AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75477


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75477.t1 MTR_3g087190 97.115 208 6 0 1 208 1 208 1.44e-148 411
MS.gene75477.t1 MTR_3g079680 66.026 156 21 3 16 170 27 151 8.47e-59 182
MS.gene75477.t1 MTR_8g090225 56.790 81 33 2 20 99 13 92 8.05e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75477.t1 AT4G39880 72.727 132 34 1 1 132 1 130 1.81e-61 189

Find 67 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGGGTTTAAGCCGCAAAA+TGG 0.269582 3.3:-72716331 MS.gene75477:CDS
TCGAATCTGTTTGCGAGTTC+TGG 0.270288 3.3:-72716277 MS.gene75477:CDS
TGCGAGTTCTGGTTCGAATC+AGG 0.295567 3.3:-72716266 MS.gene75477:CDS
TGCAAAACCTGATTACAAAA+AGG 0.295568 3.3:-72716515 MS.gene75477:CDS
AGCCGCAAAATGGGTATTAC+AGG 0.309367 3.3:-72716321 MS.gene75477:CDS
AACACGCTTGATTTCAATCT+TGG 0.311058 3.3:+72716624 None:intergenic
CAGGGACGGTTTGACCGTTC+TGG 0.311213 3.3:-72716247 MS.gene75477:CDS
GATTGAAATCAAGCGTGTTC+TGG 0.327858 3.3:-72716620 MS.gene75477:CDS
TTGAACGCCCTGGTTTGAAC+AGG 0.371974 3.3:-72716207 MS.gene75477:CDS
TAAGAAGAGTTTGAATAAAC+AGG 0.389935 3.3:-72716422 MS.gene75477:CDS
AGGGGAGGGTTTGATCGTTC+TGG 0.399564 3.3:-72716301 MS.gene75477:CDS
TTGACCGTTCTGGTTCGAAC+CGG 0.419451 3.3:-72716237 MS.gene75477:CDS
AAGCGCCGGGGAAGAGTCAT+TGG 0.426778 3.3:-72716375 MS.gene75477:CDS
ATTTCAATCTTGGAAGCTGA+TGG 0.434077 3.3:+72716634 None:intergenic
GCGAGTTCTGGTTCGAATCA+GGG 0.449121 3.3:-72716265 MS.gene75477:CDS
TGTATGTCGGTGAAGGTTGT+AGG 0.452936 3.3:+72716679 None:intergenic
GTAGGCAGGAGAAGCTTGAT+TGG 0.456166 3.3:+72716697 None:intergenic
ACCAGTCCTCATGTTACTCC+AGG 0.456222 3.3:+72716159 None:intergenic
GTTCTGCTGCAAAGTTTCCC+TGG 0.467776 3.3:-72716177 MS.gene75477:CDS
TGACCGTTCTGGTTCGAACC+GGG 0.469581 3.3:-72716236 MS.gene75477:CDS
TCCTCTTCAATCTCTCGAAT+CGG 0.471084 3.3:+72716445 None:intergenic
AGTATTGTTGAGGAAGCGCC+GGG 0.487009 3.3:-72716388 MS.gene75477:CDS
CCATCAGCCCTGTTCAAACC+AGG 0.499539 3.3:+72716199 None:intergenic
GCAGGAGAAGCTTGATTGGT+AGG 0.500966 3.3:+72716701 None:intergenic
GATTCGGGTTAATCAAAAGC+GGG 0.502463 3.3:+72716473 None:intergenic
CGGGGAAGGTTTGAACGCCC+TGG 0.503685 3.3:-72716217 MS.gene75477:CDS
GTGGGTTTAAGCCGCAAAAT+GGG 0.507177 3.3:-72716330 MS.gene75477:CDS
CTACGGATTCGATGTCGAAA+AGG 0.508089 3.3:-72716590 MS.gene75477:CDS
AATCTTGGAAGCTGATGGAA+TGG 0.521693 3.3:+72716639 None:intergenic
CCTGGTTTGAACAGGGCTGA+TGG 0.524674 3.3:-72716199 MS.gene75477:CDS
CTGGTAGGAGTAATGCTACT+AGG 0.531882 3.3:-72716141 MS.gene75477:CDS
GAGTTTGAATAAACAGGCTA+TGG 0.532603 3.3:-72716416 MS.gene75477:CDS
TTCGACATCGAATCCGTAGA+GGG 0.537808 3.3:+72716594 None:intergenic
CAGGGCGTTCAAACCTTCCC+CGG 0.541271 3.3:+72716218 None:intergenic
CCCTGGAGTAACATGAGGAC+TGG 0.545437 3.3:-72716160 MS.gene75477:CDS
TAGTATTGTTGAGGAAGCGC+CGG 0.547147 3.3:-72716389 MS.gene75477:CDS
CGTGTTCTGGAATCCCTCTA+CGG 0.549464 3.3:-72716607 MS.gene75477:CDS
GTTCTGGTTCGAACCGGGGA+AGG 0.563380 3.3:-72716231 MS.gene75477:CDS
GATTTCATGTATGTCGGTGA+AGG 0.563490 3.3:+72716672 None:intergenic
TGTCGGTGAAGGTTGTAGGC+AGG 0.564653 3.3:+72716683 None:intergenic
GGAGTAACATGAGGACTGGT+AGG 0.575261 3.3:-72716156 MS.gene75477:CDS
TGAACGCCCTGGTTTGAACA+GGG 0.577798 3.3:-72716206 MS.gene75477:CDS
AAAATGGGTATTACAGGGGA+GGG 0.587136 3.3:-72716315 MS.gene75477:CDS
GAGAGCGATTTCATGTATGT+CGG 0.587586 3.3:+72716666 None:intergenic
GCCGCAAAATGGGTATTACA+GGG 0.593489 3.3:-72716320 MS.gene75477:CDS
GACCGTTCTGGTTCGAACCG+GGG 0.600430 3.3:-72716235 MS.gene75477:CDS
TCCGATTCGAGAGATTGAAG+AGG 0.601664 3.3:-72716446 MS.gene75477:CDS
CAAAATGGGTATTACAGGGG+AGG 0.606255 3.3:-72716316 MS.gene75477:CDS
TTTGAATAAACAGGCTATGG+CGG 0.615531 3.3:-72716413 MS.gene75477:CDS
GATGAGAATAAGAACACTCG+TGG 0.616080 3.3:-72716349 MS.gene75477:CDS
TCAAGCCAATGACTCTTCCC+CGG 0.618018 3.3:+72716370 None:intergenic
GTTCTGGTTCGAATCAGGGA+CGG 0.619690 3.3:-72716261 MS.gene75477:CDS
GTTCGAACATTGAACATGGA+CGG 0.633586 3.3:-72716568 MS.gene75477:CDS
TTTCGACATCGAATCCGTAG+AGG 0.637992 3.3:+72716593 None:intergenic
CCAGTCCTCATGTTACTCCA+GGG 0.639983 3.3:+72716160 None:intergenic
AAAGGTTCGAACATTGAACA+TGG 0.645998 3.3:-72716572 MS.gene75477:CDS
ATGAGAATAAGAACACTCGT+GGG 0.659603 3.3:-72716348 MS.gene75477:CDS
TATGGCGGCTAGTATTGTTG+AGG 0.660904 3.3:-72716398 MS.gene75477:CDS
AGTTTCCCTGGAGTAACATG+AGG 0.671157 3.3:-72716165 MS.gene75477:CDS
ATTCGGGTTAATCAAAAGCG+GGG 0.673297 3.3:+72716474 None:intergenic
CATCAGCCCTGTTCAAACCA+GGG 0.679855 3.3:+72716200 None:intergenic
TTCCCCGGTTCGAACCAGAA+CGG 0.681205 3.3:+72716233 None:intergenic
AGATTCGGGTTAATCAAAAG+CGG 0.681647 3.3:+72716472 None:intergenic
CCGCAAAATGGGTATTACAG+GGG 0.729401 3.3:-72716319 MS.gene75477:CDS
GACGGAAAGAAGAAGAAACG+TGG 0.737712 3.3:-72716550 MS.gene75477:CDS
GGAAAGAAGAAGAAACGTGG+CGG 0.756525 3.3:-72716547 MS.gene75477:CDS
GTATTGTTGAGGAAGCGCCG+GGG 0.806884 3.3:-72716387 MS.gene75477:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAGAAGAGTTTGAATAAAC+AGG - chr3.3:72716458-72716477 MS.gene75477:CDS 25.0%
TGCAAAACCTGATTACAAAA+AGG - chr3.3:72716365-72716384 MS.gene75477:CDS 30.0%
! ACAAAAGCCTTTTTGTAATC+AGG + chr3.3:72716375-72716394 None:intergenic 30.0%
AAAGGTTCGAACATTGAACA+TGG - chr3.3:72716308-72716327 MS.gene75477:CDS 35.0%
AACACGCTTGATTTCAATCT+TGG + chr3.3:72716259-72716278 None:intergenic 35.0%
ATGAGAATAAGAACACTCGT+GGG - chr3.3:72716532-72716551 MS.gene75477:CDS 35.0%
ATTTCAATCTTGGAAGCTGA+TGG + chr3.3:72716249-72716268 None:intergenic 35.0%
GAGTTTGAATAAACAGGCTA+TGG - chr3.3:72716464-72716483 MS.gene75477:CDS 35.0%
TCTCGAATCGGAAAAAGATT+CGG + chr3.3:72716426-72716445 None:intergenic 35.0%
TTTGAATAAACAGGCTATGG+CGG - chr3.3:72716467-72716486 MS.gene75477:CDS 35.0%
! AGATTCGGGTTAATCAAAAG+CGG + chr3.3:72716411-72716430 None:intergenic 35.0%
AAAATGGGTATTACAGGGGA+GGG - chr3.3:72716565-72716584 MS.gene75477:CDS 40.0%
AATCTTGGAAGCTGATGGAA+TGG + chr3.3:72716244-72716263 None:intergenic 40.0%
CTCGAATCGGAAAAAGATTC+GGG + chr3.3:72716425-72716444 None:intergenic 40.0%
GAGAGCGATTTCATGTATGT+CGG + chr3.3:72716217-72716236 None:intergenic 40.0%
GATGAGAATAAGAACACTCG+TGG - chr3.3:72716531-72716550 MS.gene75477:CDS 40.0%
GATTGAAATCAAGCGTGTTC+TGG - chr3.3:72716260-72716279 MS.gene75477:CDS 40.0%
GATTTCATGTATGTCGGTGA+AGG + chr3.3:72716211-72716230 None:intergenic 40.0%
GTTCGAACATTGAACATGGA+CGG - chr3.3:72716312-72716331 MS.gene75477:CDS 40.0%
TCCTCTTCAATCTCTCGAAT+CGG + chr3.3:72716438-72716457 None:intergenic 40.0%
! ATTCGGGTTAATCAAAAGCG+GGG + chr3.3:72716409-72716428 None:intergenic 40.0%
! GATTCGGGTTAATCAAAAGC+GGG + chr3.3:72716410-72716429 None:intergenic 40.0%
AGCCGCAAAATGGGTATTAC+AGG - chr3.3:72716559-72716578 MS.gene75477:CDS 45.0%
AGTTTCCCTGGAGTAACATG+AGG - chr3.3:72716715-72716734 MS.gene75477:CDS 45.0%
CAAAATGGGTATTACAGGGG+AGG - chr3.3:72716564-72716583 MS.gene75477:CDS 45.0%
CCGCAAAATGGGTATTACAG+GGG - chr3.3:72716561-72716580 MS.gene75477:CDS 45.0%
CTACGGATTCGATGTCGAAA+AGG - chr3.3:72716290-72716309 MS.gene75477:CDS 45.0%
CTGGTAGGAGTAATGCTACT+AGG - chr3.3:72716739-72716758 MS.gene75477:CDS 45.0%
GACGGAAAGAAGAAGAAACG+TGG - chr3.3:72716330-72716349 MS.gene75477:CDS 45.0%
GCCGCAAAATGGGTATTACA+GGG - chr3.3:72716560-72716579 MS.gene75477:CDS 45.0%
GGAAAGAAGAAGAAACGTGG+CGG - chr3.3:72716333-72716352 MS.gene75477:CDS 45.0%
GTGGGTTTAAGCCGCAAAAT+GGG - chr3.3:72716550-72716569 MS.gene75477:CDS 45.0%
TATGGCGGCTAGTATTGTTG+AGG - chr3.3:72716482-72716501 MS.gene75477:CDS 45.0%
TCGAATCTGTTTGCGAGTTC+TGG - chr3.3:72716603-72716622 MS.gene75477:CDS 45.0%
TGTATGTCGGTGAAGGTTGT+AGG + chr3.3:72716204-72716223 None:intergenic 45.0%
TTCGACATCGAATCCGTAGA+GGG + chr3.3:72716289-72716308 None:intergenic 45.0%
TTTCGACATCGAATCCGTAG+AGG + chr3.3:72716290-72716309 None:intergenic 45.0%
! CCCCTGTAATACCCATTTTG+CGG + chr3.3:72716564-72716583 None:intergenic 45.0%
! TAGTATTGTTGAGGAAGCGC+CGG - chr3.3:72716491-72716510 MS.gene75477:CDS 45.0%
! TCCGATTCGAGAGATTGAAG+AGG - chr3.3:72716434-72716453 MS.gene75477:CDS 45.0%
ACCAGTCCTCATGTTACTCC+AGG + chr3.3:72716724-72716743 None:intergenic 50.0%
CATCAGCCCTGTTCAAACCA+GGG + chr3.3:72716683-72716702 None:intergenic 50.0%
CCAGTCCTCATGTTACTCCA+GGG + chr3.3:72716723-72716742 None:intergenic 50.0%
CGTGGGTTTAAGCCGCAAAA+TGG - chr3.3:72716549-72716568 MS.gene75477:CDS 50.0%
GCAGGAGAAGCTTGATTGGT+AGG + chr3.3:72716182-72716201 None:intergenic 50.0%
GTAGGCAGGAGAAGCTTGAT+TGG + chr3.3:72716186-72716205 None:intergenic 50.0%
GTTCTGCTGCAAAGTTTCCC+TGG - chr3.3:72716703-72716722 MS.gene75477:CDS 50.0%
TCAAGCCAATGACTCTTCCC+CGG + chr3.3:72716513-72716532 None:intergenic 50.0%
! GGAGTAACATGAGGACTGGT+AGG - chr3.3:72716724-72716743 MS.gene75477:CDS 50.0%
! TGAACGCCCTGGTTTGAACA+GGG - chr3.3:72716674-72716693 MS.gene75477:CDS 50.0%
! TTGAACGCCCTGGTTTGAAC+AGG - chr3.3:72716673-72716692 MS.gene75477:CDS 50.0%
!! AGTATTGTTGAGGAAGCGCC+GGG - chr3.3:72716492-72716511 MS.gene75477:CDS 50.0%
!! CGTGTTCTGGAATCCCTCTA+CGG - chr3.3:72716273-72716292 MS.gene75477:CDS 50.0%
!! GCGAGTTCTGGTTCGAATCA+GGG - chr3.3:72716615-72716634 MS.gene75477:CDS 50.0%
!! GTTCTGGTTCGAATCAGGGA+CGG - chr3.3:72716619-72716638 MS.gene75477:CDS 50.0%
!! TGCGAGTTCTGGTTCGAATC+AGG - chr3.3:72716614-72716633 MS.gene75477:CDS 50.0%
!! TTGACCGTTCTGGTTCGAAC+CGG - chr3.3:72716643-72716662 MS.gene75477:CDS 50.0%
AGGGGAGGGTTTGATCGTTC+TGG - chr3.3:72716579-72716598 MS.gene75477:CDS 55.0%
CCATCAGCCCTGTTCAAACC+AGG + chr3.3:72716684-72716703 None:intergenic 55.0%
CCCTGGAGTAACATGAGGAC+TGG - chr3.3:72716720-72716739 MS.gene75477:CDS 55.0%
TGTCGGTGAAGGTTGTAGGC+AGG + chr3.3:72716200-72716219 None:intergenic 55.0%
! CCTGGTTTGAACAGGGCTGA+TGG - chr3.3:72716681-72716700 MS.gene75477:CDS 55.0%
! TTCCCCGGTTCGAACCAGAA+CGG + chr3.3:72716650-72716669 None:intergenic 55.0%
!! GTATTGTTGAGGAAGCGCCG+GGG - chr3.3:72716493-72716512 MS.gene75477:CDS 55.0%
!! TGACCGTTCTGGTTCGAACC+GGG - chr3.3:72716644-72716663 MS.gene75477:CDS 55.0%
CAGGGACGGTTTGACCGTTC+TGG - chr3.3:72716633-72716652 MS.gene75477:CDS 60.0%
! CAGGGCGTTCAAACCTTCCC+CGG + chr3.3:72716665-72716684 None:intergenic 60.0%
!! AAGCGCCGGGGAAGAGTCAT+TGG - chr3.3:72716505-72716524 MS.gene75477:CDS 60.0%
!! GACCGTTCTGGTTCGAACCG+GGG - chr3.3:72716645-72716664 MS.gene75477:CDS 60.0%
!! GTTCTGGTTCGAACCGGGGA+AGG - chr3.3:72716649-72716668 MS.gene75477:CDS 60.0%
CGGGGAAGGTTTGAACGCCC+TGG - chr3.3:72716663-72716682 MS.gene75477:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 72716138 72716764 72716138 ID=MS.gene75477
chr3.3 mRNA 72716138 72716764 72716138 ID=MS.gene75477.t1;Parent=MS.gene75477
chr3.3 exon 72716138 72716764 72716138 ID=MS.gene75477.t1.exon1;Parent=MS.gene75477.t1
chr3.3 CDS 72716138 72716764 72716138 ID=cds.MS.gene75477.t1;Parent=MS.gene75477.t1
Gene Sequence

>MS.gene75477

ATGGGAAGCAGACTAGGAAGAAGAGTAGTGTACTTCGCAAACCTACCAATCAAGCTTCTCCTGCCTACAACCTTCACCGACATACATGAAATCGCTCTCAAAACCATTCCATCAGCTTCCAAGATTGAAATCAAGCGTGTTCTGGAATCCCTCTACGGATTCGATGTCGAAAAGGTTCGAACATTGAACATGGACGGAAAGAAGAAGAAACGTGGCGGAATCGTGATTGCAAAACCTGATTACAAAAAGGCTTTTGTAACGTTAAAAACCCCGCTTTTGATTAACCCGAATCTTTTTCCGATTCGAGAGATTGAAGAGGATAAGAAGAGTTTGAATAAACAGGCTATGGCGGCTAGTATTGTTGAGGAAGCGCCGGGGAAGAGTCATTGGCTTGATGAGAATAAGAACACTCGTGGGTTTAAGCCGCAAAATGGGTATTACAGGGGAGGGTTTGATCGTTCTGGTTCGAATCTGTTTGCGAGTTCTGGTTCGAATCAGGGACGGTTTGACCGTTCTGGTTCGAACCGGGGAAGGTTTGAACGCCCTGGTTTGAACAGGGCTGATGGTTCTGCTGCAAAGTTTCCCTGGAGTAACATGAGGACTGGTAGGAGTAATGCTACTAGGTAG

Protein sequence

>MS.gene75477.t1

MGSRLGRRVVYFANLPIKLLLPTTFTDIHEIALKTIPSASKIEIKRVLESLYGFDVEKVRTLNMDGKKKKRGGIVIAKPDYKKAFVTLKTPLLINPNLFPIREIEEDKKSLNKQAMAASIVEEAPGKSHWLDENKNTRGFKPQNGYYRGGFDRSGSNLFASSGSNQGRFDRSGSNRGRFERPGLNRADGSAAKFPWSNMRTGRSNATR