Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63532.t1 | XP_003598276.1 | 91.3 | 526 | 38 | 1 | 1 | 518 | 1 | 526 | 1.70E-278 | 968 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63532.t1 | Q9SUC6 | 54.3 | 523 | 219 | 8 | 8 | 514 | 11 | 529 | 1.8e-162 | 573.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63532.t1 | G7J0C2 | 91.3 | 526 | 38 | 1 | 1 | 518 | 1 | 526 | 1.3e-278 | 968.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63532 | MS.gene028347 | PPI |
| MS.gene63532 | MS.gene36437 | PPI |
| MS.gene63532 | MS.gene028357 | PPI |
| MS.gene63532 | MS.gene28717 | PPI |
| MS.gene63532 | MS.gene36439 | PPI |
| MS.gene63532 | MS.gene28727 | PPI |
| MS.gene63532 | MS.gene36435 | PPI |
| MS.gene63532 | MS.gene35103 | PPI |
| MS.gene028369 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene35113 | PPI |
| MS.gene63532 | MS.gene028346 | PPI |
| MS.gene63532 | MS.gene028352 | PPI |
| MS.gene63532 | MS.gene28725 | PPI |
| MS.gene35104 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene36445 | PPI |
| MS.gene35105 | MS.gene63532 | PPI |
| MS.gene36438 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene28723 | PPI |
| MS.gene63532 | MS.gene35083 | PPI |
| MS.gene003357 | MS.gene63532 | PPI |
| MS.gene28724 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene047977 | PPI |
| MS.gene63532 | MS.gene35102 | PPI |
| MS.gene63532 | MS.gene28742 | PPI |
| MS.gene02146 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene28726 | PPI |
| MS.gene63532 | MS.gene35106 | PPI |
| MS.gene63532 | MS.gene028353 | PPI |
| MS.gene63532 | MS.gene36444 | PPI |
| MS.gene63532 | MS.gene35114 | PPI |
| MS.gene36421 | MS.gene63532 | PPI |
| MS.gene63532 | MS.gene028354 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63532.t1 | MTR_3g009760 | 91.255 | 526 | 38 | 1 | 1 | 518 | 1 | 526 | 0.0 | 972 |
| MS.gene63532.t1 | MTR_3g009820 | 91.255 | 526 | 38 | 1 | 1 | 518 | 1 | 526 | 0.0 | 971 |
| MS.gene63532.t1 | MTR_3g009880 | 84.952 | 525 | 69 | 2 | 1 | 517 | 1 | 523 | 0.0 | 894 |
| MS.gene63532.t1 | MTR_2g031420 | 55.114 | 528 | 217 | 9 | 1 | 515 | 8 | 528 | 0.0 | 553 |
| MS.gene63532.t1 | MTR_2g031460 | 53.992 | 526 | 218 | 9 | 1 | 511 | 8 | 524 | 0.0 | 547 |
| MS.gene63532.t1 | MTR_4g094488 | 53.195 | 532 | 224 | 8 | 3 | 512 | 7 | 535 | 0.0 | 546 |
| MS.gene63532.t1 | MTR_2g031390 | 51.272 | 511 | 230 | 7 | 19 | 514 | 23 | 529 | 0.0 | 544 |
| MS.gene63532.t1 | MTR_4g094492 | 53.818 | 537 | 222 | 9 | 2 | 516 | 6 | 538 | 0.0 | 541 |
| MS.gene63532.t1 | MTR_4g094478 | 51.992 | 527 | 235 | 6 | 4 | 514 | 11 | 535 | 0.0 | 538 |
| MS.gene63532.t1 | MTR_4g094495 | 52.897 | 535 | 226 | 7 | 2 | 512 | 6 | 538 | 0.0 | 538 |
| MS.gene63532.t1 | MTR_4g094520 | 50.568 | 528 | 242 | 9 | 1 | 514 | 10 | 532 | 0.0 | 533 |
| MS.gene63532.t1 | MTR_4g094518 | 53.937 | 508 | 213 | 9 | 23 | 514 | 28 | 530 | 0.0 | 527 |
| MS.gene63532.t1 | MTR_4g094480 | 50.377 | 530 | 242 | 8 | 2 | 512 | 6 | 533 | 0.0 | 521 |
| MS.gene63532.t1 | MTR_4g094418 | 56.651 | 436 | 171 | 5 | 98 | 515 | 1 | 436 | 5.56e-179 | 517 |
| MS.gene63532.t1 | MTR_2g031560 | 48.092 | 524 | 256 | 6 | 4 | 514 | 3 | 523 | 6.97e-179 | 514 |
| MS.gene63532.t1 | MTR_4g094498 | 49.623 | 530 | 242 | 7 | 7 | 516 | 12 | 536 | 2.16e-177 | 510 |
| MS.gene63532.t1 | MTR_2g031590 | 47.842 | 533 | 254 | 10 | 1 | 514 | 5 | 532 | 9.90e-174 | 501 |
| MS.gene63532.t1 | MTR_2g031510 | 46.199 | 513 | 254 | 8 | 19 | 514 | 33 | 540 | 1.34e-160 | 468 |
| MS.gene63532.t1 | MTR_2g031490 | 44.195 | 534 | 274 | 9 | 1 | 514 | 9 | 538 | 7.23e-159 | 463 |
| MS.gene63532.t1 | MTR_4g091200 | 46.008 | 526 | 262 | 8 | 1 | 512 | 7 | 524 | 1.03e-156 | 457 |
| MS.gene63532.t1 | MTR_4g057990 | 44.253 | 522 | 276 | 5 | 1 | 512 | 4 | 520 | 6.92e-155 | 452 |
| MS.gene63532.t1 | MTR_6g017205 | 45.611 | 524 | 259 | 9 | 7 | 512 | 9 | 524 | 8.98e-154 | 450 |
| MS.gene63532.t1 | MTR_4g091190 | 45.541 | 527 | 257 | 9 | 7 | 512 | 9 | 526 | 1.67e-153 | 449 |
| MS.gene63532.t1 | MTR_4g091150 | 47.937 | 509 | 242 | 10 | 24 | 514 | 37 | 540 | 4.02e-153 | 448 |
| MS.gene63532.t1 | MTR_4g094515 | 46.168 | 535 | 265 | 10 | 1 | 516 | 622 | 1152 | 5.05e-153 | 466 |
| MS.gene63532.t1 | MTR_4g094515 | 45.577 | 520 | 261 | 9 | 11 | 512 | 23 | 538 | 2.27e-148 | 454 |
| MS.gene63532.t1 | MTR_4g088355 | 46.004 | 513 | 249 | 8 | 24 | 516 | 31 | 535 | 7.29e-152 | 445 |
| MS.gene63532.t1 | MTR_3g009850 | 87.069 | 232 | 30 | 0 | 287 | 518 | 1 | 232 | 3.72e-150 | 429 |
| MS.gene63532.t1 | MTR_4g091170 | 46.460 | 452 | 212 | 6 | 73 | 512 | 107 | 540 | 2.44e-144 | 427 |
| MS.gene63532.t1 | MTR_2g023590 | 46.522 | 460 | 230 | 6 | 70 | 516 | 12 | 468 | 1.51e-138 | 409 |
| MS.gene63532.t1 | MTR_1g076830 | 42.737 | 475 | 253 | 8 | 54 | 511 | 60 | 532 | 8.90e-123 | 371 |
| MS.gene63532.t1 | MTR_2g031600 | 45.935 | 246 | 115 | 6 | 1 | 231 | 1 | 243 | 5.09e-62 | 209 |
| MS.gene63532.t1 | MTR_2g031600 | 54.430 | 158 | 69 | 2 | 358 | 514 | 245 | 400 | 1.23e-53 | 186 |
| MS.gene63532.t1 | MTR_2g084370 | 72.816 | 103 | 14 | 1 | 122 | 224 | 1 | 89 | 1.54e-44 | 152 |
| MS.gene63532.t1 | MTR_2g084405 | 75.294 | 85 | 11 | 2 | 122 | 196 | 1 | 85 | 1.90e-28 | 109 |
| MS.gene63532.t1 | MTR_8g062280 | 47.458 | 59 | 29 | 1 | 460 | 518 | 21 | 77 | 2.55e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63532.t1 | AT4G20860 | 54.352 | 517 | 216 | 8 | 14 | 514 | 17 | 529 | 0.0 | 550 |
| MS.gene63532.t1 | AT5G44360 | 52.918 | 514 | 207 | 12 | 23 | 514 | 46 | 546 | 0.0 | 528 |
| MS.gene63532.t1 | AT5G44360 | 52.918 | 514 | 207 | 12 | 23 | 514 | 30 | 530 | 0.0 | 527 |
| MS.gene63532.t1 | AT5G44400 | 51.977 | 531 | 230 | 12 | 4 | 512 | 7 | 534 | 0.0 | 525 |
| MS.gene63532.t1 | AT2G34790 | 48.588 | 531 | 237 | 10 | 5 | 512 | 11 | 528 | 3.74e-180 | 517 |
| MS.gene63532.t1 | AT1G30760 | 51.373 | 510 | 223 | 11 | 20 | 512 | 29 | 530 | 1.87e-178 | 513 |
| MS.gene63532.t1 | AT1G30760 | 51.373 | 510 | 223 | 11 | 20 | 512 | 47 | 548 | 3.25e-178 | 513 |
| MS.gene63532.t1 | AT5G44390 | 51.056 | 521 | 232 | 13 | 13 | 514 | 24 | 540 | 2.39e-177 | 510 |
| MS.gene63532.t1 | AT5G44440 | 45.318 | 534 | 268 | 11 | 2 | 517 | 5 | 532 | 4.46e-163 | 473 |
| MS.gene63532.t1 | AT5G44440 | 45.318 | 534 | 268 | 11 | 2 | 517 | 75 | 602 | 5.64e-162 | 473 |
| MS.gene63532.t1 | AT5G44380 | 49.046 | 524 | 244 | 12 | 9 | 512 | 17 | 537 | 9.69e-162 | 470 |
| MS.gene63532.t1 | AT5G44380 | 49.046 | 524 | 244 | 12 | 9 | 512 | 57 | 577 | 1.62e-161 | 471 |
| MS.gene63532.t1 | AT1G26420 | 46.881 | 529 | 254 | 9 | 1 | 512 | 5 | 523 | 3.16e-160 | 466 |
| MS.gene63532.t1 | AT4G20820 | 45.455 | 517 | 259 | 10 | 8 | 512 | 19 | 524 | 6.07e-160 | 465 |
| MS.gene63532.t1 | AT1G26410 | 49.791 | 478 | 221 | 7 | 48 | 512 | 76 | 547 | 2.45e-157 | 459 |
| MS.gene63532.t1 | AT1G26380 | 46.415 | 530 | 259 | 8 | 2 | 514 | 4 | 525 | 8.09e-157 | 457 |
| MS.gene63532.t1 | AT1G26390 | 48.852 | 479 | 226 | 7 | 48 | 512 | 52 | 525 | 5.96e-155 | 452 |
| MS.gene63532.t1 | AT5G44410 | 45.714 | 525 | 259 | 10 | 7 | 514 | 14 | 529 | 9.11e-155 | 452 |
| MS.gene63532.t1 | AT1G26400 | 46.124 | 516 | 262 | 7 | 8 | 511 | 10 | 521 | 3.73e-152 | 445 |
| MS.gene63532.t1 | AT1G30710 | 43.667 | 529 | 273 | 10 | 4 | 516 | 11 | 530 | 5.22e-151 | 442 |
| MS.gene63532.t1 | AT1G34575 | 43.019 | 530 | 265 | 12 | 4 | 513 | 11 | 523 | 5.27e-150 | 440 |
| MS.gene63532.t1 | AT1G30700 | 46.154 | 507 | 251 | 9 | 24 | 514 | 26 | 526 | 1.80e-148 | 436 |
| MS.gene63532.t1 | AT1G30730 | 43.977 | 523 | 276 | 7 | 4 | 513 | 6 | 524 | 1.45e-146 | 431 |
| MS.gene63532.t1 | AT4G20800 | 47.752 | 467 | 226 | 7 | 60 | 512 | 61 | 523 | 1.44e-144 | 426 |
| MS.gene63532.t1 | AT1G11770 | 41.366 | 527 | 293 | 8 | 4 | 518 | 11 | 533 | 5.40e-144 | 425 |
| MS.gene63532.t1 | AT1G01980 | 43.403 | 523 | 272 | 10 | 15 | 518 | 21 | 538 | 9.46e-144 | 424 |
| MS.gene63532.t1 | AT4G20840 | 42.604 | 507 | 272 | 8 | 22 | 514 | 34 | 535 | 3.84e-141 | 417 |
| MS.gene63532.t1 | AT4G20830 | 43.249 | 511 | 273 | 8 | 19 | 516 | 32 | 538 | 2.52e-138 | 410 |
| MS.gene63532.t1 | AT1G30740 | 42.471 | 518 | 281 | 8 | 11 | 514 | 14 | 528 | 5.02e-138 | 409 |
| MS.gene63532.t1 | AT1G30740 | 42.471 | 518 | 281 | 8 | 11 | 514 | 17 | 531 | 7.09e-138 | 409 |
| MS.gene63532.t1 | AT1G30720 | 41.440 | 514 | 284 | 7 | 13 | 513 | 16 | 525 | 9.57e-138 | 409 |
| MS.gene63532.t1 | AT4G20830 | 43.222 | 509 | 272 | 8 | 19 | 514 | 32 | 536 | 1.72e-137 | 409 |
| MS.gene63532.t1 | AT2G34810 | 42.166 | 517 | 281 | 7 | 15 | 514 | 24 | 539 | 8.68e-134 | 399 |
Find 92 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGGTTTAGAAGGTATT+TGG | 0.185112 | 3.3:+5010442 | MS.gene63532:CDS |
| GCTCACCTTATAGATGTAAA+TGG | 0.201621 | 3.3:+5009913 | MS.gene63532:CDS |
| TCTTGCATGGAAGATAAATT+TGG | 0.222820 | 3.3:+5010017 | MS.gene63532:CDS |
| CATCCACTTTATATGCTTGT+TGG | 0.273212 | 3.3:-5010628 | None:intergenic |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.3:+5009988 | MS.gene63532:CDS |
| CGATGAATTTGCTTGGATTC+AGG | 0.286513 | 3.3:+5009042 | MS.gene63532:CDS |
| AAGTGGATGAGAATGCTTTA+TGG | 0.287403 | 3.3:+5010642 | MS.gene63532:CDS |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.3:+5010697 | MS.gene63532:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.3:-5008877 | None:intergenic |
| CTATTCAGCAAGAGTTCTAT+AGG | 0.301114 | 3.3:-5010357 | None:intergenic |
| CTTGATGAATTCACCCATCT+TGG | 0.307956 | 3.3:-5008827 | None:intergenic |
| TTGTCTTGCAACCCTAGTTC+AGG | 0.322720 | 3.3:-5010270 | None:intergenic |
| CACATTAGCAATGCTTATAA+TGG | 0.340644 | 3.3:+5010485 | MS.gene63532:CDS |
| AGAGAGAGTTAAACTTGGTT+TGG | 0.351945 | 3.3:-5010200 | None:intergenic |
| CACATCAGTGGAGGTGGCTT+TGG | 0.360702 | 3.3:+5009844 | MS.gene63532:CDS |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.3:-5010432 | None:intergenic |
| TCTAAAGCAAGTAAAGTTCA+TGG | 0.367121 | 3.3:+5009100 | MS.gene63532:CDS |
| GGTACTCAGAAGCAAGTGTT+TGG | 0.380263 | 3.3:+5010757 | MS.gene63532:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.3:+5009058 | MS.gene63532:CDS |
| GAGCAACCAAGCTTCTTAAT+AGG | 0.382965 | 3.3:+5010094 | MS.gene63532:CDS |
| CAACACTTGTGGATACAATA+AGG | 0.404009 | 3.3:-5008786 | None:intergenic |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.3:-5010438 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.3:+5010435 | MS.gene63532:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.3:-5010675 | None:intergenic |
| CTTGTACAACATACAATATA+TGG | 0.415255 | 3.3:+5010575 | MS.gene63532:CDS |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.3:+5010698 | MS.gene63532:CDS |
| GTACTCAGAAGCAAGTGTTT+GGG | 0.433402 | 3.3:+5010758 | MS.gene63532:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.3:-5008990 | None:intergenic |
| TCATTCAGTCTTCCACCATA+AGG | 0.436662 | 3.3:-5010510 | None:intergenic |
| TGGAGGTGGCTTTGGTACCT+TGG | 0.438981 | 3.3:+5009852 | MS.gene63532:CDS |
| AAGTTTAACTCTCTCTTCCT+TGG | 0.446047 | 3.3:+5010207 | MS.gene63532:CDS |
| ATCTTGCCGATGAATTTGCT+TGG | 0.459055 | 3.3:+5009035 | MS.gene63532:CDS |
| ATGCTTATAATGGAACCTTA+TGG | 0.459315 | 3.3:+5010495 | MS.gene63532:CDS |
| AATCAGAGTTTCCCTGAACT+AGG | 0.461387 | 3.3:+5010258 | MS.gene63532:CDS |
| CTCACCTTATAGATGTAAAT+GGG | 0.461407 | 3.3:+5009914 | MS.gene63532:CDS |
| CAAGGGAAACTTTAAGCGAT+TGG | 0.468107 | 3.3:+5010794 | MS.gene63532:CDS |
| AATTCTTGATAGAAAATCAA+TGG | 0.470027 | 3.3:+5009939 | MS.gene63532:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.3:+5010711 | MS.gene63532:CDS |
| CTTCATGCAATTTATCTGCT+AGG | 0.476079 | 3.3:-5010125 | None:intergenic |
| TGTCTTGCAACCCTAGTTCA+GGG | 0.476537 | 3.3:-5010269 | None:intergenic |
| AAGACTGCATTGAAATGAGT+TGG | 0.478707 | 3.3:+5010292 | MS.gene63532:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.3:-5008989 | None:intergenic |
| TAAGTTGTTAATTCAGGGGA+GGG | 0.480193 | 3.3:-5010886 | None:intergenic |
| TATTCAGCAAGAGTTCTATA+GGG | 0.484064 | 3.3:-5010356 | None:intergenic |
| CATTGAAACAAAATCCAAGA+TGG | 0.486669 | 3.3:+5008813 | MS.gene63532:CDS |
| TTGGGGCATCAAGTACTTCA+AGG | 0.488511 | 3.3:+5010776 | MS.gene63532:CDS |
| TTCAGAAAAGCTACTACCTA+TGG | 0.493468 | 3.3:-5008729 | None:intergenic |
| TTCAATGATTCCAACACTTG+TGG | 0.495973 | 3.3:-5008797 | None:intergenic |
| CTTATTGTATCCACAAGTGT+TGG | 0.498395 | 3.3:+5008787 | MS.gene63532:CDS |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.3:-5008876 | None:intergenic |
| AGAATAGTTGCTCAAAATAG+TGG | 0.501050 | 3.3:+5010159 | MS.gene63532:CDS |
| AAGACAGTCCTTCATAATCA+TGG | 0.503011 | 3.3:-5008952 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.3:-5010693 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.509234 | 3.3:+5010625 | MS.gene63532:CDS |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 3.3:+5009940 | MS.gene63532:CDS |
| CTGCCACCTATTAAGAAGCT+TGG | 0.525159 | 3.3:-5010100 | None:intergenic |
| GTGGCTTTGGTACCTTGGTA+AGG | 0.531498 | 3.3:+5009857 | MS.gene63532:CDS |
| GTTCAACATTCAAAGAACAT+TGG | 0.536377 | 3.3:+5010065 | MS.gene63532:CDS |
| ATGTTGAACACTGCAACAAT+AGG | 0.538107 | 3.3:-5010051 | None:intergenic |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.3:+5009112 | MS.gene63532:CDS |
| ATGCAGTGCATGTTAACCAT+AGG | 0.544066 | 3.3:+5008713 | MS.gene63532:CDS |
| ATTGAAACAAAATCCAAGAT+GGG | 0.551532 | 3.3:+5008814 | MS.gene63532:CDS |
| GGTGGTCACATCAGTGGAGG+TGG | 0.552995 | 3.3:+5009838 | MS.gene63532:intron |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.3:+5010426 | MS.gene63532:CDS |
| GAATTTGCTTGGATTCAGGC+TGG | 0.564257 | 3.3:+5009046 | MS.gene63532:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.3:-5008872 | None:intergenic |
| ATCAGAGTTTCCCTGAACTA+GGG | 0.565213 | 3.3:+5010259 | MS.gene63532:CDS |
| TTGAACACTGCAACAATAGG+TGG | 0.567104 | 3.3:-5010048 | None:intergenic |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.3:+5008926 | MS.gene63532:CDS |
| ACATACAATATATGGTCAAG+TGG | 0.577424 | 3.3:+5010583 | MS.gene63532:CDS |
| TTAAGTTGTTAATTCAGGGG+AGG | 0.578715 | 3.3:-5010887 | None:intergenic |
| AAGGAAGAGAGAGTTAAACT+TGG | 0.579262 | 3.3:-5010205 | None:intergenic |
| TGGGGCATCAAGTACTTCAA+GGG | 0.581811 | 3.3:+5010777 | MS.gene63532:CDS |
| GGTACCTTGGTAAGGAAACA+TGG | 0.585139 | 3.3:+5009865 | MS.gene63532:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.3:+5008944 | MS.gene63532:CDS |
| CTGAATCCAAGCAAATTCAT+CGG | 0.598221 | 3.3:-5009041 | None:intergenic |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.3:+5008919 | MS.gene63532:CDS |
| GCAACAAGTATGACAATACA+AGG | 0.601545 | 3.3:+5010736 | MS.gene63532:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.603811 | 3.3:+5010759 | MS.gene63532:CDS |
| CTTATAATGGAACCTTATGG+TGG | 0.606994 | 3.3:+5010498 | MS.gene63532:CDS |
| CATACAATATATGGTCAAGT+GGG | 0.609377 | 3.3:+5010584 | MS.gene63532:CDS |
| GGAGTAGGTGGTCACATCAG+TGG | 0.610281 | 3.3:+5009832 | MS.gene63532:intron |
| CAACCAAGCTTCTTAATAGG+TGG | 0.612241 | 3.3:+5010097 | MS.gene63532:CDS |
| CAAGATTAATGTCGATCGAA+CGG | 0.627262 | 3.3:-5009018 | None:intergenic |
| GCTCAAACTAATTACCTGCA+GGG | 0.638432 | 3.3:-5009126 | None:intergenic |
| CAGGGGAGGGAATTCAGAAG+AGG | 0.643159 | 3.3:-5010873 | None:intergenic |
| GAAGGTGTCATAATGAGAAG+AGG | 0.648177 | 3.3:-5008854 | None:intergenic |
| TAGACCATGTTTCCTTACCA+AGG | 0.652475 | 3.3:-5009869 | None:intergenic |
| GTAGGTGGTCACATCAGTGG+AGG | 0.669019 | 3.3:+5009835 | MS.gene63532:intron |
| ATTCAAAGAACATTGGAACA+AGG | 0.678567 | 3.3:+5010072 | MS.gene63532:CDS |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.3:+5008929 | MS.gene63532:CDS |
| CACTTGTGGATACAATAAGG+AGG | 0.699246 | 3.3:-5008783 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAACTATAACAAA+CGG | + | chr3.3:5009624-5009643 | MS.gene63532:intron | 10.0% |
| !!! | TCATAATAATTTAGTTTTTT+TGG | + | chr3.3:5009452-5009471 | MS.gene63532:intron | 10.0% |
| !!! | TTATTTTAATATGTAACAAA+CGG | - | chr3.3:5009351-5009370 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAAAAAACCGT+TGG | - | chr3.3:5009718-5009737 | None:intergenic | 15.0% |
| !!! | AAAAATTGATTTTTTGTTTG+AGG | - | chr3.3:5009544-5009563 | None:intergenic | 15.0% |
| !!! | TTTTCTTAAAAAATAACCAA+CGG | + | chr3.3:5009699-5009718 | MS.gene63532:intron | 15.0% |
| !! | AATTCTTGATAGAAAATCAA+TGG | + | chr3.3:5009939-5009958 | MS.gene63532:CDS | 20.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | + | chr3.3:5009940-5009959 | MS.gene63532:CDS | 20.0% |
| !!! | ATCTAGTTTTGACTTTATTT+TGG | + | chr3.3:5009324-5009343 | MS.gene63532:intron | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.3:5008992-5009011 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.3:5009793-5009812 | MS.gene63532:intron | 25.0% |
| ! | ATTGAAACAAAATCCAAGAT+GGG | + | chr3.3:5008814-5008833 | MS.gene63532:CDS | 25.0% |
| ! | CTTGTACAACATACAATATA+TGG | + | chr3.3:5010575-5010594 | MS.gene63532:CDS | 25.0% |
| !! | TTAAGTTATCTTTTTCTCCA+AGG | - | chr3.3:5010227-5010246 | None:intergenic | 25.0% |
| !! | TTTTCCCATTTACATCTATA+AGG | - | chr3.3:5009921-5009940 | None:intergenic | 25.0% |
| !!! | GTTTGGATTGTTTTTGAATT+TGG | - | chr3.3:5010186-5010205 | None:intergenic | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.3:5010711-5010730 | MS.gene63532:CDS | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.3:5010625-5010644 | MS.gene63532:CDS | 30.0% | |
| ACATACAATATATGGTCAAG+TGG | + | chr3.3:5010583-5010602 | MS.gene63532:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.3:5010159-5010178 | MS.gene63532:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.3:5010698-5010717 | MS.gene63532:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.3:5008902-5008921 | MS.gene63532:CDS | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.3:5010072-5010091 | MS.gene63532:CDS | 30.0% | |
| CACATTAGCAATGCTTATAA+TGG | + | chr3.3:5010485-5010504 | MS.gene63532:CDS | 30.0% | |
| CATACAATATATGGTCAAGT+GGG | + | chr3.3:5010584-5010603 | MS.gene63532:CDS | 30.0% | |
| CATTGAAACAAAATCCAAGA+TGG | + | chr3.3:5008813-5008832 | MS.gene63532:CDS | 30.0% | |
| CTCACCTTATAGATGTAAAT+GGG | + | chr3.3:5009914-5009933 | MS.gene63532:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.3:5008993-5009012 | None:intergenic | 30.0% | |
| GTTCAACATTCAAAGAACAT+TGG | + | chr3.3:5010065-5010084 | MS.gene63532:CDS | 30.0% | |
| TATTCAGCAAGAGTTCTATA+GGG | - | chr3.3:5010359-5010378 | None:intergenic | 30.0% | |
| TCTTGCATGGAAGATAAATT+TGG | + | chr3.3:5010017-5010036 | MS.gene63532:CDS | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.3:5008879-5008898 | None:intergenic | 30.0% | |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.3:5008903-5008922 | None:intergenic | 30.0% |
| ! | ATGCTTATAATGGAACCTTA+TGG | + | chr3.3:5010495-5010514 | MS.gene63532:CDS | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.3:5010844-5010863 | None:intergenic | 30.0% |
| ! | CTCTTTTACATAGTCAGATT+TGG | - | chr3.3:5010412-5010431 | None:intergenic | 30.0% |
| ! | TACAAGTTTCCTTTTCTATG+TGG | - | chr3.3:5010561-5010580 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.3:5010843-5010862 | None:intergenic | 30.0% |
| ! | TCTAAAGCAAGTAAAGTTCA+TGG | + | chr3.3:5009100-5009119 | MS.gene63532:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.3:5010554-5010573 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.3:5010555-5010574 | None:intergenic | 30.0% |
| !! | ACTTATTTCTATAAGCTCTC+TGG | + | chr3.3:5009269-5009288 | MS.gene63532:intron | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+TGG | + | chr3.3:5010318-5010337 | MS.gene63532:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.3:5009957-5009976 | MS.gene63532:CDS | 30.0% |
| AAGACAGTCCTTCATAATCA+TGG | - | chr3.3:5008955-5008974 | None:intergenic | 35.0% | |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.3:5010292-5010311 | MS.gene63532:CDS | 35.0% | |
| AAGGAAGAGAGAGTTAAACT+TGG | - | chr3.3:5010208-5010227 | None:intergenic | 35.0% | |
| AAGTGGATGAGAATGCTTTA+TGG | + | chr3.3:5010642-5010661 | MS.gene63532:CDS | 35.0% | |
| AAGTTTAACTCTCTCTTCCT+TGG | + | chr3.3:5010207-5010226 | MS.gene63532:CDS | 35.0% | |
| AGAGAGAGTTAAACTTGGTT+TGG | - | chr3.3:5010203-5010222 | None:intergenic | 35.0% | |
| ATGTTGAACACTGCAACAAT+AGG | - | chr3.3:5010054-5010073 | None:intergenic | 35.0% | |
| CAACACTTGTGGATACAATA+AGG | - | chr3.3:5008789-5008808 | None:intergenic | 35.0% | |
| CAAGATTAATGTCGATCGAA+CGG | - | chr3.3:5009021-5009040 | None:intergenic | 35.0% | |
| CATCCACTTTATATGCTTGT+TGG | - | chr3.3:5010631-5010650 | None:intergenic | 35.0% | |
| CTATTCAGCAAGAGTTCTAT+AGG | - | chr3.3:5010360-5010379 | None:intergenic | 35.0% | |
| CTGAATCCAAGCAAATTCAT+CGG | - | chr3.3:5009044-5009063 | None:intergenic | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.3:5008880-5008899 | None:intergenic | 35.0% | |
| CTTATTGTATCCACAAGTGT+TGG | + | chr3.3:5008787-5008806 | MS.gene63532:CDS | 35.0% | |
| CTTCATGCAATTTATCTGCT+AGG | - | chr3.3:5010128-5010147 | None:intergenic | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.3:5010697-5010716 | MS.gene63532:CDS | 35.0% | |
| GCAACAAGTATGACAATACA+AGG | + | chr3.3:5010736-5010755 | MS.gene63532:CDS | 35.0% | |
| GCTCACCTTATAGATGTAAA+TGG | + | chr3.3:5009913-5009932 | MS.gene63532:CDS | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.3:5010696-5010715 | None:intergenic | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.3:5008875-5008894 | None:intergenic | 35.0% | |
| TTCAATGATTCCAACACTTG+TGG | - | chr3.3:5008800-5008819 | None:intergenic | 35.0% | |
| TTCAGAAAAGCTACTACCTA+TGG | - | chr3.3:5008732-5008751 | None:intergenic | 35.0% | |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.3:5010549-5010568 | MS.gene63532:CDS | 35.0% |
| ! | CTTATAATGGAACCTTATGG+TGG | + | chr3.3:5010498-5010517 | MS.gene63532:CDS | 35.0% |
| ! | GATAAACACTGTGTTTTCAC+TGG | - | chr3.3:5008756-5008775 | None:intergenic | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.3:5010845-5010864 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.3:5010442-5010461 | MS.gene63532:CDS | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.3:5010678-5010697 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.3:5010835-5010854 | MS.gene63532:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.3:5009956-5009975 | MS.gene63532:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.3:5009967-5009986 | MS.gene63532:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.3:5010004-5010023 | MS.gene63532:CDS | 35.0% |
| AATCAGAGTTTCCCTGAACT+AGG | + | chr3.3:5010258-5010277 | MS.gene63532:CDS | 40.0% | |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.3:5009811-5009830 | MS.gene63532:intron | 40.0% | |
| AGCTCAAACTAATTACCTGC+AGG | - | chr3.3:5009130-5009149 | None:intergenic | 40.0% | |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.3:5010441-5010460 | None:intergenic | 40.0% | |
| ATCAGAGTTTCCCTGAACTA+GGG | + | chr3.3:5010259-5010278 | MS.gene63532:CDS | 40.0% | |
| ATGCAGTGCATGTTAACCAT+AGG | + | chr3.3:5008713-5008732 | MS.gene63532:CDS | 40.0% | |
| CAACCAAGCTTCTTAATAGG+TGG | + | chr3.3:5010097-5010116 | MS.gene63532:CDS | 40.0% | |
| CAAGGGAAACTTTAAGCGAT+TGG | + | chr3.3:5010794-5010813 | MS.gene63532:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.3:5009810-5009829 | None:intergenic | 40.0% | |
| CACTTGTGGATACAATAAGG+AGG | - | chr3.3:5008786-5008805 | None:intergenic | 40.0% | |
| CTTGATGAATTCACCCATCT+TGG | - | chr3.3:5008830-5008849 | None:intergenic | 40.0% | |
| GAGCAACCAAGCTTCTTAAT+AGG | + | chr3.3:5010094-5010113 | MS.gene63532:CDS | 40.0% | |
| GCTCAAACTAATTACCTGCA+GGG | - | chr3.3:5009129-5009148 | None:intergenic | 40.0% | |
| GTACTCAGAAGCAAGTGTTT+GGG | + | chr3.3:5010758-5010777 | MS.gene63532:CDS | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.3:5010759-5010778 | MS.gene63532:CDS | 40.0% | |
| TAGACCATGTTTCCTTACCA+AGG | - | chr3.3:5009872-5009891 | None:intergenic | 40.0% | |
| TCATTCAGTCTTCCACCATA+AGG | - | chr3.3:5010513-5010532 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.3:5010435-5010454 | None:intergenic | 40.0% | |
| TTGAACACTGCAACAATAGG+TGG | - | chr3.3:5010051-5010070 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.3:5010435-5010454 | MS.gene63532:CDS | 40.0% |
| ! | ATCTTGCCGATGAATTTGCT+TGG | + | chr3.3:5009035-5009054 | MS.gene63532:CDS | 40.0% |
| ! | CGATGAATTTGCTTGGATTC+AGG | + | chr3.3:5009042-5009061 | MS.gene63532:CDS | 40.0% |
| !! | ATATGTCCAAGTGTTGGAGT+AGG | + | chr3.3:5009817-5009836 | MS.gene63532:intron | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.3:5008857-5008876 | None:intergenic | 40.0% |
| !!! | GGCTTTGAATGAGCTTTTGA+AGG | - | chr3.3:5010391-5010410 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.3:5009970-5009989 | MS.gene63532:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.3:5010426-5010445 | MS.gene63532:CDS | 45.0% | |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.3:5009112-5009131 | MS.gene63532:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.3:5008944-5008963 | MS.gene63532:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.3:5009058-5009077 | MS.gene63532:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.3:5008929-5008948 | MS.gene63532:CDS | 45.0% | |
| CTGCCACCTATTAAGAAGCT+TGG | - | chr3.3:5010103-5010122 | None:intergenic | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.3:5008919-5008938 | MS.gene63532:CDS | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.3:5009865-5009884 | MS.gene63532:CDS | 45.0% | |
| GGTACTCAGAAGCAAGTGTT+TGG | + | chr3.3:5010757-5010776 | MS.gene63532:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.3:5008926-5008945 | MS.gene63532:CDS | 45.0% | |
| TGTCTTGCAACCCTAGTTCA+GGG | - | chr3.3:5010272-5010291 | None:intergenic | 45.0% | |
| TTGTCTTGCAACCCTAGTTC+AGG | - | chr3.3:5010273-5010292 | None:intergenic | 45.0% | |
| ! | GAATTTGCTTGGATTCAGGC+TGG | + | chr3.3:5009046-5009065 | MS.gene63532:CDS | 45.0% |
| ! | TGGGGCATCAAGTACTTCAA+GGG | + | chr3.3:5010777-5010796 | MS.gene63532:CDS | 45.0% |
| ! | TTGGGGCATCAAGTACTTCA+AGG | + | chr3.3:5010776-5010795 | MS.gene63532:CDS | 45.0% |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.3:5009988-5010007 | MS.gene63532:CDS | 45.0% |
| TGACCACCTACTCCAACACT+TGG | - | chr3.3:5009826-5009845 | None:intergenic | 50.0% | |
| !! | GTGGCTTTGGTACCTTGGTA+AGG | + | chr3.3:5009857-5009876 | MS.gene63532:CDS | 50.0% |
| !! | TGTCCAAGTGTTGGAGTAGG+TGG | + | chr3.3:5009820-5009839 | MS.gene63532:intron | 50.0% |
| CACATCAGTGGAGGTGGCTT+TGG | + | chr3.3:5009844-5009863 | MS.gene63532:CDS | 55.0% | |
| CAGGGGAGGGAATTCAGAAG+AGG | - | chr3.3:5010876-5010895 | None:intergenic | 55.0% | |
| GGAGTAGGTGGTCACATCAG+TGG | + | chr3.3:5009832-5009851 | MS.gene63532:intron | 55.0% | |
| GTAGGTGGTCACATCAGTGG+AGG | + | chr3.3:5009835-5009854 | MS.gene63532:intron | 55.0% | |
| !! | TGGAGGTGGCTTTGGTACCT+TGG | + | chr3.3:5009852-5009871 | MS.gene63532:CDS | 55.0% |
| GGTGGTCACATCAGTGGAGG+TGG | + | chr3.3:5009838-5009857 | MS.gene63532:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 5008635 | 5010896 | 5008635 | ID=MS.gene63532 |
| chr3.3 | mRNA | 5008635 | 5010896 | 5008635 | ID=MS.gene63532.t1;Parent=MS.gene63532 |
| chr3.3 | exon | 5008635 | 5009133 | 5008635 | ID=MS.gene63532.t1.exon1;Parent=MS.gene63532.t1 |
| chr3.3 | CDS | 5008635 | 5009133 | 5008635 | ID=cds.MS.gene63532.t1;Parent=MS.gene63532.t1 |
| chr3.3 | exon | 5009839 | 5010896 | 5009839 | ID=MS.gene63532.t1.exon2;Parent=MS.gene63532.t1 |
| chr3.3 | CDS | 5009839 | 5010896 | 5009839 | ID=cds.MS.gene63532.t1;Parent=MS.gene63532.t1 |
| Gene Sequence |
| Protein sequence |
