Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63533.t1 | XP_003598276.1 | 87 | 399 | 44 | 1 | 1 | 391 | 78 | 476 | 4.20E-200 | 707.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63533.t1 | Q9SUC6 | 57.9 | 399 | 156 | 5 | 3 | 391 | 87 | 483 | 6.7e-138 | 491.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63533.t1 | G7J0C2 | 87.0 | 399 | 44 | 1 | 1 | 391 | 78 | 476 | 3.1e-200 | 707.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene003357 | MS.gene63533 | PPI |
| MS.gene28724 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene35083 | PPI |
| MS.gene63533 | MS.gene35113 | PPI |
| MS.gene02146 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene28742 | PPI |
| MS.gene36421 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene28723 | PPI |
| MS.gene63533 | MS.gene047977 | PPI |
| MS.gene63533 | MS.gene35102 | PPI |
| MS.gene63533 | MS.gene28726 | PPI |
| MS.gene63533 | MS.gene28717 | PPI |
| MS.gene63533 | MS.gene35106 | PPI |
| MS.gene63533 | MS.gene028353 | PPI |
| MS.gene63533 | MS.gene28727 | PPI |
| MS.gene63533 | MS.gene36444 | PPI |
| MS.gene63533 | MS.gene35114 | PPI |
| MS.gene63533 | MS.gene028354 | PPI |
| MS.gene028369 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene028347 | PPI |
| MS.gene63533 | MS.gene35103 | PPI |
| MS.gene63533 | MS.gene36437 | PPI |
| MS.gene63533 | MS.gene028357 | PPI |
| MS.gene63533 | MS.gene36439 | PPI |
| MS.gene63533 | MS.gene028346 | PPI |
| MS.gene63533 | MS.gene028352 | PPI |
| MS.gene35104 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene36435 | PPI |
| MS.gene35105 | MS.gene63533 | PPI |
| MS.gene36438 | MS.gene63533 | PPI |
| MS.gene63533 | MS.gene28725 | PPI |
| MS.gene63533 | MS.gene36445 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63533.t1 | MTR_3g009760 | 86.967 | 399 | 44 | 1 | 1 | 391 | 78 | 476 | 0.0 | 698 |
| MS.gene63533.t1 | MTR_3g009820 | 85.965 | 399 | 48 | 1 | 1 | 391 | 78 | 476 | 0.0 | 687 |
| MS.gene63533.t1 | MTR_3g009880 | 84.539 | 401 | 54 | 1 | 1 | 393 | 76 | 476 | 0.0 | 683 |
| MS.gene63533.t1 | MTR_2g031390 | 53.827 | 405 | 171 | 5 | 1 | 391 | 81 | 483 | 5.86e-162 | 466 |
| MS.gene63533.t1 | MTR_2g031420 | 58.000 | 400 | 152 | 5 | 3 | 390 | 86 | 481 | 4.21e-159 | 458 |
| MS.gene63533.t1 | MTR_4g094488 | 56.404 | 406 | 161 | 3 | 1 | 390 | 84 | 489 | 1.75e-158 | 457 |
| MS.gene63533.t1 | MTR_2g031460 | 56.858 | 401 | 157 | 5 | 3 | 391 | 86 | 482 | 1.08e-157 | 455 |
| MS.gene63533.t1 | MTR_4g094418 | 55.814 | 387 | 154 | 4 | 21 | 390 | 1 | 387 | 2.85e-154 | 449 |
| MS.gene63533.t1 | MTR_4g094492 | 56.296 | 405 | 162 | 4 | 1 | 390 | 84 | 488 | 4.88e-154 | 446 |
| MS.gene63533.t1 | MTR_4g094495 | 56.650 | 406 | 160 | 3 | 1 | 390 | 87 | 492 | 3.06e-153 | 444 |
| MS.gene63533.t1 | MTR_4g094518 | 56.188 | 404 | 161 | 5 | 1 | 390 | 82 | 483 | 4.70e-153 | 443 |
| MS.gene63533.t1 | MTR_4g094478 | 54.455 | 404 | 170 | 3 | 1 | 390 | 84 | 487 | 1.93e-152 | 442 |
| MS.gene63533.t1 | MTR_4g094520 | 52.854 | 403 | 175 | 5 | 2 | 391 | 86 | 486 | 2.57e-152 | 441 |
| MS.gene63533.t1 | MTR_2g031560 | 50.493 | 406 | 189 | 3 | 1 | 395 | 76 | 480 | 1.97e-148 | 432 |
| MS.gene63533.t1 | MTR_4g094498 | 52.882 | 399 | 178 | 3 | 1 | 390 | 90 | 487 | 3.82e-147 | 429 |
| MS.gene63533.t1 | MTR_4g094480 | 52.228 | 404 | 179 | 3 | 1 | 390 | 84 | 487 | 2.86e-145 | 423 |
| MS.gene63533.t1 | MTR_2g031590 | 50.493 | 406 | 184 | 5 | 1 | 390 | 81 | 485 | 9.50e-144 | 419 |
| MS.gene63533.t1 | MTR_2g031510 | 47.537 | 406 | 195 | 5 | 1 | 390 | 90 | 493 | 1.18e-131 | 389 |
| MS.gene63533.t1 | MTR_4g091190 | 47.750 | 400 | 194 | 5 | 1 | 388 | 82 | 478 | 1.60e-130 | 385 |
| MS.gene63533.t1 | MTR_4g091150 | 48.628 | 401 | 192 | 4 | 1 | 388 | 90 | 489 | 2.41e-130 | 385 |
| MS.gene63533.t1 | MTR_2g031490 | 46.914 | 405 | 199 | 4 | 1 | 390 | 88 | 491 | 1.51e-129 | 383 |
| MS.gene63533.t1 | MTR_4g091200 | 48.000 | 400 | 195 | 4 | 1 | 388 | 78 | 476 | 2.31e-129 | 382 |
| MS.gene63533.t1 | MTR_4g057990 | 47.103 | 397 | 200 | 3 | 1 | 388 | 77 | 472 | 4.66e-129 | 381 |
| MS.gene63533.t1 | MTR_4g088355 | 47.512 | 402 | 194 | 5 | 1 | 388 | 85 | 483 | 6.28e-129 | 382 |
| MS.gene63533.t1 | MTR_6g017205 | 46.250 | 400 | 202 | 4 | 1 | 388 | 78 | 476 | 1.05e-124 | 371 |
| MS.gene63533.t1 | MTR_4g091170 | 45.113 | 399 | 190 | 5 | 1 | 388 | 112 | 492 | 4.17e-124 | 370 |
| MS.gene63533.t1 | MTR_4g094515 | 48.387 | 403 | 193 | 5 | 1 | 390 | 703 | 1103 | 5.91e-122 | 380 |
| MS.gene63533.t1 | MTR_4g094515 | 47.160 | 405 | 193 | 6 | 1 | 388 | 91 | 491 | 6.43e-117 | 367 |
| MS.gene63533.t1 | MTR_2g023590 | 46.500 | 400 | 197 | 6 | 1 | 387 | 20 | 415 | 1.66e-121 | 360 |
| MS.gene63533.t1 | MTR_3g009850 | 86.264 | 182 | 25 | 0 | 210 | 391 | 1 | 182 | 2.81e-113 | 330 |
| MS.gene63533.t1 | MTR_1g076830 | 42.500 | 400 | 216 | 5 | 3 | 389 | 87 | 485 | 2.91e-102 | 313 |
| MS.gene63533.t1 | MTR_2g031600 | 54.321 | 162 | 66 | 1 | 1 | 154 | 82 | 243 | 5.57e-55 | 187 |
| MS.gene63533.t1 | MTR_2g031600 | 52.212 | 113 | 51 | 2 | 279 | 390 | 243 | 353 | 5.67e-33 | 128 |
| MS.gene63533.t1 | MTR_2g084370 | 73.786 | 103 | 13 | 1 | 45 | 147 | 1 | 89 | 3.74e-46 | 154 |
| MS.gene63533.t1 | MTR_2g084405 | 78.750 | 80 | 9 | 1 | 45 | 116 | 1 | 80 | 2.20e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63533.t1 | AT4G20860 | 57.750 | 400 | 157 | 5 | 3 | 392 | 87 | 484 | 1.34e-165 | 475 |
| MS.gene63533.t1 | AT5G44360 | 55.335 | 403 | 164 | 7 | 1 | 392 | 88 | 485 | 4.21e-156 | 451 |
| MS.gene63533.t1 | AT5G44360 | 55.335 | 403 | 164 | 7 | 1 | 392 | 104 | 501 | 4.63e-156 | 451 |
| MS.gene63533.t1 | AT5G44400 | 56.250 | 400 | 160 | 7 | 2 | 388 | 89 | 486 | 1.18e-154 | 447 |
| MS.gene63533.t1 | AT1G30760 | 54.229 | 402 | 168 | 5 | 3 | 390 | 108 | 507 | 1.97e-152 | 442 |
| MS.gene63533.t1 | AT1G30760 | 54.229 | 402 | 168 | 5 | 3 | 390 | 90 | 489 | 2.52e-152 | 441 |
| MS.gene63533.t1 | AT2G34790 | 52.985 | 402 | 173 | 5 | 3 | 390 | 88 | 487 | 4.18e-148 | 430 |
| MS.gene63533.t1 | AT5G44390 | 53.922 | 408 | 163 | 9 | 3 | 391 | 92 | 493 | 2.07e-144 | 421 |
| MS.gene63533.t1 | AT4G20820 | 49.497 | 398 | 184 | 6 | 1 | 389 | 88 | 477 | 7.11e-139 | 407 |
| MS.gene63533.t1 | AT5G44440 | 49.005 | 402 | 189 | 6 | 1 | 389 | 82 | 480 | 1.69e-136 | 400 |
| MS.gene63533.t1 | AT5G44440 | 49.005 | 402 | 189 | 6 | 1 | 389 | 152 | 550 | 1.50e-135 | 400 |
| MS.gene63533.t1 | AT1G26390 | 49.874 | 397 | 183 | 5 | 5 | 388 | 85 | 478 | 3.28e-131 | 387 |
| MS.gene63533.t1 | AT1G26410 | 49.242 | 396 | 185 | 5 | 5 | 388 | 109 | 500 | 3.58e-130 | 385 |
| MS.gene63533.t1 | AT1G26400 | 49.370 | 397 | 187 | 5 | 5 | 390 | 85 | 478 | 1.11e-128 | 380 |
| MS.gene63533.t1 | AT1G26420 | 49.246 | 398 | 182 | 6 | 5 | 388 | 85 | 476 | 1.41e-128 | 380 |
| MS.gene63533.t1 | AT5G44380 | 50.995 | 402 | 182 | 7 | 3 | 391 | 93 | 492 | 3.83e-128 | 380 |
| MS.gene63533.t1 | AT1G26380 | 49.620 | 395 | 181 | 5 | 7 | 388 | 87 | 476 | 7.04e-128 | 379 |
| MS.gene63533.t1 | AT5G44380 | 50.995 | 402 | 182 | 7 | 3 | 391 | 133 | 532 | 8.85e-128 | 380 |
| MS.gene63533.t1 | AT1G30700 | 48.010 | 402 | 188 | 7 | 4 | 390 | 82 | 477 | 5.88e-125 | 371 |
| MS.gene63533.t1 | AT5G44410 | 47.512 | 402 | 190 | 7 | 1 | 389 | 86 | 479 | 9.83e-125 | 371 |
| MS.gene63533.t1 | AT1G30710 | 46.366 | 399 | 193 | 7 | 7 | 390 | 91 | 483 | 3.04e-123 | 367 |
| MS.gene63533.t1 | AT1G34575 | 45.865 | 399 | 196 | 7 | 4 | 388 | 85 | 477 | 2.41e-122 | 364 |
| MS.gene63533.t1 | AT4G20800 | 47.222 | 396 | 194 | 5 | 5 | 388 | 84 | 476 | 1.39e-120 | 360 |
| MS.gene63533.t1 | AT1G11770 | 44.949 | 396 | 205 | 5 | 6 | 390 | 89 | 482 | 2.80e-119 | 357 |
| MS.gene63533.t1 | AT4G20840 | 44.417 | 403 | 207 | 6 | 1 | 390 | 90 | 488 | 8.88e-118 | 353 |
| MS.gene63533.t1 | AT1G01980 | 45.906 | 403 | 196 | 8 | 6 | 390 | 89 | 487 | 3.64e-117 | 352 |
| MS.gene63533.t1 | AT1G30730 | 45.274 | 402 | 207 | 4 | 1 | 390 | 80 | 480 | 3.07e-115 | 346 |
| MS.gene63533.t1 | AT1G30720 | 44.192 | 396 | 208 | 4 | 7 | 390 | 87 | 481 | 7.52e-114 | 342 |
| MS.gene63533.t1 | AT2G34810 | 45.771 | 402 | 199 | 5 | 6 | 390 | 92 | 491 | 2.79e-113 | 342 |
| MS.gene63533.t1 | AT4G20830 | 44.527 | 402 | 208 | 6 | 1 | 390 | 91 | 489 | 6.27e-113 | 340 |
| MS.gene63533.t1 | AT4G20830 | 44.527 | 402 | 208 | 6 | 1 | 390 | 91 | 489 | 3.27e-112 | 340 |
| MS.gene63533.t1 | AT1G30740 | 42.928 | 403 | 215 | 6 | 1 | 390 | 81 | 481 | 1.28e-107 | 327 |
| MS.gene63533.t1 | AT1G30740 | 42.928 | 403 | 215 | 6 | 1 | 390 | 84 | 484 | 1.52e-107 | 327 |
Find 74 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTGATTTAGAAGGTATT+TGG | 0.185286 | 3.3:+5042583 | MS.gene63533:CDS |
| GCTTACCTAATAGATGTAAA+TGG | 0.192403 | 3.3:+5042054 | MS.gene63533:CDS |
| TCTTGCATGGAAGATAAATT+TGG | 0.222820 | 3.3:+5042158 | MS.gene63533:CDS |
| TCCATGAACTTTACTTGCTT+TGG | 0.270151 | 3.3:-5041649 | None:intergenic |
| CGCATCAGCATTACTTATAA+TGG | 0.276583 | 3.3:+5042626 | MS.gene63533:CDS |
| CGATGAATCTGCTTGGATTC+AGG | 0.282488 | 3.3:+5041590 | MS.gene63533:CDS |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.3:+5042129 | MS.gene63533:CDS |
| CGTCCACTTTATATGCTTGT+TGG | 0.289760 | 3.3:-5042769 | None:intergenic |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.3:+5042838 | MS.gene63533:CDS |
| CTGCTTGAATTTCTGATTCA+TGG | 0.298655 | 3.3:-5041425 | None:intergenic |
| AATGAGAGTTTCCTTGAGTT+AGG | 0.320152 | 3.3:+5042399 | MS.gene63533:CDS |
| AAAGAGAGTTAAACTTGGTT+TGG | 0.320389 | 3.3:-5042341 | None:intergenic |
| CACATCAGTGGAGGAGGATT+TGG | 0.323062 | 3.3:+5041985 | MS.gene63533:CDS |
| CTGTTCAGCAAGAGTTCTAT+AGG | 0.327466 | 3.3:-5042498 | None:intergenic |
| TCCAAAGCAAGTAAAGTTCA+TGG | 0.328871 | 3.3:+5041648 | MS.gene63533:CDS |
| TTTCCTTGAGTTAGGGTTGC+AGG | 0.336327 | 3.3:+5042407 | MS.gene63533:CDS |
| TCTAAATCAGCTTCTGGTAT+AGG | 0.352720 | 3.3:-5042573 | None:intergenic |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.3:+5041606 | MS.gene63533:CDS |
| ATACCTTCTAAATCAGCTTC+TGG | 0.399342 | 3.3:-5042579 | None:intergenic |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.3:-5042816 | None:intergenic |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.3:+5042839 | MS.gene63533:CDS |
| TAATCAGACTTGGCTATGAA+TGG | 0.426890 | 3.3:-5042540 | None:intergenic |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.3:-5041538 | None:intergenic |
| CTATGTATTGCCACCTATGA+AGG | 0.438010 | 3.3:-5042248 | None:intergenic |
| CTTACCTAATAGATGTAAAT+GGG | 0.446115 | 3.3:+5042055 | MS.gene63533:CDS |
| ATACCAGAAGCTGATTTAGA+AGG | 0.450654 | 3.3:+5042576 | MS.gene63533:CDS |
| AGAATAGTTGCTCAAAATAG+TGG | 0.457619 | 3.3:+5042300 | MS.gene63533:CDS |
| GAGCAACCAAGCTCCTTCAT+AGG | 0.458493 | 3.3:+5042235 | MS.gene63533:CDS |
| ATGAGAGTTTCCTTGAGTTA+GGG | 0.464989 | 3.3:+5042400 | MS.gene63533:CDS |
| AAGACTGCATTGAAATGAGT+TGG | 0.465070 | 3.3:+5042433 | MS.gene63533:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.3:+5042852 | MS.gene63533:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.3:-5041537 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.3:-5041500 | None:intergenic |
| ATCTTGCCGATGAATCTGCT+TGG | 0.489037 | 3.3:+5041583 | MS.gene63533:CDS |
| TGCTTGAATTTCTGATTCAT+GGG | 0.498893 | 3.3:-5041424 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.3:-5042834 | None:intergenic |
| CTCTTCTACATAATCAGACT+TGG | 0.507678 | 3.3:-5042550 | None:intergenic |
| AATCAGACTTGGCTATGAAT+GGG | 0.513621 | 3.3:-5042539 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.516266 | 3.3:+5042766 | MS.gene63533:CDS |
| ATTCTTGATAGAAAAGCAAT+GGG | 0.518994 | 3.3:+5042081 | MS.gene63533:CDS |
| GAGGATTTGGTACCTTGGTA+AGG | 0.527609 | 3.3:+5041998 | MS.gene63533:CDS |
| GTTCAACATTCAAAGAACAT+TGG | 0.536377 | 3.3:+5042206 | MS.gene63533:CDS |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.3:+5041660 | MS.gene63533:CDS |
| TGTTCAGCAAGAGTTCTATA+GGG | 0.539613 | 3.3:-5042497 | None:intergenic |
| AATTCTTGATAGAAAAGCAA+TGG | 0.550485 | 3.3:+5042080 | MS.gene63533:CDS |
| TGGAGGAGGATTTGGTACCT+TGG | 0.552904 | 3.3:+5041993 | MS.gene63533:CDS |
| TTTCCTGCAACCCTAACTCA+AGG | 0.554409 | 3.3:-5042410 | None:intergenic |
| ACATACAATACTTTGTCAAG+TGG | 0.554747 | 3.3:+5042724 | MS.gene63533:CDS |
| ATGTTGAACACTGTAACAAT+AGG | 0.557897 | 3.3:-5042192 | None:intergenic |
| TTACTTATAATGGAGCCATA+TGG | 0.558832 | 3.3:+5042636 | MS.gene63533:CDS |
| TGAATTTCTGATTCATGGGA+AGG | 0.564377 | 3.3:-5041420 | None:intergenic |
| GGTGGTCACATCAGTGGAGG+AGG | 0.567357 | 3.3:+5041979 | MS.gene63533:intron |
| CTTATAATGGAGCCATATGG+TGG | 0.568422 | 3.3:+5042639 | MS.gene63533:CDS |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.3:+5041474 | MS.gene63533:CDS |
| TTGAACACTGTAACAATAGG+TGG | 0.572928 | 3.3:-5042189 | None:intergenic |
| CTGAATCCAAGCAGATTCAT+CGG | 0.574804 | 3.3:-5041589 | None:intergenic |
| AAGGAAAAGAGAGTTAAACT+TGG | 0.575300 | 3.3:-5042346 | None:intergenic |
| GAATCTGCTTGGATTCAGGC+TGG | 0.577913 | 3.3:+5041594 | MS.gene63533:CDS |
| TTGCCACCTATGAAGGAGCT+TGG | 0.579989 | 3.3:-5042241 | None:intergenic |
| GGTACCTTGGTAAGGAAACA+TGG | 0.586233 | 3.3:+5042006 | MS.gene63533:CDS |
| CATACAATACTTTGTCAAGT+GGG | 0.588214 | 3.3:+5042725 | MS.gene63533:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.3:+5041492 | MS.gene63533:CDS |
| AAACTAATTAATTACCTGCA+GGG | 0.596070 | 3.3:-5041674 | None:intergenic |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.3:+5041467 | MS.gene63533:CDS |
| GGAGTAGGTGGTCACATCAG+TGG | 0.610281 | 3.3:+5041973 | MS.gene63533:intron |
| CAAGGGAGGGAATTCAGAAG+AGG | 0.618945 | 3.3:-5043014 | None:intergenic |
| CAAGATTAATGTCGATCGAA+CGG | 0.627262 | 3.3:-5041566 | None:intergenic |
| CAACCAAGCTCCTTCATAGG+TGG | 0.630946 | 3.3:+5042238 | MS.gene63533:CDS |
| AGAACTCTTGCTGAACAGAA+CGG | 0.634545 | 3.3:+5042503 | MS.gene63533:CDS |
| TTATAATGGAGCCATATGGT+GGG | 0.651843 | 3.3:+5042640 | MS.gene63533:CDS |
| TAGACCATGTTTCCTTACCA+AGG | 0.652475 | 3.3:-5042010 | None:intergenic |
| GTAGGTGGTCACATCAGTGG+AGG | 0.667986 | 3.3:+5041976 | MS.gene63533:intron |
| ATTCAAAGAACATTGGAACA+AGG | 0.678567 | 3.3:+5042213 | MS.gene63533:CDS |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.3:+5041477 | MS.gene63533:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACTAATTAATTACCTGCA+GGG | - | chr3.3:5041677-5041696 | None:intergenic | 25.0% |
| ! | AATTCTTGATAGAAAAGCAA+TGG | + | chr3.3:5042080-5042099 | MS.gene63533:CDS | 25.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.3:5041540-5041559 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.3:5041934-5041953 | MS.gene63533:intron | 25.0% |
| ! | ATTCTTGATAGAAAAGCAAT+GGG | + | chr3.3:5042081-5042100 | MS.gene63533:CDS | 25.0% |
| ! | CTTACCTAATAGATGTAAAT+GGG | + | chr3.3:5042055-5042074 | MS.gene63533:CDS | 25.0% |
| !! | TTTTCCCATTTACATCTATT+AGG | - | chr3.3:5042062-5042081 | None:intergenic | 25.0% |
| !!! | CAATCAGTTTTATATATTGC+AGG | + | chr3.3:5042459-5042478 | MS.gene63533:CDS | 25.0% |
| AAAGAGAGTTAAACTTGGTT+TGG | - | chr3.3:5042344-5042363 | None:intergenic | 30.0% | |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.3:5042852-5042871 | MS.gene63533:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.3:5041503-5041522 | None:intergenic | 30.0% | |
| AAGGAAAAGAGAGTTAAACT+TGG | - | chr3.3:5042349-5042368 | None:intergenic | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.3:5042766-5042785 | MS.gene63533:CDS | 30.0% | |
| ACATACAATACTTTGTCAAG+TGG | + | chr3.3:5042724-5042743 | MS.gene63533:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.3:5042300-5042319 | MS.gene63533:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.3:5042839-5042858 | MS.gene63533:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.3:5041450-5041469 | MS.gene63533:CDS | 30.0% | |
| ATGTTGAACACTGTAACAAT+AGG | - | chr3.3:5042195-5042214 | None:intergenic | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.3:5042213-5042232 | MS.gene63533:CDS | 30.0% | |
| CAAACTAATTAATTACCTGC+AGG | - | chr3.3:5041678-5041697 | None:intergenic | 30.0% | |
| CATACAATACTTTGTCAAGT+GGG | + | chr3.3:5042725-5042744 | MS.gene63533:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.3:5041541-5041560 | None:intergenic | 30.0% | |
| GCTTACCTAATAGATGTAAA+TGG | + | chr3.3:5042054-5042073 | MS.gene63533:CDS | 30.0% | |
| GTTCAACATTCAAAGAACAT+TGG | + | chr3.3:5042206-5042225 | MS.gene63533:CDS | 30.0% | |
| TCTTGCATGGAAGATAAATT+TGG | + | chr3.3:5042158-5042177 | MS.gene63533:CDS | 30.0% | |
| TGCTTGAATTTCTGATTCAT+GGG | - | chr3.3:5041427-5041446 | None:intergenic | 30.0% | |
| ! | AACAAGTTTCCTTTTCTATG+TGG | - | chr3.3:5042702-5042721 | None:intergenic | 30.0% |
| ! | AAGTTTAACTCTCTTTTCCT+TGG | + | chr3.3:5042348-5042367 | MS.gene63533:CDS | 30.0% |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.3:5041451-5041470 | None:intergenic | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.3:5042985-5043004 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.3:5042984-5043003 | None:intergenic | 30.0% |
| ! | TTAAGTTTTCTTTTCCTCCA+AGG | - | chr3.3:5042368-5042387 | None:intergenic | 30.0% |
| ! | TTACTTATAATGGAGCCATA+TGG | + | chr3.3:5042636-5042655 | MS.gene63533:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.3:5042695-5042714 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.3:5042696-5042715 | None:intergenic | 30.0% |
| !! | AAGCTGATTTAGAAGGTATT+TGG | + | chr3.3:5042583-5042602 | MS.gene63533:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.3:5042098-5042117 | MS.gene63533:CDS | 30.0% |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.3:5042433-5042452 | MS.gene63533:CDS | 35.0% | |
| AATCAGACTTGGCTATGAAT+GGG | - | chr3.3:5042542-5042561 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCTTGAGTT+AGG | + | chr3.3:5042399-5042418 | MS.gene63533:CDS | 35.0% | |
| ATACCTTCTAAATCAGCTTC+TGG | - | chr3.3:5042582-5042601 | None:intergenic | 35.0% | |
| ATGAGAGTTTCCTTGAGTTA+GGG | + | chr3.3:5042400-5042419 | MS.gene63533:CDS | 35.0% | |
| CAAGATTAATGTCGATCGAA+CGG | - | chr3.3:5041569-5041588 | None:intergenic | 35.0% | |
| CGCATCAGCATTACTTATAA+TGG | + | chr3.3:5042626-5042645 | MS.gene63533:CDS | 35.0% | |
| CTCTTCTACATAATCAGACT+TGG | - | chr3.3:5042553-5042572 | None:intergenic | 35.0% | |
| CTGCTTGAATTTCTGATTCA+TGG | - | chr3.3:5041428-5041447 | None:intergenic | 35.0% | |
| CTTGTATCTATAAGCTCTCA+AGG | + | chr3.3:5041805-5041824 | MS.gene63533:intron | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.3:5042838-5042857 | MS.gene63533:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGTT+TGG | + | chr3.3:5042935-5042954 | MS.gene63533:intron | 35.0% | |
| TAATCAGACTTGGCTATGAA+TGG | - | chr3.3:5042543-5042562 | None:intergenic | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.3:5042837-5042856 | None:intergenic | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.3:5041648-5041667 | MS.gene63533:CDS | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.3:5041652-5041671 | None:intergenic | 35.0% | |
| TCTAAATCAGCTTCTGGTAT+AGG | - | chr3.3:5042576-5042595 | None:intergenic | 35.0% | |
| TGAATTTCTGATTCATGGGA+AGG | - | chr3.3:5041423-5041442 | None:intergenic | 35.0% | |
| TGTTCAGCAAGAGTTCTATA+GGG | - | chr3.3:5042500-5042519 | None:intergenic | 35.0% | |
| TTGAACACTGTAACAATAGG+TGG | - | chr3.3:5042192-5042211 | None:intergenic | 35.0% | |
| ! | ATACCAGAAGCTGATTTAGA+AGG | + | chr3.3:5042576-5042595 | MS.gene63533:CDS | 35.0% |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.3:5042690-5042709 | MS.gene63533:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.3:5042986-5043005 | None:intergenic | 35.0% |
| ! | TTATAATGGAGCCATATGGT+GGG | + | chr3.3:5042640-5042659 | MS.gene63533:CDS | 35.0% |
| ! | TTTAACTCTCTTTTCCTTGG+AGG | + | chr3.3:5042351-5042370 | MS.gene63533:CDS | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.3:5042819-5042838 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.3:5042976-5042995 | MS.gene63533:intron | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.3:5042097-5042116 | MS.gene63533:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.3:5042108-5042127 | MS.gene63533:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.3:5042145-5042164 | MS.gene63533:CDS | 35.0% |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.3:5041952-5041971 | MS.gene63533:intron | 40.0% | |
| AGAACTCTTGCTGAACAGAA+CGG | + | chr3.3:5042503-5042522 | MS.gene63533:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.3:5041951-5041970 | None:intergenic | 40.0% | |
| CGTCCACTTTATATGCTTGT+TGG | - | chr3.3:5042772-5042791 | None:intergenic | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.3:5042899-5042918 | MS.gene63533:intron | 40.0% | |
| CTATGTATTGCCACCTATGA+AGG | - | chr3.3:5042251-5042270 | None:intergenic | 40.0% | |
| CTGAATCCAAGCAGATTCAT+CGG | - | chr3.3:5041592-5041611 | None:intergenic | 40.0% | |
| CTGTTCAGCAAGAGTTCTAT+AGG | - | chr3.3:5042501-5042520 | None:intergenic | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.3:5042900-5042919 | MS.gene63533:intron | 40.0% | |
| TAGACCATGTTTCCTTACCA+AGG | - | chr3.3:5042013-5042032 | None:intergenic | 40.0% | |
| ! | CTTATAATGGAGCCATATGG+TGG | + | chr3.3:5042639-5042658 | MS.gene63533:CDS | 40.0% |
| ! | TCACTCATTTTCCCACCATA+TGG | - | chr3.3:5042654-5042673 | None:intergenic | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.3:5042918-5042937 | MS.gene63533:intron | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.3:5042917-5042936 | MS.gene63533:intron | 40.0% |
| !! | ATATGTCCAAGTGTTGGAGT+AGG | + | chr3.3:5041958-5041977 | MS.gene63533:intron | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.3:5042111-5042130 | MS.gene63533:CDS | 40.0% |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.3:5041660-5041679 | MS.gene63533:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.3:5041492-5041511 | MS.gene63533:CDS | 45.0% | |
| ATCTTGCCGATGAATCTGCT+TGG | + | chr3.3:5041583-5041602 | MS.gene63533:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.3:5041606-5041625 | MS.gene63533:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.3:5041477-5041496 | MS.gene63533:CDS | 45.0% | |
| CGATGAATCTGCTTGGATTC+AGG | + | chr3.3:5041590-5041609 | MS.gene63533:CDS | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.3:5041467-5041486 | MS.gene63533:CDS | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.3:5042898-5042917 | MS.gene63533:intron | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.3:5042006-5042025 | MS.gene63533:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.3:5041474-5041493 | MS.gene63533:CDS | 45.0% | |
| TTTCCTGCAACCCTAACTCA+AGG | - | chr3.3:5042413-5042432 | None:intergenic | 45.0% | |
| TTTCCTTGAGTTAGGGTTGC+AGG | + | chr3.3:5042407-5042426 | MS.gene63533:CDS | 45.0% | |
| ! | GAGGATTTGGTACCTTGGTA+AGG | + | chr3.3:5041998-5042017 | MS.gene63533:CDS | 45.0% |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.3:5042129-5042148 | MS.gene63533:CDS | 45.0% |
| !! | GGCTATGAATGGGCTTTTGT+AGG | - | chr3.3:5042532-5042551 | None:intergenic | 45.0% |
| CAACCAAGCTCCTTCATAGG+TGG | + | chr3.3:5042238-5042257 | MS.gene63533:CDS | 50.0% | |
| CACATCAGTGGAGGAGGATT+TGG | + | chr3.3:5041985-5042004 | MS.gene63533:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.3:5041594-5041613 | MS.gene63533:CDS | 50.0% | |
| GAGCAACCAAGCTCCTTCAT+AGG | + | chr3.3:5042235-5042254 | MS.gene63533:CDS | 50.0% | |
| TGACCACCTACTCCAACACT+TGG | - | chr3.3:5041967-5041986 | None:intergenic | 50.0% | |
| TTGCCACCTATGAAGGAGCT+TGG | - | chr3.3:5042244-5042263 | None:intergenic | 50.0% | |
| ! | TGGAGGAGGATTTGGTACCT+TGG | + | chr3.3:5041993-5042012 | MS.gene63533:CDS | 50.0% |
| !! | TGTCCAAGTGTTGGAGTAGG+TGG | + | chr3.3:5041961-5041980 | MS.gene63533:intron | 50.0% |
| GGAGTAGGTGGTCACATCAG+TGG | + | chr3.3:5041973-5041992 | MS.gene63533:intron | 55.0% | |
| GTAGGTGGTCACATCAGTGG+AGG | + | chr3.3:5041976-5041995 | MS.gene63533:intron | 55.0% | |
| GGTGGTCACATCAGTGGAGG+AGG | + | chr3.3:5041979-5041998 | MS.gene63533:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 5041414 | 5043023 | 5041414 | ID=MS.gene63533 |
| chr3.3 | mRNA | 5041414 | 5043023 | 5041414 | ID=MS.gene63533.t1;Parent=MS.gene63533 |
| chr3.3 | exon | 5041414 | 5041681 | 5041414 | ID=MS.gene63533.t1.exon1;Parent=MS.gene63533.t1 |
| chr3.3 | CDS | 5041414 | 5041681 | 5041414 | ID=cds.MS.gene63533.t1;Parent=MS.gene63533.t1 |
| chr3.3 | exon | 5041980 | 5042873 | 5041980 | ID=MS.gene63533.t1.exon2;Parent=MS.gene63533.t1 |
| chr3.3 | CDS | 5041980 | 5042873 | 5041980 | ID=cds.MS.gene63533.t1;Parent=MS.gene63533.t1 |
| chr3.3 | exon | 5042998 | 5043023 | 5042998 | ID=MS.gene63533.t1.exon3;Parent=MS.gene63533.t1 |
| chr3.3 | CDS | 5042998 | 5043023 | 5042998 | ID=cds.MS.gene63533.t1;Parent=MS.gene63533.t1 |
| Gene Sequence |
| Protein sequence |
