Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65294.t1 | XP_003606498.1 | 95.6 | 114 | 5 | 0 | 15 | 128 | 58 | 171 | 5.40E-56 | 226.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65294.t1 | Q9M1E7 | 74.6 | 114 | 29 | 0 | 15 | 128 | 58 | 171 | 2.0e-46 | 186.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65294.t1 | G7JRH3 | 95.6 | 114 | 5 | 0 | 15 | 128 | 58 | 171 | 3.9e-56 | 226.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene65294 | 0.81099 | 8.76E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene65294 | 0.827724 | 1.34E-54 | -1.69E-46 |
| MS.gene049254 | MS.gene65294 | 0.801998 | 6.91E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene65294 | 0.871568 | 6.19E-67 | -1.69E-46 |
| MS.gene049499 | MS.gene65294 | 0.848982 | 4.33E-60 | -1.69E-46 |
| MS.gene049983 | MS.gene65294 | 0.823734 | 1.18E-53 | -1.69E-46 |
| MS.gene050414 | MS.gene65294 | 0.806257 | 8.98E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene65294 | 0.812141 | 4.92E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene65294 | 0.809579 | 1.76E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene65294 | 0.828605 | 8.19E-55 | -1.69E-46 |
| MS.gene051676 | MS.gene65294 | 0.801932 | 7.13E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene65294 | 0.839074 | 1.97E-57 | -1.69E-46 |
| MS.gene051832 | MS.gene65294 | 0.805033 | 1.62E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene65294 | 0.802526 | 5.38E-49 | -1.69E-46 |
| MS.gene052242 | MS.gene65294 | -0.814093 | 1.84E-51 | -1.69E-46 |
| MS.gene052686 | MS.gene65294 | 0.809275 | 2.05E-50 | -1.69E-46 |
| MS.gene052818 | MS.gene65294 | -0.803624 | 3.19E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene65294 | 0.808012 | 3.82E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene65294 | 0.842908 | 1.94E-58 | -1.69E-46 |
| MS.gene053340 | MS.gene65294 | 0.807845 | 4.14E-50 | -1.69E-46 |
| MS.gene053726 | MS.gene65294 | 0.814058 | 1.87E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene65294 | 0.871046 | 9.21E-67 | -1.69E-46 |
| MS.gene054222 | MS.gene65294 | 0.857638 | 1.42E-62 | -1.69E-46 |
| MS.gene054479 | MS.gene65294 | 0.829379 | 5.32E-55 | -1.69E-46 |
| MS.gene054576 | MS.gene65294 | 0.80586 | 1.09E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene65294 | 0.827961 | 1.17E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene65294 | 0.833995 | 3.87E-56 | -1.69E-46 |
| MS.gene054957 | MS.gene65294 | 0.823824 | 1.13E-53 | -1.69E-46 |
| MS.gene05539 | MS.gene65294 | 0.802385 | 5.75E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene65294 | 0.814449 | 1.53E-51 | -1.69E-46 |
| MS.gene056403 | MS.gene65294 | 0.822138 | 2.78E-53 | -1.69E-46 |
| MS.gene056605 | MS.gene65294 | 0.812697 | 3.72E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene65294 | 0.806148 | 9.47E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene65294 | 0.800914 | 1.15E-48 | -1.69E-46 |
| MS.gene056786 | MS.gene65294 | 0.807212 | 5.64E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene65294 | 0.809666 | 1.69E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene65294 | 0.835351 | 1.77E-56 | -1.69E-46 |
| MS.gene057155 | MS.gene65294 | 0.83801 | 3.71E-57 | -1.69E-46 |
| MS.gene05781 | MS.gene65294 | 0.815743 | 7.91E-52 | -1.69E-46 |
| MS.gene057893 | MS.gene65294 | -0.807448 | 5.03E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene65294 | 0.814237 | 1.71E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene65294 | 0.835432 | 1.68E-56 | -1.69E-46 |
| MS.gene058223 | MS.gene65294 | -0.800555 | 1.36E-48 | -1.69E-46 |
| MS.gene058279 | MS.gene65294 | 0.831816 | 1.35E-55 | -1.69E-46 |
| MS.gene058592 | MS.gene65294 | 0.813177 | 2.92E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene65294 | 0.802046 | 6.76E-49 | -1.69E-46 |
| MS.gene05878 | MS.gene65294 | 0.814728 | 1.33E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene65294 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene65294 | 0.803126 | 4.04E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene65294 | 0.856791 | 2.53E-62 | -1.69E-46 |
| MS.gene05985 | MS.gene65294 | -0.838794 | 2.33E-57 | -1.69E-46 |
| MS.gene059901 | MS.gene65294 | -0.821397 | 4.13E-53 | -1.69E-46 |
| MS.gene060012 | MS.gene65294 | 0.842966 | 1.87E-58 | -1.69E-46 |
| MS.gene06018 | MS.gene65294 | 0.834944 | 2.24E-56 | -1.69E-46 |
| MS.gene060527 | MS.gene65294 | 0.811645 | 6.31E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene65294 | 0.800616 | 1.33E-48 | -1.69E-46 |
| MS.gene060777 | MS.gene65294 | 0.804016 | 2.64E-49 | -1.69E-46 |
| MS.gene060840 | MS.gene65294 | 0.812211 | 4.75E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene65294 | 0.820454 | 6.80E-53 | -1.69E-46 |
| MS.gene060933 | MS.gene65294 | 0.838623 | 2.58E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene65294 | 0.829308 | 5.54E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene65294 | 0.851231 | 1.02E-60 | -1.69E-46 |
| MS.gene061100 | MS.gene65294 | 0.810966 | 8.86E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene65294 | 0.812836 | 3.47E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65294.t1 | MTR_4g061010 | 95.614 | 114 | 5 | 0 | 15 | 128 | 58 | 171 | 4.76e-77 | 229 |
| MS.gene65294.t1 | MTR_6g033335 | 46.429 | 112 | 54 | 1 | 17 | 128 | 59 | 164 | 1.13e-28 | 105 |
| MS.gene65294.t1 | MTR_4g129670 | 46.491 | 114 | 59 | 1 | 15 | 128 | 57 | 168 | 3.30e-28 | 104 |
| MS.gene65294.t1 | MTR_8g066790 | 40.179 | 112 | 63 | 1 | 17 | 128 | 59 | 166 | 4.09e-20 | 83.2 |
| MS.gene65294.t1 | MTR_1g095530 | 42.105 | 114 | 60 | 1 | 15 | 128 | 57 | 164 | 5.02e-20 | 83.2 |
| MS.gene65294.t1 | MTR_8g097280 | 30.702 | 114 | 75 | 1 | 15 | 128 | 58 | 167 | 8.04e-17 | 74.7 |
| MS.gene65294.t1 | MTR_4g112570 | 36.957 | 92 | 58 | 0 | 17 | 108 | 62 | 153 | 3.82e-16 | 72.4 |
| MS.gene65294.t1 | MTR_5g030070 | 40.367 | 109 | 61 | 1 | 20 | 128 | 62 | 166 | 5.39e-15 | 69.3 |
| MS.gene65294.t1 | MTR_8g101600 | 38.596 | 114 | 66 | 1 | 15 | 128 | 57 | 166 | 7.03e-15 | 68.9 |
| MS.gene65294.t1 | MTR_2g056130 | 33.333 | 90 | 60 | 0 | 15 | 104 | 57 | 146 | 3.34e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65294.t1 | AT3G45600 | 74.561 | 114 | 29 | 0 | 15 | 128 | 58 | 171 | 7.12e-63 | 191 |
| MS.gene65294.t1 | AT3G45600 | 74.561 | 114 | 29 | 0 | 15 | 128 | 58 | 171 | 1.53e-61 | 190 |
| MS.gene65294.t1 | AT5G60220 | 63.158 | 114 | 42 | 0 | 15 | 128 | 58 | 171 | 5.88e-48 | 156 |
| MS.gene65294.t1 | AT2G23810 | 43.860 | 114 | 62 | 1 | 15 | 128 | 58 | 169 | 1.66e-27 | 102 |
| MS.gene65294.t1 | AT3G12090 | 42.982 | 114 | 59 | 2 | 15 | 128 | 57 | 164 | 3.24e-23 | 91.7 |
| MS.gene65294.t1 | AT4G30430 | 39.474 | 114 | 67 | 1 | 15 | 128 | 58 | 169 | 3.40e-22 | 88.6 |
| MS.gene65294.t1 | AT4G28050 | 37.719 | 114 | 69 | 1 | 15 | 128 | 58 | 169 | 2.41e-21 | 86.3 |
| MS.gene65294.t1 | AT5G46700 | 38.938 | 113 | 65 | 1 | 16 | 128 | 58 | 166 | 1.57e-20 | 84.3 |
| MS.gene65294.t1 | AT1G63260 | 33.333 | 114 | 72 | 1 | 15 | 128 | 58 | 167 | 3.90e-19 | 79.3 |
| MS.gene65294.t1 | AT1G63260 | 34.211 | 114 | 71 | 1 | 15 | 128 | 58 | 167 | 4.78e-19 | 79.3 |
| MS.gene65294.t1 | AT1G63260 | 33.333 | 114 | 72 | 1 | 15 | 128 | 58 | 167 | 9.22e-19 | 79.3 |
| MS.gene65294.t1 | AT1G63260 | 33.333 | 114 | 72 | 1 | 15 | 128 | 58 | 167 | 1.01e-18 | 79.7 |
| MS.gene65294.t1 | AT1G63260 | 33.333 | 114 | 72 | 1 | 15 | 128 | 58 | 167 | 1.02e-18 | 79.7 |
| MS.gene65294.t1 | AT1G63260 | 33.333 | 114 | 72 | 1 | 15 | 128 | 58 | 167 | 1.02e-18 | 79.7 |
| MS.gene65294.t1 | AT1G18520 | 36.607 | 112 | 68 | 2 | 17 | 128 | 61 | 169 | 1.89e-17 | 76.3 |
| MS.gene65294.t1 | AT2G19580 | 40.351 | 114 | 63 | 2 | 16 | 128 | 58 | 167 | 2.03e-16 | 73.2 |
| MS.gene65294.t1 | AT4G23410 | 33.333 | 114 | 70 | 1 | 15 | 128 | 57 | 164 | 6.10e-16 | 72.0 |
Find 35 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTATGAATAGAGGGTATT+TGG | 0.185250 | 4.3:+41450887 | MS.gene65294:CDS |
| GCTTATGTTGTGACGGATAA+AGG | 0.213333 | 4.3:+41450852 | MS.gene65294:CDS |
| TTAGTCATTGGTGTGCTTAT+TGG | 0.213548 | 4.3:+41450816 | MS.gene65294:CDS |
| GGAAGATTAGTTCTTGTATA+AGG | 0.219711 | 4.3:+41450973 | MS.gene65294:CDS |
| GAAATTCCTATTATGATTAA+TGG | 0.245777 | 4.3:-41450709 | None:intergenic |
| TATTATTTGCAGGATTATTC+TGG | 0.279394 | 4.3:+41450912 | MS.gene65294:CDS |
| AGTGGCTAGTGATGAGTATT+GGG | 0.307047 | 4.3:+41450950 | MS.gene65294:CDS |
| AAGAAAACTCAATCCTATTC+AGG | 0.328328 | 4.3:+41451067 | MS.gene65294:CDS |
| CAATGGCCATTAATCATAAT+AGG | 0.346510 | 4.3:+41450703 | None:intergenic |
| GTATTTGGATTATTATTTGC+AGG | 0.348461 | 4.3:+41450902 | MS.gene65294:CDS |
| GTTGTCATGTTCTTAGTCAT+TGG | 0.349196 | 4.3:+41450804 | MS.gene65294:CDS |
| GAAGATTAGTTCTTGTATAA+GGG | 0.367420 | 4.3:+41450974 | MS.gene65294:CDS |
| ATATAAGAACATACCTGAAT+AGG | 0.397801 | 4.3:-41451080 | None:intergenic |
| CTTATGTTGTGACGGATAAA+GGG | 0.405211 | 4.3:+41450853 | MS.gene65294:CDS |
| CGGATAAAGGGTCTGGAAGA+AGG | 0.448561 | 4.3:+41450865 | MS.gene65294:CDS |
| TTCTAAAGCTTGTAGGAAGT+TGG | 0.451166 | 4.3:+41450998 | MS.gene65294:CDS |
| TAAGGGATTCTAAAGCTTGT+AGG | 0.452393 | 4.3:+41450991 | MS.gene65294:CDS |
| GAGTGGCTAGTGATGAGTAT+TGG | 0.454709 | 4.3:+41450949 | MS.gene65294:CDS |
| CATCATGGTTGTTTCATTAG+CGG | 0.467668 | 4.3:+41450732 | MS.gene65294:CDS |
| GGAAGAAGGGTTATGAATAG+AGG | 0.468654 | 4.3:+41450879 | MS.gene65294:CDS |
| GTTGTGACGGATAAAGGGTC+TGG | 0.489336 | 4.3:+41450858 | MS.gene65294:CDS |
| CGCTAATGAAACAACCATGA+TGG | 0.510097 | 4.3:-41450731 | None:intergenic |
| AAGAACATATCAGCAGTCTC+AGG | 0.515949 | 4.3:-41451041 | None:intergenic |
| ATTTGCAGGATTATTCTGGT+TGG | 0.516447 | 4.3:+41450916 | MS.gene65294:CDS |
| GCAGGATTATTCTGGTTGGT+TGG | 0.518544 | 4.3:+41450920 | MS.gene65294:CDS |
| AATATTTGCTTATGTTGTGA+CGG | 0.520715 | 4.3:+41450845 | MS.gene65294:CDS |
| CATAATAGGAATTTCCATCA+TGG | 0.533378 | 4.3:+41450717 | MS.gene65294:CDS |
| GGATAAAGGGTCTGGAAGAA+GGG | 0.556945 | 4.3:+41450866 | MS.gene65294:CDS |
| GTGGCTAGTGATGAGTATTG+GGG | 0.574200 | 4.3:+41450951 | MS.gene65294:CDS |
| GAAGAAGGGTTATGAATAGA+GGG | 0.576688 | 4.3:+41450880 | MS.gene65294:CDS |
| AAGCTTGTAGGAAGTTGGCT+AGG | 0.591037 | 4.3:+41451003 | MS.gene65294:CDS |
| TGGTTGGTTGGAAGAGAGAG+TGG | 0.610133 | 4.3:+41450932 | MS.gene65294:CDS |
| TGGCTAGTGATGAGTATTGG+GGG | 0.638689 | 4.3:+41450952 | MS.gene65294:CDS |
| AACGTAACAAGAAAGTGTTA+CGG | 0.663996 | 4.3:-41450773 | None:intergenic |
| TGACTAAGAACATGACAACA+AGG | 0.676649 | 4.3:-41450800 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTCCTATTATGATTAA+TGG | - | chr4.3:41450712-41450731 | None:intergenic | 20.0% |
| ! | AATATTTGCTTATGTTGTGA+CGG | + | chr4.3:41450845-41450864 | MS.gene65294:CDS | 25.0% |
| ! | GTATTTGGATTATTATTTGC+AGG | + | chr4.3:41450902-41450921 | MS.gene65294:CDS | 25.0% |
| ! | TATTATTTGCAGGATTATTC+TGG | + | chr4.3:41450912-41450931 | MS.gene65294:CDS | 25.0% |
| !! | GAAGATTAGTTCTTGTATAA+GGG | + | chr4.3:41450974-41450993 | MS.gene65294:CDS | 25.0% |
| CATAATAGGAATTTCCATCA+TGG | + | chr4.3:41450717-41450736 | MS.gene65294:CDS | 30.0% | |
| ! | GGAAGATTAGTTCTTGTATA+AGG | + | chr4.3:41450973-41450992 | MS.gene65294:CDS | 30.0% |
| !! | AACGTAACAAGAAAGTGTTA+CGG | - | chr4.3:41450776-41450795 | None:intergenic | 30.0% |
| ATTTGCAGGATTATTCTGGT+TGG | + | chr4.3:41450916-41450935 | MS.gene65294:CDS | 35.0% | |
| GAAGAAGGGTTATGAATAGA+GGG | + | chr4.3:41450880-41450899 | MS.gene65294:CDS | 35.0% | |
| GTTGTCATGTTCTTAGTCAT+TGG | + | chr4.3:41450804-41450823 | MS.gene65294:CDS | 35.0% | |
| TGACTAAGAACATGACAACA+AGG | - | chr4.3:41450803-41450822 | None:intergenic | 35.0% | |
| TTAGTCATTGGTGTGCTTAT+TGG | + | chr4.3:41450816-41450835 | MS.gene65294:CDS | 35.0% | |
| ! | CATCATGGTTGTTTCATTAG+CGG | + | chr4.3:41450732-41450751 | MS.gene65294:CDS | 35.0% |
| !! | CTTATGTTGTGACGGATAAA+GGG | + | chr4.3:41450853-41450872 | MS.gene65294:CDS | 35.0% |
| !! | GGTTATGAATAGAGGGTATT+TGG | + | chr4.3:41450887-41450906 | MS.gene65294:CDS | 35.0% |
| !! | TAAGGGATTCTAAAGCTTGT+AGG | + | chr4.3:41450991-41451010 | MS.gene65294:CDS | 35.0% |
| !! | TTCTAAAGCTTGTAGGAAGT+TGG | + | chr4.3:41450998-41451017 | MS.gene65294:CDS | 35.0% |
| AAGAACATATCAGCAGTCTC+AGG | - | chr4.3:41451044-41451063 | None:intergenic | 40.0% | |
| AGTGGCTAGTGATGAGTATT+GGG | + | chr4.3:41450950-41450969 | MS.gene65294:CDS | 40.0% | |
| CGCTAATGAAACAACCATGA+TGG | - | chr4.3:41450734-41450753 | None:intergenic | 40.0% | |
| GGAAGAAGGGTTATGAATAG+AGG | + | chr4.3:41450879-41450898 | MS.gene65294:CDS | 40.0% | |
| ! | GTTTCATTAGCGGTTTTGCA+GGG | + | chr4.3:41450742-41450761 | MS.gene65294:CDS | 40.0% |
| ! | TGTTTCATTAGCGGTTTTGC+AGG | + | chr4.3:41450741-41450760 | MS.gene65294:CDS | 40.0% |
| ! | TTTCATTAGCGGTTTTGCAG+GGG | + | chr4.3:41450743-41450762 | MS.gene65294:CDS | 40.0% |
| !! | GCTTATGTTGTGACGGATAA+AGG | + | chr4.3:41450852-41450871 | MS.gene65294:CDS | 40.0% |
| AAGCTTGTAGGAAGTTGGCT+AGG | + | chr4.3:41451003-41451022 | MS.gene65294:CDS | 45.0% | |
| GAGTGGCTAGTGATGAGTAT+TGG | + | chr4.3:41450949-41450968 | MS.gene65294:CDS | 45.0% | |
| GGATAAAGGGTCTGGAAGAA+GGG | + | chr4.3:41450866-41450885 | MS.gene65294:CDS | 45.0% | |
| ! | GCAGGATTATTCTGGTTGGT+TGG | + | chr4.3:41450920-41450939 | MS.gene65294:CDS | 45.0% |
| ! | GTGGCTAGTGATGAGTATTG+GGG | + | chr4.3:41450951-41450970 | MS.gene65294:CDS | 45.0% |
| !! | TGGCTAGTGATGAGTATTGG+GGG | + | chr4.3:41450952-41450971 | MS.gene65294:CDS | 45.0% |
| CGGATAAAGGGTCTGGAAGA+AGG | + | chr4.3:41450865-41450884 | MS.gene65294:CDS | 50.0% | |
| ! | TGGTTGGTTGGAAGAGAGAG+TGG | + | chr4.3:41450932-41450951 | MS.gene65294:CDS | 50.0% |
| !! | GTTGTGACGGATAAAGGGTC+TGG | + | chr4.3:41450858-41450877 | MS.gene65294:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 41450705 | 41451088 | 41450705 | ID=MS.gene65294 |
| chr4.3 | mRNA | 41450705 | 41451088 | 41450705 | ID=MS.gene65294.t1;Parent=MS.gene65294 |
| chr4.3 | exon | 41450705 | 41451088 | 41450705 | ID=MS.gene65294.t1.exon1;Parent=MS.gene65294.t1 |
| chr4.3 | CDS | 41450705 | 41451088 | 41450705 | ID=cds.MS.gene65294.t1;Parent=MS.gene65294.t1 |
| Gene Sequence |
| Protein sequence |
