245 408 265 15 40 433 44 425 2.45e-30 123
MS.gene60208.t1 AT4G31910 27.123 424 251 14 61 453 60 456 3.14e-29 119
MS.gene60208.t1 AT5G23940 25.702 463 281 13 15 446 9 439 2.63e-28 117
MS.gene60208.t1 AT2G25150 26.211 454 302 16 12 452 16 449 6.86e-28 115
MS.gene60208.t1 AT1G24430 27.551 392 233 18 30 411 29 379 9.87e-28 115
MS.gene60208.t1 AT3G50270 25.397 441 264 16 9 420 6 410 1.97e-27 114
MS.gene60208.t1 AT3G30280 28.009 457 253 21 22 451 21 428 3.66e-27 113
MS.gene60208.t1 AT2G39980 26.291 426 238 13 65 440 62 461 6.01e-27 113
MS.gene60208.t1 AT5G16410 24.471 425 262 16 28 432 59 444 1.65e-26 112
MS.gene60208.t1 AT5G01210 25.635 394 248 12 76 440 73 450 2.61e-26 111
MS.gene60208.t1 AT1G78990 24.515 412 273 13 64 461 66 453 1.27e-25 109
MS.gene60208.t1 AT5G07860 26.439 469 295 15 1 449 1 439 2.15e-25 108
MS.gene60208.t1 AT3G50300 26.277 411 259 14 45 436 35 420 3.01e-25 108
MS.gene60208.t1 AT5G67150 26.000 450 285 17 27 457 26 446 2.02e-24 105
MS.gene60208.t1 AT3G62160 23.765 425 280 11 36 450 26 416 3.04e-24 105
MS.gene60208.t1 AT5G42830 26.531 441 272 14 30 449 30 439 3.32e-24 105
MS.gene60208.t1 AT1G24420 29.723 397 203 18 76 449 76 419 9.63e-24 103
MS.gene60208.t1 AT1G24420 29.723 397 203 18 76 449 76 419 9.63e-24 103
MS.gene60208.t1 AT1G28680 26.456 412 263 14 57 454 57 442 2.23e-23 102
MS.gene60208.t1 AT5G38130 25.439 456 281 17 9 441 24 443 6.84e-23 101
MS.gene60208.t1 AT5G47980 28.132 455 262 19 17 448 13 425 9.02e-23 100
MS.gene60208.t1 AT3G47170 25.926 459 301 17 17 452 20 462 2.43e-22 99.8
MS.gene60208.t1 AT5G47950 27.209 430 257 18 13 432 10 393 2.70e-22 99.4
MS.gene60208.t1 AT4G15390 24.831 443 298 13 17 451 16 431 3.32e-22 99.4
MS.gene60208.t1 AT4G15390 24.831 443 298 13 17 451 16 431 3.32e-22 99.4
MS.gene60208.t1 AT3G50280 25.654 382 235 13 59 420 53 405 5.07e-22 98.6
MS.gene60208.t1 AT2G23510 23.971 413 255 14 65 452 69 447 3.60e-21 96.3
MS.gene60208.t1 AT3G29670 25.277 451 281 18 27 452 26 445 4.49e-21 95.9
MS.gene60208.t1 AT5G07870 26.471 476 298 17 1 449 1 451 1.07e-20 94.7
MS.gene60208.t1 AT5G07850 25.926 405 249 14 65 449 70 443 3.22e-17 84.3
MS.gene60208.t1 AT5G67160 24.771 436 258 15 21 426 20 415 4.52e-17 83.6
MS.gene60208.t1 AT3G50280 25.175 286 185 7 141 420 13 275 4.06e-16 79.3
MS.gene60208.t1 AT3G50290 25.926 297 179 10 125 407 2 271 8.66e-16 77.8
MS.gene60208.t1 AT3G29635 24.605 443 289 19 28 448 27 446 1.00e-15 79.7
MS.gene60208.t1 AT4G15400 25.280 447 285 17 13 448 9 417 2.80e-14 75.1
MS.gene60208.t1 AT1G65450 26.407 231 137 5 236 457 80 286 3.31e-14 73.6
MS.gene60208.t1 AT1G65450 32.857 140 89 4 9 146 18 154 3.07e-13 68.2
MS.gene60208.t1 AT3G29590 26.932 453 269 20 28 452 26 444 3.41e-13 71.6
MS.gene60208.t1 AT5G39050 23.214 392 237 14 99 456 106 467 1.43e-11 66.6

Find 116 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTACCAAAAGGGTATTT+TGG 0.102364 8.1:-25444803 MS.gene60208:CDS
CCAATTTATGGACTTGATTT+TGG 0.108014 8.1:-25444530 MS.gene60208:CDS
TGGTTGTCATTGCCAATTTA+TGG 0.127883 8.1:-25444542 MS.gene60208:CDS
CTTGATAAAATGCTCCTTAA+AGG 0.191004 8.1:+25444376 None:intergenic
GATCCATATGGATGCCTTTA+AGG 0.198031 8.1:-25444390 MS.gene60208:CDS
TTAACATTGTTACGACATTT+TGG 0.220212 8.1:+25445115 None:intergenic
AAGCAGTAACCGGTCATATT+TGG 0.239075 8.1:-25444913 MS.gene60208:CDS
GATCAACTTCCTTTATTGTT+AGG 0.251787 8.1:-25445079 MS.gene60208:CDS
CGCTTGGTGTTTGCGTTGAT+TGG 0.258434 8.1:-25444844 MS.gene60208:CDS
CAACTAACCACTCCTAAATT+AGG 0.269802 8.1:+25444563 None:intergenic
TCTGAATTTCCTAACAATAA+AGG 0.299564 8.1:+25445070 None:intergenic
CCAAAATCAAGTCCATAAAT+TGG 0.313583 8.1:+25444530 None:intergenic
ATCTGATCCCATTCGGATAA+TGG 0.324028 8.1:+25445631 None:intergenic
AAATTAGGATTCCAATAAAA+AGG 0.326383 8.1:+25444578 None:intergenic
CGATGATGATGGTTCTATAT+TGG 0.355288 8.1:-25444432 MS.gene60208:CDS
ATGTGGTGAGCCTTTGAAAA+TGG 0.355480 8.1:-25445170 MS.gene60208:CDS
TCCCTACAACTTGAAGATCT+TGG 0.363159 8.1:+25444615 None:intergenic
GCATTCCCAAAATACCCTTT+TGG 0.365952 8.1:+25444797 None:intergenic
AGTAGCCAAAACATCCAAAA+TGG 0.383532 8.1:+25444775 None:intergenic
AACAATAAAGGAAGTTGATC+AGG 0.389462 8.1:+25445082 None:intergenic
GATATAATGTCCAAACCCTT+AGG 0.390597 8.1:-25444740 MS.gene60208:CDS
GAATAACCCCGACCATTACT+AGG 0.405342 8.1:+25444944 None:intergenic
TGATCTCGAATGCAACTCAT+TGG 0.407126 8.1:-25445443 MS.gene60208:CDS
CCACTTGCCGGTCGTTTACA+CGG 0.416496 8.1:-25445484 MS.gene60208:CDS
TTCCAAGATCTTCAAGTTGT+AGG 0.420035 8.1:-25444617 MS.gene60208:CDS
GAATAAACCTAGTAATGGTC+GGG 0.425138 8.1:-25444951 MS.gene60208:CDS
GTCGTAACAATGTTAATGAA+TGG 0.427383 8.1:-25445108 MS.gene60208:CDS
AGGTGAAAAGTCACCTAGTT+CGG 0.428468 8.1:+25445381 None:intergenic
GTGGAACCTAATTTACCAAA+AGG 0.435496 8.1:-25444812 MS.gene60208:CDS
CTAATTTAGGAGTGGTTAGT+TGG 0.435977 8.1:-25444562 MS.gene60208:CDS
TGAAGCATACCCTAAGGGTT+TGG 0.444962 8.1:+25444730 None:intergenic
GGTATGCTTCAAGTAGGATA+AGG 0.453543 8.1:-25444718 MS.gene60208:CDS
TGTAATCAACTTGTGGGATA+AGG 0.455844 8.1:+25445338 None:intergenic
TCGTAACAATGTTAATGAAT+GGG 0.463530 8.1:-25445107 MS.gene60208:CDS
CTTCCTAGTCCCGATGATGA+TGG 0.463543 8.1:-25444443 MS.gene60208:CDS
GATCTCGAATGCAACTCATT+GGG 0.466117 8.1:-25445442 MS.gene60208:CDS
GCTCCTTAAAGGCATCCATA+TGG 0.467393 8.1:+25444387 None:intergenic
TTTATTCCAACTCTCATTTG+CGG 0.467692 8.1:+25444967 None:intergenic
TCCAAGATCTTCAAGTTGTA+GGG 0.468030 8.1:-25444616 MS.gene60208:CDS
AATGATGTATATGTTAGGTT+GGG 0.477497 8.1:-25444677 MS.gene60208:CDS
GGAACACATGAAGAACTTGA+TGG 0.478823 8.1:-25444479 MS.gene60208:CDS
GTATGCTTCAAGTAGGATAA+GGG 0.479099 8.1:-25444717 MS.gene60208:CDS
AATGACCAACCAACCGCGCT+TGG 0.479297 8.1:-25444860 MS.gene60208:CDS
CCGCACCATGTTGGTTCCAT+TGG 0.480928 8.1:+25445661 None:intergenic
CACTTGCCGGTCGTTTACAC+GGG 0.488481 8.1:-25445483 MS.gene60208:CDS
TCGAGGTATGAAGCAGTAAC+CGG 0.488814 8.1:-25444923 MS.gene60208:CDS
TCGTTTACCATTATCCGAAT+GGG 0.493784 8.1:-25445638 MS.gene60208:CDS
TTGTCTGCAAGAGATCCATA+TGG 0.500703 8.1:-25444402 MS.gene60208:CDS
ATATAATGTCCAAACCCTTA+GGG 0.507169 8.1:-25444739 MS.gene60208:CDS
TCCTTTCCCGTGTAAACGAC+CGG 0.508630 8.1:+25445477 None:intergenic
GAATGATGTATATGTTAGGT+TGG 0.512182 8.1:-25444678 MS.gene60208:CDS
GAACCATCATCATCGGGACT+AGG 0.512386 8.1:+25444440 None:intergenic
GATTCCAATCTGATCCCATT+CGG 0.514466 8.1:+25445624 None:intergenic
CCTACTTGAAGCATACCCTA+AGG 0.516468 8.1:+25444724 None:intergenic
CGACCGGCAAGTGGATAAAA+CGG 0.518498 8.1:+25445493 None:intergenic
GGAGTGTGTAATCAACTTGT+GGG 0.520503 8.1:+25445332 None:intergenic
GTCGTTTACCATTATCCGAA+TGG 0.522947 8.1:-25445639 MS.gene60208:CDS
AAGTGAATGATGTATATGTT+AGG 0.524953 8.1:-25444682 MS.gene60208:CDS
CCAATGGAATATCTTCCATC+GGG 0.526580 8.1:+25445311 None:intergenic
ATTCAGATAATGTTGAAGAA+AGG 0.528429 8.1:-25445054 MS.gene60208:CDS
GATCTTCAAGTTGTAGGGAA+TGG 0.530205 8.1:-25444611 MS.gene60208:CDS
TTGGTGTTTGCGTTGATTGG+AGG 0.530584 8.1:-25444841 MS.gene60208:CDS
TAACTGTAATATTCCGAAGA+AGG 0.531568 8.1:+25445361 None:intergenic
TCGTTAACTTTATCCGAACT+AGG 0.531806 8.1:-25445394 MS.gene60208:CDS
AAAATGAAATGCAACGCGCT+TGG 0.536125 8.1:+25445211 None:intergenic
TGGAACCTAATTTACCAAAA+GGG 0.539334 8.1:-25444811 MS.gene60208:CDS
GTTGTAGGGAATGGTGAGAA+GGG 0.540329 8.1:-25444602 MS.gene60208:CDS
ATCTCACATGCAGTTGCAGA+TGG 0.540526 8.1:-25445235 MS.gene60208:CDS
CCCGATGGAAGATATTCCAT+TGG 0.541022 8.1:-25445311 MS.gene60208:CDS
CTACTTGAAGCATACCCTAA+GGG 0.541120 8.1:+25444725 None:intergenic
AATATAGAACCATCATCATC+GGG 0.541670 8.1:+25444434 None:intergenic
AAGGAGGTTTATATGGGGCT+AGG 0.542309 8.1:-25444500 MS.gene60208:CDS
GGAATAAACCTAGTAATGGT+CGG 0.543778 8.1:-25444952 MS.gene60208:CDS
CCGTGTAAACGACCGGCAAG+TGG 0.543864 8.1:+25445484 None:intergenic
ACCAATGGAATATCTTCCAT+CGG 0.544804 8.1:+25445310 None:intergenic
CAATATAGAACCATCATCAT+CGG 0.546460 8.1:+25444433 None:intergenic
ACGCAAACACCAAGCGCGGT+TGG 0.546925 8.1:+25444851 None:intergenic
GGGTGGTAAAAGTATATTGT+TGG 0.548512 8.1:+25445589 None:intergenic
ATGTTTGTATTGAGAAACCT+TGG 0.549573 8.1:+25445145 None:intergenic
AGCCTTTGAAAATGGTGCCA+AGG 0.550882 8.1:-25445162 MS.gene60208:CDS
ATTCTCTTTCAATCCATCCA+TGG 0.553449 8.1:+25444341 None:intergenic
CGTTTACACGGGAAAGGAAA+CGG 0.559586 8.1:-25445472 MS.gene60208:CDS
TCAAGACATTGTGTGATCCA+TGG 0.559702 8.1:-25444358 MS.gene60208:CDS
GTAAGTTGAACGATAACCAA+TGG 0.564502 8.1:+25445295 None:intergenic
TAATGAGTCTTTCAAAGTGG+TGG 0.571832 8.1:+25445531 None:intergenic
AAACACCAAGCGCGGTTGGT+TGG 0.571976 8.1:+25444855 None:intergenic
CGTTGATTGGAGGCGTCGTG+TGG 0.572282 8.1:-25444831 MS.gene60208:CDS
GGGAGTGTGTAATCAACTTG+TGG 0.575329 8.1:+25445331 None:intergenic
GCCGGTCGTTTACACGGGAA+AGG 0.576269 8.1:-25445478 MS.gene60208:CDS
TGCACTTCTCCAAATATGAC+CGG 0.576981 8.1:+25444904 None:intergenic
TTATCCGAATGGGATCAGAT+TGG 0.580343 8.1:-25445628 MS.gene60208:CDS
AGTTGTAGGGAATGGTGAGA+AGG 0.580345 8.1:-25444603 MS.gene60208:CDS
TTCAAGTAGGATAAGGGAAG+CGG 0.586112 8.1:-25444711 MS.gene60208:CDS
CCTTAGGGTATGCTTCAAGT+AGG 0.589064 8.1:-25444724 MS.gene60208:CDS
TGGTTCCATTGGTCTCACAT+AGG 0.592926 8.1:+25445672 None:intergenic
GTAATGGTCGGGGTTATTCG+AGG 0.595762 8.1:-25444940 MS.gene60208:CDS
TTGGAATCCTAATTTAGGAG+TGG 0.605814 8.1:-25444570 MS.gene60208:CDS
AGTTGGAATAAACCTAGTAA+TGG 0.606296 8.1:-25444956 MS.gene60208:CDS
CAAGTGGATAAAACGGCACA+AGG 0.608604 8.1:+25445500 None:intergenic
GACATTGTGTGATCCATGGA+TGG 0.610519 8.1:-25444354 MS.gene60208:CDS
ATGATGTATATGTTAGGTTG+GGG 0.614050 8.1:-25444676 MS.gene60208:CDS
TCAACATCAGTAGCATTCAA+AGG 0.614083 8.1:-25445700 MS.gene60208:CDS
ACTGATGTTGATGTTGAAGA+TGG 0.622854 8.1:+25445712 None:intergenic
GCTTAATGAGTCTTTCAAAG+TGG 0.631806 8.1:+25445528 None:intergenic
AGAAGTGCATGTAAAGCAAG+AGG 0.632435 8.1:-25444890 MS.gene60208:CDS
AATAAACCTAGTAATGGTCG+GGG 0.632749 8.1:-25444950 MS.gene60208:CDS
GGTAAACGACCGCACCATGT+TGG 0.632845 8.1:+25445652 None:intergenic
GAGTGGGCTCGGATTGCATG+TGG 0.634043 8.1:-25445187 MS.gene60208:CDS
TTGTTCCTATGTGAGACCAA+TGG 0.634046 8.1:-25445677 MS.gene60208:CDS
TTGTAGGGAATGGTGAGAAG+GGG 0.646958 8.1:-25444601 MS.gene60208:CDS
AGTTGATTACACACTCCCGA+TGG 0.651878 8.1:-25445326 MS.gene60208:CDS
GAAGTGCATGTAAAGCAAGA+GGG 0.674901 8.1:-25444889 MS.gene60208:CDS
ATCAACGCAAACACCAAGCG+CGG 0.676455 8.1:+25444847 None:intergenic
CCAATGGAACCAACATGGTG+CGG 0.677814 8.1:-25445661 MS.gene60208:CDS
TAGTTCGGATAAAGTTAACG+AGG 0.728148 8.1:+25445396 None:intergenic
TGAGACCAATGGAACCAACA+TGG 0.751806 8.1:-25445666 MS.gene60208:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAGGATTCCAATAAAA+AGG + chr8.1:25445489-25445508 None:intergenic 20.0%
!!! CAACTTACAAATTTTAAATG+TGG - chr8.1:25444784-25444803 MS.gene60208:CDS 20.0%
!!! TTTTATTGGAATCCTAATTT+AGG - chr8.1:25445489-25445508 MS.gene60208:CDS 20.0%
! AAGTGAATGATGTATATGTT+AGG - chr8.1:25445382-25445401 MS.gene60208:CDS 25.0%
! AATGATGTATATGTTAGGTT+GGG - chr8.1:25445387-25445406 MS.gene60208:CDS 25.0%
! AGAAAGGAAGAAAAAAACAA+CGG - chr8.1:25445026-25445045 MS.gene60208:CDS 25.0%
! ATTCAGATAATGTTGAAGAA+AGG - chr8.1:25445010-25445029 MS.gene60208:CDS 25.0%
! TCGTAACAATGTTAATGAAT+GGG - chr8.1:25444957-25444976 MS.gene60208:CDS 25.0%
! TCTGAATTTCCTAACAATAA+AGG + chr8.1:25444997-25445016 None:intergenic 25.0%
!! AAATACCCTTTTGGTAAATT+AGG + chr8.1:25445261-25445280 None:intergenic 25.0%
!! CAATTTATGGACTTGATTTT+GGG - chr8.1:25445535-25445554 MS.gene60208:CDS 25.0%
!! TTAACATTGTTACGACATTT+TGG + chr8.1:25444952-25444971 None:intergenic 25.0%
!!! AATTTACCAAAAGGGTATTT+TGG - chr8.1:25445261-25445280 MS.gene60208:CDS 25.0%
!!! ATTTACCAAAAGGGTATTTT+GGG - chr8.1:25445262-25445281 MS.gene60208:CDS 25.0%
AATATAGAACCATCATCATC+GGG + chr8.1:25445633-25445652 None:intergenic 30.0%
AGTTGGAATAAACCTAGTAA+TGG - chr8.1:25445108-25445127 MS.gene60208:CDS 30.0%
ATATAATGTCCAAACCCTTA+GGG - chr8.1:25445325-25445344 MS.gene60208:CDS 30.0%
ATGTTTGTATTGAGAAACCT+TGG + chr8.1:25444922-25444941 None:intergenic 30.0%
CAATATAGAACCATCATCAT+CGG + chr8.1:25445634-25445653 None:intergenic 30.0%
CCAAAATCAAGTCCATAAAT+TGG + chr8.1:25445537-25445556 None:intergenic 30.0%
CTTGATAAAATGCTCCTTAA+AGG + chr8.1:25445691-25445710 None:intergenic 30.0%
GAATGATGTATATGTTAGGT+TGG - chr8.1:25445386-25445405 MS.gene60208:CDS 30.0%
GATCAACTTCCTTTATTGTT+AGG - chr8.1:25444985-25445004 MS.gene60208:CDS 30.0%
GTCGTAACAATGTTAATGAA+TGG - chr8.1:25444956-25444975 MS.gene60208:CDS 30.0%
TAACTGTAATATTCCGAAGA+AGG + chr8.1:25444706-25444725 None:intergenic 30.0%
TGGAACCTAATTTACCAAAA+GGG - chr8.1:25445253-25445272 MS.gene60208:CDS 30.0%
TTTATTCCAACTCTCATTTG+CGG + chr8.1:25445100-25445119 None:intergenic 30.0%
! AACAATAAAGGAAGTTGATC+AGG + chr8.1:25444985-25445004 None:intergenic 30.0%
! ATGATGTATATGTTAGGTTG+GGG - chr8.1:25445388-25445407 MS.gene60208:CDS 30.0%
! CCAATTTATGGACTTGATTT+TGG - chr8.1:25445534-25445553 MS.gene60208:CDS 30.0%
!!! GTTGCATTTCATTTTTGAGT+GGG - chr8.1:25444861-25444880 MS.gene60208:CDS 30.0%
AAGGAAGAAAAAAACAACGG+TGG - chr8.1:25445029-25445048 MS.gene60208:CDS 35.0%
AATAAACCTAGTAATGGTCG+GGG - chr8.1:25445114-25445133 MS.gene60208:CDS 35.0%
ACCAATGGAATATCTTCCAT+CGG + chr8.1:25444757-25444776 None:intergenic 35.0%
ACTGATGTTGATGTTGAAGA+TGG + chr8.1:25444355-25444374 None:intergenic 35.0%
AGTAGCCAAAACATCCAAAA+TGG + chr8.1:25445292-25445311 None:intergenic 35.0%
CAACTAACCACTCCTAAATT+AGG + chr8.1:25445504-25445523 None:intergenic 35.0%
CTAATTTAGGAGTGGTTAGT+TGG - chr8.1:25445502-25445521 MS.gene60208:CDS 35.0%
GAATAAACCTAGTAATGGTC+GGG - chr8.1:25445113-25445132 MS.gene60208:CDS 35.0%
GATATAATGTCCAAACCCTT+AGG - chr8.1:25445324-25445343 MS.gene60208:CDS 35.0%
GCTTAATGAGTCTTTCAAAG+TGG + chr8.1:25444539-25444558 None:intergenic 35.0%
GGAATAAACCTAGTAATGGT+CGG - chr8.1:25445112-25445131 MS.gene60208:CDS 35.0%
GTATGCTTCAAGTAGGATAA+GGG - chr8.1:25445347-25445366 MS.gene60208:CDS 35.0%
GTGGAACCTAATTTACCAAA+AGG - chr8.1:25445252-25445271 MS.gene60208:CDS 35.0%
TAATGAGTCTTTCAAAGTGG+TGG + chr8.1:25444536-25444555 None:intergenic 35.0%
TAGTTCGGATAAAGTTAACG+AGG + chr8.1:25444671-25444690 None:intergenic 35.0%
TCAACATCAGTAGCATTCAA+AGG - chr8.1:25444364-25444383 MS.gene60208:CDS 35.0%
TCGTTAACTTTATCCGAACT+AGG - chr8.1:25444670-25444689 MS.gene60208:CDS 35.0%
TCGTTTACCATTATCCGAAT+GGG - chr8.1:25444426-25444445 MS.gene60208:CDS 35.0%
TGGTTGTCATTGCCAATTTA+TGG - chr8.1:25445522-25445541 MS.gene60208:CDS 35.0%
TGTAATCAACTTGTGGGATA+AGG + chr8.1:25444729-25444748 None:intergenic 35.0%
TTGGAATCCTAATTTAGGAG+TGG - chr8.1:25445494-25445513 MS.gene60208:CDS 35.0%
! GGGTGGTAAAAGTATATTGT+TGG + chr8.1:25444478-25444497 None:intergenic 35.0%
! GTAAGTTGAACGATAACCAA+TGG + chr8.1:25444772-25444791 None:intergenic 35.0%
! TTTATGGACTTGATTTTGGG+TGG - chr8.1:25445538-25445557 MS.gene60208:CDS 35.0%
!! CGATGATGATGGTTCTATAT+TGG - chr8.1:25445632-25445651 MS.gene60208:CDS 35.0%
!! CGTTGCATTTCATTTTTGAG+TGG - chr8.1:25444860-25444879 MS.gene60208:CDS 35.0%
!! GTATTTTGGGAATGCCATTT+TGG - chr8.1:25445275-25445294 MS.gene60208:CDS 35.0%
!! TCCAAGATCTTCAAGTTGTA+GGG - chr8.1:25445448-25445467 MS.gene60208:CDS 35.0%
!! TTATGGACTTGATTTTGGGT+GGG - chr8.1:25445539-25445558 MS.gene60208:CDS 35.0%
!! TTCCAAGATCTTCAAGTTGT+AGG - chr8.1:25445447-25445466 MS.gene60208:CDS 35.0%
!!! ATTTCATTTTTGAGTGGGCT+CGG - chr8.1:25444866-25444885 MS.gene60208:CDS 35.0%
!!! GAATGCCATTTTGGATGTTT+TGG - chr8.1:25445284-25445303 MS.gene60208:CDS 35.0%
AAAATGAAATGCAACGCGCT+TGG + chr8.1:25444856-25444875 None:intergenic 40.0%
AAGCAGTAACCGGTCATATT+TGG - chr8.1:25445151-25445170 MS.gene60208:CDS 40.0%
AGGTGAAAAGTCACCTAGTT+CGG + chr8.1:25444686-25444705 None:intergenic 40.0%
ATCTGATCCCATTCGGATAA+TGG + chr8.1:25444436-25444455 None:intergenic 40.0%
CCAATGGAATATCTTCCATC+GGG + chr8.1:25444756-25444775 None:intergenic 40.0%
CTACTTGAAGCATACCCTAA+GGG + chr8.1:25445342-25445361 None:intergenic 40.0%
GAAAAACCGCAAATGAGAGT+TGG - chr8.1:25445091-25445110 MS.gene60208:CDS 40.0%
GAAGTGCATGTAAAGCAAGA+GGG - chr8.1:25445175-25445194 MS.gene60208:CDS 40.0%
GATCCATATGGATGCCTTTA+AGG - chr8.1:25445674-25445693 MS.gene60208:CDS 40.0%
GATCTCGAATGCAACTCATT+GGG - chr8.1:25444622-25444641 MS.gene60208:CDS 40.0%
GATTCCAATCTGATCCCATT+CGG + chr8.1:25444443-25444462 None:intergenic 40.0%
GGAACACATGAAGAACTTGA+TGG - chr8.1:25445585-25445604 MS.gene60208:CDS 40.0%
GGAGTGTGTAATCAACTTGT+GGG + chr8.1:25444735-25444754 None:intergenic 40.0%
GGTATGCTTCAAGTAGGATA+AGG - chr8.1:25445346-25445365 MS.gene60208:CDS 40.0%
GTCGTTTACCATTATCCGAA+TGG - chr8.1:25444425-25444444 MS.gene60208:CDS 40.0%
TCAAGACATTGTGTGATCCA+TGG - chr8.1:25445706-25445725 MS.gene60208:CDS 40.0%
TCCCTACAACTTGAAGATCT+TGG + chr8.1:25445452-25445471 None:intergenic 40.0%
TGATCTCGAATGCAACTCAT+TGG - chr8.1:25444621-25444640 MS.gene60208:CDS 40.0%
TGCACTTCTCCAAATATGAC+CGG + chr8.1:25445163-25445182 None:intergenic 40.0%
TTATCCGAATGGGATCAGAT+TGG - chr8.1:25444436-25444455 MS.gene60208:CDS 40.0%
TTCAAGTAGGATAAGGGAAG+CGG - chr8.1:25445353-25445372 MS.gene60208:CDS 40.0%
TTGTCTGCAAGAGATCCATA+TGG - chr8.1:25445662-25445681 MS.gene60208:CDS 40.0%
TTGTTCCTATGTGAGACCAA+TGG - chr8.1:25444387-25444406 MS.gene60208:CDS 40.0%
! AACCTTGGCACCATTTTCAA+AGG + chr8.1:25444907-25444926 None:intergenic 40.0%
! AGAAGTGCATGTAAAGCAAG+AGG - chr8.1:25445174-25445193 MS.gene60208:CDS 40.0%
! AGGTGACTTTTCACCTTCTT+CGG - chr8.1:25444690-25444709 MS.gene60208:CDS 40.0%
! ATGTGGTGAGCCTTTGAAAA+TGG - chr8.1:25444894-25444913 MS.gene60208:CDS 40.0%
! GCATTCCCAAAATACCCTTT+TGG + chr8.1:25445270-25445289 None:intergenic 40.0%
!! AAAGTGGTGGCGATTTTAGT+GGG + chr8.1:25444523-25444542 None:intergenic 40.0%
!! GATCTTCAAGTTGTAGGGAA+TGG - chr8.1:25445453-25445472 MS.gene60208:CDS 40.0%
!! TATGGACTTGATTTTGGGTG+GGG - chr8.1:25445540-25445559 MS.gene60208:CDS 40.0%
!!! TGTTTTGGCTACTAGTCTAG+CGG - chr8.1:25445299-25445318 MS.gene60208:CDS 40.0%
AAGGAGGTTTATATGGGGCT+AGG - chr8.1:25445564-25445583 MS.gene60208:CDS 45.0%
AGTTGATTACACACTCCCGA+TGG - chr8.1:25444738-25444757 MS.gene60208:CDS 45.0%
AGTTGTAGGGAATGGTGAGA+AGG - chr8.1:25445461-25445480 MS.gene60208:CDS 45.0%
ATCTCACATGCAGTTGCAGA+TGG - chr8.1:25444829-25444848 MS.gene60208:CDS 45.0%
CAAGTGGATAAAACGGCACA+AGG + chr8.1:25444567-25444586 None:intergenic 45.0%
CCCGATGGAAGATATTCCAT+TGG - chr8.1:25444753-25444772 MS.gene60208:CDS 45.0%
CCTACTTGAAGCATACCCTA+AGG + chr8.1:25445343-25445362 None:intergenic 45.0%
CCTTAGGGTATGCTTCAAGT+AGG - chr8.1:25445340-25445359 MS.gene60208:CDS 45.0%
CGTTTACACGGGAAAGGAAA+CGG - chr8.1:25444592-25444611 MS.gene60208:CDS 45.0%
GAATAACCCCGACCATTACT+AGG + chr8.1:25445123-25445142 None:intergenic 45.0%
GACATTGTGTGATCCATGGA+TGG - chr8.1:25445710-25445729 MS.gene60208:CDS 45.0%
GTTGTAGGGAATGGTGAGAA+GGG - chr8.1:25445462-25445481 MS.gene60208:CDS 45.0%
TCGAGGTATGAAGCAGTAAC+CGG - chr8.1:25445141-25445160 MS.gene60208:CDS 45.0%
TGAAGCATACCCTAAGGGTT+TGG + chr8.1:25445337-25445356 None:intergenic 45.0%
TGAGACCAATGGAACCAACA+TGG - chr8.1:25444398-25444417 MS.gene60208:CDS 45.0%
TGGGGGAAGGAGGTTTATAT+GGG - chr8.1:25445558-25445577 MS.gene60208:CDS 45.0%
TTGTAGGGAATGGTGAGAAG+GGG - chr8.1:25445463-25445482 MS.gene60208:CDS 45.0%
! AGCCTTTGAAAATGGTGCCA+AGG - chr8.1:25444902-25444921 MS.gene60208:CDS 45.0%
! GGGAGTGTGTAATCAACTTG+TGG + chr8.1:25444736-25444755 None:intergenic 45.0%
! GTGAGAAGGGGCCTTTTTAT+TGG - chr8.1:25445475-25445494 MS.gene60208:CDS 45.0%
! TGGTTCCATTGGTCTCACAT+AGG + chr8.1:25444395-25444414 None:intergenic 45.0%
!! AAGTGGTGGCGATTTTAGTG+GGG + chr8.1:25444522-25444541 None:intergenic 45.0%
!! ATGGACTTGATTTTGGGTGG+GGG - chr8.1:25445541-25445560 MS.gene60208:CDS 45.0%
!! CAAAGTGGTGGCGATTTTAG+TGG + chr8.1:25444524-25444543 None:intergenic 45.0%
!! GCTCCTTAAAGGCATCCATA+TGG + chr8.1:25445680-25445699 None:intergenic 45.0%
!! TTGGTGTTTGCGTTGATTGG+AGG - chr8.1:25445223-25445242 MS.gene60208:CDS 45.0%
!!! GTTTTGGCTACTAGTCTAGC+GGG - chr8.1:25445300-25445319 MS.gene60208:CDS 45.0%
ATCAACGCAAACACCAAGCG+CGG + chr8.1:25445220-25445239 None:intergenic 50.0%
CCAATGGAACCAACATGGTG+CGG - chr8.1:25444403-25444422 MS.gene60208:CDS 50.0%
CGACCGGCAAGTGGATAAAA+CGG + chr8.1:25444574-25444593 None:intergenic 50.0%
CTTCCTAGTCCCGATGATGA+TGG - chr8.1:25445621-25445640 MS.gene60208:CDS 50.0%
GAACCATCATCATCGGGACT+AGG + chr8.1:25445627-25445646 None:intergenic 50.0%
GGGGGAAGGAGGTTTATATG+GGG - chr8.1:25445559-25445578 MS.gene60208:CDS 50.0%
GTAATGGTCGGGGTTATTCG+AGG - chr8.1:25445124-25445143 MS.gene60208:CDS 50.0%
GTGGGGGAAGGAGGTTTATA+TGG - chr8.1:25445557-25445576 MS.gene60208:CDS 50.0%
TCCTTTCCCGTGTAAACGAC+CGG + chr8.1:25444590-25444609 None:intergenic 50.0%
TTTACCACCCCCCAAAAAGC+TGG - chr8.1:25444488-25444507 MS.gene60208:CDS 50.0%
! GAGGTAAGCCAGCTTTTTGG+GGG + chr8.1:25444499-25444518 None:intergenic 50.0%
! GGAGGTAAGCCAGCTTTTTG+GGG + chr8.1:25444500-25444519 None:intergenic 50.0%
! GGGAGGTAAGCCAGCTTTTT+GGG + chr8.1:25444501-25444520 None:intergenic 50.0%
!! ACTTGATTTTGGGTGGGGGA+AGG - chr8.1:25445545-25445564 MS.gene60208:CDS 50.0%
!! AGGTAAGCCAGCTTTTTGGG+GGG + chr8.1:25444498-25444517 None:intergenic 50.0%
!! AGTGGTGGCGATTTTAGTGG+GGG + chr8.1:25444521-25444540 None:intergenic 50.0%
!! CGCTTGGTGTTTGCGTTGAT+TGG - chr8.1:25445220-25445239 MS.gene60208:CDS 50.0%
!!! GTGCCGTTTTATCCACTTGC+CGG - chr8.1:25444568-25444587 MS.gene60208:CDS 50.0%
AAACACCAAGCGCGGTTGGT+TGG + chr8.1:25445212-25445231 None:intergenic 55.0%
AATGACCAACCAACCGCGCT+TGG - chr8.1:25445204-25445223 MS.gene60208:CDS 55.0%
CACTTGCCGGTCGTTTACAC+GGG - chr8.1:25444581-25444600 MS.gene60208:CDS 55.0%
CCACTTGCCGGTCGTTTACA+CGG - chr8.1:25444580-25444599 MS.gene60208:CDS 55.0%
GGTAAACGACCGCACCATGT+TGG + chr8.1:25444415-25444434 None:intergenic 55.0%
! GGGGAGGTAAGCCAGCTTTT+TGG + chr8.1:25444502-25444521 None:intergenic 55.0%
!! CCGCACCATGTTGGTTCCAT+TGG + chr8.1:25444406-25444425 None:intergenic 55.0%
!! TAAGCCAGCTTTTTGGGGGG+TGG + chr8.1:25444495-25444514 None:intergenic 55.0%
!! TGATTTTGGGTGGGGGAAGG+AGG - chr8.1:25445548-25445567 MS.gene60208:CDS 55.0%
ACGCAAACACCAAGCGCGGT+TGG + chr8.1:25445216-25445235 None:intergenic 60.0%
CCGTGTAAACGACCGGCAAG+TGG + chr8.1:25444583-25444602 None:intergenic 60.0%
GCCGGTCGTTTACACGGGAA+AGG - chr8.1:25444586-25444605 MS.gene60208:CDS 60.0%
! GAGTGGGCTCGGATTGCATG+TGG - chr8.1:25444877-25444896 MS.gene60208:CDS 60.0%
!! CGTTGATTGGAGGCGTCGTG+TGG - chr8.1:25445233-25445252 MS.gene60208:CDS 60.0%
!! GGTGGCGATTTTAGTGGGGG+AGG + chr8.1:25444518-25444537 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25444349 25445737 25444349 ID=MS.gene60208
chr8.1 mRNA 25444349 25445737 25444349 ID=MS.gene60208.t1;Parent=MS.gene60208
chr8.1 exon 25444349 25445737 25444349 ID=MS.gene60208.t1.exon1;Parent=MS.gene60208.t1
chr8.1 CDS 25444349 25445737 25444349 ID=cds.MS.gene60208.t1;Parent=MS.gene60208.t1
Gene Sequence

>MS.gene60208

ATGCCATCTTCAACATCAACATCAGTAGCATTCAAAGGTTGTTCCTATGTGAGACCAATGGAACCAACATGGTGCGGTCGTTTACCATTATCCGAATGGGATCAGATTGGAATCATAGCACACGTGCCAACAATATACTTTTACCACCCCCCAAAAAGCTGGCTTACCTCCCCCACTAAAATCGCCACCACTTTGAAAGACTCATTAAGCAAAGTCCTTGTGCCGTTTTATCCACTTGCCGGTCGTTTACACGGGAAAGGAAACGGTCGTTTTGATCTCGAATGCAACTCATTGGGTGTTCAATTCATCGAAGCACAATCCTCGTTAACTTTATCCGAACTAGGTGACTTTTCACCTTCTTCGGAATATTACAGTTACCTTATCCCACAAGTTGATTACACACTCCCGATGGAAGATATTCCATTGGTTATCGTTCAACTTACAAATTTTAAATGTGGTGTAGTTAGTATTAGTTTGTTAATCTCACATGCAGTTGCAGATGGACCAAGCGCGTTGCATTTCATTTTTGAGTGGGCTCGGATTGCATGTGGTGAGCCTTTGAAAATGGTGCCAAGGTTTCTCAATACAAACATGTTGTCGCCAAAATGTCGTAACAATGTTAATGAATGGGAGCCTGATCAACTTCCTTTATTGTTAGGAAATTCAGATAATGTTGAAGAAAGGAAGAAAAAAACAACGGTGGCTATAATCAACTTGAGCAAAACACAAGTTGAAAAGTTGAGAAAAACCGCAAATGAGAGTTGGAATAAACCTAGTAATGGTCGGGGTTATTCGAGGTATGAAGCAGTAACCGGTCATATTTGGAGAAGTGCATGTAAAGCAAGAGGGCTTGAAAATGACCAACCAACCGCGCTTGGTGTTTGCGTTGATTGGAGGCGTCGTGTGGAACCTAATTTACCAAAAGGGTATTTTGGGAATGCCATTTTGGATGTTTTGGCTACTAGTCTAGCGGGTGATATAATGTCCAAACCCTTAGGGTATGCTTCAAGTAGGATAAGGGAAGCGGTTGAGAAAGTGAATGATGTATATGTTAGGTTGGGGATTGAGTACTTTAAGAAGCAAGAAGATTTGACTAAGTTCCAAGATCTTCAAGTTGTAGGGAATGGTGAGAAGGGGCCTTTTTATTGGAATCCTAATTTAGGAGTGGTTAGTTGGTTGTCATTGCCAATTTATGGACTTGATTTTGGGTGGGGGAAGGAGGTTTATATGGGGCTAGGAACACATGAAGAACTTGATGGAGACTCATTGCTTCTTCCTAGTCCCGATGATGATGGTTCTATATTGGTTATTATTTGTCTGCAAGAGATCCATATGGATGCCTTTAAGGAGCATTTTATCAAGACATTGTGTGATCCATGGATGGATTGA
Protein sequence

>MS.gene60208.t1

MPSSTSTSVAFKGCSYVRPMEPTWCGRLPLSEWDQIGIIAHVPTIYFYHPPKSWLTSPTKIATTLKDSLSKVLVPFYPLAGRLHGKGNGRFDLECNSLGVQFIEAQSSLTLSELGDFSPSSEYYSYLIPQVDYTLPMEDIPLVIVQLTNFKCGVVSISLLISHAVADGPSALHFIFEWARIACGEPLKMVPRFLNTNMLSPKCRNNVNEWEPDQLPLLLGNSDNVEERKKKTTVAIINLSKTQVEKLRKTANESWNKPSNGRGYSRYEAVTGHIWRSACKARGLENDQPTALGVCVDWRRRVEPNLPKGYFGNAILDVLATSLAGDIMSKPLGYASSRIREAVEKVNDVYVRLGIEYFKKQEDLTKFQDLQVVGNGEKGPFYWNPNLGVVSWLSLPIYGLDFGWGKEVYMGLGTHEELDGDSLLLPSPDDDGSILVIICLQEIHMDAFKEHFIKTLCDPWMD