Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63535.t1 | AES68535.2 | 89.5 | 505 | 27 | 1 | 1 | 479 | 27 | 531 | 9.30E-258 | 899 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63535.t1 | Q9SUC6 | 53.4 | 485 | 193 | 6 | 15 | 468 | 47 | 529 | 3.0e-148 | 526.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63535.t1 | G7J0D0 | 89.5 | 505 | 27 | 1 | 1 | 479 | 27 | 531 | 6.7e-258 | 899.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63535 | MS.gene028353 | PPI |
| MS.gene28717 | MS.gene63535 | PPI |
| MS.gene028357 | MS.gene63535 | PPI |
| MS.gene63535 | MS.gene36444 | PPI |
| MS.gene28723 | MS.gene63535 | PPI |
| MS.gene35114 | MS.gene63535 | PPI |
| MS.gene63535 | MS.gene028354 | PPI |
| MS.gene63535 | MS.gene35103 | PPI |
| MS.gene28742 | MS.gene63535 | PPI |
| MS.gene63535 | MS.gene36437 | PPI |
| MS.gene63535 | MS.gene028346 | PPI |
| MS.gene28726 | MS.gene63535 | PPI |
| MS.gene028369 | MS.gene63535 | PPI |
| MS.gene35083 | MS.gene63535 | PPI |
| MS.gene63535 | MS.gene36435 | PPI |
| MS.gene028352 | MS.gene63535 | PPI |
| MS.gene35102 | MS.gene63535 | PPI |
| MS.gene36445 | MS.gene63535 | PPI |
| MS.gene28725 | MS.gene63535 | PPI |
| MS.gene36439 | MS.gene63535 | PPI |
| MS.gene35104 | MS.gene63535 | PPI |
| MS.gene35105 | MS.gene63535 | PPI |
| MS.gene36438 | MS.gene63535 | PPI |
| MS.gene28724 | MS.gene63535 | PPI |
| MS.gene028347 | MS.gene63535 | PPI |
| MS.gene35106 | MS.gene63535 | PPI |
| MS.gene02146 | MS.gene63535 | PPI |
| MS.gene28727 | MS.gene63535 | PPI |
| MS.gene63535 | MS.gene047977 | PPI |
| MS.gene003357 | MS.gene63535 | PPI |
| MS.gene35113 | MS.gene63535 | PPI |
| MS.gene36421 | MS.gene63535 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63535.t1 | MTR_3g009880 | 89.109 | 505 | 29 | 2 | 1 | 479 | 27 | 531 | 0.0 | 905 |
| MS.gene63535.t1 | MTR_3g009760 | 82.294 | 497 | 60 | 3 | 1 | 469 | 27 | 523 | 0.0 | 834 |
| MS.gene63535.t1 | MTR_3g009820 | 81.087 | 497 | 66 | 3 | 1 | 469 | 27 | 523 | 0.0 | 826 |
| MS.gene63535.t1 | MTR_4g094488 | 52.883 | 503 | 199 | 7 | 1 | 466 | 34 | 535 | 0.0 | 519 |
| MS.gene63535.t1 | MTR_2g031420 | 52.600 | 500 | 201 | 8 | 1 | 469 | 34 | 528 | 6.94e-179 | 512 |
| MS.gene63535.t1 | MTR_2g031460 | 52.016 | 496 | 202 | 8 | 1 | 465 | 34 | 524 | 2.32e-177 | 508 |
| MS.gene63535.t1 | MTR_4g094478 | 51.594 | 502 | 209 | 6 | 1 | 468 | 34 | 535 | 6.63e-177 | 508 |
| MS.gene63535.t1 | MTR_4g094492 | 51.889 | 503 | 207 | 7 | 1 | 468 | 34 | 536 | 1.86e-176 | 506 |
| MS.gene63535.t1 | MTR_2g031390 | 49.109 | 505 | 222 | 6 | 1 | 472 | 31 | 533 | 2.37e-176 | 506 |
| MS.gene63535.t1 | MTR_4g094495 | 52.191 | 502 | 204 | 7 | 1 | 466 | 37 | 538 | 1.42e-175 | 504 |
| MS.gene63535.t1 | MTR_4g094518 | 50.298 | 503 | 215 | 6 | 1 | 470 | 32 | 532 | 4.00e-169 | 487 |
| MS.gene63535.t1 | MTR_4g094418 | 54.587 | 436 | 162 | 5 | 70 | 469 | 1 | 436 | 1.02e-168 | 489 |
| MS.gene63535.t1 | MTR_2g031560 | 47.535 | 507 | 224 | 8 | 1 | 471 | 26 | 526 | 1.59e-167 | 484 |
| MS.gene63535.t1 | MTR_4g094480 | 48.200 | 500 | 225 | 6 | 1 | 466 | 34 | 533 | 8.41e-167 | 481 |
| MS.gene63535.t1 | MTR_4g094520 | 50.207 | 484 | 206 | 9 | 19 | 470 | 54 | 534 | 7.76e-165 | 476 |
| MS.gene63535.t1 | MTR_4g094498 | 47.686 | 497 | 227 | 6 | 2 | 468 | 41 | 534 | 1.66e-163 | 474 |
| MS.gene63535.t1 | MTR_2g031590 | 47.629 | 485 | 212 | 8 | 22 | 468 | 52 | 532 | 4.00e-162 | 469 |
| MS.gene63535.t1 | MTR_4g091200 | 46.107 | 488 | 230 | 7 | 11 | 466 | 38 | 524 | 2.38e-147 | 432 |
| MS.gene63535.t1 | MTR_2g031490 | 43.625 | 502 | 247 | 7 | 1 | 468 | 39 | 538 | 1.36e-146 | 430 |
| MS.gene63535.t1 | MTR_2g031510 | 43.738 | 503 | 245 | 8 | 1 | 468 | 41 | 540 | 4.73e-146 | 429 |
| MS.gene63535.t1 | MTR_3g009850 | 88.444 | 225 | 26 | 0 | 245 | 469 | 5 | 229 | 2.86e-145 | 415 |
| MS.gene63535.t1 | MTR_4g088355 | 48.026 | 456 | 205 | 6 | 42 | 466 | 77 | 531 | 1.03e-144 | 425 |
| MS.gene63535.t1 | MTR_6g017205 | 44.581 | 489 | 238 | 7 | 10 | 466 | 37 | 524 | 6.59e-143 | 421 |
| MS.gene63535.t1 | MTR_4g057990 | 45.032 | 473 | 231 | 5 | 22 | 466 | 49 | 520 | 8.35e-142 | 417 |
| MS.gene63535.t1 | MTR_4g091150 | 45.238 | 504 | 237 | 12 | 1 | 468 | 40 | 540 | 2.67e-139 | 411 |
| MS.gene63535.t1 | MTR_4g091190 | 46.491 | 456 | 210 | 7 | 42 | 466 | 74 | 526 | 2.93e-139 | 411 |
| MS.gene63535.t1 | MTR_4g094515 | 43.200 | 500 | 250 | 7 | 1 | 468 | 653 | 1150 | 3.83e-137 | 424 |
| MS.gene63535.t1 | MTR_4g094515 | 41.800 | 500 | 255 | 7 | 1 | 466 | 41 | 538 | 6.66e-130 | 404 |
| MS.gene63535.t1 | MTR_4g091170 | 45.354 | 452 | 199 | 6 | 45 | 466 | 107 | 540 | 9.50e-135 | 401 |
| MS.gene63535.t1 | MTR_2g023590 | 43.982 | 457 | 220 | 8 | 42 | 466 | 12 | 464 | 1.36e-130 | 387 |
| MS.gene63535.t1 | MTR_1g076830 | 39.451 | 474 | 252 | 8 | 26 | 465 | 60 | 532 | 1.23e-113 | 346 |
| MS.gene63535.t1 | MTR_2g031600 | 55.696 | 158 | 67 | 2 | 312 | 468 | 245 | 400 | 6.31e-56 | 191 |
| MS.gene63535.t1 | MTR_2g031600 | 43.750 | 192 | 81 | 3 | 22 | 186 | 53 | 244 | 1.87e-48 | 172 |
| MS.gene63535.t1 | MTR_2g084370 | 88.764 | 89 | 6 | 1 | 94 | 178 | 1 | 89 | 5.72e-49 | 163 |
| MS.gene63535.t1 | MTR_2g084405 | 60.000 | 85 | 6 | 3 | 94 | 150 | 1 | 85 | 4.02e-17 | 77.4 |
| MS.gene63535.t1 | MTR_8g062280 | 53.846 | 52 | 24 | 0 | 414 | 465 | 21 | 72 | 4.89e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63535.t1 | AT4G20860 | 53.846 | 455 | 180 | 6 | 42 | 468 | 77 | 529 | 3.08e-179 | 513 |
| MS.gene63535.t1 | AT5G44360 | 50.992 | 504 | 208 | 11 | 1 | 470 | 34 | 532 | 1.27e-172 | 496 |
| MS.gene63535.t1 | AT5G44360 | 50.992 | 504 | 208 | 11 | 1 | 470 | 50 | 548 | 1.67e-172 | 496 |
| MS.gene63535.t1 | AT5G44400 | 51.875 | 480 | 192 | 10 | 22 | 466 | 59 | 534 | 3.04e-166 | 480 |
| MS.gene63535.t1 | AT1G30760 | 49.378 | 482 | 205 | 8 | 18 | 466 | 55 | 530 | 4.48e-164 | 474 |
| MS.gene63535.t1 | AT1G30760 | 49.378 | 482 | 205 | 8 | 18 | 466 | 73 | 548 | 5.20e-164 | 475 |
| MS.gene63535.t1 | AT2G34790 | 49.372 | 478 | 203 | 7 | 22 | 466 | 57 | 528 | 7.21e-162 | 469 |
| MS.gene63535.t1 | AT5G44390 | 49.587 | 484 | 207 | 10 | 22 | 470 | 61 | 542 | 7.32e-157 | 456 |
| MS.gene63535.t1 | AT4G20820 | 44.600 | 500 | 240 | 8 | 1 | 471 | 38 | 529 | 5.21e-151 | 441 |
| MS.gene63535.t1 | AT5G44440 | 43.762 | 505 | 248 | 9 | 1 | 472 | 32 | 533 | 7.54e-150 | 438 |
| MS.gene63535.t1 | AT5G44440 | 43.762 | 505 | 248 | 9 | 1 | 472 | 102 | 603 | 1.02e-148 | 438 |
| MS.gene63535.t1 | AT1G26420 | 44.554 | 505 | 240 | 10 | 1 | 471 | 30 | 528 | 3.65e-148 | 434 |
| MS.gene63535.t1 | AT1G26390 | 45.021 | 482 | 230 | 8 | 22 | 471 | 52 | 530 | 6.55e-146 | 428 |
| MS.gene63535.t1 | AT5G44380 | 47.879 | 495 | 223 | 10 | 5 | 466 | 45 | 537 | 9.72e-146 | 428 |
| MS.gene63535.t1 | AT1G26380 | 46.138 | 479 | 221 | 8 | 22 | 468 | 52 | 525 | 2.02e-145 | 427 |
| MS.gene63535.t1 | AT5G44380 | 47.879 | 495 | 223 | 10 | 5 | 466 | 85 | 577 | 3.91e-145 | 428 |
| MS.gene63535.t1 | AT1G26410 | 44.421 | 484 | 234 | 8 | 18 | 470 | 72 | 551 | 1.21e-144 | 426 |
| MS.gene63535.t1 | AT5G44410 | 45.625 | 480 | 219 | 10 | 26 | 471 | 61 | 532 | 7.70e-142 | 418 |
| MS.gene63535.t1 | AT1G30710 | 42.801 | 507 | 240 | 11 | 2 | 469 | 34 | 529 | 1.41e-140 | 414 |
| MS.gene63535.t1 | AT1G34575 | 42.656 | 497 | 247 | 9 | 2 | 467 | 34 | 523 | 5.63e-140 | 413 |
| MS.gene63535.t1 | AT1G30700 | 46.555 | 479 | 214 | 10 | 26 | 468 | 54 | 526 | 2.97e-137 | 406 |
| MS.gene63535.t1 | AT1G26400 | 44.118 | 476 | 232 | 8 | 20 | 465 | 50 | 521 | 8.59e-137 | 405 |
| MS.gene63535.t1 | AT4G20840 | 40.319 | 501 | 262 | 9 | 1 | 468 | 39 | 535 | 7.44e-135 | 400 |
| MS.gene63535.t1 | AT4G20800 | 42.052 | 497 | 254 | 8 | 1 | 466 | 30 | 523 | 4.13e-134 | 398 |
| MS.gene63535.t1 | AT1G01980 | 41.667 | 504 | 256 | 9 | 1 | 468 | 33 | 534 | 8.26e-133 | 395 |
| MS.gene63535.t1 | AT1G11770 | 41.283 | 499 | 260 | 8 | 1 | 468 | 33 | 529 | 8.81e-132 | 392 |
| MS.gene63535.t1 | AT1G30730 | 43.421 | 456 | 225 | 7 | 42 | 467 | 72 | 524 | 1.01e-129 | 386 |
| MS.gene63535.t1 | AT4G20830 | 40.800 | 500 | 261 | 9 | 1 | 468 | 40 | 536 | 1.15e-128 | 384 |
| MS.gene63535.t1 | AT4G20830 | 40.800 | 500 | 261 | 9 | 1 | 468 | 40 | 536 | 4.74e-128 | 384 |
| MS.gene63535.t1 | AT1G30720 | 40.481 | 499 | 262 | 10 | 1 | 467 | 30 | 525 | 8.99e-126 | 376 |
| MS.gene63535.t1 | AT2G34810 | 39.406 | 505 | 268 | 10 | 1 | 468 | 36 | 539 | 3.65e-123 | 370 |
| MS.gene63535.t1 | AT1G30740 | 40.120 | 501 | 265 | 8 | 1 | 468 | 30 | 528 | 1.34e-117 | 355 |
| MS.gene63535.t1 | AT1G30740 | 40.120 | 501 | 265 | 8 | 1 | 468 | 33 | 531 | 1.42e-117 | 356 |
Find 88 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGCATCAAGTACTTTA+AGG | 0.182488 | 3.3:+5064379 | MS.gene63535:CDS |
| AAGCTGGTTTAGAAGGTATT+TGG | 0.185112 | 3.3:+5064045 | MS.gene63535:CDS |
| AATGAGAGTTTCCCTGAATT+AGG | 0.214946 | 3.3:+5063861 | MS.gene63535:CDS |
| CACATTAGCATTACTTATAA+TGG | 0.248787 | 3.3:+5064088 | MS.gene63535:CDS |
| TTGTCTTGCAACCCTAATTC+AGG | 0.255332 | 3.3:-5063873 | None:intergenic |
| GTTCACCATTCATAAAATTC+TGG | 0.274997 | 3.3:+5063668 | MS.gene63535:CDS |
| GGAGGTAGTGCTGCTAGTTT+TGG | 0.287867 | 3.3:+5063591 | MS.gene63535:CDS |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.3:+5064300 | MS.gene63535:CDS |
| CATCCACTTTATATGTTTGT+TGG | 0.301040 | 3.3:-5064231 | None:intergenic |
| GCTCACCTTATAGATGTAAA+TGG | 0.304217 | 3.3:+5063516 | MS.gene63535:CDS |
| TCCTTCCATTTACATCTATA+AGG | 0.320941 | 3.3:-5063521 | None:intergenic |
| CTTGATGAGTTCACCCATCT+TGG | 0.322731 | 3.3:-5062865 | None:intergenic |
| GTGGATTTGGTACCTTGTTT+AGG | 0.323328 | 3.3:+5063460 | MS.gene63535:CDS |
| TAGAGGTGGTGGAATTAATT+AGG | 0.323700 | 3.3:+5064505 | MS.gene63535:CDS |
| AATCTCTTTGCTGCATAGTA+TGG | 0.329557 | 3.3:-5062938 | None:intergenic |
| CACATCAGTGGAGGTGGATT+TGG | 0.356454 | 3.3:+5063447 | MS.gene63535:CDS |
| ATGAGAGTTTCCCTGAATTA+GGG | 0.357011 | 3.3:+5063862 | MS.gene63535:CDS |
| CTGTTAAGCAAGAGTTCTAT+AGG | 0.363323 | 3.3:-5063960 | None:intergenic |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.3:-5064035 | None:intergenic |
| GCTACTCAGAAGCAAGTGTT+TGG | 0.368108 | 3.3:+5064360 | MS.gene63535:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.3:+5063096 | MS.gene63535:CDS |
| AGCAGCACTACCTCCTCTTA+TGG | 0.383413 | 3.3:-5063583 | None:intergenic |
| AAATGAATCTGCTTGGATTC+AGG | 0.391403 | 3.3:+5063080 | MS.gene63535:CDS |
| TAGTCAGATTTGGCTTTGAA+TGG | 0.394226 | 3.3:-5064002 | None:intergenic |
| TTCAGAATAGCTACTAACTA+TGG | 0.395170 | 3.3:-5062773 | None:intergenic |
| TCACTCATTCTTCCACCATA+TGG | 0.398978 | 3.3:-5064113 | None:intergenic |
| AGAATAGTTGCTCAAACTAA+TGG | 0.401600 | 3.3:+5063762 | MS.gene63535:CDS |
| TGGGGCATCAAGTACTTTAA+GGG | 0.402731 | 3.3:+5064380 | MS.gene63535:CDS |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.3:-5064041 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.3:+5064038 | MS.gene63535:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.3:-5064278 | None:intergenic |
| CTACTCAGAAGCAAGTGTTT+GGG | 0.418460 | 3.3:+5064361 | MS.gene63535:CDS |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.3:+5064301 | MS.gene63535:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.3:-5063028 | None:intergenic |
| ATGTTTAATTCTCTCTTCCT+TGG | 0.438442 | 3.3:+5063810 | MS.gene63535:CDS |
| CCACAAAACTCATTCATAGA+TGG | 0.453753 | 3.3:+5063700 | MS.gene63535:CDS |
| TCATTCTTGCATGGAAGATA+AGG | 0.459271 | 3.3:+5063616 | MS.gene63535:CDS |
| ATCTTGCAAATGAATCTGCT+TGG | 0.463262 | 3.3:+5063073 | MS.gene63535:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.467290 | 3.3:+5064314 | MS.gene63535:CDS |
| GATTCTTAATAGAAAATCAA+TGG | 0.470027 | 3.3:+5063542 | MS.gene63535:CDS |
| TAAGGGAAACTTTAAGCGAT+TGG | 0.470910 | 3.3:+5064397 | MS.gene63535:CDS |
| TGTCTTGCAACCCTAATTCA+GGG | 0.475992 | 3.3:-5063872 | None:intergenic |
| AAGACTGCATTGAAATGAGT+TGG | 0.478707 | 3.3:+5063895 | MS.gene63535:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.3:-5063027 | None:intergenic |
| AAGATAGTCCTTCATAATCA+TGG | 0.479154 | 3.3:-5062990 | None:intergenic |
| AGTCAGATTTGGCTTTGAAT+GGG | 0.479319 | 3.3:-5064001 | None:intergenic |
| CATTGAAACAAAATCCAAGA+TGG | 0.486669 | 3.3:+5062851 | MS.gene63535:CDS |
| AGTCCAACAAACATATAAAG+TGG | 0.489218 | 3.3:+5064228 | MS.gene63535:CDS |
| ATTCATAAAATTCTGGAAGA+AGG | 0.493097 | 3.3:+5063675 | MS.gene63535:CDS |
| TTCAATGATTCCAACACTTG+TGG | 0.495973 | 3.3:-5062835 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.3:-5064296 | None:intergenic |
| ATACTATGCAGCAAAGAGAT+TGG | 0.503786 | 3.3:+5062940 | MS.gene63535:CDS |
| CTTGTACAACATACAATACA+TGG | 0.505452 | 3.3:+5064178 | MS.gene63535:CDS |
| TTCCAACACTTGTGGATACA+AGG | 0.515360 | 3.3:-5062827 | None:intergenic |
| GGCGGTCACATCAGTGGAGG+TGG | 0.518000 | 3.3:+5063441 | MS.gene63535:intron |
| ATTCTTAATAGAAAATCAAT+GGG | 0.522100 | 3.3:+5063543 | MS.gene63535:CDS |
| ATCCTTGTATCCACAAGTGT+TGG | 0.532948 | 3.3:+5062825 | MS.gene63535:CDS |
| GGTACCTTGTTTAGGAAACA+TGG | 0.537180 | 3.3:+5063468 | MS.gene63535:CDS |
| TTACTTATAATGGAACCATA+TGG | 0.538421 | 3.3:+5064098 | MS.gene63535:CDS |
| TCTTGCATGGAAGATAAGGT+TGG | 0.542256 | 3.3:+5063620 | MS.gene63535:CDS |
| GTCATTACTCCTTCACATAG+AGG | 0.545004 | 3.3:+5064488 | MS.gene63535:CDS |
| TGAATTTCTGATTCATGAGA+AGG | 0.547415 | 3.3:-5062910 | None:intergenic |
| ATGGTGAACACTGTAACAAT+AGG | 0.551113 | 3.3:-5063654 | None:intergenic |
| ATTGAAACAAAATCCAAGAT+GGG | 0.551532 | 3.3:+5062852 | MS.gene63535:CDS |
| CAAGATTAATGTCAATCGAA+CGG | 0.553223 | 3.3:-5063056 | None:intergenic |
| ACTCCTTCACATAGAGGTGG+TGG | 0.555530 | 3.3:+5064494 | MS.gene63535:CDS |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.3:+5064029 | MS.gene63535:CDS |
| ACATACAATACATGGTCATG+TGG | 0.564962 | 3.3:+5064186 | MS.gene63535:CDS |
| GATTGGTGTGCAAATTAGAG+TGG | 0.569189 | 3.3:+5062957 | MS.gene63535:CDS |
| GTGCAAATTAGAGTGGTTAG+TGG | 0.589648 | 3.3:+5062964 | MS.gene63535:CDS |
| ATAGGGATCTTGATATAGGA+AGG | 0.589814 | 3.3:+5064318 | MS.gene63535:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.3:+5062982 | MS.gene63535:CDS |
| GAATCTGCTTGGATTCAGGC+TGG | 0.594184 | 3.3:+5063084 | MS.gene63535:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.597018 | 3.3:+5064362 | MS.gene63535:CDS |
| GTGAACACTGTAACAATAGG+TGG | 0.602884 | 3.3:-5063651 | None:intergenic |
| GGAATAGGCGGTCACATCAG+TGG | 0.603755 | 3.3:+5063435 | MS.gene63535:intron |
| GGAGTAATGACATTTAGTAG+AGG | 0.607260 | 3.3:-5064476 | None:intergenic |
| GAAGGTGTCATAATGAGAAG+AGG | 0.607937 | 3.3:-5062892 | None:intergenic |
| ACCTTATAGATGTAAATGGA+AGG | 0.611838 | 3.3:+5063520 | MS.gene63535:CDS |
| CTTATAATGGAACCATATGG+TGG | 0.625352 | 3.3:+5064101 | MS.gene63535:CDS |
| ATTCCACCACCTCTATGTGA+AGG | 0.628617 | 3.3:-5064497 | None:intergenic |
| CATACAATACATGGTCATGT+GGG | 0.628675 | 3.3:+5064187 | MS.gene63535:CDS |
| TAGACCATGTTTCCTAAACA+AGG | 0.647398 | 3.3:-5063472 | None:intergenic |
| TAAGGTGAGCATCAACAACA+TGG | 0.661883 | 3.3:-5063503 | None:intergenic |
| ATAGGCGGTCACATCAGTGG+AGG | 0.689151 | 3.3:+5063438 | MS.gene63535:intron |
| ATTACTCCTTCACATAGAGG+TGG | 0.692038 | 3.3:+5064491 | MS.gene63535:CDS |
| AAGGAAGAGAGAATTAAACA+TGG | 0.696240 | 3.3:-5063808 | None:intergenic |
| CAAATTAGAGTGGTTAGTGG+TGG | 0.705025 | 3.3:+5062967 | MS.gene63535:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTTAATAGAAAATCAAT+GGG | + | chr3.3:5063543-5063562 | MS.gene63535:CDS | 15.0% |
| !! | GATTCTTAATAGAAAATCAA+TGG | + | chr3.3:5063542-5063561 | MS.gene63535:CDS | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.3:5063030-5063049 | None:intergenic | 25.0% |
| ! | ATTGAAACAAAATCCAAGAT+GGG | + | chr3.3:5062852-5062871 | MS.gene63535:CDS | 25.0% |
| ! | CACATTAGCATTACTTATAA+TGG | + | chr3.3:5064088-5064107 | MS.gene63535:CDS | 25.0% |
| !! | ATTCATAAAATTCTGGAAGA+AGG | + | chr3.3:5063675-5063694 | MS.gene63535:CDS | 25.0% |
| !! | TTAAGTTTTCTTTTCTTCCA+AGG | - | chr3.3:5063830-5063849 | None:intergenic | 25.0% |
| !! | TTACTTATAATGGAACCATA+TGG | + | chr3.3:5064098-5064117 | MS.gene63535:CDS | 25.0% |
| !! | TTCTTCCAGAATTTTATGAA+TGG | - | chr3.3:5063676-5063695 | None:intergenic | 25.0% |
| !!! | ATTAAGTTGTTTTTCCAAAC+AGG | + | chr3.3:5063396-5063415 | MS.gene63535:intron | 25.0% |
| !!! | TTAAGTTGTTTTTCCAAACA+GGG | + | chr3.3:5063397-5063416 | MS.gene63535:intron | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.3:5064314-5064333 | MS.gene63535:CDS | 30.0% | |
| AAGATAGTCCTTCATAATCA+TGG | - | chr3.3:5062993-5063012 | None:intergenic | 30.0% | |
| AAGGAAGAGAGAATTAAACA+TGG | - | chr3.3:5063811-5063830 | None:intergenic | 30.0% | |
| ACCTTATAGATGTAAATGGA+AGG | + | chr3.3:5063520-5063539 | MS.gene63535:CDS | 30.0% | |
| AGAATAGTTGCTCAAACTAA+TGG | + | chr3.3:5063762-5063781 | MS.gene63535:CDS | 30.0% | |
| AGCACAAGCAAATTATAAGA+CGG | + | chr3.3:5063205-5063224 | MS.gene63535:intron | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.3:5064301-5064320 | MS.gene63535:CDS | 30.0% | |
| AGTCCAACAAACATATAAAG+TGG | + | chr3.3:5064228-5064247 | MS.gene63535:CDS | 30.0% | |
| ATGTTTAATTCTCTCTTCCT+TGG | + | chr3.3:5063810-5063829 | MS.gene63535:CDS | 30.0% | |
| CAAGATTAATGTCAATCGAA+CGG | - | chr3.3:5063059-5063078 | None:intergenic | 30.0% | |
| CATCCACTTTATATGTTTGT+TGG | - | chr3.3:5064234-5064253 | None:intergenic | 30.0% | |
| CATTGAAACAAAATCCAAGA+TGG | + | chr3.3:5062851-5062870 | MS.gene63535:CDS | 30.0% | |
| CTTGTACAACATACAATACA+TGG | + | chr3.3:5064178-5064197 | MS.gene63535:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.3:5063031-5063050 | None:intergenic | 30.0% | |
| GTTCACCATTCATAAAATTC+TGG | + | chr3.3:5063668-5063687 | MS.gene63535:CDS | 30.0% | |
| TCCTTCCATTTACATCTATA+AGG | - | chr3.3:5063524-5063543 | None:intergenic | 30.0% | |
| TGAATTTCTGATTCATGAGA+AGG | - | chr3.3:5062913-5062932 | None:intergenic | 30.0% | |
| TTCAGAATAGCTACTAACTA+TGG | - | chr3.3:5062776-5062795 | None:intergenic | 30.0% | |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.3:5064447-5064466 | None:intergenic | 30.0% |
| ! | CTCTTTTACATAGTCAGATT+TGG | - | chr3.3:5064015-5064034 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.3:5064446-5064465 | None:intergenic | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+AGG | + | chr3.3:5063921-5063940 | MS.gene63535:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.3:5063560-5063579 | MS.gene63535:CDS | 30.0% |
| !!! | GATAGACACTGTTTTTTCAT+TGG | - | chr3.3:5062800-5062819 | None:intergenic | 30.0% |
| AAATGAATCTGCTTGGATTC+AGG | + | chr3.3:5063080-5063099 | MS.gene63535:CDS | 35.0% | |
| AAGACTGCATTGAAATGAGT+TGG | + | chr3.3:5063895-5063914 | MS.gene63535:CDS | 35.0% | |
| AAGTCAAACTAATTCACCTG+AGG | - | chr3.3:5063169-5063188 | None:intergenic | 35.0% | |
| AATCTCTTTGCTGCATAGTA+TGG | - | chr3.3:5062941-5062960 | None:intergenic | 35.0% | |
| AATGAGAGTTTCCCTGAATT+AGG | + | chr3.3:5063861-5063880 | MS.gene63535:CDS | 35.0% | |
| ACATACAATACATGGTCATG+TGG | + | chr3.3:5064186-5064205 | MS.gene63535:CDS | 35.0% | |
| AGTCAAACTAATTCACCTGA+GGG | - | chr3.3:5063168-5063187 | None:intergenic | 35.0% | |
| ATACTATGCAGCAAAGAGAT+TGG | + | chr3.3:5062940-5062959 | MS.gene63535:CDS | 35.0% | |
| ATAGGGATCTTGATATAGGA+AGG | + | chr3.3:5064318-5064337 | MS.gene63535:CDS | 35.0% | |
| ATCTTGCAAATGAATCTGCT+TGG | + | chr3.3:5063073-5063092 | MS.gene63535:CDS | 35.0% | |
| ATGAGAGTTTCCCTGAATTA+GGG | + | chr3.3:5063862-5063881 | MS.gene63535:CDS | 35.0% | |
| ATGGTGAACACTGTAACAAT+AGG | - | chr3.3:5063657-5063676 | None:intergenic | 35.0% | |
| CATACAATACATGGTCATGT+GGG | + | chr3.3:5064187-5064206 | MS.gene63535:CDS | 35.0% | |
| CCACAAAACTCATTCATAGA+TGG | + | chr3.3:5063700-5063719 | MS.gene63535:CDS | 35.0% | |
| CTGTTAAGCAAGAGTTCTAT+AGG | - | chr3.3:5063963-5063982 | None:intergenic | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.3:5064300-5064319 | MS.gene63535:CDS | 35.0% | |
| GCTCACCTTATAGATGTAAA+TGG | + | chr3.3:5063516-5063535 | MS.gene63535:CDS | 35.0% | |
| GGAGTAATGACATTTAGTAG+AGG | - | chr3.3:5064479-5064498 | None:intergenic | 35.0% | |
| TAAGGGAAACTTTAAGCGAT+TGG | + | chr3.3:5064397-5064416 | MS.gene63535:CDS | 35.0% | |
| TAGACCATGTTTCCTAAACA+AGG | - | chr3.3:5063475-5063494 | None:intergenic | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.3:5064299-5064318 | None:intergenic | 35.0% | |
| TCATTCTTGCATGGAAGATA+AGG | + | chr3.3:5063616-5063635 | MS.gene63535:CDS | 35.0% | |
| TCCAAAGCAAGTAAAGTTCA+TGG | + | chr3.3:5063138-5063157 | MS.gene63535:intron | 35.0% | |
| TCCATGAACTTTACTTGCTT+TGG | - | chr3.3:5063142-5063161 | None:intergenic | 35.0% | |
| TTCAATGATTCCAACACTTG+TGG | - | chr3.3:5062838-5062857 | None:intergenic | 35.0% | |
| ! | CCATCTATGAATGAGTTTTG+TGG | - | chr3.3:5063703-5063722 | None:intergenic | 35.0% |
| ! | CTTATAATGGAACCATATGG+TGG | + | chr3.3:5064101-5064120 | MS.gene63535:CDS | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.3:5064448-5064467 | None:intergenic | 35.0% |
| ! | TACAAGTTTCCTTTTCTGTG+TGG | - | chr3.3:5064164-5064183 | None:intergenic | 35.0% |
| ! | TTCCTTTTCTGTGTGGAAAA+GGG | - | chr3.3:5064157-5064176 | None:intergenic | 35.0% |
| ! | TTTCCTTTTCTGTGTGGAAA+AGG | - | chr3.3:5064158-5064177 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.3:5064045-5064064 | MS.gene63535:CDS | 35.0% |
| !! | AGTCAGATTTGGCTTTGAAT+GGG | - | chr3.3:5064004-5064023 | None:intergenic | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.3:5064281-5064300 | None:intergenic | 35.0% |
| !! | TAGTCAGATTTGGCTTTGAA+TGG | - | chr3.3:5064005-5064024 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.3:5064438-5064457 | MS.gene63535:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.3:5063559-5063578 | MS.gene63535:CDS | 35.0% |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.3:5064044-5064063 | None:intergenic | 40.0% | |
| ATCCTTGTATCCACAAGTGT+TGG | + | chr3.3:5062825-5062844 | MS.gene63535:CDS | 40.0% | |
| ATTACTCCTTCACATAGAGG+TGG | + | chr3.3:5064491-5064510 | MS.gene63535:CDS | 40.0% | |
| CAAATTAGAGTGGTTAGTGG+TGG | + | chr3.3:5062967-5062986 | MS.gene63535:CDS | 40.0% | |
| CTACTCAGAAGCAAGTGTTT+GGG | + | chr3.3:5064361-5064380 | MS.gene63535:CDS | 40.0% | |
| GATTGGTGTGCAAATTAGAG+TGG | + | chr3.3:5062957-5062976 | MS.gene63535:CDS | 40.0% | |
| GTCAAACTAATTCACCTGAG+GGG | - | chr3.3:5063167-5063186 | None:intergenic | 40.0% | |
| GTCATTACTCCTTCACATAG+AGG | + | chr3.3:5064488-5064507 | MS.gene63535:CDS | 40.0% | |
| GTGAACACTGTAACAATAGG+TGG | - | chr3.3:5063654-5063673 | None:intergenic | 40.0% | |
| GTGCAAATTAGAGTGGTTAG+TGG | + | chr3.3:5062964-5062983 | MS.gene63535:CDS | 40.0% | |
| TAAGGTGAGCATCAACAACA+TGG | - | chr3.3:5063506-5063525 | None:intergenic | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.3:5064362-5064381 | MS.gene63535:CDS | 40.0% | |
| TCACTCATTCTTCCACCATA+TGG | - | chr3.3:5064116-5064135 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.3:5064038-5064057 | None:intergenic | 40.0% | |
| TCTTGCATGGAAGATAAGGT+TGG | + | chr3.3:5063620-5063639 | MS.gene63535:CDS | 40.0% | |
| TGTCTTGCAACCCTAATTCA+GGG | - | chr3.3:5063875-5063894 | None:intergenic | 40.0% | |
| TTCCAACACTTGTGGATACA+AGG | - | chr3.3:5062830-5062849 | None:intergenic | 40.0% | |
| TTGTCTTGCAACCCTAATTC+AGG | - | chr3.3:5063876-5063895 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.3:5064038-5064057 | MS.gene63535:CDS | 40.0% |
| ! | ATCCCTTTTCCACACAGAAA+AGG | + | chr3.3:5064152-5064171 | MS.gene63535:CDS | 40.0% |
| ! | GGTACCTTGTTTAGGAAACA+TGG | + | chr3.3:5063468-5063487 | MS.gene63535:CDS | 40.0% |
| ! | GTGGATTTGGTACCTTGTTT+AGG | + | chr3.3:5063460-5063479 | MS.gene63535:CDS | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.3:5064380-5064399 | MS.gene63535:CDS | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.3:5064379-5064398 | MS.gene63535:CDS | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.3:5062895-5062914 | None:intergenic | 40.0% |
| !!! | GATGTTTTTTGGGCCATAAG+AGG | + | chr3.3:5063570-5063589 | MS.gene63535:CDS | 40.0% |
| !!! | GTTTTGGAGTCATTCTTGCA+TGG | + | chr3.3:5063607-5063626 | MS.gene63535:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.3:5064029-5064048 | MS.gene63535:CDS | 45.0% | |
| AAAGTTCATGGATTCCCCTC+AGG | + | chr3.3:5063150-5063169 | MS.gene63535:intron | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.3:5062982-5063001 | MS.gene63535:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.3:5063096-5063115 | MS.gene63535:CDS | 45.0% | |
| ATTCCACCACCTCTATGTGA+AGG | - | chr3.3:5064500-5064519 | None:intergenic | 45.0% | |
| CTTGATGAGTTCACCCATCT+TGG | - | chr3.3:5062868-5062887 | None:intergenic | 45.0% | |
| GCTACTCAGAAGCAAGTGTT+TGG | + | chr3.3:5064360-5064379 | MS.gene63535:CDS | 45.0% | |
| !!! | GGCTTTGAATGGGCTTTTGT+AGG | - | chr3.3:5063994-5064013 | None:intergenic | 45.0% |
| !!! | GTTTTTTGGGCCATAAGAGG+AGG | + | chr3.3:5063573-5063592 | MS.gene63535:CDS | 45.0% |
| ACTCCTTCACATAGAGGTGG+TGG | + | chr3.3:5064494-5064513 | MS.gene63535:CDS | 50.0% | |
| AGCAGCACTACCTCCTCTTA+TGG | - | chr3.3:5063586-5063605 | None:intergenic | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.3:5063084-5063103 | MS.gene63535:CDS | 50.0% | |
| ! | CACATCAGTGGAGGTGGATT+TGG | + | chr3.3:5063447-5063466 | MS.gene63535:CDS | 50.0% |
| !! | GGAGGTAGTGCTGCTAGTTT+TGG | + | chr3.3:5063591-5063610 | MS.gene63535:CDS | 50.0% |
| !! | TTGTGTCCGAGCGTTGGAAT+AGG | + | chr3.3:5063420-5063439 | MS.gene63535:intron | 50.0% |
| ATAGGCGGTCACATCAGTGG+AGG | + | chr3.3:5063438-5063457 | MS.gene63535:intron | 55.0% | |
| GGAATAGGCGGTCACATCAG+TGG | + | chr3.3:5063435-5063454 | MS.gene63535:intron | 55.0% | |
| TGACCGCCTATTCCAACGCT+CGG | - | chr3.3:5063429-5063448 | None:intergenic | 55.0% | |
| !! | TGTCCGAGCGTTGGAATAGG+CGG | + | chr3.3:5063423-5063442 | MS.gene63535:intron | 55.0% |
| ACAGGGTTGTGTCCGAGCGT+TGG | + | chr3.3:5063414-5063433 | MS.gene63535:intron | 60.0% | |
| CGCTCGGACACAACCCTGTT+TGG | - | chr3.3:5063413-5063432 | None:intergenic | 60.0% | |
| GGCGGTCACATCAGTGGAGG+TGG | + | chr3.3:5063441-5063460 | MS.gene63535:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 5062757 | 5064526 | 5062757 | ID=MS.gene63535 |
| chr3.3 | mRNA | 5062757 | 5064526 | 5062757 | ID=MS.gene63535.t1;Parent=MS.gene63535 |
| chr3.3 | exon | 5062757 | 5063117 | 5062757 | ID=MS.gene63535.t1.exon1;Parent=MS.gene63535.t1 |
| chr3.3 | CDS | 5062757 | 5063117 | 5062757 | ID=cds.MS.gene63535.t1;Parent=MS.gene63535.t1 |
| chr3.3 | exon | 5063442 | 5064526 | 5063442 | ID=MS.gene63535.t1.exon2;Parent=MS.gene63535.t1 |
| chr3.3 | CDS | 5063442 | 5064526 | 5063442 | ID=cds.MS.gene63535.t1;Parent=MS.gene63535.t1 |
| Gene Sequence |
| Protein sequence |
