AlfalfaGEDB | MS.gene66304

M. sativa cultivar XinJiangDaYe / MS.gene66304

KEGG: K01489
GO: GO:0003674 GO:0003824 GO:0004126 GO:0005488 GO:0006139 GO:0006213 GO:0006216 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009116 GO:0009119 GO:0009164 GO:0009972 GO:0009987 GO:0016787 GO:0016810 GO:0016814 GO:0019239 GO:0019439 GO:0034641 GO:0034655 GO:0034656 GO:0042454 GO:0043167 GO:0043169 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044282 GO:0046087 GO:0046131 GO:0046133 GO:0046135 GO:0046483 GO:0046700 GO:0046872 GO:0046914 GO:0055086 GO:0071704 GO:0072527 GO:0072529 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658
PFAM: PF00383.26 PF08211.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66304.t1	XP_003620936.1	96	297	12	0	1	297	1	297	1.80E-155	558.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66304.t1	O65896	56.8	301	118	5	1	292	1	298	1.8e-82	307.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66304.t1	G7KJU9	96.0	297	12	0	1	297	1	297	1.3e-155	558.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene66304	-0.846806	1.72E-59	-1.69E-46
MS.gene050323	MS.gene66304	-0.813701	2.24E-51	-1.69E-46
MS.gene050496	MS.gene66304	-0.82826	9.93E-55	-1.69E-46
MS.gene050800	MS.gene66304	0.826985	2.01E-54	-1.69E-46
MS.gene050868	MS.gene66304	-0.830033	3.69E-55	-1.69E-46
MS.gene050871	MS.gene66304	-0.831052	2.08E-55	-1.69E-46
MS.gene051167	MS.gene66304	0.801233	9.92E-49	-1.69E-46
MS.gene051838	MS.gene66304	-0.803808	2.92E-49	-1.69E-46
MS.gene051946	MS.gene66304	0.801326	9.49E-49	-1.69E-46
MS.gene052164	MS.gene66304	-0.814895	1.22E-51	-1.69E-46
MS.gene05234	MS.gene66304	0.811861	5.67E-51	-1.69E-46
MS.gene052482	MS.gene66304	-0.801336	9.45E-49	-1.69E-46
MS.gene052486	MS.gene66304	-0.809631	1.72E-50	-1.69E-46
MS.gene052503	MS.gene66304	-0.825243	5.21E-54	-1.69E-46
MS.gene052823	MS.gene66304	-0.835605	1.52E-56	-1.69E-46
MS.gene052825	MS.gene66304	-0.837025	6.63E-57	-1.69E-46
MS.gene053203	MS.gene66304	-0.837963	3.81E-57	-1.69E-46
MS.gene053420	MS.gene66304	0.811883	5.60E-51	-1.69E-46
MS.gene053421	MS.gene66304	0.814499	1.49E-51	-1.69E-46
MS.gene053480	MS.gene66304	-0.802889	4.53E-49	-1.69E-46
MS.gene053824	MS.gene66304	-0.818972	1.48E-52	-1.69E-46
MS.gene054003	MS.gene66304	-0.820396	7.01E-53	-1.69E-46
MS.gene054706	MS.gene66304	0.815922	7.21E-52	-1.69E-46
MS.gene054707	MS.gene66304	0.808212	3.46E-50	-1.69E-46
MS.gene055577	MS.gene66304	-0.815925	7.20E-52	-1.69E-46
MS.gene055787	MS.gene66304	0.834409	3.05E-56	-1.69E-46
MS.gene05589	MS.gene66304	-0.817632	2.98E-52	-1.69E-46
MS.gene056046	MS.gene66304	-0.820201	7.77E-53	-1.69E-46
MS.gene056198	MS.gene66304	0.816647	4.97E-52	-1.69E-46
MS.gene056387	MS.gene66304	-0.830036	3.68E-55	-1.69E-46
MS.gene056504	MS.gene66304	-0.828035	1.12E-54	-1.69E-46
MS.gene057014	MS.gene66304	0.820586	6.34E-53	-1.69E-46
MS.gene057173	MS.gene66304	0.844059	9.56E-59	-1.69E-46
MS.gene05726	MS.gene66304	0.860809	1.59E-63	-1.69E-46
MS.gene058157	MS.gene66304	-0.898664	4.25E-77	-1.69E-46
MS.gene05837	MS.gene66304	-0.815458	9.15E-52	-1.69E-46
MS.gene059023	MS.gene66304	-0.802036	6.79E-49	-1.69E-46
MS.gene059223	MS.gene66304	0.831925	1.27E-55	-1.69E-46
MS.gene059699	MS.gene66304	0.808585	2.88E-50	-1.69E-46
MS.gene059703	MS.gene66304	0.827406	1.59E-54	-1.69E-46
MS.gene059718	MS.gene66304	-0.805673	1.19E-49	-1.69E-46
MS.gene05995	MS.gene66304	-0.841571	4.38E-58	-1.69E-46
MS.gene05996	MS.gene66304	-0.816831	4.51E-52	-1.69E-46
MS.gene06000	MS.gene66304	-0.814614	1.41E-51	-1.69E-46
MS.gene060187	MS.gene66304	0.815237	1.02E-51	-1.69E-46
MS.gene060595	MS.gene66304	0.835987	1.22E-56	-1.69E-46
MS.gene060778	MS.gene66304	-0.821233	4.50E-53	-1.69E-46
MS.gene06077	MS.gene66304	-0.81836	2.04E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene66304	MS.gene06119	PPI
MS.gene66304	MS.gene20200	PPI
MS.gene66304	MS.gene55838	PPI
MS.gene66304	MS.gene048972	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66304.t1	MTR_6g092660	95.960	297	12	0	1	297	1	297	0.0	582
MS.gene66304.t1	MTR_8g101570	60.410	293	108	3	1	292	1	286	1.06e-121	350

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66304.t1	AT2G19570	55.814	301	121	4	1	292	1	298	1.66e-108	317
MS.gene66304.t1	AT4G29610	50.719	278	125	5	28	298	21	293	1.39e-85	259
MS.gene66304.t1	AT4G29600	48.658	298	134	6	5	292	8	296	2.25e-82	251
MS.gene66304.t1	AT4G29620	45.791	297	138	5	5	293	29	310	5.90e-75	233
MS.gene66304.t1	AT4G29570	44.625	307	140	11	1	292	1	292	4.62e-63	201
MS.gene66304.t1	AT4G29630	48.230	226	103	3	2	219	4	223	4.84e-59	188
MS.gene66304.t1	AT4G29640	41.613	310	155	11	2	298	3	299	2.03e-56	186
MS.gene66304.t1	AT4G29580	43.657	268	131	8	27	282	27	286	3.11e-50	168
MS.gene66304.t1	AT4G29580	43.657	268	131	8	27	282	27	286	6.23e-49	169
MS.gene66304.t1	AT4G29650	39.041	292	128	8	2	292	3	245	2.58e-47	159

Find 98 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCTTCAGGTGTGGCTTTAC+TGG	0.180402	6.4:+63602041	MS.gene66304:CDS
GATGCTTCTATTATGAATTT+AGG	0.212943	6.4:-63601442	None:intergenic
GAGTTTCTTTGTTATGAAAA+AGG	0.248603	6.4:-63601687	None:intergenic
TTAGGGTTAGGTTTGTTGAT+TGG	0.275993	6.4:-63601483	None:intergenic
TAGGGTTAGGTTTGTTGATT+GGG	0.297609	6.4:-63601482	None:intergenic
AAGAGAAGAGGAACATGTTT+AGG	0.300564	6.4:-63601892	None:intergenic
TGATGGGAAGGAGTTGGGTT+AGG	0.306745	6.4:-63601501	None:intergenic
TCCTAGCATGGGACCGGTTC+AGG	0.312849	6.4:+63602116	MS.gene66304:CDS
GACGGCGGCAACGTGAAATT+TGG	0.324323	6.4:-63601563	None:intergenic
CTTTCAGTGCTGCATCCTTC+AGG	0.328078	6.4:-63601984	None:intergenic
ATACGGCAAGGCTGTTTCTT+AGG	0.332593	6.4:+63602247	MS.gene66304:CDS
TGTTTATAAAGGTAGTTATA+TGG	0.339228	6.4:+63602077	MS.gene66304:CDS
GAATTCCTTTCTCATCCCTT+TGG	0.345512	6.4:+63601853	MS.gene66304:CDS
GACAATGATGGGAAGGAGTT+GGG	0.351225	6.4:-63601506	None:intergenic
GGAGACGGTGCGGCGGAGTA+TGG	0.354349	6.4:+63602168	MS.gene66304:CDS
GGGACCGGTTCAGGCTGCTT+TGG	0.365571	6.4:+63602125	MS.gene66304:CDS
CACGGATTTCTTGAAGAAAC+TGG	0.368852	6.4:-63601756	None:intergenic
GAAGGAGTTGGGTTAGGGTT+AGG	0.374079	6.4:-63601495	None:intergenic
GCAACGTGAAATTTGGAGAT+AGG	0.374243	6.4:-63601556	None:intergenic
CCGTACAGTAACTCGCCTTC+AGG	0.374556	6.4:+63602027	MS.gene66304:CDS
AGAGGATGCGGTGGTGAAAC+AGG	0.378180	6.4:+63602221	MS.gene66304:CDS
TAAACATGTTCCTCTTCTCT+TGG	0.383408	6.4:+63601894	MS.gene66304:CDS
GATGGGAAGGAGTTGGGTTA+GGG	0.393643	6.4:-63601500	None:intergenic
CACGTTGCCGCCGTCGCTGT+TGG	0.395330	6.4:+63601571	MS.gene66304:CDS
TCACGTGGGAATGTTTATAA+AGG	0.399839	6.4:+63602066	MS.gene66304:CDS
GCCACCAAAGCAGCCTGAAC+CGG	0.415674	6.4:-63602129	None:intergenic
ACCGGTTCAGGCTGCTTTGG+TGG	0.418994	6.4:+63602128	MS.gene66304:CDS
TGACAATGATGGGAAGGAGT+TGG	0.424779	6.4:-63601507	None:intergenic
TGCGGCGGAGTATGGAGAAT+TGG	0.433354	6.4:+63602176	MS.gene66304:CDS
GTATGGTGGAAAGGAAGGTT+AGG	0.441709	6.4:-63601634	None:intergenic
GAGATAGGGACACGGGCTAA+TGG	0.450344	6.4:-63601541	None:intergenic
AGGGACACGGGCTAATGGTT+GGG	0.455742	6.4:-63601536	None:intergenic
CTTGAAGAAACTGGCGGCAA+TGG	0.468441	6.4:-63601747	None:intergenic
GCCGTCGCTGTTGGAATCTC+CGG	0.472457	6.4:+63601580	MS.gene66304:CDS
TTTATTGTCGGTGCCGGAGA+CGG	0.475580	6.4:+63602153	MS.gene66304:CDS
AGAATTGGTGGAAGCGGTGT+TGG	0.478163	6.4:+63602191	MS.gene66304:CDS
GGATGGTATGGTGGAAAGGA+AGG	0.479635	6.4:-63601639	None:intergenic
TTGGGATGATTTGACAATGA+TGG	0.485433	6.4:-63601518	None:intergenic
TAGGGACACGGGCTAATGGT+TGG	0.490242	6.4:-63601537	None:intergenic
TCTTCGAATCAGATGTTATG+AGG	0.491889	6.4:-63601798	None:intergenic
ATACTCCGCCGCACCGTCTC+CGG	0.496985	6.4:-63602166	None:intergenic
CTTCAAGAAATCCGTGGCGC+CGG	0.503869	6.4:+63601763	MS.gene66304:CDS
ATTGGTGGAAGCGGTGTTGG+TGG	0.506203	6.4:+63602194	MS.gene66304:CDS
TTCAATAAAGCCACACCTGA+AGG	0.511344	6.4:+63601969	MS.gene66304:CDS
TGCAGCTTATAATCCTAGCA+TGG	0.518529	6.4:+63602104	MS.gene66304:CDS
AGTGCTGCATCCTTCAGGTG+TGG	0.533445	6.4:-63601979	None:intergenic
TGGGATGATTTGACAATGAT+GGG	0.533779	6.4:-63601517	None:intergenic
GAGAAACTGTTCAGCATGGA+TGG	0.539294	6.4:-63601656	None:intergenic
GCCTGAACCGGTCCCATGCT+AGG	0.541079	6.4:-63602117	None:intergenic
TATGGTGGAAAGGAAGGTTA+GGG	0.550775	6.4:-63601633	None:intergenic
AGAAGATCATGAGGACCAAA+GGG	0.550838	6.4:-63601868	None:intergenic
AAGAAGATCATGAGGACCAA+AGG	0.551279	6.4:-63601869	None:intergenic
TCCAGTAAAGCCACACCTGA+AGG	0.553848	6.4:-63602042	None:intergenic
CAACGTGAAATTTGGAGATA+GGG	0.554542	6.4:-63601555	None:intergenic
ACTGTTCAGCATGGATGGTA+TGG	0.559714	6.4:-63601651	None:intergenic
GAGGACCAAAGGGATGAGAA+AGG	0.560227	6.4:-63601858	None:intergenic
AGCGGTGTTGGTGGAGAAAG+AGG	0.561960	6.4:+63602203	MS.gene66304:CDS
CAGTAACTCGCCTTCAGGTG+TGG	0.564454	6.4:+63602032	MS.gene66304:CDS
GGTGGTGAAACAGGAGGATA+CGG	0.564967	6.4:+63602230	MS.gene66304:CDS
GCATGGATGGTATGGTGGAA+AGG	0.568653	6.4:-63601643	None:intergenic
AACTGGCGGCAATGGCCACA+AGG	0.571846	6.4:-63601739	None:intergenic
TTATAATCCTAGCATGGGAC+CGG	0.572239	6.4:+63602110	MS.gene66304:CDS
GTATGGAGAATTGGTGGAAG+CGG	0.578039	6.4:+63602185	MS.gene66304:CDS
GAAGCTACATTGAGGTGCGA+TGG	0.586638	6.4:-63602271	None:intergenic
TTAAGAGAAACTGTTCAGCA+TGG	0.588008	6.4:-63601660	None:intergenic
GAAGGATGCAGCACTGAAAG+CGG	0.589558	6.4:+63601987	MS.gene66304:CDS
ATCTCCGGTCGAATCTTCAT+CGG	0.594505	6.4:+63601595	MS.gene66304:CDS
GAGGAATATCTCCGGCGCCA+CGG	0.594736	6.4:-63601774	None:intergenic
GGCGGAGTATGGAGAATTGG+TGG	0.610347	6.4:+63602179	MS.gene66304:CDS
CAGTTTCTTCAAGAAATCCG+TGG	0.610963	6.4:+63601757	MS.gene66304:CDS
GTTGGTGGAGAAAGAGGATG+CGG	0.610963	6.4:+63602209	MS.gene66304:CDS
TGTCGGTGCCGGAGACGGTG+CGG	0.611623	6.4:+63602158	MS.gene66304:CDS
ACCGGAGATTCCAACAGCGA+CGG	0.615346	6.4:-63601581	None:intergenic
GCAGCTTATAATCCTAGCAT+GGG	0.617961	6.4:+63602105	MS.gene66304:CDS
GGAGATTCCAACAGCGACGG+CGG	0.619242	6.4:-63601578	None:intergenic
GCTGTATCCGCTGCACCTTG+TGG	0.624321	6.4:+63601724	MS.gene66304:CDS
TGACGTGGTTCCAAGAGAAG+AGG	0.624352	6.4:-63601904	None:intergenic
TATGAGGAGAGGAATATCTC+CGG	0.625052	6.4:-63601782	None:intergenic
GACGCCGATGAAGATTCGAC+CGG	0.625190	6.4:-63601599	None:intergenic
ATGATTTGACAATGATGGGA+AGG	0.628836	6.4:-63601513	None:intergenic
CGGTGCCGGAGACGGTGCGG+CGG	0.633103	6.4:+63602161	MS.gene66304:CDS
CCTGAAGGCGAGTTACTGTA+CGG	0.638330	6.4:-63602027	None:intergenic
GAATCAGATGTTATGAGGAG+AGG	0.641146	6.4:-63601793	None:intergenic
GCAATGGCCACAAGGTGCAG+CGG	0.645064	6.4:-63601731	None:intergenic
GTTCAGCATGGATGGTATGG+TGG	0.647203	6.4:-63601648	None:intergenic
AAATTTGGAGATAGGGACAC+GGG	0.647299	6.4:-63601548	None:intergenic
GTGGCTTTACTGGATTCACG+TGG	0.658001	6.4:+63602051	MS.gene66304:CDS
TGTTTAGGAAGAAGATCATG+AGG	0.661557	6.4:-63601877	None:intergenic
ACCACATTGAAGCTACATTG+AGG	0.663804	6.4:-63602279	None:intergenic
AAGGTGCAGCGGATACAGCA+AGG	0.664429	6.4:-63601720	None:intergenic
GAAATTTGGAGATAGGGACA+CGG	0.671783	6.4:-63601549	None:intergenic
GGATTTCTTGAAGAAACTGG+CGG	0.674203	6.4:-63601753	None:intergenic
TGGCTTTACTGGATTCACGT+GGG	0.675090	6.4:+63602052	MS.gene66304:CDS
GGTGGAGAAAGAGGATGCGG+TGG	0.682018	6.4:+63602212	MS.gene66304:CDS
GGATGCGGTGGTGAAACAGG+AGG	0.700486	6.4:+63602224	MS.gene66304:CDS
ACCTCAATGTAGCTTCAATG+TGG	0.704261	6.4:+63602278	MS.gene66304:CDS
AAACACAAATCATTGTGACG+TGG	0.715133	6.4:-63601919	None:intergenic
TGAAACAGGAGGATACGGCA+AGG	0.721213	6.4:+63602235	MS.gene66304:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTTTATAAAGGTAGTTATA+TGG	+	chr6.4:63602077-63602096	MS.gene66304:CDS	20.0%
!!!	TTGTGTTTTATAGAAATAGA+AGG	+	chr6.4:63601934-63601953	MS.gene66304:CDS	20.0%
!	GATGCTTCTATTATGAATTT+AGG	-	chr6.4:63601445-63601464	None:intergenic	25.0%
!!	GAGTTTCTTTGTTATGAAAA+AGG	-	chr6.4:63601690-63601709	None:intergenic	25.0%
	AAACACAAATCATTGTGACG+TGG	-	chr6.4:63601922-63601941	None:intergenic	35.0%
	AAGAGAAGAGGAACATGTTT+AGG	-	chr6.4:63601895-63601914	None:intergenic	35.0%
	CAACGTGAAATTTGGAGATA+GGG	-	chr6.4:63601558-63601577	None:intergenic	35.0%
	TAAACATGTTCCTCTTCTCT+TGG	+	chr6.4:63601894-63601913	MS.gene66304:CDS	35.0%
	TCACGTGGGAATGTTTATAA+AGG	+	chr6.4:63602066-63602085	MS.gene66304:CDS	35.0%
	TCTTCGAATCAGATGTTATG+AGG	-	chr6.4:63601801-63601820	None:intergenic	35.0%
	TGGGATGATTTGACAATGAT+GGG	-	chr6.4:63601520-63601539	None:intergenic	35.0%
	TGTTTAGGAAGAAGATCATG+AGG	-	chr6.4:63601880-63601899	None:intergenic	35.0%
	TTAAGAGAAACTGTTCAGCA+TGG	-	chr6.4:63601663-63601682	None:intergenic	35.0%
	TTGGGATGATTTGACAATGA+TGG	-	chr6.4:63601521-63601540	None:intergenic	35.0%
!	ATGATTTGACAATGATGGGA+AGG	-	chr6.4:63601516-63601535	None:intergenic	35.0%
!!	TAGGGTTAGGTTTGTTGATT+GGG	-	chr6.4:63601485-63601504	None:intergenic	35.0%
!!	TTAGGGTTAGGTTTGTTGAT+TGG	-	chr6.4:63601486-63601505	None:intergenic	35.0%
	AAATTTGGAGATAGGGACAC+GGG	-	chr6.4:63601551-63601570	None:intergenic	40.0%
	AAGAAGATCATGAGGACCAA+AGG	-	chr6.4:63601872-63601891	None:intergenic	40.0%
	ACCACATTGAAGCTACATTG+AGG	-	chr6.4:63602282-63602301	None:intergenic	40.0%
	ACCTCAATGTAGCTTCAATG+TGG	+	chr6.4:63602278-63602297	MS.gene66304:CDS	40.0%
	AGAAGATCATGAGGACCAAA+GGG	-	chr6.4:63601871-63601890	None:intergenic	40.0%
	CACGGATTTCTTGAAGAAAC+TGG	-	chr6.4:63601759-63601778	None:intergenic	40.0%
	CAGTTTCTTCAAGAAATCCG+TGG	+	chr6.4:63601757-63601776	MS.gene66304:CDS	40.0%
	GAAATTTGGAGATAGGGACA+CGG	-	chr6.4:63601552-63601571	None:intergenic	40.0%
	GAATCAGATGTTATGAGGAG+AGG	-	chr6.4:63601796-63601815	None:intergenic	40.0%
	GAATTCCTTTCTCATCCCTT+TGG	+	chr6.4:63601853-63601872	MS.gene66304:CDS	40.0%
	GCAACGTGAAATTTGGAGAT+AGG	-	chr6.4:63601559-63601578	None:intergenic	40.0%
	GCAGCTTATAATCCTAGCAT+GGG	+	chr6.4:63602105-63602124	MS.gene66304:CDS	40.0%
	GGATTTCTTGAAGAAACTGG+CGG	-	chr6.4:63601756-63601775	None:intergenic	40.0%
	TATGAGGAGAGGAATATCTC+CGG	-	chr6.4:63601785-63601804	None:intergenic	40.0%
	TGCAGCTTATAATCCTAGCA+TGG	+	chr6.4:63602104-63602123	MS.gene66304:CDS	40.0%
	TTATAATCCTAGCATGGGAC+CGG	+	chr6.4:63602110-63602129	MS.gene66304:CDS	40.0%
	TTCAATAAAGCCACACCTGA+AGG	+	chr6.4:63601969-63601988	MS.gene66304:CDS	40.0%
!	TATGGTGGAAAGGAAGGTTA+GGG	-	chr6.4:63601636-63601655	None:intergenic	40.0%
!!	GCTTTGGTGGCTTTTATTGT+CGG	+	chr6.4:63602141-63602160	MS.gene66304:CDS	40.0%
	ACTGTTCAGCATGGATGGTA+TGG	-	chr6.4:63601654-63601673	None:intergenic	45.0%
	ATCTCCGGTCGAATCTTCAT+CGG	+	chr6.4:63601595-63601614	MS.gene66304:CDS	45.0%
	GAGAAACTGTTCAGCATGGA+TGG	-	chr6.4:63601659-63601678	None:intergenic	45.0%
	GTATGGAGAATTGGTGGAAG+CGG	+	chr6.4:63602185-63602204	MS.gene66304:CDS	45.0%
!	GACAATGATGGGAAGGAGTT+GGG	-	chr6.4:63601509-63601528	None:intergenic	45.0%
!	GTATGGTGGAAAGGAAGGTT+AGG	-	chr6.4:63601637-63601656	None:intergenic	45.0%
!	TGACAATGATGGGAAGGAGT+TGG	-	chr6.4:63601510-63601529	None:intergenic	45.0%
!	TGGCTTTACTGGATTCACGT+GGG	+	chr6.4:63602052-63602071	MS.gene66304:CDS	45.0%
!!	ATACGGCAAGGCTGTTTCTT+AGG	+	chr6.4:63602247-63602266	MS.gene66304:CDS	45.0%
	AGAATTGGTGGAAGCGGTGT+TGG	+	chr6.4:63602191-63602210	MS.gene66304:CDS	50.0%
	CCTGAAGGCGAGTTACTGTA+CGG	-	chr6.4:63602030-63602049	None:intergenic	50.0%
	CTTGAAGAAACTGGCGGCAA+TGG	-	chr6.4:63601750-63601769	None:intergenic	50.0%
	CTTTCAGTGCTGCATCCTTC+AGG	-	chr6.4:63601987-63602006	None:intergenic	50.0%
	GAAGCTACATTGAGGTGCGA+TGG	-	chr6.4:63602274-63602293	None:intergenic	50.0%
	GAGGACCAAAGGGATGAGAA+AGG	-	chr6.4:63601861-63601880	None:intergenic	50.0%
	GATGGGAAGGAGTTGGGTTA+GGG	-	chr6.4:63601503-63601522	None:intergenic	50.0%
	GGTGGTGAAACAGGAGGATA+CGG	+	chr6.4:63602230-63602249	MS.gene66304:CDS	50.0%
	GTTCAGCATGGATGGTATGG+TGG	-	chr6.4:63601651-63601670	None:intergenic	50.0%
	TCCAGTAAAGCCACACCTGA+AGG	-	chr6.4:63602045-63602064	None:intergenic	50.0%
	TGAAACAGGAGGATACGGCA+AGG	+	chr6.4:63602235-63602254	MS.gene66304:CDS	50.0%
	TGACGTGGTTCCAAGAGAAG+AGG	-	chr6.4:63601907-63601926	None:intergenic	50.0%
	TGATGGGAAGGAGTTGGGTT+AGG	-	chr6.4:63601504-63601523	None:intergenic	50.0%
!	GAAGGAGTTGGGTTAGGGTT+AGG	-	chr6.4:63601498-63601517	None:intergenic	50.0%
!	GAAGGATGCAGCACTGAAAG+CGG	+	chr6.4:63601987-63602006	MS.gene66304:CDS	50.0%
!	GCATGGATGGTATGGTGGAA+AGG	-	chr6.4:63601646-63601665	None:intergenic	50.0%
!	GGATGGTATGGTGGAAAGGA+AGG	-	chr6.4:63601642-63601661	None:intergenic	50.0%
!	GTGGCTTTACTGGATTCACG+TGG	+	chr6.4:63602051-63602070	MS.gene66304:CDS	50.0%
!	GTGGCTTTTATTGTCGGTGC+CGG	+	chr6.4:63602147-63602166	MS.gene66304:CDS	50.0%
!	GTTGGTGGAGAAAGAGGATG+CGG	+	chr6.4:63602209-63602228	MS.gene66304:CDS	50.0%
!!	TTTATTGTCGGTGCCGGAGA+CGG	+	chr6.4:63602153-63602172	MS.gene66304:CDS	50.0%
	AAGGTGCAGCGGATACAGCA+AGG	-	chr6.4:63601723-63601742	None:intergenic	55.0%
	ACCGGAGATTCCAACAGCGA+CGG	-	chr6.4:63601584-63601603	None:intergenic	55.0%
	AGAGGATGCGGTGGTGAAAC+AGG	+	chr6.4:63602221-63602240	MS.gene66304:CDS	55.0%
	AGTGCTGCATCCTTCAGGTG+TGG	-	chr6.4:63601982-63602001	None:intergenic	55.0%
	CAGTAACTCGCCTTCAGGTG+TGG	+	chr6.4:63602032-63602051	MS.gene66304:CDS	55.0%
	CCGTACAGTAACTCGCCTTC+AGG	+	chr6.4:63602027-63602046	MS.gene66304:CDS	55.0%
	CTTCAAGAAATCCGTGGCGC+CGG	+	chr6.4:63601763-63601782	MS.gene66304:CDS	55.0%
	GACGCCGATGAAGATTCGAC+CGG	-	chr6.4:63601602-63601621	None:intergenic	55.0%
	GACGGCGGCAACGTGAAATT+TGG	-	chr6.4:63601566-63601585	None:intergenic	55.0%
	GGCGGAGTATGGAGAATTGG+TGG	+	chr6.4:63602179-63602198	MS.gene66304:CDS	55.0%
	TGCGGCGGAGTATGGAGAAT+TGG	+	chr6.4:63602176-63602195	MS.gene66304:CDS	55.0%
!	AGGGACACGGGCTAATGGTT+GGG	-	chr6.4:63601539-63601558	None:intergenic	55.0%
!	ATTGGTGGAAGCGGTGTTGG+TGG	+	chr6.4:63602194-63602213	MS.gene66304:CDS	55.0%
!	GAGATAGGGACACGGGCTAA+TGG	-	chr6.4:63601544-63601563	None:intergenic	55.0%
!	GCCTTCAGGTGTGGCTTTAC+TGG	+	chr6.4:63602041-63602060	MS.gene66304:CDS	55.0%
!	TAGGGACACGGGCTAATGGT+TGG	-	chr6.4:63601540-63601559	None:intergenic	55.0%
!!	AGCGGTGTTGGTGGAGAAAG+AGG	+	chr6.4:63602203-63602222	MS.gene66304:CDS	55.0%
	AACTGGCGGCAATGGCCACA+AGG	-	chr6.4:63601742-63601761	None:intergenic	60.0%
	GAGGAATATCTCCGGCGCCA+CGG	-	chr6.4:63601777-63601796	None:intergenic	60.0%
	GCAATGGCCACAAGGTGCAG+CGG	-	chr6.4:63601734-63601753	None:intergenic	60.0%
	GCCACCAAAGCAGCCTGAAC+CGG	-	chr6.4:63602132-63602151	None:intergenic	60.0%
	GCCGTCGCTGTTGGAATCTC+CGG	+	chr6.4:63601580-63601599	MS.gene66304:CDS	60.0%
	GCTGTATCCGCTGCACCTTG+TGG	+	chr6.4:63601724-63601743	MS.gene66304:CDS	60.0%
	GGAGATTCCAACAGCGACGG+CGG	-	chr6.4:63601581-63601600	None:intergenic	60.0%
	GGATGCGGTGGTGAAACAGG+AGG	+	chr6.4:63602224-63602243	MS.gene66304:CDS	60.0%
	GGTGGAGAAAGAGGATGCGG+TGG	+	chr6.4:63602212-63602231	MS.gene66304:CDS	60.0%
!	ACCGGTTCAGGCTGCTTTGG+TGG	+	chr6.4:63602128-63602147	MS.gene66304:CDS	60.0%
!	TCCTAGCATGGGACCGGTTC+AGG	+	chr6.4:63602116-63602135	MS.gene66304:CDS	60.0%
	ATACTCCGCCGCACCGTCTC+CGG	-	chr6.4:63602169-63602188	None:intergenic	65.0%
	GCCTGAACCGGTCCCATGCT+AGG	-	chr6.4:63602120-63602139	None:intergenic	65.0%
!	GGGACCGGTTCAGGCTGCTT+TGG	+	chr6.4:63602125-63602144	MS.gene66304:CDS	65.0%
	CACGTTGCCGCCGTCGCTGT+TGG	+	chr6.4:63601571-63601590	MS.gene66304:CDS	70.0%
	GGAGACGGTGCGGCGGAGTA+TGG	+	chr6.4:63602168-63602187	MS.gene66304:CDS	70.0%
!!	TGTCGGTGCCGGAGACGGTG+CGG	+	chr6.4:63602158-63602177	MS.gene66304:CDS	70.0%
!!	CGGTGCCGGAGACGGTGCGG+CGG	+	chr6.4:63602161-63602180	MS.gene66304:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	63601433	63602332	63601433	ID=MS.gene66304
chr6.4	mRNA	63601433	63602332	63601433	ID=MS.gene66304.t1;Parent=MS.gene66304
chr6.4	exon	63601433	63602332	63601433	ID=MS.gene66304.t1.exon1;Parent=MS.gene66304.t1
chr6.4	CDS	63601433	63602332	63601433	ID=cds.MS.gene66304.t1;Parent=MS.gene66304.t1

Gene Sequence

>MS.gene66304

ATGGAAGAACCTAAATTCATAATAGAAGCATCAGAAGCAAAATCATTAACCCAATCAACAAACCTAACCCTAACCCAACTCCTTCCCATCATTGTCAAATCATCCCAACCATTAGCCCGTGTCCCTATCTCCAAATTTCACGTTGCCGCCGTCGCTGTTGGAATCTCCGGTCGAATCTTCATCGGCGTCAACGTCGAATTCCCTAACCTTCCTTTCCACCATACCATCCATGCTGAACAGTTTCTCTTAACAAACCTTTTTCATAACAAAGAAACTCAAATTCAATACCTTGCTGTATCCGCTGCACCTTGTGGCCATTGCCGCCAGTTTCTTCAAGAAATCCGTGGCGCCGGAGATATTCCTCTCCTCATAACATCTGATTCGAAGAAAACTGCGAAAAATGAGTTTACATCTCTTTCTGAATTCCTTTCTCATCCCTTTGGTCCTCATGATCTTCTTCCTAAACATGTTCCTCTTCTCTTGGAACCACGTCACAATGATTTGTGTTTTATAGAAATAGAAGGTTCGATCACAAATTCAATAAAGCCACACCTGAAGGATGCAGCACTGAAAGCGGCGAATGAATCACATGCGCCGTACAGTAACTCGCCTTCAGGTGTGGCTTTACTGGATTCACGTGGGAATGTTTATAAAGGTAGTTATATGGAATCTGCAGCTTATAATCCTAGCATGGGACCGGTTCAGGCTGCTTTGGTGGCTTTTATTGTCGGTGCCGGAGACGGTGCGGCGGAGTATGGAGAATTGGTGGAAGCGGTGTTGGTGGAGAAAGAGGATGCGGTGGTGAAACAGGAGGATACGGCAAGGCTGTTTCTTAGGTCCATCGCACCTCAATGTAGCTTCAATGTGGTTCTTTGCACTTCTAATAATAAATCTAATTGA

Protein sequence

>MS.gene66304.t1

MEEPKFIIEASEAKSLTQSTNLTLTQLLPIIVKSSQPLARVPISKFHVAAVAVGISGRIFIGVNVEFPNLPFHHTIHAEQFLLTNLFHNKETQIQYLAVSAAPCGHCRQFLQEIRGAGDIPLLITSDSKKTAKNEFTSLSEFLSHPFGPHDLLPKHVPLLLEPRHNDLCFIEIEGSITNSIKPHLKDAALKAANESHAPYSNSPSGVALLDSRGNVYKGSYMESAAYNPSMGPVQAALVAFIVGAGDGAAEYGELVEAVLVEKEDAVVKQEDTARLFLRSIAPQCSFNVVLCTSNNKSN